gel chromatography

Summary

Summary: Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.

Top Publications

  1. ncbi Analysis of the regulation of the molecular chaperone Hsp26 by temperature-induced dissociation: the N-terminal domail is important for oligomer assembly and the binding of unfolding proteins
    Thusnelda Stromer
    Institut fur Organische Chemie and Biochemie, Technische Universitat Munchen, Lichtenbergstrasse 4, 85747 Garching, Germany
    J Biol Chem 279:11222-8. 2004
  2. ncbi Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins
    Toshimitsu Kawate
    Department of Biochemistry and Molecular Biophysics, Columbia University, 650 West 168 th Street, New York, New York 10032, USA
    Structure 14:673-81. 2006
  3. pmc GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae
    David Drew
    Membrane Protein Crystallography Group, Division of Molecular Biosciences, Department of Life Sciences, Imperial College of London, London SW7 2AZ, UK
    Nat Protoc 3:784-98. 2008
  4. ncbi Folding of the striated muscle myosin motor domain
    Diana Chow
    Department of Pathology and Laboratory Medicine, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854, USA
    J Biol Chem 277:36799-807. 2002
  5. ncbi Antibody response to aggregated human interferon alpha2b in wild-type and transgenic immune tolerant mice depends on type and level of aggregation
    Suzanne Hermeling
    Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences UIPS, Utrecht University, P O BOX 80082, 3508 TB Utrecht, The Netherlands
    J Pharm Sci 95:1084-96. 2006
  6. ncbi Combination of SEC/MALS experimental procedures and theoretical analysis for studying the solution properties of macromolecules
    M Pilar Tarazona
    Departamento de Quimica Fisica, Universidad de Alcala, 28871 Alcala de Henares, Spain
    J Biochem Biophys Methods 56:95-116. 2003
  7. ncbi A critical review of methods for size characterization of non-particulate protein aggregates
    John S Philo
    Alliance Protein Laboratories, Thousand Oaks, CA, USA
    Curr Pharm Biotechnol 10:359-72. 2009
  8. pmc Association properties of betaB1- and betaA3-crystallins: ability to form heterotetramers
    May P Chan
    National Eye Institute and National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 47:11062-9. 2008
  9. ncbi Structural characterization and immunogenicity in wild-type and immune tolerant mice of degraded recombinant human interferon alpha2b
    Suzanne Hermeling
    Central Laboratory Animal Institute, Utrecht University, Utrecht, The Netherlands
    Pharm Res 22:1997-2006. 2005
  10. ncbi Structural and enzymatic characterization of the isoamylase1 homo-oligomer and the isoamylase1-isoamylase2 hetero-oligomer from rice endosperm
    Yoshinori Utsumi
    Department of Biological Production, Akita Prefectural University, 241 7 Kaidobata Nishi, Shimoshinjyo Nakano, Akita City, 010 0195, Japan
    Planta 225:75-87. 2006

Detail Information

Publications277 found, 100 shown here

  1. ncbi Analysis of the regulation of the molecular chaperone Hsp26 by temperature-induced dissociation: the N-terminal domail is important for oligomer assembly and the binding of unfolding proteins
    Thusnelda Stromer
    Institut fur Organische Chemie and Biochemie, Technische Universitat Munchen, Lichtenbergstrasse 4, 85747 Garching, Germany
    J Biol Chem 279:11222-8. 2004
    ....
  2. ncbi Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins
    Toshimitsu Kawate
    Department of Biochemistry and Molecular Biophysics, Columbia University, 650 West 168 th Street, New York, New York 10032, USA
    Structure 14:673-81. 2006
    ..We show the application of this precrystallization screening to four membrane proteins derived from prokaryotic or eukaryotic organisms...
  3. pmc GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae
    David Drew
    Membrane Protein Crystallography Group, Division of Molecular Biosciences, Department of Life Sciences, Imperial College of London, London SW7 2AZ, UK
    Nat Protoc 3:784-98. 2008
    ..This protocol also details the purification of targets that pass our quality criteria, and can be scaled up for a large number of eukaryotic membrane proteins in either an academic, structural genomics or commercial environment...
  4. ncbi Folding of the striated muscle myosin motor domain
    Diana Chow
    Department of Pathology and Laboratory Medicine, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854, USA
    J Biol Chem 277:36799-807. 2002
    ..The expression studies support this conclusion and demonstrate that these factors are optimized in muscle cells...
  5. ncbi Antibody response to aggregated human interferon alpha2b in wild-type and transgenic immune tolerant mice depends on type and level of aggregation
    Suzanne Hermeling
    Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences UIPS, Utrecht University, P O BOX 80082, 3508 TB Utrecht, The Netherlands
    J Pharm Sci 95:1084-96. 2006
    ..In conclusion, the transgenic mouse model can be used to screen rhIFNalpha2b formulations for low levels of immunogenic aggregates obtained under accelerated storage conditions...
  6. ncbi Combination of SEC/MALS experimental procedures and theoretical analysis for studying the solution properties of macromolecules
    M Pilar Tarazona
    Departamento de Quimica Fisica, Universidad de Alcala, 28871 Alcala de Henares, Spain
    J Biochem Biophys Methods 56:95-116. 2003
    ..Theoretical values obtained with the RIS model are in good agreement with the experimental results...
  7. ncbi A critical review of methods for size characterization of non-particulate protein aggregates
    John S Philo
    Alliance Protein Laboratories, Thousand Oaks, CA, USA
    Curr Pharm Biotechnol 10:359-72. 2009
    ..3 microns. The physical principles of the biophysical methods are briefly described and examples of data for real samples and how that data is interpreted are given to help clarify capabilities and weaknesses...
  8. pmc Association properties of betaB1- and betaA3-crystallins: ability to form heterotetramers
    May P Chan
    National Eye Institute and National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 47:11062-9. 2008
    ..These findings suggest a unique role for betaB1-crystallin in promoting higher-order crystallin association in the lens...
  9. ncbi Structural characterization and immunogenicity in wild-type and immune tolerant mice of degraded recombinant human interferon alpha2b
    Suzanne Hermeling
    Central Laboratory Animal Institute, Utrecht University, Utrecht, The Netherlands
    Pharm Res 22:1997-2006. 2005
    ..This study was conducted to study the influence of protein structure on the immunogenicity in wild-type and immune tolerant mice of well-characterized degradation products of recombinant human interferon alpha2b (rhIFNalpha2b)...
  10. ncbi Structural and enzymatic characterization of the isoamylase1 homo-oligomer and the isoamylase1-isoamylase2 hetero-oligomer from rice endosperm
    Yoshinori Utsumi
    Department of Biological Production, Akita Prefectural University, 241 7 Kaidobata Nishi, Shimoshinjyo Nakano, Akita City, 010 0195, Japan
    Planta 225:75-87. 2006
    ..These results suggest that the hetero-oligomer plays a predominant role in the amylopectin biosynthesis in rice endosperm although the homo-oligomer can complement the function of the hetero-oligomer at least to some extent...
  11. ncbi Micelle-associated protein in epoetin formulations: aA risk factor for immunogenicity?
    Suzanne Hermeling
    Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences UIPS, Utrecht, The Netherlands
    Pharm Res 20:1903-7. 2003
    ..The aim of this study was to detect the mechanism by which the new formulation may induce these antibodies...
  12. pmc An efficient strategy for high-throughput expression screening of recombinant integral membrane proteins
    Said Eshaghi
    Department of Biochemistry and Biophysics, Stockholm University, Roslagstullsbacken 15, AlbaNova University Center, SE 114 21 Stockholm, Sweden
    Protein Sci 14:676-83. 2005
    ..The presented strategy demonstrates the usefulness of HTP technologies for membrane protein production, and the feasibility of large-scale programs for elucidation of structure and function of bacterial integral membrane proteins...
