serum free culture media

Summary

Summary: CULTURE MEDIA free of serum proteins but including the minimal essential substances required for cell growth. This type of medium avoids the presence of extraneous substances that may affect cell proliferation or unwanted activation of cells.

Top Publications

  1. ncbi Functional characterization of monocyte-derived dendritic cells generated under serumfree culture conditions
    Manfred Lehner
    Department of Cellular Therapy, Children s University Hospital, Erlangen, Germany
    Immunol Lett 99:209-16. 2005
  2. ncbi The small GTP-binding protein rac regulates growth factor-induced membrane ruffling
    A J Ridley
    Institute for Cancer Research, Chester Beatty Laboratories, London, England
    Cell 70:401-10. 1992
  3. ncbi Forced aggregation of defined numbers of human embryonic stem cells into embryoid bodies fosters robust, reproducible hematopoietic differentiation
    Elizabeth S Ng
    Monash Immunology and Stem Cell Laboratories, Monash University, Bldg 75, STRIP 1, West Ring Rd, Clayton, Victoria, 3800 Australia
    Blood 106:1601-3. 2005
  4. ncbi Neuroprotection by brain-derived neurotrophic factor is mediated by extracellular signal-regulated kinase and phosphatidylinositol 3-kinase
    M Hetman
    Toxicology Program, Department of Environmental Health, the Graduate Program in Neurobiology and Behavior, The Graduate Program in Molecular and Cell Biology, University of Washington, Seattle, Washington 98195 7234, USA
    J Biol Chem 274:22569-80. 1999
  5. ncbi Optimization of microcarrier cell culture process for the inactivated enterovirus type 71 vaccine development
    Suh Chin Wu
    Department of Life Science, Institute of Biotechnology, National Tsing Hua University, Hsinchu, Taiwan
    Vaccine 22:3858-64. 2004
  6. ncbi Directed differentiation of telencephalic precursors from embryonic stem cells
    Kiichi Watanabe
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe 650 0047, Japan
    Nat Neurosci 8:288-96. 2005
  7. pmc Persistence of a small subpopulation of cancer stem-like cells in the C6 glioma cell line
    Toru Kondo
    Department of Cell Fate Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, 2 2 1 Honjo, Kumamoto 860 0811, Japan
    Proc Natl Acad Sci U S A 101:781-6. 2004
  8. ncbi Stromal cells prevent apoptosis of AML cells by up-regulation of anti-apoptotic proteins
    M Konopleva
    Section of Molecular Hematology and Therapy, Department of Blood and Marrow Transplantation, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Leukemia 16:1713-24. 2002
  9. ncbi Defined conditions for neural commitment and differentiation
    Qi Long Ying
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh, Scotland, EH9 3JQ, UK
    Methods Enzymol 365:327-41. 2003
  10. pmc Rac mediates cytoskeletal rearrangements and increased cell motility induced by urokinase-type plasminogen activator receptor binding to vitronectin
    L Kjøller
    Medical Research Council Laboratory for Molecular Cell Biology, Cancer Research Campaign Oncogene and Signal Transduction Group, University College London, United Kingdom
    J Cell Biol 152:1145-57. 2001

Detail Information

Publications208 found, 100 shown here

  1. ncbi Functional characterization of monocyte-derived dendritic cells generated under serumfree culture conditions
    Manfred Lehner
    Department of Cellular Therapy, Children s University Hospital, Erlangen, Germany
    Immunol Lett 99:209-16. 2005
    ....
  2. ncbi The small GTP-binding protein rac regulates growth factor-induced membrane ruffling
    A J Ridley
    Institute for Cancer Research, Chester Beatty Laboratories, London, England
    Cell 70:401-10. 1992
    ..We propose that rac and rho are essential components of signal transduction pathways linking growth factors to the organization of polymerized actin...
  3. ncbi Forced aggregation of defined numbers of human embryonic stem cells into embryoid bodies fosters robust, reproducible hematopoietic differentiation
    Elizabeth S Ng
    Monash Immunology and Stem Cell Laboratories, Monash University, Bldg 75, STRIP 1, West Ring Rd, Clayton, Victoria, 3800 Australia
    Blood 106:1601-3. 2005
    ..This method of EB formation provides a generally applicable means for modulating and objectively monitoring the directed differentiation of hESCs...
  4. ncbi Neuroprotection by brain-derived neurotrophic factor is mediated by extracellular signal-regulated kinase and phosphatidylinositol 3-kinase
    M Hetman
    Toxicology Program, Department of Environmental Health, the Graduate Program in Neurobiology and Behavior, The Graduate Program in Molecular and Cell Biology, University of Washington, Seattle, Washington 98195 7234, USA
    J Biol Chem 274:22569-80. 1999
    ..Furthermore, the relative contribution of the ERK and PI 3-kinase pathways to neuronal survival may depend on the type of cellular injury...
  5. ncbi Optimization of microcarrier cell culture process for the inactivated enterovirus type 71 vaccine development
    Suh Chin Wu
    Department of Life Science, Institute of Biotechnology, National Tsing Hua University, Hsinchu, Taiwan
    Vaccine 22:3858-64. 2004
    ..These results constitute valuable information on the development of a large-scale microcarrier cell culture process for producing inactivated EV71 vaccine...
  6. ncbi Directed differentiation of telencephalic precursors from embryonic stem cells
    Kiichi Watanabe
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe 650 0047, Japan
    Nat Neurosci 8:288-96. 2005
    ..Thus, in the absence of caudalizing signals, floating aggregates of ES cells generate naive telencephalic precursors that acquire subregional identities by responding to extracellular patterning signals...
  7. pmc Persistence of a small subpopulation of cancer stem-like cells in the C6 glioma cell line
    Toru Kondo
    Department of Cell Fate Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, 2 2 1 Honjo, Kumamoto 860 0811, Japan
    Proc Natl Acad Sci U S A 101:781-6. 2004
    ..We propose that many cancer cell lines contain a minor subpopulation of stem cells that is enriched in an SP, can be maintained indefinitely in culture, and is crucial for their malignancy...
  8. ncbi Stromal cells prevent apoptosis of AML cells by up-regulation of anti-apoptotic proteins
    M Konopleva
    Section of Molecular Hematology and Therapy, Department of Blood and Marrow Transplantation, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Leukemia 16:1713-24. 2002
    ..The observed association of high Bcl-2 expression in stroma-supported AML blasts in vitro with resistance to chemotherapy in vivo suggests that the same mechanisms may be operational in vivo...
  9. ncbi Defined conditions for neural commitment and differentiation
    Qi Long Ying
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh, Scotland, EH9 3JQ, UK
    Methods Enzymol 365:327-41. 2003
    ....
  10. pmc Rac mediates cytoskeletal rearrangements and increased cell motility induced by urokinase-type plasminogen activator receptor binding to vitronectin
    L Kjøller
    Medical Research Council Laboratory for Molecular Cell Biology, Cancer Research Campaign Oncogene and Signal Transduction Group, University College London, United Kingdom
    J Cell Biol 152:1145-57. 2001
    ..This mechanism may play a role in uPAR-mediated regulation of cell motility at sites where VN and uPAR are co-expressed, such as malignant tumors...
  11. ncbi Development rates of male bovine nuclear transfer embryos derived from adult and fetal cells
    J R Hill
    Department of Veterinary Physiology and Pharmacology, Department of Large Animal Medicine and Surgery, Texas A and M University, College Station, Texas 77843, USA
    Biol Reprod 62:1135-40. 2000
    ..Serum starvation of these adult donor cells did not improve development rates of NT embryos to blastocyst, but when fetal cells were serum-starved, there was a significant increase in development to blastocyst...
