crystallization

Summary

Summary: The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

Top Publications

  1. ncbi Roll-to-roll production of 30-inch graphene films for transparent electrodes
    Sukang Bae
    SKKU Advanced Institute of Nanotechnology and Center for Human Interface Nano Technology, Sungkyunkwan University, Suwon 440 746, Korea
    Nat Nanotechnol 5:574-8. 2010
  2. ncbi Design and self-assembly of two-dimensional DNA crystals
    E Winfree
    Computation and Neural Systems, California Institute of Technology, Pasadena 91125, USA
    Nature 394:539-44. 1998
  3. pmc High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor
    Vadim Cherezov
    Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    Science 318:1258-65. 2007
  4. ncbi Self-assembly at all scales
    George M Whitesides
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Science 295:2418-21. 2002
  5. ncbi Automated structure solution with the PHENIX suite
    Peter H Zwart
    Lawrence Berkeley National Laboratory, Berkeley, CA, USA
    Methods Mol Biol 426:419-35. 2008
  6. pmc Structure of a beta1-adrenergic G-protein-coupled receptor
    Tony Warne
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Nature 454:486-91. 2008
  7. pmc Fusion partner toolchest for the stabilization and crystallization of G protein-coupled receptors
    Eugene Chun
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Structure 20:967-76. 2012
  8. ncbi Self-assembly and mineralization of peptide-amphiphile nanofibers
    J D Hartgerink
    Department of Materials Science and Engineering, Medical School, Northwestern University, 2225 North Campus Drive, Evanston, IL 60208, USA
    Science 294:1684-8. 2001
  9. pmc Chemical screening methods to identify ligands that promote protein stability, protein crystallization, and structure determination
    Masoud Vedadi
    Structural Genomics Consortium, University of Toronto, 100 College Street, Toronto, ON, Canada M5G 1L5
    Proc Natl Acad Sci U S A 103:15835-40. 2006
  10. pmc Crystallizing membrane proteins using lipidic mesophases
    Martin Caffrey
    Membrane Structural and Functional Biology Group, University of Limerick, Limerick, Ireland
    Nat Protoc 4:706-31. 2009

Detail Information

Publications408 found, 100 shown here

  1. ncbi Roll-to-roll production of 30-inch graphene films for transparent electrodes
    Sukang Bae
    SKKU Advanced Institute of Nanotechnology and Center for Human Interface Nano Technology, Sungkyunkwan University, Suwon 440 746, Korea
    Nat Nanotechnol 5:574-8. 2010
    ..Graphene electrodes were incorporated into a fully functional touch-screen panel device capable of withstanding high strain...
  2. ncbi Design and self-assembly of two-dimensional DNA crystals
    E Winfree
    Computation and Neural Systems, California Institute of Technology, Pasadena 91125, USA
    Nature 394:539-44. 1998
    ..The patterned crystals have been visualized by atomic force microscopy...
  3. pmc High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor
    Vadim Cherezov
    Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    Science 318:1258-65. 2007
    ..Cholesterol, a necessary component for crystallization, mediates an intriguing parallel association of receptor molecules in the crystal lattice...
  4. ncbi Self-assembly at all scales
    George M Whitesides
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Science 295:2418-21. 2002
    ..The concept of self-assembly is used increasingly in many disciplines, with a different flavor and emphasis in each...
  5. ncbi Automated structure solution with the PHENIX suite
    Peter H Zwart
    Lawrence Berkeley National Laboratory, Berkeley, CA, USA
    Methods Mol Biol 426:419-35. 2008
    ..The algorithms are tightly linked and made easily accessible to users through the PHENIX Wizards and the PHENIX GUI...
  6. pmc Structure of a beta1-adrenergic G-protein-coupled receptor
    Tony Warne
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Nature 454:486-91. 2008
    ....
  7. pmc Fusion partner toolchest for the stabilization and crystallization of G protein-coupled receptors
    Eugene Chun
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Structure 20:967-76. 2012
    ....
  8. ncbi Self-assembly and mineralization of peptide-amphiphile nanofibers
    J D Hartgerink
    Department of Materials Science and Engineering, Medical School, Northwestern University, 2225 North Campus Drive, Evanston, IL 60208, USA
    Science 294:1684-8. 2001
    ..This alignment is the same as that observed between collagen fibrils and hydroxyapatite crystals in bone...
  9. pmc Chemical screening methods to identify ligands that promote protein stability, protein crystallization, and structure determination
    Masoud Vedadi
    Structural Genomics Consortium, University of Toronto, 100 College Street, Toronto, ON, Canada M5G 1L5
    Proc Natl Acad Sci U S A 103:15835-40. 2006
    ..The 3D structures of human therapeutic targets are enabling for drug discovery. However, their purification and crystallization remain rate determining...
  10. pmc Crystallizing membrane proteins using lipidic mesophases
    Martin Caffrey
    Membrane Structural and Functional Biology Group, University of Limerick, Limerick, Ireland
    Nat Protoc 4:706-31. 2009
    ..Methods for harvesting microcrystals are also described. The time required to prepare the protein-loaded mesophase and to set up a crystallization plate manually is about 1 h.
  11. ncbi Crystal structure of the human beta2 adrenergic G-protein-coupled receptor
    Søren G F Rasmussen
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, 279 Campus Drive, Stanford, Palo Alto, California 94305, USA
    Nature 450:383-7. 2007
    ..These differences may be responsible for the relatively high basal activity and structural instability of the beta2AR, and contribute to the challenges in obtaining diffraction-quality crystals of non-rhodopsin GPCRs...
  12. pmc Structure and function of an irreversible agonist-β(2) adrenoceptor complex
    Daniel M Rosenbaum
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, 279 Campus Drive, Stanford, California 94305, USA
    Nature 469:236-40. 2011
    ..However, the crystallization of agonist-bound GPCRs has been hampered by modest affinities and rapid off-rates of available agonists...
  13. ncbi Matrix Gla protein inhibits ectopic calcification by a direct interaction with hydroxyapatite crystals
    Jason O'Young
    School of Dentistry, University of Western Ontario, London, Canada
    J Am Chem Soc 133:18406-12. 2011
    ..The strong correlation between simulated adsorption and measured growth inhibition indicates that MD provides a powerful tool to predict the effects of proteins and peptides on crystal formation...
  14. pmc Nanocrystal technology, drug delivery and clinical applications
    Jens Uwe A H Junghanns
    Department of Pharmaceutical Technology, Biotechnology and Quality Management, Free University of Berlin, Berlin, Germany
    Int J Nanomedicine 3:295-309. 2008
    ..Examples of products in the development pipelines (clinical phases) are presented and the benefits for in vivo administration of drug nanocrystals are summarized in an overview...
  15. ncbi Stable, soluble T-cell receptor molecules for crystallization and therapeutics
    Jonathan M Boulter
    Avidex Ltd 57 Milton Park, Abingdon, Oxon OX14 4RX, UK
    Protein Eng 16:707-11. 2003
    ..Here we report the engineering of soluble human TCRs suitable for crystallization studies and potentially for in vivo therapeutic use.
  16. ncbi Photonic crystal surface waves for optical biosensors
    Valery N Konopsky
    Anal Chem 79:4729-35. 2007
    ..The detection limit is approximately 20 fg of the analyte on the probed spot of the surface...
  17. ncbi Crystal structure of the 30 S ribosomal subunit from Thermus thermophilus: purification, crystallization and structure determination
    W M Clemons
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    J Mol Biol 310:827-43. 2001
    We describe the crystallization and structure determination of the 30 S ribosomal subunit from Thermus thermophilus...
  18. pmc Maltose-neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteins
    Pil Seok Chae
    Department of Chemistry, University of Wisconsin Madison, Madison, Wisconsin, USA
    Nat Methods 7:1003-8. 2010
    ..as manifested in multiple membrane protein systems, leading to enhanced structural stability and successful crystallization. MNG amphiphiles are promising tools for membrane protein science because of the ease with which they may be ..