  13. pmc Analysis of the unwinding activity of the dimeric RECQ1 helicase in the presence of human replication protein A
    Sheng Cui
    International Centre for Genetic Engineering and Biotechnology, Padriciano 99, I 34012 Trieste, Italy
    Nucleic Acids Res 32:2158-70. 2004
    ..In addition, our far western, ELISA and co-immunoprecipitation experiments demonstrate that RECQ1 physically interacts with the 70 kDa subunit of hRPA and that this interaction is not mediated by DNA...
  14. ncbi Human peroxiredoxin 1 and 2 are not duplicate proteins: the unique presence of CYS83 in Prx1 underscores the structural and functional differences between Prx1 and Prx2
    Weonsup Lee
    Department of Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA
    J Biol Chem 282:22011-22. 2007
    ..These findings are consistent with the hypothesis that human Prx1 and Prx2 possess unique functions and regulatory mechanisms and that Cys(83) bestows a distinctive identity to Prx1...
  15. ncbi Using light scattering to determine the stoichiometry of protein complexes
    Jeremy Mogridge
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada
    Methods Mol Biol 261:113-8. 2004
    ..The determination of the molecular weight of an anthrax toxin complex will be examined to illustrate how multiangle laser light scattering can be used to determine the stoichiometry of protein complexes...
  16. ncbi A model for ATP hydrolysis-dependent binding of cohesin to DNA
    Stefan Weitzer
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    Curr Biol 13:1930-40. 2003
    ..The mechanism by which cohesin binds to DNA, and how sister chromatid cohesion is established, is unknown...
  17. pmc Laser light-scattering evidence for an altered association of beta B1-crystallin deamidated in the connecting peptide
    Michael J Harms
    Oregon Health and Science University, Portland, OR 97239, USA
    Protein Sci 13:678-86. 2004
    ..Deamidation at Gln146, but not Asn157, led to an increased tendency of beta B1 to aggregate upon heating. We conclude that deamidation creates unique effects depending upon where the deamidation is introduced in the crystallin structure...
  18. ncbi Characterization of loaded liposomes by size exclusion chromatography
    Cécile Grabielle-Madelmont
    Equipe Physico Chimie des Systèmes Polyphasés, UMR CNRS 8612, Universite Paris Sud, Châtenay Malabry Cedex 92296, France
    J Biochem Biophys Methods 56:189-217. 2003
    ..up to large molecules (proteins, peptides and nucleic acids) in liposomes...
  19. ncbi Characterization and cDNA cloning of aminopeptidase A from the venom of Gloydius blomhoffi brevicaudus
    Yuko Ogawa
    Institute of Medicinal Chemistry, Hoshi University, Shinagawa ku, Tokyo 142 8501, Japan
    Toxicon 49:1172-81. 2007
    ..The deduced amino acid sequence shows approximately 60% sequence identity to mammalian APA sequences. This is the first study to report the primary structure of APA from a reptile...
  20. pmc Structure of the cyclomodulin Cif from pathogenic Escherichia coli
    Yun Hsu
    Laboratory of Structural Microbiology, Rockefeller University, New York, NY 10021, USA
    J Mol Biol 384:465-77. 2008
    ..Finally, we demonstrate that irreversible cysteine protease inhibitors do not abolish the Cif cytopathic effect, suggesting that another enzymatic activity may underlie the biological activity of this virulence factor...
  21. ncbi Structure-based analysis of the beta8 interactive sequence of human alphaB crystallin
    Joy G Ghosh
    Biomolecular Structure and Design, University of Washington, Seattle, Washington 98195 7420, USA
    Biochemistry 45:9878-86. 2006
    ..The results established the beta3-beta8-beta9 surface of the alpha crystallin core domain as an interface for complex assembly and chaperone activity...
  22. ncbi Changes in cell wall composition of three Fragaria x ananassa cultivars with different softening rate during ripening
    H G Rosli
    Instituto de Investigaciones Biotecnologicas, Instituto Tecnológico de Chascomús IIB INTECH, UNSAM CONICET, Camino Circunvalación Laguna Km 6, 7130 Chascomus, Argentina
    Plant Physiol Biochem 42:823-31. 2004
    ..WSP showed depolymerization only in Toyonaka cultivar, while HSP showed depolymerization in Pajaro and Toyonaka cultivars. A slight depolymerization was observed in hemicelluloses extracted from any of three cultivars...
  23. ncbi Assignment of the binding site for haptoglobin on apolipoprotein A-I
    Maria Stefania Spagnuolo
    Dipartimento di Fisiologia Generale ed Ambientale, Universita di Napoli Federico II, Via Mezzocannone 8, 80134 Napoli, Italia
    J Biol Chem 280:1193-8. 2005
    ..Synthetic peptides, able to displace Hpt from ApoA-I without altering its property of binding hemoglobin, might be used for treatment of diseases associated with defective LCAT function...
  24. ncbi Comparison of two gel filtration chromatographic methods for the purification of Lily symptomless virus
    Ruoyu Wang
    Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, PR China
    J Virol Methods 139:125-31. 2007
    ..Furthermore, it can provide an economical and efficient way to obtain purified products for the preparation of antibodies for serological diagnosis or LSV infection and related investigations...
  25. ncbi Exosome-like vesicles in Gloydius blomhoffii blomhoffii venom
    Yuko Ogawa
    Institute of Medicinal Chemistry, Hoshi University, Shinagawa ku, Tokyo 142 8501, Japan
    Toxicon 51:984-93. 2008
    ..The role of these vesicles is still unknown, but they may affect blood pressure and glucose homeostasis following envenomation...
  26. ncbi Fragmentation pattern of mucins in normal and diseased gastric mucosae: a glycoprotein fractionates with gastric mucins purified from mucosal scrapings of cancer and peptic ulcer patients
    A S Mall
    Department of Surgery and MRC Liver Research Centre, University of Cape Town Medical School, Cape Town, South Africa
    Digestion 60:216-26. 1999
    ..The aim of this study was a biochemical characterisation of gastric mucins in this disease, compared with gastric ulceration and controls from transplant donors...
  27. ncbi Synthesis, purification and characterization of recombinant glycosylated human prolactin (G-hPRL) secreted by cycloheximide-treated CHO cells
    S R Heller
    Biotechnology Department, IPEN CNEN, Av Lineu Prestes, 2242, Cidade Universitaria, 05508 900 Sao Paulo, Brazil
    J Biotechnol 145:334-40. 2010
    ..Our results show that addition of cycloheximide can be an important strategy for increasing glycosylated protein production, facilitating the purification and characterization of these isoforms...
  28. ncbi Gel chromatography and analytical ultracentrifugation to determine the extent of detergent binding and aggregation, and Stokes radius of membrane proteins using sarcoplasmic reticulum Ca2+-ATPase as an example
    Marc le Maire
    CEA, Institut de Biologie et Technologies de Saclay, F 91191 Gif sur Yvette, France
    Nat Protoc 3:1782-95. 2008
    ..The whole time for these experiments is 24 h. Data analysis of analytical ultracentrifugation requires a couple of days...
  29. ncbi Deamidation of human beta B1 alters the elongated structure of the dimer
    K J Lampi
    Department of Oral Molecular Biology, Oregon Health Sciences University, Portland, OR 97201, USA
    Exp Eye Res 72:279-88. 2001
    ..Truncation caused abnormal column interactions suggesting an altered conformation. These changes are important because truncation and deamidation occur extensively in aging human lenses and may be important for senile cataract formation...
  30. ncbi The solution structure of the simian foamy virus protease reveals a monomeric protein
    Maximilian J Hartl
    Lehrstuhl für Struktur und Chemie der Biopolymere, Universitat Bayreuth, 95440 Bayreuth, Germany
    J Mol Biol 381:141-9. 2008
    ..Dimerization might therefore be triggered by additional viral or cellular factors...
