microbubbles

Summary

Summary: Very small encapsulated gas bubbles (diameters of micrometers) that can be used in diagnostic and therapeutic applications. Upon exposure to sufficiently intense ultrasound, microbubbles will cavitate, rupture, disappear, release gas content, etc. Such characteristics of the microbubbles can be used to enhance diagnostic tests, dissolve blood clots, and deliver drugs or genes for therapy.

Top Publications

  1. ncbi Ultrasound targeted microbubble destruction increases capillary permeability in hepatomas
    Raffi Bekeredjian
    Internal Medicine III, University of Heidelberg, Heidelberg, Germany
    Ultrasound Med Biol 33:1592-8. 2007
  2. ncbi Ultrasound-responsive polymer-coated microbubbles that bind and protect DNA
    Ine Lentacker
    Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmacy, Ghent University, Harelbekestraat 72, B 9000 Ghent, Belgium
    Langmuir 22:7273-8. 2006
  3. ncbi Ultrasound microbubble contrast agents: fundamentals and application to gene and drug delivery
    Katherine Ferrara
    Department of Biomedical Engineering, University of California, Davis, California 95616 8686, USA
    Annu Rev Biomed Eng 9:415-47. 2007
  4. pmc Ultrasound molecular imaging of tumor angiogenesis with an integrin targeted microbubble contrast agent
    Christopher R Anderson
    Targeson, Inc, San Diego, CA, USA
    Invest Radiol 46:215-24. 2011
  5. pmc Advances in ultrasound mediated gene therapy using microbubble contrast agents
    Shashank R Sirsi
    Department of Mechanical Engineering, University of Colorado, Boulder, CO 80309, USA
    Theranostics 2:1208-22. 2012
  6. ncbi Ultrasound microbubbles for molecular diagnosis, therapy, and theranostics
    Fabian Kiessling
    Department of Experimental Molecular Imaging, RWTH Aachen University, Aachen, Germany
    J Nucl Med 53:345-8. 2012
  7. ncbi Focused ultrasound-induced stimulation of microbubbles augments site-targeted engraftment of mesenchymal stem cells after acute myocardial infarction
    Alexander Ghanem
    Department of Medicine Cardiology, University of Bonn, Sigmund Freud Str 25, 53105 Bonn, Germany
    J Mol Cell Cardiol 47:411-8. 2009
  8. ncbi Improving the cardio protective effect of aFGF in ischemic myocardium with ultrasound-mediated cavitation of heparin modified microbubbles: preliminary experiment
    Ying Zheng Zhao
    Wenzhou Medical College, Wenzhou City, Zhejiang Province, China
    J Drug Target 20:623-31. 2012
  9. ncbi Preparation of nanobubbles for ultrasound imaging and intracelluar drug delivery
    Ye Wang
    Zhejiang California International Nanosystems Institute Molecular Imaging Platform, Zhejiang University, 268 Kaixuan Road, Hangzhou 310029, PR China
    Int J Pharm 384:148-53. 2010
  10. pmc Microbubbles in ultrasound-triggered drug and gene delivery
    Sophie Hernot
    Cardiovascular Division, Department of Medicine, University of Virginia School of Medicine, P O Box 800158, RM1026, Hospital Drive, Cobb Hall, Charlottesville, VA 22908 0158, USA
    Adv Drug Deliv Rev 60:1153-66. 2008

Detail Information

Publications317 found, 100 shown here

  1. ncbi Ultrasound targeted microbubble destruction increases capillary permeability in hepatomas
    Raffi Bekeredjian
    Internal Medicine III, University of Heidelberg, Heidelberg, Germany
    Ultrasound Med Biol 33:1592-8. 2007
    ..Another 12 rats received DNA-loaded microbubbles by UTMD to one tumor, encoding for luciferase...
  2. ncbi Ultrasound-responsive polymer-coated microbubbles that bind and protect DNA
    Ine Lentacker
    Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmacy, Ghent University, Harelbekestraat 72, B 9000 Ghent, Belgium
    Langmuir 22:7273-8. 2006
    Ultrasound in combination with microbubbles has recently been considered by gene delivery scientists to be an interesting approach to enhance gene transfer into cells...
  3. ncbi Ultrasound microbubble contrast agents: fundamentals and application to gene and drug delivery
    Katherine Ferrara
    Department of Biomedical Engineering, University of California, Davis, California 95616 8686, USA
    Annu Rev Biomed Eng 9:415-47. 2007
    This review offers a critical analysis of the state of the art of medical microbubbles and their application in therapeutic delivery and monitoring...
  4. pmc Ultrasound molecular imaging of tumor angiogenesis with an integrin targeted microbubble contrast agent
    Christopher R Anderson
    Targeson, Inc, San Diego, CA, USA
    Invest Radiol 46:215-24. 2011
    ..Our objectives are to develop a robust ultrasound contrast agent platform using microbubbles (MB) to which targeting ligands can be conjugated by biocompatible, covalent conjugation chemistry, and to ..
  5. pmc Advances in ultrasound mediated gene therapy using microbubble contrast agents
    Shashank R Sirsi
    Department of Mechanical Engineering, University of Colorado, Boulder, CO 80309, USA
    Theranostics 2:1208-22. 2012
    ..The physical response of microbubbles in an ultrasound field can mechanically perturb blood vessel walls and cell membranes, enhancing drug ..
  6. ncbi Ultrasound microbubbles for molecular diagnosis, therapy, and theranostics
    Fabian Kiessling
    Department of Experimental Molecular Imaging, RWTH Aachen University, Aachen, Germany
    J Nucl Med 53:345-8. 2012
    ..b>Microbubbles as vascular contrast agents improve the detection and characterization of cancerous lesions, inflammatory ..
  7. ncbi Focused ultrasound-induced stimulation of microbubbles augments site-targeted engraftment of mesenchymal stem cells after acute myocardial infarction
    Alexander Ghanem
    Department of Medicine Cardiology, University of Bonn, Sigmund Freud Str 25, 53105 Bonn, Germany
    J Mol Cell Cardiol 47:411-8. 2009
    ..Highly focused ultrasound-mediated stimulation of microbubbles (hf-UMS) allows locoregional pre-treatment of target tissue...
  8. ncbi Improving the cardio protective effect of aFGF in ischemic myocardium with ultrasound-mediated cavitation of heparin modified microbubbles: preliminary experiment
    Ying Zheng Zhao
    Wenzhou Medical College, Wenzhou City, Zhejiang Province, China
    J Drug Target 20:623-31. 2012
    Ultrasound (US)-mediated cavitation of microbubbles has evolved into a new tool for organ-specific gene and drug delivery...
  9. ncbi Preparation of nanobubbles for ultrasound imaging and intracelluar drug delivery
    Ye Wang
    Zhejiang California International Nanosystems Institute Molecular Imaging Platform, Zhejiang University, 268 Kaixuan Road, Hangzhou 310029, PR China
    Int J Pharm 384:148-53. 2010
    ..It can be concluded that the nanobubble formulation is a promising approach for both ultrasound imaging and drug delivery enhancing...
  10. pmc Microbubbles in ultrasound-triggered drug and gene delivery
    Sophie Hernot
    Cardiovascular Division, Department of Medicine, University of Virginia School of Medicine, P O Box 800158, RM1026, Hospital Drive, Cobb Hall, Charlottesville, VA 22908 0158, USA
    Adv Drug Deliv Rev 60:1153-66. 2008
    Ultrasound contrast agents, in the form of gas-filled microbubbles, are becoming popular in perfusion monitoring; they are employed as molecular imaging agents...
  11. pmc Blood-brain barrier disruption induced by focused ultrasound and circulating preformed microbubbles appears to be characterized by the mechanical index
    Nathan Mcdannold
    Department of Radiology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
    Ultrasound Med Biol 34:834-40. 2008
    ..It also suggests that lower frequency sonication produces less red blood cell extravasation per unit area...
  12. ncbi Microbubble size isolation by differential centrifugation
    Jameel A Feshitan
    Chemical Engineering, Columbia University, 500 W 120 ST, New York, NY 10027, USA
    J Colloid Interface Sci 329:316-24. 2009
    b>Microbubbles used as contrast agents for ultrasound imaging, vectors for targeted drug delivery and vehicles for metabolic gas transport require better size control for improved performance...
  13. ncbi SPIO-conjugated, doxorubicin-loaded microbubbles for concurrent MRI and focused-ultrasound enhanced brain-tumor drug delivery
    Ching Hsiang Fan
    Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, No 101 Section 2 Kuang Fu Road, Hsinchu, Taiwan, ROC
    Biomaterials 34:3706-15. 2013
    ..barrier (BBB) can be temporarily and locally opened by focused ultrasound (FUS) in the presence of circulating microbubbles (MBs)...