  12. ncbi Direct neural fate specification from embryonic stem cells: a primitive mammalian neural stem cell stage acquired through a default mechanism
    V Tropepe
    Department of Anatomy and Cell Biology, University of Toronto, Ontario M5S 1A8, Toronto, Canada
    Neuron 30:65-78. 2001
    ..These results are consistent with a default mechanism for neural fate specification and support a model whereby definitive neural stem cell formation is preceded by a primitive neural stem cell stage during neural lineage commitment...
  13. pmc Targeted disruption of the three Rb-related genes leads to loss of G(1) control and immortalization
    J Sage
    Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 14:3037-50. 2000
    ....
  14. ncbi Stationary and microcarrier cell culture processes for propagating Japanese encephalitis virus
    Suh Chin Wu
    Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan 30013
    Biotechnol Prog 18:124-8. 2002
    ..Thus, the stationary culture is the most efficient system for JEV production from cultured Vero cells...
  15. ncbi Serum-free spheroid culture of mouse corneal keratocytes
    Satoru Yoshida
    Cornea Center and Departmernt of Ophthalmology, Tokyo Dental College, Chiba, Japan
    Invest Ophthalmol Vis Sci 46:1653-8. 2005
    ..To develop a serum-free mass culture system for mouse keratocytes...
  16. ncbi Culture medium and protein supplementation in the generation and maturation of dendritic cells
    P J Royer
    INSERM U601, Institut de Biologie, Nantes, France
    Scand J Immunol 63:401-9. 2006
    ..This underscores the importance of defining culture medium composition in clinical standard operating procedures to insure quality control, and also when preparing DC for experimental uses...
  17. ncbi Morphological and functional changes of rabbit mesenteric artery cultured with fetal bovine serum
    H Yamawaki
    Department of Veterinary Pharmacology, Radio Isotope Center, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Japan
    Life Sci 67:807-20. 2000
    ..NO production in smooth muscle cells is also responsible for the decreased contractility after the serum-treatment...
  18. pmc Human cytomegalovirus prevents replication licensing by inhibiting MCM loading onto chromatin
    Lüder Wiebusch
    Department of Pediatrics, Laboratory for Molecular Biology, Charite, CCM Ziegelstrasse, 5 9, Humboldt University, 10098 Berlin, Germany
    EMBO Rep 4:42-6. 2003
    ..Thus, this particularly large virus might thereby secure the supply with essential replication factors but omit competitive cellular DNA replication...
  19. pmc VIP and PACAP are autocrine factors that protect the androgen-independent prostate cancer cell line PC-3 from apoptosis induced by serum withdrawal
    Irene Gutierrez-Cañas
    Department of Biochemistry and Molecular Biology, University of Alcala, E 28871 Alcala de Henares, Spain
    Br J Pharmacol 139:1050-8. 2003
    ..5. Our results suggest that VIP and PACAP are autocrine/paracrine factors that protect PC-3 cells from apoptosis through VPAC1 receptors...
  20. ncbi Animal cell cultures: recent achievements and perspectives in the production of biopharmaceuticals
    Michael Butler
    Department of Microbiology, University of Manitoba, Buller Building, Winnipeg, Canada
    Appl Microbiol Biotechnol 68:283-91. 2005
    ..However, some analysts indicate that this still may not be enough and that future manufacturing demand may exceed production capacity as the number of approved biotherapeutics increases...
  21. ncbi Ghrelin modulates the downstream molecules of insulin signaling in hepatoma cells
    Masahiro Murata
    Third Division and Second Division, Department of Medicine and the Department of Basic Allied Medicine, Kobe University School of Medicine, Kobe, Japan
    J Biol Chem 277:5667-74. 2002
    ..Unlike insulin, ghrelin inhibited Akt kinase activity as well as up-regulated gluconeogenesis. These findings raise the possibility that ghrelin modulates insulin activities in humans...
  22. ncbi ERK1/2 antagonizes glycogen synthase kinase-3beta-induced apoptosis in cortical neurons
    Michal Hetman
    Departments of Environmental Health and Pharmacology, University of Washington, Seattle, WA 98195 7234, USA
    J Biol Chem 277:49577-84. 2002
    ..Furthermore, ERK1/2 inhibits GSK3beta activity via a novel mechanism that is independent of Ser-9 phosphorylation and likely does not involve Tyr-216 phosphorylation...
  23. ncbi Development of a long-term bovine granulosa cell culture system: induction and maintenance of estradiol production, response to follicle-stimulating hormone, and morphological characteristics
    C G Gutierrez
    Roslin Institute, Roslin, Midlothian, United Kingdom
    Biol Reprod 56:608-16. 1997
    ..This system will permit the detailed study of the key factors controlling the differentiation and proliferation of bovine granulosa cells...
  24. ncbi Detection, isolation, and stimulation of quiescent primitive leukemic progenitor cells from patients with acute myeloid leukemia (AML)
    Yinghui Guan
    Terry Fox Laboratory, British Columbia Cancer Agency, and the Department of Medicine, University of British Columbia, Vancouver, Canada
    Blood 101:3142-9. 2003
    ..In summary, quiescent leukemic cells, including NOD/SL-ICs, are present in most AML patients. Their spontaneous entry into active cell cycle in short-term culture might be explained by the deregulated GF signaling present in many AMLs...
  25. ncbi Enhanced survivability of cloned calves derived from roscovitine-treated adult somatic cells
    John Gibbons
    Department of Animal and Dairy Sciences, University of Georgia, Athens, GA 30602, USA
    Biol Reprod 66:895-900. 2002
    ..Roscovitine culture can predictably synchronize the donor cell cycle and increase the nuclear reprogramming capacity of the cells, resulting in enhanced fetal and calf survival and increased cloning efficiency...
  26. ncbi A comparative analysis of serum and serum-free media for generation of clinical grade DCs
    Chiara Napoletano
    Department of Experimental Medicine, University of Rome La Sapienza, Viale Regina Elena 324, 00161 Rome, Italy
    J Immunother 30:567-76. 2007
    ..In conclusion, a comparative analysis of DC performance generated in different conditions allows us to determine CellGro DC as the optimal medium for the generation of clinical grade DCs...
  27. ncbi Serum-free hybridoma culture: ethical, scientific and safety considerations
    Megha S Even
    Physicians Committee for Responsible Medicine, 5100 Wisconsin Ave NW, Suite 400, Washington, DC 20016, USA
    Trends Biotechnol 24:105-8. 2006
    ....
  28. ncbi Major transcript variants of VAV3, a new member of the VAV family of guanine nucleotide exchange factors
    T Trenkle
    Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, CA, USA
    Gene 245:139-49. 2000
    ..TGF-beta and EGF reversibly down-regulate the abundance of the VAV3. 1 transcript in HaCaT keratinocytes, representing the first observation of transcript regulation of a member of the VAV family by a growth factor...
  29. ncbi High immunogenic enterovirus 71 strain and its production using serum-free microcarrier Vero cell culture
    Chia Chyi Liu
    Institute of Biotechnology, Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan
    Vaccine 25:19-24. 2007
    ..Therefore, serum-free microcarrier culture is a valuable process for developing inactivated EV71 vaccines...