  31. ncbi The p73 DNA binding domain displays enhanced stability relative to its homologue, the tumor suppressor p53, and exhibits cooperative DNA binding
    Seema Patel
    Department of Biochemistry, School of Biomedical and Health Sciences, King s College London, 150 Stamford Street, London SE1 9NH, United Kingdom
    Biochemistry 47:3235-44. 2008
    ..These stability and DNA binding data for p73 in vitro enhance and complement our understanding of the role of the p73 protein in vivo and could be exploited in designing strategies for cancer therapy in places where p53 is mutated...
  32. ncbi Multi-grams scale purification of xanthanolides from Xanthium macrocarpum. Centrifugal partition chromatography versus silica gel chromatography
    Benoit Pinel
    Laboratoire SONAS EA 921, UFR des Sciences Pharmaceutiques et Ingenierie de la Sante, 16 Bd Daviers, 49100 Angers, France
    J Chromatogr A 1151:14-9. 2007
    ..macrocarpum by silica gel chromatography and for the first time by liquid/liquid chromatography (counter-current chromatography/centrifugal partition ..
  33. ncbi N2-methylation of guanosine at position 10 in tRNA is catalyzed by a THUMP domain-containing, S-adenosylmethionine-dependent methyltransferase, conserved in Archaea and Eukaryota
    Jean Armengaud
    Commissariat à l Energie Atomique VALRHO, DSV DIEP SBTN, Service de Biochimie post génomique and Toxicologie Nucléaire, F 30207 Bagnols sur Ceze, France
    J Biol Chem 279:37142-52. 2004
    ..The co-occurrence of these two guanosine dimethyltransferases in both Archaea and Eukaryota but not in Bacteria is a hallmark of distinct tRNAs maturation strategies between these domains of life...
  34. ncbi Analysis of Ras:RasGEF interactions by phage display and static multi-angle light scattering
    Holger Sondermann
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Methods 37:197-202. 2005
    ..This technique also provides information about conformational homogeneity which can be used as a quality..
  35. ncbi Determination of lignin by size exclusion chromatography using multi angle laser light scattering
    Aarti V Gidh
    National Bioenergy Center, National Renewable Energy Laboratory, 1617 Cole Blvd, Golden, CO 80401, USA
    J Chromatogr A 1114:102-10. 2006
    ..It is very important to take this loss in apparent concentration due to aggregation into consideration before collecting reliable depolymerization data...
  36. ncbi Development of HSA-free formulations for a hydrophobic cytokine with improved stability
    Andrea Hawe
    Ludwig Maximilians University Munich, Department of Pharmacy, Pharmaceutical Technology and Biopharmaceutics, Munich, Germany
    Eur J Pharm Biopharm 68:169-82. 2008
    ..Liquid formulations at pH 3.5-4.0 and lyophilized formulations at pH 4.0-5.0 were most stable during 6 months at 2-8 degrees C...
  37. ncbi Purification of immunoglobulins from chicken sera by thiophilic gel chromatography
    C C Constantinoiu
    School of Veterinary Science, University of Queensland, Brisbane, Queensland, 4072, Australia
    Poult Sci 86:1910-4. 2007
    ..Thiophilic gel chromatography has been successfully used to purify IgY from chicken egg yolk, but the technology has not previously been ..
  38. pmc Purification and structural studies on the complement-system control protein beta 1H (Factor H)
    R B Sim
    Biochem J 205:285-93. 1982
    ..The proteolytic fragments of Factor H are compared with those of other proteins (C4b-binding protein and erythrocyte C3b-receptor) that act as cofactors for C3b-inactivator...
  39. ncbi Molar-mass characterization of cationic polymers for gene delivery by aqueous size-exclusion chromatography
    Xulin Jiang
    Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences UIPS, Utrecht University, P O Box 80 082, 3508 TB, Utrecht, The Netherlands
    Pharm Res 23:595-603. 2006
    ..This study was performed to develop a reliable aqueous size-exclusion chromatography (SEC) method to obtain the absolute molar masses and distributions of various cationic polymers used in gene delivery...
  40. pmc Interaction between the C-terminal region of human myelin basic protein and calmodulin: analysis of complex formation and solution structure
    Viivi Majava
    Department of Biochemistry, University of Oulu, Oulu, Finland
    BMC Struct Biol 8:10. 2008
    ..In this study, we focus on a 19-mer synthetic peptide from the predicted calmodulin-binding segment near the C-terminus of human myelin basic protein...
  41. ncbi "Natively unfolded" VPg is essential for Sesbania mosaic virus serine protease activity
    Panayampalli Subbian Satheshkumar
    Department of Biochemistry, Indian Institute of Science, Bangalore
    J Biol Chem 280:30291-300. 2005
    ..Thus, VPg is an activator of protease in Sesbania mosaic virus, and probably by this mechanism, the polyprotein processing could be regulated in planta...
  42. ncbi Structural biochemistry of ATP-driven dimerization and DNA-stimulated activation of SMC ATPases
    Alfred Lammens
    Gene Center and Institute of Biochemistry, University of Munich, Feodor Lynen Strasse 25, 81377 Munich, Germany
    Curr Biol 14:1778-82. 2004
    ..Our results suggest that stimulation of the SMC ATPase activity may be a specific feature to regulate the ATP-driven assembly and disassembly of SMC proteins...
  43. pmc Mutation of interfaces in domain-swapped human betaB2-crystallin
    Myron A Smith
    Department of Crystallography, Institute of Structural and Molecular Biology, Birkbeck College, University of London, London WC1E 7HX, United Kingdom
    Protein Sci 16:615-25. 2007
    ..These are desirable attributes for proteins that have to exist at very high concentration for a very long time...
  44. pmc A high-throughput method for membrane protein solubility screening: the ultracentrifugation dispersity sedimentation assay
    Daniel A P Gutmann
    Membrane Protein Crystallography Group, Division of Molecular Biosciences, Imperial College London, UK
    Protein Sci 16:1422-8. 2007
    ..The implications of our results for membrane protein crystallization prescreening are discussed...
  45. ncbi The Cfr10I restriction enzyme is functional as a tetramer
    V Siksnys
    Institute of Biotechnology, Lithuania
    J Mol Biol 291:1105-18. 1999
    ..These data are consistent with the tetramer being a functionally important form of Cfr10I...
  46. ncbi Association behaviour of human betaB1-crystallin and its truncated forms
    O A Bateman
    Birkbeck College, Department of Crystallography, Malet Street, London, WC1E 7HX, UK
    Exp Eye Res 73:321-31. 2001
    ..Good crystals have been grown of a truncated version of human betaB1-crystallin using an in vitro cleavage protocol...
  47. pmc Identification of natural rubber and characterization of rubber biosynthetic activity in fig tree
    H Kang
    Kumho Life and Environmental Science Laboratory, 1 Oryong dong, Puk Gu, Kwangju, 500 712 Korea
    Plant Physiol 123:1133-42. 2000
    ....
  48. ncbi Lens proteomics: the accumulation of crystallin modifications in the mouse lens with age
    Yoji Ueda
    Department of Animal Sciences, Oregon State University, Corvallis, Oregon, USA
    Invest Ophthalmol Vis Sci 43:205-15. 2002
    ..To identify modified crystallins associated with aging of lens and produce two-dimensional electrophoresis (2-DE) proteome maps of crystallins in mouse lens...
  49. ncbi The C-terminal domain of the measles virus nucleoprotein is intrinsically disordered and folds upon binding to the C-terminal moiety of the phosphoprotein
    Sonia Longhi
    Architecture et Fonction des Macromolecules Biologiques, UMR 6098 CNRS et Université Aix Marseille I et II, ESIL, Campus de Luminy, 13288 Marseille Cedex 09, France
    J Biol Chem 278:18638-48. 2003
    ..These results contribute to the study of the emerging field of natively unfolded proteins...
