lasers

Summary

Summary: Light Amplification by Stimulated Emission of Radiation. This phenomenon is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the visible, infrared, or ultraviolet regions of the spectrum. They are capable of producing immense heat and power when focused at close range and are used in surgical procedures, in diagnosis, and in physiologic studies. Lasers used at low power, transmitting light not heat, have been used in wound healing and pain control (LASER THERAPY, LOW-LEVEL).

Top Publications

  1. pmc Megabase chromatin domains involved in DNA double-strand breaks in vivo
    E P Rogakou
    Laboratory of Molecular Pharmacology, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Cell Biol 146:905-16. 1999
  2. pmc PARP inhibition versus PARP-1 silencing: different outcomes in terms of single-strand break repair and radiation susceptibility
    Camille Godon
    Institut Curie, Centre de Recherche INSERM, U612, Institut Curie, Bât 110 112, Centre Universitaire, F 91405 Orsay, France
    Nucleic Acids Res 36:4454-64. 2008
  3. ncbi Laser-capture microdissection
    Virginia Espina
    Center for Applied Proteomics and Molecular Medicine, George Mason University, 10900 University Blvd MS 4E3, Manassas, Virginia, USA
    Nat Protoc 1:586-603. 2006
  4. pmc Far-red fluorescent protein excitable with red lasers for flow cytometry and superresolution STED nanoscopy
    Kateryna S Morozova
    Biophys J 99:L13-5. 2010
  5. pmc Various spatiotemporal expression profiles of anther-expressed genes in rice
    Tokunori Hobo
    Bioscience and Biotechnology Center, Nagoya University, Furocho, Chikusa, Nagoya, 464 8601 Japan
    Plant Cell Physiol 49:1417-28. 2008
  6. ncbi Mechanisms of pulsed laser ablation of biological tissues
    Alfred Vogel
    Medical Laser Center Lübeck, Peter Monnik Weg 4, D 23562 Lubeck, Germany
    Chem Rev 103:577-644. 2003
  7. ncbi Effect of controlled laser microporation on drug transport kinetics into and across the skin
    Y G Bachhav
    School of Pharmaceutical Sciences, University of Geneva, 30 Quai Ernest Ansermet, 1211 Geneva 4, Switzerland
    J Control Release 146:31-6. 2010
  8. ncbi Using laser microporation to improve transdermal delivery of diclofenac: Increasing bioavailability and the range of therapeutic applications
    Yogeshwar G Bachhav
    School of Pharmaceutical Sciences, University of Geneva and University of Lausanne, Geneva, Switzerland
    Eur J Pharm Biopharm 78:408-14. 2011
  9. pmc Separated transcriptomes of male gametophyte and tapetum in rice: validity of a laser microdissection (LM) microarray
    Keita Suwabe
    Graduate School of Life Sciences, Tohoku University, Sendai, 980 8577 Japan
    Plant Cell Physiol 49:1407-16. 2008
  10. ncbi A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage
    T T Paull
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0540, USA
    Curr Biol 10:886-95. 2000

Detail Information

Publications324 found, 100 shown here

  1. pmc Megabase chromatin domains involved in DNA double-strand breaks in vivo
    E P Rogakou
    Laboratory of Molecular Pharmacology, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Cell Biol 146:905-16. 1999
    ..The results further suggest the possible existence of units of higher order chromatin structure involved in monitoring DNA integrity...
  2. pmc PARP inhibition versus PARP-1 silencing: different outcomes in terms of single-strand break repair and radiation susceptibility
    Camille Godon
    Institut Curie, Centre de Recherche INSERM, U612, Institut Curie, Bât 110 112, Centre Universitaire, F 91405 Orsay, France
    Nucleic Acids Res 36:4454-64. 2008
    ..PARP-1 inhibition and deletion thus have different outcomes in terms of SSBR and radiosensitivity...
  3. ncbi Laser-capture microdissection
    Virginia Espina
    Center for Applied Proteomics and Molecular Medicine, George Mason University, 10900 University Blvd MS 4E3, Manassas, Virginia, USA
    Nat Protoc 1:586-603. 2006
    ..Herein we provide a thorough description of LCM techniques, with an emphasis on tips and troubleshooting advice derived from LCM users. The total time required to carry out this protocol is typically 1-1.5 h...
  4. pmc Far-red fluorescent protein excitable with red lasers for flow cytometry and superresolution STED nanoscopy
    Kateryna S Morozova
    Biophys J 99:L13-5. 2010
    ..imaging and multicolor labeling in the red wavelength range, as well as probes excitable with standard red lasers in flow cytometry and fluorescence microscopy...
  5. pmc Various spatiotemporal expression profiles of anther-expressed genes in rice
    Tokunori Hobo
    Bioscience and Biotechnology Center, Nagoya University, Furocho, Chikusa, Nagoya, 464 8601 Japan
    Plant Cell Physiol 49:1417-28. 2008
    ....
  6. ncbi Mechanisms of pulsed laser ablation of biological tissues
    Alfred Vogel
    Medical Laser Center Lübeck, Peter Monnik Weg 4, D 23562 Lubeck, Germany
    Chem Rev 103:577-644. 2003
  7. ncbi Effect of controlled laser microporation on drug transport kinetics into and across the skin
    Y G Bachhav
    School of Pharmaceutical Sciences, University of Geneva, 30 Quai Ernest Ansermet, 1211 Geneva 4, Switzerland
    J Control Release 146:31-6. 2010
    ..L.E.A.S.E.(R) (i) to create well-defined conduits in the skin, (ii) to provide a controlled enhancement of transdermal transport and (iii) to enable improvement in both the rate and extent of drug delivery...
  8. ncbi Using laser microporation to improve transdermal delivery of diclofenac: Increasing bioavailability and the range of therapeutic applications
    Yogeshwar G Bachhav
    School of Pharmaceutical Sciences, University of Geneva and University of Lausanne, Geneva, Switzerland
    Eur J Pharm Biopharm 78:408-14. 2011
    ..A similar improvement in topical diclofenac delivery in vivo may increase the number of potential therapeutic applications...
  9. pmc Separated transcriptomes of male gametophyte and tapetum in rice: validity of a laser microdissection (LM) microarray
    Keita Suwabe
    Graduate School of Life Sciences, Tohoku University, Sendai, 980 8577 Japan
    Plant Cell Physiol 49:1407-16. 2008
    ..These results indicate that the 44K LM-microarray is a reliable tool to analyze the gene expression profiles of two important cell types in the anther, the microspore/pollen and tapetum...
  10. ncbi A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage
    T T Paull
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0540, USA
    Curr Biol 10:886-95. 2000
    ..Recently it has been reported that a member of the histone H2A family, H2AX, becomes extensively phosphorylated within 1-3 minutes of DNA damage and forms foci at break sites...
  11. ncbi X-ray lasers for structural and dynamic biology
    J C H Spence
    Department of Physics, Arizona State University, Tempe, AZ 85287, USA
    Rep Prog Phys 75:102601. 2012
    Research opportunities and techniques are reviewed for the application of hard x-ray pulsed free-electron lasers (XFEL) to structural biology...
  12. ncbi Targeted transfection by femtosecond laser
    Uday K Tirlapur
    Laser Microscopy, Institute of Anatomy II, Friedrich Schiller University, Jena, Germany
    Nature 418:290-1. 2002
    ..Not only does this superior optical technique give high transfection efficiency and cell survival, but it also allows simultaneous evaluation of the integration and expression of the introduced gene...
  13. pmc Ex vivo dynamic imaging of retinal microglia using time-lapse confocal microscopy
    Jung Eun Lee
    Office of the Scientific Director, National Eye Institute, National Institutes of Health, Bethesda, Maryland, USA
    Invest Ophthalmol Vis Sci 49:4169-76. 2008
    ..Here, the authors used a novel ex vivo retinal imaging preparation to examine the behavioral phenotype of living retinal microglia in intact tissue and in response to injury...
  14. ncbi Extended coherence length Fourier domain mode locked lasers at 1310 nm
    Desmond C Adler
    Lightlab Imaging Inc, a St Jude Medical subsidiary, 1 Technology Park Drive, Westford, MA 01886, USA
    Opt Express 19:20930-9. 2011
    Fourier domain mode locked (FDML) lasers are excellent tunable laser sources for frequency domain optical coherence tomography (FD-OCT) systems due to their combination of high sweep rates, large tuning ranges, and high output powers...
  15. ncbi Nociceptive laser-evoked brain potentials do not reflect nociceptive-specific neural activity
    A Mouraux
    Department of Clinical Neurology, University of Oxford, OX1 3QX Oxford, United Kingdom
    J Neurophysiol 101:3258-69. 2009
    ....
