x ray crystallography

Summary

Summary: The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

Top Publications

  1. ncbi Coot: model-building tools for molecular graphics
    Paul Emsley
    York Structural Biology Laboratory, University of York, Heslington, York YO10 5YW, England
    Acta Crystallogr D Biol Crystallogr 60:2126-32. 2004
  2. ncbi Inference of macromolecular assemblies from crystalline state
    Evgeny Krissinel
    European Bioinformatics Institute, Genome Campus, Hinxton, Cambridge CB10 1SD, UK
    J Mol Biol 372:774-97. 2007
  3. ncbi Crystal structure of the nucleosome core particle at 2.8 A resolution
    K Luger
    Institut fur Molekularbiologie und Biophysik, Zurich, Switzerland
    Nature 389:251-60. 1997
  4. ncbi Optimal description of a protein structure in terms of multiple groups undergoing TLS motion
    Jay Painter
    Biomolecular Structure Center, Department of Biochemistry, University of Washington, Seattle, WA 98195 7742, USA
    Acta Crystallogr D Biol Crystallogr 62:439-50. 2006
  5. ncbi Refinement of severely incomplete structures with maximum likelihood in BUSTER-TNT
    E Blanc
    Global Phasing Ltd, Sheraton House, Castle Park, Cambridge CB3 0AX, England
    Acta Crystallogr D Biol Crystallogr 60:2210-21. 2004
  6. ncbi GPCR engineering yields high-resolution structural insights into beta2-adrenergic receptor function
    Daniel M Rosenbaum
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Science 318:1266-73. 2007
  7. ncbi Scaling and assessment of data quality
    Philip Evans
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, England
    Acta Crystallogr D Biol Crystallogr 62:72-82. 2006
  8. ncbi Crystal structure of rhodopsin: A G protein-coupled receptor
    K Palczewski
    Department of Ophthalmology, University of Washington, Seattle, WA 98195, USA
    Science 289:739-45. 2000
  9. pmc Toward the structural genomics of complexes: crystal structure of a PE/PPE protein complex from Mycobacterium tuberculosis
    Michael Strong
    Howard Hughes Medical Institute, UCLA Department of Energy Institute of Genomics and Proteomics, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 103:8060-5. 2006
  10. ncbi Design of a novel globular protein fold with atomic-level accuracy
    Brian Kuhlman
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Science 302:1364-8. 2003

Detail Information

Publications375 found, 100 shown here

  1. ncbi Coot: model-building tools for molecular graphics
    Paul Emsley
    York Structural Biology Laboratory, University of York, Heslington, York YO10 5YW, England
    Acta Crystallogr D Biol Crystallogr 60:2126-32. 2004
    ..The map-fitting tools are available as a stand-alone package, distributed as 'Coot'...
  2. ncbi Inference of macromolecular assemblies from crystalline state
    Evgeny Krissinel
    European Bioinformatics Institute, Genome Campus, Hinxton, Cambridge CB10 1SD, UK
    J Mol Biol 372:774-97. 2007
    ..The method is implemented as a public WWW service...
  3. ncbi Crystal structure of the nucleosome core particle at 2.8 A resolution
    K Luger
    Institut fur Molekularbiologie und Biophysik, Zurich, Switzerland
    Nature 389:251-60. 1997
    ..The lack of uniformity between multiple histone/DNA-binding sites causes the DNA to deviate from ideal superhelix geometry...
  4. ncbi Optimal description of a protein structure in terms of multiple groups undergoing TLS motion
    Jay Painter
    Biomolecular Structure Center, Department of Biochemistry, University of Washington, Seattle, WA 98195 7742, USA
    Acta Crystallogr D Biol Crystallogr 62:439-50. 2006
    ..The models generated by TLSMD analysis can significantly improve the standard crystallographic residuals R and R(free) and can reveal intrinsic dynamic properties of the protein...
  5. ncbi Refinement of severely incomplete structures with maximum likelihood in BUSTER-TNT
    E Blanc
    Global Phasing Ltd, Sheraton House, Castle Park, Cambridge CB3 0AX, England
    Acta Crystallogr D Biol Crystallogr 60:2210-21. 2004
    ....
  6. ncbi GPCR engineering yields high-resolution structural insights into beta2-adrenergic receptor function
    Daniel M Rosenbaum
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Science 318:1266-73. 2007
    ..Amino acids known to regulate receptor function are linked through packing interactions and a network of hydrogen bonds, suggesting a conformational pathway from the ligand-binding pocket to regions that interact with G proteins...
  7. ncbi Scaling and assessment of data quality
    Philip Evans
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, England
    Acta Crystallogr D Biol Crystallogr 62:72-82. 2006
    ..These scoring functions are implemented in a new program POINTLESS...
  8. ncbi Crystal structure of rhodopsin: A G protein-coupled receptor
    K Palczewski
    Department of Ophthalmology, University of Washington, Seattle, WA 98195, USA
    Science 289:739-45. 2000
    ..Identification of a set of residues that mediate interactions between the transmembrane helices and the cytoplasmic surface, where G-protein activation occurs, also suggests a possible structural change upon photoactivation...
  9. pmc Toward the structural genomics of complexes: crystal structure of a PE/PPE protein complex from Mycobacterium tuberculosis
    Michael Strong
    Howard Hughes Medical Institute, UCLA Department of Energy Institute of Genomics and Proteomics, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 103:8060-5. 2006
    ..Our entire procedure for the identification, characterization, and structural determination of protein complexes can be scaled to a genome-wide level...
  10. ncbi Design of a novel globular protein fold with atomic-level accuracy
    Brian Kuhlman
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Science 302:1364-8. 2003
    ..2 angstroms) to the design model. The ability to design a new protein fold makes possible the exploration of the large regions of the protein universe not yet observed in nature...
  11. ncbi An allosteric mechanism for activation of the kinase domain of epidermal growth factor receptor
    Xuewu Zhang
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, 94720, USA
    Cell 125:1137-49. 2006
    ..The CDK/cyclin-like complex formed by two kinase domains thus explains the activation of EGFR-family receptors by homo- or heterodimerization...
  12. pmc Maximum-likelihood density modification
    T C Terwilliger
    Structural Biology Group, Mail Stop M888, Los Alamos National Laboratory, Los Alamos, NM 87545, USA
    Acta Crystallogr D Biol Crystallogr 56:965-72. 2000
    ..d55, 1863-1871]...
  13. pmc High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor
    Vadim Cherezov
    Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    Science 318:1258-65. 2007
    ....
  14. ncbi Structural characterization of flexible proteins using small-angle X-ray scattering
    Pau Bernado
    European Molecular Biology Laboratory, Hamburg Outstation, 22603 Hamburg, Germany
    J Am Chem Soc 129:5656-64. 2007
    ..In the latter case, EOM is able to distinguish between rigid and flexible proteins and to directly assess the interdomain contacts...
