desiccation

Summary

Summary: Removal of moisture from a substance (chemical, food, tissue, etc.).

Top Publications

  1. ncbi Desiccation tolerance in human cells
    I Puhlev
    UCSD Cancer Center, La Jolla, California 92093-0912, USA
    Cryobiology 42:207-17. 2001
  2. ncbi The continuing conundrum of the LEA proteins
    Alan Tunnacliffe
    Institute of Biotechnology, University of Cambridge, Cambridge, UK
    Naturwissenschaften 94:791-812. 2007
  3. ncbi NHEJ protects mycobacteria in stationary phase against the harmful effects of desiccation
    Robert S Pitcher
    Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton BN1 9RQ, UK
    DNA Repair (Amst) 6:1271-6. 2007
  4. ncbi Effects of starvation and desiccation on energy metabolism in desert and mesic Drosophila
    M T Marron
    Department of Ecology and Evolutionary Biology, University of Arizona, 1041 E Lowell St, Tucson, AZ 85721, USA
    J Insect Physiol 49:261-70. 2003
  5. pmc A sister group contrast using untargeted global metabolomic analysis delineates the biochemical regulation underlying desiccation tolerance in Sporobolus stapfianus
    Melvin J Oliver
    U S Department of Agriculture Agricultural Research Service, Plant Genetic Research Unit, University of Missouri, Columbia, Missouri 65211, USA
    Plant Cell 23:1231-48. 2011
  6. pmc Adaptation to aridity in the malaria mosquito Anopheles gambiae: chromosomal inversion polymorphism and body size influence resistance to desiccation
    Caroline Fouet
    Institut de Recherche pour le Developpement, UMR MIVEGEC, UM1, UM2, CNRS 5290, IRD 224, Montpellier, France
    PLoS ONE 7:e34841. 2012
  7. ncbi Trehalose renders the dauer larva of Caenorhabditis elegans resistant to extreme desiccation
    Cihan Erkut
    Max Planck Institute of Molecular Cell Biology and Genetics, Dresden 01307, Germany
    Curr Biol 21:1331-6. 2011
  8. ncbi Molecular mechanisms of desiccation tolerance in resurrection plants
    Tsanko S Gechev
    Department of Plant Physiology and Plant Molecular Biology, University of Plovdiv, Bulgaria
    Cell Mol Life Sci 69:3175-86. 2012
  9. ncbi C4 grasses prosper as carbon dioxide eliminates desiccation in warmed semi-arid grassland
    Jack A Morgan
    USDA ARS, Rangeland Resources Research Unit and Northern Plains Area, Fort Collins, Colorado 80526, USA
    Nature 476:202-5. 2011
  10. pmc Structure and function of a mitochondrial late embryogenesis abundant protein are revealed by desiccation
    Dimitri Tolleter
    Unité Mixte de Recherche 1191, Physiologie Moléculaire des Semences, Université d Angers Institut National d Horticulture Institut National de la Recherche Agronomique, Angers F 49045, France
    Plant Cell 19:1580-9. 2007

Detail Information

Publications352 found, 100 shown here

  1. ncbi Desiccation tolerance in human cells
    I Puhlev
    UCSD Cancer Center, La Jolla, California 92093-0912, USA
    Cryobiology 42:207-17. 2001
    ..The most important finding is that vacuum greatly enhances the ability of human cells in culture to withstand desiccation. In fact, cells dried slowly and stored under vacuum are able to withstand desiccation even in the absence of ..
  2. ncbi The continuing conundrum of the LEA proteins
    Alan Tunnacliffe
    Institute of Biotechnology, University of Cambridge, Cambridge, UK
    Naturwissenschaften 94:791-812. 2007
    ..The challenge now facing researchers investigating these enigmatic proteins is to make sense of the various in vitro defined functions in the living cell: Are the LEA proteins truly multi-talented, or are they still just misunderstood?..
  3. ncbi NHEJ protects mycobacteria in stationary phase against the harmful effects of desiccation
    Robert S Pitcher
    Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton BN1 9RQ, UK
    DNA Repair (Amst) 6:1271-6. 2007
    ..Similarly, NHEJ-mutant strains were more sensitive to prolonged desiccation than wild-type M. smegmatis...
  4. ncbi Effects of starvation and desiccation on energy metabolism in desert and mesic Drosophila
    M T Marron
    Department of Ecology and Evolutionary Biology, University of Arizona, 1041 E Lowell St, Tucson, AZ 85721, USA
    J Insect Physiol 49:261-70. 2003
    ..laboratory experiments have revealed that energy storage patterns differ between populations selected for desiccation and starvation...
  5. pmc A sister group contrast using untargeted global metabolomic analysis delineates the biochemical regulation underlying desiccation tolerance in Sporobolus stapfianus
    Melvin J Oliver
    U S Department of Agriculture Agricultural Research Service, Plant Genetic Research Unit, University of Missouri, Columbia, Missouri 65211, USA
    Plant Cell 23:1231-48. 2011
    ..The desiccation-tolerant (DT) Sporobolus stapfianus and the desiccation-sensitive (DS) Sporobolus pyramidalis formed a sister ..
  6. pmc Adaptation to aridity in the malaria mosquito Anopheles gambiae: chromosomal inversion polymorphism and body size influence resistance to desiccation
    Caroline Fouet
    Institut de Recherche pour le Developpement, UMR MIVEGEC, UM1, UM2, CNRS 5290, IRD 224, Montpellier, France
    PLoS ONE 7:e34841. 2012
    ..To test this hypothesis, we compared the desiccation resistance of alternative karyotypes from a colonised 2Rb/2La polymorphic population of A...
  7. ncbi Trehalose renders the dauer larva of Caenorhabditis elegans resistant to extreme desiccation
    Cihan Erkut
    Max Planck Institute of Molecular Cell Biology and Genetics, Dresden 01307, Germany
    Curr Biol 21:1331-6. 2011
    ..By means of infrared spectroscopy, we can now distinguish a "dry, yet alive" larva from a "dry and dead" one...
  8. ncbi Molecular mechanisms of desiccation tolerance in resurrection plants
    Tsanko S Gechev
    Department of Plant Physiology and Plant Molecular Biology, University of Plovdiv, Bulgaria
    Cell Mol Life Sci 69:3175-86. 2012
    ..plants are a small but diverse group of land plants characterized by their tolerance to extreme drought or desiccation. They have the unique ability to survive months to years without water, lose most of the free water in their ..
  9. ncbi C4 grasses prosper as carbon dioxide eliminates desiccation in warmed semi-arid grassland
    Jack A Morgan
    USDA ARS, Rangeland Resources Research Unit and Northern Plains Area, Fort Collins, Colorado 80526, USA
    Nature 476:202-5. 2011
    Global warming is predicted to induce desiccation in many world regions through increases in evaporative demand. Rising CO(2) may counter that trend by improving plant water-use efficiency...
  10. pmc Structure and function of a mitochondrial late embryogenesis abundant protein are revealed by desiccation
    Dimitri Tolleter
    Unité Mixte de Recherche 1191, Physiologie Moléculaire des Semences, Université d Angers Institut National d Horticulture Institut National de la Recherche Agronomique, Angers F 49045, France
    Plant Cell 19:1580-9. 2007
    Few organisms are able to withstand desiccation stress; however, desiccation tolerance is widespread among plant seeds...
  11. ncbi The effects of acclimation on thermal tolerance, desiccation resistance and metabolic rate in Chirodica chalcoptera (Coleoptera: Chrysomelidae)
    John S Terblanche
    Spatial, Physiological, and Conservation Ecology Group, Department of Botany and Zoology, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa
    J Insect Physiol 51:1013-23. 2005
    ..We report the effects of temperature acclimation on thermal tolerance, desiccation rate and metabolic rate for adult Chirodica chalcoptera (Coleoptera: Chrysomelidae) collected from Protea ..
