high pressure liquid chromatography

Summary

Summary: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.

Top Publications

  1. ncbi Mechanistic investigation of ionization suppression in electrospray ionization
    R King
    Department of Drug Metabolism, West Point, Pennsylvania 19468, USA
    J Am Soc Mass Spectrom 11:942-50. 2000
  2. ncbi A new metabolic pathway of arsenite: arsenic-glutathione complexes are substrates for human arsenic methyltransferase Cyt19
    Toru Hayakawa
    Environmental Health Sciences Division, National Institute for Environmental Studies, 16 2 Onogawa, Tsukuba, 305 8506, Ibaraki, Japan
    Arch Toxicol 79:183-91. 2005
  3. pmc Comparative quantitative trait loci mapping of aliphatic, indolic and benzylic glucosinolate production in Arabidopsis thaliana leaves and seeds
    D J Kliebenstein
    Max Planck Institute for Chemical Ecology, 07745 Jena, Germany
    Genetics 159:359-70. 2001
  4. pmc Reproducibility of urinary phthalate metabolites in first morning urine samples
    Jane A Hoppin
    Epidemiology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709 2233, USA
    Environ Health Perspect 110:515-8. 2002
  5. pmc Gene duplication in the diversification of secondary metabolism: tandem 2-oxoglutarate-dependent dioxygenases control glucosinolate biosynthesis in Arabidopsis
    D J Kliebenstein
    Max Planck Institute for Chemical Ecology, 07745 Jena, Germany
    Plant Cell 13:681-93. 2001
  6. ncbi Composition and content of glucosinolates in developing Arabidopsis thaliana
    Bent Larsen Petersen
    Plant Biochemistry Laboratory, Department of Plant Biology, Royal Veterinary and Agricultural University, Frederiksberg C, Copenhagen, Denmark
    Planta 214:562-71. 2002
  7. ncbi Evaluation of multidimensional chromatography coupled with tandem mass spectrometry (LC/LC-MS/MS) for large-scale protein analysis: the yeast proteome
    Junmin Peng
    Department of Cell Biology, and Taplin Biological Mass Spectrometry Facility, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    J Proteome Res 2:43-50. 2003
  8. pmc Isolation of the yeast nuclear pore complex
    M P Rout
    Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021
    J Cell Biol 123:771-83. 1993
  9. pmc Epithelins 1 and 2: isolation and characterization of two cysteine-rich growth-modulating proteins
    M Shoyab
    Oncogen, Seattle, WA 98121
    Proc Natl Acad Sci U S A 87:7912-6. 1990
  10. ncbi A conserved family of enzymes that phosphorylate inositol hexakisphosphate
    Sashidhar Mulugu
    Howard Hughes Medical Institute, Department of Pharmacology and Cancer Biology, Duke University Medical Center, DUMC 3813, Durham, NC 27710, USA
    Science 316:106-9. 2007

Detail Information

Publications292 found, 100 shown here

  1. ncbi Mechanistic investigation of ionization suppression in electrospray ionization
    R King
    Department of Drug Metabolism, West Point, Pennsylvania 19468, USA
    J Am Soc Mass Spectrom 11:942-50. 2000
    ..The results point to changes in the droplet solution properties caused by the presence of nonvolatile solutes as the main cause of ionization suppression in electrospray ionization of biological extracts...
  2. ncbi A new metabolic pathway of arsenite: arsenic-glutathione complexes are substrates for human arsenic methyltransferase Cyt19
    Toru Hayakawa
    Environmental Health Sciences Division, National Institute for Environmental Studies, 16 2 Onogawa, Tsukuba, 305 8506, Ibaraki, Japan
    Arch Toxicol 79:183-91. 2005
    ..Metabolism of iAsIII to methylated arsenicals by Cyt19 was via ATG and MADG rather than by oxidative methylation of iAsIII and MMA(III)...
  3. pmc Comparative quantitative trait loci mapping of aliphatic, indolic and benzylic glucosinolate production in Arabidopsis thaliana leaves and seeds
    D J Kliebenstein
    Max Planck Institute for Chemical Ecology, 07745 Jena, Germany
    Genetics 159:359-70. 2001
    ..These data show that there are a large number of variable loci controlling glucosinolate accumulation in Arabidopsis thaliana...
  4. pmc Reproducibility of urinary phthalate metabolites in first morning urine samples
    Jane A Hoppin
    Epidemiology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709 2233, USA
    Environ Health Perspect 110:515-8. 2002
    ..5 for mBzP. These results suggest that even with the short half-lives of phthalates, women's patterns of exposure may be sufficiently stable to assign an exposure level based on a single first morning void urine measurement...
  5. pmc Gene duplication in the diversification of secondary metabolism: tandem 2-oxoglutarate-dependent dioxygenases control glucosinolate biosynthesis in Arabidopsis
    D J Kliebenstein
    Max Planck Institute for Chemical Ecology, 07745 Jena, Germany
    Plant Cell 13:681-93. 2001
    ..A third member of this gene family, AOP1, is present in at least two forms and found in all ecotypes examined. However, its catalytic role is still uncertain...
  6. ncbi Composition and content of glucosinolates in developing Arabidopsis thaliana
    Bent Larsen Petersen
    Plant Biochemistry Laboratory, Department of Plant Biology, Royal Veterinary and Agricultural University, Frederiksberg C, Copenhagen, Denmark
    Planta 214:562-71. 2002
    ..This indicates that there is a continuous turnover of glucosinolates during development and not only upon tissue disruption...
  7. ncbi Evaluation of multidimensional chromatography coupled with tandem mass spectrometry (LC/LC-MS/MS) for large-scale protein analysis: the yeast proteome
    Junmin Peng
    Department of Cell Biology, and Taplin Biological Mass Spectrometry Facility, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    J Proteome Res 2:43-50. 2003
    ..New criteria are proposed to decrease false-positives to less than 1% and to greatly reduce the need for manual interpretation while permitting more proteins to be identified...
  8. pmc Isolation of the yeast nuclear pore complex
    M P Rout
    Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021
    J Cell Biol 123:771-83. 1993
    ..It retains all but one of the eight known NPC proteins. In addition it contains as many as 80 uncharacterized proteins that are candidate NPC proteins...
  9. pmc Epithelins 1 and 2: isolation and characterization of two cysteine-rich growth-modulating proteins
    M Shoyab
    Oncogen, Seattle, WA 98121
    Proc Natl Acad Sci U S A 87:7912-6. 1990
    ..However, a significant homology was seen between the amino-terminal sequences of epithelin 1 and epithelin 2. Epithelins 1 and 2, therefore, appear to represent members of a distinct family of growth regulators...
  10. ncbi A conserved family of enzymes that phosphorylate inositol hexakisphosphate
    Sashidhar Mulugu
    Howard Hughes Medical Institute, Department of Pharmacology and Cancer Biology, Duke University Medical Center, DUMC 3813, Durham, NC 27710, USA
    Science 316:106-9. 2007
    ..Alterations in kinase activity led to defects in cell growth, morphology, and interactions with ARP complex members. The functionality of Asp1 and Vip1 may provide cells with increased signaling capacity through metabolism of IP6...
  11. ncbi Genetic control of melatonin synthesis in the pineal gland of the mouse
    S Ebihara
    Science 231:491-3. 1986
    ..Pineal glands from C57BL/6J mice have neither NAT nor HIOMT activity. These results suggest that the two genes involved in melatonin deficiency are responsible for the absence of normal NAT and HIOMT enzyme activity...
  12. ncbi Rapid screening of the entire mitochondrial DNA for low-level heteroplasmic mutations
    David Meierhofer
    Department of Paediatrics, Paracelsus Private Medical University Salzburg, Muellner Hauptstr 48, A 5020 Salzburg, Austria
    Mitochondrion 5:282-96. 2005
    ..In order to demonstrate that the method has diagnostic value, we analyzed and confirmed known mtDNA mutations in patient samples...
