mass fragmentography

Summary

Summary: A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.

Top Publications

  1. pmc Differential metabolic networks unravel the effects of silent plant phenotypes
    Wolfram Weckwerth
    Department of Molecular Physiology, Max Planck Institute of Molecular Plant Physiology, 14424 Potsdam, Germany
    Proc Natl Acad Sci U S A 101:7809-14. 2004
  2. pmc Integration of metabolite with transcript and enzyme activity profiling during diurnal cycles in Arabidopsis rosettes
    Yves Gibon
    Max Planck Institute of Molecular Plant Physiology, Science Park Golm, Am Muehlenberg, D 14476 Potsdam Golm, Germany
    Genome Biol 7:R76. 2006
  3. ncbi Method validation for measurement of hair nicotine level in nonsmokers
    Sung Roul Kim
    The Institute for Global Tobacco Control, Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA
    Biomed Chromatogr 23:273-9. 2009
  4. ncbi MET-IDEA: data extraction tool for mass spectrometry-based metabolomics
    Corey D Broeckling
    Plant Biology Division, Samuel Roberts Noble Foundation, P O Box 2180, Ardmore, Oklahoma 73401, USA
    Anal Chem 78:4334-41. 2006
  5. ncbi A strategy for identifying differences in large series of metabolomic samples analyzed by GC/MS
    Par Jonsson
    Research Group for Chemometrics, Organic Chemistry, Department of Chemistry, Umea University, SE 901 87 Umea, Sweden
    Anal Chem 76:1738-45. 2004
  6. pmc Fluoxetine-elicited changes in brain neurosteroid content measured by negative ion mass fragmentography
    D P Uzunov
    Department of Psychiatry, University of Illinois at Chicago 60612, USA
    Proc Natl Acad Sci U S A 93:12599-604. 1996
  7. ncbi MetaboliteDetector: comprehensive analysis tool for targeted and nontargeted GC/MS based metabolome analysis
    Karsten Hiller
    Department of Bioinformatics and Biochemistry, Technische Universitat Braunschweig, Langer Kamp 19b, D 38106 Braunschweig, Germany
    Anal Chem 81:3429-39. 2009
  8. ncbi Systematic identification of conserved metabolites in GC/MS data for metabolomics and biomarker discovery
    Mark P Styczynski
    Department of Chemical Engineering, Massachusetts Institute of Technology, Room 56 469c, Cambridge, Massachusetts 02139, USA
    Anal Chem 79:966-73. 2007
  9. ncbi GMD@CSB.DB: the Golm Metabolome Database
    Joachim Kopka
    Max Planck Institute of Molecular Plant Physiology, Am Muhlenberg 1, 14476 Golm, Germany
    Bioinformatics 21:1635-8. 2005
  10. ncbi Putative chemical signals about sex, individuality, and genetic background in the preputial gland and urine of the house mouse (Mus musculus)
    Jian Xu Zhang
    State Key Laboratory of Integrated Management of Pest Insects and Rodents in Agriculture, Institute of Zoology, Chinese Academy of Sciences, 25 Beisihuanxi Road, Haidian District, Beijing 100080, China
    Chem Senses 32:293-303. 2007

Detail Information

Publications327 found, 100 shown here

  1. pmc Differential metabolic networks unravel the effects of silent plant phenotypes
    Wolfram Weckwerth
    Department of Molecular Physiology, Max Planck Institute of Molecular Plant Physiology, 14424 Potsdam, Germany
    Proc Natl Acad Sci U S A 101:7809-14. 2004
    ..Metabolic alterations could be assigned to carbohydrate and amino acid metabolism even if no difference in average metabolite levels was found...
  2. pmc Integration of metabolite with transcript and enzyme activity profiling during diurnal cycles in Arabidopsis rosettes
    Yves Gibon
    Max Planck Institute of Molecular Plant Physiology, Science Park Golm, Am Muehlenberg, D 14476 Potsdam Golm, Germany
    Genome Biol 7:R76. 2006
    ..This indicates that enzyme activities change slowly, integrating the changes in transcript levels over several diurnal cycles...
  3. ncbi Method validation for measurement of hair nicotine level in nonsmokers
    Sung Roul Kim
    The Institute for Global Tobacco Control, Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA
    Biomed Chromatogr 23:273-9. 2009
    ..In the field study (n = 2400), a positive association was evident between hair nicotine concentrations from nonsmokers and higher numbers of cigarettes smoked per day in a household...
  4. ncbi MET-IDEA: data extraction tool for mass spectrometry-based metabolomics
    Corey D Broeckling
    Plant Biology Division, Samuel Roberts Noble Foundation, P O Box 2180, Ardmore, Oklahoma 73401, USA
    Anal Chem 78:4334-41. 2006
    ..The results indicate that MET-IDEA is capable of rapidly extracting semiquantitative data from raw data files, which allows for more rapid biological insight. MET-IDEA is freely available to academic users upon request...
  5. ncbi A strategy for identifying differences in large series of metabolomic samples analyzed by GC/MS
    Par Jonsson
    Research Group for Chemometrics, Organic Chemistry, Department of Chemistry, Umea University, SE 901 87 Umea, Sweden
    Anal Chem 76:1738-45. 2004
    ..We suggest that this method can be used for rapid comparison of large sets of GC/MS data, thereby applying time-consuming deconvolution only to parts of the chromatograms that contribute to explain the differences between the samples...
  6. pmc Fluoxetine-elicited changes in brain neurosteroid content measured by negative ion mass fragmentography
    D P Uzunov
    Department of Psychiatry, University of Illinois at Chicago 60612, USA
    Proc Natl Acad Sci U S A 93:12599-604. 1996
    ..A fluoxetine stimulation of brain 3 alpha, 5 alpha-TH PROG biosynthesis might be operative in the anxiolytic and antidysphoric actions of this drug...
  7. ncbi MetaboliteDetector: comprehensive analysis tool for targeted and nontargeted GC/MS based metabolome analysis
    Karsten Hiller
    Department of Bioinformatics and Biochemistry, Technische Universitat Braunschweig, Langer Kamp 19b, D 38106 Braunschweig, Germany
    Anal Chem 81:3429-39. 2009
    ..The features of MetaboliteDetector are demonstrated by the analysis of two experimental metabolomics data sets. MetaboliteDetector is freely available under the GNU public license (GPL) at http://metabolitedetector.tu-bs.de...
  8. ncbi Systematic identification of conserved metabolites in GC/MS data for metabolomics and biomarker discovery
    Mark P Styczynski
    Department of Chemical Engineering, Massachusetts Institute of Technology, Room 56 469c, Cambridge, Massachusetts 02139, USA
    Anal Chem 79:966-73. 2007
    ..By systematically cataloguing conserved metabolite peaks prior to data analysis methods, our approach broadens the scope of metabolomics and facilitates biomarker discovery...
  9. ncbi GMD@CSB.DB: the Golm Metabolome Database
    Joachim Kopka
    Max Planck Institute of Molecular Plant Physiology, Am Muhlenberg 1, 14476 Golm, Germany
    Bioinformatics 21:1635-8. 2005
    ..AVAILABILITY: http://csbdb.mpimp-golm.mpg.de/gmd.html CONTACT: Steinhauser@mpimp-golm.mpg.de SUPPLEMENTARY INFORMATION: http://csbdb.mpimp-golm.mpg.de/..
  10. ncbi Putative chemical signals about sex, individuality, and genetic background in the preputial gland and urine of the house mouse (Mus musculus)
    Jian Xu Zhang
    State Key Laboratory of Integrated Management of Pest Insects and Rodents in Agriculture, Institute of Zoology, Chinese Academy of Sciences, 25 Beisihuanxi Road, Haidian District, Beijing 100080, China
    Chem Senses 32:293-303. 2007
    ..Hence, these 2 compounds might code for information about their genetic background...