  14. ncbi Cavitation microstreaming and stress fields created by microbubbles
    James Collis
    Department of Mechanical Engineering, University of Melbourne, VIC 3010, Melbourne, Australia
    Ultrasonics 50:273-9. 2010
    Cavitation microstreaming plays a role in the therapeutic action of microbubbles driven by ultrasound, such as the sonoporative and sonothrombolytic phenomena. Microscopic particle-image velocimetry experiments are presented...
  15. ncbi Ultrasound-targeted gene delivery induces angiogenesis after a myocardial infarction in mice
    Hiroko Fujii
    Division of Cardiovascular Surgery, Toronto General Research Institute, Toronto General Hospital, Toronto, Ontario, Canada
    JACC Cardiovasc Imaging 2:869-79. 2009
    ..This study evaluated the capacity of ultrasound-targeted microbubble destruction (UTMD) to deliver angiogenic genes, improve perfusion, and recruit progenitor cells after a myocardial infarction (MI) in mice...
  16. ncbi Cellular mechanisms of the blood-brain barrier opening induced by ultrasound in presence of microbubbles
    Nickolai Sheikov
    Department of Radiology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Ultrasound Med Biol 30:979-89. 2004
    ..free passage through the injured endothelium (with the higher power sonications). These findings could be considered in further development of the strategy for drug delivery to brain parenchyma...
  17. ncbi Reversible blood-brain barrier disruption by repeated transcranial focused ultrasound allows enhanced extravasation
    Feng Yi Yang
    Department of Biomedical Imaging and Radiological Sciences, School of Biomedical Science and Engineering, National Yang Ming University, Taipei, Taiwan
    J Control Release 150:111-6. 2011
    ..This noninvasive technology offers the possibility of controlling the extent of drug delivery by means of repeated treatment and adjusting the duration and interval between sonications...
  18. pmc Targeted gene transfection from microbubbles into vascular smooth muscle cells using focused, ultrasound-mediated delivery
    Linsey C Phillips
    Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA
    Ultrasound Med Biol 36:1470-80. 2010
    ..smooth muscle cells using ultrasound triggered delivery of plasmid DNA from electrostatically coupled cationic microbubbles. Microbubbles carrying reporter plasmid DNA were acoustically ruptured in the vicinity of smooth muscle cells ..
  19. ncbi Microbubble-enhanced US in body imaging: what role?
    Stephanie R Wilson
    Department of Diagnostic Imaging, Foothills Medical Centre, University of Calgary, Calgary, AB, Canada
    Radiology 257:24-39. 2010
    ..Microbubble contrast agents are easy to use and robust; their use poses no risk of nephrotoxicity and requires no ionizing radiation. CE US plays a vital and expanding role that improves management and patient care...
  20. ncbi The role of caveolin-1 in blood-brain barrier disruption induced by focused ultrasound combined with microbubbles
    Jinmu Deng
    Department of Neurosurgery, Second Affiliated Hospital, Chongqing Medical University, Chongqing, China
    J Mol Neurosci 46:677-87. 2012
    ..whether disrupting the blood-brain barrier (BBB) in rats by applying focused ultrasound (FUS) combined with microbubbles induced changes in the density of caveolae and/or the expression of the structural protein caveolin-1...
  21. ncbi Enhanced homing of mesenchymal stem cells to the ischemic myocardium by ultrasound-targeted microbubble destruction
    Shigen Zhong
    Institute of Ultrasound Imaging, Chongqing Medical University, Chongqing 400010, China
    Ultrasonics 52:281-6. 2012
    ..Myocardial microenvironment changes caused by UTMD may promote the homing of MSCs to the ischemic myocardium. This non-invasive technique may be a promising method for cardiac cell transplantation therapy...
  22. ncbi Elucidating the mechanisms behind sonoporation with adeno-associated virus-loaded microbubbles
    Bart Geers
    Ghent Research Group on Nanomedicines, Lab of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000 Gent, Belgium
    Mol Pharm 8:2244-51. 2011
    b>Microbubbles are Food and Drug Administration (FDA) approved contrast agents for ultrasound imaging. It has been reported that applying ultrasound on drug-loaded microbubbles facilitates drug uptake by cells, due to so-named sonoporation...
  23. ncbi Magnetite nanoparticles can be coupled to microbubbles to support multimodal imaging
    Torkel B Brismar
    Department of Clinical Science, Intervention and Technology at Karolinska Institutet, Division of Medical Imaging and Technology, Stockholm, Sweden
    Biomacromolecules 13:1390-9. 2012
    b>Microbubbles (MBs) are commonly used as injectable ultrasound contrast agent (UCA) in modern ultrasonography. Polymer-shelled UCAs present additional potentialities with respect to marketed lipid-shelled UCAs...
  24. ncbi Bioeffects of ultrasound-stimulated microbubbles on endothelial cells: gene expression changes associated with radiation enhancement in vitro
    Azza A Al-Mahrouki
    Radiation Oncology and Imaging Research, Sunnybrook Health Sciences Centre, Toronto, Ontario, Canada
    Ultrasound Med Biol 38:1958-69. 2012
    ..Human-endothelial cells were exposed to ultrasound and microbubbles, radiation or combinations of ultrasound, microbubbles and radiation...
  25. pmc Temporary disruption of the blood-brain barrier by use of ultrasound and microbubbles: safety and efficacy evaluation in rhesus macaques
    Nathan Mcdannold
    Department of Radiology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cancer Res 72:3652-63. 2012
    ..These results therefore support clinical testing of this noninvasive-targeted drug delivery method...
  26. ncbi The use of cationic microbubbles to improve ultrasound-targeted gene delivery to the ischemic myocardium
    Lu Sun
    Department of Cardiovascular Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China
    Biomaterials 34:2107-16. 2013
    ..UTMD therapy with this CMB provides an efficient platform for the targeted delivery of factors required to regenerate the ischemic heart and preserve cardiac function...
  27. pmc Ultrasound contrast microbubbles in imaging and therapy: physical principles and engineering
    Shengping Qin
    Department of Biomedical Engineering, University of California, 451 East Health Sciences Drive, Davis, CA 95616, USA
    Phys Med Biol 54:R27-57. 2009
    ..Over this period, stabilizing shells and low diffusivity gas materials have been incorporated in microbubbles, extending stability in vitro and in vivo...
  28. pmc Analysis of in vitro transfection by sonoporation using cationic and neutral microbubbles
    Jose L Tlaxca
    Department of Biomedical Engineering, University of Virginia, Charlottesville, VA 22908, USA
    Ultrasound Med Biol 36:1907-18. 2010
    ..Moreover, direct conjugation of plasmid to the microbubble did not appear to significantly enhance transfection efficiency under the examined conditions, although this strategy may be important for targeted transfection in vivo...
  29. ncbi Development of polymeric microbubbles targeted to prostate-specific membrane antigen as prototype of novel ultrasound contrast agents
    Vanna Sanna
    Porto Conte Ricerche, Località Tramariglio, 07041 Alghero, Sassari, Italy
    Mol Pharm 8:748-57. 2011
    Ultrasound-targeted microbubbles (MBs) offer new opportunities to enhance the capabilities of diagnostic ultrasound (US) imaging to specific pathological tissue...
  30. ncbi Ultrasound and microbubble-targeted delivery of macromolecules is regulated by induction of endocytosis and pore formation
    Bernadet D M Meijering
    Department of Clinical Pharmacology, University Medical Center Groningen, University of Groningen, The Netherlands
    Circ Res 104:679-87. 2009
    Contrast microbubbles in combination with ultrasound (US) are promising vehicles for local drug and gene delivery. However, the exact mechanisms behind intracellular delivery of therapeutic compounds remain to be resolved...
  31. pmc Dual targeting improves microbubble contrast agent adhesion to VCAM-1 and P-selectin under flow
    E A Ferrante
    Department of Biomedical Engineering, University of Virginia, Charlottesville, VA 22908, USA
    J Control Release 140:100-7. 2009
    ..When microbubbles were perfused through flow chambers at 5 x 10(6) microbubbles/ml (wall shear stress from 1...
  32. pmc Tumor angiogenic marker expression levels during tumor growth: longitudinal assessment with molecularly targeted microbubbles and US imaging
    Nirupama Deshpande
    Molecular Imaging Program at Stanford, Department of Radiology, Stanford University School of Medicine, 300 Pasteur Dr, Room H1307, Stanford, CA 94305 5621, USA
    Radiology 258:804-11. 2011
    To evaluate the use of molecularly targeted microbubbles (MBs) and ultrasonography (US) in the noninvasive assessment of the level of expression of three angiogenic markers, α(v)β(3) integrin, endoglin, and vascular endothelial growth ..
