agarose chromatography

Summary

Summary: A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million.

Top Publications

  1. ncbi NAP-2 is part of multi-protein complexes in HeLa cells
    Pedro Rodriguez
    McGill Cancer Center, McGill University, Montreal, Quebec, Canada H3G 1Y6
    J Cell Biochem 93:398-408. 2004
  2. ncbi Isolation of transcription factor complexes by in vivo biotinylation tagging and direct binding to streptavidin beads
    Patrick Rodriguez
    Department of Cell Biology, Erasmus Medical Center, Rotterdam, The Netherlands
    Methods Mol Biol 338:305-23. 2006
  3. pmc The high concentration of Arg213-->Gly extracellular superoxide dismutase (EC-SOD) in plasma is caused by a reduction of both heparin and collagen affinities
    Steen V Petersen
    Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10C, DK 8000 Aarhus C, Denmark
    Biochem J 385:427-32. 2005
  4. ncbi Purification of elongation factors EF-Tu and EF-G from Escherichia coli by covalent chromatography on thiol-sepharose
    T D Caldas
    Biochimie Génétique, Institut Jacques Monod, Universite Paris 7, 2 place Jussieu, Paris Cedex 05, 75251, France
    Protein Expr Purif 14:65-70. 1998
  5. ncbi Cloning and characterization of the catabolite control protein A gene from Bacillus stearothermophilus No. 236
    Il Dong Choi
    Graduate School of Life Science and Biotechnology, Korea University, Seoul 136 701, Korea
    Biosci Biotechnol Biochem 68:1414-23. 2004
  6. ncbi Cloning and expression in Escherichia coli of the D-aspartate oxidase gene from the yeast Cryptococcus humicola and characterization of the recombinant enzyme
    Shouji Takahashi
    Department of Environmental Systems Engineering, Nagaoka University of Technology, Nagaoka, Niigata 940 2188
    J Biochem 135:533-40. 2004
  7. ncbi General strategies
    Shawn Doonan
    School of Biosciences, University of East London, UK
    Methods Mol Biol 244:1-13. 2004
  8. ncbi Enhanced expression of a recombinant malaria candidate vaccine in Escherichia coli by codon optimization
    Zhiyong Zhou
    Division of Parasitic Diseases, Centers for Disease Control and Prevention, 4770 Buford Highway, Chamblee, GA 30341, USA
    Protein Expr Purif 34:87-94. 2004
  9. ncbi Rapid, high-throughput purification of HIV-1 integrase using microtiter plate technology
    Tan Wang
    Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003, USA
    Protein Expr Purif 33:232-7. 2004
  10. ncbi Bacterial expression and purification of the Arabidopsis NADPH-cytochrome P450 reductase ATR2
    A K Hull
    Department of Biology, Boston University, 5 Cummington Street, Boston, Massachusetts, 02215, USA
    Protein Expr Purif 18:310-5. 2000

Research Grants

  1. X-LINKED OCULAR ALBINISM & RETINITIS PIGMENTOSA
    Anand Swaroop; Fiscal Year: 1993
  2. ROLE OF PHOSPHOLIPIDS IN MINERALIZATION OF OSTEOID
    David Howell; Fiscal Year: 1992
  3. PHOSPHOLIPIDS AND MINERALIZATION OF OSTEOID
    David Howell; Fiscal Year: 1999
  4. LEUKOCYTE INTERACTIONS WITH EXTRACELLULAR MATRIX
    ARTHUR MERCURIO; Fiscal Year: 1999
  5. PCP RECEPTOR CLASSES: STRUCTURE & THERAPEUTIC ASPECTS
    MORDECHAI SOKOLOVSKY; Fiscal Year: 1990
  6. ENZYMATIC N-METHYLATION OF CALMODULIN
    FRANK SIEGEL; Fiscal Year: 1993
  7. BIOCHEMISTRY OF NORMAL AND LEUKEMIC LYMPHOCYTES
    CREED ABELL; Fiscal Year: 1980
  8. QUANTITATIVE MODEL OF EGF-CELL INTERACTIONS
    H Wiley; Fiscal Year: 1992
  9. Tumor-reactive IgG in the diagnosis of ovarian cancer
    Douglas Taylor; Fiscal Year: 2005
  10. T Cell Memory to Respiratory Viral Infections
    Shahram Salek Ardakani; Fiscal Year: 2008

Detail Information

Publications232 found, 100 shown here

  1. ncbi NAP-2 is part of multi-protein complexes in HeLa cells
    Pedro Rodriguez
    McGill Cancer Center, McGill University, Montreal, Quebec, Canada H3G 1Y6
    J Cell Biochem 93:398-408. 2004
    ..This fraction, although able to supercoil relaxed DNA, did so to a lesser extent than the Q-Sepharose fraction. The data suggest that NAP-2 is in complex(es) with other proteins, which are distinct from histones...
  2. ncbi Isolation of transcription factor complexes by in vivo biotinylation tagging and direct binding to streptavidin beads
    Patrick Rodriguez
    Department of Cell Biology, Erasmus Medical Center, Rotterdam, The Netherlands
    Methods Mol Biol 338:305-23. 2006
    ..Thus, BirA-mediated tagging is an efficient approach for the direct capture and characterization of transcription factor complexes...
  3. pmc The high concentration of Arg213-->Gly extracellular superoxide dismutase (EC-SOD) in plasma is caused by a reduction of both heparin and collagen affinities
    Steen V Petersen
    Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10C, DK 8000 Aarhus C, Denmark
    Biochem J 385:427-32. 2005
    ..We conclude that the increased concentration of EC-SOD in the plasma of R213G carriers is caused by a reduction in both heparin and collagen affinities...
  4. ncbi Purification of elongation factors EF-Tu and EF-G from Escherichia coli by covalent chromatography on thiol-sepharose
    T D Caldas
    Biochimie Génétique, Institut Jacques Monod, Universite Paris 7, 2 place Jussieu, Paris Cedex 05, 75251, France
    Protein Expr Purif 14:65-70. 1998
    ..The specific reactivities of the elongation factors with thiol-Sepharose allow their efficient purification and suggest that they possess hitherto undiscovered properties connected with their reactive thiols...