  13. ncbi Disruption of methylarginine metabolism impairs vascular homeostasis
    James Leiper
    Centre for Clinical Pharmacology, Division of Medicine, University College London, London WC1E 6JJ, UK
    Nat Med 13:198-203. 2007
    ..Our results also suggest that DDAH inhibition could be harnessed therapeutically to reduce the vascular collapse associated with sepsis...
  14. pmc Familial deficiency of dihydropyrimidine dehydrogenase. Biochemical basis for familial pyrimidinemia and severe 5-fluorouracil-induced toxicity
    R B Diasio
    Division of Clinical Pharmacology, University of Alabama, Birmingham 35294
    J Clin Invest 81:47-51. 1988
    ..Patients who are deficient in this enzyme are likely to develop severe toxicity after FUra administration...
  15. ncbi In vivo MHC class II presentation of cytosolic proteins revealed by rapid automated tandem mass spectrometry and functional analyses
    A R Dongre
    Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle 98195, USA
    Eur J Immunol 31:1485-94. 2001
    ..These results suggest that central tolerance to at least some MHC class II-bound self peptides derived from cytosolic proteins exists in vivo...
  16. pmc Mutation detection in the ABCC6 gene and genotype-phenotype analysis in a large international case series affected by pseudoxanthoma elasticum
    Ellen G Pfendner
    GeneDx Inc, 207 Perry Parkway, Gaithersburg, Maryland 20877, USA
    J Med Genet 44:621-8. 2007
    ..It is caused by mutations in the ABCC6 (ATP binding cassette family C member 6) gene, which encodes MRP6 (multidrug resistance-associated protein 6)...
  17. ncbi CYP98A3 from Arabidopsis thaliana is a 3'-hydroxylase of phenolic esters, a missing link in the phenylpropanoid pathway
    G Schoch
    Department of Plant Stress Response, Institute of Plant Molecular Biology, CNRS UPR 2357, 28 rue Goethe, F 67083 Strasbourg cedex, France
    J Biol Chem 276:36566-74. 2001
    ..This hydroxylation occurs on depsides, the function of which was so far not understood, revealing an additional and unexpected level of networking in lignin biosynthesis...
  18. ncbi Practical implementation of 2D HPLC scheme with accurate peptide retention prediction in both dimensions for high-throughput bottom-up proteomics
    Ravi C Dwivedi
    Manitoba Centre for Proteomics and Systems Biology, University of Manitoba, 799 JBRC, 715 McDermot Avenue, Winnipeg, MB, R3E 3P4, Canada
    Anal Chem 80:7036-42. 2008
    ....
  19. ncbi Insect immunity. Constitutive expression of a cysteine-rich antifungal and a linear antibacterial peptide in a termite insect
    M Lamberty
    , , CNRS, , , 67084 Strasbourg Cedex, France
    J Biol Chem 276:4085-92. 2001
    ....
  20. ncbi Automated on-line column-switching HPLC-MS/MS method with peak focusing for the determination of nine environmental phenols in urine
    Xiaoyun Ye
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA
    Anal Chem 77:5407-13. 2005
    ..The method can be used for quick and accurate analysis of large numbers of samples in epidemiologic studies for assessing the prevalence of human exposure to environmental phenols...
  21. ncbi Antioxidant activity and phenolic compounds in selected herbs
    W Zheng
    Fruit Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, U. S. Department of Agriculture, Beltsville, Maryland 20705, USA
    J Agric Food Chem 49:5165-70. 2001
    ..longiflora, whereas quercetin-3-O-rhamnosyl-(1 --> 2)-rhamnosyl-(1 --> 6)-glucoside and kaempferol-3-O-rhamnosyl-(1 --> 2)-rhamnosyl-(1 --> 6)-glucoside were predominant phenolic compounds in Ginkgo biloba leaves...
  22. pmc Improved peptide identification in proteomics by two consecutive stages of mass spectrometric fragmentation
    Jesper V Olsen
    Center for Experimental Bioinformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    Proc Natl Acad Sci U S A 101:13417-22. 2004
    ..When MS(3) is performed on the linear ion trap-Fourier transform mass spectrometer combination, accurate peptide masses further increase confidence in peptide identification...
  23. pmc Determination of monomethylarsonous acid, a key arsenic methylation intermediate, in human urine
    X C Le
    University of Alberta, Department of Public Health Sciences, Edmonton, Alberta, Canada
    Environ Health Perspect 108:1015-8. 2000
    ..Along with the presence of MMA(III), there was an increase in the relative concentration of MMA(V) and a decrease in DMA(V) in the urine samples collected after the DMPS ingestion...
  24. ncbi Simultaneous quantification of 14 ginsenosides in Panax ginseng C.A. Meyer (Korean red ginseng) by HPLC-ELSD and its application to quality control
    Su Na Kim
    Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 110 460, South Korea
    J Pharm Biomed Anal 45:164-70. 2007
    ..The amounts of the 14 ginsenosides in the various ginseng samples could be analyzed simultaneously. This validated HPLC method is expected to provide a new basis for the quality assessment of ginseng products...
  25. pmc Effects of interferon-alpha on rhesus monkeys: a nonhuman primate model of cytokine-induced depression
    Jennifer C Felger
    Department of Psychiatry and Behavioral Sciences, Emory University School of Medicine, Winship Cancer Institute, 1365 C Clifton Road, Atlanta, GA 30322, USA
    Biol Psychiatry 62:1324-33. 2007
    ..To establish a nonhuman primate model of cytokine-induced depression, we examined the effects of IFN-alpha on rhesus monkeys...
  26. pmc Large-scale proteomic analysis of the human spliceosome
    Juri Rappsilber
    Protein Interaction Laboratory in the Center of Experimental Bioinformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense M, Denmark
    Genome Res 12:1231-45. 2002
    ..This investigation provides the most detailed inventory of human spliceosome-associated factors to date, and the data indicate a number of interesting links coordinating splicing with other steps in the gene expression pathway...
  27. ncbi Botanical origin and chemical composition of Brazilian propolis
    Yong K Park
    Department of Food Science, College of Food Engineering, State University of Campinas, 13081 970 Campinas, Sao Paulo, Brazil
    J Agric Food Chem 50:2502-6. 2002
    ..It was concluded that the origins of propolis group 3, group 6, and group 12 are resins of the poplar tree, Hyptis divaricata, and Baccharis dracunculifolia, respectively...
  28. ncbi Shaken, not stirred: mechanical stress testing of an IgG1 antibody
    Sylvia Kiese
    Formulation R and D Biologics, Pharmaceutical and Analytical R and D, F Hoffmann La Roche Ltd, Basel, Switzerland
    J Pharm Sci 97:4347-66. 2008
    ..An array of analytical methods was essential in order to cover the vast aggregate morphologies, which occurred during agitation...
  29. ncbi Trivalent arsenicals are bound to proteins during reductive methylation
    Hua Naranmandura
    Graduate School of Pharmaceutical Sciences, Chiba University, Chuo, Chiba 260 8675, Japan
    Chem Res Toxicol 19:1010-8. 2006
    ..We also discussed the in vitro formation of dimethylthioarsenicals after incubating dimethylarsinous acid with liver homogenate...
  30. pmc Concentrations of the sunscreen agent benzophenone-3 in residents of the United States: National Health and Nutrition Examination Survey 2003--2004
    Antonia M Calafat
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA
    Environ Health Perspect 116:893-7. 2008
    ..BP-3 has other uses in many consumer products (e.g., as fragrance and flavor enhancer, photoinitiator, ultraviolet curing agent, polymerization inhibitor)...
  31. ncbi Rapid dereplication of estrogenic compounds in pomegranate (Punica granatum) using on-line biochemical detection coupled to mass spectrometry
    Danny A van Elswijk
    Kiadis, Niels Bohrweg 11 13, 2333 AC, Leiden, The Netherlands
    Phytochemistry 65:233-41. 2004
    ....
  32. ncbi Characterization of the Escherichia coli SSB-113 mutant single-stranded DNA-binding protein. Cloning of the gene, DNA and protein sequence analysis, high pressure liquid chromatography peptide mapping, and DNA-binding studies
    J W Chase
    J Biol Chem 259:805-14. 1984
    ..Analysis of tryptic and chymotryptic peptides of the mutant protein by high pressure liquid chromatography and solid phase protein sequencing has shown that the ssb-113 mutation results in the substitution ..