  30. ncbi The BMP/BMPR/Smad pathway directs expression of the erythroid-specific EKLF and GATA1 transcription factors during embryoid body differentiation in serum-free media
    Carrie A Adelman
    Department of Biochemistry and Molecular Biology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Development 129:539-49. 2002
    ....
  31. ncbi Regenerated bovine fetal fibroblasts support high blastocyst development following nuclear transfer
    L Liu
    Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, College Station, Texas 77843, USA
    Cloning 3:51-8. 2001
    ..Regenerated fetal fibroblast cells can be maintained up to at least passage 36 and still support development of nuclear transfer embryos to the blastocyst stage...
  32. ncbi Identification and discrimination of extracellularly active cathepsins B and L in high-invasive melanoma cells
    Anke Klose
    Department of Dermatology, University of Cologne, Germany
    Anal Biochem 353:57-62. 2006
    ....
  33. ncbi Nonhuman sialic acid Neu5Gc is very low in human embryonic stem cell-derived neural precursors differentiated with B27/N2 and noggin: implications for transplantation
    Igor O Nasonkin
    Department of Pathology, Division of Neuropathology, Baltimore, MD 21205, USA
    Exp Neurol 201:525-9. 2006
    ..We find this concern has little relevance to neural differentiation protocols with B27/N2/noggin because of the gradual decline of Neu5Gc to less than 1% in differentiating cells upon switching to B27/N2 medium...
  34. pmc Hypoxia-inducible factor-1alpha mRNA contains an internal ribosome entry site that allows efficient translation during normoxia and hypoxia
    Kenneth J D Lang
    Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, Adelaide, South Australia 5000, Australia
    Mol Biol Cell 13:1792-801. 2002
    ..These data indicate that the presence of an IRES in the HIF-1alpha 5'UTR allows translation to be maintained under conditions that are inhibitory to cap-dependent translation...
  35. ncbi Neural differentiation of mouse embryonic stem cells in chemically defined medium
    Isabelle A Bouhon
    Neurobiology Programme, The Babraham Institute, Babraham, Cambridge CB2 4AT, UK
    Brain Res Bull 68:62-75. 2005
    ..Taken together the profile of gene expression changes seen in CDM cultures recapitulates those seen in the early embryo, and is suggestive of common developmental mechanisms...
  36. ncbi Serum deprivation induces apoptotic cell death of transformed rat retinal ganglion cells via mitochondrial signaling pathways
    Irma Charles
    Department of Cell Biology and Genetics, University of North Texas Health Science Center, Fort Worth, Texas, USA
    Invest Ophthalmol Vis Sci 46:1330-8. 2005
    ..Apoptosis-related signaling pathways were investigated in a cultured rat retinal ganglion cell (RGC-5) line deprived of growth factors after serum withdrawal from the culture medium...
  37. ncbi Human corneal endothelial cell precursors isolated by sphere-forming assay
    Seiichi Yokoo
    Department of Corneal Tissue Regeneration, Tokyo University Graduate School of Medicine, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8655, Japan
    Invest Ophthalmol Vis Sci 46:1626-31. 2005
    ..To isolate precursors of human corneal endothelial cells (HCECs) in vitro...
  38. ncbi Derivation of human embryonic stem cell lines in serum replacement medium using postnatal human fibroblasts as feeder cells
    Jose Inzunza
    Department of Clinical Sciences, Division of Obstetrics and Gynecology, Karolinska Institutet, Karolinska University Hospital, Huddinge, S 141 86 Stockholm, Sweden
    Stem Cells 23:544-9. 2005
    ..We conclude that hESC lines can be successfully derived using SR medium and postnatal human fibroblasts as feeder cells. This is a step toward xeno-free conditions and facilitates the use of these cells in transplantation...
  39. ncbi Development of serum-free media for cell growth and production of viruses/viral vaccines--safety issues of animal products used in serum-free media
    O W Merten
    Genethon III, Evry, France
    Dev Biol (Basel) 111:233-57. 2002
    ....
  40. ncbi Transient gene expression in suspension HEK-293 cells: application to large-scale protein production
    Lucia Baldi
    Laboratory of Cellular Biotechnology, Institute for Biological Engineering and Biotechhnology, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Biotechnol Prog 21:148-53. 2005
    ..The work presented here proves the applicability and robustness of transient transfection technology for the expression of a variety of recombinant proteins...
  41. ncbi Differences in the glycosylation profile of a monoclonal antibody produced by hybridomas cultured in serum-supplemented, serum-free or chemically defined media
    J Antonio Serrato
    Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos, CP, Mexico
    Biotechnol Appl Biochem 47:113-24. 2007
    ..Approxi. 80% of potential mAb glycosylation sites were occupied, regardless of the culture medium used...
  42. ncbi Enhanced human thrombopoietin production by sodium butyrate addition to serum-free suspension culture of bcl-2-overexpressing CHO cells
    Yun Hee Sung
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373 1 Kusong Dong, Yusong gu, Daejon 305 701, Korea
    Biotechnol Prog 21:50-7. 2005
    ..Taken together, the data obtained suggest that Bcl-2 overexpression in rCHO cells and NaBu addition in serum-free suspension culture can be an effective means to enhance the production of highly glycosylated protein such as hTPO...
  43. ncbi A two-way interaction between hepatocyte growth factor and interleukin-6 in tissue invasion of lung cancer cell line
    Yasuo To
    Pulmonary Section, Department of Allergy and Rheumatology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
    Am J Respir Cell Mol Biol 27:220-6. 2002
    ..They also suggest an "autocrine circuit" among cytokines and growth factors in certain cancer cells which functions to accelerate their biologic activities such as metastatic property...
  44. pmc Rb is required for progression through myogenic differentiation but not maintenance of terminal differentiation
    Michael S Huh
    Molecular Medicine Program, Ottawa Health Research Institute, Ottawa, Ontario, Canada, K1H 8L6
    J Cell Biol 166:865-76. 2004
    ..Therefore, Rb plays a crucial role in the switch from proliferation to differentiation rather than maintenance of the terminally differentiated state...
  45. ncbi Production of calves from G1 fibroblasts
    P Kasinathan
    Department of Veterinary and Animal Science, University of Massachusetts, Amherst, MA 01003, USA
    Nat Biotechnol 19:1176-8. 2001
    ..These results indicate that donor cell cycle stage is important for NT, particularly during late fetal development, and that actively dividing G1 cells support higher development rates than cells in G0...
  46. pmc Constitutively activated Stat3 protects fibroblasts from serum withdrawal and UV-induced apoptosis and antagonizes the proapoptotic effects of activated Stat1
    Y Shen
    Laboratory of Molecular Cell Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 98:1543-8. 2001
    ..Me180 cells that express Stat3-C are protected against IFN gamma-mediated apoptosis...
  47. ncbi Insulin-like growth factor-I and transferrin mediate growth and survival of Chinese hamster ovary cells
    N A Sunstrom
    Cooperative Research Centre for Biopharmaceutical Research and Department of Biotechnology, University of New South Wales, Sydney, Australia
    Biotechnol Prog 16:698-702. 2000
    ..We report on the dual role of IGF-I as a survival factor in CHO cells and its interlocking role with transferrin to stimulate cell growth...
  48. ncbi In vitro development of porcine nuclear transfer embryos constructed using fetal fibroblasts
    P J Verma
    BresaGen Limited, Adelaide, South Australia, Australia
    Mol Reprod Dev 57:262-9. 2000
    ..Seven recipients farrowed a total of 51 piglets. Microsatellite analysis revealed that none of these were derived from the nuclear transfer embryos transferred...