  16. pmc Gene discovery and annotation using LCM-454 transcriptome sequencing
    Scott J Emrich
    Bioinformatics and Computational Biology Graduate Program, Iowa State University, Ames, IA 50010, USA
    Genome Res 17:69-73. 2007
    ..We conclude that the coupling of LCM and 454 sequencing technologies facilitates the discovery of rare, possibly cell-type-specific transcripts...
  17. pmc Optical stimulation of the facial nerve: a new monitoring technique?
    Ingo Ulrik Teudt
    Department of Otolaryngology Head and Neck Surgery, Northwestern University, Feinberg School of Medicine, Chicago, Illinois 60611 3008, USA
    Laryngoscope 117:1641-7. 2007
    ..In contrast to electrical stimulation, pulsed infrared optical radiation can be used to safely and selectively stimulate neural tissue and might be valuable for screening...
  18. pmc Determinants of laser-evoked EEG responses: pain perception or stimulus saliency?
    G D Iannetti
    Department of Physiology, Anatomy, and Genetics, University of Oxford, South Parks Road, OX1 3QX, Oxford, UK
    J Neurophysiol 100:815-28. 2008
    ..e., its ability to capture attention). Therefore laser-evoked EEG responses represent an indirect readout of the function of the nociceptive system...
  19. pmc Selective cell targeting with light-absorbing microparticles and nanoparticles
    Costas M Pitsillides
    Wellman Laboratories of Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
    Biophys J 84:4023-32. 2003
    ..Thus they may be particularly useful for prelabeling cells in engineered tissue before implantation. Subsequent irradiation with laser pulses will allow control of the implanted cells (inactivation or modulation) in a noninvasive manner...
  20. ncbi Targeted microinjection into cells and retina using optoporation
    Ling Gu
    University of Texas at Arlington, Department of Physics, Biophysics and Physiology Lab, Arlington, Texas 76019, USA
    J Biomed Opt 16:128003. 2011
    ..Laser assisted delivery of exogenous material into a specific area of three-dimensional neuronal tissue, such as the retina, will help to understand the functioning of neuronal circuitry of normal and degenerated retina...
  21. ncbi Specific cell targeting with nanobody conjugated branched gold nanoparticles for photothermal therapy
    Bieke Van de Broek
    IMEC, Bio Nano Electronics, Functional Nanosystems, Kapeldreef 75, B 3001 Leuven, Belgium
    ACS Nano 5:4319-28. 2011
    ..These results suggest that this new type of bioconjugated branched gold nanoparticles are effective antigen-targeted photothermal therapeutic agents for cancer treatment...
  22. pmc The brain effects of laser acupuncture in healthy individuals: an FMRI investigation
    Im Quah-Smith
    School of Psychiatry, Faculty of Medicine, University of New South Wales, Randwick, New South Wales, Australia
    PLoS ONE 5:e12619. 2010
    ....
  23. pmc Role of ocular complement factor H in a murine model of choroidal neovascularization
    Valeriy V Lyzogubov
    Department of Ophthalmology, Jones Eye Institute, Pat and Willard Walker Eye Research Center, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    Am J Pathol 177:1870-80. 2010
    ..In conclusion, our findings provide evidence that CFH present on RPE and choroid regulates local MAC formation that is critical for the development of laser-induced CNV...
  24. pmc Optical cochlear implants: evaluation of surgical approach and laser parameters in cats
    Suhrud M Rajguru
    Department of Otolaryngology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611 3008, USA
    Hear Res 269:102-11. 2010
    ..Combined with histological data, the results suggest that pulsed IR stimulation does not lead to detectable acute tissue damage and validate the stimulation parameters that can be used in future chronic implants based on pulsed IR...
  25. ncbi In vivo confocal imaging of the retina in animal models using scanning laser ophthalmoscopy
    Mathias W Seeliger
    Retinal Diagnostics Research Group, Tuebingen, Germany
    Vision Res 45:3512-9. 2005
    Scanning-laser ophthalmoscopy is a technique for confocal imaging of the eye in vivo. The use of lasers of different wavelengths allows to obtain information about specific tissues and layers due to their reflection and transmission ..
  26. ncbi Application of dynamic diffractive optics for enhanced femtosecond laser based cell transfection
    Maciej Antkowiak
    SULSA, School of Biology, University of St Andrews, St Andrews KY169TS, UK
    J Biophotonics 3:696-705. 2010
    ..Additionally, fluorescent imaging is used to demonstrate cell selective two-step gene therapy...
  27. ncbi Application of infrared laser to the zebrafish vascular system: gene induction, tracing, and ablation of single endothelial cells
    Eiji Kimura
    Department of Anatomy, Iwate Medical University, Iwate, Japan
    Arterioscler Thromb Vasc Biol 33:1264-70. 2013
    ..We applied this method to the vascular system in zebrafish embryos and demonstrated its usability to investigate mechanisms of vascular morphogenesis in vivo...
  28. ncbi The impact of treatment density and molecular weight for fractional laser-assisted drug delivery
    Christina S Haak
    Department of Dermatology, Bispebjerg Hospital, University of Copenhagen, Bispebjerg Bakke 23, 2400 Copenhagen NV, Denmark
    J Control Release 163:335-41. 2012
    Ablative fractional lasers (AFXL) facilitate uptake of topically applied drugs by creating narrow open micro-channels into the skin, but there is limited information on optimal laser settings for delivery of specific molecules...
  29. pmc Recruitment and retention dynamics of RECQL5 at DNA double strand break sites
    Venkateswarlu Popuri
    Laboratory of Molecular Gerontology, National Institute on Aging, NIH, 251 Bayview Blvd, Suite 100, Baltimore, MD 21224, USA
    DNA Repair (Amst) 11:624-35. 2012
    ..Also, the recruitment of RECQL5 to laser-induced damage sites is independent of the presence of other DNA damage signaling and repair proteins BLM, WRN and ATM...
  30. ncbi High-throughput optical injection of mammalian cells using a Bessel light beam
    Helen A Rendall
    SUPA School of Physics and Astronomy, University of St Andrews, Scotland KY16 9SS
    Lab Chip 12:4816-20. 2012
    ..Using this method, we have achieved viable injection of HL60 cells with propidium iodide with an efficiency of 20.4 ± 4.2% and CHO-K1 cells (31.0 ± 9.5%) at a rate of up to 10 cells s(-1)...
  31. pmc Transcutaneous immunotherapy via laser-generated micropores efficiently alleviates allergic asthma in Phl p 5-sensitized mice
    D Bach
    Division of Allergy and Immunology, Department of Molecular Biology, University of Salzburg, Salzburg, Austria
    Allergy 67:1365-74. 2012
    ..The current study was performed to compare the efficacy of transcutaneous immunotherapy via laser-generated micropores to subcutaneous injection...
  32. ncbi Habituation to pain in "medication overuse headache": a CO2 laser-evoked potential study
    Diana Ferraro
    Department of Neurosciences, Catholic University, Rome, Italy
    Headache 52:792-807. 2012
    ....
  33. pmc Infrared photostimulation of the crista ampullaris
    Suhrud M Rajguru
    Department of Otolaryngology, Northwestern University, Chicago, IL 60611, USA
    J Physiol 589:1283-94. 2011
    ..The present data support the hypothesis that pulsed IR activates sensory hair cells, thus leading to modulation of synaptic transmission and afferent nerve discharge reported here...
  34. pmc Femtosecond X-ray protein nanocrystallography
    Henry N Chapman
    Center for Free Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
    Nature 470:73-7. 2011
    ..This offers a new approach to structure determination of macromolecules that do not yield crystals of sufficient size for studies using conventional radiation sources or are particularly sensitive to radiation damage...
  35. ncbi Influence of laser parameters on nanoparticle-induced membrane permeabilization
    Cuiping Yao
    Xi an Jiaotong University, School of Life Science and Technology, Institute of Biomedical Analytical Technology and Instrumentation, Xianning xi Road 28, Xi an, 710049, China
    J Biomed Opt 14:054034. 2009
    ..These results suggest that the laser-induced permeabilization approach constitutes a promising tool for targeted delivery of larger exogenous molecules into living cells...
  36. pmc Acoustic events and "optophonic" cochlear responses induced by pulsed near-infrared laser
    Ingo Ulrik Teudt
    Institute of Audioneurotechnology, Hannover School of Medicine, Hannover, Germany
    IEEE Trans Biomed Eng 58:1648-55. 2011
    ..Most optical stimulation was performed with pulsed lasers operating with near-infrared (NIR) light and in thermal confinement...