  33. ncbi Tryptophan degradation increases with stage in patients with rheumatoid arthritis
    Katharina Schroecksnadel
    Division of Biological Chemistry, Biocentre Fritz, Innsbruck Medical University, Pregl Strasse 3, 6020 Innsbruck, Austria
    Clin Rheumatol 25:334-7. 2006
    ..It could also be relevant for the mood of patients, as tryptophan is the precursor of serotonin...
  34. pmc Widespread occurrence of glyceryl ether monooxygenase activity in rat tissues detected by a novel assay
    Ernst R Werner
    Division of Biological Chemistry, Biocenter, Innsbruck Medical University, A 6020 Innsbruck, Austria
    J Lipid Res 48:1422-7. 2007
    ....
  35. ncbi Chemical composition and biological activity of a new type of Brazilian propolis: red propolis
    S M Alencar
    Department of Agri Food industry, Food and Nutrition, Luiz de Queiroz College of Agriculture, University of Sao Paulo, Avenida Pádua Dias 11, Piracicaba, CEP 13418 900, SP, Brazil
    J Ethnopharmacol 113:278-83. 2007
    ..Our results showed that the red propolis has biologically active compounds that had never been reported in other types of Brazilian propolis...
  36. ncbi Effect of a new variety of Apis mellifera propolis on mutans Streptococci
    H Koo
    Department of Physiological Sciences, Faculty of Dentistry of Piracicaba, State University of Campinas, Caixa Postal 52, Piracicaba, 13414 900, SP, Brazil
    Curr Microbiol 41:192-6. 2000
    ....
  37. ncbi Ascorbic acid content of human corneal epithelium
    R F Brubaker
    Department of Ophthalmology, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55905, USA
    Invest Ophthalmol Vis Sci 41:1681-3. 2000
    ..To measure the concentration of ascorbic acid in the human corneal epithelium...
  38. pmc Kinetic flux profiling for quantitation of cellular metabolic fluxes
    Jie Yuan
    241 Carl Icahn Laboratory, Department of Chemistry and Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA
    Nat Protoc 3:1328-40. 2008
    ..This protocol is exemplified using E. coli and nitrogen labeling, for which quantitative flux data for approximately 15 metabolites can be obtained over 3 d of work. Applications to adherent mammalian cells are also discussed...
  39. ncbi Kynurenic acid levels are elevated in the cerebrospinal fluid of patients with schizophrenia
    S Erhardt
    Department of Physiology and Pharmacology, Karolinska Institute, 171 77 Stockholm, Sweden
    Neurosci Lett 313:96-8. 2001
    ..acid was analyzed in 28 male schizophrenic patients and 17 male healthy controls by means of high pressure liquid chromatography and fluorescence detection. Schizophrenic patients showed elevated CSF levels of kynurenic acid (1...
  40. ncbi Measurement of 18 perfluorinated organic acids and amides in human serum using on-line solid-phase extraction
    Zsuzsanna Kuklenyik
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA
    Anal Chem 77:6085-91. 2005
    ..The high throughput and low limits of detection (0.05-0.8 ng/mL) using a small sample volume (100 microL of serum) and isotope dilution quantification make this method suitable for large-scale epidemiologic studies...
  41. ncbi Combined use of MLPA and nonfluorescent multiplex PCR analysis by high performance liquid chromatography for the detection of genomic rearrangements
    Laura De Lellis
    Department of Oncology and Neurosciences, University G D Annunzio, Chieti, Italy
    Hum Mutat 27:1047-56. 2006
    ....
  42. ncbi Oxidative DNA damage associated with combination of guanine and superoxide radicals and repair mechanisms via radical trapping
    Richard Misiaszek
    Chemistry Department and Radiation and Solid State Laboratory, New York University, New York, New York 10003 5180, USA
    J Biol Chem 279:32106-15. 2004
    ..The mechanistic aspects of the radical reactions that either regenerate undamaged guanine in DNA or lead to oxidatively modified guanine bases are discussed...
  43. ncbi Flavonoid metabolites in human plasma and urine after the consumption of red onions: analysis by liquid chromatography with photodiode array and full scan tandem mass spectrometric detection
    William Mullen
    Plant Products and Human Nutrition Group, Graham Kerr Building, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
    J Chromatogr A 1058:163-8. 2004
    ..Despite a high dosage, neither anthocyanins nor anthocyanin metabolites accumulated in either plasma or urine in detectable quantities...
  44. pmc Increased striatal dopamine release and hyperdopaminergic-like behaviour in mice lacking both alpha-synuclein and gamma-synuclein
    Steven L Senior
    Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK
    Eur J Neurosci 27:947-57. 2008
    ..Rather, we propose that loss of both alpha- and gamma-synuclein causes an increase in release probability from dopaminergic synapses...
  45. ncbi Measurement of cholesterol of major serum lipoprotein classes by anion-exchange HPLC with perchlorate ion-containing eluent
    Yuji Hirowatari
    Scientific Instruments Division, TOSOH Corp, 2743 1 Hayakawa Ayase shi, Kanagawa 252 1123, Japan
    J Lipid Res 44:1404-12. 2003
    ..883 and r = 0.729, respectively). This rapid and accurate HPLC method was successfully applied to the analysis of plasma lipoproteins of patients with hyperlipidemia...
  46. ncbi Synthesis, structural characterization, and immunological properties of carbon nanotubes functionalized with peptides
    Davide Pantarotto
    Institute of Molecular and Cellular Biology, UPR 9021 CNRS, 67084 Strasbourg, France
    J Am Chem Soc 125:6160-4. 2003
    ..In addition, this peptide-NT conjugate is immunogenic, eliciting antibody responses of the right specificity. Such a system could be greatly advantageous for diagnostic purposes and could find future applications in vaccine delivery...
  47. ncbi Biallelic germline mutations in MYH predispose to multiple colorectal adenoma and somatic G:C-->T:A mutations
    Sian Jones
    Institute of Medical Genetics, University of Wales College of Medicine, Heath Park, Cardiff, CF14 4XN, UK
    Hum Mol Genet 11:2961-7. 2002
    ..These findings confirm the role of MYH in colorectal adenoma and carcinoma predisposition...
  48. ncbi Ion-pair reversed-phase high-performance liquid chromatography analysis of oligonucleotides: retention prediction
    Martin Gilar
    Waters Corporation, Milford, MA 01757, USA
    J Chromatogr A 958:167-82. 2002
    ..The TEA-HFIP aqueous buffers are useful for a highly efficient and less sequence-dependent separation of heterooligonucleotides...
  49. ncbi Quercetin is recovered in human plasma as conjugated derivatives which retain antioxidant properties
    C Manach
    Unité des Maladies Métaboliques et Micronutriments, I N R A de Clermont Ferrand Theix, St Genes Champanelle, France
    FEBS Lett 426:331-6. 1998
    ..3'-O-Methylquercetin and conjugated derivatives of quercetin significantly prolonged the lag phase, but the magnitude of their effect was about half that of the aglycone...
  50. ncbi Mutational analysis of the ABCC6 gene and the proximal ABCC6 gene promoter in German patients with pseudoxanthoma elasticum (PXE)
    Veronika Schulz
    Institut fur Laboratoriums und Transfusionsmedizin, Herz und Diabeteszentrum Nordrhein Westfalen, Universitatsklinik der Ruhr Universitat Bochum, Bad Oeynhausen, Germany
    Hum Mutat 27:831. 2006
    ..Obtaining these genetic data signifies our contribution to elucidating the pathogenetics of PXE...
  51. ncbi A scavenger function for a Drosophila peptidoglycan recognition protein
    Peter Mellroth
    Department of Microbiology, Stockholm University, S 106 91 Stockholm, Sweden
    J Biol Chem 278:7059-64. 2003
    ..Furthermore, a sequence homology comparison with phage T7 lysozyme, also an N-acetylmuramoyl-l-alanine amidase, shows that as many as six of the Drosophila PGRPs could belong to this class of proteins...