  11. pmc Metabolic profiling of transgenic tomato plants overexpressing hexokinase reveals that the influence of hexose phosphorylation diminishes during fruit development
    Ute Roessner-Tunali
    Department of Lothar Willmitzer, Max Planck Institut fur Molekulare Pflanzenphysiologie, Am Muhlenberg 1, 14476 Golm, Germany
    Plant Physiol 133:84-99. 2003
    ..In summary, the data presented in this paper demonstrate that the influence of hexose phosphorylation diminishes during fruit development and highlights the importance of greater temporal resolution of metabolism...
  12. ncbi Determination of volatile organic compounds in exhaled breath of patients with lung cancer using solid phase microextraction and gas chromatography mass spectrometry
    Magdalena Ligor
    Department of Anaesthesiology and Critical Care Medicine, Innsbruck Medical University, Innsbruck, Austria
    Clin Chem Lab Med 47:550-60. 2009
    ..This might lead to a future diagnostic test for lung cancer...
  13. pmc Novel modification of lipid A of Francisella tularensis
    Nancy J Phillips
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California, USA
    Infect Immun 72:5340-8. 2004
    ..tularensis containing a phosphate-linked galactosamine could potentially influence its intracellular survival by conferring resistance to antimicrobial peptides...
  14. ncbi Variation in volatile organic compounds in the breath of normal humans
    M Phillips
    Menssana Research, Fort Lee, NJ 07024, USA
    J Chromatogr B Biomed Sci Appl 729:75-88. 1999
    ..This study confirmed previous reports of wide inter-individual variations. Two new findings were the comparatively small variation in total number of breath VOCs, and the presence of a 'common core' of breath VOCs in all subjects...
  15. ncbi Quality control for plant metabolomics: reporting MSI-compliant studies
    Oliver Fiehn
    Davis Genome Center, University of California, Davis, CA 95616, USA
    Plant J 53:691-704. 2008
    ..Standardized data processing methods are proposed for consistent data storage and dissemination via databases...
  16. pmc Lotus japonicus metabolic profiling. Development of gas chromatography-mass spectrometry resources for the study of plant-microbe interactions
    Guilhem G Desbrosses
    Max Planck Institute of Molecular Plant Physiology, 14476 Golm, Germany
    Plant Physiol 137:1302-18. 2005
    ..japonicus and Mesorhizobium loti, should lead to a better understanding of nodule metabolism that underpins SNF...
  17. ncbi Fresh organically grown ginger (Zingiber officinale): composition and effects on LPS-induced PGE2 production
    Shivanand D Jolad
    Arizona Center for Phytomedicine Research, College of Pharmacy, University of Arizona, Tucson, AZ 85721, USA
    Phytochemistry 65:1937-54. 2004
    ..Most of the fractions containing gingerols and/or gingerol derivatives showed excellent inhibition of LPS-induced PGE2 production...
  18. ncbi Desaturases from the spotted fireworm moth (Choristoneura parallela) shed light on the evolutionary origins of novel moth sex pheromone desaturases
    Weitian Liu
    Department of Entomology, Cornell University, Geneva, NY 14456, USA
    Gene 342:303-11. 2004
    ....
  19. ncbi Food wrapping with the postpharyngeal gland secretion by females of the European beewolf Philanthus triangulum
    Gudrun Herzner
    Department of Animal Ecology and Tropical Biology, University of Wurzburg, Biozentrum, Am Hubland, D 97074, Wurzburg, Germany
    J Chem Ecol 33:849-59. 2007
    ..We discuss our findings with regard to other possible mechanisms of the postpharyngeal gland secretion against fungal growth...
  20. pmc Complete release of (5'S)-8,5'-cyclo-2'-deoxyadenosine from dinucleotides, oligodeoxynucleotides and DNA, and direct comparison of its levels in cellular DNA with other oxidatively induced DNA lesions
    Pawel Jaruga
    Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County, MD 22777, USA
    Nucleic Acids Res 32:e87. 2004
    ..We conclude that (5'S)-cdA can be completely released from DNA by enzymic hydrolysis, and the level of (5'S)-cdA in tissue DNA is comparable to those of other oxidatively induced DNA lesions...
  21. ncbi Metabolomic technologies and their application to the study of plants and plant-host interactions
    J William Allwood
    School of Chemistry, Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess Street, Manchester M1 7DN, UK
    Physiol Plant 132:117-35. 2008
    ....
  22. ncbi MetaQuant: a tool for the automatic quantification of GC/MS-based metabolome data
    Boyke Bunk
    Institute of Microbiology, Technical University of Braunschweig, Spielmannstr 7 D 38106 Braunschweig, Germany
    Bioinformatics 22:2962-5. 2006
    ..Finally, MetaQuant is able to import GC/MS data in the common NetCDF format and to export the results of the quantification into Systems Biology Markup Language (SBML), Comma Separated Values (CSV) or Microsoft Excel (XLS) format...
  23. ncbi Gas chromatography mass spectrometry-based metabolite profiling in plants
    Jan Lisec
    Max Planck Institut fur Molekulare Pflanzenphysiologie, Am Muhlenberg 1, 14476 Potsdam Golm, Germany
    Nat Protoc 1:387-96. 2006
    ..Using the protocol described here facilitates routine determination of the relative levels of 300-500 analytes of polar and nonpolar extracts in approximately 400 experimental samples per week per machine...
  24. ncbi Global metabolic profiling of Escherichia coli cultures: an evaluation of methods for quenching and extraction of intracellular metabolites
    Catherine L Winder
    School of Chemistry, The University of Manchester, 131 Princess Street, Manchester, M1 7DN, United Kingdom
    Anal Chem 80:2939-48. 2008
    ....
  25. ncbi Mass spectrometry-based metabolic profiling reveals different metabolite patterns in invasive ovarian carcinomas and ovarian borderline tumors
    Carsten Denkert
    Institute of Pathology and Department of Gynecology and Obstetrics, Charite University Hospital, Berlin, Germany
    Cancer Res 66:10795-804. 2006
    ..We conclude that metabolomics is a promising high-throughput, automated approach in addition to functional genomics and proteomics for analyses of molecular changes in malignant tumors...
  26. pmc Metabolite profiling of Chlamydomonas reinhardtii under nutrient deprivation
    Christian Bölling
    Max Planck Institute of Molecular Plant Physiology, 14424 Potsdam, Germany
    Plant Physiol 139:1995-2005. 2005
    ....
  27. ncbi Potential of metabolomics as a functional genomics tool
    Raoul J Bino
    Plant Physiology Department, Wageningen University, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands
    Trends Plant Sci 9:418-25. 2004
  28. ncbi The soluble and membrane-bound transhydrogenases UdhA and PntAB have divergent functions in NADPH metabolism of Escherichia coli
    Uwe Sauer
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    J Biol Chem 279:6613-9. 2004
    ..metabolism with an extraordinary flexibility to cope with varying catabolic and anabolic demands, which raises two general questions: why do only a few bacteria contain both isoforms, and how do other organisms manage NADPH metabolism?..
  29. ncbi TagFinder for the quantitative analysis of gas chromatography--mass spectrometry (GC-MS)-based metabolite profiling experiments
    Alexander Luedemann
    Department Prof L Willmitzer, Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, D 14476 Potsdam Golm, Germany
    Bioinformatics 24:732-7. 2008
    ..So far no software tool supports the non-targeted and user-independent quality assessment of the data matrices prior to statistical analysis. TagFinder may fill this gap...
  30. pmc A novel approach for nontargeted data analysis for metabolomics. Large-scale profiling of tomato fruit volatiles
    Yury Tikunov
    Centre for BioSystems Genomics, 6700 AB Wageningen, The Netherlands
    Plant Physiol 139:1125-37. 2005
    ..The approach chosen will further enhance the comprehensiveness of GC-MS-based metabolomics approaches and will therefore prove a useful addition to nontargeted functional genomics research...
