culture media

Summary

Summary: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.

Top Publications

  1. ncbi Effects of cell culture media on the dynamic formation of protein-nanoparticle complexes and influence on the cellular response
    Gabriele Maiorano
    Italian Institute of Technology, Center for Bio Molecular Nanotechnology, Via Barsanti 73010 Arnesano, Lecce, Italy
    ACS Nano 4:7481-91. 2010
  2. ncbi Functional profiling of the Saccharomyces cerevisiae genome
    Guri Giaever
    Stanford Genome Technology Center, Stanford University, Palo Alto, California 94304, USA
    Nature 418:387-91. 2002
  3. pmc A molecular mechanism of chronological aging in yeast
    Christopher R Burtner
    Department of Biochemistry, University of Washington, 1959 NE Pacific Street, Seattle, WA 98195, USA
    Cell Cycle 8:1256-70. 2009
  4. pmc Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells
    G R Martin
    Proc Natl Acad Sci U S A 78:7634-8. 1981
  5. ncbi Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro
    N Jaiswal
    Osiris Therapeutics, Inc, Baltimore, MD 21231 2001
    J Cell Biochem 64:295-312. 1997
  6. ncbi Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture
    Qi Long Ying
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, United Kingdom
    Nat Biotechnol 21:183-6. 2003
  7. pmc Coordination of growth rate, cell cycle, stress response, and metabolic activity in yeast
    Matthew J Brauer
    Lewis Sigler Institute for Integrative Genomics and Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Mol Biol Cell 19:352-67. 2008
  8. ncbi Protein production by auto-induction in high density shaking cultures
    F William Studier
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    Protein Expr Purif 41:207-34. 2005
  9. pmc Superoxide is a mediator of an altruistic aging program in Saccharomyces cerevisiae
    Paola Fabrizio
    Andrus Gerontology Center and Dept of Biological Sciences, University of Southern California, 3715 McClintock Avenue, Los Angeles, CA 90089 0191, USA
    J Cell Biol 166:1055-67. 2004
  10. ncbi Matrigel: basement membrane matrix with biological activity
    Hynda K Kleinman
    NIH, NIDCR, 30 433, 30 Convent Dr MSC 4370, Bethesda, MD 20892 4370, USA
    Semin Cancer Biol 15:378-86. 2005

Detail Information

Publications397 found, 100 shown here

  1. ncbi Effects of cell culture media on the dynamic formation of protein-nanoparticle complexes and influence on the cellular response
    Gabriele Maiorano
    Italian Institute of Technology, Center for Bio Molecular Nanotechnology, Via Barsanti 73010 Arnesano, Lecce, Italy
    ACS Nano 4:7481-91. 2010
    ..These results show that, beyond an in-depth NPs characterization before cellular experiments, a detailed understanding of the effects elicited by cell culture media on NPs is crucial for standardized nanotoxicology tests.
  2. ncbi Functional profiling of the Saccharomyces cerevisiae genome
    Guri Giaever
    Stanford Genome Technology Center, Stanford University, Palo Alto, California 94304, USA
    Nature 418:387-91. 2002
    ..Our results validate the yeast gene-deletion collection as a valuable resource for functional genomics...
  3. pmc A molecular mechanism of chronological aging in yeast
    Christopher R Burtner
    Department of Biochemistry, University of Washington, 1959 NE Pacific Street, Seattle, WA 98195, USA
    Cell Cycle 8:1256-70. 2009
    ..We conclude that acetic acid induced mortality is the primary mechanism of chronological aging in yeast under standard conditions...
  4. pmc Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells
    G R Martin
    Proc Natl Acad Sci U S A 78:7634-8. 1981
    ..The availability of such cell lines should made possible new approaches to the study of early mammalian development...
  5. ncbi Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro
    N Jaiswal
    Osiris Therapeutics, Inc, Baltimore, MD 21231 2001
    J Cell Biochem 64:295-312. 1997
    ....
  6. ncbi Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture
    Qi Long Ying
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, United Kingdom
    Nat Biotechnol 21:183-6. 2003
    ..This system provides a platform for defining the molecular machinery of neural commitment and optimizing the efficiency of neuronal and glial cell production from pluripotent mammalian stem cells...
  7. pmc Coordination of growth rate, cell cycle, stress response, and metabolic activity in yeast
    Matthew J Brauer
    Lewis Sigler Institute for Integrative Genomics and Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Mol Biol Cell 19:352-67. 2008
    ..This concept is useful in interpreting the system-level connections among growth rate, metabolism, stress, and the cell cycle...
  8. ncbi Protein production by auto-induction in high density shaking cultures
    F William Studier
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    Protein Expr Purif 41:207-34. 2005
    ..Selenomethionine labeling was equally efficient in the commonly used methionine auxotroph B834(DE3) (found to be metE) or the prototroph BL21(DE3)...
  9. pmc Superoxide is a mediator of an altruistic aging program in Saccharomyces cerevisiae
    Paola Fabrizio
    Andrus Gerontology Center and Dept of Biological Sciences, University of Southern California, 3715 McClintock Avenue, Los Angeles, CA 90089 0191, USA
    J Cell Biol 166:1055-67. 2004
    ..The role of similar pathways in the regulation of longevity in organisms ranging from yeast to mice raises the possibility that mammals may also undergo programmed aging...
  10. ncbi Matrigel: basement membrane matrix with biological activity
    Hynda K Kleinman
    NIH, NIDCR, 30 433, 30 Convent Dr MSC 4370, Bethesda, MD 20892 4370, USA
    Semin Cancer Biol 15:378-86. 2005
    ..In vivo, it is used for measuring angiogenic inhibitors and stimulators, to improve graft survival, repair damaged tissues, and increase tumor growth...
  11. ncbi The chronological life span of Saccharomyces cerevisiae
    Paola Fabrizio
    Andrus Gerontology Center, Division of Biogerontology and Department of Biological Sciences, University of Southern California, Los Angeles 90089 0191, USA
    Aging Cell 2:73-81. 2003
    ..We will review the yeast S. cerevisiae model system with emphasis on the chronological life span as a model system to study aging and the regulation of stress resistance in eukaryotes...
  12. ncbi Human platelet lysate can replace fetal bovine serum for clinical-scale expansion of functional mesenchymal stromal cells
    Katharina Schallmoser
    Department of Blood Group Serology and Transfusion Medicine, Division of Hematology and Stem Cell Transplantation, Medical University, Auenbrugger Platz 38, A 8036 Graz, Austria
    Transfusion 47:1436-46. 2007
    ..Translation of auspicious experimental results into clinical applications has been limited by the dependence of MSC propagation from fetal bovine serum (FBS)...
  13. ncbi Recombinant vitronectin is a functionally defined substrate that supports human embryonic stem cell self-renewal via alphavbeta5 integrin
    Stefan R Braam
    Leiden University Medical Centre, Postal Zone S 1 P, P O Box 9600, 2300 RC Leiden, The Netherlands
    Stem Cells 26:2257-65. 2008
    ..Recombinant vitronectin was the only defined functional alternative to Matrigel, supporting sustained self-renewal and pluripotency in three independent hESC lines...
  14. ncbi Stochastic gene expression in a single cell
    Michael B Elowitz
    Laboratory of Cancer Biology, Center for Studies in Physics and Biology, Rockefeller University, New York, NY 10021, USA
    Science 297:1183-6. 2002
    ..These results establish a quantitative foundation for modeling noise in genetic networks and reveal how low intracellular copy numbers of molecules can fundamentally limit the precision of gene regulation...
  15. ncbi Birth of piglets derived from porcine zygotes cultured in a chemically defined medium
    Koji Yoshioka
    Department of Production Diseases, National Institute of Animal Health, Tsukuba, Ibaraki 305 0856, Japan
    Biol Reprod 66:112-9. 2002
    ..Our results indicate that porcine zygotes can develop into blastocysts in a chemically defined medium and to full term by transfer to recipients after culture...