  52. ncbi General method for rapid synthesis of multicomponent peptide mixtures
    A Furka
    Department of Organic Chemistry, , Budapest, Hungary
    Int J Pept Protein Res 37:487-93. 1991
    ..Then the portion are mixed and before the next coupling cycle the resin is again distributed into equal portions. The method is illustrated by the synthesis of a mixture of 27 tetrapeptides and that of 180 pentapeptides...
  53. ncbi Extravascular transport of the DNA intercalator and topoisomerase poison N-[2-(Dimethylamino)ethyl]acridine-4-carboxamide (DACA): diffusion and metabolism in multicellular layers of tumor cells
    K O Hicks
    Auckland Cancer Society Research Centre, Faculty of Medicine and Health Sciences, The University of Auckland, Auckland, New Zealand
    J Pharmacol Exp Ther 297:1088-98. 2001
    ..In conclusion, this study demonstrates that it is possible to design DNA intercalators that diffuse efficiently in tumor tissue, and that there is little impediment to DACA transport over distances required for its antitumor action...
  54. ncbi Disposition of a low dose of 14C-bisphenol A in male rats and its main biliary excretion as BPA glucuronide
    Hideo Kurebayashi
    Division of Pharmacology, National Institute of Health Sciences, Kamiyoga 1 18 1, Setagaya, Tokyo 158 8501, Japan
    Toxicol Sci 73:17-25. 2003
    ..These results suggest that BPA is mainly metabolized to BPA-gluc and excreted into feces through the bile and subject to enterohepatic circulation in rats irrespective of dose and administration route...
  55. pmc New connections across pathways and cellular processes: industrialized mutant screening reveals novel associations between diverse phenotypes in Arabidopsis
    Yan Lu
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, USA
    Plant Physiol 146:1482-500. 2008
    ....
  56. ncbi Changes in neurotransmitter levels and proinflammatory cytokine mRNA expressions in the mice olfactory bulb following nanoparticle exposure
    - Tin-Tin-Win-Shwe
    National Institute for Environmental Studies, 16 2 Onogawa, Tsukuba, Ibaraki 305 8506, Japan
    Toxicol Appl Pharmacol 226:192-8. 2008
    ..These findings suggest that nanoparticle CB may modulate the extracellular amino acid neurotransmitter levels and proinflammatory cytokine IL-1 beta mRNA expressions synergistically with LTA in the mice olfactory bulb...
  57. ncbi Sequence-specific retention calculator. Algorithm for peptide retention prediction in ion-pair RP-HPLC: application to 300- and 100-A pore size C18 sorbents
    Oleg V Krokhin
    Manitoba Centre for Proteomics and Systems Biology, University of Manitoba, 799 JBRC, 715 McDermot Avenue, Winnipeg, MB, R3E 3P4, Canada
    Anal Chem 78:7785-95. 2006
    ..The SSRCalc algorithm has also been extensively tested for a number of real samples, providing solid support for protein identification and characterization; correlations in the range of 0.95-0.97 R2 value have normally been observed...
  58. pmc The nature of arsenic-phytochelatin complexes in Holcus lanatus and Pteris cretica
    Andrea Raab
    Department of Chemistry, University of Aberdeen, Meston Building, Meston Walk, Aberdeen AB24 3UE, United Kingdom
    Plant Physiol 134:1113-22. 2004
    ..In both plant species, As is dominantly in non-bound inorganic forms, with 13% being present in PC complexes for H. lanatus and 1% in P. cretica...
  59. ncbi Concentration of enzymatically active prostate-specific antigen (PSA) in the extracellular fluid of primary human prostate cancers and human prostate cancer xenograft models
    S R Denmeade
    The Johns Hopkins Oncology Center, Bunting Blaustein Cancer Research Building, Baltimore, Maryland 21231, USA
    Prostate 48:1-6. 2001
    ..Prostate-specific antigen (PSA) targeted prodrugs are under development in our laboratory. Concentrations of total PSA and enzymatically active PSA produced by various human prostate cancer xenograft models have not been well characterized...
  60. pmc HPLC/UV quantitation of retinal, retinol, and retinyl esters in serum and tissues
    MAUREEN A KANE
    Department of Nutritional Science and Toxicology, University of California, Berkeley, CA 94720, USA
    Anal Biochem 378:71-9. 2008
    ..The assays described here provide for sensitive and rigorous quantification of endogenous RE, ROL, and RAL to elucidate retinoid homeostasis in disease states such as Alzheimer's disease, type 2 diabetes, obesity, and cancer...
  61. ncbi Liquid- and gas-phase nitration of bovine serum albumin studied by LC-MS and LC-MS/MS using monolithic columns
    Wolfgang Walcher
    Institute of Analytical Chemistry and Radiochemistry, Leopold Franzens University, A 6020 Innsbruck, Austria
    J Proteome Res 2:534-42. 2003
    ..By use of such a model system, RP-HPLC--ESI--MS proved to be a rapid and highly efficient method for the comprehensive and quantitative detection of protein nitration...
  62. ncbi Exploring the in vitro formation of trimethylarsine sulfide from dimethylthioarsinic acid in anaerobic microflora of mouse cecum using HPLC-ICP-MS and HPLC-ESI-MS
    Kevin M Kubachka
    US EPA, ORD, NERL, Microbiological and Chemical Exposure, Assessment Research Division, Cincinnati, OH 45268, USA
    Toxicol Appl Pharmacol 239:137-43. 2009
    ..Therefore, 34S-TMAS(V) was incubated and the exchange was monitored with respect to time. The data suggest that the As-S bond associated with TMAS(V) is less labile than the As-S bond associated with DMTA(V)...
  63. ncbi Chemical forms of selenium for cancer prevention
    Rizky Abdulah
    Department of Public Health, Graduate School of Medicine, Gunma University, 3 39 22, Showa machi, Maebashi City, Gunma 371 8511, Japan
    J Trace Elem Med Biol 19:141-50. 2005
    ..g., Se-methylselenocysteine and methylseleninic acid), are more efficacious than other Se compounds because of their chemoprevention activity...
  64. ncbi 4'-phosphopantetheinyl transferase-encoding npgA is essential for siderophore biosynthesis in Aspergillus nidulans
    Harald Oberegger
    Department of Molecular Biology, University of Innsbruck, Peter Mayr Strasse 4b, 6020 Innsbruck, Austria
    Curr Genet 44:211-5. 2003
    ....
  65. ncbi Clinical pharmacokinetics of everolimus
    Gabriele I Kirchner
    Department of Gastroenterology, Hepatology and Endocrinology, Zentrum Innere Medizin, Medizinische Hochschule Hannover, Hannover, Germany
    Clin Pharmacokinet 43:83-95. 2004
    ..The target trough concentration of everolimus should range between 3 and 15 microg/L in combination therapy with ciclosporin (trough concentration 100-300 microg/L) and prednisone...
  66. ncbi Polyphenolic profiles in eight apple cultivars using high-performance liquid chromatography (HPLC)
    Rong Tsao
    Food Research Program, Agriculture and Agri Food Canada, 93 Stone Road West, Guelph, Ontario N1G 5C9, Canada
    J Agric Food Chem 51:6347-53. 2003
    ..3-Hydroxyphloretin 2'-xyloglucoside was newly identified in apple. The results obtained in this study will further the understanding of the polyphenolic composition of apples and their roles in health-promoting physiological functions...
  67. ncbi A gender-specific discriminator in Sprague-Dawley rat urine: the deployment of a metabolic profiling strategy for biomarker discovery and identification
    Mark P Hodson
    Safety Assessment Division, GlaxoSmithKline, Ware, UK
    Anal Biochem 362:182-92. 2007
    ..This approach will be deployed in future studies of disease, drug efficacy, and toxicity to discover and identify biologically relevant markers...
  68. ncbi Different techniques for urinary protein analysis of normal and lung cancer patients
    Payungsak Tantipaiboonwong
    Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan
    Proteomics 5:1140-9. 2005
    ..Therefore, there are alternative choices for urine sample preparation for studying the urinary proteome and identifying urinary protein markers important for further preclinical diagnostic and therapeutic applications...