  31. ncbi Identification and quantification of 8,5'-cyclo-2'-deoxy-adenosine in DNA by liquid chromatography/ mass spectrometry
    M Dizdaroglu
    Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899 8311, USA
    Free Radic Biol Med 30:774-84. 2001
    ..The measurement of 8,5'-cyclo-2'-deoxyadenosine by liquid chromatography/mass spectrometry may contribute to the understanding of its biological properties and its role in diseases with defective nucleotide-excision repair...
  32. pmc Antioxidant-induced changes in oxidized DNA
    Donald C Malins
    Biochemical Oncology Program and Tumor Immunology Program, Pacific Northwest Research Institute, 720 Broadway, Seattle, WA 98122, USA
    Proc Natl Acad Sci U S A 99:5937-41. 2002
    ..These benefits could be offset by the other structural changes found after NAC exposure, which may affect the fidelity of DNA synthesis...
  33. ncbi Analysis of the sex pheromone extract of individual male Lutzomyia longipalpis sandflies from six regions in Brazil
    J G C Hamilton
    Chemical Ecology Medical Entomology Group, Centre for Applied Entomology and Parasitology, Institute of Science and Technology in Medicine, Keele University, Staffordshire, UK
    Med Vet Entomol 19:480-8. 2005
    ..Evidence of a fifth chemotype in Brazil is also presented...
  34. ncbi Evaluation of androstenedione as an androgenic component of river water downstream of a pulp and paper mill effluent
    Eth J Durhan
    US Environmental Protection Agency, National Health and Environmental Effects Laboratory, Duluth, Minnesota 55804, USA
    Environ Toxicol Chem 21:1973-6. 2002
    ..We conclude that, although androstenedione was present at easily detectable concentrations in the river water (> 100 ng/L), this compound is not associated with androgenic activity of water from the site...
  35. ncbi Maternal urine and serum steroid measurements to identify steroid sulfatase deficiency (STSD) in second trimester pregnancies
    Josep Marcos
    Children s Hospital Oakland Research Institute, Oakland, CA, USA
    Prenat Diagn 29:771-80. 2009
    ..To document the performance of second trimester maternal urine and serum steroid measurements for detecting fetal steroid sulfatase deficiency (STSD)...
  36. ncbi Potential chemosignals in the anogenital gland secretion of giant pandas, Ailuropoda melanoleuca, associated with sex and individual identity
    Jian Xu Zhang
    State Key Laboratory of Integrated Management of Pest Insects and Rodents in Agriculture, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100080, China
    J Chem Ecol 34:398-407. 2008
    ..Although putative sex pheromones such as squalene and erucic acid should be assessed further by bioassay, our study suggests that synthetic chemosignals might be useful in modulating the behavior and physiology of giant pandas...
  37. pmc Metabolic profiling allows comprehensive phenotyping of genetically or environmentally modified plant systems
    U Roessner
    , , 14476 Golm, Germany
    Plant Cell 13:11-29. 2001
    ..In summary, these data demonstrate the use of metabolic profiling in conjunction with data-mining tools as a technique for the comprehensive characterization of a plant genotype...
  38. ncbi Direct and indirect defences induced by piercing-sucking and chewing herbivores in Medicago truncatula
    Margit Leitner
    Max Planck Institut für Chemische Okologie, Abteilung Bioorganische Chemie, Hans Knöll Str 8, D 07745 Jena, Germany
    New Phytol 167:597-606. 2005
    ..In contrast, no divergence in direct defences was observed; furthermore, the traits investigated exhibited striking similarities to reactions known to occur upon pathogen attack...
  39. ncbi Design of experiments: an efficient strategy to identify factors influencing extraction and derivatization of Arabidopsis thaliana samples in metabolomic studies with gas chromatography/mass spectrometry
    Jonas Gullberg
    Umea Plant Science Center, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE 901 87 Umea, Sweden
    Anal Biochem 331:283-95. 2004
    ..Further, the reproducibility of the analysis of 66 endogenous compounds was investigated, and it was shown that both hydrophilic and lipophilic compounds were detected with high reproducibility...
  40. ncbi Extraction and GC/MS analysis of the human blood plasma metabolome
    Jiye A
    Department of Medical Biosciences, Clinical Chemistry, Umea University, SE 90185, Umea, Sweden, and Department of Clinical Pharmacology, The First Affiliated Hospital of Nanjing Medical University, 210029, Nanjing, China
    Anal Chem 77:8086-94. 2005
    ..1 and 1 microM. The results suggest that the method could be usefully integrated into metabolomic studies for various purposes, e.g., for identifying biological markers related to diseases...
  41. pmc New components of the honey bee (Apis mellifera L.) queen retinue pheromone
    Christopher I Keeling
    Department of Chemistry, Simon Fraser University, 8888 University Drive, Burnaby, BC, Canada V5A 1S6
    Proc Natl Acad Sci U S A 100:4486-91. 2003
    ..The queen therefore produces a synergistic, multiglandular pheromone blend of at least nine compounds for retinue attraction, the most complex pheromone blend known for inducing a single behavior in any organism...
  42. pmc Fertility in four regions spanning large contrasts in serum levels of widespread persistent organochlorines: a cross-sectional study
    Gunnar Toft
    Department of Occupational Medicine, Aarhus University Hospital, Noerrebrogade 44, Build, 2C, DK 8000 Aarhus C, Denmark
    Environ Health 4:26. 2005
    ..Persistent organochlorine pollutants (POPs) may interfere with reproductive function but direct evidence in humans is very limited...
  43. ncbi High-throughput data analysis for detecting and identifying differences between samples in GC/MS-based metabolomic analyses
    Par Jonsson
    Research Group for Chemometrics, Organic Chemistry, Department of Chemistry, Umea University, SE 901 87 Umea, Sweden
    Anal Chem 77:5635-42. 2005
    ..The processing of 70 samples took similar time to that of the GC/TOFMS analyses of the samples. The strategy has been validated using two different sets of samples: a complex mixture of standard compounds and Arabidopsis samples...
  44. ncbi Analytical methods of biological monitoring for exposure to pesticides: recent update
    Maria G Margariti
    Departments of Medicine, Centre of Toxicology Science and Research, School of Health Sciences, University of Crete, Crete, Greece
    Ther Drug Monit 29:150-63. 2007
    ....
  45. pmc Characterization of Z/E11- and Z9-desaturases from the obliquebanded leafroller moth, Choristoneura rosaceana
    Guixia Hao
    Department of Entomology, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456, USA
    J Insect Sci 2:26. 2002
    ..A metabolic Z9-desaturase also was cloned from fat body of this species, and proved to be in the class that produces more Z9-16:Acid than Z9-18:Acid...
  46. ncbi Development and characterization of liposomal disodium ascorbyl phytostanyl phosphates (FM-VP4)
    Agatha W K Ng
    Division of Pharmaceutics and Biopharmaceutics, Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, British Columbia, Canada
    Drug Dev Ind Pharm 30:739-58. 2004
    ..This project proposed a framework for such delivery and paves the way for further investigation into how FM-VP4 works in vivo and in vitro...
  47. ncbi Stir bar sorptive extraction: a new quantitative and comprehensive sampling technique for determination of chemical signal profiles from biological media
    Helena A Soini
    Department of Chemistry, Institute for Pheromone Research Indiana University, Bloomington, Indiana 47405, USA
    J Chem Ecol 31:377-92. 2005
    ..Its analytical merits are compared with those of the previously established purge-and-trap (dynamic headspace) technique...