  19. ncbi Seeds to crystals
    Terese Bergfors
    Department of Cell and Molecular Biology, Biomedical Center, Uppsala University, Box 596, 751 24, Uppsala, Sweden
    J Struct Biol 142:66-76. 2003
    ..The implementation of seeding in high-throughput crystallization, robotics, and other emerging technologies is also discussed.
  20. ncbi The structure of the potassium channel: molecular basis of K+ conduction and selectivity
    D A Doyle
    Laboratory of Molecular Neurobiology and Biophysics and the Howard Hughes Medical Institute, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 280:69-77. 1998
    ..The architecture of the pore establishes the physical principles underlying selective K+ conduction...
  21. ncbi Increased osteoblast adhesion on nanophase metals: Ti, Ti6Al4V, and CoCrMo
    Thomas J Webster
    Department of Biomedical Engineering, Purdue University, 1296Patter Building, West Lafayette, IN 47905, USA
    Biomaterials 25:4731-9. 2004
    ....
  22. pmc The role of neutral lipid nanospheres in Plasmodium falciparum haem crystallization
    John M Pisciotta
    Malaria Research Institute, W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 N Wolfe Street, Baltimore, MD 21205, USA
    Biochem J 402:197-204. 2007
    ..Haem crystallization is the target of the widely used antimalarial quinoline drugs...
  23. ncbi Activation of liver X receptor sensitizes mice to gallbladder cholesterol crystallization
    Hirdesh Uppal
    Center for Pharmacogenetics, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Hepatology 47:1331-42. 2008
    ..of nuclear receptor liver X receptor (LXR) sensitized mice to lithogenic diet-induced gallbladder cholesterol crystallization, which was associated with dysregulation of several hepatic transporters that efflux cholesterol, ..
  24. ncbi Detergents for the stabilization and crystallization of membrane proteins
    Gilbert G Prive
    Division of Cancer Genomics and Proteomics, Ontario Cancer Institute, Department of Medical Biophysics, University of Toronto, 101 College Street, Toronto, Ontario, Canada
    Methods 41:388-97. 2007
    ..Empirical "stability screens" can be helpful in choosing which detergents, and which detergent concentrations, may be optimal for a given system...
  25. pmc Unlocking the eukaryotic membrane protein structural proteome
    John Kyongwon Lee
    Department of Biochemistry and Biophysics, University of California, San Francisco, S 412C Genentech Hall, 600 16th Street, San Francisco, CA 94158 2517, United States
    Curr Opin Struct Biol 20:464-70. 2010
    ..The first of these appeared in 2005. Our review addresses the special issues that pertain to the expression of eukaryotic and human membrane proteins, and recent advances in the tool kit for crystallization and structure determination.
  26. ncbi Replication by human DNA polymerase-iota occurs by Hoogsteen base-pairing
    Deepak T Nair
    Structural Biology Program, Department of Physiology and Biophysics, Mount Sinai School of Medicine, Box 1677, 1425 Madison Avenue, New York, New York 10029, USA
    Nature 430:377-80. 2004
    ....
  27. ncbi Structural characterization of the molecular platform for type III secretion system assembly
    Calvin K Yip
    Department of Biochemistry and Molecular Biology, University of British Columbia, 2146 Health Sciences Mall, Vancouver, British Columbia, Canada V6T 1Z3
    Nature 435:702-7. 2005
    ..We propose that the YscJ/PrgK protein family functions as an essential molecular platform for TTSS assembly...
  28. ncbi A new matrix protein family related to the nacreous layer formation of Pinctada fucata
    T Samata
    Laboratory of Cell Biology, Faculty of Environmental Health, Azabu University, 1 17 71 Fuchinobe, Sagamihara, Japan
    FEBS Lett 462:225-9. 1999
    ..The in vitro studies of the crystallization clarified that N16 induced aragonite crystals when fixed on the substrate but inhibited crystal formation ..
  29. pmc Insulin crystallization depends on zinc transporter ZnT8 expression, but is not required for normal glucose homeostasis in mice
    K Lemaire
    Gene Expression Unit, Department Molecular Cell Biology, Katholieke Universiteit Leuven, Herestraat 49, 3000 Leuven, Belgium
    Proc Natl Acad Sci U S A 106:14872-7. 2009
    ..Interaction between the ZnT8(-/-) genotype and diet to induce diabetes is a model for further studies of the mechanism of disease of human ZNT8 gene mutations...
  30. ncbi Crystal structure of the malaria vaccine candidate apical membrane antigen 1
    Juan Carlos Pizarro
    Unité d Immunologie Structurale, Centre National de la Recherche Scientifique, URA 2185, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris, France
    Science 308:408-11. 2005
    ..These results are likely to aid vaccine and drug design...
  31. ncbi Bicelle crystallization: a new method for crystallizing membrane proteins yields a monomeric bacteriorhodopsin structure
    Salem Faham
    Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095 1570, USA
    J Mol Biol 316:1-6. 2002
    ..0 A resolution. In all previous bR structures the protein is organized as a parallel trimer, but in the crystals grown from bicelles, the individual bR subunits are arranged in an antiparallel fashion...
  32. pmc Matthews coefficient probabilities: Improved estimates for unit cell contents of proteins, DNA, and protein-nucleic acid complex crystals
    Katherine A Kantardjieff
    Department of Chemistry and Biochemistry, California State University CSU Fullerton, Fullerton, California 92834 6866, USA
    Protein Sci 12:1865-71. 2003
    ....
  33. pmc In situ proteolysis for protein crystallization and structure determination
    Aiping Dong
    Structural Genomics Consortium, University of Toronto, 100 College Street, Toronto, Ontario M5G 1L5, Canada
    Nat Methods 4:1019-21. 2007
    ..suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at ..
  34. pmc From molecular to macroscopic via the rational design of a self-assembled 3D DNA crystal
    Jianping Zheng
    Department of Chemistry, New York University, New York 10003, USA
    Nature 461:74-7. 2009
    ..The data demonstrate clearly that it is possible to design and self-assemble a well-ordered macromolecular 3D crystalline lattice with precise control...
  35. ncbi Early stages of crystallization of calcium carbonate revealed in picoliter droplets
    Christopher J Stephens
    School of Physics and Astronomy, University of Leeds, Leeds LS2 9JT, United Kingdom
    J Am Chem Soc 133:5210-3. 2011
    ..CaCO(3) crystallization proceeds significantly more slowly in the droplets than in the bulk, allowing the mechanism of ..
  36. pmc N-terminal T4 lysozyme fusion facilitates crystallization of a G protein coupled receptor
    Yaozhong Zou
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California, United States of America
    PLoS ONE 7:e46039. 2012
    ..This approach adds to the protein engineering strategies that enable crystallographic studies of GPCRs alone or in complex with a signaling partner...
  37. ncbi Transfer-free electrical insulation of epitaxial graphene from its metal substrate
    Silvano Lizzit
    Sincrotrone Trieste, S S 14 Km 163 5, 34149 Trieste, Italy
    Nano Lett 12:4503-7. 2012
    ..We follow the reaction steps by X-ray photoemission spectroscopy and demonstrate the electrical insulation using a nanoscale multipoint probe technique...
  38. ncbi Kinetic control of catalytic CVD for high-quality graphene at low temperatures
    Robert S Weatherup
    Department of Engineering, University of Cambridge, Cambridge CB3 0FA, UK
    ACS Nano 6:9996-10003. 2012
    ..This also enables a route to the controlled formation of Bernal stacked bi- and few-layered graphene. The model is relevant to all catalyst materials and can readily serve as a general process rationale for optimized graphene CVD...