  5. ncbi Cloning and characterization of the catabolite control protein A gene from Bacillus stearothermophilus No. 236
    Il Dong Choi
    Graduate School of Life Science and Biotechnology, Korea University, Seoul 136 701, Korea
    Biosci Biotechnol Biochem 68:1414-23. 2004
    ..236 bound specifically to the xynA creB (catabolite responsive element B) sequence. Taken together, these results strongly suggest that the CcpA protein participates in CCR of B. stearothermophilus No. 236 xynA gene...
  6. ncbi Cloning and expression in Escherichia coli of the D-aspartate oxidase gene from the yeast Cryptococcus humicola and characterization of the recombinant enzyme
    Shouji Takahashi
    Department of Environmental Systems Engineering, Nagaoka University of Technology, Nagaoka, Niigata 940 2188
    J Biochem 135:533-40. 2004
    ..ChDDO was expressed in an active form in E. coli to an approximately 5-fold greater extent than in yeast. The purified recombinant enzyme was identical to the native enzyme in physicochemical and catalytic properties...
  7. ncbi General strategies
    Shawn Doonan
    School of Biosciences, University of East London, UK
    Methods Mol Biol 244:1-13. 2004
  8. ncbi Enhanced expression of a recombinant malaria candidate vaccine in Escherichia coli by codon optimization
    Zhiyong Zhou
    Division of Parasitic Diseases, Centers for Disease Control and Prevention, 4770 Buford Highway, Chamblee, GA 30341, USA
    Protein Expr Purif 34:87-94. 2004
    ..Thus, codon optimization significantly increases the yield of P. falciparum candidate vaccines in the E. coli expression system...
  9. ncbi Rapid, high-throughput purification of HIV-1 integrase using microtiter plate technology
    Tan Wang
    Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003, USA
    Protein Expr Purif 33:232-7. 2004
    ..This high-throughput platform should be of general utility to those interested in defining the structure-function relationship of proteins and enzymes...
  10. ncbi Bacterial expression and purification of the Arabidopsis NADPH-cytochrome P450 reductase ATR2
    A K Hull
    Department of Biology, Boston University, 5 Cummington Street, Boston, Massachusetts, 02215, USA
    Protein Expr Purif 18:310-5. 2000
    ..4 micromol min(-1) mg(-1) and the K(m) for cytochrome c reduction was 15 +/- 2 microM. The purified NADPH-cytochrome P450 reductase was able to support function of CYP79B2...
  11. ncbi A method for the purification of Shiga-like toxin 1 subunit B using a commercially available galabiose-agarose resin
    Maria Teresa Tarrago-Trani
    Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061, USA
    Protein Expr Purif 38:170-6. 2004
    ..The resin was stable over 3 years and 500 cycles/year of usage. Hence, this method is a straightforward approach to the large-scale preparation of SLTB at a reasonable cost...
  12. ncbi Comparative evaluation of two purification methods of anti-CD19-c-myc-His6-Cys scFv
    Dipankar Das
    Department of Pharmacology, University of Alberta, Edmonton, AB, Canada T6G 2H7
    Protein Expr Purif 39:199-208. 2005
    ....
  13. ncbi Two kinds of chlorocatechol 1,2-dioxygenase from 2,4-dichlorophenoxyacetate-degrading Sphingomonas sp. strain TFD44
    Gang hua Lang
    National Institute for Agro Environmental Sciences, 3 1 3 Kan nondai, Tsukuba, Ibaraki 305 8604, Japan
    Biochem Biophys Res Commun 332:941-8. 2005
    ..Whereas phylogenetic analysis indicated that the CCDs of Sphingomonas constitute a distinctive group among Gram-negative bacteria, TfdC and TfdC2 of TFD44 have divergently evolved in terms of their substrate specificity...
  14. ncbi Osmo-expression and fast two-step purification of Escherichia coli translation termination factor RF-3
    K K Mortensen
    Department of Chemistry, Aarhus University, Denmark
    Eur J Biochem 234:732-6. 1995
    ..All assays showed that the purified RF-3 was highly active with a specific activity of approximately 2000 units/mg...
  15. ncbi Novel carbohydrate-binding activity of bovine liver beta-glucuronidase toward lactose/N-acetyllactosamine sequences
    Hiroko Matsushita-Oikawa
    Course of Advanced Biosciences, Graduate School of Humanities and Sciences, Tokyo, Japan
    Glycobiology 16:891-901. 2006
    ..The biological significance of the carbohydrate-specific interaction of beta-glucuronidase, which is different from the substrate recognition, is discussed...
  16. ncbi Production of recombinant Bartonella henselae 17-kDa protein for antibody-capture enzyme-linked immunosorbent assay
    Chien Chang Loa
    Department of Research and Development, Medical Diagnostic Laboratories, L L C, Hamilton, NJ 08690, USA
    Diagn Microbiol Infect Dis 55:1-7. 2006
    ..823. These results indicate that the expressed 17-kDa protein is a suitable source of antigen for development of an antibody-capture ELISA for the detection of antibodies to B. henselae...
  17. ncbi Identification and isolation from breast milk of ceruloplasmin-lactoferrin complex
    A V Sokolov
    Institute for Experimental Medicine, Russian Academy of Medical Sciences, 197376 St Petersburg, Russia
    Biochemistry (Mosc) 71:160-6. 2006
    ..The resulting complex Cp-Lf (1 : 1) was separated into components by chromatography on heparin-Sepharose. Limited tryptic hydrolysis of Cp obtained from BM and from blood plasma revealed identical proteolytic fragments...
  18. ncbi Role of Hirudin-like factor Va heavy chain sequences in prothrombinase function
    Raffaella Toso
    Department of Pediatrics, Division of Hematology, The Children s Hospital of Philadelphia and University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 281:8773-9. 2006
    ....
  19. ncbi Formation of albumin dimers induced by exposure to peroxides in human plasma: a possible biomarker for oxidative stress
    Yuki Ogasawara
    Department of Environmental Biology, Meiji Pharmaceutical University, 2 522 1 Noshio, Kiyose, Tokyo 204 8588, Japan
    Biochem Biophys Res Commun 340:353-8. 2006
    ..The present results indicate that albumin dimers are oxidative products derived from peroxides, and that their presence in plasma might be a marker of oxidative stress as secondary metabolites of peroxidation...
  20. ncbi [Purification and properties of recombinant Erwinia carotovora L-asparaginase expressed in E.coli cells]
    A A Borisova
    Biomed Khim 49:502-7. 2003
    ..Physical-chemical and structural properties of recombinant Erwinia carotovora L-asparaginase are similar to the enzymes from the wild strains Erwinia carotovora and recombinant L-asparaginase Erwinia chrysanthemi...