  16. ncbi Exploring the metabolic and genetic control of gene expression on a genomic scale
    J L DeRisi
    Department of Biochemistry, Stanford University School of Medicine, Howard Hughes Medical Institute, Stanford, CA 94305 5428, USA
    Science 278:680-6. 1997
    ..These results demonstrate the feasibility and utility of this approach to genomewide exploration of gene expression patterns...
  17. ncbi Noninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded pilot study
    Richard Scott
    Reproductive Medicine Associates of New Jersey, Morristown, New Jersey, USA
    Fertil Steril 90:77-83. 2008
    ..To determine if metabolomic profiling of embryonic development was associated with implantation rates in IVF...
  18. pmc pH neutralization protects against reduction in replicative lifespan following chronological aging in yeast
    Christopher Murakami
    Department of Pathology, University of Washington Seattle, WA, USA
    Cell Cycle 11:3087-96. 2012
    ....
  19. pmc Caloric restriction or catalase inactivation extends yeast chronological lifespan by inducing H2O2 and superoxide dismutase activity
    Ana Mesquita
    Instituto de Investigação em Ciências da Vida e Saúde, Escola de Ciências da Saúde, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Proc Natl Acad Sci U S A 107:15123-8. 2010
    ..These findings establish a role for hormesis effects of hydrogen peroxide in promoting longevity that have broad implications for understanding aging and age-related diseases...
  20. ncbi Adult stem cells from bone marrow (MSCs) isolated from different strains of inbred mice vary in surface epitopes, rates of proliferation, and differentiation potential
    Alexandra Peister
    Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA 70112, USA
    Blood 103:1662-8. 2004
    ..The differences among MSCs from different strains may explain some of the conflicting data recently published on the engraftment of mouse MSCs or other bone marrow cells into nonhematopoietic tissues...
  21. pmc Two routes of metabolic cross-feeding between Bifidobacterium adolescentis and butyrate-producing anaerobes from the human gut
    Alvaro Belenguer
    Microbial Ecology Group, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, United Kingdom
    Appl Environ Microbiol 72:3593-9. 2006
    ..adolescentis and butyrate-forming bacteria may operate in gut ecosystems, one due to consumption of fermentation end products (lactate and acetate) and the other due to cross-feeding of partial breakdown products from complex substrates...
  22. ncbi PKA and Sch9 control a molecular switch important for the proper adaptation to nutrient availability
    Johnny Roosen
    Functional Biology, Katholieke Universiteit Leuven, Kasteelpark Arenberg, B 3001 Leuven Heverlee, Belgium
    Mol Microbiol 55:862-80. 2005
    ..This mechanism allows fine-tuning various nutritional responses in yeast cells, allowing them to adapt metabolism and growth appropriately...
  23. pmc Quorum sensing in the dimorphic fungus Candida albicans is mediated by farnesol
    J M Hornby
    School of Biological Sciences, University of Nebraska, Lincoln, Nebraska 68588-0666, USA
    Appl Environ Microbiol 67:2982-92. 2001
    ..albicans supernatants, i.e., 50% GTF at ca. 30 to 35 microM. Nerolidol was ca. two times less active than farnesol. Neither geraniol (C(10)), geranylgeraniol (C(20)), nor farnesyl pyrophosphate had any QSM activity...
  24. ncbi Characterization of the optimal culture conditions for clinical scale production of human mesenchymal stem cells
    Panagiota A Sotiropoulou
    Cancer Immunology and Immunotherapy Center, Saint Savas Hospital, 171 Alexandras Avenue, 115 22, Athens, Greece
    Stem Cells 24:462-71. 2006
    ..Furthermore, the use of basic fibroblast growth factor (bFGF), the most common growth supplement in MSC culture media, greatly increases the proliferation rate but also upregulates HLA-class I and induces low HLA-DR expression...
  25. pmc Electricity production by Geobacter sulfurreducens attached to electrodes
    Daniel R Bond
    Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USA
    Appl Environ Microbiol 69:1548-55. 2003
    ..sulfurreducens that can attach to electrodes and remain viable for long periods of time while completely oxidizing organic substrates with quantitative transfer of electrons to an electrode...
  26. ncbi Feeder-free growth of undifferentiated human embryonic stem cells
    C Xu
    Geron Corporation, 230 Constitution Drive, Menlo Park, CA 94025, USA
    Nat Biotechnol 19:971-4. 2001
    ..Thus, the cells retain fundamental characteristics of hES cells in this culture system and are suitable for scaleup production...
  27. pmc Comparative analysis of extreme acid survival in Salmonella typhimurium, Shigella flexneri, and Escherichia coli
    J Lin
    Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile 36688, USA
    J Bacteriol 177:4097-104. 1995
    ..The arginine AR system was only observed in E. coli and required stationary-phase induction in acidified complex medium. The product of the adi locus, arginine decarboxylase, was responsible for arginine-based acid survival...
  28. ncbi Different responsiveness of endothelial cells to vascular endothelial growth factor and basic fibroblast growth factor added to culture media under gravity and simulated microgravity
    Daniela Grimm
    Department of Pharmacology, Aarhus University, Bartholin Building, Wilhelm Meyers Allé 4, Building 1240, DK 8000 Aarhus C, Denmark
    Tissue Eng Part A 16:1559-73. 2010
    ..These findings provide evidence that phosphokinase Calpha plays a key role in tube formation. Improving the interaction of VEGF and/or bFGF with EC under s-microg could enhance the engineering of vascular intimas...
  29. ncbi Isolating "uncultivable" microorganisms in pure culture in a simulated natural environment
    T Kaeberlein
    Biology Department, Northeastern University, Boston, MA 02115, USA
    Science 296:1127-9. 2002
    ..These isolates did not grow on artificial media alone but formed colonies in the presence of other microorganisms. This observation may help explain the nature of microbial uncultivability...
  30. pmc Nutritional homeostasis in batch and steady-state culture of yeast
    Alok J Saldanha
    Department of Genetics, Stanford University Medical School, Stanford, CA 94305, USA
    Mol Biol Cell 15:4089-104. 2004
    ....
  31. pmc Induction and resuscitation of viable but nonculturable Salmonella enterica serovar typhimurium DT104
    A R Gupte
    Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 20742, USA
    Appl Environ Microbiol 69:6669-75. 2003
    ..enterica serovar Typhimurium DT104 may be able to respond uniquely to various adverse environmental conditions...
  32. ncbi Comparison of BACTEC 460 and MGIT 960 systems for the culture of Mycobacterium avium subsp. paratuberculosis S strain and observations on the effect of inclusion of ampicillin in culture media to reduce contamination
    S Gumber
    The University of Sydney, Private Bag 3, Camden 2570, NSW, Australia
    Vet Microbiol 119:42-52. 2007
    ..The radiometric BACTEC culture system remains the best alternative for the culture of S strain and is recommended in circumstances where the genotype (s) of the strains present in a region/farm is either unknown or S strain...
  33. pmc Dispersion and stability optimization of TiO2 nanoparticles in cell culture media
    Zhaoxia Ji
    California NanoSystems Institute, Department of Civil and Environmental Engineering, University of California at Los Angeles, Los Angeles, California 90095, USA
    Environ Sci Technol 44:7309-14. 2010
    ..interaction on TiO(2) nanoparticle dispersion in six different mammalian, bacteria, and yeast cell culture media. Great improvement in TiO(2) dispersion was observed upon the addition of BSA, even though the degree of ..
  34. ncbi Bacterial biofilm formation under microgravity conditions
    R J McLean
    Department of Biology, Southwest Texas State University, San Marcos, 78666, USA
    FEMS Microbiol Lett 195:115-9. 2001
    ..These results represent the first report of biofilm formation under microgravity conditions...