  48. ncbi Volatile organic compounds in breath as markers of lung cancer: a cross-sectional study
    M Phillips
    Menssana Research Inc, Fort Lee, New Jersey, USA
    Lancet 353:1930-3. 1999
    ..Many volatile organic compounds (VOCs), principally alkanes and benzene derivatives, have been identified in breath from patients with lung cancer. We investigated whether a combination of VOCs could identify such patients...
  49. ncbi Plant metabolomics reveals conserved and divergent metabolic responses to salinity
    Diego H Sanchez
    Max Planck Institute for Molecular Plant Physiology, Wissenschaftspark Golm, Am Muehlenberg 1, Potsdam Golm, 14476, Germany
    Physiol Plant 132:209-19. 2008
    ..We present evidence for conserved and divergent metabolic responses among these three species and conclude that a change in the balance between amino acids and organic acids may be a conserved metabolic response of plants to salt stress...
  50. ncbi A systems biology investigation of the MEP/terpenoid and shikimate/phenylpropanoid pathways points to multiple levels of metabolic control in sweet basil glandular trichomes
    Zhengzhi Xie
    Department of Plant Sciences and BIO5 Institute, University of Arizona, Tucson, AZ 85721, USA
    Plant J 54:349-61. 2008
    ..Additionally, precursors for different classes of terpenoids, including mono- and sesquiterpenoids, appear to be almost exclusively supplied by the MEP pathway, and not the mevalonate pathway, in basil glandular trichomes...
  51. ncbi Diagnosing lung cancer in exhaled breath using gold nanoparticles
    Gang Peng
    Department of Chemical Engineering, Technion Israel Institute of Technology, Haifa 32000, Israel
    Nat Nanotechnol 4:669-73. 2009
    ..Our results show that sensors based on gold nanoparticles could form the basis of an inexpensive and non-invasive diagnostic tool for lung cancer...
  52. pmc Gene expression and metabolism in tomato fruit surface tissues
    Shira Mintz-Oron
    Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
    Plant Physiol 147:823-51. 2008
    ..Peel-associated genes identified in our study, together with comparative analysis of genes enriched in surface tissues of various other plant species, establish a springboard for future investigations of plant surface biology...
  53. ncbi Free radical-induced damage to DNA: mechanisms and measurement
    Miral Dizdaroglu
    Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899 8311, USA
    Free Radic Biol Med 32:1102-15. 2002
    ..This article reviews mechanistic aspects of oxidative damage to DNA and recent developments in the measurement of this type of damage using chromatographic and mass spectrometric techniques...
  54. ncbi NMR study of virenose and dihydrohydroxystreptose isolated from Coxiella burnetii phase I lipopolysaccharide
    R Toman
    Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic
    Carbohydr Res 306:291-6. 1998
    ..Radziejewska-Lebrecht, and H. Mayer, Eur. J. Biochem., 148 (1985) 455-461] to be present in LPS I in a furanose form, and it appears that a furanose to pyranose tautomerization took place in the course of the isolation procedure...
  55. pmc Analysis of the compartmentation of glycolytic intermediates, nucleotides, sugars, organic acids, amino acids, and sugar alcohols in potato tubers using a nonaqueous fractionation method
    E M Farré
    Max Planck Institut fur Molekulare Pflanzenphysiologie, Am Muhlenberg 1, 14476 Golm, Germany
    Plant Physiol 127:685-700. 2001
    ..However, due to the low plastidic pyrophosphate concentration, the reaction catalyzed by ADP-glucose pyrophosphorylase was estimated to be far removed from equilibrium...
  56. ncbi Real-time monitoring of herbivore induced volatile emissions in the field
    Andrea Schaub
    Ionicon Analytik GmbH, Technikerstrasse 21a, 6020 Innsbruck, Austria
    Physiol Plant 138:123-33. 2010
    ..However, there was a clear effect on SQT measurements. Elucidation of emission patterns in real-time is dependent upon the dynamics of cuvettes at different temperatures...
  57. ncbi Analysis of polybrominated diphenyl ethers in Swedish human milk. A time-related trend study, 1972-1997
    D Meironyté
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    J Toxicol Environ Health A 58:329-41. 1999
    ..In total, eight PBDE congeners were identified in the milk. The sum of the concentrations of BDE congeners in human milk increased from 0.07 to 4.02 ng/g lipids during the 25-yr period studied...
  58. ncbi Identification of major histocompatibility complex-regulated body odorants by statistical analysis of a comparative gas chromatography/mass spectrometry experiment
    Alan Willse
    Pacific Northwest National Laboratory, 902 Battelle Boulevard, Richland, Washington 99354, USA
    Anal Chem 77:2348-61. 2005
    ..Several dozen MHC-regulated compounds are found, including two known mouse pheromones, 2,5-dimethylpyrazine and 2-sec-butyl-4,5-dihydrothiazole...
  59. pmc Systems rebalancing of metabolism in response to sulfur deprivation, as revealed by metabolome analysis of Arabidopsis plants
    Victoria J Nikiforova
    Max Planck Institute of Molecular Plant Physiology, Golm 14476, Germany
    Plant Physiol 138:304-18. 2005
    ..These responses may be assembled into a global scheme of metabolic regulation induced by sulfur nutritional stress, which optimizes resources for seed production...
  60. ncbi Metabolite profiling for plant functional genomics
    O Fiehn
    Max Planck Institute of Molecular Plant Physiology, 14424 Potsdam, Germany
    Nat Biotechnol 18:1157-61. 2000
    ..These results demonstrate the use of metabolite profiling as a tool to significantly extend and enhance the power of existing functional genomics approaches...
  61. ncbi Identification of hydroxylated metabolites in 2,2',4,4'-tetrabromodiphenyl ether exposed rats
    Göran Marsh
    Department of Environmental Chemistry, Stockholm University, SE 106 91 Stockholm, Sweden
    Chemosphere 63:690-7. 2006
    ..Two of the identified OH-PBDE metabolites have identical structures as natural products, which previously have been isolated from marine sponges and an ascidian...
  62. ncbi Current challenges and developments in GC-MS based metabolite profiling technology
    Joachim Kopka
    Max Planck Institute of Molecular Plant Physiology, Department Molecular Plant Physiology Prof L Willmitzer, Golm, Germany
    J Biotechnol 124:312-22. 2006
    ..csbdb.mpimp-golm.mpg.de/gmd.html)...
  63. ncbi Multi-residue screening of a minimum package of anabolic steroids in urine with GC-MS
    P R Kootstra
    RIVM, National Institute of Public Health and the Environment, Laboratory for Food and Residue Analyses, European Union Community Reference Laboratory, P O Box 1, 3720 BA Bilthoven, The Netherlands
    Anal Chim Acta 586:82-92. 2007
    ..Results of validation experiments are presented. The method is flexible and due to the non-specific sample clean-up more and new anabolic compounds can be easily added in order to new monitoring requirements...
  64. pmc An iterative block-shifting approach to retention time alignment that preserves the shape and area of gas chromatography-mass spectrometry peaks
    Minho Chae
    UALR UAMS Joint Graduate Program in Bioinformatics, University of Arkansas at Little Rock, Little Rock, AR 72204, USA
    BMC Bioinformatics 9:S15. 2008
    ....
  65. pmc The absence of ALTERNATIVE OXIDASE1a in Arabidopsis results in acute sensitivity to combined light and drought stress
    Estelle Giraud
    Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia
    Plant Physiol 147:595-610. 2008
    ....
  66. ncbi Microbial metabolomics with gas chromatography/mass spectrometry
    Maud M Koek
    Analytical Science Department, TNO Quality of Life, Utrechtseweg 48, 3704 HE Zeist, The Netherlands
    Anal Chem 78:1272-81. 2006
    ..The suitability of the method was demonstrated by analysis of E. coli samples harvested at different growth phases...