  21. ncbi Cloning, sequencing, expression, and single-step purification of the adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (CobU) from Salmonella typhimurium ATCC 19585
    Fang Ciao Hsu
    Institute of Biotechnology and Department of Chemical Engineering, National Taipei University of Technology, Taiwan
    Protein Expr Purif 42:178-81. 2005
    ..typhimurium ATCC 19585 and, without introducing a purification tag sequence to the N- or C-terminus of the recombinant enzyme, a new single-step purification method based on hydrophobic interaction chromatography...
  22. ncbi Specificity determinants in the interaction of apolipoprotein(a) kringles with tetranectin and LDL
    Nigel R Caterer
    Laboratory of Gene Expression, Department of Molecular and Structural Biology, Aarhus, DK 8000 Aarhus C, Denmark
    Biol Chem 383:1743-50. 2002
    ..Ligand binding analysis of these derivatives showed that Y62, and to a minor extent W32 and E56, of KIV(7) are important for LDL binding to KIV(7), whereas R32 and D56 of KIV(10) are required for tetranectin binding of KIV(10)...
  23. ncbi Characterization and tRNA recognition of mammalian mitochondrial seryl-tRNA synthetase
    T Yokogawa
    Department of Biomolecular Science, Faculty of Engineering, Gifu University, 1 1 Yanagito, Gifu 501 1193, Japan
    J Biol Chem 275:19913-20. 2000
    ..In addition, the human mt SerRS gene was found to be located on chromosome 19q13.1, to which the autosomal deafness locus DFNA4 is mapped...
  24. ncbi Cloning, expression, and purification of the His(6)-tagged thermostable beta-galactosidase from Pyrococcus woesei in Escherichia coli and some properties of the isolated enzyme
    S Daabrowski
    Department of Microbiology, Technical University of Gdansk, ul Narutowicza 11 12, Gdansk, 80 952, Poland
    Protein Expr Purif 19:107-12. 2000
    ..The enzyme was characterized and displayed high activity and thermostability. This bacterial expression system appears to be a good method for production of the thermostable beta-galactosidase...
  25. pmc Expression, purification and characterization of recombinant human choline acetyltransferase: phosphorylation of the enzyme regulates catalytic activity
    T Dobransky
    Department of Physiology, Medical Sciences Building, University of Western Ontario, London, Ontario, Canada N6A 5C1
    Biochem J 349:141-51. 2000
    ..These studies are of functional significance as they report for the first time that phosphorylation enhances choline acetyltransferase catalytic activity...
  26. ncbi Purification and characterization of the maize amyloplast stromal 112-kDa starch phosphorylase
    H H Mu
    Department of Food Science, Cook College, New Jersey Agricultural Experiment Station, Rutgers University, New Brunswick 08901, USA
    Arch Biochem Biophys 388:155-64. 2001
    ..The specificity constants (Vmax/Km) of the enzyme measured in either the synthetic or the phosphorolytic directions increased with increasing chain length...
  27. ncbi Purification and kinetic properties of betaine-homocysteine methyltransferase from Aphanothece halophytica
    R Waditee
    Laboratory of Biochemical Products, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
    Curr Microbiol 43:107-11. 2001
    ..5 mM betaine aldehyde completely abolished the enzyme activity. NaCl at 200 mM or higher also completely inactivated the enzyme...
  28. ncbi Purification and partial characterisation of alpha(2)-antiplasmin and plasmin(ogen) from ostrich plasma
    A R Thomas
    Department of Biochemistry and Microbiology, University of Port Elizabeth, P.O. Box 1600, 6000, Port Elizabeth, South Africa
    Comp Biochem Physiol B Biochem Mol Biol 129:809-20. 2001
    ..0 and 40 degrees C, respectively...
  29. ncbi Rapid two-step purification of a recombinant mouse Fab fragment expressed in Escherichia coli
    H Wlad
    Center for Surface Biotechnology, Uppsala Biomedical Center, Uppsala, SE-751 23, Sweden
    Protein Expr Purif 22:325-9. 2001
    ..The purified Fab57P fragment showed one band in SDS-PAGE. The overall purification yield was 35%...
  30. ncbi Purification and characterization of N-acetylglucosaminyl sulfotransferase from chick corneas
    Y Yamamoto
    Section of Radiochemistry, Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tempaku-ku, Nagoya 468-8503, Japan
    Arch Biochem Biophys 392:87-92. 2001
    ..It appears that this enzyme competes with beta-galactosyltransferase in binding to the nonreducing GlcNAc residue on KS synthesis; this suggests that the sulfation of the GlcNAc residue is coupled with the elongation of the KS chain...
  31. ncbi Biochemical and functional analysis of a conserved IGF-binding protein isolated from rainbow trout (Oncorhynchus mykiss) hepatoma cells
    J R Bauchat
    Department of Biology, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Endocrinol 170:619-28. 2001
    ..These results indicate that the IGFBP molecule is structurally and functionally conserved in evolutionarily ancient vertebrate species such as bony fish...
  32. ncbi Conformational changes in thrombin when complexed by serpins
    J C Fredenburgh
    Hamilton Civic Hospitals Research Centre and Department of Medicine, McMaster University, Hamilton, Ontario L8V 1C3, Canada
    J Biol Chem 276:44828-34. 2001
    ..The disorganization of exosite 1 that occurs when thrombin is complexed by serpins is consistent with results of protease sensitivity studies and crystallographic analysis of a homologous enzyme-serpin complex...
  33. ncbi Characterization of nucleoside diphosphate kinase from moderately halophilic eubacteria
    Y Yonezawa
    Laboratory of Applied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University, Korimoto, Japan
    Biosci Biotechnol Biochem 65:2343-6. 2001
    ..This protein showed anomalous mobility on SDS-PAGE which is typical of a halophilic protein. It was stable and active over a wide range of salt concentrations, from 0 to 4.0 M NaCl...
  34. ncbi A new method for purification of human plasma retinol-binding protein and transthyretin
    Pullakhandam Raghu
    Department of Biophysics, National Institute of Nutrition, Indian Council of Medical Research, Jamai Osmania, Hyderabad 500 007, India
    Biotechnol Appl Biochem 38:19-24. 2003
    ..Furthermore, the purified RBP and TTR were found to be functional from mutual binding monitored by fluorescence quenching...