  50. ncbi Comparative study of protein molecular weights by size-exclusion chromatography and laser-light scattering
    A Oliva
    , Facultad de Farmacia, Universidad de La Laguna, 38200, Tenerife, Spain
    J Pharm Biomed Anal 25:833-41. 2001
    ..The method's reproducibility was accepted by assuming a precision of 98% and a 1% loss in precision...
  51. ncbi Characterization of transport through the periplasmic histidine permease using proteoliposomes reconstituted by dialysis
    C E Liu
    Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    J Biol Chem 272:859-66. 1997
    ..The inhibition by histidine implies that the membrane-bound complex HisQ/M/P carries a substrate-binding site. The reconstituted permease activity corresponds to about 40-70% turnover rate of the in vivo rate of transport...
  52. ncbi Hepatic Ins(1,3,4,5)P4 3-phosphatase is compartmentalized inside endoplasmic reticulum
    N Ali
    Laboratory of Cellular and Molecular Pharmacology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709
    J Biol Chem 268:6161-7. 1993
    ..Thus, hepatic 3-phosphatase has a highly restricted access to inositol polyphosphates in vivo that needs to be accounted for in the determination of the physiological role of this enzyme...
  53. ncbi Properties of thiol-specific anti-oxidant protein or calpromotin in solution
    P Kristensen
    The August Krogh Institute, University of Copenhagen, Denmark
    Biochem Biophys Res Commun 262:127-31. 1999
    ..5. Dissociation of the complex was also complete at 2.5 M urea, where no unfolding of the peptide chains was detectable...
  54. ncbi The N-terminal domain of the phosphoprotein of Morbilliviruses belongs to the natively unfolded class of proteins
    David Karlin
    Architecture et Fonction des Macromolecules Biologiques, UMR 6098 CNRS, Université Aix Marseille I et II, ESIL, Campus de Luminy, Marseille Cedex 09, France
    Virology 296:251-62. 2002
    ..quot; Using limited proteolysis in the presence of trifluoroethanol, we identified residues 27 to 38 as a putative secondary structure element of PNT arising upon induced folding...
  55. ncbi Rapid migration in gel filtration of the Cf-4 and Cf-9 resistance proteins is an intrinsic property of Cf proteins and not because of their association with high-molecular-weight proteins
    Renier A L van der Hoorn
    Wageningen University, Laboratory of Phytopathology, Binnenhaven 5, 6709 PD, Wageningen, The Netherlands
    Plant J 35:305-15. 2003
    ..Taken together, these results suggest that caution should be taken when interpreting data obtained from gel filtration of LRR-containing proteins...
  56. ncbi A minimal exonuclease domain of WRN forms a hexamer on DNA and possesses both 3'- 5' exonuclease and 5'-protruding strand endonuclease activities
    Yu Xue
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Biochemistry 41:2901-12. 2002
    ..Thus, the active form of this minimal exonuclease fragment of human WRN appears to be a hexamer. The implications these results have on our understanding of hWRN's roles in DNA replication and repair are discussed...
  57. ncbi How bacterial ribosomal protein L20 assembles with 23 S ribosomal RNA and its own messenger RNA
    Sophie Raibaud
    Cristallographie and RMN Biologiques, UMR 8015 CNRS, Faculte de Pharmacie, 4 avenue de l Observatoire, 75006 Paris, France
    J Biol Chem 278:36522-30. 2003
    ....
  58. pmc Identification of human urinary trefoil factor 1 as a novel calcium oxalate crystal growth inhibitor
    Somchai Chutipongtanate
    Medical Molecular Biology Unit, Office for Research and Development, Siriraj Hospital, Mahidol University, Bangkok, Thailand
    J Clin Invest 115:3613-22. 2005
    ..These data indicate that TFF1 is a novel potent CaOx crystal growth inhibitor with a potential pathophysiological role in nephrolithiasis...
  59. ncbi The oligomerization state determines regulatory properties and inhibitor sensitivity of type 4 cAMP-specific phosphodiesterases
    Wito Richter
    Division of Reproductive Biology, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, California 94305 5317, USA
    J Biol Chem 279:30338-48. 2004
    ..Taken together, our data indicate that dimerization defines the properties of PDE4 enzymes and suggest a common structural and functional organization for all PDEs...
  60. ncbi Tissue kallikrein-binding protein is a serpin. I. Purification, characterization, and distribution in normotensive and spontaneously hypertensive rats
    J Chao
    Department of Biochemistry, Medical University of South Carolina, Charleston 29425
    J Biol Chem 265:16394-401. 1990
    ..The results indicate that rat kallikrein-binding protein belongs to the serpin superfamily and its level is significantly reduced in the spontaneously hypertensive rats...
  61. ncbi Characterization and cDNA cloning of monomeric lectins that correspond to the B-Chain of a type 2 ribosome-inactivating protein from the bark of Japanese elderberry (Sambucus sieboldiana)
    Maria Angeles Rojo
    Department of Biochemistry, National Institute of Agrobiological Resources, University of Tsukuba, Tsukuba, Ibaraki 305
    J Biochem 135:509-16. 2004
    ..Carbohydrate binding specificity and biological activity of these lectins are also reported...
  62. ncbi Identification and isolation of a 75-kDa inositol polyphosphate-5-phosphatase from human platelets
    C A Mitchell
    Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110
    J Biol Chem 264:8873-7. 1989
    ..The levels of inositol phosphates that act in calcium signalling are likely to be regulated by the interplay of these two enzymes both found in the same cell...
  63. ncbi The amino acid sequence of human plasma prealbumin
    Y Kanda
    J Biol Chem 249:6796-805. 1974
  64. ncbi Characterization of profilaggrin endoproteinase 1. A regulated cytoplasmic endoproteinase of epidermis
    K A Resing
    Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309, USA
    J Biol Chem 270:28193-8. 1995
    ..In contrast to the specific cleavage of mouse profilaggrin, PEP1 cleaved all linker regions of rat profilaggrin. Studies with phosphorylated P1 suggest that PEP1 specificity may be partly regulated by profilaggrin phosphorylation...
  65. ncbi Oligomeric structure and substrate induced inhibition of human cathepsin C
    I Dolenc
    Department of Biochemistry and Molecular Biology, J Stefan Institute, Ljubljana, Slovenia
    J Biol Chem 270:21626-31. 1995
    ..2, 6.8, and 7.7 are involved in substrate hydrolysis. Human cathepsin C thus appears to differ qualitatively from other cysteine proteinases of different origin...
  66. ncbi Cloning, purification, and properties of a novel NADH pyrophosphatase. Evidence for a nucleotide pyrophosphatase catalytic domain in MutT-like enzymes
    D N Frick
    Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218
    J Biol Chem 270:1529-34. 1995
    ..Although these metals support similar Vmax values at optimal metal concentration, the apparent Km for Mg2+ is 3.7 mM (at 1 mM NADH), whereas the apparent Km for Mn2+ at the same NADH concentration is 30 microM...
  67. ncbi Purification and characterization of a novel species of ubiquitin-carrier protein, E2, that is involved in degradation of non-"N-end rule" protein substrates
    N Blumenfeld
    Department of Biochemistry, Faculty of Medicine, Technion Israel Institute of Technology, Haifa
    J Biol Chem 269:9574-81. 1994
    ..The enzyme is also involved in the conjugation and degradation of the tumor suppressor protein p53...
  68. ncbi Functional analysis of multiple single-stranded DNA-binding proteins from Methanosarcina acetivorans and their effects on DNA synthesis by DNA polymerase BI
    Justin B Robbins
    Department of Animal Sciences, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    J Biol Chem 279:6315-26. 2004
    ..Although bacterial SSB and eukaryotic RPA have been shown to stimulate DNA synthesis by their cognate DNA polymerases, our findings provide the first in vitro biochemical evidence for the conservation of this property in an archaeon...