  49. ncbi Characterization of a transformed rat retinal ganglion cell line
    R R Krishnamoorthy
    Department of Pathology and Anatomy, UNT Health Science Center, 3500 Camp Bowie Blvd, Fort Worth, TX 76107, USA
    Brain Res Mol Brain Res 86:1-12. 2001
    ..These cells may be valuable in understanding of retinal ganglion cell biology and physiology including in vitro manipulations in experimental models of glaucoma...
  50. ncbi Human corneal epithelial equivalents for ocular surface reconstruction in a complete serum-free culture system without unknown factors
    Seiichi Yokoo
    Departments of Corneal Tissue Regeneration, University of Tokyo Graduate School of Medicine, Tokyo, Japan
    Invest Ophthalmol Vis Sci 49:2438-43. 2008
    ....
  51. ncbi Effect of serum starvation and chemical inhibitors on cell cycle synchronization of canine dermal fibroblasts
    R Khammanit
    Department of Anatomy, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand
    Theriogenology 70:27-34. 2008
    ..In conclusion, canine dermal fibroblasts were effectively synchronized at various stages of the cell cycle, which could have benefits for somatic cell nuclear transfer in this species...
  52. ncbi Serum-free culture medium and IL-7 costimulation increase the sensitivity of ELISpot detection
    Emanuela Martinuzzi
    INSERM, U580, Necker Hospital, Paris, France
    J Immunol Methods 333:61-70. 2008
    ..Implementation of this ELISpot procedure should expedite development of "immune staging" protocols for autoimmune as well as tumor and infectious diseases...
  53. ncbi Class I histone deacetylases sequentially interact with MyoD and pRb during skeletal myogenesis
    P L Puri
    Department of Biology, University of California San Diego, La Jolla, CA 92093, USA
    Mol Cell 8:885-97. 2001
    ..These results suggest that reduced expression of HDAC1 accompanied by its redistribution in alternative nuclear protein complexes is critical for terminal differentiation of skeletal muscle cells...
  54. ncbi Suspension culture combined with anticancer regimens for screening breast cancer stem cells
    Hai zhi Li
    Department of General Surgery, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, People s Republic of China
    Med Hypotheses 68:988-90. 2007
    ..This double sorting method by means of the unique properties of BrCSCs may be helpful to screen genuine cancer stem cells(CSCs)...
  55. ncbi Neurogenic neuroepithelial and radial glial cells generated from six human embryonic stem cell lines in serum-free suspension and adherent cultures
    Roxana Nat
    Department of Neurobiology, Care Sciences and Society, Karolinska Institutet, Karolinska University Hospital Huddinge, Huddinge, Sweden
    Glia 55:385-99. 2007
    ..Our system successfully generates human RG cells and could be an effective source for neuronal replacement, since RG cells predominantly generate neurons and provide them with support and guidance...
  56. ncbi Dexamethasone prevents impairment of endothelium-dependent relaxation in arteries cultured with fetal bovine serum
    Takahisa Murata
    Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Yayoi 1 1 1, Bunkyo ku, Tokyo 113 8675, Japan
    Eur J Pharmacol 515:134-41. 2005
    ....
  57. ncbi Approaches to the release of a master cell bank of PER.C6 cells; a novel cell substrate for the manufacture of human vaccines
    J A Lewis
    Department of Live Virus Vectors, Merck Research Laboratories, Merck and Co, West Point, PA 19486, USA
    Dev Biol (Basel) 123:165-76; discussion 183-97. 2006
    ..The impact of this breadth of testing on the continued reliance of classical in vivo and in vitro assays for adventitious viruses is clearly an emerging issue worthy of serious debate...
  58. ncbi Application of a serum-free medium for the growth of Vero cells and the production of reovirus
    M Butler
    Departments of Microbiology and Medical Microbiology, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada
    Biotechnol Prog 16:854-8. 2000
    ..The high viral productivity suggests that this is a suitable system for the production of reovirus as an oncolytic agent for human therapeutic use...
  59. ncbi Identification of selenoprotein P fragments as a cell-death inhibitory factor
    Masaki Hirashima
    The Chemo Sero Therapeutic Research Institute, Kawabe, Kyokushi, Kikuchi gun, Kumamoto 869 1298, Japan
    Biol Pharm Bull 26:794-8. 2003
    ..The specific activity (unit per pmol of selenium) of selenoprotein P fragments protein was 15-fold and 1900-fold higher than that of the full-length SeP and sodium selenite, respectively...
  60. ncbi Differentiation of human embryonic stem cells to dopaminergic neurons in serum-free suspension culture
    Thomas C Schulz
    BresaGen Inc, 111 Riverbend Rd, Athens, Georgia, 30605, USA
    Stem Cells 22:1218-38. 2004
    ..This work suggests that hESCs can differentiate in simple serum-free suspension cultures to produce the large number of cells required for transplantation studies...
  61. ncbi Reappraisal of serum starvation, the restriction point, G0, and G1 phase arrest points
    Stephen Cooper
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 0620, USA
    FASEB J 17:333-40. 2003
    ..It is proposed that the arrest of cells with a particular DNA content equivalent to that in cells in the G1 phase of the division cycle does not mean there is any particular G1 phase control point...
  62. pmc Fibroblast growth factor 2-mediated translational control of IAPs blocks mitochondrial release of Smac/DIABLO and apoptosis in small cell lung cancer cells
    Olivier E Pardo
    Lung Cancer Biology Group, Cancer Research UK, London SW3, United Kingdom
    Mol Cell Biol 23:7600-10. 2003
    ....
  63. ncbi Effect of serum starvation on expression and phosphorylation of PKC-alpha and p53 in V79 cells: implications for cell death
    N M Hasan
    Department of Biology and Biochemistry, Birzeit University, West Bank, Israel
    Int J Cancer 80:400-5. 1999
    ..These data highlight the association of PKC-alpha and p53 with cellular events leading to cell death...
  64. ncbi Ultra-structural characteristics of bovine granulosa cells associated with maintenance of oestradiol production in vitro
    C G Gutierrez
    Roslin Institute Edinburgh, Midlothian, UK
    Mol Cell Endocrinol 134:51-8. 1997
    ....
  65. ncbi Latent membrane protein 1 of Epstein-Barr virus plays an important role in the serum starvation resistance of Epstein-Barr virus-immortalized B lymphocytes
    Chan Hee Park
    Division of Biological Sciences and the Institute for Molecular Biology and Genetics, Chonbuk National University, Chonju 561 756, Korea
    J Cell Biochem 91:777-85. 2004
    ....
  66. ncbi Long-term expression of differentiated functions in hepatocytes cultured in three-dimensional collagen matrix
    M J Gomez-Lechon
    Unidad de Hepatologia Experimental, Centro de Investigacion, Hospital La Fe, Valencia, Spain
    J Cell Physiol 177:553-62. 1998
    ....
  67. ncbi Development of bovine oocytes reconstructed with different donor somatic cells with or without serum starvation
    J K Cho
    Department of Theriogenology, College of Veterinary Medicine, Seoul National University, South Korea
    Theriogenology 57:1819-28. 2002
    ..3%). In conclusion, serum starvation is effective for improving preimplantation development of oocytes reconstructed with cumulus or ear fibroblast cells and it may positively influence on obtaining better pregnancy outcome...