  37. ncbi Light interactions with gold nanorods and cells: implications for photothermal nanotherapeutics
    Constantin Ungureanu
    Biomedical Photonic Imaging Group, MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P O Box 217, 7500 AE Enschede, The Netherlands
    Nano Lett 11:1887-94. 2011
    ..Both effects create sufficiently high optical absorption at 532 nm, which otherwise would have been present at the LP peak. We discuss implications of these finding on the application of these particles in biomedicine...
  38. ncbi Femtosecond laser-assisted microinjection into living neurons
    Ming Lei
    State Key Laboratory of Transient Optics and Photonics, Xi an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi an 710119, China
    J Neurosci Methods 174:215-8. 2008
    ..The transfection efficiency reached almost 100%. This method also had the advantage of contact-free, non-disruptive and stable transfection...
  39. pmc Laser-capture microdissection: refining estimates of the quantity and distribution of latent herpes simplex virus 1 and varicella-zoster virus DNA in human trigeminal Ganglia at the single-cell level
    Kening Wang
    Medical Virology Section, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892, USA
    J Virol 79:14079-87. 2005
    ..These data indicate that both HSV-1 and VZV DNAs persist in human TG primarily, if not exclusively, in a moderate percentage of neuronal cells...
  40. ncbi Sensitivity and specificity of multiphoton laser tomography for in vivo and ex vivo diagnosis of malignant melanoma
    Enrico Dimitrow
    Department of Dermatology and Allergology, University of Jena, Jena, Germany
    J Invest Dermatol 129:1752-8. 2009
    ..By means of this procedure accuracy values up to 97% were reached. These findings underline the potential applicability of multiphoton laser tomography in melanoma diagnosis of melanocytic skin lesions...
  41. ncbi Laser speckle flowmetry for the study of cerebrovascular physiology in normal and ischemic mouse cortex
    Cenk Ayata
    Stroke and Neurovascular Regulation Laboratory, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129, USA
    J Cereb Blood Flow Metab 24:744-55. 2004
    ..In summary, LSF has distinct advantages over LDF for CBF monitoring because of high spatial resolution...
  42. pmc Hematopoietic stem cells provide repair functions after laser-induced Bruch's membrane rupture model of choroidal neovascularization
    Tailoi Chan-Ling
    Department of Anatomy and Histology, Institute for Biomedical Research, University of Sydney, Sydney, Australia
    Am J Pathol 168:1031-44. 2006
    ..These studies suggest that HSCs could serve as a therapeutic source for long-term regeneration of injured retina and choroid in diseases such as age-related macular degeneration and retinitis pigmentosa...
  43. ncbi Quantum dot microdrop laser
    J Schafer
    Institute of Optics, Information and Photonics, Max Planck Research Group, University of Erlangen Nuremberg, Erlangen, Germany
    Nano Lett 8:1709-12. 2008
    ..Substantial laser signals at low thresholds are measured from the directional emission normal to the pump beam, owing to the high Q cavity modes...
  44. pmc Cytoplasmic molecular delivery with shock waves: importance of impulse
    T Kodama
    Wellman Laboratories of Photomedicine, Massachusetts General Hospital, and Department of Dermatology, Harvard Medical School, Boston, MA 02114, USA
    Biophys J 79:1821-32. 2000
    ..The duration of the pressure pulse of the shock tube was 100 times longer than the lasers. The uptake of two fluorophores, calcein (molecular weight: 622) and fluorescein isothiocyanate-dextran (molecular ..
  45. ncbi Confocal laser endomicroscopy: technical status and current indications
    A Hoffman
    First Dept of Medicine, Johannes Gutenberg University, Mainz, Germany
    Endoscopy 38:1275-83. 2006
    ..Molecular imaging is becoming feasible, and this will shortly open the door to new indications in gastrointestinal endoscopy (e.g., in vivo receptor analysis)...
  46. pmc Large production system for hyperpolarized 129Xe for human lung imaging studies
    F WILLIAM HERSMAN
    Department of Physics, University of New Hampshire, 131 Main Street, Nesmith Hall, Durham, NH 03824, USA
    Acad Radiol 15:683-92. 2008
    ..Human lung imaging studies with xenon, initially limited by the modest quantity and quality of hyperpolarized gas available, can now be performed with multiliter quantities several times daily...
  47. pmc Two-photon single-cell optogenetic control of neuronal activity by sculpted light
    Bertalan K Andrasfalvy
    Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, VA 20147, USA
    Proc Natl Acad Sci U S A 107:11981-6. 2010
    ..The demonstrated spatiotemporal resolution and the selectivity provided by TEFO allow manipulation of neuronal activity, with a large number of applications in studies of neuronal microcircuit function in vitro and in vivo...
  48. pmc Pulsed laser versus electrical energy for peripheral nerve stimulation
    Jonathon Wells
    Vanderbilt University, Department of Biomedical Engineering, 5824 Stevenson Center, Nashville, TN 37235, United States
    J Neurosci Methods 163:326-37. 2007
    ..Thus, use of a pulsed laser exhibits distinct advantages when compared to standard electrical means for excitation of muscle potentials in the peripheral nerve in the research domain and possibly for clinical diagnostics in the future...
  49. ncbi Comparison of the bond strength of laser-sintered and cast base metal dental alloys to porcelain
    Tolga Akova
    Department of Prosthodontics, Faculty of Dentistry, Cukurova University 01330 Balcali, Adana, Turkey
    Dent Mater 24:1400-4. 2008
    ..The purpose of this study was to compare shear bond strengths of cast Ni-Cr and Co-Cr alloys and the laser-sintered Co-Cr alloy to dental porcelain...
  50. pmc Albumin therapy improves local vascular dynamics in a rat model of primary microvascular thrombosis: a two-photon laser-scanning microscopy study
    Anitha Nimmagadda
    Cerebral Vascular Disease Research Center, Department of Neurology D4 5, University of Miami Miller School of Medicine, PO Box 016960, Miami, FL 33101, USA
    Stroke 39:198-204. 2008
    ..To explore the hypothesis that albumin's protective effect is mediated in part by salutary intravascular mechanisms, we assessed microvascular hemodynamics in a model of laser-induced cortical arteriolar thrombosis...
  51. pmc Role of cortical tension in bleb growth
    Jean Yves Tinevez
    Max Planck Institute of Molecular Cell Biology and Genetics, 01 307 Dresden, Germany
    Proc Natl Acad Sci U S A 106:18581-6. 2009
    ..Together, our observations and model allow us to relate bleb shape parameters to the underlying cellular mechanics and provide insights as to how bleb formation can be biochemically regulated during cell motility...
  52. ncbi Modulation of pain ratings by expectation and uncertainty: Behavioral characteristics and anticipatory neural correlates
    Christopher A Brown
    Human Pain Research Group, Clinical Sciences Building, Hope Hospital, Salford M6 8HD, United Kingdom
    Pain 135:240-50. 2008
    ..This suggests that biasing of pain reports and LEPs by expectation involves temporally precise activity in specific cortical networks...
  53. pmc Intravenous transferrin, RGD peptide and dual-targeted nanoparticles enhance anti-VEGF intraceptor gene delivery to laser-induced CNV
    S R Singh
    Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE, USA
    Gene Ther 16:645-59. 2009
    ..Thus, surface-functionalized nanoparticles allow targeted gene delivery to the neovascular eye on intravenous administration and inhibit the progression of laser-induced CNV in a rodent model...
  54. ncbi MPScope: a versatile software suite for multiphoton microscopy
    Quoc Thang Nguyen
    Physics Department, University of California, San Diego, CA 92093 7697, USA
    J Neurosci Methods 156:351-9. 2006
    ..The combination of optimized code, multithreading and COM (Common Object Model) technologies enables MPScope to fully take advantage of custom-built two-photon microscopes and to simplify their realization...
  55. pmc The polarity and dynamics of microtubule assembly in the budding yeast Saccharomyces cerevisiae
    P S Maddox
    Department of Biology, CB3280, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    Nat Cell Biol 2:36-41. 2000
    ..These results show for astral and anaphase interpolar spindle microtubules, and possibly for metaphase spindle microtubules, that microtubule assembly and disassembly occur at plus, and not minus, ends...
  56. ncbi Chromophore-assisted laser inactivation in cell biology
    Ken Jacobson
    Department of Cell and Developmental Biology, University of North Carolina Chapel Hill, Chapel Hill, NC 27599 7090, USA
    Trends Cell Biol 18:443-50. 2008
    ..In this review, we examine the applications of CALI to cell biology and discuss the underlying photochemical mechanisms that mediate this powerful technique...
  57. ncbi Clathrate nanostructures for mass spectrometry
    Trent R Northen
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Nature 449:1033-6. 2007
    ....