  69. ncbi Purification, characterization and functional cloning of inositol oxygenase from Cryptococcus
    Ulrike Kanter
    University of Frankfurt, Biocentre, Plant Molecular Biology, Marie Curie Strasse 9, 60439 Frankfurt, Germany
    Yeast 20:1317-29. 2003
    ..5 and a temperature optimum of 30 degrees C. Myo-inositol is the only accepted substrate with a Km of ca. 5 mM. The enzyme contains a Fe-centre but the enzyme activity is resistant to KCN...
  70. ncbi Proteolysis in heterocyst-forming cyanobacteria: characterization of a further enzyme with trypsin-like specificity, and of a prolyl endopeptidase from Anabaena variabilis
    U Strohmeier
    , Regensburg, Bundesrepublik Deutschland
    Z Naturforsch C 49:70-8. 1994
    ..A second enzyme cleaving at the carboxyl side of lysine and arginine, and a prolyl endopeptidase were detected, enriched and characterized. Both proteolytic enzymes appear to be located in the periplasm...
  71. ncbi Production, purification, and biochemical characterization of two beta-glucosidases from Sclerotinia sclerotiorum
    Smaali Mohamed Issam
    Biological Engineering Unit, National Institute of Applied Sciences and Technology, BP 676, 1080 Tunis Cedex, Tunisia
    Appl Biochem Biotechnol 111:29-40. 2003
    ..beta-glu1 was stable over a pH range of 3-8 and kept 50% of its activity after 30 min of heating at 60 degrees C without substrate. It was further characterized by studying the effect of some cations and various reagents on its activity...
  72. ncbi Evaluation by mutagenesis of the importance of 3 arginines in alpha, beta, and gamma subunits of human NAD-dependent isocitrate dehydrogenase
    Sambanthamurthy Soundar
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    J Biol Chem 278:52146-53. 2003
    ....
  73. ncbi Preparative alkaline urea gradient PAGE: application to purification of extraordinarily-stable disulfide-linked homodimer of human growth hormone
    Alexei L Grigorian
    Department of Biochemistry and Molecular Genetics, University of Alabama School of Medicine, Birmingham, AL, USA
    Electrophoresis 28:3829-36. 2007
    ..E. = 22.93 +/- 3.90 pM), indicating that is was conformationally intact. Alkaline urea gradient PAGE is a valuable tool for preparative separation of structurally similar proteins such as isoforms of the hGH family...
  74. ncbi Biochemical and catalytic properties of an endoxylanase purified from the culture filtrate of Sporotrichum thermophile
    Petros Katapodis
    Department of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Ave, Zografou Campus, 15700 Athens, Greece
    Carbohydr Res 338:1881-90. 2003
    ..The enzyme is irreversibly inhibited by series of omega-epoxyalkyl glycosides of D-xylopyranose. The results suggest that the endoxylanase from S. thermophile has catalytic properties similar to the enzymes belonging to family 11...
  75. ncbi Villin-type headpiece domains show a wide range of F-actin-binding affinities
    D Vardar
    Department of Physiology and Biophysics, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Cell Motil Cytoskeleton 52:9-21. 2002
    ..We conclude that not all headpiece domains are intrinsically F-actin-binding motifs, and suggest that the surface charge distribution may be an important element for F-actin recognition...
  76. ncbi Functional identity of a primer recognition protein as phosphoglycerate kinase
    H K Jindal
    Department of Biochemistry, University of Nebraska Medical Center, Omaha 68105
    J Biol Chem 265:6540-3. 1990
    ..For PRP activity, the two substrate binding sites of PGK are necessary in addition to the as yet unidentified PRP 1 polypeptide...
  77. ncbi Purification and characterization of extracellular matrix-degrading metalloproteinase, matrin (pump-1), secreted from human rectal carcinoma cell line
    K Miyazaki
    Kihara Institute for Biological Research, Yokohama City University, Kanagawa, Japan
    Cancer Res 50:7758-64. 1990
    ..Based on the fact that this enzyme is secreted extracellularly and degrades the matrix proteins, we propose the name "matrin" for this newly identified enzyme...
  78. ncbi Purification and partial amino acid sequences of phosphoinositide-specific phospholipase C of Drosophila eye
    S Toyoshima
    Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan
    J Biol Chem 265:14842-8. 1990
    ..The obtained sequences were found to be a part of the deduced amino acid sequences of cDNA which was suggested to be norpA gene...
  79. pmc Rat liver mitochondrial intermediate peptidase (MIP): purification and initial characterization
    F Kalousek
    Department of Genetics, Yale University School of Medicine, New Haven, CT 06510
    EMBO J 11:2803-9. 1992
    ..Zinc, cobalt and iron strongly inhibit MIP activity. This pattern of cation dependence and inhibition is not clearly consistent with that of any known family of proteases...
  80. ncbi Cleavage mechanism of ATP-dependent Lon protease toward ribosomal S2 protein
    Wataru Nishii
    School of Life Science, Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji, Tokyo 192 0392, Japan
    FEBS Lett 579:6846-50. 2005
    ..Furthermore, polyP changed not only the substrate preference but also the oligomeric structure of the enzyme...
  81. ncbi Colorectum cell-derived growth factor (CRDGF) is homologous to amphiregulin, a member of the epidermal growth factor family
    J M Culouscou
    Bristol Myers Squibb Pharmaceutical Research Institute, Seattle, WA 98121
    Growth Factors 7:195-205. 1992
    ..This indicates that the AR intracellular signalling pathway involves the activation of EGF-R kinase...
  82. ncbi Purification and characterization of proteinase In, a trypsin-like proteinase, in Escherichia coli
    M Kato
    Faculty of Pharmacy, Tokushima Bunri University, Japan
    Eur J Biochem 210:1007-14. 1992
    ..coli. Proteinase activity was found in the cytoplasmic fraction...
  83. ncbi Characterization of components of the anaerobic ribonucleotide reductase system from Escherichia coli
    R Eliasson
    Department of Biochemistry, Karolinska Institute, Stockholm, Sweden
    J Biol Chem 267:25541-7. 1992
    ..Micromolar concentrations of several chelators inhibit CTP reduction completely, suggesting the involvement of (a) transition metal(s)...
  84. ncbi Expression, purification and partial characterization of the Krüppel-associated box (KRAB) from the human ZNF2 protein
    Vincenzo Rivieccio
    Istituto di Genetica e Biofisica A Buzzati Traverso, Consiglio Nazionale delle Ricerche, Italy
    Protein Pept Lett 12:527-32. 2005
    ..The lone KRAB-A and composite KRAB-AB domains from the human ZNF2 protein were over-expressed as recombinant proteins in E. coli, isolated and purified to homogeneity to investigate their structure to function relationship...
  85. ncbi Purification and characterization of kininogens from sheep plasma
    Shahid P Baba
    Department of Biochemistry, Faculty of Life Science, Aligarh Muslim University, Aligarh 202002, India
    Protein J 24:95-102. 2005
    ..40 and 2.00 nM respectively. Binding of HMWK, LMWK1 and 2 to activated papain were accompanied by pronounced changes in secondary and tertiary structure that are compatible with perturbations of environment of aromatic residues...
  86. ncbi Isolation and preliminary characterization of a 22-kDa protein with trypsin inhibitory activity from toad Bufo andrewsi skin
    Yu Zhao
    Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, People s Republic of China
    Toxicon 46:277-81. 2005
    ..The N-terminal sequence of BATI is EKDSITD, which shows no similarity with other known trypsin inhibitors...
  87. ncbi Histamine N-methyltransferase from rat kidney. Cloning, nucleotide sequence, and expression in Escherichia coli cells
    M Takemura
    Department of Pharmacology II, Osaka University Faculty of Medicine, Suita, Japan
    J Biol Chem 267:15687-91. 1992
    ..All of the inhibitors of the natural enzyme tested showed similar Ki values on both recombinant and natural enzyme...