  68. pmc Generation and maturation of bone marrow-derived DCs under serum-free conditions
    Sung Jung Kim
    Department of Autoimmune Disease, The Feinstein Institute for Medical Research, North Shore LIJ Healthy System, 350 Community Drive, Manhasset, NY 11030, United States
    J Immunol Methods 323:101-8. 2007
    ..Furthermore, DCs derived under serum-free conditions may express unusual surface markers, B220 and Ly6C/G, implying an increased differentiation to plasmacytoid DCs (pDCs)...
  69. ncbi DNA hypomethylation of karyoplasts for bovine nuclear transplantation
    K L Jones
    Department of Veterinary Physiology and Pharmacology, Texas A and M University, College Station, Texas, USA
    Mol Reprod Dev 60:208-13. 2001
    ..Supplementation of the donor cell culture medium with 5-aza-CR was not beneficial for increasing blastocyst rate or establishing pregnancy after NT...
  70. ncbi The CD34(+)CD38(neg) population is significantly increased in haemopoietic cell expansion cultures in serum-free compared to serum-replete conditions: dissociation of phenotype and function
    C Donaldson
    University of Bristol, Division of Transplantation Sciences, Southmead Hospital, Bristol, UK
    Bone Marrow Transplant 27:365-71. 2001
    ..These findings must be considered in clinical studies using in vitro expansion in SF conditions, and the CD34(+)CD38(neg) phenotype should not be used to confirm maintenance, or expansion, of primitive progenitor cells...
  71. ncbi The Rb-family protein p107 inhibits translation by a PDK1-dependent mechanism
    Constantin Makris
    Institut de Recherches Cliniques de Montréal and Department of Pharmacology, University of Montreal, 110 Pine Avenue West, Montreal, Quebec H2W 1R7, Canada
    Oncogene 21:7891-6. 2002
    ..Overexpression of PDK1 restores the defect in translation elicited by p107. These results suggest that p107 restricts cell growth by interfering with the phosphoinositide 3-kinase (PI3K) signaling pathway...
  72. pmc Common regulation of growth arrest and differentiation of osteoblasts by helix-loop-helix factors
    N Funato
    Human Gene Sciences Center, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8510, Japan
    Mol Cell Biol 21:7416-28. 2001
    ..Our results also provide the molecular basis of Saethre-Chotzen syndrome, caused by mutations of the TWIST and FGFR3 genes...
  73. ncbi Effect of antiestrogens and aromatase inhibitor on basal growth of the human breast cancer cell line MCF-7 in serum-free medium
    Janne Jensen
    Department of Tumor Endocrinology, Institute of Cancer Biology, Danish Cancer Society, Strandboulevarden 49, DK 2100 Copenhagen, Denmark
    J Steroid Biochem Mol Biol 84:469-78. 2003
    ....
  74. ncbi BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3
    Qi Long Ying
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, EH9 3JQ, Edinburgh, Scotland
    Cell 115:281-92. 2003
    ..Upon LIF withdrawal, Id-expressing ES cells differentiate but do not give rise to neural lineages. We conclude that blockade of lineage-specific transcription factors by Id proteins enables the self-renewal response to LIF/STAT3...
  75. ncbi Maintenance and self-renewal of long-term reconstituting hematopoietic stem cells supported by amniotic fluid
    Emily Barria
    Department of Biology Cancer Center, University of California, San Diego, La Jolla, CA 92093 0063, USA
    Stem Cells Dev 13:548-62. 2004
    ....
  76. ncbi Similar pattern in cardiac differentiation of human embryonic stem cell lines, BG01V and ReliCellhES1, under low serum concentration supplemented with bone morphogenetic protein-2
    Rajarshi Pal
    Embryonic Stem Cell Group, Reliance Life Sciences Ltd, Dhirubhai Ambani Life Sciences Center, South Block, R 282, TTC Industrial Area of MIDC, Thane Belapur Road, Rabale, Navi Mumbai 400 701, India
    Differentiation 75:112-22. 2007
    ..The present data may also provide valuable insights for testing of other factors that might promote cardiomyocyte differentiation in low-serum formulations...
  77. pmc The forkhead-associated domain protein Cep170 interacts with Polo-like kinase 1 and serves as a marker for mature centrioles
    Giulia Guarguaglini
    Department of Cell Biology, Max Planck Institute for Biochemistry, D 82152, Martinsried, Germany
    Mol Biol Cell 16:1095-107. 2005
    ..We show that Cep170 labeling can be used to discriminate bona fide centriole overduplication from centriole amplification that results from aborted cell division...
  78. ncbi Effect of amyloid peptides on serum withdrawal-induced cell differentiation and cell viability
    Yi Peng Wang
    Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
    Cell Res 14:467-72. 2004
    ..These results suggest that the overproduction of A beta could not arrest cell differentiation induced by serum deprivation and that, at least to a certain degree and in a limited time period, is not toxic to cell viability...
  79. ncbi Human placenta and bone marrow derived MSC cultured in serum-free, b-FGF-containing medium express cell surface frizzled-9 and SSEA-4 and give rise to multilineage differentiation
    Venkata Lokesh Battula
    Department of Internal Medicine II, Division of Hematology, Immunology, Oncology and Rheumatology, University Clinic of Tubingen, Tubingen, Germany
    Differentiation 75:279-91. 2007
    ..In conclusion, using a novel protocol we demonstrate that adult BM-and neonatal PL-derived MSC can be induced to express high levels of FZD-9, Oct-4, nanog-3, and nestin and are able of multi-lineage differentiation...
  80. ncbi Initiation of caspase-independent death in mouse mesangial cells by Cd2+: involvement of p38 kinase and CaMK-II
    Ying Liu
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada
    J Cell Physiol 217:307-18. 2008
    ..Necrosis is not prominent. Activation of p38 kinase and of CaMK-II by Cd(2+) are associated with caspase-independent apoptosis that is not dependent on mitochondrial destabilization...
  81. pmc Hydrophobic surfaces for enhanced differentiation of embryonic stem cell-derived embryoid bodies
    Bahram Valamehr
    Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 105:14459-64. 2008
    ..Our data demonstrate that hydrophobic surfaces serve as a platform to deliver uniform EB populations and may significantly improve the efficiency of ES cell differentiation...
  82. ncbi Integrin-linked kinase is a positive mediator of L6 myoblast differentiation
    Mathew G Miller
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Ont, Canada
    Biochem Biophys Res Commun 310:796-803. 2003
    ..Our data do suggest that a LiCl-sensitive interaction of ILK is important in L6 myoblast differentiation...
  83. ncbi Isolation and culture of preadipocytes from rodent white adipose tissue
    Dorothy B Hausman
    Department of Foods and Nutrition, University of Georgia, Athens, GA, USA
    Methods Mol Biol 456:201-19. 2008
    ..Alternatively, preadipocytes differentiated in vitro may be used for examining adipocyte metabolic or secretory responses...
  84. ncbi The effect of hypoxia and stem cell source on haemoglobin switching
    A Daisy Narayan
    Department of Animal Biotechnology, University of Nevada, Reno, NV 89557, USA
    Br J Haematol 128:562-70. 2005
    ..The erythroid-specific isoform of 5-amino-levulinate synthase (ALAS2) was also expressed under hypoxic conditions. We conclude that Hb switching is affected by the environment but not all HSCs are preprogrammed to respond...