  69. ncbi Cellular background level of 8-oxo-7,8-dihydro-2'-deoxyguanosine: an isotope based method to evaluate artefactual oxidation of DNA during its extraction and subsequent work-up
    Jean Luc Ravanat
    Laboratoire Lesions des Acides Nucleiques, DRFMC SCIB UMR 5046 CEA Grenoble, 17 Avenue des Martyrs, F 38054 Grenoble Cedex 9, France
    Carcinogenesis 23:1911-8. 2002
    ..Using the best-studied protocol, the steady-state cellular background level of 8-oxodGuo, in a lymphocyte cell line, was determined to be approximately 0.5 lesions/10(6) DNA nucleosides...
  70. ncbi Simultaneous analysis of the majority of low-molecular-weight, redox-active compounds from mitochondria
    B S Kristal
    Dementia Research Service, Burke Medical Research Institute, 785 Mamaroneck Avenue, White Plains, New York 10605, USA
    Anal Biochem 263:18-25. 1998
    ..Results, including analytical parameters, from a study of liver mitochondria from control and diabetic rats are presented to demonstrate utility of this methodology...
  71. ncbi Variation of glucosinolate accumulation among different organs and developmental stages of Arabidopsis thaliana
    Paul D Brown
    Department of Biochemistry, Max Planck Institute for Chemical Ecology, Winzerlaer Strasse 10, D 07745 Jena, Germany
    Phytochemistry 62:471-81. 2003
    ..During seed germination and leaf senescence, there were significant declines in glucosinolate concentration. The physiological and ecological significance of these findings is briefly discussed...
  72. ncbi Human metabolism of dietary flavonoids: identification of plasma metabolites of quercetin
    A J Day
    Institute of Food Research, Norwich, UK
    Free Radic Res 35:941-52. 2001
    ..The existence of substitutions in the B and/or C ring of plasma quercetin metabolites suggests that these conjugates will each have very different biological activities...
  73. ncbi HPLC-ESI-MS analysis of oral human fluids reveals that gingival crevicular fluid is the main source of oral thymosins beta(4) and beta(10)
    Rosanna Inzitari
    Istituto di Biochimica e di Biochimica Clinica, Università Cattolica and Istituto per la Chimica del Riconoscimento Molecolare, CNR, Istituto Scientifico Internazionale ISI Paolo VI, Roma, Italy
    J Sep Sci 32:57-63. 2009
    ..On the whole, results indicate that gingival sulcus is a main, although not the sole, source for oral Tbeta(4 )and Tbeta(10)...
  74. ncbi Quantitative analysis of SMN1 and SMN2 genes based on DHPLC: a highly efficient and reliable carrier-screening test
    Yi Ning Su
    Department of Medical Genetics, National Taiwan University Hospital, Taipei, Taiwan
    Hum Mutat 25:460-7. 2005
    ..We demonstrated that DHPLC is a fast and reliable tool for detection of carriers of SMA...
  75. ncbi HPLC method for 25-hydroxyvitamin D measurement: comparison with contemporary assays
    Gary L Lensmeyer
    Clinical Toxicology Laboratory, University of Wisconsin Hospital and Clinics, Department of Pathology, University of Wisconsin Madison, Madison, WI 53792, USA
    Clin Chem 52:1120-6. 2006
    ..Unfortunately, variability among 25(OH)D assays limits clinician ability to monitor VitD status, supplementation, and toxicity...
  76. ncbi Diurnal variation of arachidonoylethanolamine, palmitoylethanolamide and oleoylethanolamide in the brain of the rat
    Eric Murillo-Rodriguez
    Depto de Neurociencias, Instituto de Fisiologia Celular, Universidad Nacional Autonoma de Mexico, Mexico D F CP 04510, Mexico
    Life Sci 79:30-7. 2006
    ....
  77. pmc Urinary concentrations of triclosan in the U.S. population: 2003-2004
    Antonia M Calafat
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA
    Environ Health Perspect 116:303-7. 2008
    ..Triclosan is a synthetic chemical with broad antimicrobial activity that has been used extensively in consumer products, including personal care products, textiles, and plastic kitchenware...
  78. ncbi Method performance and multi-laboratory assessment of a normal phase high pressure liquid chromatography-fluorescence detection method for the quantitation of flavanols and procyanidins in cocoa and chocolate containing samples
    Rebecca J Robbins
    Analytical and Applied Sciences Group, Mars, Incorporated, 800 High Street Hackettstown, NJ 07840, USA
    J Chromatogr A 1216:4831-40. 2009
    ..Inter-lab assessment indicated that variability was quite low for seven different cocoa-containing samples, with a RSD(R) of less than 10% for the range of samples analyzed...
  79. ncbi Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes
    P Matsudaira
    J Biol Chem 262:10035-8. 1987
    ..The results suggest that PVDF membranes are superior supports for sequence analysis of picomole quantities of proteins purified by gel electrophoresis...
  80. ncbi Trypsin cleaves exclusively C-terminal to arginine and lysine residues
    Jesper V Olsen
    Center for Experimental Bioinformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    Mol Cell Proteomics 3:608-14. 2004
    ..Our results indicate that such peptide hits in previous studies should be re-examined and that peptide identification should be based on strict trypsin specificity...
  81. ncbi Thio-dimethylarsinate is a common metabolite in urine samples from arsenic-exposed women in Bangladesh
    Reingard Raml
    Karl Franzens University Graz, Institute of Chemistry Analytical Chemistry, Universitaetsplatz 1, 8010 Graz, Austria
    Toxicol Appl Pharmacol 222:374-80. 2007
    ..The work highlights the need for analytical methods providing specific determinations of arsenic compounds in future studies on arsenic metabolism and toxicology...
  82. ncbi Serum concentrations of 11 polyfluoroalkyl compounds in the u.s. population: data from the national health and nutrition examination survey (NHANES)
    Antonia M Calafat
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA
    Environ Sci Technol 41:2237-42. 2007
    ....
  83. ncbi Methylation of p16 CpG islands associated with malignant transformation of gastric dysplasia in a population-based study
    Yu Sun
    Peking University School of Oncology and Beijing Institute for Cancer Research, Beijing, People s Republic of China
    Clin Cancer Res 10:5087-93. 2004
    ..The aim of this study is to investigate the relationship between p16 methylation and malignant transformation of human gastric dysplasia (DYS) based on follow-up endoscopic screening in a high-risk population...
  84. ncbi Quantification of urinary conjugates of bisphenol A, 2,5-dichlorophenol, and 2-hydroxy-4-methoxybenzophenone in humans by online solid phase extraction-high performance liquid chromatography-tandem mass spectrometry
    Xiaoyun Ye
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Hwy, Mailstop F17, Atlanta, GA 30341, USA
    Anal Bioanal Chem 383:638-44. 2005
    ..To our knowledge, this is the first study describing the distribution of urinary conjugates of BP-3 and 2,5-DCP in humans...
  85. ncbi Alterations of the intestinal transport and processing of gliadin peptides in celiac disease
    Tamara Matysiak-Budnik
    INSERM EMI 0212, Faculté Necker Enfants Malades, Paris
    Gastroenterology 125:696-707. 2003
    ....
  86. ncbi Capillary hydrophilic interaction chromatography/mass spectrometry for simultaneous determination of multiple neurotransmitters in primate cerebral cortex
    Xiaozhe Zhang
    Max Plank Institute for Biological Cybernetics, 72076 Tubingen, Germany
    Rapid Commun Mass Spectrom 21:3621-8. 2007
    ..We conclude that HILIC/MS/MS combined with the push-pull sampling method represents a sensitive technique for simultaneous monitoring of neurotransmitters from EBF samples...
  87. ncbi A field survey of metal binding to metallothionein and other cytosolic ligands in liver of eels using an on-line isotope dilution method in combination with size exclusion (SE) high pressure liquid chromatography (HPLC) coupled to Inductively Coupled Plas
    Karen Van Campenhout
    Department of Biology, University of Antwerp, Groenenborgerlaan 171, B 2020 Antwerp, Belgium
    Sci Total Environ 394:379-89. 2008
    ..An on-line isotope dilution method in combination with size exclusion (SE) high pressure liquid chromatography (HPLC) coupled to Inductively Coupled Plasma time-of-flight Mass Spectrometry (ICP-TOFMS) was used ..