  67. ncbi A flux model of glycolysis and the oxidative pentosephosphate pathway in developing Brassica napus embryos
    Jorg Schwender
    Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824, USA
    J Biol Chem 278:29442-53. 2003
    ..Therefore, the reductant produced by the OPPP accounts for at most 44% of the NADPH and 22% of total reductant needed for fatty acid synthesis...
  68. ncbi Metabolomic analysis reveals a common pattern of metabolic re-programming during invasion of three host plant species by Magnaporthe grisea
    David Parker
    Institute of Biological Environmental and Rural Sciences, Aberystwyth University, Penglais Campus, Aberystwyth, UK
    Plant J 59:723-37. 2009
    ..Taken together, our results suggest that fungal pathogens deploy a common metabolic re-programming strategy in diverse host species to suppress plant defence and colonize plant tissue...
  69. ncbi Broad connections in the Arabidopsis seed metabolic network revealed by metabolite profiling of an amino acid catabolism mutant
    Liping Gu
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA
    Plant J 61:579-90. 2010
    ..This combined targeted and non-targeted metabolite profiling approach is broadly applicable to the characterization of metabolic mutants, human disease studies and crop germplasm...
  70. pmc Rhabdomyosarcoma cells show an energy producing anabolic metabolic phenotype compared with primary myocytes
    Teresa W M Fan
    Department of Chemistry, University of Louisville, KY, USA
    Mol Cancer 7:79. 2008
    ..These include metabolites that report on the activity of glycolysis, Krebs' cycle, pentose phosphate pathway and pyrimidine biosynthesis...
  71. ncbi Human exhaled air analytics: biomarkers of diseases
    Bogusław Buszewski
    Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarin St, 87 100 Torun, Poland
    Biomed Chromatogr 21:553-66. 2007
    ..The diagnostic potential of different disease-marking substances in human breath for a selection of diseases and the clinical applications of breath analysis are discussed...
  72. ncbi Detection of lung cancer with volatile markers in the breath
    Michael Phillips
    Menssana Research Inc, Fort Lee, NJ 07024, USA
    Chest 123:2115-23. 2003
    ....
  73. ncbi Regulation of floral scent production in petunia revealed by targeted metabolomics
    Julian C Verdonk
    Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands
    Phytochemistry 62:997-1008. 2003
    ..Our results suggest that the circadian production of volatile benzenoids in petunia W115 is, at least partly, regulated at the transcript level...
  74. ncbi Chemical derivatization and mass spectral libraries in metabolic profiling by GC/MS and LC/MS/MS
    John M Halket
    Bourne Laboratory, Centre for Chemical and Bioanalytical Sciences, Royal Holloway, University of London, Egham, Surrey TW20 0EX, UK
    J Exp Bot 56:219-43. 2005
    ..The important role of mass spectral library creation and usage in these techniques is discussed and illustrated by examples...
  75. ncbi Diterpene resin acid biosynthesis in loblolly pine (Pinus taeda): functional characterization of abietadiene/levopimaradiene synthase (PtTPS-LAS) cDNA and subcellular targeting of PtTPS-LAS and abietadienol/abietadienal oxidase (PtAO, CYP720B1)
    Dae Kyun Ro
    Michael Smith Laboratories, and Departments of Botany and Forest Science, University of British Columbia, 321 2185 East Mall, Vancouver, BC, Canada
    Phytochemistry 67:1572-8. 2006
    ....
  76. ncbi Progressive peak clustering in GC-MS Metabolomic experiments applied to Leishmania parasites
    David P De Souza
    Department of Biochemistry and Molecular Biology, University of Melbourne, Parkville 3010, Australia
    Bioinformatics 22:1391-6. 2006
    ....
  77. ncbi Supervised pattern recognition procedures for discrimination of whiskeys from gas chromatography/mass spectrometry congener analysis
    Domingo González-Arjona
    Department of Physical Chemistry, University of Seville, 41012 Seville, Spain
    J Agric Food Chem 54:1982-9. 2006
    ..The possible cause of these findings is the strong influence of class inhomogeneities on the SIMCA performance. It is remarkable that in any case, all of the methodologies lead to zero FPs and FNs for the Irish whiskeys...
  78. pmc Loblolly pine abietadienol/abietadienal oxidase PtAO (CYP720B1) is a multifunctional, multisubstrate cytochrome P450 monooxygenase
    Dae Kyun Ro
    Michael Smith Laboratories and Departments of Botany and Forest Science, University of British Columbia, Vancouver, BC, Canada V6T 1Z4
    Proc Natl Acad Sci U S A 102:8060-5. 2005
    ....
  79. ncbi The use of vapor phase extraction in metabolic profiling of phytohormones and other metabolites
    Eric A Schmelz
    Center of Medical, Agricultural, and Veterinary Entomology USDA, Agricultural Research Service, 1600 1700 Southwest 23rd Drive, Gainesville, FL 32608, USA
    Plant J 39:790-808. 2004
    ..Directions for further method expansion are provided and include issues of recovery, derivatization, range of accessible analytes, optimization, reproducibility and future directions...
  80. ncbi Detecting simulated patterns of lung cancer biomarkers by random network of single-walled carbon nanotubes coated with nonpolymeric organic materials
    Gang Peng
    The Department of Chemical Engineering and Russell Barrie Nanotechnology Institute, Technion Israel Institute of Technology, Haifa 32000, Israel
    Nano Lett 8:3631-5. 2008
    ..The pattern compositions of the healthy and cancerous states were determined by gas-chromatography linked with mass-spectroscopy (GC-MS) analysis of real exhaled breath...
  81. ncbi Analysis of volatile organic compounds in the exhaled breath for the diagnosis of lung cancer
    Peter J Mazzone
    Respiratory Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    J Thorac Oncol 3:774-80. 2008
    ....
  82. pmc Noninvasive detection of lung cancer by analysis of exhaled breath
    Amel Bajtarevic
    Department of Operative Medicine, Innsbruck Medical University, A 6020 Innsbruck, Austria
    BMC Cancer 9:348. 2009
    ..An early non-invasive diagnosis of lung cancer would improve prognosis and enlarge treatment options. Analysis of exhaled breath would be an ideal diagnostic method, since it is non-invasive and totally painless...
  83. ncbi TD-GC-MS analysis of volatile metabolites of human lung cancer and normal cells in vitro
    Wojciech Filipiak
    Department of Operative Medicine, Center of Operative Medicine, Innsbruck Medical University, Innsbruck, Austria
    Cancer Epidemiol Biomarkers Prev 19:182-95. 2010
    ..These data suggest that certain groups of VOCs may be preferentially associated with the transformed phenotype...
  84. pmc Sniffing the unique "odor print" of non-small-cell lung cancer with gold nanoparticles
    Orna Barash
    Department of Chemical Engineering and Russell Berrie Nanotechnology Institute, Technion Israel Institute of Technology, Haifa 32000, Israel
    Small 5:2618-24. 2009
    ..In addition, such a tool might be used for the immediate diagnosis of fresh (frozen) tissues of lung cancer in operating rooms, where a dichotomic diagnosis is crucial to guide surgeons...
  85. ncbi The composition of alcohol products from markets in Lithuania and Hungary, and potential health consequences: a pilot study
    Dirk W Lachenmeier
    Chemisches und Veterinaruntersuchungsamt CVUA Karlsruhe, Germany
    Alcohol Alcohol 44:93-102. 2009
    ..The rates of alcohol-attributable mortality in Lithuania and Hungary have been shown to be higher than those in most other European countries. Quality of alcohol products is investigated as a possible explanation...
  86. ncbi Release of volatile organic compounds from the lung cancer cell line NCI-H2087 in vitro
    Andreas Sponring
    Department of Operative Medicine, Innsbruck Medical University, A 6020 Innsbruck, Austria
    Anticancer Res 29:419-26. 2009
    ..The aim of this work was to confirm the existence of volatile organic compounds (VOCs) specifically released by lung cancer cells...