  58. pmc Tunable plasmonic nanobubbles for cell theranostics
    E Y Lukianova-Hleb
    Laboratory for Laser Cytotechnologies, A V Lykov Heat and Mass Transfer Institute, 15 Brovka Street, Minsk 220072, Belarus
    Nanotechnology 21:85102. 2010
    ..Thus the PNBs acted as tunable theranostic agents at the cellular level and in one process that have supported diagnosis, therapy and guidance of the therapy...
  59. pmc Macromolecular-scale resolution in biological fluorescence microscopy
    Gerald Donnert
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Proc Natl Acad Sci U S A 103:11440-5. 2006
    ..Triplet-state relaxation can be realized either by reducing the repetition rate of pulsed lasers or by increasing the scanning speed such that the build-up of the triplet state is effectively prevented...
  60. ncbi Functional magnetic resonance imaging detects activation of the visual association cortex during laser acupuncture of the foot in humans
    Christian M Siedentopf
    Department of Radiology II, University Hospital of Innsbruck, Innsbruck, Austria
    Neurosci Lett 327:53-6. 2002
    ..These results indicate that fMRI has the potential to elucidate effects of acupuncture on brain activity...
  61. ncbi Stromal gene expression predicts clinical outcome in breast cancer
    Greg Finak
    McGill Centre for Bioinformatics, 3775 University Street, McGill University, Quebec H3A 2B4, Canada
    Nat Med 14:518-27. 2008
    ..Genes represented in the SDPP reveal the strong prognostic capacity of differential immune responses as well as angiogenic and hypoxic responses, highlighting the importance of stromal biology in tumor progression...
  62. pmc Gold nanoparticle mediated laser transfection for efficient siRNA mediated gene knock down
    Dag Heinemann
    Biomedical Optics Department, Laser Zentrum Hannover e V, Hannover, Germany
    PLoS ONE 8:e58604. 2013
    ..Our findings emphasize that gold nanoparticle mediated laser transfection provides an excellent tool for molecular delivery for both, high throughput purposes and the transfection of sensitive cells types...
  63. pmc High precision and fast functional mapping of cortical circuitry through a novel combination of voltage sensitive dye imaging and laser scanning photostimulation
    Xiangmin Xu
    Dept of Anatomy and Neurobiology, School of Medicine, Univ of California, Irvine, CA 92697 1275, USA
    J Neurophysiol 103:2301-12. 2010
    ..Given that this innovation enables rapid mapping and precise evaluation of cortical organization and function, it can have broad impacts in the field of cortical circuitry...
  64. ncbi CD59, a complement regulatory protein, controls choroidal neovascularization in a mouse model of wet-type age-related macular degeneration
    Nalini S Bora
    Department of Ophthalmology, Jones Eye Institute, Pat and Willard Walker Eye Research Center, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
    J Immunol 178:1783-90. 2007
    ..Thus, our results suggest that the inhibition of complement by soluble CD59 may provide a novel therapeutic alternative to current treatment...
  65. ncbi A multi-color fast-switching microfluidic droplet dye laser
    Sindy K Y Tang
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Lab Chip 9:2767-71. 2009
    ..6 kHz-the fastest among all dye lasers reported; it has potential applications in on-chip spectroscopy and flow cytometry.
  66. pmc A role for a CXCR2/phosphatidylinositol 3-kinase gamma signaling axis in acute and chronic vascular permeability
    Julie Gavard
    Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, 30 Convent Drive, Bethesda, MD 20892, USA
    Mol Cell Biol 29:2469-80. 2009
    ..These findings indicate that the CXCR2 and PI3Kgamma signaling pathway may represent a suitable target for the development of novel therapeutic strategies for human diseases characterized by vascular leakage...
  67. pmc Tree height growth measurement with single-scan airborne, static terrestrial and mobile laser scanning
    Yi Lin
    Finnish Geodetic Institute, Geodeetinrinne 2, 02430 Masala, Finland
    Sensors (Basel) 12:12798-813. 2012
    ..Overall, the effectiveness of the proposed technique is primarily validated...
  68. ncbi Infrared laser-mediated gene induction in targeted single cells in vivo
    Yasuhiro Kamei
    Radiation Biology Center, Kyoto University, Yoshida konoe cho, Sakyo ku, Kyoto, Japan
    Nat Methods 6:79-81. 2009
    ....
  69. pmc Phase-sensitive swept-source optical coherence tomography imaging of the human retina with a vertical cavity surface-emitting laser light source
    WooJhon Choi
    Department of Electrical Engineering and Computer Science, and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Opt Lett 38:338-40. 2013
    ..A robust, simple, and computationally efficient phase stabilization approach for phase-sensitive swept-source imaging is also presented...
  70. ncbi X-ray free electron lasers motivate bioanalytical characterization of protein nanocrystals: serial femtosecond crystallography
    Michael J Bogan
    Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California 94025, United States
    Anal Chem 85:3464-71. 2013
    ..This Feature will introduce the concept of serial femtosecond crystallography to the nonexpert, briefly review progress to date, and highlight some potential contributions from the analytical sciences...
  71. ncbi Selective laser nano-thermolysis of human leukemia cells with microbubbles generated around clusters of gold nanoparticles
    Dmitri O Lapotko
    Luikov Heat and Mass Transfer Institute, Minsk, 220072, Belarus
    Lasers Surg Med 38:631-42. 2006
    ..The objective of this study was two-fold: to optimize NP targeting to real tumor (human) cells and to better understand physical mechanisms of cell damage for improved control of the laser ablation...
  72. ncbi Application of heat shock promoter in transgenic zebrafish
    Wataru Shoji
    Department of Cell Biology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan
    Dev Growth Differ 50:401-6. 2008
    ..In this paper, we review the applications of the zebrafish heat shock protein promoter as a gene expression tool and for lineage labeling and transcription enhancer screening...
  73. pmc Neuroprotectin D1 attenuates laser-induced choroidal neovascularization in mouse
    Kristopher G Sheets
    Department of Ophthalmology and the Neuroscience Center of Excellence, Louisiana State University Health Sciences Center, New Orleans, LA, USA
    Mol Vis 16:320-9. 2010
    ..Specifically, this was assessed by clinically grading laser-induced lesions, measuring leakage area, and volumetrically quantifying vascular endothelial cell proliferation...
  74. pmc Plasmonic nanobubbles as transient vapor nanobubbles generated around plasmonic nanoparticles
    Ekaterina Lukianova-Hleb
    A V Lykov Heat and Mass Transfer Institute, 15 P Brovka Street, Minsk 220072, Belarus
    ACS Nano 4:2109-23. 2010
    ..Optical scattering by PNB and its diameter (measured as the lifetime) has been varied with the fluence of laser pulse, and this has demonstrated the tunable nature of PNB...
  75. pmc Highly nonlinear photodamage in two-photon fluorescence microscopy
    A Hopt
    , Department of Membrane Biophysics, D-37077 Goettingen, Germany
    Biophys J 80:2029-36. 2001
    ..5) than that of the two-photon signal (mu approximately 2)...
  76. ncbi Black silicon: substrate for laser 3D micro/nano-polymerization
    Albertas Zukauskas
    Laser Nanophotonics Group, Laser Research Center, Department of Quantum Electronics, Physics Faculty, Vilnius University, Sauletekio 9, LT 10222 Vilnius, Lithuania
    Opt Express 21:6901-9. 2013
    ..Electrical conductivity of substrate and its permeability in liquids are promising for bio- and electroplating applications...
  77. pmc Delivery of molecules into cells using carbon nanoparticles activated by femtosecond laser pulses
    Prerona Chakravarty
    Institute for Bioengineering and Biosciences, Center for Drug Design, Development and Delivery, and Laser Dynamics Laboratory, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
    Nat Nanotechnol 5:607-11. 2010
    ..Our initial results suggest that interaction between the laser energy and carbon black nanoparticles may generate photoacoustic forces by chemical reaction to create transient holes in the membrane for intracellular delivery...
  78. ncbi TRPV4 channels mediate the infrared laser-evoked response in sensory neurons
    E S Albert
    U 1051, INSERM Institut des Neurosciences de Montpellier, Montpellier, France
    J Neurophysiol 107:3227-34. 2012
    ..These results have the potential to facilitate greatly the design of future prosthetic devices aimed at restoring neurosensory capacities in disabled patients...
  79. ncbi Off-resonance plasmonic enhanced femtosecond laser optoporation and transfection of cancer cells
    Judith Baumgart
    Ecole Polytechnique de Montreal, Laser processing and Plasmonics Laboratory, Engineering Physics Department, Montreal, Quebec H3C 3A7, Canada
    Biomaterials 33:2345-50. 2012
    ..This efficient and low toxicity method is a promising alternative to viral transfection for skin cancer treatment...