  12. ncbi Molecular mechanisms of desiccation tolerance in the resurrection glacial relic Haberlea rhodopensis
    Tsanko S Gechev
    Department of Plant Physiology and Plant Molecular Biology, University of Plovdiv, 24 Tsar Assen Str, Plovdiv, 4000, Bulgaria
    Cell Mol Life Sci 70:689-709. 2013
    Haberlea rhodopensis is a resurrection plant with remarkable tolerance to desiccation. Haberlea exposed to drought stress, desiccation, and subsequent rehydration showed no signs of damage or severe oxidative stress compared to untreated ..
  13. ncbi Dehydration-regulated processing of late embryogenesis abundant protein in a desiccation-tolerant nematode
    Kshamata Goyal
    Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
    FEBS Lett 579:4093-8. 2005
    Late embryogenesis abundant (LEA) proteins occur in desiccation-tolerant organisms, including the nematode Aphelenchus avenae, and are thought to protect other proteins from aggregation...
  14. pmc Hydrophilic protein associated with desiccation tolerance exhibits broad protein stabilization function
    Sohini Chakrabortee
    Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, United Kingdom
    Proc Natl Acad Sci U S A 104:18073-8. 2007
    ..One phylogenetically widespread adaptation is the presence of abundant, highly hydrophilic proteins in desiccation-tolerant organisms...
  15. pmc Comparative analysis of the heat stable proteome of radicles of Medicago truncatula seeds during germination identifies late embryogenesis abundant proteins associated with desiccation tolerance
    Julie Boudet
    Unité Mixte de Recherche 1191, Physiologie Moléculaire des Semences, Anjou Recherche Semences, 49045 Angers, France
    Plant Physiol 140:1418-36. 2006
    ..of Medicago truncatula seeds to investigate whether proteins can be identified that are specifically linked to desiccation tolerance (DT). Radicles were compared before and after emergence (2...
  16. ncbi Desiccation of the resurrection plant Haberlea rhodopensis at high temperature
    Gergana Mihailova
    Acad M Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Acad G Bonchev Str, Bl 21, 1113, Sofia, Bulgaria
    Photosynth Res 108:5-13. 2011
    ..activity of PSII and PSI and the rate of photosynthetic oxygen evolution, and these effects were stronger when desiccation was carried out at 38°C...
  17. ncbi Proteomics of desiccation tolerance during development and germination of maize embryos
    Hui Huang
    Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
    J Proteomics 75:1247-62. 2012
    Maize seeds were used to identify the key embryo proteins involved in desiccation tolerance during development and germination...
  18. ncbi Programming desiccation-tolerance: from plants to seeds to resurrection plants
    Jill M Farrant
    Department of Molecular and Cell Biology, University of Cape Town, Rondebosch 7701, South Africa
    Curr Opin Plant Biol 14:340-5. 2011
    b>Desiccation-tolerance (DT) evolved as the key solution to survival on land by the early algal ancestors of terrestrial plants...
  19. ncbi PSI-driven cyclic electron flow allows intertidal macro-algae Ulva sp. (Chlorophyta) to survive in desiccated conditions
    Shan Gao
    Tianjin Key Laboratory of Marine Resources and Chemistry, College of Marine Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, PR China
    Plant Cell Physiol 52:885-93. 2011
    ..performance of the photosystems and the changes in photosynthetic electron transport that occur during desiccation and rehydration of Ulva sp. The PSII activity in Ulva sp...
  20. ncbi Sip18 hydrophilin prevents yeast cell death during desiccation stress
    B Rodríguez-Porrata
    Department of Biochemistry and Biotechnology, University Rovira i Virgili, Tarragona, Spain
    J Appl Microbiol 112:512-25. 2012
    ..For this study, we performed a genetic screen of S. cerevisiae's deletion library for mutants sensitive to dehydration stress, with which we aimed to discover cell dehydration-tolerant genes...
  21. pmc Embryonic desiccation resistance in Aedes aegypti: presumptive role of the chitinized serosal cuticle
    Gustavo Lazzaro Rezende
    Laboratório de Fisiologia e Controle de Artrópodes Vetores, Instituto Oswaldo Cruz, FIOCRUZ and Laboratório de Entomologia, Instituto de Biologia do Exército, Rio de Janeiro, Brazil
    BMC Dev Biol 8:82. 2008
    ..problems concerning dengue transmission is that embryos of its main vector, the mosquito Aedes aegypti, resist desiccation, surviving several months under dry conditions...
  22. ncbi Cytoarchitecture of the desiccation-tolerant green alga Zygogonium ericetorum
    A Holzinger
    Department of Physiology and Cell Physiology of Alpine Plants, Institute of Botany, Sternwartestr 15, 6020 Innsbruck, Austria
    Protoplasma 243:15-24. 2010
    ..Water-soluble extracts were separated by high-pressure liquid chromatography that revealed two major compounds with UV-absorbing capacities...
  23. ncbi The role of lipid metabolism in the acquisition of desiccation tolerance in Craterostigma plantagineum: a comparative approach
    Francisco Gasulla
    Botánica and ICBIBE, Fac C Biológicas, Universitat de Valencia, C Dr Moliner 50, Burjassot, Valencia 46100, Spain Institute of Molecular Physiology and Biotechnology of Plants IMBIO, University of Bonn, Kirschallee 1, Bonn D 53115, Germany
    Plant J 75:726-41. 2013
    ..We analysed the lipid composition and the expression of genes involved in lipid biosynthesis in the desiccation-tolerant plant Craterostigma plantagineum...
  24. ncbi Anhydrobiosis in bacteria: from physiology to applications
    Armando Hernandez Garcia
    Department of Biochemistry, Research Group on Infection Diseases, Centro Nacional de Investigaciones Cientificas, Ave 25 y 158, Cubanacan, Playa, La Habana, Cuba
    J Biosci 36:939-50. 2011
    Anhydrobiosis is a phenomenon related to the partial or total desiccation of living organisms, keeping their vital functions after rehydration...
  25. pmc Quantitative genetic analysis suggests causal association between cuticular hydrocarbon composition and desiccation survival in Drosophila melanogaster
    B R Foley
    Department of Biological Sciences, University of Southern California, Los Angeles, CA 90007, USA
    Heredity (Edinb) 106:68-77. 2011
    Survival to low relative humidity is a complex adaptation, and many repeated instances of evolution to desiccation have been observed among Drosophila populations and species...
  26. ncbi Osmotically induced intracellular trehalose, but not glycine betaine accumulation promotes desiccation tolerance in Escherichia coli
    D T Welsh
    Dipartimento di Scienze Ambientali, Universita degli Studi di Parma, Italy
    FEMS Microbiol Lett 174:57-63. 1999
    ..However, trehalose alone cannot explain the intrinsically greater desiccation tolerance of stationary compared to exponential phase E...
  27. ncbi Tardigrade Resistance to Space Effects: first results of experiments on the LIFE-TARSE mission on FOTON-M3 (September 2007)
    Lorena Rebecchi
    Department of Animal Biology, University of Modena and Reggio Emilia, 41100, Modena, Italy
    Astrobiology 9:581-91. 2009
    ..During the flight mission, tardigrades molted, and females laid eggs. Several eggs hatched, and the newborns exhibited normal morphology and behavior...
  28. pmc Expression profiling and cross-species RNA interference (RNAi) of desiccation-induced transcripts in the anhydrobiotic nematode Aphelenchus avenae
    Wesley Reardon
    Department of Biology, National University of Ireland, Maynooth, CO, Kildare, Ireland
    BMC Mol Biol 11:6. 2010
    Some organisms can survive extreme desiccation by entering a state of suspended animation known as anhydrobiosis...
  29. ncbi Preparation and characterization of nanocrystals for solubility and dissolution rate enhancement of nifedipine
    J Hecq
    Laboratory of Pharmaceutics and Biopharmaceutics, Universite Libre de Bruxelles, Campus Plaine, CP 207, Boulevard du Triomphe, Brussels 1050, Belgium
    Int J Pharm 299:167-77. 2005
    ..The method being simple and easily scaled up, this approach should have a general applicability to many poorly water-soluble drug entities...