  33. pmc The mechanism of interaction between focused ultrasound and microbubbles in blood-brain barrier opening in mice
    Yao Sheng Tung
    Department of Biomedical Engineering, Columbia University, 351 Engineering Terrace, Mail Code 8904, 1210 Amsterdam Avenue, New York, New York 10027, USA
    J Acoust Soc Am 130:3059-67. 2011
    ..e., physical mechanism) of the focused ultrasound-induced BBB opening with monodispersed microbubbles is identified...
  34. pmc Acoustically-active microbubbles conjugated to liposomes: characterization of a proposed drug delivery vehicle
    Azadeh Kheirolomoom
    University of California, Davis, Department of Biomedical Engineering, Davis, CA 95616, USA
    J Control Release 118:275-84. 2007
    A new acoustically-active delivery vehicle was developed by conjugating liposomes and microbubbles, using the high affinity interaction between avidin and biotin...
  35. ncbi Role of acoustic cavitation in the delivery and monitoring of cancer treatment by high-intensity focused ultrasound (HIFU)
    C C Coussios
    Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford, UK
    Int J Hyperthermia 23:105-20. 2007
    ..The role of temperature elevation in mitigating bubble-enhanced heating effects is also discussed, along with other bubble-field effects such as multiple scattering and shielding...
  36. ncbi Microbubbles and ultrasound: from diagnosis to therapy
    P A Dijkmans
    Department of Cardiology, Institute for Cardiovascular Research, VU University Medical Center, Amsterdam, The Netherlands
    Eur J Echocardiogr 5:245-56. 2004
    The development of ultrasound contrast agents, containing encapsulated microbubbles, has increased the possibilities for diagnostic imaging...
  37. ncbi Efficient siRNA delivery using novel siRNA-loaded Bubble liposomes and ultrasound
    Yoko Endo-Takahashi
    Department of Drug Delivery and Biopharmaceutics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan
    Int J Pharm 422:504-9. 2012
    ..A specific gene-silencing effect was also achieved by transfection with si-BLs. Thus, the combination of si-BLs with US exposure can be used for delivery of siRNA to a specific tissue via systemic injection...
  38. pmc Targeted multifunctional multimodal protein-shell microspheres as cancer imaging contrast agents
    Renu John
    Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana Champaign, 405 North Mathews Avenue, Urbana, IL 61801, USA
    Mol Imaging Biol 14:17-24. 2012
    ..The development, characterization, and use of multifunctional multimodal microspheres are described for targeted contrast and therapeutic applications...
  39. ncbi Preparation of targeted microbubbles: ultrasound contrast agents for molecular imaging
    Alexander L Klibanov
    Cardiovascular Division, University of Virginia, Charlottesville, VA, 22908 0158, USA
    Med Biol Eng Comput 47:875-82. 2009
    ..can be prepared by attaching targeting ligands to the lipid, protein or polymer shell coating of gas-filled microbubbles. These materials are stable on storage, fully biocompatible and can be administered parenterally...
  40. ncbi Microbubbles as ultrasound contrast agents for molecular imaging: preparation and application
    Sunil Unnikrishnan
    Cardiovascular Division, Cardiovascular Research Center, and Department of Biomedical Engineering, University of Virginia, MR4 RM3146, 409 Lane Rd, PO Box 800158, Charlottesville, VA 22098, USA
    AJR Am J Roentgenol 199:292-9. 2012
    ..The purpose of this review is to describe trends in microbubble application in molecular imaging...
  41. ncbi Augmentation of AAV-mediated cardiac gene transfer after systemic administration in adult rats
    O J Müller
    Department of Cardiology, Internal Medicine III, University Hospital Heidelberg, Heidelberg, Germany
    Gene Ther 15:1558-65. 2008
    ..To analyze efficiency and specificity of gene transfer, microbubbles loaded with AAV-6 or -9 harboring a luciferase or enhanced green fluorescent protein (EGFP) reporter gene were ..
  42. ncbi Ultrasound-targeted microbubble destruction enhances AAV-mediated gene transfection in human RPE cells in vitro and rat retina in vivo
    H L Li
    Department of Ultrasound, Shanghai Jiaotong University Affiliated First People s Hospital, Shanghai, China
    Gene Ther 16:1146-53. 2009
    ..Fluorescence expression was mainly located in the retinal layer. UTMD is a promising method for gene delivery to the retina...
  43. pmc Multi-modality safety assessment of blood-brain barrier opening using focused ultrasound and definity microbubbles: a short-term study
    Babak Baseri
    Department of Biomedical Engineering, Columbia University, New York, NY, USA
    Ultrasound Med Biol 36:1445-59. 2010
    ..Forty-nine mice were intravenously injected with Definity microbubbles (0.05 microL/kg) and sonicated under the following parameters: frequency of 1...
  44. ncbi Nonviral delivery of siRNA into mesenchymal stem cells by a combination of ultrasound and microbubbles
    Kentaro Otani
    Department of Regenerative Medicine and Tissue Engineering, National Cardiovascular Center Research Institute, Suita, Osaka, Japan
    J Control Release 133:146-53. 2009
    ..The aim of this study was to elucidate the feasibility of a combination of ultrasound and microbubbles (US-MB) for delivery of small interfering RNA (siRNA) into mesenchymal stem cells (MSC)...
  45. ncbi Sonodynamic therapy
    Katsuro Tachibana
    Department of Anatomy, Fukuoka University School of Medicine, 7 45 1 Nanakuma, Jonan, Fukuoka 814 0180, Japan
    Ultrasonics 48:253-9. 2008
    ..Furthermore, the introduction of microbubbles and nanobubbles as carriers/enhancers of drugs has added a whole new dimension to therapeutic ultrasound...
  46. ncbi Ultrasound microbubble contrast agents for diagnostic and therapeutic applications: current status and future design
    Shih Tsung Kang
    Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, Taiwan
    Chang Gung Med J 35:125-39. 2012
    Ultrasound contrast agents are highly echogenic microbubbles with many unique properties. Microbubbles can basically improve the sensitivity of conventional ultrasound imaging to the microcirculation...
  47. ncbi Molecular imaging of vulnerable plaques in rabbits using contrast-enhanced ultrasound targeting to vascular endothelial growth factor receptor-2
    Hong Liu
    Department of Ultrasound, Second Affiliated Hospital, The Third Military Medical University, Chongqing 400037, PR China
    J Clin Ultrasound 39:83-90. 2011
    ..We investigated the relationship between retention of a vascular endothelial growth factor receptor 2 (VEGFR-2) targeted UCA and VEGFR-2 expression in a vulnerable plaque model in rabbits...
  48. pmc Renal ultrafiltration changes induced by focused US
    Krisztina Fischer
    Department of Radiology, Brigham and Women s Hospital, Harvard Medical School, Focused Ultrasound Laboratory, 221 Longwood Ave, Room 515, Boston, MA 02215, USA
    Radiology 253:697-705. 2009
    ..To determine if focused ultrasonography (US) combined with a diagnostic microbubble-based US contrast agent can be used to modulate glomerular ultrafiltration and size selectivity...
  49. pmc Improving sensitivity in ultrasound molecular imaging by tailoring contrast agent size distribution: in vivo studies
    Jason E Streeter
    Joint Department of Biomedical Engineering, University of North Carolina North Carolina State University, Chapel Hill, NC, USA
    Mol Imaging 9:87-95. 2010
    ..Prior studies have shown that only small quantities of microbubbles are retained at their target sites, therefore, enhancing contrast sensitivity to low concentrations of ..
  50. ncbi Anti-angiogenic gene therapy for hepatocellular carcinoma mediated by microbubble-enhanced ultrasound exposure: an in vivo experimental study
    Fang Nie
    Department of Medical Ultrasonics, Institution of Diagnostic and Interventional Ultrasound, The First Affiliated Hospital, Sun Yat Sen University, Guangzhou, People s Republic of China
    J Drug Target 16:389-95. 2008
    ..The aim of this study was to assess the effect of anti-angiogenic gene therapy for hepatocelluar carcinoma (HCC) treated by microbubble-enhanced ultrasound exposure...
  51. ncbi Targeted tissue transfection with ultrasound destruction of plasmid-bearing cationic microbubbles
    Jonathan P Christiansen
    Cardiovascular Division, University of Virginia School of Medicine, Charlottesville, VA, USA
    Ultrasound Med Biol 29:1759-67. 2003
    ..the relative efficacy and mechanism of gene transfection by ultrasound (US) destruction of plasmid-bearing microbubbles. Luciferase reporter plasmid was charge-coupled to cationic lipid microbubbles...