  35. ncbi Novel lectin-like proteins on the surface of human monocytic leukemia cell line THP-1 cells that recognize oxidized cells
    S Eda
    School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan
    Arch Biochem Biophys 385:186-93. 2001
    ....
  36. ncbi Use of ceramic monoliths as stationary phase in affinity chromatography
    Eva Martin del Valle
    Department of Chemical and Materials Engineering, University of Alabama in Huntsville, Huntsville, Alabama 35899, USA
    Biotechnol Prog 19:921-7. 2003
    ..Adsorption and desorption rates are large and production of enzyme per unit monolith volume justifies further exploring this separation process for large throughput operation...
  37. ncbi Parotid salivary basic proline-rich proteins inhibit HIV-I infectivity
    M R Robinovitch
    Department of Periodontics and Oral Biology, School of Dentistry, University of Washington, Seattle, WA 98195, USA
    Oral Dis 7:86-93. 2001
    ....
  38. ncbi Purification and biochemical characterization of a basic superantigen (SPEX/SMEZ3) from Streptococcus pyogenes
    D Gerlach
    Institute of Medical Microbiology, Friedrich Schiller University Jena, Semmelweisstr, Jena, Germany
    FEMS Microbiol Lett 188:153-63. 2000
    ..The purified SPEX stimulated human T-lymphocytes with Vbeta8 specificity at extremely low concentrations (lower than 100 pg ml(-1))...
  39. ncbi Purification, characterization and functional cloning of inositol oxygenase from Cryptococcus
    Ulrike Kanter
    University of Frankfurt, Biocentre, Plant Molecular Biology, Marie Curie Strasse 9, 60439 Frankfurt, Germany
    Yeast 20:1317-29. 2003
    ..5 and a temperature optimum of 30 degrees C. Myo-inositol is the only accepted substrate with a Km of ca. 5 mM. The enzyme contains a Fe-centre but the enzyme activity is resistant to KCN...
  40. ncbi Expression, purification and spectroscopic studies of full-length Kir3.1 channel C-terminus
    Robert N Leach
    School of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK
    Biochim Biophys Acta 1652:83-90. 2003
    ..These observations demonstrate that full-length Kir3.1 C-terminus can be purified in a stable conformation capable of binding proteins known to activate Kir3 channels and may contain elements involved in channel assembly...
  41. ncbi Studies on purification of methamidophos monoclonal antibodies and comparative immunoactivity of purified antibodies
    Su Qing Zhao
    Zhongshan Medical School, Sun Yat Sen Zhongshan University, Guangzhou 510089, China
    Biomed Environ Sci 16:119-25. 2003
    ..To purify Methamidophos (Met) monoclonal antibodies with two methods and compare immune activity of purified antibodies...
  42. ncbi Characteristics of the interaction between Hsc70 and the transferrin receptor in exosomes released during reticulocyte maturation
    C Geminard
    UMR CNRS 5539, Universite Montpellier II, cc107, 34095 Montpellier, France
    J Biol Chem 276:9910-6. 2001
    ..Moreover, we used immunosuppressive agents that interact with Hsc70, thus decreasing Hsc70 binding to TfR in our in vitro binding assay and enabling us to assess the role of this interaction in vivo during reticulocyte maturation...
  43. ncbi Dynamic O-glycosylation of nuclear and cytosolic proteins: cloning and characterization of a neutral, cytosolic beta-N-acetylglucosaminidase from human brain
    Y Gao
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2185, USA
    J Biol Chem 276:9838-45. 2001
    ....
  44. ncbi TbMP57 is a 3' terminal uridylyl transferase (TUTase) of the Trypanosoma brucei editosome
    Nancy Lewis Ernst
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Mol Cell 11:1525-36. 2003
    ..Recombinant TbMP57 specifically interacts with the editosome protein TbMP81, and this interaction enhances the TUTase activity. These results suggest that TbMP57 catalyzes U addition to pre-mRNA during editing...
  45. ncbi Overexpression and purification of the membrane-bound cytochrome P450 2B4
    A S Saribas
    Department of Anesthesiology, University of Michigan, Ann Arbor, Michigan 48108, USA
    Protein Expr Purif 21:303-9. 2001
    ..The yield of cyt P450 from the improved purification procedure is twice that from the original procedure and the purity of the recovered protein typically has a specific content of 17 nmol cyt P450/mg of protein...
  46. ncbi Purification of eukaryotic MutL homologs from Saccharomyces cerevisiae using self-affinity technology
    M C Hall
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Protein Expr Purif 21:333-42. 2001
    ..The general approach is a valid alternative for simple, rapid purification of recombinant proteins in yeast when expression in bacteria is unsuitable...
  47. ncbi Flavin reductase coupling with two monooxygenases involved in dibenzothiophene desulfurization: purification and characterization from a non-desulfurizing bacterium, Paenibacillus polymyxa A-1
    T Ohshiro
    Department of Biotechnology, Tottori University, Tottori 680 8552, Japan
    Appl Microbiol Biotechnol 59:649-57. 2002
    ..The result of the coupling reaction suggested that, in the microbial DBT desulfurization, flavin reductase from the non-DBT-desulfurizing bacterium was superior to that from the DBT-desulfurizing bacterium...
  48. pmc Isolation, by partial pepsin digestion, of the three collagen-like regions present in subcomponent Clq of the first component of human complement
    K B Reid
    Biochem J 155:5-17. 1976
    ..50% higher than expected from the amino acid sequence data. This is probably due to the high collagen-like sequence content of these fragments...
  49. ncbi Rapid isolation and biochemical characterization of rat C1 and C1q
    M G Wing
    Molecular Immunopathology Unit, MRC Centre, Cambridge, U K
    Mol Immunol 30:433-40. 1993
    ..In addition to the major A:B and C:C dimer bands, rat, unlike human C1q, contained minor dimer species. These may reflect heterogeneity in glycosylation and or lysine and proline hydroxylation...
  50. ncbi Purification of rabbit and human serum paraoxonase
    C E Furlong
    Division of Medical Genetics, School of Medicine, University of Washington, Seattle 98195
    Biochemistry 30:10133-40. 1991
    Rabbit serum paraoxonase/arylesterase has been purified to homogeneity by Cibacron Blue-agarose chromatography, gel filtration, DEAE-Trisacryl M chromatography, and preparative SDS gel electrophoresis. Renaturation (Copeland et al...