  35. ncbi Circumventing the Crabtree effect: replacing media glucose with galactose increases susceptibility of HepG2 cells to mitochondrial toxicants
    Lisa D Marroquin
    Pfizer DSRD, San Diego, CA 92121, USA
    Toxicol Sci 97:539-47. 2007
    ..Some drugs were equally toxic to both glucose- and galactose-grown cells, suggesting that mitochondrial impairment is likely secondary to other cytotoxic mechanisms...
  36. pmc Composition and acidification of the culture medium influences chronological aging similarly in vineyard and laboratory yeast
    Christopher J Murakami
    Department of Pathology, University of Washington, Seattle, Washington, United States of America
    PLoS ONE 6:e24530. 2011
    ..These data support the idea that mechanisms of chronological aging are similar in both the laboratory and vineyard strains...
  37. pmc The fungal pathogen Candida albicans autoinduces hyphal morphogenesis by raising extracellular pH
    Slavena Vylkova
    Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center, Houston, Texas, USA
    MBio 2:e00055-11. 2011
    ..We propose that nutrient-deprived C. albicans cells catabolize amino acids as a carbon source, excreting the amino nitrogen as ammonia to raise environmental pH and stimulate morphogenesis, thus directly contributing to pathogenesis...
  38. ncbi Feeder-independent culture of human embryonic stem cells
    Tenneille E Ludwig
    Genome Center of Wisconsin, University of Wisconsin Madison, 425 Henry Mall, Madison, Wisconsin 53706, USA
    Nat Methods 3:637-46. 2006
  39. ncbi Gene expression profiling of Escherichia coli growth transitions: an expanded stringent response model
    Dong Eun Chang
    Advanced Center for Genome Technology, The University of Oklahoma, Norman, OK 73019 0245, USA
    Mol Microbiol 45:289-306. 2002
    ....
  40. ncbi Derivation of human embryonic stem cells in defined conditions
    Tenneille E Ludwig
    WiCell Research Institute, PO Box 7365, Madison, Wisconsin 53707 7365, USA
    Nat Biotechnol 24:185-7. 2006
    ..We describe the derivation of two new human ES cell lines in these defined culture conditions...
  41. pmc Microscopic observation drug susceptibility assay, a rapid, reliable diagnostic test for multidrug-resistant tuberculosis suitable for use in resource-poor settings
    David A J Moore
    Laboratorio de Investigación de Enfermedades Infecciosas, Universidad Peruana Cayetano Heredia, San Martin de Porras, Lima, Peru
    J Clin Microbiol 42:4432-7. 2004
    ..A comprehensive prospective evaluation of MODS is under way in Perú, and independent validation in nonresearch laboratories should be undertaken at the earliest opportunity...
  42. pmc The micro-Petri dish, a million-well growth chip for the culture and high-throughput screening of microorganisms
    Colin J Ingham
    Top Institute Food and Nutrition, 6703 CT, Wageningen, The Netherlands
    Proc Natl Acad Sci U S A 104:18217-22. 2007
    ....
  43. ncbi Effect of fermentation temperature and culture media on the yeast lipid composition and wine volatile compounds
    Gemma Beltran
    Unitat d Enologia del Centre de Referència de Tecnologia d Aliments, Dept Bioquímica i Biotecnologia, Facultat d Enologia de Tarragona, Universitat Rovira i Virgili, C Marcel li Domingo, s n 43007 Tarragona, Spain
    Int J Food Microbiol 121:169-77. 2008
    ..The aim of this study is to analyse the effect of fermentation temperature (13 degrees C and 25 degrees C) and culture media (synthetic media and grape must) on yeast lipid composition and volatile compounds in wine...
  44. pmc Transcriptional and enzymatic profiling of Pleurotus ostreatus laccase genes in submerged and solid-state fermentation cultures
    Raúl Castanera
    Department of Agrarian Production, Public University of Navarre, Pamplona, Spain
    Appl Environ Microbiol 78:4037-45. 2012
    ..These differences can be targeted for biotechnological breeding programs for enzyme production in submerged fermentation reactors...
  45. ncbi Reprogramming of human fibroblasts to induced pluripotent stem cells under xeno-free conditions
    Ignasi Rodríguez-Pizà
    Center for Regenerative Medicine in Barcelona, Dr Aiguader 88, 08003 Barcelona, Spain
    Stem Cells 28:36-44. 2010
    ..We first developed a xeno-free cell culture media that supported the long-term propagation of human embryonic stem cells (hESCs) to a similar extent as ..
  46. ncbi Embryo culture medium: which is the best?
    Michelle Lane
    Research Centre for Reproductive Health, Discipline of Paediatrics and Reproductive Health, University of Adelaide, Adelaide, South Australia, Australia
    Best Pract Res Clin Obstet Gynaecol 21:83-100. 2007
    ..Therefore, when examining which culture media is the best, it is prudent to not only examine the ability of a culture system to produce a pregnancy with the ..
  47. pmc Bicarbonate ion stimulates the expression of locus of enterocyte effacement-encoded genes in enterohemorrhagic Escherichia coli O157:H7
    Hiroyuki Abe
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    Infect Immun 70:3500-9. 2002
    ....
  48. pmc Time-lapse evaluation of human embryo development in single versus sequential culture media--a sibling oocyte study
    Haydar Nadir Ciray
    Fulya IVF Clinic, Bahceci Health Group, Hakki Yeten cd 11, Terrace Fulya, Istanbul, Turkey
    J Assist Reprod Genet 29:891-900. 2012
    ..To compare the dynamics of early development between embryos cultured in single and sequential media...
  49. pmc Biofilm formation and sloughing in Serratia marcescens are controlled by quorum sensing and nutrient cues
    S A Rice
    The Centre for Marine Biofouling and Bio Innovation, The University of New South Wales, Sydney, NSW 2052 Australia
    J Bacteriol 187:3477-85. 2005
    ..Together, the data suggest that biofilm formation by S. marcescens is a dynamic process that is controlled by both nutrient cues and the quorum-sensing system...
  50. ncbi BMP15 gene is activated during human amniotic fluid stem cell differentiation into oocyte-like cells
    Xiang Cheng
    Department of Animal Biotechnology, College of Veterinary Medicine, Northwest A and F University, Yangling, Shaanxi, China
    DNA Cell Biol 31:1198-204. 2012
    ..Together, these results illustrated that the BMP15 could be used as an oogenesis marker to track hAFSCs differentiation into the OLCs...
  51. ncbi Insulin increases epiblast cell number of in vitro cultured mouse embryos via the PI3K/GSK3/p53 pathway
    Jared M Campbell
    Centre for Stem Cell Research, School of Paediatrics and Reproductive Health, University of Adelaide, Adelaide, South Australia
    Stem Cells Dev 21:2430-41. 2012
    ..Furthermore, we suggest that the inclusion of insulin in culture media could be used as a strategy for increasing the efficiency with which the ESC lines can be derived from ..
  52. ncbi Effect of copper, nutrient nitrogen, and wood-supplement on the production of lignin-modifying enzymes by the white-rot fungus Phlebia radiata
    Miia R Mäkelä
    Department of Food and Environmental Sciences, Division of Microbiology, Viikki Biocenter 1, FIN 00014 Helsinki, Finland
    Fungal Biol 117:62-70. 2013
    ..radiata with pI value of 4.9, thereby complementing our previous findings on gene expression, and cloning of a second laccase of this fungus...
  53. pmc Individual variation in growth factor concentrations in platelet-rich plasma and its influence on human mesenchymal stem cells
    Hee Soon Cho
    Department of Laboratory Medicine, College of Medicine, Yeungnam University, Daegu, Korea
    Korean J Lab Med 31:212-8. 2011
    ..The objective of this study was to explore whether individual variations in the concentration of growth factors (GFs) influence the biologic effects of platelet-rich plasma (PRP) on human mesenchymal stem cells (HMSCs)...