  52. ncbi Therapeutic arteriogenesis by ultrasound-mediated VEGF165 plasmid gene delivery to chronically ischemic skeletal muscle
    Howard Leong-Poi
    Division of Cardiology, Keenan Research Centre in the Li Ka Shing Knowledge Institute, St Michael s Hospital, Toronto, Ontario, Canada
    Circ Res 101:295-303. 2007
    ..has been developed using ultrasound-mediated destruction of intravenously administered DNA-bearing carrier microbubbles during their microcirculatory transit...
  53. ncbi Augmentation of cardiac protein delivery using ultrasound targeted microbubble destruction
    Raffi Bekeredjian
    Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, NB 11 200, Dallas, TX 75390 8573, USA
    Ultrasound Med Biol 31:687-91. 2005
    Gas-filled microbubbles have become an important tool as ultrasonic contrast agents. We have previously shown that ultrasound-targeted microbubble destruction (UTMD) can direct plasmids to the heart...
  54. ncbi Long lived microbubbles for oxygen delivery
    Frederic Gerber
    Systemes Organises Fluores a Finalites Therapeutiques SOFFT, Institut Charles Sadron, Strasbourg Cedex, France
    Artif Cells Blood Substit Immobil Biotechnol 35:119-24. 2007
    Exceptionally long lived microbubbles containing a fluorocarbon as part of their filling gas have been obtained by using a fluorinated phospholipid instead of a standard phospholipid as shell component...
  55. ncbi Cavitation-enhanced extravasation for drug delivery
    Costas D Arvanitis
    Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Headington, Oxford, UK
    Ultrasound Med Biol 37:1838-52. 2011
    ....
  56. pmc Intramembrane cavitation as a unifying mechanism for ultrasound-induced bioeffects
    Boris Krasovitski
    Faculty of Biomedical Engineering, Technion Israel Institute of Technology, Haifa 32000, Israel
    Proc Natl Acad Sci U S A 108:3258-63. 2011
    ..8f - 0.5) and is intensified by proximity to free surfaces, the presence of nearby microbubbles in free medium, and the flexibility of the surrounding tissue...
  57. ncbi Injectable microbubbles as contrast agents for diagnostic ultrasound imaging: the key role of perfluorochemicals
    Ernest G Schutt
    Research and Development Department, Alliance Pharmaceutical Corp, 6175 Lusk Blvd, San Diego, CA 92121, USA
    Angew Chem Int Ed Engl 42:3218-35. 2003
    ..Use of fluorocarbon vapor, by extending the persistence of microbubbles in vivo from seconds to minutes, propelled contrast ultrasonography into clinical practice...
  58. ncbi Superparamagnetic nanoparticle-inclusion microbubbles for ultrasound contrast agents
    Fang Yang
    State Key Laboratory of Bioelectronics, Jiangsu Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, Peoples Republic of China
    Phys Med Biol 53:6129-41. 2008
    ..The newly developed US contrast agent microbubbles have a mean diameter of 760 nm with a polydisperity index (PI) of 0.699...
  59. pmc A method for assessing the microvasculature in a murine tumor model using contrast-enhanced ultrasonography
    Mary E Loveless
    Institute of Imaging Science, Vanderbilt University, Nashville, TN 37232 2675, USA
    J Ultrasound Med 27:1699-709. 2008
    ..The purpose of this study was to develop a method for assessing tumor vascularity in a preclinical model of breast cancer using contrast-enhanced ultrasonography...
  60. pmc A novel nested liposome drug delivery vehicle capable of ultrasound triggered release of its payload
    Stuart Ibsen
    Department of Bioengineering, Moores Cancer Center, University of California San Diego, La Jolla, USA
    J Control Release 155:358-66. 2011
    ..This ability to open the outer membrane in small regions of deep tissue could provide a second level of spatial and temporal control beyond biochemical targeting, making these particles promising for in vivo animal studies...
  61. ncbi Enhancement of vascular permeability with low-frequency contrast-enhanced ultrasound in the chorioallantoic membrane model
    Susanne M Stieger
    Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, CA 95616, USA
    Radiology 243:112-21. 2007
    ....
  62. ncbi Ultrasonic imaging of tumor angiogenesis using contrast microbubbles targeted via the tumor-binding peptide arginine-arginine-leucine
    Gregory E R Weller
    Department of Bioengineering and McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Cancer Res 65:533-9. 2005
    ..We hypothesized that ultrasound contrast microbubbles (MB) targeted via linkage with RRL would specifically adhere to tumor angiogenic endothelium versus normal ..
  63. ncbi Iron oxide nanoparticle-containing microbubble composites as contrast agents for MR and ultrasound dual-modality imaging
    Zhe Liu
    Department of Experimental Molecular Imaging ExMI, Helmholtz Institute for Biomedical Engineering, Medical Faculty, RWTH Aachen University, Aachen 52074, Germany
    Biomaterials 32:6155-63. 2011
    ..In this study, iron oxide nanoparticle-embedded polymeric microbubbles were designed as multi-modal contrast agents for hybrid MR-US imaging...
  64. ncbi Use of ultrasound contrast agents for gene or drug delivery in cardiovascular medicine
    Raffi Bekeredjian
    Department of Internal Medicine, Division of Cardiology, University of Texas Southwestern Medical Center, Dallas, TX, USA
    J Am Coll Cardiol 45:329-35. 2005
    ..We discuss the potential applications and limitations of this technique and suggest future applications in cardiovascular medicine...
  65. pmc Effect of microbubble size on fundamental mode high frequency ultrasound imaging in mice
    Shashank Sirsi
    Department of Chemical Engineering, Columbia University, New York, NY 10027, USA
    Ultrasound Med Biol 36:935-48. 2010
    ..To study the effect of individual microbubble size populations, microbubbles of 1-2 microm, 4-5 microm and 6-8 microm diameter were isolated using the technique of differential ..
  66. ncbi Progress in the development of ultrasound-mediated gene delivery systems utilizing nano- and microbubbles
    Ryo Suzuki
    Department of Biopharmaceutics, School of Pharmaceutical Sciences, Teikyo University, 1091 1 Suwarashi, Midori ku, Sagamihara, Kanagawa 252 5195, Japan
    J Control Release 149:36-41. 2011
    Recently, ultrasound-mediated gene delivery with nano- and microbubbles was developed as a novel non-viral vector system...
  67. pmc US imaging of tumor angiogenesis with microbubbles targeted to vascular endothelial growth factor receptor type 2 in mice
    Jurgen K Willmann
    Department of Radiology and Bio X Program, Stanford University School of Medicine, James H Clark Center, 318 Campus Dr, East Wing, 1st Floor, Stanford, CA 94305 5427, USA
    Radiology 246:508-18. 2008
    To prospectively evaluate contrast material-enhanced ultrasonography (US) with microbubbles targeted to vascular endothelial growth factor receptor type 2 (VEGFR2) for imaging tumor angiogenesis in two murine tumor models.
  68. pmc Ultrasound radiation force enables targeted deposition of model drug carriers loaded on microbubbles
    Aaron F H Lum
    University of California Davis, Department of Biomedical Engineering, 451 East Health Sciences Drive, Davis, CA 95616, USA
    J Control Release 111:128-34. 2006
    ..consist of avidinated fluorescent nanobeads bound directly to the biotinylated lipid shells of preformed microbubbles. USRF was used to deflect the vehicle from the center of flow to a tube surface in order to facilitate receptor-..
  69. pmc Design and evaluation of doxorubicin-containing microbubbles for ultrasound-triggered doxorubicin delivery: cytotoxicity and mechanisms involved
    Ine Lentacker
    Laboratory of General Biochemistry and Physical Pharmacy, Department of Pharmaceutics, Ghent University, Ghent, Belgium
    Mol Ther 18:101-8. 2010
    Drug delivery with microbubbles and ultrasound is gaining more and more attention in the drug delivery field due to its noninvasiveness, local applicability, and proven safety in ultrasonic imaging techniques...
  70. ncbi Sonoporation by ultrasound-activated microbubble contrast agents: effect of acoustic exposure parameters on cell membrane permeability and cell viability
    Raffi Karshafian
    Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada
    Ultrasound Med Biol 35:847-60. 2009
    ..the effect of ultrasound exposure parameters on the sonoporation of KHT-C cells in suspension by perflutren microbubbles. Variations in insonating acoustic pressure (0.05 to 3.5 MPa), pulse frequency (0.5 to 5...