  51. ncbi [Abundant protein' depletion in search of biomarkers]
    Alex J Rai
    Department of Clinical Laboratories, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, Box 88, New York, NY 10021, USA
    Med Sci (Paris) 23:5-8. 2007
    ..Finally, considerations for the translation of biomarkers, identified in such a manner, to a clinically useful assay, are discussed...
  52. ncbi Purification of sequence-specific DNA-binding proteins by affinity chromatography
    L A Kerrigan
    University of California San Diego, La Jolla, California, USA
    Curr Protoc Mol Biol . 2001
    ..Parameters essential to the success of an affinity chromatography experiment are discussed in detail in the Commentary...
  53. ncbi Purification and properties of a novel beta-glucosidase, hydrolyzing ginsenoside Rb1 to CK, from Paecilomyces Bainier
    Qin Yan
    School of Pharmacy and Institutes of Biomedical Sciences, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China
    J Microbiol Biotechnol 18:1081-9. 2008
    ..By comparing the five identified peptide sequences with the NCBI database, this purified beta-glucosidase proves to be a new protein that has not been reported before...
  54. ncbi Chromatography of human immunoglobulin G on immobilized drimarene rubine R/K-5BL. Study of mild, efficient elution procedures
    S Cochet
    INSERM U 76, Paris, France
    J Chromatogr A 663:175-86. 1994
    ..A procedure was also devised for separating IgG2 from other IgG subclasses using chromatography on immobilized Rubine R/K-5BL and column development with an AMP gradient...
  55. ncbi The study of Escherichia coli proteases. Intracellular serine protease of E. coli-an analogue of bacillus proteases
    A Y Strongin
    J Gen Microbiol 110:443-51. 1979
    ..coli protease was similar to that of the Bacillus licheniformis enzyme. These data indicate a relationship between proteolytic enzymes of evolutionary distant Gram-negative Enterobacteriaceae and Gram-positive spore-forming Bacillus...
  56. ncbi Purification and characterization of three toxins and two agglutinins from Abrus precatorius seed by using lactamyl-Sepharose affinity chromatography
    R Hegde
    Department of Biochemistry, Indian Institute of Science, Bangalore
    Anal Biochem 194:101-9. 1991
    ....
  57. ncbi Characterization of a tungsten-substituted nitrogenase isolated from Rhodobacter capsulatus
    Stefan Siemann
    Department of Chemistry, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada
    Biochemistry 42:3846-57. 2003
    ....
  58. pmc Identification of the human mitochondrial linoleoyl-coenzyme A monolysocardiolipin acyltransferase (MLCL AT-1)
    William A Taylor
    Department of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, Manitoba R3E 0T6, Canada
    J Biol Chem 284:30360-71. 2009
    ..The results identify MLCL AT-1 as a human mitochondrial monolysocardiolipin acyltransferase involved in the remodeling of cardiolipin...
  59. ncbi O-acetylation and de-O-acetylation of sialic acids. Purification, characterization, and properties of a glycosylated rat liver esterase specific for 9-O-acetylated sialic acids
    H H Higa
    Department of Medicine, San Diego Veterans Medical Center, California
    J Biol Chem 264:19435-42. 1989
    ..apparent homogeneity by multiple steps, including ConA-Sepharose affinity chromatography and Procion Red-agarose chromatography (yield, 13%; fold purification, approximately 3000). The homogeneous enzyme is a 61...
  60. ncbi Beta-glucoside kinase (BglK) from Klebsiella pneumoniae. Purification, properties, and preparative synthesis of 6-phospho-beta-D-glucosides
    John Thompson
    Microbial Biochemistry and Genetics Unit, Oral Infection and Immunity Branch, NIDCR National Institutes of Health, 30 Convent Drive, MSC 4350, Bethesda, MD 20892, USA
    J Biol Chem 277:34310-21. 2002
    ..Preparation of (His6)BglK by nickel-nitrilotriacetic acid-agarose chromatography provided high purity enzyme in quantity sufficient for the preparative synthesis (200-500 mg) of ten 6-..
  61. ncbi Effects of SH-modifying reagents on the rat hepatic Ah receptor: inhibition of ligand binding and transformation, and disruption of the ligand-receptor complex
    E C Henry
    Department of Biophysics, University of Rochester Medical Center, NY 14642
    Biochim Biophys Acta 964:361-76. 1988
    ..Our results are in striking contrast to the effects of these reagents on steroid receptors, whose bound steroid hormone ligand is rapidly and reversibly displaced by lower concentrations of reagent.(ABSTRACT TRUNCATED AT 400 WORDS)..
  62. ncbi [Investigation of hydrophobic characteristics of biofilm producer and non-producer Staphylococcus aureus clinical isolates]
    Selma Ay
    Inonu Universitesi Tip Fakultesi, Tibbi Mikrobiyoloji Anabilim Dali, Malatya
    Mikrobiyol Bul 44:221-30. 2010
    ..aureus strains were more hydrophobic than non-slime producing strains. Further research is required in order to characterise the eluted fractions and to evaluate their pathogenic capacities...
  63. ncbi Purification and cloning of a novel serine protease, RNK-Met-1, from the granules of a rat natural killer cell leukemia
    M J Smyth
    Cellular Cytotoxicity Laboratory, Austin Research Institute, Austin Hospital, Heidelberg, Victoria, Australia
    J Biol Chem 267:24418-25. 1992
    ..These data indicate that RNK-Met-1 is a serine protease with unique activity that is expressed in the granules of large granular lymphocytes...
  64. ncbi Purification, characterization, and drug susceptibility of tubulin from Leishmania
    K A Werbovetz
    Department of Parasitology, Walter Reed Army Medical Institute of Research, Washington, DC 20307, USA
    Mol Biochem Parasitol 98:53-65. 1999
    ....
  65. ncbi The Arg-4 mutant factor IX Strasbourg 2 shows a delayed activation by factor XIa
    C de la Salle
    INSERM U 311, Centre Regional de Transfusion Sanguine, Strasbourg, France
    Nouv Rev Fr Hematol 35:473-80. 1993
    ..In conclusion, factor IX Strasbourg 2 circulates with the attached propeptide and shows reduced gamma-carboxylation and delayed activation by factor XIa but a normal capacity to activate factor X after total cleavage by factor XIa...