  80. ncbi Single gallium nitride nanowire lasers
    Justin C Johnson
    Department of Chemistry, University of California, Berkeley, California 94720 1460, USA
    Nat Mater 1:106-10. 2002
    ..much practical interest, because it is widely used in electrically pumped ultraviolet-blue light-emitting diodes, lasers and photodetectors...
  81. pmc Exteroceptive aspects of nociception: insights from graphesthesia and two-point discrimination
    Carsten Dahl Mørch
    Center for Sensory Motor Interaction, Health Science and Technology, Aalborg University, Fredrik Bajersvej 7, D3, DK 9220 Aalborg, Denmark
    Pain 151:45-52. 2010
    ....
  82. ncbi Phototransfection of mammalian cells using femtosecond laser pulses: optimization and applicability to stem cell differentiation
    Patience Mthunzi
    University of St Andrews, School of Physics and Astronomy, North Haugh, St Andrews, Fife, Scotland, United Kingdom
    J Biomed Opt 15:041507. 2010
    ..Extending previous studies [Stevenson et al., Opt. Express 14, 7125-7133 (2006)], we show that femtosecond lasers can be used to phototransfect a range of different cell lines, and specifically that this novel technology can ..
  83. pmc Sensor fusion of monocular cameras and laser rangefinders for line-based Simultaneous Localization and Mapping (SLAM) tasks in autonomous mobile robots
    Xinzheng Zhang
    School of Electrical and Information Engineering, Jinan University, Zhuhai 519070, Guangdong, China
    Sensors (Basel) 12:429-52. 2012
    ..This data association method relaxes the pleonastic computation. The experimental results validate the performance of the proposed sensor fusion and data association method...
  84. pmc Selective gene transfection of individual cells in vitro with plasmonic nanobubbles
    Ekaterina Y Lukianova-Hleb
    Joint American, Belarussian Laboratory for Fundamental and Biomedical Nanophotonics, Rice University, Houston, TX, USA
    J Control Release 152:286-93. 2011
    ..Thus, PNBs provided targeted gene delivery at the single cell level in a single pulse procedure that can be used for safe and effective gene therapy...
  85. ncbi Photo-thermal tumor ablation in mice using near infrared-absorbing nanoparticles
    D Patrick O'Neal
    Nanospectra Biosciences, Inc, 8285 El Rio Suite 130, Houston, TX 77054, USA
    Cancer Lett 209:171-6. 2004
    ..This simple, non-invasive procedure shows great promise as a technique for selective photo-thermal tumor ablation...
  86. ncbi The fit of cobalt-chromium three-unit fixed dental prostheses fabricated with four different techniques: a comparative in vitro study
    Anders Ortorp
    Department Prosthetic Dentistry Dental Materials Science, Institute of Odontology, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
    Dent Mater 27:356-63. 2011
    ..This study sought to evaluate and compare the marginal and internal fit in vitro of three-unit FDPs in Co-Cr made using four fabrication techniques, and to conclude in which area the largest misfit is present...
  87. pmc Topical application of recombinant calreticulin peptide, vasostatin 48, alleviates laser-induced choroidal neovascularization in rats
    Youn Shen Bee
    Department of Ophthalmology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan
    Mol Vis 16:756-67. 2010
    ..Vasostatin 48 (VS48) is a peptide of 48 amino acids derived from calreticulin. This study aimed to investigate the effects of topical application of VS48 eyedrops on experimental choroidal neovascularization (CNV)...
  88. ncbi Patterning and electronic tuning of laser scribed graphene for flexible all-carbon devices
    Veronica Strong
    Department of Chemistry and Biochemistry and California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California 90095, United States
    ACS Nano 6:1395-403. 2012
    ..The LSG also shows exceptional electrochemical activity that surpasses other carbon-based electrodes in electron charge transfer rate as demonstrated using a ferro-/ferricyanide redox couple...
  89. ncbi Plasma mediated off-resonance plasmonic enhanced ultrafast laser-induced nanocavitation
    Etienne Boulais
    Laser processing and Plasmonics Laboratory, Engineering Physics Department, Ecole Polytechnique de Montreal, Montreal, Quebec H3C 3A7, Canada
    Nano Lett 12:4763-9. 2012
    ....
  90. ncbi Photoacoustic flow cytometry
    Ekaterina I Galanzha
    Phillips Classic Laser and Nanomedicine Laboratories, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    Methods 57:280-96. 2012
    ..and plant vasculatures using photoacoustic (PA) detection techniques, multispectral high-pulse-repetition-rate lasers, tunable ultrasharp (up to 0...
  91. ncbi High-resolution photoreceptor imaging in idiopathic macular telangiectasia type 2 using adaptive optics scanning laser ophthalmoscopy
    Sotaro Ooto
    Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Kyoto, Japan
    Invest Ophthalmol Vis Sci 52:5541-50. 2011
    ..To study pathologic changes in the photoreceptors in eyes with idiopathic macular telangiectasia (MacTel) type 2 using adaptive optics scanning laser ophthalmoscopy (AO-SLO)...
  92. pmc In vivo ultra-fast photoacoustic flow cytometry of circulating human melanoma cells using near-infrared high-pulse rate lasers
    Dmitry A Nedosekin
    Philips Classic Laser and Nanomedicine Laboratories, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    Cytometry A 79:825-33. 2011
    ..Herewith, we also address sensitivity of label-free detection of melanoma CTCs and introduce in vivo CTC targeting by magnetic nanoparticles conjugated with specific antibody and magnetic cells enrichment...
  93. ncbi Direct laser writing of 3D scaffolds for neural tissue engineering applications
    V Melissinaki
    Foundation for Research and Technology Hellas FORTH, Institute of Electronic Structure and Laser IESL, N Plastira 100, 70013 Heraklion, Crete, Greece
    Biofabrication 3:045005. 2011
    ..Additionally, PC12 and NG108-15 neuroblastoma growth on bespoke scaffolds was studied in more detail to assess potential applications for neuronal implants of this material...
  94. ncbi Optofluidic integrated cell sorter fabricated by femtosecond lasers
    F Bragheri
    Istituto di Fotonica e Nanotecnologie IFN CNR, Dipartimento di Fisica Politecnico di Milano, P zza Leonardo da Vinci 32, 20133 Milano, Italy
    Lab Chip 12:3779-84. 2012
    ..This non-invasive device is specifically designed to operate with a limited amount of cells but with a very high selectivity in the sorting process. Characterization of the device with beads and validation with human cells are presented...
  95. ncbi Developing optofluidic technology through the fusion of microfluidics and optics
    Demetri Psaltis
    Department of Electrical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    Nature 442:381-6. 2006
    ..We categorize optofluidics according to three broad categories of interactions: fluid-solid interfaces, purely fluidic interfaces and colloidal suspensions. We describe examples of optofluidic devices in each category...
  96. ncbi Excitation of primary afferent neurons by near-infrared light in vitro
    Elizabeth J Katz
    Food and Drug Administration, Center for Devices and Radiological Health, Silver Spring, Maryland 20993, USA
    Neuroreport 21:662-6. 2010
    ..9 mV) suggests that LED is unrelated to the activation of heat-sensitive transient receptor potential cation channel subfamily V member 1 channels. The long duration of LEDs favors an involvement of second messengers...
  97. ncbi Laser-induced radiation microbeam technology and simultaneous real-time fluorescence imaging in live cells
    Stanley W Botchway
    Research Complex at Harwell, Central Laser Facility, STFC, Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxford, Oxfordshire, United Kingdom
    Methods Enzymol 504:3-28. 2012
    ....
  98. ncbi Effect of Er:YAG and Diode lasers on the adhesion of blood components and on the morphology of irradiated root surfaces
    Leticia Helena Theodoro
    Department of Periodontology, Araraquara Dental School, State University of Sao Paulo, Araraquara, Sao Paulo, Brazil
    J Periodontal Res 41:381-90. 2006
    ..94 microm) and GaAlAs Diode (808 nm) lasers and the effects on the morphology of irradiated root surfaces.
  99. ncbi Synthesis of bio-functionalized three-dimensional titania nanofibrous structures using femtosecond laser ablation
    Amirhossein Tavangar
    Department of Mechanical and Industrial Engineering, Ryerson University, 350 Victoria Street, Toronto, ON M5B 2K3, Canada
    Acta Biomater 7:2726-32. 2011
    ..This approach to synthesizing 3-D titania nanofibrous structures suggests considerable promise for the promotion of Ti interfacial properties to develop new functional biomaterials for various biomedical applications...
  100. ncbi Chromatic polarization effects of swept waveforms in FDML lasers and fiber spools
    Wolfgang Wieser
    Lehrstuhl fur BioMolekulare Optik, Fakultat fur Physik, Ludwig Maximilians Universitat Munchen, Oettingenstr 67, 80538 Munich, Germany
    Opt Express 20:9819-32. 2012
    We present detailed investigations of chromatic polarization effects, caused by fiber spools used in FDML lasers and buffering spools for rapidly wavelength swept lasers...