  54. pmc Comparison of different culture media and storage temperatures for the long-term preservation of Streptococcus pneumoniae in the tropics
    G Siberry
    Department of Paediatrics, Johns Hopkins Medical Institutions, 600 N Wolfe Street, Baltimore, MD 21287, USA
    Bull World Health Organ 79:43-7. 2001
    ..We evaluated alternative low-cost methods, by comparing different culture media and storage temperatures.
  55. ncbi Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media
    Caroline Rauch
    Division of Physiology, Innsbruck Medical University, Austria
    ALTEX 28:305-16. 2011
    The search for alternatives to the use of fetal bovine serum (FBS) in cell and tissue culture media has become a major goal in terms of the 3R principles in order to reduce or to avoid harvesting of FBS from bovine fetuses, and, in terms ..
  56. ncbi Regulation of longevity and stress resistance by Sch9 in yeast
    P Fabrizio
    Division of Biogerontology, Andrus Gerontology Center, and Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089 0191, USA
    Science 292:288-90. 2001
    ..These results indicate that longevity is associated with increased investment in maintenance and show that highly conserved genes play similar roles in life-span regulation in S. cerevisiae and higher eukaryotes...
  57. pmc Standardized assay medium to measure Lactococcus lactis enzyme activities while mimicking intracellular conditions
    Anisha Goel
    Centre for Integrative Bioinformatics, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands
    Appl Environ Microbiol 78:134-43. 2012
    ..Yet, the ratios of V(max) over measured in vivo fluxes were above 1. With this work, we have developed and extensively validated standard protocols for enzyme activity measurements for L. lactis...
  58. pmc Degradation of nuclease-stabilized RNA oligonucleotides in Mycoplasma-contaminated cell culture media
    Frank J Hernandez
    Department of Internal Medicine, Roy J and Lucille A Carver College of Medicine, University of Iowa, 375 Newton Road, Iowa City, IA 52242, USA
    Nucleic Acid Ther 22:58-68. 2012
    ..RNA zymogram analysis of conditioned culture media and material centrifuged from the media revealed several distinct protein bands (ranging from 30 to 68 kDa) ..
  59. pmc Effect of modeled reduced gravity conditions on bacterial morphology and physiology
    Raja Vukanti
    Department of Biological Sciences, Kent State University, PO Box 5190, Kent, OH 44242, USA
    BMC Microbiol 12:4. 2012
    ..The parameters selected have not been studied in E. coli and S. aureus under MRG conditions and provide critical information about bacterial viability and potential for population growth...
  60. pmc Enhanced bacterial protein expression during auto-induction obtained by alteration of lac repressor dosage and medium composition
    Paul G Blommel
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biotechnol Prog 23:585-98. 2007
    ..Upon the basis of the analysis of over 500 independent expression results, combinations of optimized expression media and expression plasmids that gave protein yields of greater than 1000 mug/mL of expression culture were identified...
  61. pmc Overflow metabolism in Escherichia coli during steady-state growth: transcriptional regulation and effect of the redox ratio
    G N Vemuri
    Center for Molecular BioEngineering, Driftmier Engineering, University of Georgia, Athens, GA 30602, USA
    Appl Environ Microbiol 72:3653-61. 2006
    ..The results provide a basis for studying new regulatory mechanisms prevalent at reduced NADH/NAD ratios, as well as for designing more efficient bioprocesses...
  62. ncbi Noninvasive metabolomic profiling of embryo culture media using Raman and near-infrared spectroscopy correlates with reproductive potential of embryos in women undergoing in vitro fertilization
    Emre Seli
    Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine, New Haven, CT 06520 8063, USA
    Fertil Steril 88:1350-7. 2007
    To determine if metabolomic profiling of embryo culture media correlates with reproductive potential of individual embryos.
  63. ncbi Instability of, and generation of hydrogen peroxide by, phenolic compounds in cell culture media
    Lee Hua Long
    Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
    Arch Biochem Biophys 501:162-9. 2010
    ..we show that hydroxytyrosol, delphinidin chloride and rosmarinic acid are unstable in three commonly-used cell culture media (Dulbecco's modified Eagle's medium (DMEM), RPMI 1640 (RPMI) and Minimal Essential Medium Eagle (MEM)) and ..
  64. pmc Galactose enhances oxidative metabolism and reveals mitochondrial dysfunction in human primary muscle cells
    Celine Aguer
    Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ontario, Canada
    PLoS ONE 6:e28536. 2011
    ....
  65. pmc Optimization of variable fluorescence measurements of phytoplankton communities with cyanobacteria
    Stefan G H Simis
    Finnish Environment Institute SYKE, Marine Research Centre, Erik Palménin aukio 1, 00560 Helsinki, Finland
    Photosynth Res 112:13-30. 2012
    ..65-0.7...
  66. ncbi Isolation and characterization of a population of immature dental pulp stem cells expressing OCT-4 and other embryonic stem cell markers
    Irina Kerkis
    Laboratorio de Genetica, Instituto Butantan, Sao Paulo, Brasil
    Cells Tissues Organs 184:105-16. 2006
    ..The relative ease of recovery and the expression profiles of various markers justify further exploration of IDPSC for clinical therapy...
  67. pmc Mitochondrial respiratory thresholds regulate yeast chronological life span and its extension by caloric restriction
    Alejandro Ocampo
    Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, FL 33136, USA
    Cell Metab 16:55-67. 2012
    ....
  68. ncbi Specimens and culture media for the laboratory diagnosis of typhoid fever
    John Wain
    The Wellcome Trust Clinical Research Unit, Centre for Tropical Diseases, Ho Chi Minh City, Vietnam
    J Infect Dev Ctries 2:469-74. 2008
    ..However, current methods are not fully optimised and sourcing culture media and bottles for culture media may be problematic.
  69. ncbi Cell volume regulation in oocytes and early embryos: connecting physiology to successful culture media
    Jay M Baltz
    Ottawa Hospital Research Institute, Department of Obstetrics and Gynecology Division of Reproductive Medicine, University of Ottawa, Ottawa, ON, Canada
    Hum Reprod Update 16:166-76. 2010
    ..These could be alleviated by lowering culture medium osmolarity. Because mammalian cells regulate their volumes by adjusting intracellular osmotic pressure, cell volume regulation could be critical to early embryos...
  70. ncbi Biofilm formation and interactions of bacterial strains found in wastewater treatment systems
    Sofia Andersson
    Department of Environmental Microbiology, Royal Institute of Technology, AlbaNova University Centre, Stockholm, Sweden
    FEMS Microbiol Lett 283:83-90. 2008
    ..Four different culture media were used, wastewater, acetate medium, glucose medium and diluted nutrient broth...
  71. ncbi Yarrowia lipolytica as a potential producer of citric acid from raw glycerol
    S Papanikolaou
    Laboratoire des Sciences du Genie Chimique, Vandoeuvre lès Nancy France
    J Appl Microbiol 92:737-44. 2002
    ..To study the biochemical response of Yarrowia lipolytica LGAM S(7)1 during growth on raw glycerol (the main by-product of bio-diesel production units) in order to produce metabolic products of industrial significance...
  72. ncbi Culture media for the differentiation of mesenchymal stromal cells
    Corina Vater
    Department of Orthopedic Surgery, University Hospital Carl Gustav Carus, Dresden, Germany
    Acta Biomater 7:463-77. 2011
    ..Hence, we here summarize the culture media and their compositions currently available for the differentiation of MSCs towards osteogenic, chondrogenic, ..