  66. ncbi Cloning, expression, and purification of glyoxysomal 3-oxoacyl-CoA thiolase from sunflower cotyledons
    Anke C Schiedel
    Cell Biology, MSB, New York University Medical Center, New York, NY 10016, USA
    Protein Expr Purif 33:25-33. 2004
    ..in a bacterial system in an active form and purified to apparent homogeneity in a single step using Ni-NTA agarose chromatography. The enzyme was purified 53.4-fold and had a specific activity of 235 nkat/mg protein...
  67. ncbi Site-directed mutagenesis of the m2 muscarinic acetylcholine receptor. Analysis of the role of N-glycosylation in receptor expression and function
    C J van Koppen
    Department of Pharmacology, University of Washington, Seattle 98195
    J Biol Chem 265:20887-92. 1990
    ..Furthermore, N-glycosylation of the m2 mAChR is not required for functional coupling of the m2 mAChR to inhibition of adenylate cyclase...
  68. pmc Purification of prenylated proteins by affinity chromatography on cyclodextrin-modified agarose
    Jinhwa A Chung
    Department of Chemistry, University of Minnesota, Minneapolis, 55455, USA
    Anal Biochem 386:1-8. 2009
    ..We propose that this method could be generally useful to the community of researchers studying protein prenylation...
  69. ncbi The functional characteristics of a human apolipoprotein E variant (cysteine at residue 142) may explain its association with dominant expression of type III hyperlipoproteinemia
    Y Horie
    Gladstone Foundation Laboratories for Cardiovascular Disease, University of California, San Francisco 94140 0608
    J Biol Chem 267:1962-8. 1992
    ..The unique combination of functional properties of the Arg112, Cys142 variant provides a possible explanation for its association with dominant expression of type III hyperlipoproteinemia...
  70. ncbi Purification and properties of alpha-galactosidase from white-rot fungus Pleurotus florida
    - Ramalingam
    Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641 006, Tamil Nadu, India
    Indian J Biochem Biophys 44:76-81. 2007
    ..alpha-Galactosidase immobilized in agar was more effective for the degradation of raffinose than in the sodium alginate. TLC results indicated its potential for the removal of raffinose and stachyose in soymilk...
  71. ncbi Transferrin toxin but not transferrin receptor immunotoxin is influenced by free transferrin and iron saturation
    P H Gosselaar
    University Medical Centre Utrecht, Eijkman Winkler Institute G04 614, Heidelberglaan 100, PO Box 85500, 3508GA Utrecht, The Netherlands
    Eur J Clin Invest 32:61-9. 2002
    ..Cytotoxic agents can be targeted successfully to cancer cells. The efficacy of such novel and potent anticancer strategies may be influenced by variables of iron metabolism...
  72. pmc Use of recombinant flagellin protein as a tracer antigen in a fluorescence polarization assay for diagnosis of leptospirosis
    N I Bughio
    Canadian Food Inspection Agency, Animal Diseases Research Institute, Nepean, Ontario, Canada K2H 8P9
    Clin Diagn Lab Immunol 6:599-605. 1999
    ..The recombinant protein FlaB was purified to homogeneity by a combination of nickel-nitriloacetic acid agarose chromatography, electrophoresis, and electroelution. Purified FlaB was labeled with fluorescein isothiocyanate (FITC)...
  73. pmc Molecular cloning, expression, and partial characterization of a second human tissue-factor-pathway inhibitor
    C A Sprecher
    ZymoGenetics, Inc, Seattle, WA 98105
    Proc Natl Acad Sci U S A 91:3353-7. 1994
    ..baby hamster kidney cells and purified from the serum-free conditioned medium by a combination of heparin-agarose chromatography, Mono Q FPLC, Mono S FPLC, and Superose 12 FPLC...
  74. pmc Novel application of S-nitrosoglutathione-Sepharose to identify proteins that are potential targets for S-nitrosoglutathione-induced mixed-disulphide formation
    P Klatt
    Department of Estructura y Función de Proteínas, Centro de Investigaciones Biologicas, Instituto Reina Sofía de Investigaciones Nefrológicas, Consejo Superior de Investigaciones Cientificas, Velazquez 144, E 28006 Madrid, Spain
    Biochem J 349:567-78. 2000
    ....
  75. ncbi A role for a Hevea latex lectin-like protein in mediating rubber particle aggregation and latex coagulation
    Rapepun Wititsuwannakul
    Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand
    Phytochemistry 69:339-47. 2008
    ..This paper, for the first time, provides clear and unequivocal evidence for either a key biological role or physiological function of an endogenous latex lectin-like protein in the sequential process of latex coagulation...
  76. ncbi Large-scale recovery of C-phycocyanin from Spirulina platensis using expanded bed adsorption chromatography
    Jian Feng Niu
    Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
    J Chromatogr B Analyt Technol Biomed Life Sci 850:267-76. 2007
    ..SDS-PAGE gave two bands corresponding to 21 and 18 kDa. In-gel digestion and LC-ESI-MS showed that the protein is C-phycocyanin...
  77. ncbi Purification of granulocyte chemotactic peptide/interleukin-8 reveals N-terminal sequence heterogeneity similar to that of beta-thromboglobulin
    J Van Damme
    Rega Institute for Medical Research, University of Leuven, Belgium
    Eur J Biochem 181:337-44. 1989
    ..GCP/IL-8 was found to be chemotactic for granulocytes with a specific activity of 10(5) units/mg, whereas none of the beta-thromboglobulin species possessed detectable chemotactic activity...
  78. ncbi Type I procollagen C-proteinase from mouse fibroblasts. Purification and demonstration of a 55-kDa enhancer glycoprotein
    E Kessler
    Maurice and Gabriela Goldschleger Eye Research Institute, Tel Aviv University Faculty of Medicine, Sheba Medical Center, Tel Hashomer, Israel
    Eur J Biochem 186:115-21. 1989
    ..Also suggested from this study is that lysyl-Sepharose chromatography is a highly beneficial purification step which may find use in the purification of the C-proteinase from other sources as well...
  79. ncbi Identity of the immunomodulatory proteins from garlic (Allium sativum) with the major garlic lectins or agglutinins
    Fatima Clement
    Department of Biochemistry and Nutrition, Central Food Technological Research Institute CSIR Laboratory, Mysore, Karnataka State, India
    Int Immunopharmacol 10:316-24. 2010
    ....