  39. ncbi Synthesis of oxidation-resistant cupronickel nanowires for transparent conducting nanowire networks
    Aaron R Rathmell
    Department of Chemistry, Duke University, 124 Science Drive, Box 90354 Durham, North Carolina 27708, United States
    Nano Lett 12:3193-9. 2012
    ..These properties, and the fact that copper and nickel are 1000 times more abundant than indium or silver, make cupronickel nanowires a promising alternative for the sustainable, efficient production of transparent conductors...
  40. ncbi Antifreeze protein from freeze-tolerant grass has a beta-roll fold with an irregularly structured ice-binding site
    Adam J Middleton
    Department of Biomedical and Molecular Sciences, Queen s University, Kingston, Ontario, Canada K7L 3N6
    J Mol Biol 416:713-24. 2012
    ....
  41. ncbi Oxygen switching of the epitaxial graphene-metal interaction
    Rosanna Larciprete
    CNR Institute for Complex Systems, Via Fosso del Cavaliere 100, 00133 Roma, Italy
    ACS Nano 6:9551-8. 2012
    ....
  42. pmc High-throughput protein purification and quality assessment for crystallization
    Youngchang Kim
    Midwest Center for Structural Genomics, Biosciences Division, Argonne National Laboratory, Argonne, IL 60439, USA
    Methods 55:12-28. 2011
    ....
  43. ncbi Oxygen-aided synthesis of polycrystalline graphene on silicon dioxide substrates
    Jianyi Chen
    Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China
    J Am Chem Soc 133:17548-51. 2011
    ..The method avoids the need for either a metal catalyst or a complicated and skilled postgrowth transfer process and is compatible with current silicon processing techniques...
  44. ncbi Advanced protein crystallization using water-soluble ionic liquids as crystallization additives
    Dariusch Hekmat
    Institute of Biochemical Engineering, Munich University of Technology, Boltzmannstr 15, 85748, Garching, Germany
    Biotechnol Lett 29:1703-11. 2007
    ..of five water-soluble, halogen-free, alkylammonium-based ionic liquids (ILs) as additives for advanced crystallization of lysozyme was investigated...
  45. ncbi On the mechanisms involved in biological heme crystallization
    Renata Stiebler
    Instituto de Bioquimica Medica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil
    J Bioenerg Biomembr 43:93-9. 2011
    ..One of these adaptations is the crystallization of heme into the dark-brown pigment hemozoin (Hz)...
  46. ncbi Role of renal epithelial cells in the initiation of calcium oxalate stones
    Saeed R Khan
    Department of Pathology, College of Medicine, University of Florida, Gainesville 32610 0275, USA
    Nephron Exp Nephrol 98:e55-60. 2004
    Normal urinary environment is inhibitory to crystallization. Occasional crystals are internalized by the renal epithelial cells and sequestered to lysosomes or externalized into the interstitium to be handled by the inflammatory cells...
  47. pmc Optimized purification of a heterodimeric ABC transporter in a highly stable form amenable to 2-D crystallization
    Carmen Galián
    Universite Joseph Fourier, Institut de Biologie Structurale, Grenoble, France
    PLoS ONE 6:e19677. 2011
    ..Electron microscopy of negatively stained crystals allowed the calculation of a projection map at 20 Å resolution revealing that BmrC/BmrD might assemble into oligomers in a lipidic environment...
  48. ncbi Oriented attachment and mesocrystals: non-classical crystallization mechanisms based on nanoparticle assembly
    Markus Niederberger
    Max Planck Institute of Colloids and Interfaces, Colloid Chemistry, Research Campus Golm, 14424, Potsdam, Germany
    Phys Chem Chem Phys 8:3271-87. 2006
    In this review, we highlight particle based crystallization pathways leading to single crystals via mesoscopic transformation...
  49. ncbi GPCR engineering yields high-resolution structural insights into beta2-adrenergic receptor function
    Daniel M Rosenbaum
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Science 318:1266-73. 2007
    ..To overcome the structural flexibility of the beta2AR and to facilitate its crystallization, we engineered a beta2AR fusion protein in which T4 lysozyme (T4L) replaces most of the third intracellular ..
  50. ncbi Cooperation of phosphates and carboxylates controls calcium oxalate crystallization in ultrafiltered urine
    Bernd Grohe
    Department of Dentistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, N6A 5C1, Canada
    Urol Res 39:327-38. 2011
    ..To this end, crystallization was induced by addition of CaOx solution to ultrafiltered human urine containing either human kidney OPN (..
  51. pmc Antioxidant properties of polysaccharide from the brown seaweed Sargassum graminifolium (Turn.), and its effects on calcium oxalate crystallization
    Chao Yan Zhang
    Shanghai Key Laboratory of Functional Materials Chemistry, Research Center of Analysis and Testing, East China University of Science and Technology, Shanghai 200237, China
    Mar Drugs 10:119-30. 2012
    ..SGP) on calcium oxalate crystallization, and determined its antioxidant activities...
  52. ncbi Organic-organic heteroepitaxy of red-, green-, and blue-emitting nanofibers
    Clemens Simbrunner
    Institute of Semiconductor and Solid State Physics, Johannes Kepler University Linz, Austria
    ACS Nano 4:6244-50. 2010
    ..Triband emission in the red, green, and blue is generated in nanofibers with thicker sexithiophene coverage, where layers of co-oriented crystals are separated by green-emitting molecular sheets...
  53. pmc Understanding the physical properties that control protein crystallization by analysis of large-scale experimental data
    W Nicholson Price
    Northeast Structural Genomics Consortium, Columbia University, New York, New York 10027, USA
    Nat Biotechnol 27:51-7. 2009
    b>Crystallization is the most serious bottleneck in high-throughput protein-structure determination by diffraction methods...
  54. ncbi Effects of crystallization of polyhydroxyalkanoate blend on surface physicochemical properties and interactions with rabbit articular cartilage chondrocytes
    Zhong Zheng
    Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China
    Biomaterials 26:3537-48. 2005
    ..In this study, PHBHHx was found to be completely miscible with PHB in their blends. Crystallization behavior of these polyester blends played an important role in determination of the surface physicochemical ..
  55. ncbi Counterdiffusion methods for macromolecular crystallization
    Juan Ma García-Ruiz
    Laboratorio de Estudios Cristalografiocos, CSIC University of Granada, Instituto Andaluz de Ciencias de la Tierra, 18002 Granada, Spain
    Methods Enzymol 368:130-54. 2003
  56. pmc DNA A-tract bending in three dimensions: solving the dA4T4 vs. dT4A4 conundrum
    Richard Stefl
    National Center for Biomolecular Research, NMR Laboratory, Faculty of Science, Masaryk University, Kotlarska 2, CZ 611 37 Brno, Czech Republic
    Proc Natl Acad Sci U S A 101:1177-82. 2004
    ..Results of gel electrophoretic mobility studies are consistent with the higher-order structure of the DNA and furthermore depend on the nature of the monovalent cation present in the running buffer...
  57. ncbi Crystal structure of the S-adenosylmethionine synthetase ternary complex: a novel catalytic mechanism of S-adenosylmethionine synthesis from ATP and Met
    Junichi Komoto
    Department of Molecular Biosciences, University of Kansas, 1200 Sunnyside Avenue, Lawrence, Kansas 66045 7534, USA
    Biochemistry 43:1821-31. 2004
    ..Pajares, M. A., Hermoso, J. A., Guillerm, D., Guillerm, G., and Sanz-Aparicio. J. (2003) J. Mol. Biol. 331, 407] is substantially different from our mechanism...
  58. ncbi Gold nanocages: bioconjugation and their potential use as optical imaging contrast agents
    Jingyi Chen
    Department of Chemistry, Department of Bioengineering, Department of Chemical Engineering, Division of Gastroenterology, Department of Medicine, University of Washington, Seattle, Washington 98195, USA
    Nano Lett 5:473-7. 2005
    ..26 x 10(-15) m2, suggesting their potential use as a new class of contrast agents for optical imaging. When bioconjugated with antibodies, the gold nanocages have also been demonstrated for specific targeting of breast cancer cells...