  71. pmc Assessment of molecular imaging of angiogenesis with three-dimensional ultrasonography
    Jason E Streeter
    Joint Department of Biomedical Engineering, University of North Carolina North Carolina State University, Chapel Hill, NC 27599, USA
    Mol Imaging 10:460-8. 2011
    ..3D MI, including volumetric analysis of inter- and intra-animal targeting, provides a thorough way of characterizing angiogenesis and will be a more robust assessment technique for the future of MI...
  72. pmc A triple-targeted ultrasound contrast agent provides improved localization to tumor vasculature
    Jason M Warram
    University of Alabama at Birmingham, G082 Volker Hall, 1670 University Blvd, Birmingham, AL 35294 USA
    J Ultrasound Med 30:921-31. 2011
    ..This report summarizes an evaluation of multitargeted microbubbles, comparing single-, dual-, and triple-targeted motifs.
  73. ncbi Microbubble contrast agents: targeted ultrasound imaging and ultrasound-assisted drug-delivery applications
    Alexander L Klibanov
    Department of Internal Medicine, Cardiovascular Division, University of Virginia, Charlottesville, Virginia 22908 0158, USA
    Invest Radiol 41:354-62. 2006
    ..b>Microbubbles as contrast materials require a small dosage and show excellent detection sensitivity...
  74. ncbi Effective gene delivery with novel liposomal bubbles and ultrasonic destruction technology
    Ryo Suzuki
    Department of Biopharmaceutics, School of Pharmaceutical Sciences, Teikyo University, 1091 1 Suwarashi, Sagamiko, Sagamihara, Kanagawa 229 0195, Japan
    Int J Pharm 354:49-55. 2008
    ..Previously, it was reported that microbubbles, utilized as imaging agents for diagnostic echocardiography, could promote gene delivery into cells when ..
  75. ncbi Superparamagnetic iron oxide nanoparticle-embedded encapsulated microbubbles as dual contrast agents of magnetic resonance and ultrasound imaging
    Fang Yang
    Jiangsu Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
    Biomaterials 30:3882-90. 2009
    ..The in vivo MRI experiments also show that the SPIO-inclusion microbubbles have longer contrast-enhancement duration time in rat liver than non-SPIO-inclusion microbubbles...
  76. ncbi Microbubbles in medical imaging: current applications and future directions
    Jonathan R Lindner
    Cardiovascular Division, University of Virginia Medical Center, Charlottesville, Virginia 22908, USA
    Nat Rev Drug Discov 3:527-32. 2004
  77. ncbi Ultrasound contrast agents: an overview
    David Cosgrove
    Imaging Sciences Department, Imperial College, Hammersmith Hospital, London, United Kingdom
    Eur J Radiol 60:324-30. 2006
    ..Underpinning this development is the fact that gases are compressible, and thus the microbubbles expand and contract in the alternating pressure waves of the ultrasound beam, while tissue is almost ..
  78. ncbi Vibrating microbubbles poking individual cells: drug transfer into cells via sonoporation
    Annemieke van Wamel
    Department of Experimental Echocardiography, Thoraxcentre, Erasmus MC, Rotterdam, The Netherlands
    J Control Release 112:149-55. 2006
    Ultrasound contrast microbubbles have the ability to enhance endothelial cell permeability and thus may be used as a new way to deliver drugs...
  79. pmc Targeted microbubbles for imaging tumor angiogenesis: assessment of whole-body biodistribution with dynamic micro-PET in mice
    Jurgen K Willmann
    Molecular Imaging Program at Stanford, Department of Radiology and Bio X Program, Stanford University School of Medicine, James H Clark Center, 318 Campus Dr, East Wing, 1st Floor, Stanford, CA 94305 5427, USA
    Radiology 249:212-9. 2008
    To evaluate in vivo whole-body biodistribution of microbubbles (MBs) targeted to tumor angiogenesis-related vascular endothelial growth factor (VEGF) receptor 2 (VEGFR2) by using dynamic micro-positron emission tomography (PET) in living ..
  80. ncbi Microbubble-enhanced ultrasound to deliver an antisense oligodeoxynucleotide targeting the human androgen receptor into prostate tumours
    Petra Haag
    Department of Urology, Innsbruck Medical University, A 6020 Innsbruck, Anichstrasse 35, Austria
    J Steroid Biochem Mol Biol 102:103-13. 2006
    ..Our short antisense AR ODNs were loaded onto the lipid surface of cationic gas-filled microbubbles by ion charge binding, and delivered into the cells by bursting the loaded microbubbles with ultrasound...
  81. ncbi Selective laser nano-thermolysis of human leukemia cells with microbubbles generated around clusters of gold nanoparticles
    Dmitri O Lapotko
    Luikov Heat and Mass Transfer Institute, Minsk, 220072, Belarus
    Lasers Surg Med 38:631-42. 2006
    ..The objective of this study was two-fold: to optimize NP targeting to real tumor (human) cells and to better understand physical mechanisms of cell damage for improved control of the laser ablation...
  82. ncbi Assessment of tissue perfusion by contrast-enhanced ultrasound
    Emilio Quaia
    Department of Radiology, Cattinara Hospital, University of Trieste, Strada di Fiume 447, Trieste, 34149, Italy
    Eur Radiol 21:604-15. 2011
    ..presents several advantages over other imaging techniques in assessing tissue perfusion, including the use of microbubbles as blood-pool agents, portability, availability and absence of exposure to radiation or nuclear tracers...
  83. pmc Advances in molecular imaging with ultrasound
    Ryan Gessner
    Joint Department of Biomedical Engineering, University of North Carolina North Carolina State University, Chapel Hill, NC, USA
    Mol Imaging 9:117-27. 2010
    ....
  84. pmc Efficient gene delivery to pancreatic islets with ultrasonic microbubble destruction technology
    Shuyuan Chen
    Department of Internal Medicine, Cardiology Section, Baylor University Medical Center, Baylor Heart and Vascular Institute, 621 North Hall Street, Suite H030, Dallas, TX 75226, USA
    Proc Natl Acad Sci U S A 103:8469-74. 2006
    ..The technique involves incorporation of plasmids into the phospholipid shell of gas-filled microbubbles, which are then infused into rats and destroyed within the pancreatic microcirculation with ultrasound...
  85. pmc Ultrasound-mediated gene delivery with cationic versus neutral microbubbles: effect of DNA and microbubble dose on in vivo transfection efficiency
    Cedric M Panje
    Department of Radiology, Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford, California, USA
    Theranostics 2:1078-91. 2012
    ..To assess the effect of varying microbubble (MB) and DNA doses on the overall and comparative efficiencies of ultrasound (US)-mediated gene delivery (UMGD) to murine hindlimb skeletal muscle using cationic versus neutral MBs...
  86. ncbi Characterization of acoustic properties of PVA-shelled ultrasound contrast agents: ultrasound-induced fracture (part II)
    Dmitry Grishenkov
    Marcus Wallenberg Laboratory, Royal Institute of Technology, Teknikringen 8, Stockholm 100 44, Sweden
    Ultrasound Med Biol 35:1139-47. 2009
    ..of the peak negative pressure, P(thr), at which ultrasound contrast agents fracture is relevant for using these microbubbles both as devices for contrast enhancement purposes, as well as carriers of drugs to be delivered locally...
  87. ncbi Ultrasound, microbubbles and the blood-brain barrier
    Stephen Meairs
    Department of Neurology, Universitatsklinikum Mannheim, University of Heidelberg, 68167 Mannheim, Germany
    Prog Biophys Mol Biol 93:354-62. 2007
    ..If microbubbles are combined with ultrasound exposure, the effects of ultrasound can be focused upon the vasculature to reduce ..
  88. ncbi Phospholipid-stabilized microbubble foam for injectable oxygen delivery
    Edward J Swanson
    Department of Chemical Engineering, Columbia University, New York, New York 10027, USA
    Langmuir 26:15726-9. 2010
    A detailed study is presented on the synthesis and characterization of purely oxygen-filled microbubbles (OMBs) stabilized by phospholipids...
  89. pmc Controlled and targeted tumor chemotherapy by ultrasound-activated nanoemulsions/microbubbles
    Natalya Y Rapoport
    Department of Bioengineering, University of Utah, Salt Lake City, UT 84112, USA
    J Control Release 138:268-76. 2009
    ..of ovarian, breast, and pancreatic cancerous tumors by paclitaxel-loaded nanoemulsions that convert into microbubbles locally in tumor tissue under the action of tumor-directed therapeutic ultrasound...