  88. ncbi Decreased serum levels of D-serine in patients with schizophrenia: evidence in support of the N-methyl-D-aspartate receptor hypofunction hypothesis of schizophrenia
    Kenji Hashimoto
    Department of Psychiatry, Graduate School of Medicine, Chiba University, Japan
    Arch Gen Psychiatry 60:572-6. 2003
    ..The aim of this study was to examine whether serum levels of D- and L-serine in patients with schizophrenia are different from those of healthy controls...
  89. ncbi GDP-mannose 3',5'-epimerase forms GDP-L-gulose, a putative intermediate for the de novo biosynthesis of vitamin C in plants
    Beata A Wolucka
    Department of Molecular Microbiology, Flanders Interuniversity Institute for Biotechnology VIB, Kasteelpark Arenberg 31, B 3001 Leuven Heverlee, Belgium
    J Biol Chem 278:47483-90. 2003
    ..Exogenous L-gulose and L-gulono-1,4-lactone serve as direct precursors of l-ascorbic acid in plant cells. We propose an L-gulose pathway for the de novo biosynthesis of vitamin C in plants...
  90. ncbi Protein-bound and free UV filters in cataract lenses. The concentration of UV filters is much lower than in normal lenses
    Anastasia Korlimbinis
    Save Sight Institute, University of Sydney, GPO Box 4337, Sydney, NSW 2001, Australia
    Exp Eye Res 85:219-25. 2007
    ....
  91. ncbi Human UDP-glucuronosyltransferase isoforms involved in bisphenol A glucuronidation
    Nobumitsu Hanioka
    Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan
    Chemosphere 74:33-6. 2008
    ..These findings demonstrate that BPA is mainly glucuronidated by UGT2B15 in human liver microsomes, and suggest that this UGT isoform plays important roles in the detoxification and elimination of BPA...
  92. ncbi Isolation and syntheses of polymethoxyflavones and hydroxylated polymethoxyflavones as inhibitors of HL-60 cell lines
    Shiming Li
    Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, NJ 08901 8520, USA
    Bioorg Med Chem 15:3381-9. 2007
    ....
  93. ncbi Mutation analysis of MLH1 and MSH2 genes performed by denaturing high-performance liquid chromatography
    Grzegorz Kurzawski
    Hereditary Cancer Center Department of Genetics and Pathology, Pomeranian Academy of Medicine, ul Polabska 4, 70 115, Szczecin, Poland
    J Biochem Biophys Methods 51:89-100. 2002
    ..DHPLC was confirmed to be highly sensitive, specific and a cost-effective technique with particularly high potential for the detection of MSH2 and MLH1 gene mutations in the diagnostic setting...
  94. ncbi Tryptophan-derived ultraviolet filter compounds covalently bound to lens proteins are photosensitizers of oxidative damage
    Jasminka Mizdrak
    Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia
    Free Radic Biol Med 44:1108-19. 2008
    ..These data indicate that the covalent modification of lens proteins by Kyn derivatives yields photosensitizers that may enhance oxidation in older lenses and contribute to age-related nuclear cataract...
  95. ncbi Primary structure of PDC-109, a major protein constituent of bovine seminal plasma
    F S Esch
    Biochem Biophys Res Commun 113:861-7. 1983
    ..The 12,774 dalton structure has two structurally similar domains of 38 and 41 amino acids, each containing two disulfide bonds...
  96. pmc Complete amino acid sequence of BSP-A3 from bovine seminal plasma. Homology to PDC-109 and to the collagen-binding domain of fibronectin
    N G Seidah
    Biochem J 243:195-203. 1987
    ..The collagen-binding domain of bovine fibronectin (type II sequence) was also found to be highly homologous to both BSP-A3 and PDC-109...
  97. ncbi Determination of coenzyme Q10 status in blood mononuclear cells, skeletal muscle, and plasma by HPLC with di-propoxy-coenzyme Q10 as an internal standard
    Andrew J Duncan
    Division of Neurochemistry, Institute of Neurology, London, UK
    Clin Chem 51:2380-2. 2005
  98. ncbi Evaluation of surface plasmon resonance relative to high pressure liquid chromatography for the determination of paralytic shellfish toxins
    Dorothea F K Rawn
    Food Research Division, Bureau of Chemical Safety, Health Products and Food Branch, Health Canada, Sir Frederick Banting Research Centre, Tunney s Pasture, Ottawa, ON, Canada K1A 0K9
    J Agric Food Chem 57:10022-31. 2009
    ..SPR may be applicable as a screening technique, although improved antibody response to the N-1-hydroxylated PSTs is required prior to this method being safely used for routine testing...
  99. ncbi Recent advances in high-throughput quantitative bioanalysis by LC-MS/MS
    Raymond Naxing Xu
    Abbott Laboratories, Department of Drug Analysis, 100 Abbott Park Road, Abbott Park, IL 60064 6126, USA
    J Pharm Biomed Anal 44:342-55. 2007
    ..In the end, this paper also discusses recent studies of matrix effect in LC-MS/MS analysis and how to reduce/eliminate matrix effect in method development and validation...
  100. ncbi Retention of paclitaxel in cancer cells for 1 week in vivo and in vitro
    Taisuke Mori
    Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji, Kamigyo ku, 602 8566, Kyoto, Japan
    Cancer Chemother Pharmacol 58:665-72. 2006
    ..Here, we investigate the pharmacokinetics and the pharmacodynamics of weekly paclitaxel in cancer cells in vivo and in vitro...
  101. pmc Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach
    Bryson D Bennett
    Department of Chemistry and Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA
    Nat Protoc 3:1299-311. 2008
    ..It enables absolute quantitation of several dozen metabolites over approximately 1 week of work...

Research Grants62

  1. Deriving hepatocytes from disease specific iPS to treat metabolic liver disorders
    Namita Roy-Chowdhury; Fiscal Year: 2013
    ....
  2. Heme Oxygenase Polymorphisms and Hepatopulmonary Syndrome
    KARI EVAN ROBERTS; Fiscal Year: 2012
    ..To define rare (1-10%) genetic variation in conserved segments of the H01 promoter using denaturing high pressure liquid chromatography, and then examine the association of this variation with HPS...
  3. Impact of Estrogens and Menopause on Interacting Monoamine Neurotransmitters in t
    Jeffrey K Yao; Fiscal Year: 2013
    ..The findings will provide a much clearer understanding of the neurochemical changes associated with different types of menopause and will lead to better strategies for approaching estrogen therapy in women. ..
  4. TARGETS OF AMINO ACID RESTRICTION IN PROSTATE CANCER
    Gary G Meadows; Fiscal Year: 2009
    ..Intracellular amino acids will be determined by high pressure liquid chromatography and apoptosis will be measured by flow cytometry...
  5. Regulation of DNA Methylation by a Major Folate Enzyme
    KYLE CRAIG STRICKLAND; Fiscal Year: 2012
    ..This project will characterize a metabolic process related to folic acid that may provide insight into the prevention or treatment of diabetic disease. ..
  6. Thermo Vantage triple quadrupole with FAIMS and Accela UPLC
    KYM FRANCIS FAULL; Fiscal Year: 2010
    ..triple quadrupole mass spectrometer (QqQ) and two essential ancillary attachments - an ultra-high pressure liquid chromatography (UPLC) system, and a High- Field Assymetric Waveform Ion Mobility Spectrometry (FAIMS) attachment...
  7. Neural Substrates of Phasic versus Sustained Fear
    David L Walker; Fiscal Year: 2013
    ..Recent studies indicate that the neural substrates of short-duration fear responses may be different from those of more sustained fear responses. The experiments proposed herein are meant to further explore these differences. ..
  8. Locus Coeruleus Function and Methylphenidate Action
    BARRY DALE WATERHOUSE; Fiscal Year: 2013
    ..and density of noradrenergic profiles across various brain regions, 3) microdialysis and high pressure liquid chromatography with electrochemical detection (HPLC-EC) of NE release in sensory and cognitive brain areas before ..