  30. pmc Mechanism and function of Drosophila capa GPCR: a desiccation stress-responsive receptor with functional homology to human neuromedinU receptor
    Selim Terhzaz
    Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom
    PLoS ONE 7:e29897. 2012
    ..Adult capaR RNAi transgenic flies also display resistance to desiccation. Thus, capaR acts in the key fluid-transporting tissue to regulate responses to desiccation stress in the fly.
  31. pmc Crucial role of extracellular polysaccharides in desiccation and freezing tolerance in the terrestrial cyanobacterium Nostoc commune
    Yoshiyuki Tamaru
    Division of Life Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, Kanazawa 920 1192, Japan
    Appl Environ Microbiol 71:7327-33. 2005
    ..In this study, the role of extracellular polysaccharides (EPS) in the desiccation tolerance of photosynthesis in N. commune was examined...
  32. ncbi Fungicidal effects of chemical disinfectants, UV light, desiccation and heat on the amphibian chytrid Batrachochytrium dendrobatidis
    Megan L Johnson
    School of Public Health and Tropical Medicine, James Cook University, Townsville, Queensland 4810, Australia
    Dis Aquat Organ 57:255-60. 2003
    ..was to evaluate the fungicidal effects of chemical disinfectants, sterilising ultraviolet (UV) light, heat and desiccation, using methods that were feasible for either disinfection in the field, in amphibian husbandry or in the ..
  33. pmc Effects of larval growth condition and water availability on desiccation resistance and its physiological basis in adult Anopheles gambiae sensu stricto
    Fred Aboagye-Antwi
    Centre for Applied Entomology and Parasitology, School of Life Sciences, Keele University, Keele, Staffordshire, UK
    Malar J 9:225. 2010
    ..Natural populations of the malaria mosquito Anopheles gambiae s.s. are exposed to large seasonal and daily fluctuations in relative humidity and temperature, which makes coping with drought a crucial aspect of their ecology...
  34. ncbi Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray
    Motoaki Seki
    Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3 1 1 Koyadai, Tsukuba 305 0074, Japan
    Plant J 31:279-92. 2002
    ....
  35. pmc OSM1/SYP61: a syntaxin protein in Arabidopsis controls abscisic acid-mediated and non-abscisic acid-mediated responses to abiotic stress
    Jianhua Zhu
    Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907 1165, USA
    Plant Cell 14:3009-28. 2002
    ..Together, our results demonstrate important roles of OSM1/SYP61 in osmotic stress tolerance and in the ABA regulation of stomatal responses...
  36. ncbi Transcriptome profiling uncovers metabolic and regulatory processes occurring during the transition from desiccation-sensitive to desiccation-tolerant stages in Medicago truncatula seeds
    Julia Buitink
    Unité Mixte de Recherche 1191 Physiologie Moléculaire des Semences, Université d Angers INH INRA, 16 Bd Lavoisier, 49045 Angers, France
    Plant J 47:735-50. 2006
    To investigate regulatory processes and protective mechanisms leading to desiccation tolerance (DT) in seeds, 16086-element microarrays were used to monitor changes in the transcriptome of desiccation-sensitive 3-mm-long radicles of ..
  37. ncbi Adaptations of higher plant cell walls to water loss: drought vs desiccation
    John P Moore
    Institute for Wine Biotechnology, Faculty of AgriSciences, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa
    Physiol Plant 134:237-45. 2008
    ..b>Desiccation tolerance, by contrast, is the evolution of cell walls that are capable of losing the majority of cellular ..
  38. pmc Development and application of a broadly sensitive dried-blood-spot-based genotyping assay for global surveillance of HIV-1 drug resistance
    Chunfu Yang
    Division of Global AIDS, International Laboratory Branch, DGHA, NCHHSTP, CDC, Mail Stop A 11, 1600 Clifton Road, Atlanta, GA 30333, USA
    J Clin Microbiol 48:3158-64. 2010
    ..Thus, the assay is likely to become a useful screening tool in the global resistance surveillance and monitoring of HIV-1 where multiple subtypes and CRFs are found...
  39. ncbi Protein oxidation: key to bacterial desiccation resistance?
    James K Fredrickson
    Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA
    ISME J 2:393-403. 2008
    ..between survival of dry-climate soil bacteria and the level of cellular protein oxidation induced by desiccation. Bacteria were isolated from surface soils of the shrub-steppe of the US Department of Energy's Hanford Site in ..
  40. pmc Transcriptional response of Saccharomyces cerevisiae to desiccation and rehydration
    Jatinder Singh
    Virginia Tech Center for Genomics, Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061, USA
    Appl Environ Microbiol 71:8752-63. 2005
    A transcriptional analysis of the response of Saccharomyces cerevisiae strain BY4743 to controlled air-drying (desiccation) and subsequent rehydration under minimal glucose conditions was performed...
  41. ncbi The survival of Listeria monocytogenes during long term desiccation is facilitated by sodium chloride and organic material
    Birte Fonnesbech Vogel
    Technical University of Denmark, National Food Institute, Søltofts Plads, Building 221, DK 2800 Kgs Lyngby, Denmark
    Int J Food Microbiol 140:192-200. 2010
    ..We hypothesised that tolerance to desiccation could be a factor in explaining the persistence of L...
  42. pmc Heat shock proteins and resistance to desiccation in congeneric land snails
    Tal Mizrahi
    Department of Biology, Technion, Haifa, 32000, Israel
    Cell Stress Chaperones 15:351-63. 2010
    ..We used experimental desiccation to test whether adaptation to different habitats affects HSP expression in two closely related Sphincterochila ..
  43. pmc Transcriptome survey of the anhydrobiotic tardigrade Milnesium tardigradum in comparison with Hypsibius dujardini and Richtersius coronifer
    Brahim Mali
    Molecular Biology and Functional Genomics, University of Applied Sciences Wildau, Bahnhofstrasse 1, 15745 Wildau, Germany
    BMC Genomics 11:168. 2010
    The phenomenon of desiccation tolerance, also called anhydrobiosis, involves the ability of an organism to survive the loss of almost all cellular water without sustaining irreversible damage...
  44. ncbi Protective mechanism of desiccation tolerance in Reaumuria soongorica: leaf abscission and sucrose accumulation in the stem
    Yubing Liu
    School of Life Sciences, Lanzhou University, Lanzhou, 730000, China
    Sci China C Life Sci 50:15-21. 2007
    ..a perennial semi-shrub, is widely found in semi-arid areas in northwestern China and can survive severe desiccation of its vegetative organs. In order to study the protective mechanism of desiccation tolerance in R...
  45. ncbi Desiccation tolerance of spermatozoa dried at ambient temperature: production of fetal mice
    Sankha Bhowmick
    Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, Boston 02115, USA
    Biol Reprod 68:1779-86. 2003
    Long-term preservation of mouse sperm by desiccation is economically and logistically attractive. The current investigation is a feasibility study of the preservation of mouse sperm by convective drying in an inert gas (nitrogen)...
  46. ncbi Preservation of micro-organisms by drying; a review
    C A Morgan
    BTF Pty Ltd, PO Box 599, North Ryde BC, NSW 1670, Australia
    J Microbiol Methods 66:183-93. 2006
    ..Also, particular attention has been paid to the use of freeze drying and the protective matrices used to improve microbial cell viability after drying...
  47. ncbi Mouse sperm desiccated and stored in trehalose medium without freezing
    Lynda K McGinnis
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Biol Reprod 73:627-33. 2005
    ..b>Desiccation and storage in medium with trehalose significantly increased sperm developmental potential compared to medium ..
  48. ncbi Impact of environmental stress desiccation, acidity, alkalinity, heat or cold on antibiotic susceptibility of Cronobacter sakazakii
    Anas A Al-Nabulsi
    Department of Nutrition and Food Technology, Jordan University of Science and Technology, Irbid, Jordan
    Int J Food Microbiol 146:137-43. 2011
    ..5 for 30 min or 11.25 for 5 min), cold (4°C for 24h), heat (55°C for 5 min), and desiccation (cells were dried at 40°C for 2h and held at 21°C for 4 d) stresses on susceptibility of five isolated ..