  15. ncbi Crystal structure of a mammalian voltage-dependent Shaker family K+ channel
    Stephen B Long
    Howard Hughes Medical Institute, Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 309:897-903. 2005
    ..Electrostatic properties of the side portals and positions of the T1 domain and beta subunit are consistent with electrophysiological studies of inactivation gating and with the possibility of K+ channel regulation by the beta subunit...
  16. doi Crystal structure of the ligand-free G-protein-coupled receptor opsin
    Jung Hee Park
    Institut für Medizinische Physik und Biophysik CC2, Charite Universitatsmedizin Berlin, Chariteplatz 1, D 10117 Berlin, Germany
    Nature 454:183-7. 2008
    ..The opsin structure sheds new light on ligand binding to GPCRs and on GPCR activation...
  17. doi Automated structure solution with the PHENIX suite
    Peter H Zwart
    Lawrence Berkeley National Laboratory, Berkeley, CA, USA
    Methods Mol Biol 426:419-35. 2008
    ..The algorithms are tightly linked and made easily accessible to users through the PHENIX Wizards and the PHENIX GUI...
  18. ncbi Structure of staphylococcal alpha-hemolysin, a heptameric transmembrane pore
    L Song
    Department of Biochemistry, University of Chicago, 920 East 58 Street, Chicago, IL 60637, USA
    Science 274:1859-66. 1996
    ....
  19. ncbi The structure of the potassium channel: molecular basis of K+ conduction and selectivity
    D A Doyle
    Laboratory of Molecular Neurobiology and Biophysics and the Howard Hughes Medical Institute, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 280:69-77. 1998
    ..The architecture of the pore establishes the physical principles underlying selective K+ conduction...
  20. ncbi Structure of the 70S ribosome complexed with mRNA and tRNA
    Maria Selmer
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Science 313:1935-42. 2006
    ..The interactions of E-site tRNA with the 50S subunit have both similarities and differences compared to those in the archaeal ribosome. The structure also rationalizes much biochemical and genetic data on translation...
  21. ncbi X-ray structure of a protein-conducting channel
    Bert van den Berg
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 427:36-44. 2004
    ....
  22. pmc Structure of a beta1-adrenergic G-protein-coupled receptor
    Tony Warne
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Nature 454:486-91. 2008
    ....
  23. ncbi Crystal structure of the lactose operon repressor and its complexes with DNA and inducer
    M Lewis
    Johnson Research Foundation, University of Pennsylvania, Philadelphia 19104, USA
    Science 271:1247-54. 1996
    ....
  24. ncbi Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins
    Toshimitsu Kawate
    Department of Biochemistry and Molecular Biophysics, Columbia University, 650 West 168 th Street, New York, New York 10032, USA
    Structure 14:673-81. 2006
    ..We show the application of this precrystallization screening to four membrane proteins derived from prokaryotic or eukaryotic organisms...
  25. ncbi Crystal structure of the extracellular segment of integrin alpha Vbeta3 in complex with an Arg-Gly-Asp ligand
    Jian Ping Xiong
    Renal Unit, Leukocyte Biology and Inflammation Program, Structural Biology Program, Massachusetts General Hospital, 149 13th Street, Charlestown, MA 02129, USA
    Science 296:151-5. 2002
    ..Ligand binding induces small changes in the orientation of alphaV relative to beta3...
  26. pmc BALBES: a molecular-replacement pipeline
    Fei Long
    York Structural Biology Laboratory, Chemistry Department, University of York, Heslington, York, England
    Acta Crystallogr D Biol Crystallogr 64:125-32. 2008
    ..Testing the current version of the pipeline using entries from the PDB has shown that this approach has huge potential and that around 75% of structures can be solved automatically without user intervention...
  27. pmc Automated macromolecular model building for X-ray crystallography using ARP/wARP version 7
    Gerrit Langer
    European Molecular Biology Laboratory, c o DESY, Notkestrasse 85, Hamburg 22607, Germany
    Nat Protoc 3:1171-9. 2008
    ..All protocols are easy to handle by a nonexpert user through a graphical user interface or a command line. The time required is typically a few minutes although iterative model building may take a few hours...
  28. ncbi Crystallography & NMR system: A new software suite for macromolecular structure determination
    A T Brunger
    The Howard Hughes Medical Institute, Yale University, New Haven, CT 06511, USA, and Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511, USA
    Acta Crystallogr D Biol Crystallogr 54:905-21. 1998
    ..User-friendly task-oriented input files are available for nearly all aspects of macromolecular structure determination by X-ray crystallography and solution NMR...
  29. ncbi Crystal structure of the Src family tyrosine kinase Hck
    F Sicheri
    Laboratories of Molecular Biophysics, The Rockefeller University, New York 10021, USA
    Nature 385:602-9. 1997
    ..The conformation of the active site has similarities with that of inactive cyclin-dependent protein kinases...
  30. ncbi HKL-3000: the integration of data reduction and structure solution--from diffraction images to an initial model in minutes
    Wladek Minor
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22903, USA
    Acta Crystallogr D Biol Crystallogr 62:859-66. 2006
    ..The current development is oriented towards very fast structure solution in order to provide feedback during the diffraction experiment. Work is also proceeding towards improving the quality of phasing calculation and model building...
  31. ncbi Mechanism of CDK activation revealed by the structure of a cyclinA-CDK2 complex
    P D Jeffrey
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 376:313-20. 1995
    ..These changes activate the kinase by realigning active site residues and relieving the steric blockade at the entrance of the catalytic cleft...
  32. ncbi Structure of the dengue virus envelope protein after membrane fusion
    Yorgo Modis
    Howard Hughes Medical Institute, Children s Hospital and Harvard Medical School, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 427:313-9. 2004
    ..Specific features of the folded-back structure suggest strategies for inhibiting flavivirus entry...
  33. ncbi Crystal structure of bacterial multidrug efflux transporter AcrB
    Satoshi Murakami
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567 0047, Japan
    Nature 419:587-93. 2002
    ....
  34. ncbi The Ras-RasGAP complex: structural basis for GTPase activation and its loss in oncogenic Ras mutants
    K Scheffzek
    Max Planck Institut fur molekulare Physiologie, Abteilung Strukturelle Biologie, Rheinlanddamm 201, 44139 Dortmund, Germany
    Science 277:333-8. 1997
    ..Glycine-12 in the transition state mimic is within van der Waals distance of both arginine-789 of GAP-334 and glutamine-61 of Ras, and even its mutation to alanine would disturb the arrangements of residues in the transition state...
  35. ncbi Structure of 20S proteasome from yeast at 2.4 A resolution
    M Groll
    Max Planck Institut fur Biochemie, Martinsreid, Germany
    Nature 386:463-71. 1997
    ....
  36. ncbi Crystal structure of an Hsp90-nucleotide-p23/Sba1 closed chaperone complex
    Maruf M U Ali
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Nature 440:1013-7. 2006
    ..Contrary to expectations, the closed Hsp90 would not enclose its client proteins but provides a bipartite binding surface whose formation and disruption are coupled to the chaperone ATPase cycle...