  88. ncbi A disulfide bridge mediated by cysteine 574 is formed in the dimer of the 70-kDa heat shock protein
    Takayuki K Nemoto
    Division of Oral Molecular Biology, Department of Developmental and Reconstructive Medicine, Course of Medical and Dental Sciences, Nagasaki University Graduate School of Biomedical Sciences, 1 7 1 Sakamoto, Nagasaki 852 8588
    J Biochem 139:677-87. 2006
    ..Interestingly, the dimer formed through truncation from the C-terminus acquired a homomeric disulfide bridge at Cys574...
  89. ncbi Large complex formation of the inhibitor of caspase-activated DNase
    H Kanouchi
    Department of Biochemistry, Kawasaki Medical School, 577 Matsushima, Kurashiki, Okayama 701 0192, Japan
    Apoptosis 10:651-6. 2005
    ..When purified recombinant ICAD protein was separated by gel chromatography, the molecular weight of ICAD was detected at approximately 440 and 45 K...
  90. ncbi Purification and characterization of two low molecular weight endoglucanases produced by Penicillium occitanis mutant Pol 6
    Semia Ellouz Chaabouni
    National School of Engineers of Sfax, BP W 3038 Sfax Cedex, Tunisia
    Appl Biochem Biotechnol 125:99-112. 2005
    ..Only EG B showed xylanase activity. Furthermore, these EGs were insensitive to the action of glucose and cellobiose but were inhibited by the divalent cations Hg2+, Co2+, and Mn2+...
  91. pmc Purification, crystallization and characterization of N-acetylneuraminate lyase from Escherichia coli
    K Aisaka
    Tokyo Research Laboratories, Kyowa Hakko Kogyo Co Ltd, Japan
    Biochem J 276:541-6. 1991
    ....
  92. ncbi Purification and properties of Saccharomyces cerevisiae acetolactate synthase from recombinant Escherichia coli
    C Poulsen
    Danisco A S, Biotechnology Research Division, Copenhagen, Denmark
    Eur J Biochem 185:433-9. 1989
    ..The purified yeast acetolactate synthase was further characterized with respect to pH optimum, dependence of the substrate, pyruvate, and requirements of the cofactors, thiamin diphosphate, Mg2+, and FAD...
  93. ncbi Purification and characterization of high molecular weight forms of inhibin from bovine follicular fluid
    K Sugino
    Frontier Research Program, Institute of Physical and Chemical Research RIKEN, Saitama, Japan
    Endocrinology 130:789-96. 1992
    ..On the basis of our findings, we propose that in bovine follicular fluid, the larger 105-kDa form of inhibin is processed successively to form the lowest molecular mass form, 32 kDa inhibin, through the smaller 95- and 55-kDa forms...
  94. ncbi Production of recombinant human alpha1-microglobulin and mutant forms involved in chromophore formation
    Anna Kwasek
    Department of Physical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, ul Gronostajowa 7, 30 387 Krakow, Poland
    Protein Expr Purif 53:145-52. 2007
    ....
  95. ncbi Gluconate dehydratase from the promiscuous Entner-Doudoroff pathway in Sulfolobus solfataricus
    Henry J Lamble
    Department of Biology and Biochemistry, Centre for Extremophile Research, University of Bath, Bath BA2 7AY, UK
    FEBS Lett 576:133-6. 2004
    ..solfataricus is promiscuous for the metabolism of both glucose and galactose...
  96. ncbi dUTPase and nucleocapsid polypeptides of the Mason-Pfizer monkey virus form a fusion protein in the virion with homotrimeric organization and low catalytic efficiency
    Orsolya Barabas
    Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, POB 7, H 1518, Budapest, Hungary
    J Biol Chem 278:38803-12. 2003
    ..Binding of the hexanucleotide ACTGCC or the octanucleotide (TG)4 to NC-dUTPase modulates enzymatic function, indicating that the low catalytic activity may be compensated by adequate localization...
  97. ncbi Purification of the MutX protein of Streptococcus pneumoniae, a homologue of Escherichia coli MutT. Identification of a novel catalytic domain for nucleoside triphosphate pyrophosphohydrolase activity
    L C Bullions
    McCollum Pratt Institute, Johns Hopkins University, Baltimore, Maryland 21218
    J Biol Chem 269:12339-44. 1994
    ..coli orf17 gene (Takahagi, M., Iwasaki, H., Nakata, A., and Shinegawa, H. (1991) J. Bacteriol. 173, 5747-5753)...
  98. ncbi Molecular cloning and characterization of chondroitin polymerase from Escherichia coli strain K4
    Toshio Ninomiya
    Institute for Molecular Science of Medicine, Aichi Medical University, Yazako, Nagakute, Aichi 480 1195, Japan
    J Biol Chem 277:21567-75. 2002
    ..These results may lead to elucidation of the mechanism for chondroitin chain synthesis in both microorganisms and mammals...
  99. ncbi Complex of pregnancy-associated plasma protein-A and the proform of eosinophil major basic protein. Disulfide structure and carbohydrate attachment
    Michael T Overgaard
    Department of Molecular Biology, Science Park, University of Aarhus, Gustav Wieds Vej 10C, Denmark
    J Biol Chem 278:2106-17. 2003
    ..Furthermore, it will allow rational experimental design of functional studies aimed at understanding the formation of the PAPP-A.pro-MBP complex, as well as the inhibitory mechanism of pro-MBP...
  100. ncbi Purification and characterization of the heat shock proteins HslV and HslU that form a new ATP-dependent protease in Escherichia coli
    S J Yoo
    Department of Molecular Biology and Research Center for Cell Differentiation, College of Natural Sciences, Seoul National University, Seoul 151 742, Korea
    J Biol Chem 271:14035-40. 1996
    ..Therefore ATP appears necessary for oligomerization of HslU. Thus the HslVU protease appears to be a two-component protease in which HslV harbors the peptidase activity, while HslU provides an essential ATPase activity...
  101. ncbi Purification, characterization, antibacterial activity and N-terminal sequencing of buffalo-milk lysozyme
    Subhadra Priyadarshini
    Division of Animal Biochemistry, National Dairy Research Institute, Karnal, India
    J Dairy Res 69:419-31. 2002
    ..Antibacterial activity of buffalo-milk lysozyme was determined against 11 species of bacteria; out of seven Gram-positive bacteria tested, four were inhibited, while Gram-negative bacteria were resistant...

Research Grants62

  1. Cardiotrophin-Like Cytokine 1, a Candidate Molecule for the FSGS Factor
    Virginia J Savin; Fiscal Year: 2010
    ..These techniques include gel chromatography to determine the protein size and electrical charge, affinity chromatography to determine binding properties,..
  2. Protein Handling By Renal Proximal Tubule Epithelial Cells
    Nicholas Ferrell; Fiscal Year: 2013
    ....
  3. Using PET isotopes to identify drug targets and to diagnose infectious diseases
    Peter J Tonge; Fiscal Year: 2010
    ..This method relies on the incorporation of short-lived radioisotopes that decay to emit body-penetrating photons, and thus the reagents that are developed should also be useful for directly detecting pathogens in infected patients. ..
  4. Structure and Finction of AMPA subtype ionotropic glutamate receptors
    ALEXANDER SOBOLEVSKY; Fiscal Year: 2013
    ..abstract_text> ..
  5. Coordination of DNA replication, repair, and translesion DNA synthesis
    Mark D Sutton; Fiscal Year: 2013
    ..Furthermore, we anticipate that our results will identify critical steps in these evolutionarily conserved processes that can be targeted to control proficiency and fidelity of replication for therapeutic gain. ..