  59. pmc Structural basis for calcium sensing by GCaMP2
    Qi Wang
    Department of Molecular Medicine, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Structure 16:1817-27. 2008
    ..These studies provide a structural basis for the rational design of improved Ca(2+)-sensitive probes...
  60. ncbi Overexpression, purification and crystallization of bacteriocin LlpA from Pseudomonas sp. BW11M1
    Annabel H A Parret
    Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, 3001 Heverlee, Belgium
    Acta Crystallogr D Biol Crystallogr 60:1922-4. 2004
    ..Single crystals were grown by vapour diffusion and belong to space group P2(1)2(1)2, with unit-cell parameters a = 150.5, b = 154.5, c = 34.2 A. The crystals diffract to at least 2.2 A using synchrotron radiation...
  61. ncbi Photonic crystal nanostructures for optical biosensing applications
    D Dorfner
    Walter Schottky Institut and Physik Department, Technische Universitat Munchen, Am Coulombwall 3, D 85748 Garching, Germany
    Biosens Bioelectron 24:3688-92. 2009
    ..A response of partial delta lambda/delta c=(4.54+/-0.66)x10(5)nm/M is measured leading to a measured detection limit as good as Delta m=4.0+/-0.6 fg or Delta m/Delta A=(4.9+/-0.7)x10(2)pg/mm(2)in the sensitive area...
  62. ncbi Construction of highly ordered lamellar nanostructures through Langmuir-Blodgett deposition of molecularly thin titania nanosheets tens of micrometers wide and their excellent dielectric properties
    Kosho Akatsuka
    International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1 1 Namiki, Tsukuba, Ibaraki 305 0044, Japan
    ACS Nano 3:1097-106. 2009
    ..The obtained films showed superior dielectric and insulating properties as a reflection of the highly organized film nanoarchitecture...
  63. ncbi The initial stages of template-controlled CaCO3 formation revealed by cryo-TEM
    Emilie M Pouget
    Laboratory of Materials and Interface Chemistry, Eindhoven University of Technology, P O Box 513, 5600 MB Eindhoven, Netherlands
    Science 323:1455-8. 2009
    ..Our findings have implications for template-directed mineral formation in biological as well as in synthetic systems...
  64. ncbi Wafer scale homogeneous bilayer graphene films by chemical vapor deposition
    Seunghyun Lee
    Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, Michigan 48109, United States
    Nano Lett 10:4702-7. 2010
    ..The homogeneity of the film is further supported by electrical transport measurements on dual-gate bilayer graphene transistors, in which a band gap opening is observed in 98% of the devices...
  65. ncbi Oxidative renal cell injury induced by calcium oxalate crystal and renoprotection with antioxidants: a possible role of oxidative stress in nephrolithiasis
    Mauricio Davalos
    Department of Urology, New York Medical College, Valhalla, New York 10595, USA
    J Endourol 24:339-45. 2010
    ..We thus investigated if CaOx crystal would induce such renal cell injury in vitro and also explored how it would be carried out...
  66. ncbi Exfoliation and characterization of bismuth telluride atomic quintuples and quasi-two-dimensional crystals
    Desalegne Teweldebrhan
    Department of Electrical Engineering and Materials Science, Bourns College of Engineering, University of California Riverside, Riverside, California 92521, USA
    Nano Lett 10:1209-18. 2010
    ..The developed technology for producing free-standing quasi-2D layers of Te((1))-Bi-Te((2))-Bi-Te((1)) creates an impetus for investigation of the topological insulators and their possible practical applications...
  67. ncbi Nanostructured lipid matrices for improved microencapsulation of drugs
    R H Muller
    Department of Pharmaceutics, Biopharmaceutics and Biotechnology, Free University of Berlin, Kelchstr 31, 12169, Berlin, Germany
    Int J Pharm 242:121-8. 2002
    ..A special preparation process-applicable to NLC but also SLN-allows the production of highly concentrated particle dispersions (>30-95%). Potential applications as drug delivery system are described...
  68. ncbi Annexin 5 overexpression increased articular chondrocyte apoptosis induced by basic calcium phosphate crystals
    H K Ea
    INSERM U606, IFR 139, Hopital Lariboisiere, Paris, France
    Ann Rheum Dis 67:1617-25. 2008
    ..We assessed in vitro whether BCP crystals and overexpression of A5 increased chondrocyte apoptosis...
  69. pmc Toward rational protein crystallization: A Web server for the design of crystallizable protein variants
    Lukasz Goldschmidt
    Howard Hughes Medical Institute, University of California, Los Angeles DOE Institute of Genomics and Proteomics, Los Angeles, California 90095 1570, USA
    Protein Sci 16:1569-76. 2007
    Growing well-diffracting crystals constitutes a serious bottleneck in structural biology. A recently proposed crystallization methodology for "stubborn crystallizers" is to engineer surface sequence variants designed to form ..
  70. ncbi Structure of the complex of the colicin E2 R-domain and its BtuB receptor. The outer membrane colicin translocon
    Onkar Sharma
    Department of Biological Sciences, Lilly Hall of Life Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Biol Chem 282:23163-70. 2007
    ..Arg(452) at the R/C-domain interface in colicin E2 was found have an essential role at a putative site of protease cleavage, which would liberate the C-terminal activity domain for passage through the outer membrane translocon...
  71. ncbi Multiple functional groups of varying ratios in metal-organic frameworks
    Hexiang Deng
    California NanoSystems Institute, University of California Los Angeles UCLA Department of Energy DOE Institute of Genomics and Proteomics, Department of Chemistry and Biochemistry, UCLA, 607 Charles E Young Drive East, Los Angeles, CA 90095, USA
    Science 327:846-50. 2010
    ..For example, a member of this series, MTV-MOF-5-EHI, exhibits up to 400% better selectivity for carbon dioxide over carbon monoxide compared with its best same-link counterparts...
  72. ncbi Octacalcium phosphate crystals induce inflammation in vivo through interleukin-1 but independent of the NLRP3 inflammasome in mice
    Sharmal Narayan
    Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland
    Arthritis Rheum 63:422-33. 2011
    ..To determine the mechanisms involved in inflammatory responses to octacalcium phosphate (OCP) crystals in vivo...
  73. doi Tailoring the atomic structure of graphene nanoribbons by scanning tunnelling microscope lithography
    Levente Tapasztó
    Nat Nanotechnol 3:397-401. 2008
    ..This method avoids the difficulties of assembling nanoscale components and may prove useful in the realization of complete integrated circuits, operating as room-temperature ballistic electronic devices...
  74. ncbi Introduction to protein crystallization
    Alexander McPherson
    Department of Molecular Biology and Biochemistry, University of Califonia, Irvine 560 Steinhaus Hall, Irvine, CA 92697 3900, USA
    Methods 34:254-65. 2004
    ..The fundamental approaches to devising successful crystallization conditions and the factors that influence them are summarized here...
  75. ncbi Decreased inhibitory activity of prothrombin to calcium oxalate crystallization by specific chemical modification of its gamma-carboxyglutamic acid residues
    Jihong Liu
    Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
    Urology 67:201-3. 2006
    ..To investigate the inhibitory role of gamma (gamma)-carboxyglutamic acid (Gla) in the Gla domain of urinary prothrombin in the formation of calcium oxalate crystals...
  76. ncbi Dual modes of modification of hepatitis A virus 3C protease by a serine-derived beta-lactone: selective crystallization and formation of a functional catalytic triad in the active site
    Jiang Yin
    CIHR Group in Protein Structure and Function, Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    J Mol Biol 354:854-71. 2005
    ..However, crystallization of an enzyme-inhibitor adduct from the reaction mixture followed by X-ray structural analysis shows only ..
  77. ncbi Basic calcium phosphate crystals induce monocyte/macrophage IL-1β secretion through the NLRP3 inflammasome in vitro
    Borbála Pazár
    Department of Rheumatology, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland
    J Immunol 186:2495-502. 2011
    ..Furthermore, we speculate that IL-1 blockade could be a novel strategy to inhibit BCP-induced inflammation in human disease...