  37. ncbi Evaluating protein structures determined by structural genomics consortia
    Aneerban Bhattacharya
    Center for Advanced Biotechnology and Medicine, Northeast Structural Genomics Consortium, Rutgers University and Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Proteins 66:778-95. 2007
    ..Potential reasons for this "structure quality score gap" between NMR and X-ray crystal structures are discussed...
  38. pmc Structural basis for recruitment of human flap endonuclease 1 to PCNA
    Shigeru Sakurai
    Structural Biology Laboratory, Nara Institute of Science and Technology, Takayama, Ikoma, Nara, Japan
    EMBO J 24:683-93. 2005
    ..A hinge region present between the core domain and the C-terminal tail of FEN1 would play a role in switching the FEN1 orientation from an inactive to an active orientation...
  39. doi Global twisting motion of single molecular KcsA potassium channel upon gating
    Hirofumi Shimizu
    CREST Sasaki team, Japan Science and Technology Corporation, Tachikawa, Tokyo, 190 0012, Japan
    Cell 132:67-78. 2008
    ..This coupling suggests a mechanical interplay between the transmembrane and cytoplasmic domains...
  40. doi Crystal structure of opsin in its G-protein-interacting conformation
    Patrick Scheerer
    Institut für Medizinische Physik und Biophysik CC2, Charite Universitatsmedizin Berlin, Chariteplatz 1, D 10117 Berlin, Germany
    Nature 455:497-502. 2008
    ..On the basis of the Ops*-GalphaCT structure and known conformational changes in Galpha, we discuss signal transfer from the receptor to the G protein nucleotide-binding site...
  41. pmc ElNemo: a normal mode web server for protein movement analysis and the generation of templates for molecular replacement
    Karsten Suhre
    Information Génomique and Structurale UPR CNRS 2589, 31, Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Nucleic Acids Res 32:W610-4. 2004
    ..The web server can be freely accessed at http://igs-server.cnrs-mrs.fr/elnemo/index.html...
  42. ncbi The Buccaneer software for automated model building. 1. Tracing protein chains
    Kevin Cowtan
    Department of Chemistry, University of York, Heslington, York YO10 5DD, England
    Acta Crystallogr D Biol Crystallogr 62:1002-11. 2006
    ..Techniques for assembling the chain fragments into an initial chain trace are discussed...
  43. ncbi Structure at 2.8 A resolution of F1-ATPase from bovine heart mitochondria
    J P Abrahams
    Medical Research Council Laboratory of Molecular Biology, Cambridge, UK
    Nature 370:621-8. 1994
    ..Interconversion of the states may be achieved by rotation of the alpha 3 beta 3 subassembly relative to an alpha-helical domain of the gamma-subunit...
  44. ncbi Three-dimensional structure of the tyrosine kinase c-Src
    W Xu
    Laboratory of Molecular Medicine, Children s Hospital, Boston, Massachusetts 02115, USA
    Nature 385:595-602. 1997
    ..The structure shows how appropriate cellular signals, or transforming mutations in v-Src, could break these interactions to produce an open, active kinase...
  45. ncbi Crystal structure of Argonaute and its implications for RISC slicer activity
    Ji Joon Song
    Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Science 305:1434-7. 2004
    ..The architecture of the molecule and the placement of the PAZ and PIWI domains define a groove for substrate binding and suggest a mechanism for siRNA-guided mRNA cleavage...
  46. ncbi The structure of a human type III Fcgamma receptor in complex with Fc
    S Radaev
    Structural Biology Section, Laboratory of Immunogenetics, NIAID, National Institutes of Health, Rockville, Maryland 20852, USA
    J Biol Chem 276:16469-77. 2001
    ..An antibody-FcgammaRIII model suggests two possible ligand-induced receptor aggregations...
  47. ncbi X-ray structure of the FimC-FimH chaperone-adhesin complex from uropathogenic Escherichia coli
    D Choudhury
    Department of Molecular Biology, Uppsala Biomedical Center, Swedish University of Agricultural Sciences, Box 590, S 753 24 Uppsala, Sweden
    Science 285:1061-6. 1999
    ..A donor strand complementation mechanism in which the chaperone donates a strand to complete the pilin domain explains the basis for both chaperone function and pilus biogenesis...
  48. pmc A pharmacological map of the PI3-K family defines a role for p110alpha in insulin signaling
    Zachary A Knight
    Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, CA 94143, USA
    Cell 125:733-47. 2006
    ..These results illustrate systematic target validation using a matrix of inhibitors that span a protein family...
  49. ncbi Crystal structure of the human beta2 adrenergic G-protein-coupled receptor
    Søren G F Rasmussen
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, 279 Campus Drive, Stanford, Palo Alto, California 94305, USA
    Nature 450:383-7. 2007
    ..These differences may be responsible for the relatively high basal activity and structural instability of the beta2AR, and contribute to the challenges in obtaining diffraction-quality crystals of non-rhodopsin GPCRs...
  50. ncbi DNA-bound structures and mutants reveal abasic DNA binding by APE1 and DNA repair coordination [corrected]
    C D Mol
    Skaggs Institute for Chemical Biology, and the Department of Molecular Biology, La Jolla, California 92037 1027, USA
    Nature 403:451-6. 2000
    ....
  51. ncbi Crystal structure of parallel quadruplexes from human telomeric DNA
    Gary N Parkinson
    The Cancer Research UK Biomolecular Structure Unit, Chester Beatty Laboratories, The Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK
    Nature 417:876-80. 2002
    ..This DNA structure suggests a straightforward path for telomere folding and unfolding, as well as ways in which it can recognize telomere-associated proteins...
  52. pmc Statistical potential for assessment and prediction of protein structures
    Min Yi Shen
    Department of Biopharmaceutical Sciences, Department of Pharmaceutical Chemistry, University of California at San Francisco, San Francisco, California 94158, USA
    Protein Sci 15:2507-24. 2006
    ....
  53. ncbi Structure of Bcl-xL-Bak peptide complex: recognition between regulators of apoptosis
    M Sattler
    Pharmaceutical Discovery Division, Abbott Laboratories, Abbott Park, IL 60064, USA
    Science 275:983-6. 1997
    ..Mutations in full-length Bak that disrupt either type of interaction inhibit the ability of Bak to heterodimerize with Bcl-xL...
  54. ncbi Recognition of cognate transfer RNA by the 30S ribosomal subunit
    J M Ogle
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Science 292:897-902. 2001
    ..The third, or "wobble," position of the codon is free to accommodate certain noncanonical base pairs. By partially inducing these structural changes, paromomycin facilitates binding of near-cognate tRNAs...
  55. ncbi Insights into DNA recombination from the structure of a RAD51-BRCA2 complex
    Luca Pellegrini
    University of Cambridge, Department of Biochemistry, Tennis Court Road, Cambridge CB2 1GA, UK
    Nature 420:287-93. 2002
    ..Cancer-associated mutations that affect the BRC repeat disrupt its predicted interaction with RAD51, yielding structural insight into mechanisms for cancer susceptibility...