  49. ncbi Quality by design approach in the optimization of the spray-drying process
    Arnaud Baldinger
    Faculty of Pharmaceutical Sciences, Department of Pharmaceutics and Analytical Chemistry, University of Copenhagen, Copenhagen, Denmark
    Pharm Dev Technol 17:389-97. 2012
    ..The aim of this study was to illustrate the influence of the processing parameters, inlet temperature, atomization air flow rate and feed flow rate, on critical quality attributes of spray-dried powders using design of experiments (DoE)...
  50. pmc Characterization of enzymatic antioxidants in the lichen Ramalina lacera and their response to rehydration
    Lior Weissman
    Department of Plant Sciences, George S Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel
    Appl Environ Microbiol 71:6508-14. 2005
    ..The lifestyle of most lichens is composed of alternating periods of desiccation with low metabolic activity and hydration that induces increase in their metabolism...
  51. ncbi Mechanisms to avoid photoinhibition in a desiccation-tolerant cyanobacterium, Nostoc commune
    Shin ya Fukuda
    Department of Life Science, Graduate School of Life Science, University of Hyogo, 3 2 1 Kouto, Kamigori cho, Ako gun, Hyogo, 678 1297 Japan
    Plant Cell Physiol 49:488-92. 2008
    A desiccation-tolerant cyanobacterium, Nostoc commune, shows unique responses to dehydration...
  52. ncbi Membrane phase behavior of Escherichia coli during desiccation, rehydration, and growth recovery
    Cally M Scherber
    Biology Program, University of Minnesota, Minneapolis, MN 55445, USA
    Biochim Biophys Acta 1788:2427-35. 2009
    ..phase behavior, as identified by membrane phase transition temperature (T(m)), of Escherichia coli during desiccation and rehydration. Extensive cell desiccation (1 week at 20%-40% RH) resulted in an increase in T(m) from 8.4+/-1...
  53. ncbi Impact of body melanisation on desiccation resistance in montane populations of D. melanogaster: Analysis of seasonal variation
    Ravi Parkash
    Department of Genetics, Maharshi Dayanand University, Rohtak, India
    J Insect Physiol 55:898-908. 2009
    ..localities of subtropical regions, winter is the dry season and ectothermic drosophilids are expected to evolve desiccation resistance to cope with drier climatic conditions. An analysis of six montane populations (600-2226m) of D...
  54. ncbi Photosynthetic recovery following desiccation of desert green algae (Chlorophyta) and their aquatic relatives
    Dennis W Gray
    Department of Ecology and Evolutionary Biology, University of Connecticut, Storrs, CT 06269 3043, USA
    Plant Cell Environ 30:1240-55. 2007
    ..We examined the photorecovery of phylogenetically matched desert and aquatic algae after desiccation in darkness and under illumination...
  55. pmc Exploring the mechanism of Physcomitrella patens desiccation tolerance through a proteomic strategy
    Xiao Qin Wang
    College of Life Sciences, Capital Normal University, Beijing 100048, China
    Plant Physiol 149:1739-50. 2009
    The moss Physcomitrella patens has been shown to tolerate abiotic stresses, including salinity, cold, and desiccation. To better understand this plant's mechanism of desiccation tolerance, we have applied cellular and proteomic analyses...
  56. pmc Phylogenetic analyses in cornus substantiate ancestry of xylem supercooling freezing behavior and reveal lineage of desiccation related proteins
    Dale T Karlson
    Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907 1165, USA
    Plant Physiol 135:1654-65. 2004
    ..Dehydrin-like proteins were limited to neither nonsupercooling species nor to those that possess extreme freeze tolerance...
  57. ncbi Fertilization and development in vitro of bovine oocytes following intracytoplasmic injection of heat-dried sperm heads
    Kyung Bon Lee
    Department of Animal Science, The Graduate School of Natural Science and Technology, Okayama University, Okayama 700 8530, Japan
    Biol Reprod 74:146-52. 2006
    ..These results demonstrate that bovine oocytes can be fertilized with heat-dried spermatozoa and that the fertilized oocytes can develop at least to the blastocyst stage...
  58. ncbi Physiological responses of the halophilic archaeon Halobacterium sp. strain NRC1 to desiccation and gamma irradiation
    Molly Kottemann
    Department of Cell Biology and Molecular Genetics, University of Maryland, 3221 H J Patterson Hall, College Park, MD 20742, USA
    Extremophiles 9:219-27. 2005
    We report that the halophilic archaeon Halobacterium sp. strain NRC-1 is highly resistant to desiccation, high vacuum and 60Co gamma irradiation. Halobacterium sp...
  59. ncbi Microbial communities in streambed sediments recovering from desiccation
    Jürgen Marxsen
    Limnologische Fluss Station des Max Planck Instituts für Limnologie, Schlitz, Germany
    FEMS Microbiol Ecol 71:374-86. 2010
    ..Here, much more intense drying resulted in a more distinct change and reduction of the microbial community, responsible for slower recovery of structure and functions...
  60. pmc Catalytic and chaperone-like functions in an intrinsically disordered protein associated with desiccation tolerance
    Sohini Chakrabortee
    Department of Chemical Engineering and Biotechnology, Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, United Kingdom
    Proc Natl Acad Sci U S A 107:16084-9. 2010
    ..We show here that anhydrin is a moonlighting protein with two novel, independent functions relating to desiccation tolerance...
  61. pmc Late embryogenesis abundant proteins protect human hepatoma cells during acute desiccation
    Shumin Li
    Division of Cellular, Developmental, and Integrative Biology, Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
    Proc Natl Acad Sci U S A 109:20859-64. 2012
    Expression of late embryogenesis abundant (LEA) proteins is highly correlated with desiccation tolerance in anhydrobiotic animals, selected land plants, and bacteria...
  62. ncbi Phenomic and transcriptomic analyses reveal that autophagy plays a major role in desiccation tolerance in Saccharomyces cerevisiae
    Sooraj Ratnakumar
    Department of Chemical Engineering and Biotechnology, Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
    Mol Biosyst 7:139-49. 2011
    Saccharomyces cerevisiae can survive extreme desiccation, but the molecular mechanisms are poorly understood...
  63. ncbi Desiccation of adhering and biofilm Listeria monocytogenes on stainless steel: Survival and transfer to salmon products
    Lisbeth Truelstrup Hansen
    Food Science Program, Department of Process Engineering and Applied Science, Dalhousie University, 1360 Barrington Street, Halifax, NS B3J2X4, Canada
    Int J Food Microbiol 146:88-93. 2011
    ..The objective of this study was to examine survival during desiccation (43% RH and 15 °C) of biofilm L...
  64. ncbi A cDNA-based comparison of dehydration-induced proteins (dehydrins) in barley and corn
    T J Close
    CSIRO Division of Plant Industry, Canberra, ACT, Australia
    Plant Mol Biol 13:95-108. 1989
    ..The amino acid sequence of tryptic peptides from purified dehydration-induced proteins of corn established that the corn cDNAs correspond to a protein that is produced in abundance during the response of corn seedlings to dehydration...
  65. ncbi Detection of Puumala and Rift Valley Fever virus by quantitative RT-PCR and virus viability tests in samples of blood dried and stored on filter paper
    Jonas Näslund
    Department of Clinical Microbiology, Division of Infectious Diseases, Umea University, SE 901 85 Umea, Sweden
    J Virol Methods 178:186-90. 2011
    ..This approach might facilitate sampling and analysis of other RNA viruses from human or animal sources and could be used for field studies in remote areas or in developing countries...
  66. pmc Resistance and resilience of benthic biofilm communities from a temperate saltmarsh to desiccation and rewetting
    Boyd A McKew
    Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, UK
    ISME J 5:30-41. 2011
    Periods of desiccation and rewetting are regular, yet stressful events encountered by saltmarsh microbial communities...
  67. pmc β-Aminobutyric acid increases abscisic acid accumulation and desiccation tolerance and decreases water use but fails to improve grain yield in two spring wheat cultivars under soil drying
    Yan lei DU
    State Key Laboratory of Grassland Agroecosystem, Institute of Arid Agroecology, School of Life Sciences, Lanzhou University Lanzhou 730000, Gansu Province China
    J Exp Bot 63:4849-60. 2012
    ..soil drying, and its relationship with the accumulation of abscisic acid (ABA), water use, grain yield, and desiccation tolerance in two spring wheat (Triticum aestivum L...