  78. pmc Nonlinear optical imaging of integral membrane protein crystals in lipidic mesophases
    David J Kissick
    Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA
    Anal Chem 82:491-7. 2010
    ..High turbidity is a hallmark of membrane protein crystallization due to the extensive use of detergent and/or lipids that often form various mesophases...
  79. pmc Self-catalyzed growth of S layers via an amorphous-to-crystalline transition limited by folding kinetics
    Sungwook Chung
    Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 107:16536-41. 2010
    The importance of nonclassical, multistage crystallization pathways is increasingly evident from theoretical studies on colloidal systems and experimental investigations of proteins and biomineral phases...
  80. ncbi Nanosized and nanocrystalline calcium orthophosphates
    Sergey V Dorozhkin
    Kudrinskaja sq 1 155, Moscow 123242, Russia
    Acta Biomater 6:715-34. 2010
    ..The review also provides possible directions for future research and development...
  81. pmc Silver-based crystalline nanoparticles, microbially fabricated
    T Klaus
    Department of Materials Science, The Angstrom Laboratory, Uppsala University, SE 751 21 Uppsala, Sweden
    Proc Natl Acad Sci U S A 96:13611-4. 1999
    ..These Ag-containing crystals are embedded in the organic matrix of the bacteria. Their possible potential as organic-metal composites in thin film and surface coating technology is discussed...
  82. ncbi Designed ankyrin repeat protein binders for the crystallization of AcrB: plasticity of the dominant interface
    Nicole Monroe
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    J Struct Biol 174:269-81. 2011
    ..One approach to improve crystal quality is the use of DARPins as crystallization chaperones...
  83. ncbi Prediction of TiO2 nanoparticle phase and shape transitions controlled by surface chemistry
    A S Barnard
    Center for Nanoscale Materials, Materials Science Division, Argonne National Laboratory, 9700 South Cass Ave, Argonne, Illinois 60439, USA
    Nano Lett 5:1261-6. 2005
    ..The latter introduces the possibility of inducing phase transitions by changing the surface chemistry...
  84. ncbi An overview of vertebrate mineralization with emphasis on collagen-mineral interaction
    W J Landis
    Department of Biochemistry and Molecular Pathology, Northeastern Ohio Universities College of Medicine, Rootstown 44272, USA
    Gravit Space Biol Bull 12:15-26. 1999
    ..In a broader sense, the data speak to the response of bone and mineralized vertebrate tissues to changes in gravitational loading and applied mechanical forces in general...
  85. ncbi Viral evolution revealed by bacteriophage PRD1 and human adenovirus coat protein structures
    S D Benson
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Cell 98:825-33. 1999
    ..Although these two dsDNA viruses infect hosts from very different kingdoms, their striking similarities, from major coat protein through capsid architecture, strongly suggest their evolutionary relationship...
  86. ncbi A novel extrapallial fluid protein controls the morphology of nacre lamellae in the pearl oyster, Pinctada fucata
    Zhuojun Ma
    Institute of Marine Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, 100084, China
    J Biol Chem 282:23253-63. 2007
    ..Our results suggest that a "safeguard mechanism" of undesired crystal growth is necessary for shell microstructure formation...
  87. ncbi A lipidic-sponge phase screen for membrane protein crystallization
    Annemarie B Wöhri
    Department of Chemical and Biological Engineering, Molecular Biotechnology, Chalmers University of Technology, SE 405 30 Gothenburg, Sweden
    Structure 16:1003-9. 2008
    ..To help address this problem, we have developed a sparse matrix crystallization screen consisting of 48 lipidic-sponge phase conditions...
  88. pmc The Scottish Structural Proteomics Facility: targets, methods and outputs
    Muse Oke
    Biomedical Sciences Research Complex, University of St Andrews, St Andrews, KY16 9ST, UK
    J Struct Funct Genomics 11:167-80. 2010
    ..By reporting the experimental details and depositing the structures we hope to maximize the impact of the project by allowing others to follow up the relevant biology...
  89. ncbi XtalPred: a web server for prediction of protein crystallizability
    Lukasz Slabinski
    Joint Center for Structural Genomics, La Jolla, CA 92037, USA
    Bioinformatics 23:3403-5. 2007
    ..with distributions of these features in TargetDB and combining the results into an overall probability of crystallization. XtalPred provides: (1) a detailed comparison of the protein's features to the corresponding distribution ..
  90. ncbi Fungus-mediated biotransformation of amorphous silica in rice husk to nanocrystalline silica
    Vipul Bansal
    Nanoscience Group, Materials Chemistry Division, and Biochemical Sciences Division, National Chemical Laboratory, Pune 411 008, India
    J Am Chem Soc 128:14059-66. 2006
    ....
  91. ncbi Corneal crystals in nephropathic cystinosis: natural history and treatment with cysteamine eyedrops
    W A Gahl
    Section on Human Biochemical Genetics, Heritable Disorders Branch, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    Mol Genet Metab 71:100-20. 2000
    ..Administration of 0.55% cysteamine eyedrops, given 6 to 12 times per day, dissolved corneal cystine crystals in 10 representative patients with nephropathic cystinosis aged 1 to 32 years within 8 to 41 months...
  92. ncbi Extracellular biosynthesis of magnetite using fungi
    Atul Bharde
    Nanoscience Group, Materials Chemistry Division, National Chemical Laboratory, Pune, India
    Small 2:135-41. 2006
    ....
  93. ncbi Phase determination using halide ions
    Miroslawa Dauter
    Macromolecular Crystallography Laboratory, National Cancer Institute, Argonne National Laboratory, Argonne, IL, USA
    Methods Mol Biol 364:149-58. 2007
    ..The halide cryosoaking approach involves very little preparative effort and offers a rapid and simple way of solving novel protein crystal structures...
  94. ncbi Crystallizing membrane proteins for structure determination: use of lipidic mesophases
    Martin Caffrey
    University of Limerick, Limerick, Ireland
    Annu Rev Biophys 38:29-51. 2009
    ..A hypothesis for how the method works at the molecular level and experimental evidence in support of the proposal are reviewed here. The latest advances, successes, and challenges associated with the method are described...
  95. ncbi Functionalized graphene sheets for polymer nanocomposites
    T Ramanathan
    Department of Mechanical Engineering, Northwestern University, Evanston, Illinois 60208, USA
    Nat Nanotechnol 3:327-31. 2008
    ..Modulus, ultimate strength and thermal stability follow a similar trend, with values for functionalized graphene sheet- poly(methyl methacrylate) rivaling those for single-walled carbon nanotube-poly(methyl methacrylate) composites...
  96. ncbi Role of macromolecular assembly of enamel matrix proteins in enamel formation
    H C Margolis
    Department of Biomineralization, The Forsyth Institute, 140 The Fenway, Boston, MA 02115, USA
    J Dent Res 85:775-93. 2006
    ..In conclusion, the authors present a hypothesis, based on the evidence presented, that the full-length amelogenin uniquely regulates proper enamel formation through a process of cooperative mineralization, and not as a pre-formed matrix...
  97. pmc Structure and substrate recruitment of the human spindle checkpoint kinase Bub1
    Jungseog Kang
    Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Mol Cell 32:394-405. 2008
    ..The KEN boxes of Bub1 are required for the spindle checkpoint in human cells. Therefore, its unusual active-site conformation and mode of substrate recruitment suggest that Bub1 has an exquisitely tuned specificity for Cdc20...
  98. pmc Statistical analysis of interface similarity in crystals of homologous proteins
    Qifang Xu
    Institute for Cancer Research, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA 19111, USA
    J Mol Biol 381:487-507. 2008
    ..The PDB, in particular, is missing highly likely biological interfaces in its biological unit files for about 10% of PDB entries...