  73. ncbi How Pseudomonas aeruginosa adapts to various environments: a metabolomic approach
    Eliane Frimmersdorf
    Department of Biochemistry and Bioinformatics, Institute for Biochemistry and Biotechnology, Technische Universitaet Braunschweig, 38106 Braunschweig, Germany
    Environ Microbiol 12:1734-47. 2010
    ..This specified adaptation strategy combined with the invariant basic core metabolism may represent a fundamental requirement for the metabolic versatility of this organism...
  74. pmc High-frequency triazole resistance found In nonculturable Aspergillus fumigatus from lungs of patients with chronic fungal disease
    David W Denning
    National Aspergillosis Centre, University of Manchester, Manchester, UK
    Clin Infect Dis 52:1123-9. 2011
    ..Triazole resistance rates are rising internationally. Microbiological diagnosis of aspergillosis is limited by poor culture yield, leading to uncertainty about the frequency of triazole resistance...
  75. ncbi Heterotrophic growth and lipid production of Chlorella protothecoides on glycerol
    John O'Grady
    School of Chemical Engineering, Purdue University, FRNY Hall, West Lafayette, IN 47907 2050, USA
    Bioprocess Biosyst Eng 34:121-5. 2011
    ..Therefore, we have demonstrated the promise for simultaneous high growth rates and lipid yields of C. protothecoides heterotrophically grown on mixtures of glycerol...
  76. ncbi Polyhydroxyalkanoates: bioplastics with a green agenda
    Tajalli Keshavarz
    Applied Biotechnology Research Group, School of Life Sciences, University of Westminster, United Kingdom
    Curr Opin Microbiol 13:321-6. 2010
    ..The future trend is to focus on the development of more efficient and economical processes for PHA production, isolation, purification and improvement of PHA material properties...
  77. pmc ArgR-regulated genes are derepressed in the Legionella-containing vacuole
    Galadriel Hovel-Miner
    Department of Microbiology and Immunology, Columbia University Medical Center, New York, New York 10032, USA
    J Bacteriol 192:4504-16. 2010
    ..These results suggest that L-arginine availability functions as a regulatory signal during Legionella intracellular growth...
  78. pmc Reduced folate supply as a key to enhanced L-serine production by Corynebacterium glutamicum
    Michael Stolz
    Institute of Biotechnology, Research Centre Juelich, D 52425 Juelich, Germany
    Appl Environ Microbiol 73:750-5. 2007
    ..At 0.1 mM folate, 81 mM L-serine accumulated. In a 20-liter controlled fed-batch culture, a 345 mM L-serine accumulation was achieved. Thus, an efficient and highly competitive process for microbial l-serine production is available...
  79. pmc A novel serum-free medium for the expansion of human mesenchymal stem cells
    Lucas G Chase
    Primary and Stem Cell Systems, Life Technologies, 501 Charmany Drive, Madison, WI 53719, USA
    Stem Cell Res Ther 1:8. 2010
    ..However, despite the remarkable clinical advancements in this field, most applications still use traditional culture media containing fetal bovine serum...
  80. pmc Best practices in heterotrophic high-cell-density microalgal processes: achievements, potential and possible limitations
    Fabian Bumbak
    Institute of Biotechnology, Zurich University of Applied Sciences, Wädenswil, Switzerland
    Appl Microbiol Biotechnol 91:31-46. 2011
    ..The review confirms that, although fundamental knowledge is now available, the development of efficient, economically feasible large-scale bioprocesses remains an obstacle to the commercialisation of this promising technology...
  81. pmc Depletion of pre-16S rRNA in starved Escherichia coli cells
    G A Cangelosi
    Seattle Biomedical Research Institute and Department of Pathobiology, University of Washington 98109, USA
    J Bacteriol 179:4457-63. 1997
    ..This supports the feasibility of using pre-rRNA-targeted probes to monitor bacterial growth in natural systems, with the caveat that patterns of pre-rRNA depletion vary with the conditions limiting growth...
  82. ncbi Effects of gender-specific adult bovine serum on myogenic satellite cell proliferation, differentiation and lipid accumulation
    Dong Mok Lee
    School of Biotechnology, Yeungnam University, Gyeongsan, South Korea
    In Vitro Cell Dev Biol Anim 47:438-44. 2011
    ..Concluding the above facts, the results indicate that each gender-specific bovine serum constitutes of different component, which leads to unique effects on cell behavior...
  83. ncbi End-product induced metabolic shifts in Clostridium thermocellum ATCC 27405
    Thomas Rydzak
    Deparment of Microbiology, University of Manitoba, Winnipeg, MB, Canada
    Appl Microbiol Biotechnol 92:199-209. 2011
    ..We demonstrate that bacterial metabolism may be manipulated in order to selectively improve desired product yields...
  84. ncbi Improved efficiency and pace of generating induced pluripotent stem cells from human adult and fetal fibroblasts
    Prashant Mali
    Stem Cell Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Broadway Research Building, Room 747, 733 North Broadway, Baltimore, Maryland 21205, USA
    Stem Cells 26:1998-2005. 2008
    ..Using this improved approach, we also generated hES-like cells from homozygous fibroblasts containing the sickle cell anemia mutation Hemoglobin Sickle. Disclosure of potential conflicts of interest is found at the end of this article...
  85. ncbi Engineering of pentose transport in Corynebacterium glutamicum to improve simultaneous utilization of mixed sugars
    Miho Sasaki
    Research Institute of Innovative Technology for the Earth, 9 2, Kizugawadai, Kizugawa, Kyoto, 619 0292, Japan
    Appl Microbiol Biotechnol 85:105-15. 2009
    ..In mineral medium containing a sugar mixture of D-glucose, D-xylose, L-arabinose, and D-cellobiose under oxygen-deprived conditions, strain ACX-araE simultaneously and completely consumed all sugars...
  86. ncbi Phosphorylation of the transcription factor PHO4 by a cyclin-CDK complex, PHO80-PHO85
    A Kaffman
    School of Medicine, Department of Biochemistry and Biophysics, University of California at San Francisco 94143
    Science 263:1153-6. 1994
    ..These results demonstrate the existence of a cyclin-cdk complex that is used for a regulatory process other than cell-cycle control and identify a physiologically relevant substrate for this complex...
  87. ncbi Production of arabitol from glycerol: strain screening and study of factors affecting production yield
    Srujana Koganti
    Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH 44325 3906, USA
    Appl Microbiol Biotechnol 90:257-67. 2011
    ..Results from this work show that arabitol is a promising value-added product from glycerol using D. hansenii SBP-1 as the producing strain...
  88. ncbi Effect of nitrogen and phosphorus on growth of toxic and nontoxic Microcystis strains and on intracellular microcystin concentrations
    C Vezie
    Department of Applied Chemistry and Microbiology, Division of Microbiology, Biocenter Viikki, FIN 00014 Helsinki University, Finland
    Microb Ecol 43:443-54. 2002
    ..High levels of nitrogen and phosphorus in freshwaters may favor the growth of toxic Microcystis strains over nontoxic ones...
  89. pmc Shiga toxin-producing Escherichia coli in children: diagnosis and clinical manifestations of O157:H7 and non-O157:H7 infection
    Christina R Hermos
    Division of Infectious Diseases, Children s Hospital Boston, Boston, Massachusetts, USA
    J Clin Microbiol 49:955-9. 2011
    ..The Premier EHEC assay was significantly more sensitive than SMAC culture for diagnosis of STEC, and O157:H7 and non-O157:H7 STEC caused infections of similar severity in children...
  90. pmc Quantitative analysis of population heterogeneity of the adaptive salt stress response and growth capacity of Bacillus cereus ATCC 14579
    Heidy M W den Besten
    TI Food and Nutrition, Wageningen, The Netherlands
    Appl Environ Microbiol 73:4797-804. 2007
    ....