  85. ncbi Insulin-like growth factor binding protein (IGFBP)-5 is upregulated during both differentiation and apoptosis in primary cultures of mouse mammary epithelial cells
    Jennifer D Lochrie
    Molecular Recognition Group, Hannah Research Institute, Ayr, Scotland
    J Cell Physiol 207:471-9. 2006
    ..We discuss the possible implications of our observations in relation to the physiological processes of pregnancy, lactation, and involution in the mammary gland and the associated changes in mammary epithelial cell function...
  86. pmc Mechanisms regulating plasminogen activators in transformed retinal ganglion cells
    Nathan Rock
    Eye Research Institute, Oakland University, Rochester, MI 48309, USA
    Exp Eye Res 86:492-9. 2008
    ..These results indicate that LRP-1 regulates autocrine synthesis of tPA and uPA in RGC-5 cells and suggest that the use of RAP to antagonize the effect of proteases may be a way to prevent RGC death in glaucoma...
  87. ncbi Generation of bone marrow-derived neural cells in serum-free monolayer culture
    Takuro Magaki
    Department of Neurosurgery, Graduate School of Biomedical Sciences, Hiroshima University, 1 2 3 Kasumi, Minami Ku, Hiroshima 734 8551, Japan
    Neurosci Lett 384:282-7. 2005
    ..Intracerebrally transplanted BMCs survived and migrated into the subcortex of syngenic animals...
  88. ncbi Increased cardiomyocyte differentiation from human embryonic stem cells in serum-free cultures
    Robert Passier
    Hubrecht Laboratory, Uppsalalaan 8, 3584 CT Utrecht, Netherlands
    Stem Cells 23:772-80. 2005
    ..The present data provide a step in this direction and represent an improved in vitro model, without interfering factors in serum, for testing other factors that might promote cardiomyocyte differentiation...
  89. ncbi Long-term platelet production assessed in NOD/SCID mice injected with cord blood CD34+ cells, thrombopoietin-amplified in clinical grade serum-free culture
    Gianfranco Mattia
    Department of Hematology, Oncology and Molecular Medicine, Section of Transfusion Methodologies, Istituto Superiore di Sanita, Rome, Italy
    Exp Hematol 36:244-52. 2008
    ..This study assesses the hypothesis that CB-CD34+ amplification with thrombopoietin (TPO) can be applied to a portion of a CB transplant unit to stimulate recovery along MK differentiation program...
  90. ncbi Serum-free culture conditions for serial subculture of undifferentiated PC12 cells
    Kiyoshi Ohnuma
    Department of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo, 3 8 1 Komaba, Meguro, Tokyo 153 8902, Japan
    J Neurosci Methods 151:250-61. 2006
    ....
  91. pmc Hepatocyte growth factor receptor signaling mediates the anti-fibrotic action of 9-cis-retinoic acid in glomerular mesangial cells
    Xiaoyan Wen
    Department of Pathology, University of Pittsburgh School of Medicine, S 405 Biomedical Science Tower, 200 Lothrop St, Pittsburgh, PA 15261, USA
    Am J Pathol 167:947-57. 2005
    ..Collectively, these results indicate that 9-cis-RA possesses anti-fibrotic ability by antagonizing TGF-beta1 in mesangial cells and that 9-cis-RA activity is likely mediated through a mechanism dependent on HGF/c-met receptor signaling...
  92. ncbi A xenobiotic-free culture system for human limbal epithelial stem cells
    Maria Notara
    UCL Institute of Ophthalmology, Cells for Sight Transplantation and Research Programme, Ocular Repair and Regeneration Biology Unit, 11 43 Bath Street, London, EC1V 9EL, UK
    Regen Med 2:919-27. 2007
    ....
  93. ncbi Modeling trophoblast differentiation using equine chorionic girdle vesicles
    A M de Mestre
    Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Placenta 29:158-69. 2008
    ....
  94. ncbi Effect of neural stem cells on apoptosis of PC12 cells induced by serum deprivation
    Xiangqin Li
    Dalain R and D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China
    Biotechnol Prog 23:952-7. 2007
    ..It can be concluded that there exists clear interaction between NSCs and apoptotic PC12 cells, and that GDNF secretion from NSCs is one of the important mechanisms to prevent the apoptosis of PC12 cells...
  95. ncbi Wnt proteins prevent apoptosis of both uncommitted osteoblast progenitors and differentiated osteoblasts by beta-catenin-dependent and -independent signaling cascades involving Src/ERK and phosphatidylinositol 3-kinase/AKT
    Maria Almeida
    Division of Endocrinology and Metabolism, Center for Osteoporosis and Metabolic Bone Diseases, University of Arkansas for Medical Sciences and the Central Arkansas Veterans Health Care System, Little Rock, Arkansas 72205, USA
    J Biol Chem 280:41342-51. 2005
    ....
  96. ncbi Survival and stimulation of neurite outgrowth in a serum-free culture of spiral ganglion neurons from adult mice
    Mauricio Vieira
    Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana Champaign, MC 251, Urbana, IL, USA
    Hear Res 230:17-23. 2007
    ..This responsiveness to exogenous growth factors suggested that our culture protocol will facilitate the screening of molecular compounds as potential treatments for sensorineural hearing loss...
  97. ncbi Exogenous hydrogen sulfide induces functional inhibition and cell death of cytotoxic lymphocytes subsets
    Prisco Mirandola
    Department of Anatomy, Pharmacology and Forensic Medicine, Human Anatomy Section, University of Parma, Ospedale Maggiore, Via Gramsci, Parma, Italy
    J Cell Physiol 213:826-33. 2007
    ....
  98. ncbi Closed system generation of dendritic cells from a single blood volume for clinical application in immunotherapy
    M Elias
    Sanquin Division North East, GG Groningen, The Netherlands
    J Clin Apher 20:197-207. 2005
    ....
  99. ncbi Increased malignant behavior in neuroblastoma cells with acquired multi-drug resistance does not depend on P-gp expression
    Rouslan Kotchetkov
    Interdisziplinäres Laboratorium für Tumor und Virus Forschung, Institut fur Medizinische Virologie, Clinics of J W Goethe University, Frankfurt am Main, Germany
    Int J Oncol 27:1029-37. 2005
    ..We conclude that acquisition of increased malignant behavior in neuroblastoma occurs concomitantly with multidrug-resistance and is P-gp-independent...
  100. ncbi Haem oxygenase-1 prevents cell death by regulating cellular iron
    C D Ferris
    Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Nat Cell Biol 1:152-7. 1999
    ..Thus, cytoprotection by HO1 is attributable to its augmentation of iron efflux, reflecting a role for HO1 in modulating intracellular iron levels and regulating cell viability...
  101. ncbi Proliferation and differentiation of bovine type A spermatogonia during long-term culture
    Fariborz Izadyar
    Department of Endocrinology, Faculty of Biology, University Medical Center Utrecht, 3548 CH Utrecht, The Netherlands
    Biol Reprod 68:272-81. 2003
    ..For the first time to our knowledge, a method has been developed to allow proliferation and differentiation of highly purified type A spermatogonia, including spermatogonial stem cells during long-term culture...

Research Grants65

  1. Thiol-Ene Click Hydrogels for in situ Cell Expansion and Differentiation
    Chien Chi Lin; Fiscal Year: 2012
    ..Together, our strategies facilitate the formation of islet-like cell clusters with natural cell-cell interactions and normal insulin secretion profiles. ..
  2. DIFFERENTIATED CELLS AS TARGETS FOR CARCINOGEN-SCREENING
    P Iype; Fiscal Year: 1992
    ..Procedures and reagents developed will be useful in screening environmental chemicals for their carcinogenic potential, hence commercially valuable...