  87. pmc Unbiased characterization of genotype-dependent metabolic regulations by metabolomic approach in Arabidopsis thaliana
    Miyako Kusano
    RIKEN Plant Science Center, 1 7 22, Yokohama, Kanagawa 230 0045, Japan
    BMC Syst Biol 1:53. 2007
    ..We investigated the metabolic network between wild-type and 2 mutant (methionine-over accumulation 1 [mto1] and transparent testa4 [tt4]) plants regarding the alteration of metabolite accumulation in Arabidopsis thaliana...
  88. pmc Monoterpenes in the glandular trichomes of tomato are synthesized from a neryl diphosphate precursor rather than geranyl diphosphate
    Anthony L Schilmiller
    Department of Biochemistry and Molecular Biology, Research Technology Support Facility, Michigan State University, East Lansing, MI 48824 1319, USA
    Proc Natl Acad Sci U S A 106:10865-70. 2009
    ..The data indicate that, contrary to the textbook view of geranyl diphosphate as the "universal" substrate of monoterpene synthases, in tomato glands neryl diphosphate serves as a precursor for the synthesis of monoterpenes...
  89. ncbi Dehydrosteroid measurements in maternal urine or serum for the prenatal diagnosis of Smith-Lemli-Opitz syndrome (SLOS)
    Cedric H L Shackleton
    Children s Hospital Oakland Research Institute, Oakland, California 94609, USA
    Am J Med Genet A 143:2129-36. 2007
    ..Urine testing performance prior to 14 weeks gestation appears promising, but reference data are sparse...
  90. pmc Discovery of non-aroclor PCB (3,3'-dichlorobiphenyl) in Chicago air
    Dingfei Hu
    Department of Civil and Environmental Engineering, IIHR Hydroscience and Engineering, University of Iowa, Iowa City, Iowa 52242, USA
    Environ Sci Technol 42:7873-7. 2008
    ..The wide distribution of PCB11 in Chicago air is consistent with volatilization of this compound from painted surfaces although the actual source of PCB11 is unknown...
  91. ncbi Metabolite profiling of mycorrhizal roots of Medicago truncatula
    Willibald Schliemann
    Department of Secondary Metabolism, Leibniz Institute of Plant Biochemistry, Weinberg 3, D 06120 Halle Saale, Germany
    Phytochemistry 69:112-46. 2008
    ..In conclusion, the present work shows for the first time that there are clear differences in development- and symbiosis-dependent primary and secondary metabolism of M. truncatula roots...
  92. ncbi Differential accumulation of volatile terpene and terpene synthase mRNAs during lavender (Lavandula angustifolia and L. x intermedia) inflorescence development
    Yann Guitton
    Universite de Lyon, F 69003, Lyon, France
    Physiol Plant 138:150-63. 2010
    ..Both molecular data and terpene analysis support the findings that changes in biosynthesis of terpene occurred during inflorescence development...
  93. pmc A dynamic programming approach for the alignment of signal peaks in multiple gas chromatography-mass spectrometry experiments
    Mark D Robinson
    The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3050, Australia
    BMC Bioinformatics 8:419. 2007
    ..Automated retention time correction is particularly important in non-targeted profiling studies...
  94. ncbi Antipyretic, analgesic, and anti-inflammatory activities of the seaweed Sargassum fulvellum and Sargassum thunbergii in mice
    J Y Kang
    Department of Biotechnology, Pukyong National University, Namku, Busan 608 737, Republic of Korea
    J Ethnopharmacol 116:187-90. 2008
    ..1%. No acute toxicity was observed after p.o. administration of each extract (5 g/kg bw). These findings are consistent with various claims that these seaweeds can be used as remedies for inflammation-related symptoms...
  95. ncbi Exhaled breath analysis: novel approach for early detection of lung cancer
    Hiang Ping Chan
    Faculty of Medicine, University of New South Wales, Department of Respiratory Medicine, Prince of Wales Hospital, Randwick, NSW 2031, Australia
    Lung Cancer 63:164-8. 2009
    ..However, since EBC is a simple, non-invasive technique that can be easily performed, even in ill patients, it has the potential to be validated for use in screening for the early diagnosis of lung cancer...
  96. ncbi Photodegradation of organophosphorus insecticides - investigations of products and their toxicity using gas chromatography-mass spectrometry and AChE-thermal lens spectrometric bioassay
    M Bavcon Kralj
    Laboratory for Environmental Research, University of Nova Gorica, Vipavska 13, P O Box 301, 5001 SI, Nova Gorica, Slovenia
    Chemosphere 67:99-107. 2007
    ..The presented results demonstrate the importance of toxicity monitoring during the degradation of OPs in processes of waste water remediation, before releasing it into the environment...
  97. ncbi Classification of weathered petroleum oils by multi-way analysis of gas chromatography-mass spectrometry data using PARAFAC2 parallel factor analysis
    Diako Ebrahimi
    School of Chemistry, University of New South Wales, Sydney, NSW 2052, Australia
    J Chromatogr A 1166:163-70. 2007
    ..This lead to the identification of diagnostic compounds in the studied diesel oil samples...
  98. ncbi Kinetics of labelling of organic and amino acids in potato tubers by gas chromatography-mass spectrometry following incubation in (13)C labelled isotopes
    Ute Roessner-Tunali
    Department of Molecular Physiology, Max Planck Institut fur Molekulare Pflanzenphysiologie, Am Muhlenberg 1, 14476 Golm, Germany
    Plant J 39:668-79. 2004
    ..The fact that this method is able to rapidly yield further comprehensive information into primary metabolism illustrates its power as a further phenotyping tool for the analysis of plant metabolism...
  99. ncbi GC-MS libraries for the rapid identification of metabolites in complex biological samples
    Nicolas Schauer
    Max Planck Institute of Plant Molecular Physiology, Am Muehlenberg 1, D 14476 Golm, Germany
    FEBS Lett 579:1332-7. 2005
    ..csbdb.mpimp-golm.mpg.de/gmd.html). This resource should ameliorate many of the problems that each laboratory will face both for the initial establishment of metabolome analysis and for its maintenance at a constant sample throughput...
  100. pmc Biosynthesis and emission of terpenoid volatiles from Arabidopsis flowers
    Feng Chen
    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109, USA
    Plant Cell 15:481-94. 2003
    ....
  101. ncbi Quantitative analysis of morphine in human plasma by gas chromatography-negative ion chemical ionization mass spectrometry
    H J Leis
    University Children s Hospital, Department of Analytical Biochemistry and Mass Spectrometry, Graz, Austria
    J Chromatogr B Biomed Sci Appl 744:113-9. 2000
    ..5% (14.54 ng/ml). Accuracy showed deviations of -9.3% (2.85 ng/ml) and -4.2% (14.25 ng/ml). The method is rugged and robust and has been applied to the batch analysis of morphine during pharmacokinetic profiling of the drug...

Research Grants65

  1. Maternal Obesity and Gestational Diabetes: Impact on Metabolome
    William L Lowe; Fiscal Year: 2013
    ..Together, these studies will have an important impact on our understanding of the metabolic underpinnings of the increased risk of metabolic diseases in newborns of mothers with hyperglycemia and/or obesity. ..
  2. Roles of pulmonary and hepatic P4501A enzymes in hyperoxic lung injury in mice
    Bhagavatula Moorthy; Fiscal Year: 2010
    ..The long-term of this proposal is to develop rational strategies for the prevention and/or treatment of chronic lung diseases in infants and adults suffering from respiratory distress and lung dysfunction. ..
  3. Continuation of FERN Activities at the Connecticut Agricultural Experiment Statio
    LAURIE J PETERSON-WRIGHT; Fiscal Year: 2013
    ..The CAES Department of Analytical Chemistry has demonstrated the expertise, capability, and adaptability to fully meet and exceed these program needs. ..