  6. Role of oligomeric alpha-synuclein in synucleinopathies
    Pamela J McLean; Fiscal Year: 2013
    ..The goals of the current application are to understand 1syn oligomer formation and how it relates to disease development in humans and to further explore factors that modify and rescue 1syn aggregation. ..
  7. Crystallization and structure determination of the mu-opioid receptor
    Roger K Sunahara; Fiscal Year: 2012
    ..cubic phase Y[unreadable]OR - YFP-[unreadable]OR fusion protein TM - transmembrane segment T4L - T4 lysozyme ICL - intracellular loop [unreadable]2AR - 22 adrenergic receptor DDM - dodecylmaltoside SEC - size exclusion chromatography ..
  8. Microfluidics for High-Throughput HDX-MS
    Julian P Whitelegge; Fiscal Year: 2013
    ..Whitelegge and C. K.-F. Shen) uniquely positioned to translate new technologies into ongoing scientific discovery at UCLA. ..
  9. Structural investigation of a GABA receptor subtype
    DEREK CLAXTON; Fiscal Year: 2013
    ..The results of this work will substantially contribute to the understanding of GABAA receptor structure and the allosteric effects of ligand binding on channel behavior. ..
  10. Structure-Function Studies of Endocannabinoid Biosynthetic Enzymes
    JOSE OLUCHA - ALVAREZ; Fiscal Year: 2013
    ..Ray Stevens'group. The knowledge on DAG lipases obtained through the work proposed in this project has the potential to become an important tool for the rational design of inhibitors against these key therapeutic targets. ..
  11. The impact of post-translational modification on SOD1 aggregation in ALS
    RACHEL REDLER; Fiscal Year: 2013
    ..Understanding of the factors that influence SOD1 aggregation can be used to develop novel therapeutic approaches to the prevention or mitigation of ALS symptoms. ..
  12. MOLECULAR STUDIES OF CHEMORECEPTION
    Gerald L Hazelbauer; Fiscal Year: 2013
    ..Overall, the proposed research will increase understanding of signaling by transmembrane receptors. Such signaling, present in many life processes, is relevant to human health and disease. ..
  13. Polypeptide Conformation and Interaction with Hsp70
    Silvia Cavagnero; Fiscal Year: 2009
    ..abstract_text> ..
  14. Harnessing Hsp104 to remodel aberrant conformers linked to Alzheimer's Disease
    MARY CUSHMAN NICK; Fiscal Year: 2012
    ..Hsp104 could ultimately be employed as a gene therapy treatment for humans with AD to efficiently disassemble amyloid aggregates and neutralize toxic oligomers. ..
  15. TAS::75 0849::TAS PURIFICATION, REFORMULATION AND PHARMACOKINETICS OF NITROSYLCO
    Joseph Bauer; Fiscal Year: 2010
    ..Purity (98%) of nitrosylcobalamin as determined by HPLC analysis. ..
  16. Using Dendrimers to Design Multivalent Therapeutic Agents
    Mary J Cloninger; Fiscal Year: 2013
    ..We also propose to perform homotypic cellular aggregation assays, chemotaxis and migration assays, mechanoelastic studies, kinetic studies on release of prodrug mimics, and studies with C. elegans. ..
  17. PROTEOGLYCAN SYNTHESIS DURING DEVELOPMENT AND AGING
    Arnold Caplan; Fiscal Year: 1999
    ..Following partial enzymatic depolymerization of chains in each population, gel chromatography will be used to obtain the hexasaccharide component of the digestion products...
  18. A Structure and Function Study: The Transport Channel of the Nuclear Pore Complex
    Richard A Wing; Fiscal Year: 2013
    ..Finally, using western blotting and mass spectrometry, I will probe for additional interactions between the adaptor layer and transport channel. ..
  19. DOMINANT WHITE (I) GENE OF CHICKENS: MODEL FOR VITILIGO
    ROGER BOWERS; Fiscal Year: 1991
    ..the effect of alpha-MSH on viability of the WL and JF melanocytes in fresh and in unchanged media; and (3) gel chromatography to distinguish the active toxic fraction in WL conditioned media followed by high performance liquid ..
  20. ACTIVE SPECIFIC IMMUNOTHERAPY IN MAN--A MURINE MODEL
    Barry Kahan; Fiscal Year: 1992
    ..The proposed studies thus proffer a molecular approach to dissect potential antigens augmenting host resistance for active specific immunotherapy...
  21. X-LINKED HYPOPHOSPHATEMIA: IGF-1 INHIBITORS
    Ralph Meyer; Fiscal Year: 1990
    ..This will permit better treatment of the human disease and improve the quality of life for patients with this common metabolic disorder...
  22. LYSOSOMAL PROTEASES OF SPERMATOZOA
    JOHN MC DONALD; Fiscal Year: 1993
    ....
  23. BIOCHEMICAL PROBES OF THE ACTIVE SITE OF AROMATASE
    ROBERT BRUEGGEMEIER; Fiscal Year: 1993
    ..microsomes is accomplished employing sodium cholate solubilization, ammonium sulfate fractionation, and gel chromatography. Two radiolabeled enzyme-activated inhibitors are being examined, and biochemical studies recently ..
  24. Synthesis and Evaluation of Nano-antioxidants for Medical Applications
    Choon Lee; Fiscal Year: 2009
    ....
  25. REPAIR OF HAIR BUNDLES IN SEA ANEMONES
    GLEN WATSON; Fiscal Year: 1999
    ..Long range plans for this project including testing the usefulness of anemone repair proteins as possible therapeutic agents for damaged hair bundles in vertebrates. ..
  26. SERUM PROTEIN BINDING THE HN AUTOANTIGEN
    JOHN KANALAS; Fiscal Year: 1993
    ....
  27. Biocompatible Dendritic Polymers : In Vivo Applications
    FRANCIS SZOKA; Fiscal Year: 2002
    ..abstract_text> ..
  28. GENE TRANSFER TO AIRWAY EPITHELIAL CELLS
    Pi Wan Cheng; Fiscal Year: 2000
    ..We propose to test the hypothesis that the liposome-receptor ligand-DNA complex by Sepharose gel chromatography and then characterize the physicochemical properties of the putative transfection complex by biochemical and ..
  29. tRNA mediated assembly in an icosahedral RNA virus
    A L Rao; Fiscal Year: 2006
    ..Collectively, the proposed studies will advance our understanding of the assembly mechanisms of icosahedral viruses that are known to cause economically important diseases in humans, animals, insects and plants. ..
  30. ETIOLOGIC FACTOR IN FOCAL GLOMERULOSCLEROSIS
    Virginia Savin; Fiscal Year: 2000
    ..Application of this information will permit early identification of patients with the most aggressive forms of the disease, and, eventually, to design specific treatment for FSGS in native kidneys and renal allografts. ..
  31. HEMATOPOIETIC CELLS AND LDL METABOLISM
    Carolyn Bigelow; Fiscal Year: 1990
    ....
  32. CHARACTERIZATION OF THE LEUKOCYTE INS (1,4,5)P3 RECEPTOR
    PETER BRADFORD; Fiscal Year: 1991
    ..Sequence data will then be derived. These studies may ultimately enable a better understanding of intracellular regulation of Ca2+ release in neutrophils...
  33. STRUCTURE AND DYNAMICS OF IMMUNE COMPLEXES
    Martin Yarmush; Fiscal Year: 1991
    ....
  34. OXIDATIVE STRESS EFFECT ON DEGRADATION DEBRIS
    John Schmitz; Fiscal Year: 1999
    ..Data will be considered significant if p less than 0.05. Benefit: Data derived from this study could provide information which would allow modifications to existing biomaterials to provide an enhanced cellular response. ..
  35. BACTERIAL METAL DETOXIFICATION--EXTRACELLULAR CHELATORS
    Andrew Gordon; Fiscal Year: 1991
    ..Human health implications of the research regard the environmental fate of potentially toxic heavy metals, and, therefore, mechanisms by which they may be incorporated into the food chain...