  90. pmc Examination of inertial cavitation of Optison in producing sonoporation of chinese hamster ovary cells
    Monica M Forbes
    Department of Bioengineering, University of Illinois at Urbana Champaign, Urbana, IL, USA
    Ultrasound Med Biol 34:2009-18. 2008
    ....
  91. pmc Sonoporation enhances chemotherapeutic efficacy in retinoblastoma cells in vitro
    Nahyoung G Lee
    Doheny Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA
    Invest Ophthalmol Vis Sci 52:3868-73. 2011
    To study the ability of ultrasound (US) and microbubbles (MB) to enhance chemotherapeutic efficacy against retinoblastoma Y79 cells in vitro.
  92. ncbi Ultrasonic imaging of molecular targets
    Georg Schmitz
    Department for Electrical Engineering and Information Technology, Ruhr Universitat Bochum, Universitatsstrasse 150, 44780, Bochum, Germany
    Basic Res Cardiol 103:174-81. 2008
    ..Ultrasound-contrast-enhanced imaging strategies make use of the specific physical properties of microbubbles such as resonance, nonlinear oscillation, and collapse...
  93. ncbi Adeno-associated virus vector delivery to the heart
    Lawrence T Bish
    Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    Methods Mol Biol 807:219-37. 2011
    ..a protocol for vector delivery into the left ventricular anterior wall by ultrasound-targeted destruction of microbubbles loaded with AAV...
  94. pmc Blood-brain barrier: real-time feedback-controlled focused ultrasound disruption by using an acoustic emissions-based controller
    Meaghan A O'Reilly
    Physical Science Platform, Sunnybrook Research Institute, Toronto, ON, Canada M4N 3M5
    Radiology 263:96-106. 2012
    To determine if focused ultrasound disruption of the blood-brain barrier (BBB) can be safely controlled by using real-time modulation of treatment pressures on the basis of acoustic emissions from the exposed microbubbles.
  95. ncbi Concurrent blood-brain barrier opening and local drug delivery using drug-carrying microbubbles and focused ultrasound for brain glioma treatment
    Chien Yu Ting
    Biomedical Engineering and Environmental Science, National Tsing Hua University, Hsinchu 30013, Taiwan, ROC
    Biomaterials 33:704-12. 2012
    ..3%-39.6%) and improved median survival (29 days-32.5 days). This study provides a new approach for designing multifunctional MBs to facilitate FUS-mediated chemotherapy for brain tumor treatment...
  96. ncbi Studies on neutral, cationic and biotinylated cationic microbubbles in enhancing ultrasound-mediated gene delivery in vitro and in vivo
    Nikolitsa Nomikou
    Sonidel Ltd, Tonlegee Drive, Dublin 5, Ireland
    Acta Biomater 8:1273-80. 2012
    ..of facilitating targeted gene expression and is significantly enhanced in the presence of exogenously added microbubbles. This study explores the influence of microbubble surface modifications on their interaction with plasmid DNA ..
  97. ncbi A novel microbubble construct for intracardiac or intravascular MR manometry: a theoretical study
    Rohan Dharmakumar
    Department of Medical Biophysics, Sunnybrook and Women s College Health Sciences Centre, University of Toronto, Toronto, ON M4N 3M5, Canada
    Phys Med Biol 50:4745-62. 2005
    ..It was recently reported that such an MR-based pressure measurement with microbubbles at 1...
  98. ncbi Correlation between estimates of tumor perfusion from microbubble contrast-enhanced sonography and dynamic contrast-enhanced magnetic resonance imaging
    Thomas E Yankeelov
    Institute of Imaging Science, Department of Radiology, Vanderbilt University, Nashville, Tennessee, USA
    J Ultrasound Med 25:487-97. 2006
    ..We compared measurements of tumor perfusion from microbubble contrast-enhanced sonography (MCES) and dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) in an animal tumor model...
  99. ncbi Quantitative and qualitative investigation into the impact of focused ultrasound with microbubbles on the triggered release of nanoparticles from vasculature in mouse tumors
    Chung Yin Lin
    Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan Division of Medical Engineering Research, National Health Research Institutes, Miaoli, Taiwan
    J Control Release 146:291-8. 2010
    ....
  100. ncbi Controlled release of Fe3O4 nanoparticles in encapsulated microbubbles to tumor cells via sonoporation and associated cellular bioeffects
    Fang Yang
    Jiangsu Key Laboratory for Biomaterials and Devices, Nanjing, 210009, China
    Small 7:902-10. 2011
    Fe(3)O(4) nanoparticles embedded in the shells of encapsulated microbubbles could be used therapeutically as in situ drug-delivery vehicles...
  101. ncbi Angiogenesis imaging--ultrasound
    D Cosgrove
    Imaging Sciences Department, Imperial College, Hammersmith Hospital, London, UK
    Br J Radiol 76:S43-9. 2003

Research Grants70

  1. Development of an In Vivo Stem Cell Tracking Method Using Ultrasound Imaging
    Flordeliza S Villanueva; Fiscal Year: 2010
    ..Acoustically active gas-filled polymer microbubbles will be developed as contrast agents for ex vivo labeling of MSCs prior to therapeutic delivery...
  2. NIR Light-Activated Nanoparticles for Drug and Gene Delivery
    JOSEPH ANTHONY ZASADZINSKI; Fiscal Year: 2013
    ..by converting the physiologically friendly NIR light energy absorbed by the HGN to heat, creating unstable microbubbles that mechanically rupture endosomes and release siRNA to the cytosol within seconds...
  3. Brain Tumor-Penetrating Nanoparticle Delivery with MR-Guided Focused Ultrasound
    Richard J Price; Fiscal Year: 2013
    ..approach that couples magnetic resonance (MR)-targeted BBB opening via focused ultrasound (FUS) and microbubbles (MBs) with drug-loaded nanoparticles that have been engineered with extremely dense polyethylene glycol (PEG) ..
  4. Detection of Brown Adipose Tissue Using Contrast Ultrasound
    Marielle Scherrer-Crosbie; Fiscal Year: 2012
    ..Contrast-enhanced ultrasound involves the intravenous infusion of echogenic microbubbles during the ultrasound procedure, allowing the estimation of tissue blood flow...
  5. Transcranial Sonothrombolysis with Diagnostic Ultrasound
    Thilo Hoelscher; Fiscal Year: 2013
    ..thrombolysis, how to optimize the US settings for effective clot lysis in intracranial arteries and how microbubbles accelerate sonothrombolysis are poorly understood...
  6. Ultrasound-Assisted AQP1 Gene Therapy for Functional Restoration of Salivary Glan
    Michael J Passineau; Fiscal Year: 2013
    ..transient, non-invasive, non-viral gene transfer to the salivary gland using "off the shelf" FDA-approved microbubbles and clinical ultrasonography equipment...
  7. Molecular Imaging of Ischemic Memory with Ultrasound - Transition to Humans
    Jonathan R Lindner; Fiscal Year: 2010
    ..agent that is suitable for human use that is produced by conjugating a recombinant dimeric PSGL-1 analogue to microbubbles. The analogue alone is safe and is being tested in Phase-2 studies for amelioration of organ transplant injury...
  8. Ultrasound-activated microbubbles for targeted siRNA delivery to tumor
    Flordeliza S Villanueva; Fiscal Year: 2013
    ....
  9. IVUS Detection of Rupture Prone Plaques
    Marvin M Doyley; Fiscal Year: 2013
    ..The conjugated microbubbles will be visualized by employing the second harmonic contrast imaging mode, which we propose to implement on a ..
  10. Biomechanics of Leukocytes and Molecular Imaging Agents
    MICHAEL BRANDEAU LAWRENCE; Fiscal Year: 2010
    ..Antibodies to P-selectin, alphaVbetaS integrin, and targeting peptides were conjugated to lipid-shelled microbubbles and successfully applied to image tumor angiogenesis and inflammation of the kidneys, heart, and other tissues ..
  11. Enzyme-Responsive Nanoemulsions as Tumor-Specific Ultrasound Contrast Agents for
    Andrew P Goodwin; Fiscal Year: 2011
    ..Sadik Esener on developing stimulus-responsive polymer-coated perfluorocarbon microbubbles as ultrasound contrast agents that activate only in regions of inflammation...
  12. Ultrasound mediated targeted therapy for kidney disease
    Joshua J Rychak; Fiscal Year: 2010
    ..Antibodies against two targets will be investigated: P-selectin and TGF-beta. Antibody-bearing microbubbles will be administered intravenously and accumulate at sites of intravascular expression of TGF-beta and P-..
  13. Quantification and monitoring inflammation in IBD with Molecular Ultrasound
    JUERGEN KARL WILLMANN; Fiscal Year: 2013
    ..of US with those of molecular imaging and develop a molecular US imaging strategy using novel contrast microbubbles that allow inflammation imaging at the molecular level...