  56. ncbi Structural basis of chaperone function and pilus biogenesis
    F G Sauer
    Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
    Science 285:1058-61. 1999
    ..The structure of the PapD-PapK complex also suggests that during pilus biogenesis, every subunit completes the immunoglobulin-like fold of its neighboring subunit via a mechanism termed donor strand exchange...
  57. ncbi Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution
    R B Sutton
    The Howard Hughes Medical Institute, Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Nature 395:347-53. 1998
    ..These characteristics may be important for membrane fusion and for the binding of regulatory factors affecting neurotransmission...
  58. ncbi The retinal conformation and its environment in rhodopsin in light of a new 2.2 A crystal structure
    Tetsuji Okada
    Biological Information Research Center, National Institute of Advanced Industrial Science and Technology, 2 41 6 Aomi, Koto ku, Tokyo 135 0064, Japan
    J Mol Biol 342:571-83. 2004
    ..Other differences between the experimental and theoretical structures in the chromophore binding site are discussed with respect to the unique spectral properties and excited state reactivity of the chromophore...
  59. ncbi Structure of the C-terminal region of p21(WAF1/CIP1) complexed with human PCNA
    J M Gulbis
    Laboratory of Molecular Biophysics, The Rockefeller University, New York, New York 10021, USA
    Cell 87:297-306. 1996
    ..The ability of p21 to inhibit the action of PCNA is therefore likely to be due to its masking of elements on PCNA that are required for the binding of other components of the polymerase assembly...
  60. ncbi Chemistry of ion coordination and hydration revealed by a K+ channel-Fab complex at 2.0 A resolution
    Y Zhou
    Department of Molecular Biophysics and Biochemistry, Yale University, 260 Whitney Avenue, New Haven, Connecticut 06520, USA
    Nature 414:43-8. 2001
    ..This structural change is crucial to the operation of the selectivity filter in the cellular context, where the K+ ion concentration near the selectivity filter varies in response to channel gating...
  61. ncbi PHENIX: building new software for automated crystallographic structure determination
    Paul D Adams
    Lawrence Berkeley National Laboratory, One Cyclotron Road, Mailstop 4 230, Berkeley, CA 94720, USA
    Acta Crystallogr D Biol Crystallogr 58:1948-54. 2002
    ..This new software will provide the necessary algorithms to proceed from reduced intensity data to a refined molecular model and to facilitate structure solution for both the novice and expert crystallographer...
  62. ncbi Crystal structure of human serum albumin at 2.5 A resolution
    S Sugio
    Osaka Laboratories, Yoshitomi Pharmaceutical Industries Ltd, 2 25 1, Shodai Ohtani, Hirakata, Osaka 573 1153, Japan
    Protein Eng 12:439-46. 1999
    ..Three tentative binding sites for long-chain fatty acids, each with different surroundings, are located at the surface of each domain...
  63. ncbi Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast
    E Mossessova
    Biochemistry Department, Weill Medical College of Cornell University, New York, New York 10021, USA
    Mol Cell 5:865-76. 2000
    ..Genetic analysis guided by the structure further reveals a regulatory element N-terminal to the proteolytic domain that is required for cell growth in yeast...
  64. ncbi Version 1.2 of the Crystallography and NMR system
    Axel T Brunger
    The Howard Hughes Medical Institute and Department of Molecular and Cellular Physiology, Stanford University, California 94305, USA
    Nat Protoc 2:2728-33. 2007
    ..Other advances include the ability to apply thermal factor sharpening to electron density maps. Consistent with the modular design of CNS, these additions and changes were implemented in the high-level computing language of CNS...
  65. ncbi Stable, soluble T-cell receptor molecules for crystallization and therapeutics
    Jonathan M Boulter
    Avidex Ltd 57 Milton Park, Abingdon, Oxon OX14 4RX, UK
    Protein Eng 16:707-11. 2003
    ..Here we report the engineering of soluble human TCRs suitable for crystallization studies and potentially for in vivo therapeutic use...
  66. pmc Structural basis of activity and allosteric control of diguanylate cyclase
    Carmen Chan
    Divisions of Structural Biology and Molecular Microbiology, Biozentrum, University of Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland
    Proc Natl Acad Sci U S A 101:17084-9. 2004
    ..This allosteric site explains the observed noncompetitive product inhibition. We propose that product inhibition is due to domain immobilization and sets an upper limit for the concentration of this second messenger in the cell...
  67. ncbi Molecular model for a complete clathrin lattice from electron cryomicroscopy
    Alexander Fotin
    Biophysics Graduate Program, Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 432:573-9. 2004
    ..These invariant local interactions appear to stabilize the lattice, allowing assembly and uncoating to be controlled by events at a few specific sites...
  68. ncbi Crystal structures of histone demethylase JMJD2A reveal basis for substrate specificity
    Stanley S Ng
    Structural Genomics Consortium, Botnar Research Center, University of Oxford, Oxford OX3 7LD, UK
    Nature 448:87-91. 2007
    ....
  69. ncbi Crystal structure of a p53 tumor suppressor-DNA complex: understanding tumorigenic mutations
    Y Cho
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021
    Science 265:346-55. 1994
    ..The structure supports the hypothesis that DNA binding is critical for the biological activity of p53, and provides a framework for understanding how mutations inactivate it...
  70. ncbi Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody
    P D Kwong
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA
    Nature 393:648-59. 1998
    ..Our results provide a framework for understanding the complex biology of HIV entry into cells and should guide efforts to intervene...
  71. pmc Insights into translational termination from the structure of RF2 bound to the ribosome
    Albert Weixlbaumer
    Medical Research Council MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Science 322:953-6. 2008
    ..The structure provides insight into how RF2 specifically recognizes the stop codon; it also suggests a model for the role of a universally conserved GGQ motif in the catalysis of peptide release...
  72. ncbi Crystal structures of the kinase domain of c-Abl in complex with the small molecule inhibitors PD173955 and imatinib (STI-571)
    Bhushan Nagar
    Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, California 94720, USA
    Cancer Res 62:4236-43. 2002
    ..The structures suggest that PD173955 achieves its greater potency over STI-571 by being able to target multiple forms of Abl (active or inactive), whereas STI-571 requires a specific inactive conformation of Abl...
  73. ncbi Cellular motility driven by assembly and disassembly of actin filaments
    Thomas D Pollard
    Department of Cellular, Molecular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Cell 112:453-65. 2003
    ..Profilin catalyzes the exchange of ADP for ATP, refilling the pool of ATP-actin monomers bound to profilin, ready for elongation...
  74. ncbi The structure of crystalline profilin-beta-actin
    C E Schutt
    Department of Chemistry, Henry H Hoyt Laboratory, Princeton University, New Jersey 08544
    Nature 365:810-6. 1993
    ..Profilin forms two extensive contacts with the actin ribbon, one of which appears to correspond to the solution contact in vitro...