  91. pmc Isolation of a mesenchymal cell population from murine dermis that contains progenitors of multiple cell lineages
    Lauren Crigler
    Laboratory of Cellular Carcinogenesis and Tumor Promotion, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland, USA
    FASEB J 21:2050-63. 2007
    ..Moreover, these cells could be available in sufficient quantities for lineage determination or tissue engineering applications...
  92. ncbi Medium optimization for hen egg white lysozyme production by recombinant Aspergillus niger using statistical methods
    R Gheshlaghi
    Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, N2L 3G1 Waterloo, Ontario, Canada
    Biotechnol Bioeng 90:754-60. 2005
    ..5 g L-1, and CaCl2.2H2O 0.5 g L-1. This medium was projected to produce, theoretically, 212 mg L-1 lysozyme. Using this medium, an experimental maximum lysozyme concentration of 209+/-18 mg L-1 verified the applied methodology...
  93. ncbi Purification and germination of Candida albicans and Candida dubliniensis chlamydospores cultured in liquid media
    Francesco Citiulo
    Microbiology Research Unit, Division of Oral Biosciences, Dublin Dental School and Hospital, Trinity College Dublin, University of Dublin, Dublin 2, Ireland
    FEMS Yeast Res 9:1051-60. 2009
    ..Instead, our data suggest that chlamydospores represent an alternative specialized form of growth by C. albicans and C. dubliniensis...
  94. pmc Effects of growth medium, inoculum size, and incubation time on culturability and isolation of soil bacteria
    Kathryn E R Davis
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia
    Appl Environ Microbiol 71:826-34. 2005
    ....
  95. ncbi Detection of volatile metabolites produced by bacterial growth in blood culture media by selected ion flow tube mass spectrometry (SIFT-MS)
    Randall A Allardyce
    Department of Surgery, Christchurch School of Medicine and Health Sciences, Christchurch, New Zealand
    J Microbiol Methods 65:361-5. 2006
    ..Pseudomonas aeruginosa, Streptococcus pneumoniae, Escherichia coli, Staphylococcus aureus and Neisseria meningitidis growth and trace gas patterns were detected from 10 colony forming units after 6 h...
  96. ncbi Pyruvate and oxygen consumption throughout the growth and development of murine oocytes
    Sarah E Harris
    Reproduction and Early Development Research Group, Division of Epidemiology and Biostatistics, Leeds Institute of Genetics Health and Therapeutics, School of Medicine, University of Leeds, Leeds, UK
    Mol Reprod Dev 76:231-8. 2009
    ..To conclude, pyruvate and oxygen were consumed throughout oocyte development at increasing rates. When oocyte cellular volume was accounted for, oocytes from primary follicles displayed greatest metabolic rates...
  97. ncbi Aged mother cells of Saccharomyces cerevisiae show markers of oxidative stress and apoptosis
    P Laun
    Department of Genetics, University of Salzburg, Hellbrunnerstr. 34, A-5020 Salzburg, Austria
    Mol Microbiol 39:1166-73. 2001
    ....
  98. ncbi Why do we still use serum in the production of biopharmaceuticals?
    G Shah
    Glaxo Wellcome R and D, Stevenage, Hertfordshire, UK
    Dev Biol Stand 99:17-22. 1999
    ..Regulatory constraints generally make it impractical and uneconomic to alter existing biopharmaceutical production processes in which serum is used as a raw material...
  99. ncbi Domestication of wild Saccharomyces cerevisiae is accompanied by changes in gene expression and colony morphology
    Martin Kuthan
    Department of Genetics and Microbiology, Charles University, Vinicna 5, 12844 Prague 2, Czech Republic
    Mol Microbiol 47:745-54. 2003
    ..The growth in fluffy colonies may represent a metabolic strategy for survival of yeast under unfavourable conditions that is switched off under felicitous laboratory conditions...
  100. ncbi Hierarchical and metabolic regulation of glucose influx in starved Saccharomyces cerevisiae
    Sergio Rossell
    Department of Molecular Cell Physiology, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, De Boelelaan 1085, NL 1081 HV Amsterdam, The Netherlands
    FEMS Yeast Res 5:611-9. 2005
    ..The method is expounded in terms of its implications for diverse types of regulation. It is also fine-tuned for cases where isoenzymes catalyze the flux through a single metabolic step...
  101. pmc Individual Mycobacterium tuberculosis resuscitation-promoting factor homologues are dispensable for growth in vitro and in vivo
    JoAnn M Tufariello
    Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Infect Immun 72:515-26. 2004
    ..tuberculosis rpf genes, while overlapping to various degrees, are not uniform. We also provide evidence that mycobacterial rpf genes are expressed in vivo in the lungs of mice acutely infected with virulent M. tuberculosis...

Research Grants64

  1. NIH Director's Pioneer Award
    Junying Yuan; Fiscal Year: 2009
    ..No Abstract provided ..
  2. Cross-talk Between Coagulation, Inflammation, and Immunity
    Lawrence L Leung; Fiscal Year: 2013
    ..We will characterize chemerin signaling of U87 cells and test whether chemerin or post-culture media from GBM cells will recruit plasmacytoid dendritic cells...
  3. Novel DNA-Launched Attenuated Vaccine for VEE Virus
    PETER M PUSHKO; Fiscal Year: 2012
    ..The i-DNA clones will be transfected in CHO cells and live attenuated viruses will be harvested from culture media. Safety of i-DNA-derived viruses will be evaluated in mice in collaboration with the Institute of Human ..
  4. Plasma RNA based Early Lung Cancer Detection by Tethered Cationic Lipoplex Assay
    SERGE P NANA-SINKAM; Fiscal Year: 2013
    ..This tCLN biochip has been successfully demonstrated in comparing exosomes isolated from cell culture media of a lung cancer cell line, A549 and a normal epithelial cell line using miR-21 locked nucleic acid (LNA) MB ..
  5. Changes in antioxidants and susceptibility to parasites
    DEBORAH KRISTAN; Fiscal Year: 2012
    ..will be measured using ELISA and mature worms will be removed from mice, measured and then placed in culture media to determine worm survival and egg output...
  6. Functional characterization of hsa-mir-939 as a novel regulator of pain
    Seena Ajit; Fiscal Year: 2013
    ..implication of the presence of circulating miRNAs, we will characterize the exosomes collected from cell culture media and blood...
  7. Induced Reactive Oxygen Species in a 3-D Human Pancreatitis Model in Vitro
    Gregory P Botta; Fiscal Year: 2013
    ..composed of nestin-positive, immortalized, pancreatic epithelial cells, Matrigel scaffolds, predetermined culture media, and specific growth factors...
  8. Biomechanics and Inflammation in Osteoarthritis
    Farshid Guilak; Fiscal Year: 2012
    ..of cartilage metabolism and inflammation in tissues and in fluids such as synovial fluid, serum, and tissue culture media. This strategy allows a mechanistic investigation of the overall theme of the project in a manner that can ..
  9. Delivery of passive immunity against HIV using commensal vaginal Lactobacillus bi
    Laurel A Lagenaur; Fiscal Year: 2012
    ..The secreted antibodies in culture media will be purified using affinity chromatography and tested for neutralization of primary HIV-1 isolates in cell ..
  10. Oxidative protein folding of pilins and virulence in Gram-positive bacteria
    MELISSA ELIZABETH ROBINSON; Fiscal Year: 2013
    ..When cysteine residues forming these were mutated, FimA and SpaA were no longer polymerized, but secreted into culture media as monomers and degradation products...
  11. A Novel Method to Improve Proliferation and Neural Induction of Human MSCs
    Bradley H Garcia; Fiscal Year: 2012
    ..The technology consists of proprietary cell culture media additives that effectively recreate native biophysical properties of the cellular microenvironment and ..
  12. Magnetophoretic Cell Sorting and Analysis
    Maciej Zborowski; Fiscal Year: 2013
    ..increase in the cell magnetophoretic mobility following the addition of soluble iron compounds to the culture media, suggesting an effect of intracellular iron uptake on the cell magnetization...