  68. ncbi Dehydrin metabolism is altered during seed osmopriming and subsequent germination under chilling and desiccation in Spinacia oleracea L. cv. Bloomsdale: possible role in stress tolerance
    Keting Chen
    Department of Horticulture, Iowa State University, Ames, IA 50011 1100, USA
    Plant Sci 183:27-36. 2012
    ..e. chilling and desiccation) in spinach (Spinacia oleracea L. cv. Bloomsdale) seeds...
  69. ncbi Multiple dissipation components of excess light energy in dry lichen revealed by ultrafast fluorescence study at 5 K
    Hirohisa Miyake
    Division of Material Science Physics, Graduate School of Science, Nagoya University, Nagoya 464 8602, Japan
    Photosynth Res 110:39-48. 2011
    ..to the emergence of a rapid quenching state in the peripheral-antenna system of photosystem II (PS II) on desiccation. This is an extremely effective protection mechanism of PS II under desiccation, which lichens have developed ..
  70. pmc Trehalose protects corneal epithelial cells from death by drying
    T Matsuo
    Department of Ophthalmology, Okayama University Medical School, Okayama City 700 8558, Japan
    Br J Ophthalmol 85:610-2. 2001
    This study was designed to examine whether trehalose could protect corneal epithelial cells in culture from death by desiccation in order to test trehalose as a potential new eye drop for dry eye syndrome.
  71. ncbi Photoprotection of green plants: a mechanism of ultra-fast thermal energy dissipation in desiccated lichens
    Ulrich Heber
    Julius von Sachs Institut fur Biowissenschaften, Universitat Wurzburg, Julius von Sachs Platz 2, 97082 Wurzburg, Germany
    Planta 228:641-50. 2008
    In order to survive sunlight in the absence of water, desiccation-tolerant green plants need to be protected against photooxidation...
  72. ncbi Changes in body melanisation and desiccation resistance in highland vs. lowland populations of D. melanogaster
    Ravi Parkash
    Department of Biochemistry and Genetics, Maharshi Dayanand University, Rohtak 124001, India
    J Insect Physiol 54:1050-6. 2008
    ..samples of Drosophila melanogaster from five highland localities showed parallel changes in melanisation and desiccation resistance in darker versus lighter phenotypes, i.e...
  73. pmc Nitric oxide enhances desiccation tolerance of recalcitrant Antiaris toxicaria seeds via protein S-nitrosylation and carbonylation
    Xuegui Bai
    Key Laboratory of Biodiversity and Biogeography, Institute of Tibet Plateau Research at Kunming, Chinese Academy of Science, Kunming, China
    PLoS ONE 6:e20714. 2011
    ..ascorbate-glutathione cycle can be used for the detoxification of ROS, but their roles in the seed response to desiccation remain poorly understood...
  74. ncbi Lubricating agents differ in their protection of cultured human epithelial cells against desiccation
    Katharina Paulsen
    Institute of Anatomy and Cell Biology, Ernst Moritz Arndt University, Ferdinand Sauerbruch Strasse, Greifswald, Germany
    Med Sci Monit 14:PI12-16. 2008
    ..In dry eye, as a disease of the ocular surface, the instillation of artificial tears should compensate for the deficit in wetting and protect the mucosa against drying...
  75. ncbi Bead layering as a process to stabilize nanosuspensions: influence of drug hydrophobicity on nanocrystal reagglomeration following in-vitro release from sugar beads
    Pieterjan Kayaert
    Department of Pharmaceutical Sciences, Laboratory for Pharmacotechnology and Biopharmacy, Katholieke Universiteit Leuven, Leuven, Belgium
    J Pharm Pharmacol 63:1446-53. 2011
    ..The second aim was to see how those physicochemical properties affect the coating process and the subsequent in-vitro dissolution process...
  76. pmc Patterns in fish radiation are compatible with Pleistocene desiccation of Lake Victoria and 14,600 year history for its cichlid species flock
    Ole Seehausen
    Department of Biological Sciences, Molecular and Evolutionary Ecology Group, University of Hull, Hull HU6 7RX, UK
    Proc Biol Sci 269:491-7. 2002
    Geophysical data are currently being interpreted as evidence for a late Pleistocene desiccation of Lake Victoria and its refilling 14,600 years ago...
  77. pmc Entrapment of conjunctival goblet cells by desiccation-induced cornification
    Rosa Maria Corrales
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas 77030, USA
    Invest Ophthalmol Vis Sci 52:3492-9. 2011
    ..To evaluate the effects of desiccating stress on conjunctival goblet cell density and morphology and the expression of cornified envelope precursors by the ocular surface epithelia...
  78. pmc From Avicennia to Zizania: seed recalcitrance in perspective
    Patricia Berjak
    School of Biological and Conservation Sciences, University of KwaZulu Natal, Durban, South Africa
    Ann Bot 101:213-28. 2008
    Considered only in terms of tolerance of, or sensitivity to, desiccation (which is an oversimplification), orthodox seeds are those which tolerate dehydration and are storable in this condition, while highly recalcitrant seeds are ..
  79. ncbi Spectacular improvement of X-ray diffraction through fast desiccation of protein crystals
    Chantal Abergel
    Information Genomique et Structurale, UPR2589 CNRS, IBSM, 31 Chemin Joseph Aiguier, 13402 Marseille, Cedex 20, France
    Acta Crystallogr D Biol Crystallogr 60:1413-6. 2004
    ..In addition to being a 'last resort' procedure for experimentalists struggling with non-diffracting crystals, this puzzling effect constitutes one more challenging problem for theoretical protein crystallographers...
  80. ncbi Curling during desiccation protects the foliose lichen Lobaria pulmonaria against photoinhibition
    Milos Barták
    Department of Plant Physiology and Anatomy, Masaryk University, Kotlarska 2, 61137, Brno, Czech Republic
    Oecologia 149:553-60. 2006
    This study aims to assess the photoprotective potential of desiccation-induced curling in the light-susceptible old forest lichen Lobaria pulmonaria by using chlorophyll fluorescence imaging...
  81. ncbi Analysis of desiccation-induced candidate phosphoproteins from Craterostigma plantagineum isolated with a modified metal oxide affinity chromatography procedure
    Horst Rohrig
    Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, Bonn, Germany
    Proteomics 8:3548-60. 2008
    ..indicates that this post-translational modification is also involved in the complex process of acquisition of desiccation tolerance...
  82. ncbi The effect of respiratory pattern on water loss in desiccation-resistant Drosophila melanogaster
    A E Williams
    Department of Ecology and Evolutionary Biology, University of California, Irvine 92697, USA
    J Exp Biol 201:2953-9. 1998
    ..release from individual fruit flies from five populations of Drosophila melanogaster selected for resistance to desiccation (D flies)...
  83. pmc Further optimization of mouse spermatozoa evaporative drying techniques
    Heidi Y Elmoazzen
    Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School and Shriners Hospital for Children, Boston, MA 02115, USA
    Cryobiology 59:113-5. 2009
    ..In previous desiccation work, dried sperm were stored in a vacuum-sealed plastic bag placed in a vacuum-packed Mylar bag...
  84. ncbi Dried blood spot HIV-1 RNA quantification using open real-time systems in South Africa and Burkina Faso
    Johannes Viljoen
    Africa Center for Health and Population Studies, University of KwaZulu Natal, Durban, South Africa
    J Acquir Immune Defic Syndr 55:290-8. 2010
    ..Our findings strongly advocate the implementation of DBS HIV-1 RNA testing in remote areas from low-income and middle-income countries...
  85. ncbi Slow desiccation leads to high-frequency shoot recovery from transformed somatic embryos of cotton (Gossypium hirsutum L. cv. Coker 310 FR)
    B Chaudhary
    Department of Genetics, University of Delhi South Campus, Benito Juarez Road, 110021 New Delhi, India
    Plant Cell Rep 21:955-60. 2003
    ..embryos were converted to plantlets was significantly improved by subjecting the embryos to slow physical desiccation. We used Agrobacterium strain GV3101 containing the binary vector pGSFR with the nos-nptII gene for in vitro ..