  14. Enzyme-Responsive Nanoemulsions as Tumor-Specific Ultrasound Contrast Agents
    Andrew P Goodwin; Fiscal Year: 2013
    ..Sadik Esener on developing stimulus-responsive polymer-coated perfluorocarbon microbubbles as ultrasound contrast agents that activate only in regions of inflammation...
  15. Early Detection of Pancreatic Cancer with Targeted Contrast-enhanced Ultrasound
    JUERGEN KARL WILLMANN; Fiscal Year: 2010
    ..We will use novel, clinically translatable microbubbles targeted at human vascular endothelial growth factor receptor type 2 (KDR), which has been shown to be ..
  16. Targeted theranostic microbubble vectors for transcription factor decoy delivery
    Flordeliza S Villanueva; Fiscal Year: 2013
    ..transcription factor decoys that capitalizes on unique bioeffects ensuing from ultrasound-induced vibrations of microbubbles (MBs)...
  17. Cardiovascular Inflammation Function Imaging by Photoacoustics using Gold Nanorod
    Kang Kim; Fiscal Year: 2010
    ..While current molecular imaging systems such as PET/CT, MRI, and contrast-enhanced ultrasound imaging using microbubbles have been applied to cardiovascular atherosclerosis imaging with limited success, an imaging technique based on ..
  18. Image-guided Ultrasound-triggered Tumor Therapy
    Alexander L Klibanov; Fiscal Year: 2011
    ..Drug carrier microbubbles and liposome constructs will be applied to release antitumor agents or thrombus-generating enzyme into tumor ..
  19. COMBINED ULTRASOUND/MOLECULAR ANALYSIS OF TUMOR RESPONSE TO VEGF BLOCKADE
    Jessica J Kandel; Fiscal Year: 2010
    ..Yet this technology is still in its infancy, and further development of long-circulating and targeted microbubbles is critical for realizing its full potential as a means of evaluating dynamic changes in vessel structure and ..
  20. Precision engineering of ultrasonically-targeted drug delivery vehicles
    Paul A Dayton; Fiscal Year: 2012
    ..the University of California Irvine provide a unique and qualified research group with expertise in ultrasound, microbubbles, drug delivery vehicles and their drug loading and release characteristics, and microfluidics required to ..
  21. Microbubble Infused Hydrogels for Cartilage Tissue Engineering
    Clark T Hung; Fiscal Year: 2012
    ..In this potentially high-reward R21 proposal, we explore the novel application of lipid-shelled, gas-filled microbubbles (used clinically as ultrasound contrast agents) as a method for creating cell laden microporous hydogels for ..
  22. Cavitation Enhancement of Biospecimen processing for Improved DNA Fragmentation
    WILLIAM PERRY JANZEN; Fiscal Year: 2013
    ..We propose to apply the use of lipid encapsulated microbubbles to the fragmentation of DNA from both purified genomic and formaldehyde crosslinked samples...
  23. Ultrasound-Targeted Therapy for Acute Myocardial Infarction
    Sanjiv Kaul; Fiscal Year: 2013
    ..3. Integrate the therapy probe(s) with a 4D ultrasound imaging system that can image microbubbles in vivo and gate ultrasound pulses to automatically dissolve the thrombus. 4...
  24. Ultrasound-Assisted Gene Transfer of Anti-Biofilm Peptides to the Salivary Gland
    Michael J Passineau; Fiscal Year: 2012
    ..This method utilizes low frequency ultrasound pulses to destroy microbubbles, causing local cavitation and opening transient pores in cell membranes enabling non-viral vectors to enter ..
  25. Ultrasound-triggered delivery of siRNA as treatment for diabetic kidney disease
    Joshua J Rychak; Fiscal Year: 2012
    ..application of high-power ultrasound energy specifically to the kidneys, which causes rapid destruction of the microbubbles and a transient poration of the adjacent cells...
  26. Ultrasound Contrast Agents for Inflammation and Angiogenesis
    Joshua J Rychak; Fiscal Year: 2010
    Site-targeted microbubbles (MB) have demonstrated potential as a molecular imaging tool. A new targeted MB platform was developed in phase I of this project...
  27. Ultrasound-Mediated Gene Therapy for Hemophilia B
    CAROL MIAO; Fiscal Year: 2009
    ..We have shown that ultrasound (US) in the presence of microbubbles [MBs] enhances gene delivery of a liver-specific, high-expressing factor IX (FIX) plasmid into mice...
  28. Ultrasound-Mediated Gene Therapy for Hemophilia B
    Carol H Miao; Fiscal Year: 2012
    ..We have shown that ultrasound (US) in the presence of microbubbles [MBs] enhances gene delivery of a liver-specific, high-expressing factor IX (FIX) plasmid into mice...
  29. Bevacizumab Delivery to Glioblastoma with MR-Guided Focused Ultrasound
    Richard J Price; Fiscal Year: 2013
    ..FUS) will be applied to MR-targeted GBMs following the intravenous administration of ultrasound contrast agent microbubbles (MBs)...
  30. Molecular Ultrasound for Early Breast Cancer Detection
    JUERGEN KARL WILLMANN; Fiscal Year: 2013
    ..of ultrasound with the advantages of molecular imaging through use of molecularly targeted ultrasound contrast microbubbles. This modified targeted contrast-enhanced ultrasound imaging strategy (molecular ultrasound imaging), may ..
  31. Fluorescence Lifetime Imaging Microscope
    David J Hall; Fiscal Year: 2013
    ..monitoring protein-protein interactions, nanoparticle accumulation or cleavage, FRET-quenching of fluorescent microbubbles, caspase-8 kinetics, and neutrophil adhesion in sickle cell disease...
  32. Therapeutic Window for Acute Stroke Therapy (P50)
    Brett C Meyer; Fiscal Year: 2012
    ..to study the potential of diagnostic ultrasound in combination with rt-PA and/or ultrasound contrast agent microbubbles on image-guided transcranial thrombolysis;(3) Stroke Team Remote Evaluation Using a Digital Observation Camera -..
  33. Novel RTK Targeting Strategies in Glioblastoma
    Roger Abounader; Fiscal Year: 2013
    ..the systemic and local deliveries of RTK ligands and microRNAs to GBM xenografts using focused ultrasound and microbubbles/nanoparticles as well as convection enhanced delivery...
  34. Ultrasound-triggered drug delivery with acoustically active red blood cells
    Alexander L Klibanov; Fiscal Year: 2013
    ..Ultrasound can image and non-invasively deliver therapy. Microbubbles are intravascular ultrasound contrast agents that provide blood pool imaging in real time and targeted (..
  35. Molecular Targeted, Focused, Ultrasound-Based Delivery of Antiproliferative Drugs
    John A Hossack; Fiscal Year: 2012
    ..modalities and reagents to employ ultrasound to direct the focal release of an anti-proliferative agent from microbubbles to a blood vessel following angioplasty-induced stenosis under ultrasound imaging guidance...
  36. Therapeutic Microlesioning by Contrast Echocardiography for Myocardial Reduction
    Oliver D Kripfgans; Fiscal Year: 2013
    ..MCE) has enabled visualization of myocardial perfusion by imaging strong echos from contrast-agent microbubbles. For high amplitude MCE, intermittent destruction of contrast microbubbles has been shown to lethally injure ..
  37. Specific and high-resolution ultrasound cancer imaging
    Katherine W Ferrara; Fiscal Year: 2013
    ..Now, we demonstrate that in vivo insonation of targeted microbubbles creates biological effects using parameters that were previously found to be safe with a non-targeted agent;in ..
  38. Vascular endoluminal cellular paving using acoustic radiation force
    Catalin Toma; Fiscal Year: 2013
    ..This effect is particularly strong with gas-filled lipid microbubbles used as ultrasound contrast agents...
  39. Nanotherapeutics for acute kidney injury
    MARK DOUGLAS OKUSA; Fiscal Year: 2013
    ..Current delivery systems use microbubbles that consist of a gas core encapsulated by a shell (several nm thick) with drugs embedded within the membrane ..
  40. Enhancing Delivery and Regulating Gene Expression in Oncolytic Vaccinia Vectors
    Stephen H Thorne; Fiscal Year: 2013
    ..or epitope removal will be used to reduce antibody recognition, (ii) binding of viral particles to targeted microbubbles, followed by ultrasound mediated release within the tumor environment and (iii) use of cell carriers to deliver ..
  41. Contrast agents for high-sensitivity ultrasound molecular imaging of tumor angiog
    Joshua J Rychak; Fiscal Year: 2011
    ..Gas-encapsulated particles known as microbubbles (MB) are used as contrast agents for ultrasound, and recent research has identified MB diameter as the key ..