  101. ncbi ZnO nanowire lasers
    Daniel Vanmaekelbergh
    Debye Institute for NanoMaterials Science, University of Utrecht, PO Box 80 000, 3508 TA, Utrecht, The Netherlands
    Nanoscale 3:2783-800. 2011
    The pathway towards the realization of optical solid-state lasers was gradual and slow...

Research Grants87

  1. Short Wavelength UV Sources for Treatment of Psoriasis
    JOSEPH SMART; Fiscal Year: 2006
    ..are primarily broadband high pressure mercury lamps, mercury xenon, mercury argon and deuterium sources or excimer lasers. In practice, this has meant that phototherapy treatment methods have had to be adapted to the available light ..
  2. Portable Retinal Imaging Device for Urgent Referrals and Remote Treatment
    Matthew S Muller; Fiscal Year: 2012
    ..and edema with a portable device, Aeon aims to encourage retinal specialists to deliver treatment with portable lasers or intravitrea injections in the community/multi-specialty clinic setting...
  3. Real-Time Reagent-Free Noninvasive Diagnosis of Tuberculosis
    Payam Nahid; Fiscal Year: 2013
    ..SPAMS functions by drawing in individual aerosol particles and tracking them with lasers as they proceed towards the center of the source region of a dual-polarity time-of-flight mass spectrometer...
  4. Low Cost Multi-pathogen Laser Diagnostic for HIV and AIDS Co-Infections
    CHRISTOPHER JOHN MYATT; Fiscal Year: 2011
    ..g., DVD player lasers) and optics (consumer and cell phone cameras)...
  5. Automated Microscope for Long-Duration, Quantitative Dynamic Imaging
    JOHN PETER WIKSWO; Fiscal Year: 2010
    ..spinning disk confocal microscope with a 10x air objective and 20x and 60x automated water objectives, three lasers and a tunable UV source, IR autofocus, two cameras that enable simultaneous two-color imaging and sequential ..
  6. The Molecular Structure of the RNA Polymerase Portal of a Bacteriophage-Reovirus
    PAUL JEFFREY GOTTLIEB; Fiscal Year: 2013
    ..This research is performed in collaboration with physicists at the Institute for Ultrafast Spectroscopy and Lasers (IUSL) on the CCNY campus...
  7. IR-LAMP: Optigenetic technology to spatially manipulate protein function in vivo
    Julie C Canman; Fiscal Year: 2011
    ..Further, as infrared lasers can penetrate deep into tissues, IRLAMP could also be adopted for use in humans to treat localized diseases, such ..
  8. FORCE SPECTROSCOPY AND STRUCTURAL BIOLOGY OF HAIR-CELL TIP LINKS
    Marcos Sotomayor; Fiscal Year: 2012
    ..This involves learning how to use and align lasers, how to design and synthesize linkers between molecules and functionalized, micrometer-size polystyrene beads, as ..
  9. Diffraction Methods in Molecular Biology Gordon Research Conference
    NANCY RYAN GRAY; Fiscal Year: 2010
    ..oral presentation of selected posters, model building and imaging methods of the future such as free electron lasers and diffractive imaging...
  10. Breath Analysis using Quantum Cascade Lasers
    JOANNE SHORTER; Fiscal Year: 2009
    ..It will also be a powerful research tool for investigating exposures that initiate and exacerbate asthma and COPD. ..
  11. Leica TCS SP5 MP confocal and two-photon microscope
    Martin D Brand; Fiscal Year: 2012
    ..Additionally, a 442 nm and other standard visible lasers, in combination with three internal detector spectral imaging will facilitate versatle single photon, confocal ..
  12. Optical probes for controlling cellular function
    GRAHAM ELLIS-DAVIES; Fiscal Year: 2013
    ..v.-absorbing chromophores. Uncaging using such lasers is produced by the simultaneous absorption of two red photons of equivalent energy to one blue photon...
  13. Laser System for Clinical Photoacoustic Imaging Applications
    DONALD F HELLER; Fiscal Year: 2013
    ..penetration and tumor localization, the key wavelengths of interest lie within the tuning range of alexandrite lasers (or of alexandrite and Nd:YAG lasers (700 - 1100 nm)...
  14. ZEISS LSM710 SCANNING CONFOCAL MICROSCOPE
    Hazel L Sive; Fiscal Year: 2010
    ..offered by the LSM710 include a 405nm wavelength laser, which, in conjunction with the LSM710's five other lasers, allows simultaneous multi-imaging of up to ten different dyes;a new spectral recycling loop and a twin gate beam ..
  15. A novel anti-scar peptide for cutaneous wound repair
    Chia Soo; Fiscal Year: 2013
    ..g., silicone sheeting, topical ointments, and lasers)...
  16. Near-infrared laser to replace chemical vaccine adjuvant
    Satoshi Kashiwagi; Fiscal Year: 2013
    ..However, these lasers require co-administration of an additional chemical adjuvant to induce a sufficiently increased immunological ..
  17. Retinal Laser Burn Abolishes Ocular Immunity Privilege
    Kenyatta G Lucas; Fiscal Year: 2010
    ..Recently, we have found that retinal laser burn (RLB) abolishes ACAID. This is of interest because lasers are gaining increase usage for treatment of glaucoma, myopia and Age-related macular degeneration...
  18. FORCE SPECTROSCOPY AND STRUCTURAL BIOLOGY OF HAIR-CELL TIP LINKS
    Marcos Sotomayor; Fiscal Year: 2013
    ..This involves learning how to use and align lasers, how to design and synthesize linkers between molecules and functionalized, micrometer-size polystyrene beads, as ..
  19. Surgical Laser device development for precise or bulk ablation of urologic tissue
    Jonathon D Wells; Fiscal Year: 2010
    ..Surgical lasers form the standard of care for many treatments and endoscopic procedures in the prostate, kidney, ureter, bladder, ..
  20. Laser Device Development and Qualification to Enable Laser Tissue Welding
    CRAIG LENNON; Fiscal Year: 2010
    VISOTEK, Inc. is an OEM focusing its R&D and product development on novel diode lasers, systems and components that simplify the use of lasers in commercial, military, medical and research environments...
  21. Quantitative Measurement of Isotope Ratios by TOF-SIMS MS
    Marvin L Vestal; Fiscal Year: 2012
    ..ion source that provides several orders of magnitude greater ionization rates that are possible with the pulsed lasers used in earlier work...
  22. Laser-Enhanced NMR Instrumentation for Chemical and Biochemical Research
    Silvia Cavagnero; Fiscal Year: 2013
    ..the acquisition of a new console for our 600 MHz NMR spectrometer, a cryogenically-cooled probe, three high-power lasers (25 W frequency-tunable Argon ion, excimer, and Ti-Sapphire) for photo-CIDNP-mediated ultrasensitive NMR and laser-..
  23. Olympus TIRF Microscope for Simultaneous Multichannel Live cell Imaging
    RADU VIRGIL STAN; Fiscal Year: 2013
    ..multicolor total internal reflection microscopy (TIRFM) illuminator (Olympus Celltirf) equipped with three lasers 405/491/561, and capable of FRAP/ photoactivation;an ultra-fast/highly sensitive sCMOS camera (Andor Neo) coupled ..
  24. Improved stent design through hemodynamics and vascular biology
    Juan Miguel Jimenez; Fiscal Year: 2013
    ..Aim 1 will test the hypothesis that flow in the vicinity of stent struts is highly influenced by strut geometry. Lasers and Partice Image Velocimetry (PIV) will be used to quantify flow disturbances in coronary artery hemodynamic ..
  25. Upgrade of current CompuCyte LSC??? to the CompuCyte iCys??? Laser Scanning Cytom
    Kevan Roberts; Fiscal Year: 2010
    ..The upgrade takes advantage of using some of the existing parts of the LSC, including the lasers and video camera with monitor, significantly lowering the cost of purchasing a new system...
  26. Olympus BX-DSU Live-Cell Disk Scanning Imaging / Electrophysiology System
    Bradley K Taylor; Fiscal Year: 2010
    ..The broad spectrum light source produces substantially less photobleaching than do lasers. Furthermore, this system adds a UV flash illuminator for photolysis of photoactivatable (caged) compounds, ..
  27. Acquisition of a Carl Zeiss Cell Observer Spinning Disk Confocal Microscope
    J Michael McCaffery; Fiscal Year: 2013
    ..inverted research microscope, integrates a Yokogawa spinning disk confocal with: 1) 405nm/488nm/561nm/635nm lasers;2) two ultra-sensitive Evolve EMCCD cameras for simultaneous two-channel imaging;3) piezo-electric focusing for ..