  13. Mechanism of Neurotrophin Latency Response in a HSV1 Vestibular Neuritis Model
    PAMELA CAROL ROEHM; Fiscal Year: 2013
    ..nerve growth factor (NGF) is necessary for maintenance of the latent state, and withdrawal of NGF from cell culture media results in loss of latency and a lytic infection...
  14. High throughput cell migration assay amenable to high content imaging
    TIMOTHY GEORGE BURLAND; Fiscal Year: 2012
    ..collagen I coated surfaces, b) had the ability to block cell attachment while completely dissolving in tissue culture media, c) permitted cell migration upon dissolution and d) did not interfere with cell viability or induce ..
  15. Inhibiting protein-primed replication of human enteroviruses.
    Catherine H Schein; Fiscal Year: 2013
    ..assays according to their pharmaceutical properties (low toxicity, good solubility and high stability in cell culture media). Compounds may be formulated in nanoparticles to enhance cell uptake...
  16. Mechanisms of Reduced Fecundity in Endometriosis: A role for MMPs and TIMPs
    KATHY L TIMMS; Fiscal Year: 2012
    ..exciting preliminary data shows Sham rat embryos develop endometriosis-like anomalies by addition of TIMP-1 to culture media or by intra-abdominal injection of TIMP-1...
  17. REGULATION OF CHOLESTEROL METABOLISM
    Shunlin Ren; Fiscal Year: 2010
    ..and determine the chemical structures of the novel sulfated oxysterol(s) in nuclei, mitochondria, and culture media;to chemically synthesize this nuclear sulfated oxysterol;2...
  18. Understanding liver bud emergence, formation and potential
    Kimberly D Tremblay; Fiscal Year: 2013
    ..downregulate candidate pathways in localized endodermal domains and the addition of inhibitors directly to the culture media. The manipulated embryos are then cultured through the liver bud phase of development...
  19. Mycobacterium avium subspecies in Crohn's Disease
    Saleh Naser; Fiscal Year: 2005
    ..specimens directly by using nested PCR, RT-PCR and FISH and indirectly by culture using a newly developed culture media appropriate for isolation of cell wall deficient form of MAP...
  20. SOLUBLE ISOFORMS OF HLA-G: STRUCTURE, REGULATION AND FUNCTION
    JOAN SHERAR HUNT; Fiscal Year: 2011
    ..documents associations between pregnancy success and high levels of soluble HLA-G in the supernatant culture media of in vitro cultured embryos, and critical preliminary experiments in our laboratory indicate that women who ..
  21. Noninvasive assessment of embryo implantation potential using 1H NMR metabonomics
    Paolo Rinaudo; Fiscal Year: 2010
    ..magnetic resonance spectroscopy (1H-NMR) show that multivariate analysis of the NMR spectra recorded for culture media samples from 100% and 0% implantation cases revealed a statistical separation of the two groups (p=0.009)...
  22. Directed Culturing of Pneumocystis Using Metatranscriptomics
    Alexey Porollo; Fiscal Year: 2013
    ..ligands, substrates and products, to select candidate supplements for testing in vitro;3) Evaluate modified culture media in a short term in vitro culture assay using a high throughput iterative strategy to identify essential ..
  23. Nanoparticle Effects on Epithelial Cell Protein Expression and Function
    Frank A Witzmann; Fiscal Year: 2012
    ..In addition, the studies will correlate the changes in protein expression with changes in cellular function at both toxic and subtoxic levels. ..
  24. A Novel Method to Improve Function of SC-Derived Hepatocytes
    SCOTT ALLEN MONSMA; Fiscal Year: 2012
    ..The new technology uses proprietary cell culture media additives to recreate native biophysical properties of the cellular microenvironment and encourage cells to ..
  25. Testing of novel system to deliver VRC01 in mouse model
    Hans Peter Kiem; Fiscal Year: 2012
    ..Our first specific aim will define vector and culture media requirements necessary to most efficiently transduce and expand modified immune cells...
  26. Thiol-Ene Click Hydrogels for in situ Cell Expansion and Differentiation
    Chien Chi Lin; Fiscal Year: 2012
    ..The differentiation process will be a combinatorial result of using serum-free culture media, affinity-based recruitment of basic fibroblast growth factor (bFGF), and timely conjugation of glucagon-like ..
  27. ENGINEERING FORM AND FUNCTION IN NEURONAL NETWORKS
    Bruce C Wheeler; Fiscal Year: 2013
    ..The "brain on a chip" technology employed combines precisely controlled cell culture, state-of-the-art cell culture media, large scale microelectrode arrays, newly developed directional microtunnels for guiding neural growth with ..
  28. The chemical biology of pharmacological ascorbate in cancer treatment
    Garry R Buettner; Fiscal Year: 2013
    ..The rationale for this hypothesis is that: (1) AscH- readily autoxidize producing a flux of H2O2 (in cell culture media and in vivo);(2) the high levels of extra cellular AscH- achieved by i.v...
  29. Induction of Vascular Morphogenesis
    Luisa Iruela-Arispe; Fiscal Year: 2013
    ..Our data revealed that mechanical forces from shear fluid (namely, culture media) flow are critical to the development of stable endothelial networks that can, upon transplantation, connect ..
  30. Regulation of Growth and Differentiation in 3-D Cultures of Olfactory Epithelium
    James E Schwob; Fiscal Year: 2012
    ..In this case, culture media conditioned by the growth of a lamina propria cell line (derived from the part of the mucosa that is deep to ..
  31. THROMBOPOIETIN: IMMUNOASSAY AND CHARACTERIZATION
    TED MC DONALD; Fiscal Year: 1993
    ..utilizing a variety of physical chemical and immunological methods: 1) sera from thrombocytopenic animals and culture media from HEK cell cultures will be utilized for TSF production; 2) antisera will be raised in animals by use of ..
  32. Method for Detection of Secreted Proteins in Single Cell Assays
    Henryk Szmacinski; Fiscal Year: 2012
    ..Developing a procedure for reproducible fabrication of substrates with metallic nanostructures that perform in culture media for a prolonged length of time and result in the desired sensitivity for cytokine detection...
  33. PET and MR-Compatible Bioreactor for Cross-Platform Biomarker Development
    Henry F VanBrocklin; Fiscal Year: 2013
    ..the monitoring of metabolic fluxes with high temporal resolution (seconds) without background signals from the culture media. The addition of positron emission tomography (PET) imaging with an array of labeled tracers provides yet ..
  34. A Novel High Throughput Tumor Spheroid Microtechnology
    Hossein Tavana; Fiscal Year: 2013
    ..Importantly the overlay of culture media provides nutrients and minimizes the well-known problem of evaporation and changes in osmolality of media as ..
  35. OXIDATIVE DAMAGE AND IRON REQUIREMENTS OF THE BLASTOCYST
    COLE MANES; Fiscal Year: 1991
    ..The work will require exposing rabbit morulae to various culture media during the initial 48 hours of expansion...
  36. Chemical and Biological Studies of the Guadinomines
    AARON ELIJAH MAY; Fiscal Year: 2013
    ..Natural guadinomines A and B are present at levels of ~1 mg/L of culture media, and require tedious purification procedures...
  37. IGF::OT::IGF DEVELOPMENT OF A VATPASE INHIBITOR AS A RADIOSENSITIZER
    Donald Stewart; Fiscal Year: 2013
    ..Development of the inhibitor begins with its preparation and determination of its lifetime in cell culture media and in preparation of stable, concentrated dosing solutions suitable for continuous infusion...
  38. Regulation of Cranial Suture Development by TGF-Beta and Fibroblast Growth Factor
    Arun Gosain; Fiscal Year: 2007
    ..Additionally, protein levels of TGF- beta1, FDF-2, and noggin will be measured in culture media in vitro using ELISA...