  4. REGULATION OF 15N UREA ISOTOPOMERS PRODUCTION
    Itzhak Nissim; Fiscal Year: 2013
    ..Our preliminary results suggest that such treatment is feasible. The outcome of the propose study may provide new guidelines for evaluation, diagnosis and treatment of defective urea synthesis in NAFLD or MS. ..
  5. Cardiac Autonomic Neuropathy and Myocardial Dysfunction in Type 1 Diabetes
    RODICA BUSUI POP-BUSUI; Fiscal Year: 2013
    ..These studies will help elucidate mechanisms of myocardial dysfunction in T1DM with ultimate goal of designing future studies implementing therapeutic strategies aimed at preventing increased cardiovascular risk in patients with T1DM. ..
  6. REGULATION OF 15N UREA ISOTOPOMERS PRODUCTION
    Itzhak Nissim; Fiscal Year: 2009
    ..and disease states and thus may lead to development of a novel intervention to improve urea synthesis and detoxification of ammonia associated with fatty liver or in cases such as drug-induced inhibition of urea synthesis ..
  7. Phthalate Exposure and Pregnancy Outcome
    T Peter Stein; Fiscal Year: 2010
    ..The findings will have ramifications for public health, for the packaging trade and for the chemical industry. ..
  8. GLUCOSE/SECRETAGOGUE METABOLISM IN PANCREATIC ISLETS
    MICHAEL JOHN MACDONALD; Fiscal Year: 2013
    ..Aim 2B is to continue to study intra- and extra- mitochondrial metabolic enzymes that are decreased in islets of rodents and humans with type 2 diabetes and factors that might regulate their expression. ..
  9. Regulation of Muscle ATP Synthase Beta Subunit Metabolism in Obesity
    Christos S Katsanos; Fiscal Year: 2013
    ..Further, they will provide scientific knowledge to base lifestyle interventions to improve muscle ATP turnover in obese individuals. ..
  10. GABA-active neurosteroids in contextual fear responses
    Graziano Pinna; Fiscal Year: 2011
    ....
  11. Hydrogen Sulfide in Newborn Cerebral Circulation
    Charles W Leffler; Fiscal Year: 2013
    ..The gasotransmitter, H2S, will certainly prove to be one of these major mechanisms of which greater understanding is urgently needed. ..
  12. SOLID STATE RADIATION CHEMISTRY OF DNA
    Jeffrey J Hayes; Fiscal Year: 2012
    ....
  13. Intralumental Effect on Cholesterol Absorption/Synthesis
    James Heubi; Fiscal Year: 2009
    ..Integration of animal/human techniques will provide tools to characterize the role of modifications of the intraluminal environment on cholesterol solubilization and human cholesterol absorption and synthesis. ..
  14. Mycobacterium Tuberculosis Lipoarabinomannan In Vivo
    Libin Shi; Fiscal Year: 2013
    ..The definition of the terminal ends of LAM in vivo will impact the future when a sensitive and specific anti-LAM antibody can be generated based on this study to enhance LAM based existing assays for use in resource poor regions. ..
  15. Fetal Exposure to Environmental Toxicants and Child Outcome
    ENRIQUE MAPUA OSTREA; Fiscal Year: 2012
    ....
  16. CONTROL OF NEONATAL CIRCULATION
    Charles W Leffler; Fiscal Year: 2013
    ..These studies are important because disorders of the cerebral circulation in the newborn period are major causes of sickness and death and can result in lifelong disabilities in survivors. ..
  17. Endocannabinoid signaling and aging
    MATTHEW SIMON GILL; Fiscal Year: 2013
    ..Together these studies will provide an opportunity to define the ways in which the endocannabinoid system can be manipulated to promote healthy aging as well as identifying novel therapeutic strategies for human aging and disease. ..
  18. Defining the Impact of Lipid Synthesis and Turnover on Aging in C. elegans
    CARISSA OLSEN; Fiscal Year: 2013
    ..The goal of this proposal is to define the genetic regulators and pathways that influence membrane aging and, in doing so, contribute to the understanding of how membrane biology impacts the aging process. ..
  19. Myxococcus xanthus signaling and cell fate
    LAWRENCE JOSEPH SHIMKETS; Fiscal Year: 2013
    ..Such lipids are an essential feature of mammalian development and homeostasis. This system may reveal novel regulatory strategies that are as yet undiscovered in mammals. ..
  20. NEUROSTEROID MODULATION OF ETHANOL WITHDRAWAL SEVERITY
    David J Rossi; Fiscal Year: 2013
    ..Thus, the proposed experiments will establish the importance of altered hippocampal neurosteroid composition and GABAA receptor pharmacology in mediating ethanol withdrawal severity. ..
  21. Food Safety and Security Monitoring Project (U18), FERN
    Abul Anisuzzaman; Fiscal Year: 2013
    ..The project narrative provides detailed information to address the complementary needs of the FDA and shows our thorough commitment to the FERN Chemistry Program. ..
  22. Saliva Analyzer for Diagnosing SRD Patient Drug Use
    Stuart Farquharson; Fiscal Year: 2012
    ....
  23. PLASMA PROTEINS: MARKERS OF CHEMICAL EXPOSURE
    GHULAM ANSARI; Fiscal Year: 1991
    ..The proposed screening and chemoprevention studies to be conducted in China will be a collaborative project by investigators in China and the United States...
  24. New Hampshire Food Safety and Security Monitoring Project - Chemistry
    CHRISTINE LOUISE BEAN; Fiscal Year: 2013
    ..abstract_text> ..
  25. Neural mechanisms of olfactory reward value processing in the human brain
    James D Howard; Fiscal Year: 2013
    ..Given its unique anatomical organization and intimate affiliation with limbic emotional brain regions, the olfactory system presents an excellent model system for examining appetitive learning processes. ..
  26. Clinical Validation of a Biofilm Signature for Early Caries Activity Assessment
    ANDREA GONCALVES FERREIRA ZANDONA; Fiscal Year: 2012
    ..Soini and Novotny from the IU Department of Chemistry, and Institute for Pheromone Research as well as current collaborators in the University of Puerto Rico, where our study population is located. ..
  27. FDA FERN Chemistry Cooperative Agreement 2010
    STEVEN SOBEK; Fiscal Year: 2013
    ..2. The use of Liquid Chromatography\Mass Spectrometry (LC\MS) analysis. 3. The use of Inductively Coupled Plasma\Mass Spectrometry (ICP\MS) analysis. 4. The use of Enzyme Linked Immunosorbent Assay (ELISA) analysis. ..
  28. PROSTAGLANDINS AND LOCAL CONTROL OF THE CIRCULATION
    ALAN NIES; Fiscal Year: 1990
    ....
  29. MASS SPECTROMETER SYSTEM AND ACCESSORIES
    ALAN NIES; Fiscal Year: 1990
    ..For this proposal, the University has committed funds for renovation of space and maintenance of the requested instrument in order to ensure its optimal utilization...
  30. Effects of Natural Agents with Fluoride on Caries
    Hyun Koo; Fiscal Year: 2004
    ....
  31. MYCOBACTERIAL CELL WALL AND DRUG DISCOVERY
    Patrick Brennan; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  32. Biosynthesis of Taxol Precursors in Transgenic Moss
    Aldwin Anterola; Fiscal Year: 2009
    ..This process, however, is very expensive and do not provide a steady supply of the drug. The goal of this project is to develop an economical method of producing Taxol and Taxotere using moss bioreactors. ..
  33. CONTROL OF ENDOCANNABINOID RELEASE IN VIVO
    Daniele Piomelli; Fiscal Year: 2003
    ..Moreover, by uncovering the neurochemical interactions between cocaine and the endogenous cannabinoids, our studies will contribute to the general understanding of substance abuse and neuropsychiatric disorders. ..