  75. ncbi Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters
    Atsuko Yamashita
    Department of Biochemistry and Molecular Biophysics and
    Nature 437:215-23. 2005
    ..The structure reveals the architecture of this important class of transporter, illuminates the determinants of substrate binding and ion selectivity, and defines the external and internal gates...
  76. ncbi Crystal structures of a multidrug transporter reveal a functionally rotating mechanism
    Satoshi Murakami
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567 0047, Japan
    Nature 443:173-9. 2006
    ..The voluminous binding pocket is aromatic and allows multi-site binding. The structures indicate that drugs are exported by a three-step functionally rotating mechanism in which substrates undergo ordered binding change...
  77. ncbi PRODRG: a tool for high-throughput crystallography of protein-ligand complexes
    Alexander W Schuttelkopf
    Division of Biological Chemistry and Molecular Microbiology, Wellcome Trust Biocentre, School of Life Sciences, University of Dundee, Dow Street, DD1 5EH, Scotland
    Acta Crystallogr D Biol Crystallogr 60:1355-63. 2004
    ..However, tests with distorted starting coordinates show that PRODRG topologies perform better, both in terms of ligand geometry and of crystallographic R factors...
  78. ncbi A structural basis for the selection of dominant alphabeta T cell receptors in antiviral immunity
    Lars Kjer-Nielsen
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia
    Immunity 18:53-64. 2003
    ..These findings indicate that TCR immunodominance is associated with structural properties conferring receptor specificity and suggest a novel structural link between TCR ligation and intracellular signaling...
  79. ncbi Crystal structure of human serum albumin complexed with fatty acid reveals an asymmetric distribution of binding sites
    S Curry
    Blackett Laboratory, Imperial College, London, UK
    Nat Struct Biol 5:827-35. 1998
    ..These pockets are distributed asymmetrically throughout the HSA molecule, despite its symmetrical repeating domain structure...
  80. ncbi The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit
    D E Brodersen
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Cell 103:1143-54. 2000
    ..For each of these antibiotics, interactions with the 30S subunit suggest a mechanism for its effects on ribosome function...
  81. ncbi Crystal structure and functional analysis of Ras binding to its effector phosphoinositide 3-kinase gamma
    M E Pacold
    MRC Laboratory of Molecular Biology Hills Road CB2 2QH, Cambridge, United Kingdom
    Cell 103:931-43. 2000
    ..These unique Ras/PI3Kgamma interactions are likely to be shared by PI3Kalpha. The complex with Ras shows a change in the PI3K conformation that may represent an allosteric component of Ras activation...
  82. pmc Crystal structure of the extracellular segment of integrin alpha Vbeta3
    J P Xiong
    Renal Unit, Leukocyte Biology and Inflammation Program, Structural Biology Program, Massachusetts General Hospital and Harvard Medical School, 149 13th Street, Charlestown, MA 02129, USA
    Science 294:339-45. 2001
    ..MIDAS lies adjacent to a calcium-binding site with a potential regulatory function...
  83. ncbi Crystal structure of a Smad MH1 domain bound to DNA: insights on DNA binding in TGF-beta signaling
    Y Shi
    Department of Molecular Biology, Princeton University, Lewis Thomas Laboratory, New Jersey 08544, USA
    Cell 94:585-94. 1998
    ..This structure establishes a framework for understanding how Smad proteins may act in concert with other transcription factors in the regulation of TGF-beta-responsive genes...
  84. ncbi Structures of complement component C3 provide insights into the function and evolution of immunity
    Bert J C Janssen
    Crystal and Structural Chemistry, Bijvoet Center for Biomolecular Research, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Nature 437:505-11. 2005
    ....
  85. pmc Structure of the cross-beta spine of amyloid-like fibrils
    Rebecca Nelson
    Howard Hughes Medical Institute, UCLA DOE Institute for Genomics and Proteomics, Box 951570, UCLA, Los Angeles, California 90095 1570, USA
    Nature 435:773-8. 2005
    ..The structure illuminates the stability of amyloid fibrils, their self-seeding characteristic and their tendency to form polymorphic structures...
  86. ncbi Blu-Ice and the Distributed Control System: software for data acquisition and instrument control at macromolecular crystallography beamlines
    Timothy M McPhillips
    Stanford Synchrotron Radiation Laboratory, 2575 Sand Hill Road, MS 69, Menlo Park, CA 94025, USA
    J Synchrotron Radiat 9:401-6. 2002
    ..Blu-Ice and DCS are currently installed on four Stanford Synchrotron Radiation Laboratory crystallographic beamlines and are being implemented at sister light sources...
  87. ncbi Structure of a repair enzyme interrogating undamaged DNA elucidates recognition of damaged DNA
    Anirban Banerjee
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
    Nature 434:612-8. 2005
    ..The structure reveals a remarkably effective gate-keeping strategy for lesion discrimination and suggests a mechanism for oxoG insertion into the hOGG1 active site...
  88. ncbi Generation, representation and flow of phase information in structure determination: recent developments in and around SHARP 2.0
    G Bricogne
    Global Phasing Ltd, Sheraton House, Castle Park, Cambridge CB3 0AX, England
    Acta Crystallogr D Biol Crystallogr 59:2023-30. 2003
    ..It is concluded that there is still considerable scope for further improvements in the statistical analysis of phase information, which touch upon numerous fundamental issues related to data processing and experimental design...
  89. ncbi Multiparametric scaling of diffraction intensities
    Zbyszek Otwinowski
    Department of Biochemistry, University of Texas, Southwestern Medical Center at Dallas, TX 75390 9038, USA
    Acta Crystallogr A 59:228-34. 2003
    ..This scaling model can be extended to include additional corrections for various instrumental and data-collection problems...
  90. ncbi Prolyl oligopeptidase: an unusual beta-propeller domain regulates proteolysis
    V Fulop
    Department of Biochemistry, Oxford Centre for Molecular Sciences, University of Oxford, United Kingdom
    Cell 94:161-70. 1998
    ..In this way, the propeller protects larger peptides and proteins from proteolysis in the cytosol. The structure is also obtained with a transition state inhibitor, which may facilitate drug design to treat memory disorders...
  91. ncbi Structural comparisons of calponin homology domains: implications for actin binding
    S Banuelos
    European Molecular Biology Laboratory Postfach 10 2209 D 69012 Heidelberg, Germany
    Structure 6:1419-31. 1998
    ..The three-dimensional structures of three CH domains are known, but they have not yet clarified the molecular details of the interaction between actin filaments and proteins harbouring CH domains...
  92. ncbi Critical residues for structure and catalysis in short-chain dehydrogenases/reductases
    Charlotta Filling
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    J Biol Chem 277:25677-84. 2002
    ..This extends the previously recognized catalytic triad of Ser-Tyr-Lys residues to form a tetrad of Asn-Ser-Tyr-Lys in the majority of characterized short-chain dehydrogenases/reductase enzymes...