  36. LONG TERM BIODEGRADATION OF ELASTOMERS MATERIALS
    P Hiltner; Fiscal Year: 2001
    ..These studies will be correlated with in vivo experiments to examine the effects of antioxidants and macrophage inhibitors on biodegradation. ..
  37. LONG TERM BIODEGRADATION OF ELASTOMERS MATERIALS
    P Hiltner; Fiscal Year: 2003
    ..These studies will be correlated with in vivo experiments to examine the effects of antioxidants and macrophage inhibitors on biodegradation. ..
  38. ISOLATION/PURIFICATION MAMMALIAN PROSTACYCLIN SYNTHASE
    KRISHNAMOORTHY SANKARAN; Fiscal Year: 1992
    ..Attempts will be made to use the monoclonal antibodies developed in a large scale, single step purification procedure. The purified enzyme, the antibodies and the nucleic acid probes will be marketed Oxford Biomedical Research...
  39. LIGAND BINDING DOMAINS OF TGF BETA RECEPTOR
    ANDRZEJ KREZEL; Fiscal Year: 2000
    ..The long range goal of this research is to discover the principles that determine locations of binding sites and specificities of new receptors, for which the direct experimental approach to the ligand-receptor complex is not feasible. ..
  40. POST-TRANSCRIPTIONAL CONTROL OF PROINSULIN SYNTHESIS
    JOSE CIMADEVILLA; Fiscal Year: 1980
    ..Antibodies against the isolated repressor will be obtained from immunized rabbits. Finally, the inactive repressor will be purified and the mechanism responsible for its activation will be investigated...
  41. APOLIPOPROTEIN-R MUTATION & PLASMA LIPID LEVELS IN PICS
    JAN RAPACZ; Fiscal Year: 1990
    ..Preparative ultracentrifugation, delipidation, gel affinity and gel chromatography, immunochemical and immunodiffusion techniques, native, SDS and 2D PAGE, will be employed to separate, ..
  42. Protein Self-Assembly in Model Microorganisms
    James Hu; Fiscal Year: 2004
    ..cerevisiae and E. coli) and important human pathogens (M. tuberculosis and A. fumigatus), and new drug targets based on protein-protein interactions. ..
  43. CANCER-INDUCED COAGULATION--MONOCYTES/HYALURONIC ACID
    Maria McGee; Fiscal Year: 1992
    ..5x10Da) will be purified from specific-hyaluronidase digests using polyacrylamide gel chromatography. These preparations will be used to characterize the nature of the interaction between HA and coagulation ..
  44. INITIATORS AND MEDIATORS IN PERIODONTAL DISEASE
    EDWARD MONTGOMERY; Fiscal Year: 1980
    ..These studies will provide necessary information for establishing the most effective means of preventing and/or treating periodontal disease. ..
  45. NAD KINASE IN LENS TISSUE
    EUGENE MC GUINNESS; Fiscal Year: 1993
    ..Purification/fractionation will be carried out using established gel chromatography protocols then as needed, ion-exchange and affinity chromatography...
  46. Novel Controlled Delivery Systems for Contraceptives
    Jagdish Singh; Fiscal Year: 2005
    ..Development of such a novel therapeutic system is critical for successful treatment of hormone-related diseases and improvement in the patients' quality-of-life. ..
  47. Proteomics of DEP-induced Oxidative Stress
    Andre Nel; Fiscal Year: 2006
    ..as well as aromatic and polar chemical groups prepared from these particles and fractionated by silica gel chromatography. The investigators hvpothesize that redox cycling polycyclic aromatic hydrocarbons and their oxidized ..
  48. The Role of ApoE: ABeta Interactions in Intraneuronal ABeta Accumulation
    LISA JUNGBAUER; Fiscal Year: 2009
    ....
  49. STUDY OF HUMAN MULTIPLE MYELOMA CELL ANTIGENS
    Malcolm MacKenzie; Fiscal Year: 1980
    ..Antigens will be isolated and characterized by means of potassium chloride extraction gel chromatography, ion exchange chromatography and polyacrylamide gel electrophoresis...
  50. PROTEIN GLYCATION--STRUCTURE AND STABILITY OF PRODUCTS
    Ajay Pande; Fiscal Year: 1993
    ..Labelled (13C and 2H) sugars will be used in I(b) above, to prepare advanced glycation products, which will be examined by high resolution and solid-state nuclear magnetic resonance spectroscopy and vibrational spectroscopy...
  51. Investigations into the structure, function, and activity of E2-25K
    Pamela Twigg; Fiscal Year: 2009
    ..Because E2-25K is implicated in the development of some neurodegenerative diseases, it is an interesting target for drug therapy. This aim of this research is to understand how this protein functions in the cell. ..
  52. IN-VIVO EVALUATION OF SURFACE-INDUCED THROMBOGENESIS
    Stuart Cooper; Fiscal Year: 1992
    ..These studies will lead to a better understanding of the mechanisms involved in artificial surface-induced thrombogenesis and will aid in the design of biocompatible polymeric materials...
  53. ALTRUINS, NATURAL ANTIBIOTICS OF THE MALE GENITAL TRACT
    Robert Lehrer; Fiscal Year: 2001
    ..S. are sexually transmitted. According to CDC estimates, STDs other than AIDS add 10 billion dollars to the nation's annual health care I costs. The proposed studies could lead to novel approaches for STD prophylaxis. ..
  54. Decorin Deficiency in Hutchinson-Gilford Progeria
    Joan Lemire; Fiscal Year: 2004
    ....
  55. CHARACTERIZATION OF FETOPLACENTAL PTH-LIKE FACTORS
    Lewis Rubin; Fiscal Year: 1990
    ..I will examine: size, by means of bioactivity profiles of umbilical venous plasma subjected to gel chromatography; immunoreactivity, by investigating neutralization of bioactivity with different regionspecific PTH and PTH-..
  56. EMBRYONIC CELL ASSOCIATION & DEVELOPMENT
    David Yang; Fiscal Year: 1980
    ..The three activities have been separated by gel chromatography, extraction and other preliminary purification methods...
  57. COUPLING MECHANISMS--SITE-SPECIFIC PROTEIN-DNA ASSEMBLY
    DONALD SENEAR; Fiscal Year: 1993
    ..The energetic properties will be correlated with the known structures of the interacting macromolecules to delineate the roles of individual structural elements in generating the forces...
  58. RENAL WATER AND ION TRANSPORT: ROLE OF PROTEINS
    IRWIN SINGER; Fiscal Year: 1980
    ..resistance, osmotic water flow, isotope flux, radiotracer- and fluorescence-labelling of proteins, gel chromatography, acrylamide gel electrophoresis and autoradiography...
  59. PLASMA SOMATOMEDIN METABOLISM AND ACTIONS
    Raymond Hintz; Fiscal Year: 1991
    ..the IGF's The studies will utilize purified and synthetic peptides, high performance liquid chromatography, gel chromatography, ion exchange chromatography, gel electrophoresis, cDNA and mRNS probes, and autoradiography...
  60. PURIFICATION OF A FIBRINOLYSIS ENHANCING PROTEIN
    Mark Triscott; Fiscal Year: 1992
    ..We propose raising a polyclonal antibody against the protein to use in the development of immunoassays for the measurement of the protein in plasma...
  61. A NEW CLASS OF DRUG CARRIERS FOR TREATMENT OF MS
    KENNETH KOSAK; Fiscal Year: 2003
    ..The drug-loaded carriers will be purified by gel chromatography. The carrying capacity, stability, release activity, and T cell inhibition in vitro of each drug-loaded ..
  62. Structure determination of immunodeficiency virus Env spike
    Robert Pejchal; Fiscal Year: 2009
    ..An HIV vaccine offers the best hope for controlling the AIDS epidemic, which, despite decades of research, shows no sign of abating. ..