  39. Supporting Human ART Through Basic Science
    Barry Bavister; Fiscal Year: 2004
    ..success rates of bovine offspring production from in vitro matured oocytes, development of specialized embryo culture media, and basic understanding of regulatory mechanisms and anomalies in animal IVP embryos...
  40. ROLE OF PHOSPHOLIPIDS IN MINERALIZATION OF OSTEOID
    David Howell; Fiscal Year: 1992
    ..characterization will be based on the results of endothelial cell chemotaxis assays of extracted tissues and culture media after heparin- Sepharose chromatography, Western blots of isolated growth factors and immunolocalization with ..
  41. Recombinant human serum albumin for use in cell culture media
    Ning Huang; Fiscal Year: 2007
    ..by applicant): Plasma-derived human serum albumin (pHSA) or bovine serum albumin are commonly added to cell culture media used in cell culture-based vaccine and therapeutic production, because it enhances cell growth and increases ..
  42. VITAMIN B-6 AND STERIOD HORMONE ACTION
    JOHN CIDLOWSKI; Fiscal Year: 1991
    ..the past granting period have revealed that physiological concentrations of Vitamin B6 present in most cell culture media suppress or attenuate the responsiveness of cells to glucocorticoids...
  43. CELLULAR MECHANISMS OF CHOLECYSTITIS AND COLITIS
    DONALD KAMINSKI; Fiscal Year: 1999
    ..produce production of inflammatory mediators, however, these inflammatory stimuli and mediators in tissue culture media have no effect on cell integrity...
  44. In vitro culture and transformation of Wolbachia from mosquitoes
    Ann Fallon; Fiscal Year: 2007
    ..We will manipulate folic acid levels in mosquito cell culture media to require the mosquito cell to maintain Wolbachia as the source of folic acid, an essential vitamin...
  45. Enhanced Phototoxicity Assay in Reconstituted Skin
    GEORGE DEGEORGE; Fiscal Year: 2002
    ..formulations can be tested, in contrast to the 3T3 NRU Assay, in which test substances need to dosed via the culture media; 3) the human primary keratinocyte-based tissues are a more relevant model than a mouse tumor cell line...
  46. RETINOID-BINDING PROTEINS
    PER PETERSON; Fiscal Year: 1992
    ..Moreover, stably transfected cell lines manufacturing RBP, grown in normal and vitamin A- deficient culture media, will be employed to unravel why retinol- deficient RBP molecules do not exit the endoplasmic reticulum...
  47. NPY AND ATP COTRANMISSION IN CHROMAFFIN CELLS
    TERRY HEXUM; Fiscal Year: 2003
    ..cell intracellular Ca2+ concentration ([Ca2+]i) or inositol phosphate formation unless ATP is added to culture media along with NPY...
  48. PHOSPHOLIPIDS AND MINERALIZATION OF OSTEOID
    David Howell; Fiscal Year: 1999
    ..characterization will be based on the results of endothelial cell chemotaxis assays of extracted tissues and culture media after heparin- Sepharose chromatography, Western blots of isolated growth factors and immunolocalization with ..
  49. HORMONAL CONTROL OF THE OVIDUCT AND UTERUS
    HAROLD VERHAGE; Fiscal Year: 1990
    ..Secretory activity will be monitored by placing minced endometrial tissue in culture media containing labelled amino acids and sugars and incubated for 24 hours...
  50. SRC KINASE, PATHWAYS MODULATING LH RESPONSIVENESS
    Christopher Taylor; Fiscal Year: 1999
    ..LH-responsiveness will be determined by cAMP and progesterone accumulation in culture media and PDE activity in response to a 24 hour challenge with LH...
  51. SPERM SURVIVAL AND CAPACITATION IN OVIDUCT CELL CULTURES
    JOANNA ELLINGTON; Fiscal Year: 1999
    ..subsequently maintain superior motility, viability and capacitation rates over that seen for sperm in routine culture media. The general hypothesis of this proposal is that human sperm can interact with OEC in vitro and undergo ..
  52. MODULATION OF ORI-BETA REPLICATION ORIGIN BY FISH OIL
    Nawfal Istfan; Fiscal Year: 2004
    ..and the change in chromatin structure are reversed by addition of the polyamine precursor putrescine to the culture media. Our hypothesis is that fish oil affects the duration of S-phase by altering the spatial and/or temporal ..
  53. Creation of an In Vitro Brain Barrier Transport System
    Wei Zheng; Fiscal Year: 2006
    ..of the monolayer of Z310 epithelial cells can be improved by alteration of the chemical components of the culture media, by induction and promotion of tight junction assembly, and/or by genetic modulation of the expression of ..
  54. ARSENIC TRIOXIDE TREATMENT OF LYMPHOPROLIFERATIVE DISORD
    Samuel Waxman; Fiscal Year: 2003
    ..interpreted with caution since cellular levels of glutathione and H202 may be artifactually altered in tissue culture media and are likely to differ from that of cells in vivo...
  55. An in vivo metastasis sensor
    Lonnie D Shea; Fiscal Year: 2013
    ..combination of a cellular array for the high throughput analysis of TF activity, and proteomics analysis of culture media. The cell array will identify the key pathways within metastatic cells responsible fo homing and colonization, ..
  56. FAMILIAL DEFECTIVE APO-B100
    THOMAS INNERARITY; Fiscal Year: 1993
    ..The "LDL" or LDL-like particles will be isolated from cell culture media and from the plasma of transgenic mice, characterized, and tested for LDL receptor binding...
  57. CONTROL OF CELL GROWTH, DIFFERENTIATION, AND FUNCTION
    CHARITY WAYMOUTH; Fiscal Year: 1980
    ..and function of several cell types, including some hormone-dependent epithelial cells, in autoclavable defined culture media incorporating these innovations, will be assembled...
  58. EPIGENETIC CONTROL OF NORMAL EMBRYO DEVELOPMENT IN VITRO
    Barry Bavister; Fiscal Year: 1999
    DESCRIPTION: The long-term goal of this proposal is to devise new culture media that support normal preimplantation embryo development...
  59. Expression of Human Transferrin in Rice Grain
    Deshui Zhang; Fiscal Year: 2009
    Transferrin is an essential ingredient used in cell culture media due to its crucial role in regulating cellular iron uptake, transport, and utilization, and also a promising drug carrier to increase drug therapeutic index via its ..
  60. MECHANISMS OF RHINOVIRUS INDUCED ASTHMA
    William Busse; Fiscal Year: 2002
    ..The resulting culture media will be assessed for cytokine content (ELISA and RT-PCR) and for its ability to activate pulmonary ..
  61. LEISHMANIA DONOVANI SURFACE MEMBRANES: 3 NUCLEOTIDASE
    Michael Gottlieb; Fiscal Year: 1990
    ..and in the insect trypanosomatid Crithidia luciliae is regulated by the supply of available purines in culture media, i.e...
  62. Selenium, Aminothiols, and Radiation
    Alan Diamond; Fiscal Year: 2004
    ..Changes in the expression of stress response genes due to low level selenium supplementation of tissue culture media will also be investigated...
  63. Osteoblast Derived Pathogenic Factors in Hyp Mice
    Norimoto Yanagawa; Fiscal Year: 2005
    ..these bone derived pathogenic factors, we have identified two Hyp-specific proteins from the conditioned culture media derived from in vitro cultured Hyp mouse osteoblast cells (OC)...
  64. ROLE OF ENDOGENOUS LEUKOTRIENE PRODUCTION IN LYMPHOCYTE
    DURGAPRASADARAO ATLURU; Fiscal Year: 1991
    ..studies on arachidonic acid metabolism of T cells included the addition of exogenous arachidonic acid to the culture media, this calls into question in generally accepted concept that T cells do not metabolize arachidonic acid in a ..