  28. Controlling neural circuits with single-cell resolution in behaving animals
    Christopher Fang-Yen; Fiscal Year: 2013
    ..elegans. This project extends previous work by Dr. Fang-Yen, in which machine vision algorithms and lasers patterned by a digital micromirror device (DMD) were used to achieve spatiotemporal control of neural activity in ..
  29. Compact Hydrogen Peroxide Sensor for Sterilization Cycle Monitoring
    Krishnan Parameswaran; Fiscal Year: 2013
    ..measure VPHP and water vapor using tunable diode laser absorption spectroscopy (TDLAS) with novel quantum cascade lasers and cavity enhanced spectroscopy...
  30. Can light sensors, placed in ganglion cells, restore vision to a blind retina?
    William H Merigan; Fiscal Year: 2010
    ..causes blindness in many common eye diseases, such as macular degeneration, as well as in blinding exposure to lasers or other intense lights...
  31. Untethering Electrophysiology in Vivo: An Optical Nanoscale Semiconductor Array f
    AUREL A LAZAR; Fiscal Year: 2013
    ..Our proposal calls for the development of nanoscale neural probes based on photonic crystal (PC) nanocavity lasers that can be used for untethered neural sensing and recording in-vivo...
  32. Recording neural activities onto DNA
    Konrad P Kording; Fiscal Year: 2013
    ..of recorded neurons is relatively small because current neural recording devices, such as electrodes, amplifiers, lasers, and cameras, are macroscopic...
  33. Molecular Fluorescent Toolkit
    VLADISLAV VERKHUSHA; Fiscal Year: 2013
    ..With the improvement of two-photon lasers, a deep-tissue intravital imaging in live animals has become widely available...
  34. Confocal upgrade for intravital microscopy following vascular injury
    Lawrence F Brass; Fiscal Year: 2010
    ..The requested funds will be used to upgrade the confocal head, lasers and camera, and replace the wavelength changer with one that is much faster...
  35. Portable Instrument For Assessing Zinc Deficiency In Children and The Elderly
    Christopher J Frederickson; Fiscal Year: 2013
    ..Modern solid-state lasers, CCDs, photodiodes, and ICs have allowed the entire instrument to be shrunk to the size and form-factor shown for ..
  36. BD Influx 5 Laser Fluorescence Activated Cell Sorter
    David A Thorley-Lawson; Fiscal Year: 2013
    ..We are now asking for an Influx cell sorter with 5 lasers (BD Biosciences)...
  37. Therapeutic Window for Acute Stroke Therapy (P50)
    Brett C Meyer; Fiscal Year: 2012
    ..stroke patients within 0-48 hours of symptom onset;(5) Safety of tPA plus Transcranial Emission of Low-Energy Lasers for Acute Stroke Recovery (StELLAR), the first combination study of the NeuroThera Laser System with rt-PA;and (6) ..
  38. Fluorescence Lifetime Imaging Microscopy (FLIM)
    Joseph R Lakowicz; Fiscal Year: 2010
    ..wavelengths provided by laser diode sources are suitable for various experiments and we are able to use the other lasers available in the CFS using the access port of the instrument, including our Ti:Sapphire system for two-photon FLIM ..
  39. Spatial Analysis of Molecules in Tissue using MALDI-MS
    Richard M Caprioli; Fiscal Year: 2013
    ..10 um) will be employed with tissue preparation protocols that provide 1 um matrix crystal sizes and high speed lasers (.3 kHz) to provide data on multiple serial sections in a efficacious and timely manner...
  40. Clonal Analysis of Neocortical Interneuron Circuit Development
    Song Hai Shi; Fiscal Year: 2011
    ..g. two photon lasers scanning microscopy) and electrophysiology (e.g...
  41. Miniaturized Laser Sources for Optical Neuroprostheses
    MATTHEW DUMMER; Fiscal Year: 2012
    ..Vertical Cavity Surface Emitting Lasers (VCSELs) hold great promise for providing the combination of size and performance that are required...
  42. A LASER MICROBEAM BIOTECHNOLOGY RESOURCE
    Bruce J Tromberg; Fiscal Year: 2011
    ..Laser Microbeam and Medical Program (LAMMP) is a NIH Biomedical Technology Resource Center dedicated to the use of lasers and optics in biology and medicine...
  43. Structure and Biomechanics of the Human Optic Nerve Head
    Donald J Brown; Fiscal Year: 2013
    ..take advantage of known non-linear optical affects that occur when high intensity photons generated by ultrafast lasers interact with tissue...
  44. Leica LMD7000 Laser Microdissection System
    Viktor Kharazia; Fiscal Year: 2010
    ..Recently, LCM vendors have developed systems that use ultraviolet (UV) lasers as a cutting tool and this development has proven to be a major improvement...
  45. Methamphetamine and HIV-1 Infection: Targeted Nanoparticles for Gene Silencing.
    Jessica L Reynolds; Fiscal Year: 2012
    ..She also will utilize the facilities of the Institute for Lasers, Photonics and Biophotonics on the main campus of the University at Buffalo...
  46. Multi-wavelength femtosecond laser sources for intravital multiphoton microscopy
    CHRIS XU; Fiscal Year: 2013
    ..3) A single light source will enable experiments that currently require two synchronized Ti:sapphire lasers plus an optical parametric oscillator (OPO) and a regenerative amplifier...
  47. BioCARS: A Synchroton Structural Biology Resource
    JOHN KEITH MOFFAT; Fiscal Year: 2011
    ..A laser laboratory houses ps and ns pulsed lasers and other optical instrumentation essential to the time-resolved experiments...
  48. Temperature monitoring in moving organs during thermal ablation
    Lawrence P Panych; Fiscal Year: 2013
    ..Heat may be delivered with lasers, RF antennas, or ultrasound transducers, and magnetic resonance (MR) thermometry has been used to monitor thermal ..
  49. 2012 Diffraction Methods in Molecular Biology Gordon Research Conference
    Ana Gonzalez; Fiscal Year: 2012
    ..an emphasis on membrane proteins), structure validation and imaging and diffraction experiments at free electron lasers. PUBLIC HEALTH RELEVANCE: The 2012 Gordon Research Conference in Diffraction Methods in Structural Biology seeks ..
  50. Acquisition of a DeltaVision OMX Super-Resolution Imaging System
    Claire E Walczak; Fiscal Year: 2010
    ..The power of this system is that the super resolution is achieved with conventional lasers and computer technology, the microscope system is a standard high-end widefield microscope, conventional ..
  51. Confocal enhanced episcopic fluorescent image capture (EFIC)
    Cecilia W Lo; Fiscal Year: 2012
    ..In addition, with appropriate lasers and excitation/emission filters, EFIC imaging can be combined with the tracking of specific cell lineages using ..
  52. Development of a Novel Laser Instrument for Advanced Medical Applications
    Claus Peter Richter; Fiscal Year: 2011
    ..Currently, the application of medical lasers is starkly limited by the lack of flexibility and versatility of most commercial laser systems...
  53. Patterned Microtexture for Improved Durability of Orthopedic Knee Implants
    RONALD L JACOBSEN; Fiscal Year: 2013
    ..Newly available ultra short pulse lasers will b used to create the designed texture on a CoCr femoral component in the form of arrays of micron-scale ..
  54. BD FACSAria System
    Edward F Srour; Fiscal Year: 2013
    ..therefore requesting funds to acquire a Special Becton Dickinson FACSAria System for cell sorting composed of five lasers and contained completely within a BSL2/BSL3 biosafety cabinet...
  55. Laser-based spectrometer for real-time monitoring of VOCs at Superfund sites
    JOHN BRIAN LEEN; Fiscal Year: 2012
    ..cavity ringdown spectroscopy (iCRDS) technology, which utilizes a broadly tunable external cavity quantum cascade lasers (8.3 - 12...
  56. BioCARS: A Synchroton Structural Biology Resource
    JOHN KEITH MOFFAT; Fiscal Year: 2012
    ..A laser laboratory houses ps and ns pulsed lasers and other optical instrumentation essential to the time-resolved experiments...
  57. Novel caged Dopamine compounds
    RAFAEL M YUSTE; Fiscal Year: 2013
    ..We propose to use a newly synthesized caged compound, RuBi-Dopa, which can be photoreleased with two-photon lasers, to optically activate dopamine receptors with high precision and map the distribution of functional dopamine ..
  58. BD FACS ARIA II CELL SORTER
    Qizhi Tang; Fiscal Year: 2010
    ..We have custom configured the FACSAria II to have four lasers at 405 nm, 488 nm, 561 nm, and 640 nm and twelve photomultiplier tubes for simultaneous analysis of ten ..