  99. ncbi Equilibrium cluster formation in concentrated protein solutions and colloids
    Anna Stradner
    Department of Physics, University of Fribourg, Chemin du Musee 3, CH 1700 Fribourg, Switzerland
    Nature 432:492-5. 2004
    ..We discuss the relevance of this finding for nucleation processes during protein crystallization, protein or DNA self-assembly and the previously observed formation of cluster and gel phases in colloidal ..
  100. pmc Structural characterization of apical membrane antigen 1 (AMA1) from Toxoplasma gondii
    Joanna Crawford
    Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia V8W 3P6, Canada
    J Biol Chem 285:15644-52. 2010
    ..The detailed structural characterization of TgAMA1 provides valuable insight into the mechanism of host cell invasion by T. gondii...
  101. ncbi Role of S100A8 and S100A9 in neutrophil recruitment in response to monosodium urate monohydrate crystals in the air-pouch model of acute gouty arthritis
    Carle Ryckman
    Infectious Diseases Research Center, Centre de Recherche du Centre Hospitalier de l Universite Laval, 2705 Laurier Boulevard, Sainte Foy, Quebec G1V 4G2, Canada
    Arthritis Rheum 48:2310-20. 2003
    ..To examine the role of chemokines, S100A8, and S100A9 in neutrophil accumulation induced by the causative agent of gout, monosodium urate monohydrate (MSU) crystals...

Research Grants82

  1. Structure and function of urea transporters
    Ming Zhou; Fiscal Year: 2013
    ..3 [unreadable] resolution. We will further refine crystallization conditions to improve the resolution. Aim 2: To investigate mechanism of urea transport through dvUT...
  2. Identification and Analysis of Flavivirus Protease and RNA Helicase Inhibitors
    Radhakrishnan Padmanabhan; Fiscal Year: 2009
    ..This proposed research is likely lead to identification of novel inhibitors that will be useful for co-crystallization and structure-function studies that could provide valuable insight into the nature of molecular interactions ..
  3. Regulation of NFkappaB activity by the Estrogen Receptor
    Kendall W Nettles; Fiscal Year: 2012
    ..We recently developed a novel technique that accelerates the crystallization of the nuclear receptor ligand-binding domain by several orders of magnitude, allowing us to characterize ..
  4. Structural Studies of RNA Maturation and Processing
    Christopher D Lima; Fiscal Year: 2013
    ..b>Crystallization trials will be conducted with the long-term goal of illuminating the structural basis for TRAMP mediated ..
  5. Ligand Interaction of Glucagon Receptor by Structural and Functional Analyses
    FAI YIU SIU; Fiscal Year: 2012
    ..were solved with technological breakthroughs in baculovirus protein expression, receptor stabilization, and crystallization in cholesterol doped lipid cubic phase...
  6. Problems in Membrane Protein Crystallography: Hetero-Oligomeric Cytochrome b6f
    William A Cramer; Fiscal Year: 2012
    ..0 E. Studies on crystallization of the dimeric 220 kDa eight subunit integral b6f complex would analyze problems of proteolysis and lipid-..
  7. The bioorganic chemistry of RNA editing by ADARs
    Peter A Beal; Fiscal Year: 2013
    ..Finally, this project will produce new molecules for crystallization trials of ADAR-RNA complexes.
  8. Study of the HFE Transferrin Receptor interaction in mammalian iron homeostasis
    Paul Schmidt; Fiscal Year: 2010
    ..Previous studies have investigated the HFE/TFR interaction interface by co-crystallization of HFE and TFR1, by site-directed mutagenesis and by in vitro binding studies...
  9. Structure and Specificity in Metal Ion Homeostasis
    Amy C Rosenzweig; Fiscal Year: 2013
    ..The last specific aim is crystallization and structure determination of intact P1B-ATPases using several innovative approaches...
  10. Respiratory and Metabolic Adaptation to Hypoxia
    Paul T Schumacker; Fiscal Year: 2013
    ..This aim will be tested by protein crystallization studies of intact Complex III under different O2 concentrations...
  11. PSMA-based Cancer Imaging Agents
    Martin G Pomper; Fiscal Year: 2013
    ..actual imaging agents already synthesized and tested in vivo - as well as on molecular modeling, including co-crystallization of several of our existing agents with PSMA...
  12. FTIR STUDY OF SIGNAL TRANSDUCTION IN SENSORY RHODOPSINS
    Kenneth J Rothschild; Fiscal Year: 2010
    ..In the case of other SRs, even less information is known due to difficulties of crystallization and expression...
  13. Focal Adhesion Kinase - Tumor Biology and Therapeutics
    William G Cance; Fiscal Year: 2013
    ..We will study the binding of C4 to FAK by ITC and co-crystallization analyses...
  14. BIOCHEMISTRY AND PHYSIOLOGY OF PEPTIDE AMIDATION
    ELIZABETH ANNE EIPPER; Fiscal Year: 2013
    ..Roles for the linker regions connecting PHM and PAL will be explored in soluble PAM proteins with the goal of crystallization. This knowledge will clarify how the linkers affect the ability of membrane PAM to signal to cytosol and ..
  15. smFRET Investigation of Gating in a Neurotransmitter Transporter Homolog
    RACHEL ANN KOLSTER; Fiscal Year: 2013
    ..While the purification and crystallization of human NSS proteins has yet to be achieved, prokaryotic homologues have proven powerful model systems for ..
  16. Structural studies of PKR regulation by viral non-coding RNA
    GRAEME LESLIE CONN; Fiscal Year: 2013
    ..non-coding adenoviral transcript VA RNAI to produce a diverse 'library'of novel RNA constructs for crystallization with PKR...
  17. Transport and Inhibition in a Biogenic Amine Transporter
    Satinder Singh; Fiscal Year: 2009
    ..biogenic amine transporters in heterologous expression systems for monodispersity and their propensity for crystallization. A representative member will then be crystallized, and its structure solved...
  18. Transport and Inhibition in a Biogenic Amine Transporter
    SATINDER KAUR SINGH; Fiscal Year: 2012
    ..biogenic amine transporters in heterologous expression systems for monodispersity and their propensity for crystallization. A representative member will then be crystallized, and its structure solved...
  19. Synthetic Fabs for Crystallization of Chemokine:Receptor Complexes
    Tracy M Handel; Fiscal Year: 2013
    ..Despite these breakthroughs, GPCR structural biology remains non- trivial, particularly with respect to crystallization of complexes with other proteins and in relevant functional states...
  20. Structure of sclerostin protein complexes
    Rajiv Kumar; Fiscal Year: 2012
    ..the molecular mechanism of sclerostin interaction with Cyr61 C-terminal domain and in Aim 3 we will identify crystallization conditions for the sclerostin complex with LRP5 extracellular domain...
  21. Structure and mechanism of the dynein motor
    Ronald D Vale; Fiscal Year: 2013
    ..in the dynein field is the lack of atomic resolution structural information of its motor domain, and crystallization has been difficult to achieve because its very large size (>300 kDa)...
  22. Mechanisms of Metal Ion Regulation in Human Cells
    Svetlana Lutsenko; Fiscal Year: 2013
    ..the molecular mechanisms of copper uptake and export will be investigated using a combination of protein crystallization, electron microscopy, X-ray absorption spectroscopy as well as functional analysis of molecules involved in ..
  23. DEVELOPING SOLUTES AS STRUCTURAL/MECHANISTIC PROBES OF PROTEIN-DNA INTERACTIONS
    M Thomas Record; Fiscal Year: 2013
    ..g. protein crystallization, protein-nucleic acid interactions) will also be studied...
  24. High Throughput Crystallization / Imaging System
    Lawrence J Delucas; Fiscal Year: 2013
    ..Alabama at Birmingham (UAB): the availability of high throughput crystal imaging systems that can accommodate crystallization at the two most common temperatures, 20 [unreadable]C and 4 [unreadable]C...