  80. pmc Human macrophage-derived chemokine (MDC), a novel chemoattractant for monocytes, monocyte-derived dendritic cells, and natural killer cells
    R Godiska
    ICOS Corporation, Bothell, Washington 98021, USA
    J Exp Med 185:1595-604. 1997
    ..Unlike most other CC chemokines, MDC is encoded on human chromosome 16. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes...
  81. ncbi In vitro poly(ADP-ribosyl)ated histones H1a and H1t modulate rat testis chromatin condensation differently
    M R Faraone-Mennella
    Dipartimento di Chimica Organica e Biologica, Universita Federico II, 16 80134 Napoli, Italy
    J Cell Biochem 76:20-9. 1999
    ..the poly(ADP-ribosyl)ated proteins were purified from the native forms by means of phenyl boronic agarose chromatography. The results from both analyses were in agreement and showed qualitative differences with regard to the ..
  82. pmc Phosphodiesterase inhibition in ventricular cardiomyocytes from guinea-pig hearts
    T Bethke
    Abteilung Allgemeine Pharmakologie, , , Germany
    Br J Pharmacol 107:127-33. 1992
    ....
  83. ncbi Trypanosoma brucei dihydrofolate reductase-thymidylate synthase: gene isolation and expression and characterization of the enzyme
    F Gamarro
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143, USA
    Mol Biochem Parasitol 72:11-22. 1995
    ..The T. brucei DHFR has Ki values for antimicrobial antifolates pyrimethamine and trimethoprim which are significantly lower than the closely related T. cruzi or L. major DHFRs or than human DHFR...
  84. pmc Binding characteristics of reduced hepatic receptors for acetylated low-density lipoprotein and maleylated bovine serum albumin
    E Ottnad
    Department of Medicine, University of Heidelberg, Federal Republic of Germany
    Biochem J 265:689-98. 1990
    ....
  85. ncbi The unique enzymatic function of field bean (Dolichos lablab) D-galactose specific lectin: a polyphenol oxidase
    Santosh R Kanade
    Department of Protein Chemistry and Technology, Central Food Technological Research Institute, Council of Scientific and Industrial Research CSIR, Mysore, India
    Glycoconj J 26:535-45. 2009
    ..The complementarity of these two simultaneous and independent powerful defense mechanisms exhibited by a single protein renders it a candidate gene for the development of inbuilt plant protection...
  86. ncbi Purification and characterization of a rat liver bile acid coenzyme A ligase from rat liver microsomes
    J B Wheeler
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham 35294, USA
    Arch Biochem Biophys 348:15-24. 1997
    ..Chenodeoxycholyl-CoA and 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestan-27-oyl-CoA were confirmed as reaction products of purified rBAL by HPLC-electrospray ionization mass spectrometry...
  87. ncbi Characterization of the dermatan sulfate proteoglycans, DS-PGI and DS-PGII, from bovine articular cartilage and skin isolated by octyl-sepharose chromatography
    H U Choi
    Orthopedic Research Laboratories, Montefiore Medical Center, Bronx, New York 10467
    J Biol Chem 264:2876-84. 1989
    ....
  88. ncbi Purification and characterization of human brain ribonuclease inhibitor
    D Nadano
    Department of Legal Medicine, Fukui Medical School, Japan
    Arch Biochem Biophys 312:421-8. 1994
    ..In contrast to the abundance of RI in human brain tissue (about 0.08% (w/w) of total protein), RI was undetectable in human cerebrospinal fluid, suggesting that brain RI may not be a secreted protein...
  89. ncbi Butyrylcholinesterase activity and molecular components in thymus of healthy and merosin-deficient Lama2dy mice
    Luis F Sánchez del Campo
    Departamento de Bioquimica y Biologia Molecular A, Universidad de Murcia, E 30071 Espinardo, Murcia, Spain
    Neurochem Int 50:531-9. 2007
    ..Sedimentation analyses and phenyl-agarose chromatography showed that thymus contained amphiphilic BuChE monomers (G(1)(A),44%) and dimers (G(2)(A),33%), and ..
  90. ncbi Site-directed mutagenesis of the arginine-glycine-aspartic acid in vitronectin abolishes cell adhesion
    R C Cherny
    Department of Medicine, University of Washington, Seattle 98195
    J Biol Chem 268:9725-9. 1993
    ..All recombinant proteins were isolated by heparin-agarose chromatography and tested for interaction with three known vitronectin receptors, namely, alpha IIIb beta 3 on thrombin-..
  91. ncbi Characterization of tubulin from Brugia malayi and Brugia pahangi
    L Tang
    Institute of Parasitology, McGill University, Macdonald College, Ste Anne de Bellevue, Quebec, Canada
    Mol Biochem Parasitol 32:145-52. 1989
    ..pahangi extracts, respectively. Tubulins from Brugia nematodes have been partially purified by polylysine agarose chromatography and with taxol...
  92. ncbi Large-scale expression, refolding, and purification of the catalytic domain of human macrophage metalloelastase (MMP-12) in Escherichia coli
    A A Parkar
    Respiratory and RA Disease Group, Aventi Pharmaceuticals, Bridgewater, New Jersey 08807 0800, USA
    Protein Expr Purif 20:152-61. 2000
    ..This purification procedure enables the production of 23 mg of protein per liter of E. coli culture and is amenable to large-scale protein production for structural studies...
  93. ncbi Purification and sequence analysis of two rat tissue inhibitors of metalloproteinases
    W T Roswit
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63130
    Arch Biochem Biophys 292:402-10. 1992
    ..Exposure of UMR 106-01 cells to 10(-7) M parathyroid hormone resulted in approximately a 40% increase in total inhibitor production over basal levels...
  94. ncbi Isolation and characterization of biologically active lectin from Korean mistletoe, Viscum album var. Coloratum
    H S Lee
    College of Pharmacy, Wonkwang University, Iksan, Korea
    Cell Mol Life Sci 55:679-82. 1999
    ..7 kDa and to consist of two subunits of 30.6 kDa and 32.5 kDa. It was a basic protein with isoelectric points of 9.4 and 9.6 by capillary isoelectric focusing and was cytotoxic to Molt4 cell...
  95. ncbi Soluble tubulin complexes in oocytes of the common leopard frog, Rana pipiens, contain gamma-tubulin
    C A Lessman
    Department of Microbiology and Molecular Cell Sciences, The University of Memphis, Memphis, Tennessee 38152 6041, USA
    Mol Reprod Dev 60:128-36. 2001
    ..While the gamma-tubulin ring complexes of vertebrate eggs have been described previously, this is the first report biochemically characterizing soluble gamma-tubulin complexes in vertebrate ovarian oocytes...