  3. Regulation of Oocyte Viability by Granulosa Cell Contact
    John Peluso; Fiscal Year: 2004
    ..abstract_text> ..
  4. Age-Dependent Response of Hippocampal Neurons to Stress
    Gregory Brewer; Fiscal Year: 2006
    ..abstract_text> ..
  5. Protein Kinase G Regulation of Granulosa Cell Viability
    John Peluso; Fiscal Year: 2006
    ..We now propose to test this hypothesis using biochemical, genetic, imaging and cell biological techniques. [unreadable] [unreadable]..
  6. Progesterone regulation of human luteal cell viability
    John Peluso; Fiscal Year: 2006
    ..Such selective manipulation of the P4's actions could have far reaching effects on the treatment for infertility, contraception and certain types of cancers. [unreadable] [unreadable]..
  7. ROLE OF THE FAS ANTIGEN IN OVARIAN CELL APOPTOSIS
    SUSAN QUIRK; Fiscal Year: 2004
    ..Understanding pathways that promote or prevent susceptibility of ovarian cells to apoptosis may lead to development of novel therapies for ovarian and other cancers. ..
  8. Development of neuromuscular junctions in children with cerebral palsy
    Robert Akins; Fiscal Year: 2008
    ..The PIs study these interactions in order to develop therapies and treatments that will improve and sustain the physical ability and health of patients with CP. [unreadable] [unreadable] [unreadable]..
  9. The Molecular Basis of Ovarian Testosterone Production
    Robert Rosenfield; Fiscal Year: 2004
    ..The overall goal of this proposal is to understand the factors regulating the production of testosterone in women, particularly in their ovaries. ..
  10. HGF Gene Therapy for Chronic Renal Fibrosis
    Youhua Liu; Fiscal Year: 2009
    ..Ultimately, these studies may lead to development of novel strategies to alter the course of human kidney disease by manipulating the activity of HGF signaling system. ..
  11. Regulation of the pro-apoptotic protein Bax during neuronal apoptosis
    Mika Jekabsons; Fiscal Year: 2008
    ..Data obtained from this proposal will contribute to understanding the mechanisms responsible for neuronal apoptosis, thus aiding drug development to limit the severity of these pathologies. [unreadable] [unreadable]..
  12. Molecular Mechanisms Regulating Stem Cell Plasticity
    Michael Rudnicki; Fiscal Year: 2004
    ..To investigate the mechanism by which Pax7 enforces lineage commitment, FACS/Hoechst enriched adult stem cells from Pax7-/- mice will be transplanted either intravenously or intramuscularly. ..
  13. Gene Delivery to the Trabecular Meshwork
    Douglas Rhee; Fiscal Year: 2004
    ..These studies would set the foundation for future evaluations of the function of MIF in the TM and MIF?s potential as a candidate for gene therapy for the treatment of glaucoma. ..
  14. ROLE OF IGF-I IN PREVENTING CARDIOMYOCYTE APOPTOSIS
    Wei Chao; Fiscal Year: 2005
    ..abstract_text> ..
  15. "Ligand Independent" endocrine disruption by pesticides
    Patrick Wong; Fiscal Year: 2005
    ..abstract_text> ..
  16. Cathepsin B and cystatin C in AD-related neuronal damage
    Li Gan; Fiscal Year: 2005
    ..These studies will shed light on the potential role of cysC in AD pathogenesis and further our understanding on the CatB-mediated neuronal damage in AD. ..
  17. Mechanisms of VEGF Action in Intact and Grafted CNS
    JEFFREY ROSENSTEIN; Fiscal Year: 2006
    ..These studies may provide impetus for the further design strategies for the circumvention of the BBB and/or the targeting of VEGF receptor mediated effects in brain injury. [unreadable] [unreadable]..
  18. Engineered neuronal networks for drug screening
    Peter Molnar; Fiscal Year: 2008
    ..abstract_text> ..
  19. Two-step therapeutic screen for mitochondrial epilepsies
    Manisha Patel; Fiscal Year: 2007
    ..This screening procedure will allow us to participate the the NINDS cooperative program designed to specifically develop candidate therapies for clinical development. [unreadable] [unreadable] [unreadable]..
  20. PHOSPHATIDYLSERINE-SPECIFIC RECEPTOR FOR APOPTOTIC CELLS
    Valerie Fadok; Fiscal Year: 2004
    ..The investigators hypothesize that PS recognition has been preserved throughout phylogeny as an effective mechanism mediating the removal of apoantibodies. ..
  21. Green tea derived EGCG opposes AIDS dementia-like neuronal damage
    Jun Tan; Fiscal Year: 2008
    ..These data should lay the foundation for further research leading to human trials in the near future. [unreadable] [unreadable] [unreadable]..
  22. HGF RECEPTOR GENE REGULATION AND RENAL GROWTH
    Youhua Liu; Fiscal Year: 2003
    ..Ultimately, these studies may suggest novel strategies to alter the course of human renal disease by regulating the HGF/c-met axis. ..
  23. PROTEIN SYNTHESIS INITIATION IN ANIMAL CELLS
    Bansidhar Datta; Fiscal Year: 2002
    ..This research is expected to provide valuable information regarding the mechanism of protein synthesis initiation in normal, cancerous, and virus- infected animal cells. ..
  24. ENGINEERING INTEGRIN--MEDIATED CELL MIGRATION
    Douglas Lauffenburger; Fiscal Year: 2002
    ..The knowledge gained from these studies may help to control the cell migration in tissue engineering applications. ..
  25. SQUAMOUS EPITHELIAL CELL INVASION IN ORGAN CULTURE
    James Varani; Fiscal Year: 2001
    ..abstract_text> ..
  26. FREE PLASMA SEX HORMONE AND ANDROGEN ACTION
    Robert Rosenfield; Fiscal Year: 2001
    ....
  27. Molecular Mechanisms That Regulate CD11d Expression
    JOHN NOTI; Fiscal Year: 2004
    ..Transgenic analysis of the CD11d cis elements will be done to complement these studies. These studies will expand our understanding of normal myeloid development and leukemogenesis. ..
  28. Regulation of NF-kB by the Akt kinase
    Lawrence P Kane; Fiscal Year: 2010
    ..Such knowledge may eventually lead to more specific and efficacious treatments for tumors with dysregulated Akt. ..
  29. REGULATION OF CHONDROCYTE MATURATION
    PHOEBE LEBOY; Fiscal Year: 2001
    ..3) To examine the relationship between BMP-mediated MMP13 expression and matrix mineralization. ..
  30. Serum Proteins Influencing Helicobacter pylori Growth
    TRACI TESTERMAN; Fiscal Year: 2007
    ..A more complete understanding of serum proteins interacting with bacteria could lead to advancements in cultivation of other organisms as well. ..
  31. Role of p53 in Osteoblast Differentiation
    Nalini Chandar; Fiscal Year: 2003
    ..abstract_text> ..
  32. STEM-LIKE CELLS AND TUMORGENICITY IN OSTEOSARCOMA
    CHARLES JR GIBBS; Fiscal Year: 2007
    ..demonstration of the contribution of stem-like cells in the tumorigenesis of osteosarcomas and provide a foundation upon which to base large scale investigations suitable for an R01 level application [unreadable] [unreadable] [unreadable]..