  86. pmc Long-term storage limits PCR-based analyses of malaria parasites in archival dried blood spots
    Joyce Hwang
    Shoklo Malaria Research Unit, Mae Sot, Tak Province, Thailand
    Malar J 11:339. 2012
    ..However, the suitability of the template obtained from dried blood spots that have been stored for long periods for DNA amplification is not known...
  87. pmc Desiccation tolerance of five tropical seedlings in panama. Relationship to a field assessment of drought performance
    Melvin T Tyree
    United States Department of Agriculture Forest Service, P O Box 968, Burlington, Vermont 05402, USA
    Plant Physiol 132:1439-47. 2003
    Studies of the desiccation tolerance of the seedlings of five tropical trees were made on potted plants growing in a greenhouse...
  88. ncbi Gefarnate stimulates secretion of mucin-like glycoproteins by corneal epithelium in vitro and protects corneal epithelium from desiccation in vivo
    M Nakamura
    Nara Research and Development Center, Santen Pharmaceutical Co, Ltd, Nara, Japan
    Exp Eye Res 65:569-74. 1997
    ..Corneal epithelial damage from desiccation was evaluated based on the extent of methylene blue staining...
  89. ncbi Glutathione half-cell reduction potential: a universal stress marker and modulator of programmed cell death?
    Ilse Kranner
    Seed Conservation Department, Royal Botanic Gardens, Kew, Wakehurst Place, West Sussex RH17 6TN, UK
    Free Radic Biol Med 40:2155-65. 2006
    ..We show in four species subjected to ageing or desiccation that seed viability decreased by 50% when the half-cell reduction potential of glutathione (E(GSSG/2GSH)), a ..
  90. ncbi MAPK phosphorylation is implicated in the adaptation to desiccation stress in nematodes
    Matthew C Banton
    Department of Chemical Engineering and Biotechnology, University of Cambridge, New Museums Site, Pembroke Street, Cambridge CB2 3RA, UK
    J Exp Biol 215:4288-98. 2012
    Some nematodes can survive almost complete desiccation by entering an ametabolic state called anhydrobiosis requiring the accumulation of protective molecules such as trehalose and LEA proteins...
  91. pmc Pleistocene desiccation in East Africa bottlenecked but did not extirpate the adaptive radiation of Lake Victoria haplochromine cichlid fishes
    Kathryn R Elmer
    Lehrstuhl fur Zoologie und Evolutionsbiologie, Department of Biology, University of Konstanz, 78457 Konstanz, Germany
    Proc Natl Acad Sci U S A 106:13404-9. 2009
    ..Paleolimnological evidence indicates dramatic desiccation of this lake ca. 18,000-15,000 years ago...
  92. pmc Ionizing-radiation resistance in the desiccation-tolerant cyanobacterium Chroococcidiopsis
    D Billi
    Department of Biological Science, Florida State University, Tallahassee, Florida 32306 1100, USA
    Appl Environ Microbiol 66:1489-92. 2000
    ..The radiation resistance of Chroococcidiopsis strains may reflect the ability of these cyanobacteria to survive prolonged desiccation through efficient repair of the DNA damage that accumulates during dehydration.
  93. pmc QTLs and candidate genes for desiccation and abscisic acid content in maize kernels
    Valérie Capelle
    Univ Paris Sud, Institut de Biotechnologie des Plantes, Bat 630, F 91405 Orsay, France
    BMC Plant Biol 10:2. 2010
    ..To provide some insight into the genetic determinism of kernel desiccation in maize, quantitative trait loci (QTLs) were detected for traits related to kernel moisture and ABA content in ..
  94. ncbi Increased corneal hydration induced by potential ocular penetration enhancers: assessment by differential scanning calorimetry (DSC) and by desiccation
    D Monti
    Department of Bioorganic Chemistry and Biopharmaceutics, University of Pisa, Via Bonanno 33, I 56126, Pisa, Italy
    Int J Pharm 232:139-47. 2002
    ..these agents; corneal hydration was then evaluated by measuring: (a) their total (free+bound) water content by desiccation (gravimetric analysis); and (b) their free water content by differential scanning calorimetry (DSC)...
  95. pmc Antioxidants and photoprotection in a lichen as compared with its isolated symbiotic partners
    Ilse Kranner
    Institute of Plant Sciences, Karl Franzens University of Graz, Schubertstrasse 51, A 8010 Graz, Austria
    Proc Natl Acad Sci U S A 102:3141-6. 2005
    Extreme desiccation and irradiation increase the formation of reactive oxygen species in organisms...
  96. ncbi The LEA-like protein HSP 12 in Saccharomyces cerevisiae has a plasma membrane location and protects membranes against desiccation and ethanol-induced stress
    K Sales
    Department of Biochemistry, University of Cape Town, Private Bag, Rondebosch, South Africa
    Biochim Biophys Acta 1463:267-78. 2000
    ..encapsulating the fluorescent dye calcein was used to investigate the protection by HSP 12 of membranes during desiccation. HSP 12 was found to act in an analogous manner to trehalose and protect liposomal membrane integrity against ..
  97. pmc Global response to desiccation stress in the soil actinomycete Rhodococcus jostii RHA1
    Justin C LeBlanc
    Department of Microbiology and Immunology, Life Sciences Institute, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, Canada
    Appl Environ Microbiol 74:2627-36. 2008
    ..Here we report the first transcriptomic analysis of a gram-positive bacterium during desiccation. Filtered RHA1 cells incubated at either low relative humidity (20%), as an air-drying treatment, or high ..
  98. ncbi The desert of Tataouine: an extreme environment that hosts a wide diversity of microorganisms and radiotolerant bacteria
    Angélique Chanal
    LEMIR, DEVM, DSV, UMR 6191 CNRS CEA Université Aix Marseille II, IFR 112, CEA Cadarache, F 13108 Saint Paul lez Durance, France
    Environ Microbiol 8:514-25. 2006
    ..A correlation between tolerance to desiccation and to radiation has been demonstrated for the radiotolerant bacteria Deinococcus radiodurans...
  99. pmc Hydroxyectoine is superior to trehalose for anhydrobiotic engineering of Pseudomonas putida KT2440
    M Manzanera
    Institute of Biotechnology, University of Cambridge, Cambridge CB2 1QT, United Kingdom
    Appl Environ Microbiol 68:4328-33. 2002
    Anhydrobiotic engineering aims to increase the level of desiccation tolerance in sensitive organisms to that observed in true anhydrobiotes...
  100. pmc Evaluation of different RNA extraction methods and storage conditions of dried plasma or blood spots for human immunodeficiency virus type 1 RNA quantification and PCR amplification for drug resistance testing
    Marjorie Monleau
    UMR 145, Institut de Recherche pour le Developpement, and University of Montpellier 1, Montpellier, France
    J Clin Microbiol 47:1107-18. 2009
    ..DBS could be used as an alternative for DPS depending on HIV RNA cutoffs for virological failure. VL measurements remain stable over a longer period than do PCR amplification results...
  101. ncbi Conversion of nanosuspensions into dry powders by spray drying: a case study
    Mahesh V Chaubal
    Baxter Healthcare Corporation, Route 120 and Wilson Rd, Round Lake, Illinois 60073, USA
    Pharm Res 25:2302-8. 2008
    ..This case study examines impact of various formulation and processing parameters on redispersibility of the spray dried nanoparticles...

Research Grants62

  1. Novel Bacillus subtilis based spore vaccine to tuberculosis
    Subramanian Dhandayuthapani; Fiscal Year: 2013
    ..spores are dormant and rugged, and maintain their integrity under extreme conditions, including low pH, desiccation, UV irradiation and temperatures of up to 90oC...
  2. Interactions between Bacterial Tyrosine Kinases and Phosphatases
    Ranajeet Ghose; Fiscal Year: 2013
    ..be ascertained by assaying cells expressing mutant proteins for polysaccharide synthesis and survival under desiccation. We expect that our present studies, limited to the E...