  34. TERPENE CYCLASES: FUNCTIONAL DOMAINS AND STRUCTURES
    JOSEPH NOEL; Fiscal Year: 2003
    ..Our current objective is to understand the regiochemical and stereochemical principles that govern the biosynthesis of pharmaceutically useful terpenoids. ..
  35. MEMBRANE LIPIDS AND THEIR POTENTIAL ROLE IN LUPUS
    THOMAS SANTORO; Fiscal Year: 1992
    ....
  36. MEIBOMIAN KERATOCONJUNCTIVITIS
    James McCulley; Fiscal Year: 1999
    ..Clinical trials will eventually be performed, based on these evaluations, thus permitting the ophthalmologist to select specific therapeutic treatments based on clinical signs and simple biochemical or microbial assays. ..
  37. TWO TYPES OF MONOAMINE OXIDASE
    Jean Shih; Fiscal Year: 2007
    ..unreadable] [unreadable] These studies are important for both basic neuropharmacology and clinical[unreadable] research. ..
  38. DEVELOPMENTAL NEUROCHEMISTRY OF BUPRENORPHINE
    Susan Robinson; Fiscal Year: 1999
    ....
  39. Molecular examination of the identity and activity of Po
    EUGENE MADSEN; Fiscal Year: 2004
    ..abstract_text> ..
  40. DIAGNOSTIC VIABILITY OF UMBILICAL CORD SPECIMENS
    Charles Plate; Fiscal Year: 2007
    ..unreadable]..
  41. Kinetics of Human Homocysteine Metabolism
    Glen Duncan; Fiscal Year: 2004
    ..g., exercise training and pharmacotherapy) on diabetes prevention and therapy. ..
  42. FUEL METABOLISM DURING AND AFTER EXERCISE IN DIABETES
    John Devlin; Fiscal Year: 1991
    ....
  43. DEFECTIVE BILE ACID SYNTHESIS IN CHILDHOOD CONSTIPATION
    ALAN HOFMANN; Fiscal Year: 2005
    ..Results of this study should lead to clinical trials that test the efficacy of oral primary bile acids for constipation caused by defective bile acid biosynthesis. ..
  44. DEGENERATION--EPINEPHRINE NEURONS IN ALZHEIMER'S DISEASE
    William Burke; Fiscal Year: 1993
    ..The Epi neurotransmitter system is affected late in AD. While Epi neurons are undergoing degeneration there is no cell loss making it an ideal system to look at the biochemical mechanisms of neuronal degeneration...
  45. GENERAL CLINICAL RESEARCH CENTER
    John Daly; Fiscal Year: 2002
    ..The GCRC at Temple University has been a major source of strength for the School of Medicine in the past and hopefully will continue in this role in the future. ..
  46. INTERACTION OF DRUGS WITH BRAIN ACETYLCHOLINE
    DONALD JENDEN; Fiscal Year: 1992
    ....
  47. DIETARY INTERVENTION--WOMEN HIGH RISK FOR BREAST CANCER
    Zora Djuric; Fiscal Year: 2002
    ..The use of biomarkers will allow to evaluate the cancer-protective effects of dietary modulation over a relatively short period of time. ..
  48. EARLY URINE B12 DEFICIENCY DETECTION IN ELDERLY
    ERIC NORMAN; Fiscal Year: 1990
    ....
  49. PATHOGENESIS OF COCAETHYLENE-INDUCED VASCULITIS
    DANYEL TACKER; Fiscal Year: 2004
    ..The proposed Aims will provide further insight into the pathogenic sequelae resulting from production of CE in humans. ..
  50. Non-Clinical ADME Studies
    Emil T Lin; Fiscal Year: 2009
    ..abstract_text> ..
  51. MECHANISMS OF CELLULAR CONTROL OF NITROGEN LIMITATION
    Mary Allen; Fiscal Year: 1990
    ....
  52. In Vivo Metabolism of Pulmonary Surfactant in Infants
    Aaron Hamvas; Fiscal Year: 2005
    ..abstract_text> ..
  53. Liver triglyceride metabolism in NASH
    Elizabeth Parks; Fiscal Year: 2002
    ..In combination with the clinical data obtained, an understanding of the contributions of FA sources to liver TG will aid in the development of future treatment strategies for this disease. ..
  54. FUNDAMENTAL STUDIES FOR BIOANALYTICAL CHEMISTRY
    RICHARD BROWNER; Fiscal Year: 1990
    ..The ultimate goal would be to develop an effective combined MAGIC-LC/FT-IR/MS system. Such a system would provide enormously powerful identifying power for mixtures of biochemically important molecules...
  55. FETAL COCAINE EXPOSURE; EFFECT ON NEONATAL BREATHING
    GEORGE OLSEN; Fiscal Year: 1991
    ..These studies are especially important since infants of substance-abusing mothers (ISAM) including cocaine-only users, have abnormal sleeping ventilatory patterns and may have an increased risk of sudden infant death syndrome (SIDS)...
  56. BIOLOGICAL DISPOSITION OF NARCOTIC ANALGESICS IN MAN
    CARL HUG; Fiscal Year: 1980
    ..to investigate the basis of interactions of narcotic analgesics with other drugs, and 4. to recommend modifications of dosage regimens in various disease states (e.g., drug and alcohol abuse, liver failure). ..
  57. Methylphenidate-Ethanol Interaction in ADHD and Coabuse
    Kennerly Patrick; Fiscal Year: 2003
    ..g., dextroamphetamine vs. MPH; dI-MPH vs. d-MPH; immediate-release MPH vs. extended-release MPH; or adjustment of MPH. ..
  58. GENERAL CLINICAL RESEARCH CENTER
    NEIL CHERNIACK; Fiscal Year: 1990
    ....
  59. PROTEIN PRIMARY STRUCTURE THROUGH DIPEPTIDE ANALYSIS
    Richard Caprioli; Fiscal Year: 1980
    ..Several specific research projects which require this type of information will be investigated. ..
  60. SMOKING AND ATHEROSCLEROSIS: MECHANISMS AND MODULATION
    Anne Burke; Fiscal Year: 2005
    ..We expect that smoking reduction and cessation and the administration of antioxidants and aspirin will improve these parameters towards the levels of healthy volunteers and that the effects will be additive. ..
  61. MODEL RESOURCE FOR HUMAN INHERITED DISEASES
    Philip Wood; Fiscal Year: 1990
    ..This technique could revolutionize animal model development with wide application to any disorder that has the associated cloned DNA sequence available...
  62. ENDOGENOUS REGULATION OF LEUKOTRIENE SYNTHESIS
    STEVEN FEINMARK; Fiscal Year: 1990
    ..Regulation and control of PMNL migration and vascular permeability are critical to homeostasis and in all inflammatory reactions...
  63. TAU GLYCOSYLATION IN ALZHEIMERS DISEASE
    Cheng Xin Gong; Fiscal Year: 2001
    ..These studies will elucidate the abnormal tau glycosylation and its impact on the formation of PHF tangles and, therefore, should provide a new inside view of the mechanism of Alzheimer neurofibrillary degeneration. ..
  64. MONOAMINE PREDICTORS OF DRUG ABUSE/SOCIOPATHY
    Roy King; Fiscal Year: 1992
    ..In addition, psychopharmacological interventions that raise levels of PAA, such as the MAO-B inhibitors, may potentially help compensate for this dysfunctional impulsivity...
  65. CHEMICAL DIGESTION-GC/MS ANALYSIS OF DNA ADDUCTS
    Roger Giese; Fiscal Year: 1990
    ..The proposed work is intended to speed up advances in quantitative risk assessment. Kurt Randerath at Baylor, and Douglas Rickert and James Swenberg at CIIT, are the collaborators on this project...