  33. STUDIES OF THE CORNEA
    William Bourne; Fiscal Year: 2007
    ..Knowledge of these effects will lead to improvements in these surgical procedures. ..
  34. Regulation of the p53 gene during osteoblast differentiation.
    Nalini Chandar; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  35. The Role of BCL-2 in Corneal Epithelial Cell Shedding
    DANIELLE ROBERTSON; Fiscal Year: 2008
    ..4) That events leading to increased apoptosis such as UV light signal surface cell apoptosis through the phosphorylation of BCL-2. ..
  36. Reprogramming of X-linked genes by nuclear transfer
    X Tian; Fiscal Year: 2003
    ..abstract_text> ..
  37. ENDOTHELIAL CELL ROLE IN YOLK SAC STEM CELL DEVELOPMENT
    Mervin Yoder; Fiscal Year: 2008
    ..These studies will define molecular pathways required for blood island development and remodeling. ..
  38. HUCBC modulation of Alzheimer-like pathology and behavioral changes
    Jun Tan; Fiscal Year: 2007
    ..This knowledge is used to rationalize U-CORD-CELL" as a potentially safe and effective immunomodulatory therapy for AD. [unreadable] [unreadable] [unreadable]..
  39. Role of IGFBP -3 in mediating p53-induced apoptosis
    Adda Grimberg; Fiscal Year: 2006
    ..These studies also reflect Dr. Grimberg?s long-term career goals of exploring the interrelatedness of endocrine and oncologic molecular biology principles and applying them to clinical problems of dysregulated growth. ..
  40. OSTEOGENIC INDUCTION WITH IMPLANT MATERIALS
    PHOEBE LEBOY; Fiscal Year: 2003
    ..abstract_text> ..
  41. Metastasis Competent Genes in Lung Cancer
    Alan Lader; Fiscal Year: 2003
    ..Lader (the PI), Dr. Kwiatkowski, an expert in genetics and molecular biology, and are an expert in metastasis. ..
  42. Effect of defined surfaces on cardiac myocyte physiology
    Peter Molnar; Fiscal Year: 2004
    ..Results obtained in this study would have a high impact on the progress of the emerging new cardiac tissue engineering methods. ..
  43. Nociception in Diabetic Neuropathy: Role of Endothelins
    Liliana Berti Mattera; Fiscal Year: 2004
    ..abstract_text> ..
  44. BMP SIGNALING DURING HUMAN BONE REPAIR
    PHOEBE LEBOY; Fiscal Year: 2004
    ..By doing so, it is hoped to provide guidance for future clinical approaches using mesenchymal stem cells in bone repair and formation. ..
  45. Expansion and Cryopreservation of Islets for Transplant
    Jonathan Carter; Fiscal Year: 2005
    ..Both aims represent the next experimental step in a logical progression towards translation if these techniques into clinical trials of human islet transplantation. ..
  46. Mechanisms of MMP-3 Action in Acute Lung Injury
    James Varani; Fiscal Year: 2005
    ..These studies will provide an overall understanding of the mechanism(s) by which MMP-3 contributes to acute lung injury. ..
  47. BIOREGULATION OF THE SPERMATOGONIAL STEM CELL LINEAGE
    LAURA TRES; Fiscal Year: 2003
    ..2. In the long range, the discovery of ways to prevent and modulate spermatogonial cell death may have significant therapeutic and anticonceptive potentials in humans. ..
  48. HGF receptor and renal cell survival and differentiation
    Youhua Liu; Fiscal Year: 2006
    ..Ultimately, these studies may lead to development of novel strategies to alter the course of human renal disease by manipulating the activities of HGF/c-met signaling system. ..
  49. ANTIBODY PROBES OF CNS MITOCHONDRIAL APOPTOTIC FUNCTION
    Ronald Jemmerson; Fiscal Year: 2004
    ..The feasibility of the proposed research is supported by preliminary results and by the participation of on-site collaborators with expertise in the field of neuroscience, in particular the physiology of CNS mitochondria. ..
  50. Cord Blood Stem Cell Expansion Ex Vivo With Endothelial Progenitor Cells
    Mervin Yoder; Fiscal Year: 2005
    ..abstract_text> ..
  51. TOPICAL RETINOIDS FOR DIABETIC FOOT ULCERS
    James Varani; Fiscal Year: 2003
    ..abstract_text> ..
  52. NEUROTROPHIC FACTOR MODULATION OF CORNEAL ENDOTHELIUM
    Shay Whey Koh; Fiscal Year: 2009
    ..abstract_text> ..
  53. ALVEOLAR TYPE II CELL GROWTH IN INJURY
    RANDOLPH HASTINGS; Fiscal Year: 2003
    ..The project will evaluate the potential use of PTHrP-related therapeutic interventions that might speed or improve recovery following silica-induced lung injury. ..
  54. CD40 Modulation of Abeta-Vaccine Immune Response
    Jun Tan; Fiscal Year: 2007
    ..abstract_text> ..
  55. Integrin-linked Kinase and Renal Interstitial Fibrosis
    Youhua Liu; Fiscal Year: 2008
    ..abstract_text> ..
  56. Effects of VEGF on Brain Tissue in vitro
    JEFFREY ROSENSTEIN; Fiscal Year: 2004
    ..The results can lay important cell biological groundwork for understanding how brain tissue might respond to VEGF-based therapy. ..
  57. Fibronectin Fragment-Induced Osteolysis Mediated by MMPs
    Sunil Kapila; Fiscal Year: 2009
    ..These studies will provide a fundamental understanding of the basis for bone loss in periodontal disease and other osteolytic diseases. ..
  58. Craniofacial Imaging in the 21st Century
    Sunil Kapila; Fiscal Year: 2002
    ..The meeting will be limited to 300 participants who have a background in imaging. This symposia series will provide momentum to discoveries that will be important to craniofacial sciences and patient care. ..
  59. 25-hydroxy-D3 as a possible chemopreventive agent for breast cancer
    Xinjian Peng; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  60. CRANIOFACIAL SKELETAL TISSUE ENGINEERING CONFERENCE
    Sunil Kapila; Fiscal Year: 2008
    ..The meeting will be limited to 250 participants who have a background in bioengineering. ..
  61. Fibrous Templates for Directed Nerve Regeneration
    JOSEPH COREY; Fiscal Year: 2008
    ..It is hoped that this project will further knowledge of both the production of advanced fibrous scaffoldings and their potential to be harnessed as a tool for nerve regeneration. ..
  62. RESPIRATORY FAILURE IN A MODEL OF P. CARINII PNEUMONIA
    Robert Paine; Fiscal Year: 2004
    ..carinii and alveolar epithelial cells through integrins induces GM-CSF expression. Finally, they will evaluate the protective effect of GM-CSF in P. carinii pneumonia and in subsequent progression of lung injury following hyperoxia. ..
  63. p38 MAP Kinase and Akt Interaction in HUVEC
    Shehla Pervin; Fiscal Year: 2008
    ..This investigation could help us understand the mechanisms by which the mortality rate of male fetus and neonates is higher than female. [unreadable] [unreadable] [unreadable]..
  64. Mineralization studies related to atherosclerosis
    HOWARD HSU; Fiscal Year: 2004
    ..abstract_text> ..
  65. Culture and Transplantation of Human Retinal Spheres
    DAVID GAMM; Fiscal Year: 2008
    ..Together, these experiments are designed to build on recent advances in the burgeoning field of stem cell biology and apply them to the basic and clinical science of ophthalmology. ..