  3. Regulatory Circuits Controlling Development of Dormant Microbial Cysts
    CARL EUGENE BAUER; Fiscal Year: 2013
    ..Cysts are metabolically dormant resting cells that are extremely resistant to desiccation as well as to growth inhibitors such as to antibiotics...
  4. Polyamine-dependent regulation of Vibrio cholerae biofilm formation
    Ece Karatan; Fiscal Year: 2011
    ..Biofilm bacteria are more tolerant to environmental stresses such as UV radiation, desiccation, low pH, antimicrobials, and the immune defenses of the human host. This tolerance protects V...
  5. Sex, sex steroids and dry eye syndromes
    David A Sullivan; Fiscal Year: 2013
    ..Tear film dysfunction, in turn, may severely impact the eye and lead to desiccation, ulceration and perforation of the cornea, an increased incidence of infectious disease, and pronounced visual ..
  6. Peptidoglycan synthesis during sporulation
    Jonathan Dworkin; Fiscal Year: 2012
    ..contains a thick layer of peptidoglycan, the spore cortex, which is largely responsible for its heat and desiccation resistance...
  7. Drying, Storing and Reanimating Egg Germinal Vesicles to Preserve Fertility
    Pierre Comizzoli; Fiscal Year: 2013
    ..The general hypothesis is that desiccation and storage at room temperature can preserve the structural and functional integrity of the germinal vesicle...
  8. Functional Analysis and Systems Biology of Filamentous Fungi
    Jay C Dunlap; Fiscal Year: 2013
    ..We will focus on two key triggers of asexual development: light and desiccation. In addition, we will leverage our prior successes to expand systematic knockouts to an additional prominent ..
  9. Identification of bacteria-associated cues that regulate hatching of Aedes aegypt
    Loganathan Ponnusamy; Fiscal Year: 2013
    ..Aedes aegypti is a peri-domestic species that lays desiccation- resistant eggs in water-filled human-made containers...
  10. Prolactin as a lacrimal gland immunomodulator
    Joel Schechter; Fiscal Year: 2009
    ..Specific Aim 4 tests the hypothesis that homeostatic states associated with certain endocrine settings cause desiccation or trauma at the ocular surface to elicit exaggerated inflammatory responses
  11. Regulation of akinete differentiation in Nostoc punctiforme
    Michael L Summers; Fiscal Year: 2013
    ..Akinetes can withstand environmental stress, such as desiccation and cold that kill normal vegetative cells within the filament...
  12. Interrogation of systems level mechanisms controlling DNA repair processes
    Nitin S Baliga; Fiscal Year: 2010
    ..This halophilic archaeon routinely mitigates damage from high salinity, UV radiation and desiccation-rehydration cycles...
  13. A Pragmatic Trial of Lumbar Image Reporting with Epidemiology (LIRE)
    Jeffrey G Jarvik; Fiscal Year: 2012
    ..without back pain, magnetic resonance (MR) imaging of the lumbar spine frequently reveals findings such as disc desiccation or bulging...
  14. Cuticular proteins of Anopheles gambiae
    Judith Willis; Fiscal Year: 2013
    ..Other laboratories have implicated the cuticular proteins of Anopheles in insecticide and desiccation resistance, in mate recognition, and in being synthesized in response to a blood meal...
  15. Toll-Like Receptor and Dry Eye Inflammation and Infection
    RACHEL LEIGH REDFERN; Fiscal Year: 2013
    ..colonization via the production of antimicrobial peptides (AMPs) when the ocular surface is compromised from desiccation. This scenario is consistent with the clinical presentation of DES, as concomitant microbial infections are ..
  16. Seasonal Adaptation of a Circadian Clock
    Isaac Edery; Fiscal Year: 2013
    ..to decreases in per RNA levels, events that prolong midday siesta and likely minimize the risks associated with desiccation during the hot midday hours. Recent progress indicates that multiple suboptimal splicing signals [i.e...
  17. Seasonal Adaptation of a Circadian Clock
    Isaac Edery; Fiscal Year: 2009
    ..accumulation of per RNA levels, events that enhance midday siesta and likely minimize the risks associated with desiccation during the hot midday hours. Recent progress indicates that multiple suboptimal splicing signals [i.e...
  18. Mathematical Modeling of Cell Biopreservation by Desiccation
    STEPHEN HOWARD DAVIS; Fiscal Year: 2011
    ..mathematical investigation of the key processes governing biopreservation of cells by vitrification and desiccation. The research will be focused on the formation of a glassy state in biological solutions and the interaction of ..
  19. Biochemistry of Chorion Hardening in Mosquitoes
    Jianyong Li; Fiscal Year: 2005
    ..Therefore, the ability to resist desiccation and other adverse environmental conditions is critical for the survival of these eggs...
  20. Preserving Platelets for storage and transportation using a novel desiccation pro
    David H Ho; Fiscal Year: 2012
    ..technology involves cell specific dehydration and rehydration buffering reagents coupled with vacuum assisted desiccation. A documented protocol for erythrocytes has been established and dried red cells are available in limited ..
  21. Funtion of Uncharacterized Genes of Acinetobacter baumanii
    Samuel I Miller; Fiscal Year: 2013
    ..use expression analysis to identify genes and sRNAs of unknown function associated with drug, disinfectant, and desiccation tolerance (Carrie Harwood, Project 3 Director)...
  22. EXERCISE--EFFECTS ON ETOH IMPAIRMENT OF BEHAVIOR IN MICE
    SANDRA MOLLENAUER; Fiscal Year: 1993
    ..will be obtained by assessing effects of prior exercise on 1) body composition (body water determined by desiccation), 2) blood EtOH concentration (BEC) at regaining of the righting response (RORR), and 3) EtOH clearance rate ..
  23. REGULATION OF SYNTHESIS OF A HORMONE INDUCED PROTEIN
    GLENN GALAU; Fiscal Year: 1991
    ..include the plant hormone abscisic acid and probably events related to incipient dehydration prior to full desiccation of the mature seed...
  24. Perpetuation of Francisella tularensis
    Sam R Telford; Fiscal Year: 2010
    ..Taken together, these observations will provide a basis for understanding how the agent of tularemia is perpetuated on Martha's Vineyard, and why pneumonic human disease is prevalent there. ..
  25. STRUCTURE AND FUNCTION OF HUMAN SALIVARY MUCINS
    Gwynneth Offner; Fiscal Year: 2009
    ..MG2 appears to contain a single mucin gene product, MUC7. Mucins protect oral surfaces against desiccation, lubricate hard and soft tissues to facilitate mastication, speech and swallowing and form a selectively ..
  26. Identification of T Cell Antigen For Q Fever Vaccination
    James Samuel; Fiscal Year: 2009
    ..Human infection arises primarily from aerosol transmission and C. burnetii can withstand desiccation and remain infectious in contaminated soils for several years...
  27. TREHALOSE METABOLISM IN METHYLOTROPHIC YEAST
    EDWARD BELLION; Fiscal Year: 1993
    ..In bakers' yeast it is synthesized in response to starvation and heat shock and also protects against desiccation, whereas in bacteria it plays critical role as an osmoprotectant, and is rapidly synthesized when cells are ..
  28. NEUROENDOCRINE REGULATION OF WATER BALANCE IN INSECTS
    JEFFREY SPRING; Fiscal Year: 1991
    ..Because of their small size and large surface area, insects are extremely vulnerable to desiccation and studies of the endocrine control of excretion may, therefore, lead the way towards development of very ..
  29. CHARACTERIZATION OF B ANTHRACIS EXOSPORIUM PROTEINS
    CHARLES TURNBOUGH; Fiscal Year: 2004
    ..The spore's resistance to extreme temperatures, noxious chemicals, desiccation, and physical damage make it suitable for incorporation into explosive weapons and for concealment in terrorist ..
  30. Preservation of Embryonic Stem Cells in Desiccated State
    Mehmet Toner; Fiscal Year: 2007
    ..Although cell stasis is routinely achieved in nature by anhydrobiotic organisms through desiccation at ambient temperatures, the only existing strategy for long term storage of mammalian cells is ..