  93. ncbi Automated protein model building combined with iterative structure refinement
    A Perrakis
    European Molecular Biology Laboratory EMBL, Grenoble Outstation, France
    Nat Struct Biol 6:458-63. 1999
    ..Automatic pattern recognition (model building) combined with refinement, allows a structural model to be obtained reliably within a few CPU hours. We demonstrate the power of the method with examples of a few recently solved structures...
  94. ncbi Auto-rickshaw: an automated crystal structure determination platform as an efficient tool for the validation of an X-ray diffraction experiment
    Santosh Panjikar
    EMBL Hamburg Outstation, c o DESY, Notkestrasse 85, D 22603 Hamburg, Germany
    Acta Crystallogr D Biol Crystallogr 61:449-57. 2005
    ..The processes have been optimized for speed so that the pipeline can be used effectively for validating the X-ray experiment at a synchrotron beamline...
  95. ncbi DNA lesion recognition by the bacterial repair enzyme MutM
    J Christopher Fromme
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    J Biol Chem 278:51543-8. 2003
    ..Furthermore, the structure of MutM in complex with DNA containing an alternative substrate, dihydrouracil, demonstrates how MutM is able to recognize lesions other than oxoG...
  96. ncbi Structure of a bacterial multidrug ABC transporter
    Roger J P Dawson
    Institute of Molecular Biology and Biophysics, ETH Zurich, 8093 Zurich, Switzerland
    Nature 443:180-5. 2006
    ....
  97. ncbi Structural studies on 2-oxoglutarate oxygenases and related double-stranded beta-helix fold proteins
    Ian J Clifton
    The Oxford Centre for Molecular Sciences and the Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Mansfield Road, Oxford, Oxon OX1 3TA, UK
    J Inorg Biochem 100:644-69. 2006
    ..Proposals regarding the suitability of the active sites and folds of the 2-oxoglutarate oxygenases to catalyze reactions involving reactive oxidizing species are described...
  98. ncbi Energetic optimization of ion conduction rate by the K+ selectivity filter
    J H Morais-Cabral
    Department of Molecular Biophysics and Biochemistry, Yale University, 260 Whitney Avenue, New Haven, Connecticut 06520, USA
    Nature 414:37-42. 2001
    ..The energetic balance between these configurations is a clear example of evolutionary optimization of protein function...
  99. ncbi The structure of DNA in the nucleosome core
    Timothy J Richmond
    ETH Zurich, Institut fur Molekularbiologie und Biophysik, ETH Honggerberg, CH 8093 Zurich
    Nature 423:145-50. 2003
    ....
  100. ncbi Structures of the bacterial ribosome at 3.5 A resolution
    Barbara S Schuwirth
    Department of Chemistry, University of California, Berkeley, CA 94720, USA
    Science 310:827-34. 2005
    ....
  101. ncbi Structures of HIV-1 gp120 envelope glycoproteins from laboratory-adapted and primary isolates
    P D Kwong
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA
    Structure 8:1329-39. 2000
    ..This revealed atomic details of gp120-receptor interactions and suggested multiple mechanisms of immune evasion...

Research Grants72

  1. STRUCTURE-BASED TUBERCULOSIS DRUG DESIGN TARGETED AT ACYL-COA CARBOXYLASE
    Shiou Chuan Tsai; Fiscal Year: 2010
    ..PERFORMANCE SITE(S) (organization, city, state) University of California, Irvine, CA 92697, USA REVISED ABSTRACT SECTION ..
  2. Structure and function of urea transporters
    Ming Zhou; Fiscal Year: 2013
    ..Compounds that selectively block urea transporter will have diuretic effect but will not interfere with the salt balance. ..
  3. BIOSYNTHESIS OF TRACHEAL MUCOUS GLYCOPROTEINS
    Pi Wan Cheng; Fiscal Year: 2010
    ..Current studies could facilitate the development of therapy for mucus hypersecretory diseases through identification of small carbohydrate inhibitors and mucus cell-specific promoter. ..
  4. Structural and molecular basis of drug-induced IKACh reduction
    SAMI FOUAD NOUJAIM; Fiscal Year: 2012
    ..abstract_text> ..
  5. Structural and molecular basis of drug-induced IKACh reduction
    SAMI FOUAD NOUJAIM; Fiscal Year: 2013
    ..abstract_text> ..
  6. Interdisciplinary Research Training in Reproduction for Indian Fellows
    John C Herr; Fiscal Year: 2010
    ..abstract_text> ..
  7. Systemic AA Amyloidosis Inhibitors
    ALAN D SNOW; Fiscal Year: 2010
    ..In this project we intend to develop a new drug (and back-up) for effective treatment of ~100,000 cases of systemic amyloidosis in the USA, for which today there is no real treatment whatsoever. ..
  8. MECHANISM-BASED DRUG SELECTION AND DESIGN: NUCLEOTIDE SA
    Buddy Ullman; Fiscal Year: 2003
    ..Concurrent experiments will determine how NTPase functions in AK HGXPRT-, and AT parasites, identify the protein(s) which regulate NTPase enzymatic activity, and evaluate the impact of abrogating NTPase expression on parasite viability. ..
  9. CHEMISTRY AND BIOLOGY OF PLATINUM ANTICANCER DRUGS
    Stephen J Lippard; Fiscal Year: 2013
    ..The knowledge acquired will be used to design, synthesize, and evaluate new anticancer drug candidates for targeting and destroying tumors. ..
  10. Regulation of NFkappaB activity by the Estrogen Receptor
    Kendall W Nettles; Fiscal Year: 2012
    ..We propose to use these synthetic compounds to understand the molecular details of this important anti-inflammatory pathway. ..
  11. Ferritin: Protein/mRNA/DNA in Fe/O Regulation/Metabolism
    ELIZABETH C contact THEIL; Fiscal Year: 2010
    ....
  12. FUNCTIONAL PROPERTIES OF HEMOGLOBINS AND MYOGLOBINS
    John S Olson; Fiscal Year: 2010
    ....
  13. MECHANISMS OF FGF RECEPTOR REGULATION AND SIGNALING
    Moosa Mohammadi; Fiscal Year: 2013
    ..Since substrate recruitment and phosphorylation by RTKs is a general event in RTK signaling, the mechanistic insights gained under Aim IV will also be directly applicable to the entire RTK superfamily. ..
  14. KINETICS OF DRUG MACROMOLECULE COMPLEX FORMATION
    PALMER WILLIAM TAYLOR; Fiscal Year: 2013
    ..Our structural and functional studies, using X ray crystallography and solution-based spectroscopic and spectrometric techniques, offer new avenues into understanding the ..
  15. Nitric Oxide Signaling with Heme Proteins
    William R Montfort; Fiscal Year: 2012
    ..Our results are expected to uncover new approaches for drug discovery targeted to cardiovascular disease, cancer metastasis and related failures in human health. ..