  59. Low Light Level Camera For Raman Spectroscopy
    Jie Yao; Fiscal Year: 2011
    ..extending beyond 1000 nm, where the tissue fluorescence is completely suppressed and narrow line width diode lasers, such as those DFB lasers developed for optical communication applications, are readily available...
  60. 2010 GRC on Lasers in Medicine and Biology; Gordon Research Conference
    Lihong Wang; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): The 2010 Gordon Research Conference on Lasers in Medicine and Biology will be held from July 25-30, 2010 at the Holderness School, Holderness, New Hampshire...
  61. Mid-IR LIDAR for Detecting Volatile Organic Compounds
    IGOR E TROFIMOV; Fiscal Year: 2010
    ..analysis will be enabled thorough application of novel mid-IR widely tunable external cavity quantum cascade lasers with mode-hop-free high resolution wavelength tuning capability...
  62. Advanced Laser Technology for Fractional Skin Resurfacing and Other Surgical Appl
    RAVINDER JAIN; Fiscal Year: 2010
    ..In the near-term, these instruments will act as efficient replacements for Er:YAG and Er:YSGG lasers used in several dermatological applications...
  63. Port wine stain treatment for infants and young children
    Guillermo Aguilar; Fiscal Year: 2006
    ..The proposed studies investigate the appropriate use of yellow light emitted by pulsed dye lasers in combination with short cryogen spurts to improve the therapeutic outcome of port wine stains (PWS) in infants ..
  64. FACSVantage SE Upgrade
    RAYMOND HESTER; Fiscal Year: 2004
    ..FACSVantage SE flow cytometer to include DiVa electronics, TurboSort, and the replacement of fifteen-year old lasers. The projects included in this application are being conducted with faculty in five departments within the ..
  65. LuAG:Ce,Zr Scintillators for PET
    KEITH ROZENBURG; Fiscal Year: 2007
    ..Learning from the development of transparent yttrium aluminum garnet (YAG) for lasers and TA&T's own developments of transparent magnesium aluminate among other transparent ceramics we will ..
  66. Oxide Glass Fiber Optimized for Short Pulse IR Lasers
    Danh Tran; Fiscal Year: 2006
    ..79 mu/m) and Er:YAG (2.94 mu/m) lasers for therapeutic procedures...
  67. Laser Ablation of Dental Hard Tissues
    Daniel Fried; Fiscal Year: 2006
    ..In addition to eliminating the noise and vibration associated with the Dental drill, lasers offer several unique advantages over current surgical technology for conservative Dentistry...
  68. 2007 Electron Distribution & Chemical Bonding Gordon Research Conference
    Carlo Gatti; Fiscal Year: 2007
    ..TR) spectroscopy and crystallographic studies and, especially, in 5-10 years, of the X-ray Free Electron Lasers (XFEL) international facilities, the scenario of structural biology is going to amazingly change...
  69. Real-time Single-Molecule Analysis for Transient Molec. Complex Characterization
    Taiho Kim; Fiscal Year: 2009
    ..In two-color (2c)-ALEX, employing two alternating lasers to study molecular interactions (through probe stoichiometry ratio S and/or FRET efficiency E) and intramolecular ..
  70. CYTOMATION MOFLO MLS FLOW CYTOMETER
    Michael Axthelm; Fiscal Year: 1999
    This application requests funds to purchase a Cytomation MoFlo MLS (MoFlo). The MoFlo is equipped with three lasers, two scatter and six fluorescence detectors, and can sort 2, 3 or 4 ways at a rate of 25,000 cells/second and clone cells ..
  71. SHARED FLOW CYTOMETRY FACILITY (FACSTAR PLUS)
    ROSS BASCH; Fiscal Year: 1991
    ..to perform 3 color analysis with the argon laser and 4 color analysis with the combination of argon and dye lasers. Three fluorescence signals can be analyzed (and sorted) with a single laser...
  72. 3RD CONGRESS INTL SOCIETY FOR LASERS IN DENTISTRY
    G Powell; Fiscal Year: 1992
    The 3rd Congress of the International Society for Lasers in Dentistry will be hosted by the Division of Dental Education and the Laser Institute of the Health Science Center at the University of Utah on August 6-8, 1992 in Salt Lake City, ..
  73. LASER PHOTOTHERAPY FOR THE TREATMENT OF MALIGNANCIES
    Dan Castro; Fiscal Year: 1992
    Photodynamic therapy with lasers may become an attractive adjunctive modality for early diagnosis and treatment of superficial malignancies when fluorochromes with high tumor specificity and low toxicity are developed...
  74. LASER EFFECTS ON DENTAL HARD TISSUES
    John Featherstone; Fiscal Year: 2007
    ..with dental hard tissues (enamel and dentin) and to establish a scientific basis for the safe and effective use of lasers on these tissues...
  75. Advanced Intravital Microscope
    Urs Utzinger; Fiscal Year: 2007
    ..features available in advanced microscopy systems have resulted from the development of rapidly tunable excitation lasers, sophisticated multi-channel detection systems and advanced photon counting electronics allowing time correlated ..
  76. OPTIMAL LASER FOR SMALL DIAMETER VASCULAR ANASTOMOSES
    Robert Kung; Fiscal Year: 1990
    ..The proposed laser has several advantages over the more commonly used C0-2 and Ar lasers. Unlike the C0-2 laser, 1...
  77. ULTRAFAST MULTIPHOTON LASER ACTIVATION SYSTEM
    WATT WEBB; Fiscal Year: 2001
    ..Ultrafast (approximately 100 femtosecond) pulses generated by mode-locked lasers allow simultaneous absorption of two or more photons from bright pulsed illumination for molecular excitation as ..
  78. IR LASER ABLATION OF DENTAL HARD TISSUE
    Daniel Fried; Fiscal Year: 2001
    ..a basic mechanistic approach to understanding ablation of dental hard tissue during irradiation with infrared (IR) lasers. The overall objective of these studies is to determine the specific laser irradiation conditions, namely ..
  79. Acquisition of FACSVantage SE Flow Cytometer
    Yair Gazitt; Fiscal Year: 2004
    ..The base of the lab is a 20 year old FACSstar Plus and a 2 year old FACSCalibur analyzer equipped with 2 lasers. The FACStarPlus is equipped with a 70-CR599-dye laser with far-red excitation and an Argon laser with a standard ..
  80. COMPACT LASER INSTRUMENTS FOR OTOLOGIC SURGERY
    JEFFREY PIERCE; Fiscal Year: 2007
    ..7 micron ErZBLAN fiber lasers. The Phase I program has demonstrated feasibility for the 2...
  81. LASER SCANNING CONFOCAL MICROSCOPE
    Robert Lechleider; Fiscal Year: 2003
    ..The three lasers requested, argon, red Helium-Neon and green Helium-Neon, will provide excitatory signals for most applications, ..
  82. ZEISS LSM 510 CONFOCAL MICROSCOPE SYSTEM
    Richard Kurten; Fiscal Year: 2005
    ..We believe that the Zeiss LSM 510 with multiple lasers (405 nm to 633 nm) offers the best-integrated system available...
  83. Mid-Career Investigator in Patient-Oriented Research
    John Nelson; Fiscal Year: 2007
    ..D., Ph.D. training, academic affiliation, and involvement in the basic science and clinical applications of lasers make him uniquely qualified and experienced to pursue a career in patient oriented research...
  84. MICROWAVE PLAQUE WELDING FOLLOWING BALLOON ANGIOPLASTY
    CAREY RAPPAPORT; Fiscal Year: 1990
    ..This has previously been accomplished primarily through the user of laser energy. As a possible alternative to lasers, this microwave design will be an easier to use, safer, and less complex method of welding atheromatous plaque ..
  85. Upgrade of Femtosecond Laser Sources for Optical Workstation
    KEVIN ELICEIRI; Fiscal Year: 2007
    ..Two femtosecond laser sources are requested. Both sources will replace/upgrade existing lasers: an older Ti:Sapphire laser and a now defunct 1047 laser...
  86. Novel Cancer Nanotechnology Platforms for Photodynamic Therapy and Imaging
    Ravindra Pandey; Fiscal Year: 2009
    ..development partnership is led by Roswell Park Cancer Institute, with the collaboration of the Institute for Lasers, Photonics and Biophotonics at the University of Buffalo, and the Department of Chemistry at the University of ..
  87. AN OPTIMAL LASER FOR SMALL DIAMETER VASCULAR ANASTOMOSES
    Robert Kung; Fiscal Year: 1993
    ..The proposed laser has several advantages over the more commonly used CO2 and Ar lasers. Unlike the CO2 laser, the 1...