  42. Ultrasound-guided gene delivery in pancreatic cancer
    Pier Paolo Claudio; Fiscal Year: 2010
    ..By using US contrast agents (microbubbles) we will achieve both specificity of action as well as protection of the viral vectors from inactivation...
  43. ULTRASOUND THERAPY DELIVERY THROUGH INTACT SKULL
    Kullervo Hynynen; Fiscal Year: 2013
    ..period, we furthered our initial research and developed methods to enhance FUS interaction with tissue using microbubbles, thus making whole brain sonications feasible...
  44. Sonolysis in Acute Coronary Syndromes
    Feng Xie; Fiscal Year: 2012
    ..Transcutaneous high mechanical index (MI) ultrasound and intravenous microbubbles have successfully recanalized peripheral vessel thromboses in animal studies...
  45. Ultrasound guided site-specific gene delivery in prostate cancer
    Pier Paolo Claudio; Fiscal Year: 2010
    ..The impact of microbubbles on gene therapy is to protect the viral vectors from rapid degradation by the immune system thus allowing for ..
  46. Histotripsy Tissue Interactions for BPH Therapy
    William W Roberts; Fiscal Year: 2013
    ..Each ultrasound pulse creates a localized, highly dynamic cluster of microbubbles (a bubble cloud) that oscillates and violently collapses...
  47. New Tools for Prostate Cancer Detection and Prognostication
    Sanjiv S Gambhir; Fiscal Year: 2013
    ..In project #2 we propose the adaptation of our latest ultrasound technology using tumor angiogenesis-targeted microbubbles to image prostate cancer...
  48. PACIFIC OVARIAN CANCER RESEARCH CONSORTIUM
    NICOLE DENISE URBAN; Fiscal Year: 2013
    ..in high-risk women using serum markers validated in preclinical samples, a novel imaging trial using targeted microbubbles to improve ultrasound performance, validation of a microRNA plasma signature for respectability, exploration of ..
  49. Isolation of rare circulating tumor cells from blood using microbubbles
    DIMITRI SIMBERG; Fiscal Year: 2010
    ..We propose to use targeted microbubbles (hereafter [unreadable]bubbles) to develop a method for isolation and analysis of rare tumor cells...
  50. High quality CTC isolation using microbubbles for downstream molecular analysis
    Yu Tsueng Liu; Fiscal Year: 2013
    ..Lipid shell-perfluorocarbon buoyant microbubbles (MBs) coated with anti-EpCAM antibody immediately attached to tumor cells in unfractionated blood, and isolated ..
  51. Dosimetry of Cavitation Microlesions in Microbubble Enhanced Medical Ultrasound
    DOUGLAS LAWRENCE MILLER; Fiscal Year: 2013
    ..Stabilized microbubbles circulate with the blood and are activated by ultrasound pulses to yield microbubble-specific echos...
  52. Development of Theranostic Ultrasound
    Thomas R Porter; Fiscal Year: 2013
    ..utilized in small animal models to efficiently enhance thrombolysis in the presence of intravenously infused microbubbles. These high acoustic pressures induce inertial cavitation (IC) of the microbubbles, which may also cause ..
  53. Histotripsy for Benign Prostatic Hyperplasia
    William W Roberts; Fiscal Year: 2010
    ..by cavitation, a phenomenon where rapid cycling from compression to rarefaction results in formation of microbubbles within the tissue...
  54. Optimization of ultrasound-facilitated blood-brain barrier opening
    Elisa E Konofagou; Fiscal Year: 2012
    ..Of the methods used for BBB disruption shown to be effective, Focused Ultrasound (FUS), in conjunction with microbubbles, is the only technique that can induce localized BBB opening noninvasively and regionally...
  55. ECHOCARDIOGRAPHIC ASSESSMENT OF MYOCARDIAL PERFUSION
    Sanjiv Kaul; Fiscal Year: 2004
    ..It used gas-filled microbubbles as perfusion tracers, which scatter ultrasound during their transit through any vascular compartment...
  56. ECHOCARDIOGRAPHIC ASSESSMENT OF MYOCARDIAL PERFUSION
    Sanjiv Kaul; Fiscal Year: 2002
    ..It used gas-filled microbubbles as perfusion tracers, which scatter ultrasound during their transit through any vascular compartment...
  57. Ultrasound-enabled two-photon FRET microscopy
    JEROME C MERTZ; Fiscal Year: 2012
    ..two types of samples: genetically encoded FRET probes in tissue (or tissue-like environments), and labeled microbubbles in solution. In the former case, we will investigate the application of our technique to in-vivo imaging in C...
  58. Stimulus-Responsive Microbubbles for Site-Specific Imaging of Thrombosis
    Robert F Mattrey; Fiscal Year: 2012
    ..The resultant microbubble can be detected via its harmonic oscillations with excellent specificity. Microbubbles will be designed to exhibit sensitivity to thrombin, a protease involved in the coagulation cascade...
  59. Enhancing ultra-sound-mediated tumor ablation with phase-shift nanoemulation
    Tyrone Porter; Fiscal Year: 2013
    ..Enhancing ultrasound-mediated tumor ablation with phase-shift nanoemulsion It is well documented that microbubbles can enhance the absorption of ultrasound in tissue...
  60. Dynamics of Vascular Microbubbles and Microdroplets in Gas Embolotherapy
    Joseph L Bull; Fiscal Year: 2013
    ..regions of healthy tissue for occlusion, we are ready to apply this knowledge to study the potential for lodged microbubbles to induce bioeffects that may lead to localized hypoxia and tissue necrosis...
  61. In Vivo Analysis of Stationary Gas Filled Hollow Iron Doped Silica Nanoshells
    Alexander Liberman; Fiscal Year: 2013
    ..to develop a technique to employ preoperative injection of ultra- sound contrast enhancement stationary microbubbles and micro/[nano]shells to enable intraoperative detection of tumors even in cases with small skin incisions to ..
  62. Understanding the Biophysics of Ultrasound-Induced Angiogenesis
    CHENARA ALEXIS JOHNSON; Fiscal Year: 2012
    ..The assumed process, for studies involving contrast agent, is inertial cavitation, however microbubbles can also cause microstreaming and liquid jets...
  63. CONFERENCE ON GENOMIC INSTABILITY AND DYSREGULATION
    Charles Fox; Fiscal Year: 1991
    ..Growth of a short bolus of encapsulated microbubbles will also be investigated. Creation, growth and maintenance of bubbles will be evaluated extensively in water...
  64. ECHOCARDIOGRAPHIC ASSESSMENT OF MICROVASCULAR PERFUSION
    Kevin Wei; Fiscal Year: 2003
    ..I. with a basic understanding of ultrasound physics, coronary physiology and perfusion imaging. MCE uses microbubbles as a contrast agent during simultaneous ultrasound imaging, and has recently developed into a unique tool for ..
  65. BIOMECHANICS OF LEUKOCYTE ADHESION MOLECULES
    Klaus Ley; Fiscal Year: 2002
    ..We will use the insights gained to design liposome-based targeted drug delivery systems and ultrasound contrast microbubbles for delivery in the vascular system under shear flow...
  66. Shared Instrumentation: Vevo 770 Ultrasound System
    David Pollock; Fiscal Year: 2009
    ..varied and include ECHO cardiography, vascular wall structural imaging, pulse Doppler flow measurements, and microbubbles for quantification of intrarenal blood flow and tracking biomarkers of vascular inflammation...
  67. ECHOCARDIOGRAPHY OF MYOCARDIAL PERFUSION/FUNCTION
    Sanjiv Kaul; Fiscal Year: 1991
    ..It is hoped that this research will provide an in-depth understanding of the kinetics of microbubbles through the myocardium at different coronary flow rates and the effect of flow on the microbubbles...
  68. US with Submicron Bubbles for Sentinel Node Detection
    Erik Wisner; Fiscal Year: 2004
    ..and that submicron and near micron-sized bubbles appear to be more effective than conventional intravascular microbubbles for this purpose...
  69. Ultrasound contrast agent dynamics and vascular effects
    THOMAS MATULA; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Ultrasound contrast agent microbubbles are composed of a variety of gases and stabilizing shell materials...
  70. Bubble Based Acoustic Force Elasticity for Presbyopia
    KYLE HOLLMAN; Fiscal Year: 2006
    ..measure tissue elasticity by deforming it with acoustic radiation force applied to femtosecond laser produced microbubbles. First we will study physical functional relationships by measuring bubble motion in gelatin phantoms with ..