  31. WHOLE GENOME SEQUENCING OF COXIELLA BURNETTI
    John Heidelberg; Fiscal Year: 2000
    ..Additionally, the genome will be annotated by computer techniques to identify all open reading frames (ORFs) and relate as many ORFs as possible to proteins of known function. ..
  32. High and Low Frequency Scanning Acoustic Microscopes
    Paulette Spencer; Fiscal Year: 2002
    ..environment reduces the potential for introducing artifacts in those biological specimens that are sensitive to desiccation. The high and low frequency SAM would be a shared resource and a cadre of 5 NIH-funded scientists have been ..
  33. EVOLUTION OF DURABILITY IN EMERGING BACTERIAL PATHOGENS
    THOMAS WHITTAM; Fiscal Year: 2000
    ..2) to investigate the evolution of the components of durability (acid, salt, and heat tolerance, resistance to desiccation) and their correlated change with selection for single resistance factors; (3) to quantify variation in ..
  34. Cellular Engineering for Metabolic Stasis
    Mehmet Toner; Fiscal Year: 2007
    ..many animals and organisms down regulate their metabolism and may enter into a state of stasis by either desiccation through removal of water from their cells (i.e...
  35. ROLE OF IRON-OXO CLUSTERS IN FATTY ACID DESATURATION
    Brian Fox; Fiscal Year: 2007
    ..The potential of using related molecules to inactivate other position-specific desaturases will be determined. ..
  36. The Contact Lens and Dry Eye Study
    Jason Nichols; Fiscal Year: 2006
    ..Contact lens-related dry eye may severely impact ocular health by leading to desiccation of the corneal epithelium or an increased incidence of infectious disease...
  37. AN IMPROVED WOUND DRESSING SUITABLE FOR BURN TREATMENT
    MARK DILLON; Fiscal Year: 1992
    ..The same dressing will decrease its vapor permeability when exudate decreases, thus avoiding wound desiccation. This Phase I project will begin with prototype production, continue with in vitro and in vivo testing, and ..
  38. GENETICALLY POSTPONED SENESCENCE IN DROSOPHILA
    Michael Rose; Fiscal Year: 1991
    ..done by artificial selection on characters which enhance adult survival, specifically resistance to starvation, desiccation, and low-levels of ambient ethanol...
  39. SUSTAINED GENE TRANSFER FOR CHRONIC WOUND HEALING
    Weiliam Chen; Fiscal Year: 2000
    ..of chronic topical wounds requires multiple interventions and thus predisposes patients to discomforts, desiccation and infection which are detrimental to wound healing...
  40. Physiological response of Escherichia coli to temperature and desiccation stress
    SUSAN BORNSTEINFORST; Fiscal Year: 2008
    ..2 investigates the induction and modulation of two physiological responses that may confer protection against desiccation stress in sand; namely trehalose synthesis and shifts in porin protein expression...
  41. ANIMAL MODELS OF COLD AIR-INDUCED AIRWAY DISEASE
    Michael Davis; Fiscal Year: 2004
    Inspired air is conditioned (warmed and humidified) by the upper airways to prevent cooling and desiccation of the lung parenchyma...
  42. NCRR Training Grant for Veterinary Students in Research
    Michael Davis; Fiscal Year: 2006
    ..Over a three-year period this program will contribute nine comparative medical scientists with veterinary medical degrees who are well trained in animal-oriented, hypothesis-based research. [unreadable] [unreadable]..
  43. Veterinary Comparative Respiratory Society 2008 Symposium
    Michael Davis; Fiscal Year: 2008
    ..This symposium will review the current knowledge and practical challenges of these models in order to make them more accessible to the biomedical research community. [unreadable] [unreadable] [unreadable]..
  44. Determinants of the Durability of Resin-Dentin Bonds
    David Pashley; Fiscal Year: 2006
    ..This should provide important new insight into designing more durable resin-dentin bonds that can justify the placement of resin-based posterior composites. ..
  45. CONTROLLED-RELEASE SEALANT TO PREVENT SECONDARY CARIES
    Kenneth Anusavice; Fiscal Year: 2004
    ..mutans, site specific levels of S. mutans at the margins of amalgam and composite restorations, and the width of marginal crevices adjacent to amalgam and composite restorations). ..
  46. Epigenetic Regulation of Gene Expression
    Robert Martienssen; Fiscal Year: 2003
    ..It is in a cross-disciplinary environment such as this that intellectual leaps occur and innovative ideas flourish. ..
  47. Library-based selection of optimal gene inhibitors
    SERGEI KAZAKOV; Fiscal Year: 2003
    ..In this way, optimized Lassos will be derived in an automatic and straightforward manner for application as agents for target validation, gene function analysis, and therapeutics. ..
  48. Amplification of Custom RNA in Plants
    SERGEI KAZAKOV; Fiscal Year: 2002
    ..Our current business strategy is to sell custom RNA in partnership with Large Scale Biology Corp. and license the technology to certain other manufacturers. ..
  49. SPECIES BOUNDARIES IN THE ANOPHELES GAMBIAE COMPLEX
    Nora Besansky; Fiscal Year: 2003
    ..gambiae and An. arabiensis sister taxa? (2) How much gene flow is occurring between them, and which genomic regions are susceptible? (3) How much gene flow is occurring between chromosomal forms of An. gambiae? ..
  50. CRYOPRESERVATION OF ARTICULAR CARTILAGE BY VITRIFICATION
    Kelvin Brockbank; Fiscal Year: 2003
    ..The U.S. market for cartilage repair products is predicted to exceed 90,000 units and $248 million in the near future. ..
  51. Genetics of Anopheles funestus populations
    Nora Besansky; Fiscal Year: 2005
    ..funestus become possible ..
  52. Safety Pharmacokinetics & Efficacy of Artemether & Lumefantrine in Pregnant Women
    Mark Mirochnick; Fiscal Year: 2008
    ..The data from this planned study will provide the information needed to ensure that pregnant women receive the appropriate doses of these anti-malaria drugs. [unreadable] [unreadable] [unreadable]..
  53. Contrasting environmental and genetic controls of alternative phenotypes
    JENNIFER BRISSON; Fiscal Year: 2008
    ..This project aims to illuminate basic rules acting to integrate environmental and genetic signals in the production of the phenotype. [unreadable] [unreadable] [unreadable]..
  54. Elevated potassium ion in lacrimal fluid and the health of the ocular surface
    John Ubels; Fiscal Year: 2009
    ..If the high potassium in tears is important for the health of the ocular surface, this information will be useful in the development of improved treatments for dry eye disease. ..
  55. Lactate Transport as a Therapeutic Target in Glioma
    SAROJ PRIYANTHA MATHUPALA; Fiscal Year: 2010
    ..Thus, we need new ways to target this deadly cancer. We plan to test a novel method to "pickle-the-tumor-to-death," while leaving healthy brain cells intact. ..
  56. The effect of oxidative stress on muscle damage and functional senesence.
    Jason Williams; Fiscal Year: 2008
    ..This research will provide insight into muscle aging in humans and mechanisms underlying muscular diseases associated with oxidative stress. [unreadable] [unreadable] [unreadable]..
  57. Topical Antibiotics to Prevent Lyme Disease
    Gary Wormser; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  58. Antiretroviral Pharmacology/Lactating Mother/Infants
    Mark Mirochnick; Fiscal Year: 2006
    ..This information is needed for the safe and effective use of antiretrovirals in nursing women, and will pioneer a novel approach to the study of breast milk drug transfer. ..
  59. Methylmercury toxicity in neural development
    MATTHEW RAND; Fiscal Year: 2006
    ..This information will help direct strategies for prevention of MeHg toxicity, for example through pharmacologic administration of protease inhibitors. ..
  60. A new method for multiplex detection of micro-RNAs (miR-ID)
    SERGEI KAZAKOV; Fiscal Year: 2008
    ..The method of micro-RNA detection has novel features that promise better sensitivity and selectivity than other micro-RNA assays currently available. [unreadable] [unreadable] [unreadable] [unreadable]..