  16. Identifying Key Structural Elements in the Activation of the Arp2/3 Complex
    Patricia A Khuu; Fiscal Year: 2013
    ..Arp2/3 is also involved in metastasis as it is required in the formation of cellular structures such as lamellipodia of migrating cells and invadopodia of cancers cells [5, 6]. ..
  17. Biosynthetic Protein Models of Heme-copper Oxidases and Nitric Oxide Reductases
    Yi Lu; Fiscal Year: 2013
    ..Therefore the work will make important contributions to healthcare, as it will provide a molecular basis for understanding two enzymes important to human health. ..
  18. Thrombospondin 4 A1
    JOHN W LAWLER; Fiscal Year: 2010
    ..The data from these studies will provide key insights into the function of the signature domain of COMP in terms of its interactions with various proteins, PGs and cells. ..
  19. X-ray Studies of Sugar-Modifying Enzymes
    Hazel Holden; Fiscal Year: 2013
    ....
  20. Metabolic Pathways and Defects in Fructose Metabolism
    DEAN TOLAN; Fiscal Year: 2009
    ..Lastly, blood samples from African-American and Hispanic-American HFI subjects will be used for identification of gene defects by direct DNA sequencing, thus offering a reliable non-invasive diagnostic method to these Americans. ..
  21. Structure and function of chloride channels and transporters
    Alessio Accardi; Fiscal Year: 2013
    ..unreadable][unreadable] ..
  22. Carbohydrate Regulation of Hepatic Gene Expression
    Kosaku Uyeda; Fiscal Year: 2010
    ..This knowledge will provide information that may be useful in developing strategies for the prevention and treatment of obesity. ..
  23. The role fo the TPP1 peotein in telomerase function and cancer cell survival
    Jayakrishnan Nandakumar; Fiscal Year: 2013
    ..I believe that the knowledge and experience I have gained thus far will greatly assist in the successful completion of the aims of the K99/R00 proposal in a timely fashion. ..
  24. Ligand Interaction of Glucagon Receptor by Structural and Functional Analyses
    FAI YIU SIU; Fiscal Year: 2012
    ....
  25. Structure and Mechanism of SAM-responsive Riboswitches
    Robert T Batey; Fiscal Year: 2013
    ..A deeper knowledge of how RNA specifically interacts with small molecules will help pave the way for a new generation of therapeutics that target non-protein coding RNAs that are pervasive in both bacteria and eukaryotes. ..
  26. Nonheme Diiron Centers and the Biological Oxidation of Hydrocarbons
    Stephen J Lippard; Fiscal Year: 2013
    ..The ability of these hydrocarbon-consuming "superbugs" to degrade chlorinated hydrocarbons has led to their application in the bioremediation of industrial wastewater, decontamination of seawater, and removal of soil pollutants. ..
  27. Structure and Function of Enzymes in Fatty Acid Oxidation
    JUNG JA P KIM; Fiscal Year: 2013
    ....
  28. Challenges in beta-Lactamase Mediated Resistance
    Robert A Bonomo; Fiscal Year: 2013
    ..We chose these [unreadable]-lactamases as they are among the most important class A and class C enzymes in Gram-negative bacteria. ..
  29. Problems in Membrane Protein Crystallography: Hetero-Oligomeric Cytochrome b6f
    William A Cramer; Fiscal Year: 2012
    ..Via the membrane, the set of energy-transducing proteins determines the level of energy and its regulation in the human cell. ..
  30. Regulation of Carbohydrate Metabolism and Lipogenesis
    Kosaku Uyeda; Fiscal Year: 2013
    ..X-ray crystallography will be used to investigate the mechanisms of metabolite regulation of ChREBP binding to 14-3-3 and importin [unreadable] at an atomic level. ..
  31. Separation-of-function Mutants to Study the Biological Significance of Telomerase
    Jayakrishnan Nandakumar; Fiscal Year: 2012
    ..From this study, it will be possible to understand how TPP1 stimulates telomerase to full potency in cancer cells, and the knowledge gained may pave the path to the design of a new generation of anti-cancer drugs. ..
  32. Structure-Based Design of Novel HNF4a Antagonists to Treat Metabolic Syndrome
    Arnold T Hagler; Fiscal Year: 2010
    ..To develop a drug to address this complex disease, we will take a novel approach, designing compounds that target the nuclear receptor HNF4a, which is involved in regulating all components of this syndrome. ..
  33. The influenza A virus nucleoprotein: structure and function
    Yizhi Jane Tao; Fiscal Year: 2012
    ..The aim of this grant is to elucidate the molecular mechanism and biological significance of several key activities of the influenza A virus nucleoprotein and to discover how such activities can be exploited for antiviral development. ..
  34. MOLECULAR BASIS OF ALPHAVIRUS ASSEMBLY
    Richard J Kuhn; Fiscal Year: 2013
    ..This insight will enable us, and others, to pursue novel strategies for virus and disease intervention. ..
  35. Mechanisms and regulation of serine resolvases
    Phoebe A Rice; Fiscal Year: 2012
    ..This work will lead to a better understanding of genetic transactions in bacteria, enhancing the predictive power of sequence databases and leading toward ways to control resistance genes in bacteria. ..
  36. Pathogen-Specific Regulation of Protein Assembly
    ALLEN BERNARD REITZ; Fiscal Year: 2010
    ..We first seek to apply our strategy to the discovery of agents to treat Yersinia enterocolitica, an NIAID Category B priority pathogen. ..
  37. Recombinant Antibodies from the Sea Lamprey
    ZEEV contact PANCER; Fiscal Year: 2010
    ..We propose to develop high affinity recombinant antibodies from the sea lamprey as a completely new class of recognition molecules for diagnostic, analytic, and biosensor applications in biotechnology. ..
  38. Molecular Basis of Bacterial Quorum Sensing Gene Regulation
    MAIR E CHURCHILL; Fiscal Year: 2010
    ..abstract_text> ..
  39. Investigation of the structure and function of urea amidolyase
    MARTIN RAOUL ST. MAURICE; Fiscal Year: 2012
    ..Achieving the goals of this project will result in a greatly enhanced description of UAL structure and function, providing necessary insights for the eventual design of UAL-specific inhibitors with therapeutic potential. ..
  40. Structural Immunology of CD1 and CD1-TCR Complexes
    Dirk M Zajonc; Fiscal Year: 2013
    ..Understanding the functional properties of these cell types at a molecular level is crucial for the future development of novel chemotherapeutic agents or adjuvants to combat both diseases. ..
  41. Structural Basis for Synergistic Gene Expression by Runx1 and Ets1 Proteins
    Tahir H Tahirov; Fiscal Year: 2012
    ..Success with the proposed structural studies ultimately will lead to the discovery of novel therapies for the prevention and treatment of leukemia and other cancers. ..
  42. Plasmid-Mediated Quinolone Resistance
    George A Jacoby; Fiscal Year: 2013
    ..Under Specific Aim 3, we propose to explore Qnr/gyrase interaction as revealed by isothermal titration calorimetry or surface plasmon resonance and by x- ray crystallography. ..