  96. ncbi Characterization of trichobakin, a type I ribosome-inactivating protein from Trichosanthes sp. Bac Kan 8-98
    P V Chi
    Institute of Biotechnology, National Center for Natural Science and Technology, Hoang Quoc Viet Road, Cau Giay, Hanoi, Vietnam
    Biotechnol Appl Biochem 34:85-92. 2001
    ..5 pM. The rRNA N-glycosidase activity of the protein was also proved at the above-mentioned concentration after rRNAs were treated with acid aniline...
  97. ncbi Human skin mast cell carboxypeptidase: functional characterization, cDNA cloning, and genealogy
    M Natsuaki
    Department of Dermatology, University of California, San Francisco 94143 0536
    J Invest Dermatol 99:138-45. 1992
    ..Thus, in the carboxypeptidase gene family, MC-CPA displays a unique genealogy and several amino acid replacements in its S1' binding pocket that result in substrate specificity quite similar to bovine CPA...
  98. ncbi Two roles for mu-crystallin: a lens structural protein in diurnal marsupials and a possible enzyme in mammalian retinas
    L Segovia
    Section on Molecular Structure and Function, National Eye Institute, National Institutes of Health, Bethesda, MD, USA
    Mol Vis 3:9. 1997
    ..Here we examine the distribution of mu-crystallin among species and its localization in the eye in placental mammals. We also compare its sequence and ligand binding characteristics with those of known enzymes...
  99. ncbi Alteration of the fibrinogen molecule and its phosphorylation state in myocardial infarction patients undergoing thrombolytic treatment
    A C Haglund
    Department of Medical Biochemistry and Microbiology, Biomedical Centre, University of Uppsala, University Hospital, Uppsala, Sweden
    Thromb Res 98:147-56. 2000
    Fibrinogen was purified by protamine-agarose chromatography from plasma from three patients after their submission to hospital due to acute myocardial infarction...
  100. ncbi Binding of hepatic lipase to heparin. Identification of specific heparin-binding residues in two distinct positive charge clusters
    R A Sendak
    Divisions of Nutritional and Biological Sciences, Cornell University, Ithaca, NY 14853, USA
    J Lipid Res 41:260-8. 2000
    ..This is different for LPL: mutations in the C-terminal binding domain (Cluster 4) cause a more significant shift in the salt required for elution from heparin-Sepharose than mutations in the N-terminal domain (Cluster 1)...
  101. ncbi Interaction of calreticulin with protein disulfide isomerase
    S Baksh
    Medical Research Council Group in Molecular Biology of Membrane, University of Alberta, Edmonton, Canada
    J Biol Chem 270:31338-44. 1995
    ..The PDI-calreticulin complex can be dissociated by Zn(2+)-iminodiacetate-substituted Sepharose-agarose chromatography, suggesting that these interactions may be Zn2+-dependent...

Research Grants16

  1. X-LINKED OCULAR ALBINISM & RETINITIS PIGMENTOSA
    Anand Swaroop; Fiscal Year: 1993
    ..RNA from human B-lymphocytes, and by subsequently separating the specific cDNAs by streptavidin-agarose chromatography. The subtracted cDNA clones enriched for eye/retina-specific messages will be selected for sequences ..
  2. ROLE OF PHOSPHOLIPIDS IN MINERALIZATION OF OSTEOID
    David Howell; Fiscal Year: 1992
    ..With increased understanding of how growth plate calcification occurs, it is hoped that additional sites for therapeutic intervention in OA will be found...
  3. PHOSPHOLIPIDS AND MINERALIZATION OF OSTEOID
    David Howell; Fiscal Year: 1999
    ..With increased understanding of how growth plate calcification occurs, it is hoped that additional sites for therapeutic intervention in OA will be found. ..
  4. LEUKOCYTE INTERACTIONS WITH EXTRACELLULAR MATRIX
    ARTHUR MERCURIO; Fiscal Year: 1999
    ....
  5. PCP RECEPTOR CLASSES: STRUCTURE & THERAPEUTIC ASPECTS
    MORDECHAI SOKOLOVSKY; Fiscal Year: 1990
    ..of the four putative NMDA PCP- receptor polypeptides already purified from the rat forebrain by amino-PCP agarose chromatography. 2...
  6. ENZYMATIC N-METHYLATION OF CALMODULIN
    FRANK SIEGEL; Fiscal Year: 1993
    ..This research is relevant to basic biochemical processes in cancer and normal developmental processes...
  7. BIOCHEMISTRY OF NORMAL AND LEUKEMIC LYMPHOCYTES
    CREED ABELL; Fiscal Year: 1980
    ....
  8. QUANTITATIVE MODEL OF EGF-CELL INTERACTIONS
    H Wiley; Fiscal Year: 1992
    ..of EGF on cell surface composition will use double labeling of cell surface components followed by lectin-agarose chromatography and multi-step gel electrophoresis...
  9. Tumor-reactive IgG in the diagnosis of ovarian cancer
    Douglas Taylor; Fiscal Year: 2005
    ..Ultimately, antigenic proteins, linked with histologic type and stage of disease, will be combined into the development of protein arrays to screen for circulating reactive IgG as an early diagnostic test. ..
  10. T Cell Memory to Respiratory Viral Infections
    Shahram Salek Ardakani; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  11. IMMUNOLOGICAL ROLE OF THE STRESS INDUCIBLE HSP70
    Antoine Menoret; Fiscal Year: 2002
    ..The possibility that the inducible HSPs have a superior immunogenicity than the constitutively expressed HSPs has not been explored so far and might be of considerable benefit for public health. ..
  12. METALLO BETA LACTAMASE FROM X MALTOPHILIA
    Michael Crowder; Fiscal Year: 2002
    ....
  13. HBF VARIANTS FOR GENE THERAPY OF SICKLE CELL DISEASE
    Kazuhiko Adachi; Fiscal Year: 2005
    ....
  14. Zn(II) metallochaperones in E. coli
    Michael Crowder; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  15. Left Ventricular Remodeling by Matrix Metabolism
    Richard Lee; Fiscal Year: 2004
    ..We will use the emerging technology of proteomics to identify substrates for metalloproteinases that may participate in LV remodeling. ..