  9. Hydrodynamic Chirality: A novel method for chiral separation and analysis
    Osman Kibar; Fiscal Year: 2013
    ..Current techniques for chiral analysis and isolation such as high pressure liquid chromatography, x-ray crystallography and vibrational circular dichromism are frequently time consuming, expensive,..
  10. ABI Elite ESI-QqTOF Mass Spectrometry System
    Natalie G Ahn; Fiscal Year: 2010
    ..mass spectrometer capable of rapid scanning and high accuracy mass measurements, and a Waters ultra-high pressure liquid chromatography system for high resolution analyte separations...
  11. Corticostriatal networks and NMDAR mediation of habitual and flexible action
    Jonathan L Brigman; Fiscal Year: 2013
    ..chronic systemic drug administration as well as tissue micro-dissection for western blotting and high pressure liquid chromatography. In addition I have expanded his surgical techniques to include implantation of indwelling guide ..
  12. Role of Early B Cell Factor-1 (EBF-1) in Adipocyte Biology
    Michael J Griffin; Fiscal Year: 2010
    ....
  13. Smaller Particle Fused-Core Silicas for Higher Performance Separations
    Barry E Boyes; Fiscal Year: 2011
    ..PUBLIC HEALTH RELEVANCE: High pressure liquid chromatography is the most widely used analytical method to separate mixtures of molecules, allowing measurement ..
  14. Retinal degeneration diseases and the visual retinoid cycle
    Akiko Maeda; Fiscal Year: 2012
    ..mechanisms, efficacy and safety of our candidate drugs by biochemical methods such as HPLC (high pressure liquid chromatography) and mass-spectrometry, and electroretinography, and histological/histocytochemical assessment...
  15. SBIR TOPIC321:CHEMICALLY DEFINED GLYCAN LIBRARIES FOR REFERENCE STANDARDS AND GLY
    Qingfeng Pan; Fiscal Year: 2013
    ..These latter syntheses will be difficult to conduct without the knowledge, experience and technical know-how gained from the proposed Phase I work. ..
  16. PROTEOGLYCAN SYNTHESIS DURING DEVELOPMENT AND AGING
    Arnold Caplan; Fiscal Year: 1999
    ..Immunoreactive hexasaccharides, obtained by immuno-affinity procedures, will be fractionated by high pressure liquid chromatography into different subpopulations based upon their chemical structures...
  17. Predictive algorithms for T cell epitope identification
    Soren Buus; Fiscal Year: 2009
    ..limited to: computer-based epitope prediction algorithms;genome-wide scanning;structural genomic;high pressure liquid chromatography;mass spectrometry;phage-display libraries;and combinatorial synthetic peptide library screens...
  18. Direct Epitope Identification and Validation
    William Hildebrand; Fiscal Year: 2009
    ..limited to: computer-based epitope prediction algorithms;genome-wide scanning;structural genomic;high pressure liquid chromatography;mass spectrometry;phage-display libraries;and combinatorial synthetic peptide library screens...
  19. Large Scale Antibody and T Cell Epitope Discovery Program
    PH D FRELINGER; Fiscal Year: 2009
    ..limited to: computer-based epitope prediction algorithms;genome-wide scanning;structural genomic;high pressure liquid chromatography;mass spectrometry;phage-display libraries;and combinatorial synthetic peptide library screens...
  20. ROLE OF POLYAMINES IN POSTNATAL COCHLEAR DEVELOPMENT
    Charles Henley; Fiscal Year: 1993
    ....
  21. MECHANISM OF ACTION OF NEW ANTICANCER AGENTS
    WILLIAM CREASEY; Fiscal Year: 1980
    ..The methodology will include fluorometry, spectrophotometry, column, thin-layer and high pressure liquid chromatography, and extraction procedures.
  22. TWO TYPES OF MONOAMINE OXIDASE
    Jean Shih; Fiscal Year: 2007
    ..metabolites will be determined in MAO A/B double KO and wild type mice by[unreadable] high pressure liquid chromatography (HPLC)...
  23. MEIBOMIAN KERATOCONJUNCTIVITIS
    James McCulley; Fiscal Year: 1999
    ..b>High pressure liquid chromatography is also used in order to achieve effective initial characterizations...
  24. MECHANISMS OF TUMOR INITIATION BY BP AND DERIVATIVES
    Ercole Cavalieri; Fiscal Year: 1993
    ..9-FBP with rat liver microsomes and analyze the formation of the respective hydroxy derivatives by high pressure liquid chromatography. We will also investigate the ability of cysteine to act as a trapping agent for BP reactive ..
  25. ROLE OF T LYMPHOCYTE FACTORS IN HEMOPOIESIS 0
    Michael Prystowsky; Fiscal Year: 1992
    ..These studies will further our understanding of the role that T lymphocytes play in regulating hemopoiesis...
  26. ANESTHETICS ALTER VASCULAR CELL PHOSPHATIDYLINOSITOL
    Audrey Robinson White; Fiscal Year: 1990
    ..Techniques will include biochemical assays, radiolabeled assays, and high pressure liquid chromatography (HPLC) to assay for PI and components of the PI pathway and to obtain evidence of a structure-..
  27. IMMUNOBIOLOGY OF B CELL DIFFERENTIATION
    Kenneth Dorshkind; Fiscal Year: 1991
    ..weight of a factor secreted by S17 that induces Ig expression in pre-B cells will be determined by high pressure liquid chromatography. The relative contribution of the factor alone versus direct contact between developing B cells and ..
  28. ACTIVATION OF PROTEIN KINASE C IN VASCULAR SMOOTH MUSCLE
    Bradley Dixon; Fiscal Year: 1993
    ..In addition, gas chromatography, argentation thin layer chromatography, and high pressure liquid chromatography will be used to determine the molecular species of diacyglycerol released after exposure to these ..
  29. MECHANISM UNDERLYING DCYVD MEDIATED PREFERENTIAL STIMULA
    Kapil Bhalla; Fiscal Year: 1993
    ..we will carefully examine the accumulation of Ara- CTP; Ara-CTP to dCTP pool ratios (utilizing high pressure liquid chromatography methods); deamination of dCyd (dCMP) and Ara- C (Ara-CMP) by nucleoside and dCMP deaminase; ..
  30. Defining PcsB: Essential Murein Biosynthesis Protein in Pathogenic Pneumococci
    SKYE BARENDT; Fiscal Year: 2009
    ..PcsB by examining the extent of cross-linking in an in vitro atmosphere by means of reverse-phase high pressure liquid chromatography and mass spectrometry methods...
  31. STEROID HORMONES IN BREAST CANCER
    RICHARD HOCHBERG; Fiscal Year: 1980
    ..These studies will be performed by radiochemical techniques in conjunction with purification by high pressure liquid chromatography and identification by gas chromatography/mass spectroscopy, as well as other classical methods...
  32. MEMBRANE LIPIDS AND THEIR POTENTIAL ROLE IN LUPUS
    THOMAS SANTORO; Fiscal Year: 1992
    ..These abnormalities render macrophages proinflammatory. Using a number of analytical systems (high pressure liquid chromatography, thin layer chromatography, gas-liquid chromatography/mass spectrometry, enzyme and ..
  33. FIBROCARTILAGINOUS KNEE JOINT MENISCI: BIOLOGY & REPAIR
    Richard Webber; Fiscal Year: 1991
    ..Thus, insights gained from the proposed research can be expected to further our understanding of synovial joint physiology and thereby aid in design and selection of treatment modalities to be used by the clinican...
  34. CHALONE REGULATION OF HUMAN LYMPHOID NEOPLASMS
    David Ranney; Fiscal Year: 1980
    ..Final purification is currently being performed by high pressure liquid chromatography, using a C-18 reverse-phase column...
  35. SURGICAL STUDIES ON GALLBLADDER INFLAMMATION
    Stuart Myers; Fiscal Year: 1992
    ..Eicosanoids will be analyzed by radiochromatography and reversed phase high pressure liquid chromatography and RIA...