  43. Mechanisms of Viral DNA Packaging
    CARLOS JOSE BUSTAMANTE; Fiscal Year: 2013
    ..Finally, we wish to investigate how the increasing internal DNA pressure regulates the dynamics of the motor during packaging. ..
  44. Facilitating large scale biomass generation: acquisition of an automated 100 L fe
    Roger D Kornberg; Fiscal Year: 2010
    ..Growth of cell cultures on a large scale in the proposed system will permit the isolation of proteins involved in these fundamental processes. The isolated proteins will be subjected to biochemical and structural analyses. ..
  45. Characterization of CHD family chromodomains Implicated in Epigenetic Signaling
    Kimberly R Wiggins; Fiscal Year: 2010
    ..To better understand epigenetic regulation, we will use structural biology and biochemistry methods to delineate the signalling mechanisms in a family of human proteins. ..
  46. Structural Genomics of Orphan Nuclear Receptors
    H Eric Xu; Fiscal Year: 2013
    ....
  47. 3D Structure and Function of CRAC Channels
    STEPHEN BARSTOW LONG; Fiscal Year: 2013
    ....
  48. STRUCTURAL STUDIES ON PROKARYOTIC POTASSIUM CHANNELS
    Adrian Gross; Fiscal Year: 2013
    ..Determining the precise structure and mechanism of action of these channels will allow for the development of safer and more effective drugs. ..
  49. Structural and Biochemical Basis of the Vitamin K cycle
    Weikai Li; Fiscal Year: 2013
    ..Future research in my group will use a combination of X-ray crystallography, protein biochemistry, and cell biology. My long-term goal is to understand the entire vitamin K pathway that sustains blood coagulation. ..
  50. Control of cap-independent translation by a viral 3'UTR
    WYATT ALLEN MILLER; Fiscal Year: 2013
    ..Over-active eIF4E causes tumors and restriction of its function inhibits many types of cancers. The tightly binding PTE RNA may provide structural knowledge for design of eIF4E-inhibiting cancer therapeutics. ..
  51. Uncovering the Regulatory Role of gH/gL in HSV Fusion
    Roselyn J Eisenberg; Fiscal Year: 2013
    ..My research may suggest new therapeutic targets that will result in new treatments to stop the virus from gaining entry into its favorite target cells. ..
  52. Structure and dynamics of the Tetrahymena telomerase ribonucleoprotein
    Michael D Stone; Fiscal Year: 2013
    ..To this end, this proposal aims to illuminate the structural properties of telomerase that underlie its unique cellular activity. ..
  53. Chemical Glycobiology on Anthracyclines
    Peng Wang; Fiscal Year: 2009
    ..The success of such a platform will accelerate new drug discovery in the field of anticancer drug involving DNA-enzyme-drug complex. This research program should produce new generations of preclinical anthracycline drug candidates. ..
  54. Characterization of purified myocilin: glaucoma as a protein misfolding disease
    Raquel L Lieberman; Fiscal Year: 2013
    ....
  55. Molecular mechanisms of potassium channel permeation and gating
    Crina M Nimigean; Fiscal Year: 2013
    ..Specifically, we seek to understand how potassium channels allow certain ions to cross their pores with exquisite discrimination, as well as investigating the mechanism of how these channels open and close, a process called gating. ..
  56. Small Molecule Inhibitors for the Oncogenic Protein Tyrosine Phosphatase SHP2
    Zhong Yin Zhang; Fiscal Year: 2013
    ..In addition, potent and selective SHP2 inhibitors will also serve as powerful research tools to delineate the function of SHP2 in normal physiology and to elucidate the events underlying SHP2-evoked transformation. ..
  57. Lectin Resistant Tumor Cells And Functional Glycomics
    PAMELA M STANLEY; Fiscal Year: 2013
    ..The molecular basis of the requirement for MGAT1 will be investigated by candidate and unbiased approaches. ..
  58. Structure of sclerostin protein complexes
    Rajiv Kumar; Fiscal Year: 2012
    ..This effort will greatly help women and men with osteoporosis and osteoporosis-related fractures. ..
  59. Mechanism of Slow Onset Enzyme Inhibition and Drug Target Residence Time
    Peter J Tonge; Fiscal Year: 2013
    ..Demonstrating the importance of tR will lead to a paradigm shift in lead compound optimization. ..
  60. Design and mechanistic studies of mimics of antimicrobial peptides
    William F Degrado; Fiscal Year: 2013
    ..We will also discover small molecule inhibitors of this and other HKs, and use them to probe the roles of HKs in colonization and virulence in animal models. ..
  61. Structural Biochemistry of DNA Base Repair
    John A Tainer; Fiscal Year: 2012
    ..abstract_text> ..
  62. Imaging Nuclear Receptor LRH-1 in Functional Transcriptional Assemblies
    Robert J Fletterick; Fiscal Year: 2010
    ....
  63. Structure and mechanism of the dynein motor
    Ronald D Vale; Fiscal Year: 2013
    ....
  64. Bright and Fast Sensor for Time Resolved X-Ray Diffraction Studies
    VIVEK V NAGARKAR; Fiscal Year: 2010
    ....
  65. ION PAIR ALDOL ADDITION REACTIONS
    Andrew Streitwieser; Fiscal Year: 1999
    ..It is suggested that subsequent extension to other salts of alkali and alkaline earth metals, early and late transition metals and lanthanides is also proposed since many of these salts have found use in some stereospecific syntheses. ..
  66. Apparatus for encapsulating integral membrane proteins for structural studies by
    RONALD WILLIAM PETERSON; Fiscal Year: 2010
    ..If successful, this technology could serve as a powerful platform for the rational design of pharmaceuticals for the treatment of an array of human diseases. ..
  67. STRUCTURAL STUDIES OF DNA REPLICATION AND REPAIR
    Gabriel Waksman; Fiscal Year: 2003
    ..Such structures will provide information on the mechanism of active unwinding by DNA-helicases and of passive unwinding by helix-destabilizing SSB proteins. ..
  68. Novel Simulated Moving Bed Chromatography Device to Purify Recombinant Proteins
    ROBERT MIERENDORF; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable] [unreadable]..
  69. X RAY STRUCTURAL STUDIES OF LACTOFERRIN
    Edward Baker; Fiscal Year: 2000
    ..6. To complete a study of the effects of glycosylation on the structure and function of transferrin. ..
  70. Structural Contexts and Functional Consequences of Protein S-nitrosylation
    Eric R Schreiter; Fiscal Year: 2010
    ..This information will aid the design of therapeautic treatments to disorders involving aberrant protein S-nitrosylation. ..
  71. Anachem Lipidic Cubic Phase Crystallization Robot
    Robert M Stroud; Fiscal Year: 2010
    ..This proposal seeks to advance structural determination of membrane proteins in general, and targets human or pathogenic membrane proteins of importance to human health. ..
  72. Bright and Fast Sensor for Time Resolved X-Ray Diffraction Studies
    Vivek Nagarkar; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..