  25. Biogenic Amine Transporters: Mechanisms of Ligand Interaction
    MAARTEN E REITH; Fiscal Year: 2013
    ..Structural insight into these proteins has recently come from crystallization of a bacterial homolog, the leucine transporter LeuT, showing the location of the substrate binding site (..
  26. Structure and Finction of AMPA subtype ionotropic glutamate receptors
    ALEXANDER SOBOLEVSKY; Fiscal Year: 2013
    ..We will use different crystallization methods and temperatures, screen detergents, lipids and ligands to obtain full length AMPA receptor ..
  27. Ceramic scaffolds with engineered topography and chemistry
    ISABELLE L DENRY; Fiscal Year: 2013
    ..We will first investigate the crystallization kinetics and mechanical properties of sintered niobium- doped fluorapatite (FAp) ceramics with the aim of ..
  28. Targeting DNA Secondary Structures for Bcl-2 Gene Regulation
    Danzhou Yang; Fiscal Year: 2013
    ..tool for determination of DNA secondary structures under physiological conditions, due to the difficulty of crystallization of such structures...
  29. ENERGY TRANSDUCTION IN CYTOCHROME OXIDASE
    SHELAGH M FERGUSON-MILLER; Fiscal Year: 2013
    ..aging, through structure/function analysis and the discovery of compounds that are physiological effectors, crystallization aids, mechanistic probes, or precursors to drugs that can modulate oxidase activity...
  30. Novel Therapeutics for Class B Protozoa
    Sharon L Reed; Fiscal Year: 2012
    ..EhCP1, one of two cysteine proteinases unique to E. histolytica, is undergoing crystallization studies under Dr. Van Voorhis...
  31. A lipid-based approach towards a high-resolution structure of a functional nAChR
    Jose Lasalde-Dominicci; Fiscal Year: 2013
    ..For the past several decades, the selection of detergents for membrane protein solubilization and/or crystallization has been a random experiment...
  32. Molecular basis for the regulation of G protein-coupled receptor kinases
    John J G Tesmer; Fiscal Year: 2012
    ..N-terminal helices of GRKs that bind with high affinity to activated bovine or cephalopod rhodopsin for co-crystallization screens...
  33. Chaperone Assisted Crystallography
    Anthony A Kossiakoff; Fiscal Year: 2012
    ..The CAC methodology is based on the use of synthetically derived antibody fragments as "crystallization chaperones" that specifically and tightly bind to a target protein or RNA entity and thereby promote ..
  34. Architectural Basis of Leptin Transmembrane Signaling
    Georgios Skiniotis; Fiscal Year: 2013
    ..characterization of dynamic protein assemblies in relatively small concentrations and without the need for crystallization. We anticipate that the application of single-particle EM techniques on this system will reveal the ..
  35. High-throughput Pipeline for Electron Crystallography
    David L Stokes; Fiscal Year: 2013
    ..with a high density of purified membrane proteins, thus providing a native membrane environment and fewer crystallization constraints for the constituent proteins...
  36. Structural and functional studies of the nuclear receptor PPARgamma
    H Eric Xu; Fiscal Year: 2010
    ..Our specific aims are focused on the crystallization and structural determination of 1) the PPAR? LBD bound to LNO2, which will provide the first view of PPAR? ..
  37. Structural Biology of G-Protein Coupled Receptors
    Wayne A Hendrickson; Fiscal Year: 2011
    ..cloning, purification, biochemical characterization, complexation with stabilizing ligands, and ultimate crystallization of relevant GPCRs will be developed as required...
  38. Chaperone-Enabled studies of epigenetic regulation enzymes
    Anthony A Kossiakoff; Fiscal Year: 2013
    ..generate specialized reagents called "synthetic affinity binders" or sABs that will be used as chaperones for crystallization, as well as customized affinity reagents for cell biologically applications...
  39. Crystallization Robot for the Structural Biology Resource Center at Rockefeller
    DEENA ABELLS OREN; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): This proposal requests funds to purchase a robot to set up crystallization experiments for the Structural Biology Resource Center at The Rockefeller University...
  40. Discovery of antibodies that bind G protein-coupled receptors
    Matthew P DeLisa; Fiscal Year: 2012
    ..and biology of most GPCRs remains elusive due to difficulties in protein expression, purification and crystallization, as well as the general lack of reagents for their manipulation in native environments...
  41. T cell responses to CD1-restricted lipids in tuberculosis
    DAVID BRANCH MOODY; Fiscal Year: 2013
    ..Further, biophysical measurements and protein crystallization will determine the strength and mechanisms of how lipid antigens contact T cell receptors...
  42. Diffraction Methods in Molecular Biology Gordon Research Conference
    NANCY RYAN GRAY; Fiscal Year: 2010
    ..for the presentation and discussion of recent advances in all aspects of diffraction methods, ranging from crystallization to model building and refinement...
  43. OSU Crystallography Facility: Acquisition of Crystallization Robot
    Michael K Chan; Fiscal Year: 2010
    ..for strength in this important research area, a major limitation is the absence of an automated system for crystallization. X-ray crystallography is an extremely powerful tool, but it requires that the sample molecule or complex of ..
  44. Production &Crystallization of Membrane Protein for 3D Structure
    Lawrence J Delucas; Fiscal Year: 2013
    ..Abstract: This proposal addresses challenges in eukaryotic protein expression, solublization, stablization and crystallization. We will accomplish this by integrating early assessment measurements of protein quality and quantity into an ..
  45. Developing Myxovirus Inhibitors with Expanded Pathogen Target Range
    Richard K Plemper; Fiscal Year: 2013
    ..as innovative molecular probes to optimize a folding-competent L fragment identified in pilot studies for co- crystallization. Extraction of a molecular pharmacophore will enable the transformation of this virus-specific scaffold to a ..
  46. Crystallization of outer membrane proteins for export of polysaccharide capsule
    Mark A Saper; Fiscal Year: 2012
    ..Pure protein will be used to screen for crystallization conditions...
  47. Crystallization and structure determination of the mu-opioid receptor
    Roger K Sunahara; Fiscal Year: 2012
    ..with T4L not only stabilized the receptor but it also contributed a nicely ordered and solvent-accessible crystallization packing surface...
  48. Ca2+ Transport Mechanism of CaCA Protein Family
    Lei Zheng; Fiscal Year: 2013
    ..crystals diffracting to 6 [unreadable] resolution, and have designed innovative approaches to optimize the crystallization for structure determination by x-ray crystallography...
  49. Electron Microscopy of P-type Ion Pumps
    David L Stokes; Fiscal Year: 2010
    ..For FxYD proteins, we will develop conditions for 2D crystallization Na/K-ATPase and phospholemman after heterologous expression and purification from yeast.
  50. Molecular Mechanism of Allosteric Modulation of the Oxytocin Receptor by Sterols
    VADIM GENNADYEVICH CHEREZOV; Fiscal Year: 2013
    ..successful strategy of replacing the third intracellular loop of the receptor with T4 lysozyme combined with crystallization in LCP, which has recently yielded high resolution structures of human b2 adrenergic and adenosine A2A GPCRs...
  51. Lipid Regulation of Thrombin Generation
    Barry R Lentz; Fiscal Year: 2012
    ..We have cross-linked a Xa-Va complex assembled by C6PS and will determine if it would be appropriate for crystallization trials...
  52. Viral Constraints on SIV Escape from Cytotoxic T Lymphocytes
    WENDY WEN LI YEH; Fiscal Year: 2010
    ..relevance of the epitope and flanking mutations on viral core formation using electron microscopy and x-ray crystallization. In addition, we will evaluate the pathogenicity and immunological consequences of the escape variant SHIV-89...
  53. Structure and Functions of Steroid Hormone Receptors
    H Eric Xu; Fiscal Year: 2010
    ..Our specific aims are focused on crystallization and structural determination of 1) the MR LBD, 2) the GR LBD in complex with cortivazol, a potent anti-..