  36. CELLULAR CORRELATES OF PERINATAL NEUROENDOCRINOPATHIES
    PAUL RUDEEN; Fiscal Year: 1990
    ..b>High pressure liquid chromatography (HPLC) with electrochemical detection will be used for measuring hypothalamic catecholamine content ..
  37. The Role of Visual Stimuli in Emmetropization and Myopia
    Alan Busby; Fiscal Year: 2006
    ..Among these biochemical techniques are: protein assays, high pressure liquid chromatography, ion exchange chromatography, luciferase assay, DNA quantitation, cell culture techniques, and ..
  38. MECHANISM OF AMINO ACID NEUROTRANSMITTER RELEASE
    Tetsufumi Ueda; Fiscal Year: 1993
    ..to (a) purify the stimulatory (or obligatory) proteinaceous factor by classical chromatography and high pressure liquid chromatography; (b) characterize the glutamate release from permeabilized synaptosomes with respect to (i) ..
  39. LIQUID CHROMATOGRAPHY IN CAPILLARY COLUMNS
    James Jorgenson; Fiscal Year: 2000
    DESCRIPTION: (adapted from applicant's abstract) This research is to develop a new technology, Ultra High Pressure Liquid Chromatography (UHPLC), for the rapid separation and analysis of complex mixtures of biological origin...
  40. IN VITRO & IN VIVO MODEL OF MENISCAL WOUND REPAIR
    Richard Webber; Fiscal Year: 1990
    ..Thus, insights gained from the proposed research can be expected to futher our understanding of synovial joint physiology and thereby aid in the design and selection of treatment modalities to be used by the clinicians...
  41. PLASMACYTOMA RESISTANCE TO ALKYLATING AGENTS
    Carmelita Frondoza; Fiscal Year: 1993
    ..products in tumor cell homogenates after incubating with radiolabeled 4HC and glutathione by high pressure liquid chromatography and mass spectrometry. 3...
  42. ANTIAIDS AGENTS FROM MARINE ORGANISMS
    Mark Hamann; Fiscal Year: 2000
    ..activity against infectious targets will be isolated using preparative and semi-preparative high pressure liquid chromatography (HPLC), independently and interfaced with NMR and FTMS...
  43. INHIBITION OF CARCINOGENESIS BY SELENIUM
    Arthur Cohen; Fiscal Year: 1980
    ..Recently developed high pressure liquid chromatography techniques will be used to separate and quantitate metabolites of FAA excreted in the urine...
  44. CANCER-INDUCED COAGULATION--MONOCYTES/HYALURONIC ACID
    Maria McGee; Fiscal Year: 1992
    ..5) Polyclonal antibody production. 6) Immunoaffinity chromatography. 7) High pressure liquid chromatography (HPLC). Oligossacharides (HA5 to HA20) and larger molecular weight HA fragments (...
  45. THE GENETIC TOXICOLOGY OF TWO S-TRIAZINE HERBICIDES
    PETER MC GUIRE; Fiscal Year: 1980
    ..b>High pressure liquid chromatography will be used to isolate preparative amounts of mutagen(s), after we have determined which peak(s) ..
  46. REGULATION OF JUVENILE HORMONE SYNTHESIS
    Barbara Stay; Fiscal Year: 2003
    ....
  47. FETAL ALCOHOL NEUROENDOCRINOPATHIES
    PAUL RUDEEN; Fiscal Year: 1991
    ..b>High pressure liquid chromatography (HPLC) with electrochemical detection will be used for measuring catecholamine content and turnover...
  48. Elovl4 in Retinal Physiology and Pathophysiology
    Wojciech Kedzierski; Fiscal Year: 2009
    ..The lipids will be analyzed using thin layer chromatography and high pressure liquid chromatography combined with mass spectrometry...
  49. DNA TOPOISOMERASE I TARGET INTERACTIONS OF CAMPTOTHECINS
    Danzhou Yang; Fiscal Year: 2004
    ..including high field nuclear magnetic resonance spectroscopy (NMR), computer molecular modeling, high pressure liquid chromatography, high sensitivity differential scanning c a l o rimetry and isothermal titration calorimetry, ..
  50. MECHANISMS OF ACTIVATION OF POLYCYCLIC HYDROCARBONS
    David Phillips; Fiscal Year: 1991
    ..b>High pressure liquid chromatography will assist in the metabolic studies and will be incorporated into the 32p-postlabelling assay...
  51. PATHOGENESIS OF MICROANGIOPATHY IN DIABETES
    JOHN SOELDNER; Fiscal Year: 1980
    ..A high pressure liquid chromatography system for the accurate and reproducible determination of hemoglobin AIc has been perfected...
  52. Striatal Acetylcholine and Behavioral Flexibility
    MICHAEL RAGOZZINO; Fiscal Year: 2005
    ..acquisition and reversal learning of a visual cue discrimination, using in vivo microdialysis with high pressure liquid chromatography. Recent timings in Parkinson's disease patients suggest that anti-cholinergic treatments lead to ..
  53. VOLUME REGULATION & MEMBRANE TRANSPORT IN ASTROCYTES
    HAROLD KIMELBERG; Fiscal Year: 1992
    ..high voltage electron microscopy to study as [3H] taurine, [3H] glutamate and [3H] D-aspartate of high pressure liquid chromatography to study the authentic processes will be done...
  54. ARGININE VASOTOCIN IN THE OVINE FETUS AND NEWBORN
    M Ervin; Fiscal Year: 1991
    ..Previously, we have employed specific antisera for AVT, AVP and OXT combined with high pressure liquid chromatography to identify AVT, AVP and OXT in plasma of fetal lambs and human newborns...
  55. STRUCTURE/FUNCTION OF HEME REGULATED ELF-2ALPHA KINASE
    Jane Jane Chen; Fiscal Year: 2001
    ..are recombinant DNA techniques, polymerase chain reaction, synthesis of oligodeoxynucleotides, high pressure liquid chromatography, amino acid microsequencing, in vitro transcription and translation, protein kinase assays, gel ..
  56. CHRONIC MYELOGENOUS LEUKEMIA--THERAPEUTIC APPROACH
    Neil Gibson; Fiscal Year: 1992
    ..DNA base adducts will be analyzed by high pressure liquid chromatography and identified by mass spectroscopy...
  57. REPAIR SYNTHESIS OF DNA IN PRECANCEROUS RAT LIVER
    Jay Goodman; Fiscal Year: 1980
    ..liver will be enzymatically digested to nucleosides which will be separated, by employing high pressure liquid chromatography, to discern specific reaction products of the carcinogen with DNA bases...
  58. STRUCTURE ELUCIDATION OF HUMAN TRANSFER FACTOR
    G Daves; Fiscal Year: 1980
    ..b) TF can be purified by gel filtration, isoelectric focusing (IEF), and reverse phase, high pressure liquid chromatography (HPLC), (c) the purified TF is not inactivated by alkaline phosphatase although its fractionation ..
  59. NOVEL TRICLOSAN RESISTANCE MECHANISM IN BACTERIA
    MAURA MEADE; Fiscal Year: 2000
    ..b>High pressure liquid chromatography will be utilized to determine if the triclosan concentration decreases over time in these cultures...
  60. REGULATION OF EPIDERMAL STEROL METABOLISM IN AGING
    D Pehlke; Fiscal Year: 1980
    ..Cholesterol and 7-dehydrocholesterol will be measured in aging human epidermis by using high pressure liquid chromatography, TLC, and gas liquid chromatography...
  61. Identification of Short-Lived Radicals in Tobacco Smoke
    Sarah Green; Fiscal Year: 2007
    ..case the radicals are trapped to form stable, fluorescent diamagnetic adducts which are analyzed by high pressure liquid chromatography (HPLC). Coupling to a mass sectrometer (LC-MS) will allow the radical species to be identified...
  62. QUANTITATIVE DETERMINATION OF CATECHOLAMINES
    James Girard; Fiscal Year: 1980
    ..Recently High Pressure Liquid Chromatography with a Amperometric electrochemical detector has become widely used...