  54. In Situ Sensing of Single Myosin Function in Hypertrophy Disease
    Thomas P Burghardt; Fiscal Year: 2013
    ..RLC crystallization and structure determination will investigate the structural basis of RLC regulation of myosin as well as the ..
  55. Genetic Optimization of Membrane Protein Production for Crystallization
    Mark E Dumont; Fiscal Year: 2012
    ..of TMPs leading to heterogeneity and loss of native conformations during expression, purification, and crystallization for x-ray diffraction...
  56. HDL prevention of cholesterol crystal inflammation in HIV disease
    Eicke Latz; Fiscal Year: 2013
    ..to the ability of HDL to prevent cholesterol crystal formation we will;1) Establish that cholesterol crystallization is proportional to plasma VLDL/LDL levels in the LDLR-/- mouse model of atherosclerosis using a series of ..
  57. Crystallization of eukaryotic facilitated glucose transporters
    Lan Guan; Fiscal Year: 2009
    ..This proposal will focus on the crystallization of eukaryotic facilitated Gluts, particularly Glut1 from human RBCs...
  58. Regulation of heparanase in cancer progression
    Israel Vlodavsky; Fiscal Year: 2013
    ..e., crystallization and structural analysis) to carry out and accomplish each of the proposed specific aims.
  59. Structural investigation of a GABA receptor subtype
    DEREK CLAXTON; Fiscal Year: 2013
    ..Then optimized constructs will be expressed in Sf9 insect cells to obtain mg quantities of receptor for crystallization trials...
  60. Structural characterization of a human neurotransmitter transporter
    Kevin H Wang; Fiscal Year: 2012
    ..This application details strategies to express and purify a human neurotransmitter transporter for crystallization and structural determination via x- ray diffraction...
  61. LIGHT-DRIVEN ION TRANSPORT IN BACTERIAL RHODOPSINS
    Janos K Lanyi; Fiscal Year: 2013
    ..absorption and fluorescence), site-specific spin labeling, Fourier Transform Infrared spectroscopy, crystallization and x-ray diffraction, and solution NMR...
  62. A. gambiae thioester-containing protein 1 (TEP1) protein-protein interactions
    Richard H G Baxter; Fiscal Year: 2011
    ..Baxter's doctoral degree is in the field of chemistry, but his research involves the purification and crystallization of proteins, therefore he has both the rigorous physical training and the appropriate laboratory skills to ..
  63. ANESTHETIC MECHANISMS AND GABA-A RECEPTOR STRUCTURE
    MYLES AKABAS; Fiscal Year: 2001
    ..The crystallization of integral membrane proteins has been a difficult problem and it is unlikely that a crystal structure of the ..
  64. Nanoliter Lab-on-a-chip for Protein Crystallization
    Vamsee Pamula; Fiscal Year: 2007
    ..b>Crystallization and X-ray diffraction of these proteins is routinely performed to determine their 3D structure which needs to ..
  65. Structural Studies of HBsAg: Support for Quentin Florence
    QUENTIN FLORENCE; Fiscal Year: 2009
    ..in the initial HBsAg sample characterization (protein purity, homnogenity, etc) and in setting up the initial crystallization screens at the University of Georgia (using locally developed screens) and at the high-throughput screening ..
  66. Targets for Drug Design In Flaviviral Infection
    Kurt Krause; Fiscal Year: 2004
    ..and express sufficient quantities of NS3A, NS2B and NS3B from West Nile virus and Yellow Fever virus for crystallization trials. These proteins represent the viral protease, its activating peptide and the viral helicase...
  67. 3-D CRYSTALLIZATION OF MEMBRANE PROTEINS
    JAMES GOUAUX; Fiscal Year: 1999
    ..coli complex is an ideal model system. The specific aims are to test new detergents for crystallization, to prepare Fv fragments which bind to tertiary epitopes on the membrane protein complexes, to carry out ..
  68. Crystallization of His-tag Proteins on Nanostructured 1D & 2D Template Interface
    David Thompson; Fiscal Year: 2007
    ..new nitrilotriacetic acid (NTA) reagents that promote nucleation in a symmetry-guided manner and accelerate crystallization via phase separation into IMP-rich domains...
  69. Anachem Lipidic Cubic Phase Crystallization Robot
    Robert M Stroud; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Membrane Protein Crystallization and Structure Determination in Lipidic Cubic Phase or Sponge Mesophase: Membrane proteins constitute 30% of the genome, and are targets for over 40% of all drugs in ..
  70. MOLECULAR ICE CONTROL FOR ENGINEERED TISSUES
    Michael Taylor; Fiscal Year: 2003
    ..Cryopreservation would provide an ideal solution to this need except that ice crystallization in complex multicomponent tissues causes injury to the cells and the extracellular matrix rendering the ..
  71. STRUCTURE OF BACTERIAL MEMBRANE PROTEINS
    R Garavito; Fiscal Year: 1992
    The crystallization of transmembrane channel-forming proteins (porins) from the outer membrane of enteric (gram negative) bacteria (e.g., E...
  72. Efficient Synthesis of Se-DNAs and Se-RNAs for Structure and Function Studies
    Zhen Huang; Fiscal Year: 2010
    ..However, the crystallization and heavy atom derivatization for phase determination have largely slowed down structural determination of ..
  73. Nanostructured Block-copolymer Reinforced Calcium Phosphate Composites
    LARA ESTROFF; Fiscal Year: 2009
    ..3) Controlled crystallization of the amorphous, inorganic phase of the nanostructured composites...
  74. Application of solution NMR to (human) membrane cytochrome P450 17A1
    DAVID FERNANDO ESTRADA; Fiscal Year: 2012
    ..little of the enzymes'behavior in solution or with ligands that do not form tight complexes amenable to crystallization. Therefore, important structural aspects of human P450 enzyme function have not been monitored in solution...
  75. CRYSTALLIZATION OF AN ABC TRANSPORTER
    BING JAP; Fiscal Year: 1999
    ..The current proposal is to determine the crystallization conditions of maltose transporter in order to produce crystals that are suitable for subsequent ..
  76. Innovative Methods for Membrane Protein Crystallization
    PAUL KENIS; Fiscal Year: 2006
    This proposal focuses on the creation and validation of novel crystallization platforms and methodology to substantially speed up the process of determining optimal crystallization conditions for membrane proteins...
  77. STRUCTURE OF MICROSOMAL ENZYMES--ESTROGEN BIOSYN
    Debashis Ghosh; Fiscal Year: 2000
    ..The major objective of this proposed research is crystallization of two membrane-bound naturally-occurring, full-length, fully-active human enzymes P450arom and ES, in order ..
  78. CRYSTALLIZATION OF AN RNA-PROTEIN COMPLEX
    Ming Luo; Fiscal Year: 2001
    ..The results from these experiments will lead to the crystallization of a unique protein-RNA complex that produces high resolution diffraction data...
  79. Membrane Protein Structure by Electron Crystallography
    David Stokes; Fiscal Year: 2009
    ..Nevertheless, the proliferation of high- throughput screening for 3D crystallization trials has given a distinct advantage to X-ray crystallography...
  80. MOLECULAR ORGANIZATION OF ION CHANNELS--A NOVEL APPROACH
    Anatoli Lopatin; Fiscal Year: 1999
    ..This crystallization method will be further developed to reveal unknown aspects of molecular organization and function of ..
  81. A cholesterol biosynthetic yeast for mammalian membrane protein production
    NIALL FRASER; Fiscal Year: 2007
    ..important, mammalian membrane proteins is obtaining sufficient pure, functional protein for sustained crystallization trials. Yeasts are commonly used for the heterologous production of soluble recombinant proteins...
  82. CALCIUM OXALATE IN BIOLOGICAL SYSTEMS
    ROSS HOLMES; Fiscal Year: 1999
    ..function and metabolism, and clinical scientists who examine the pathophysiology of calcium oxalate crystallization, particularly in the formation of kidney stones...