bioreactors

Summary

Summary: Tools or devices for generating products using the synthetic or chemical conversion capacity of a biological system. They can be classical fermentors, cell culture perfusion systems, or enzyme bioreactors. For production of proteins or enzymes, recombinant microorganisms such as bacteria, mammalian cells, or insect or plant cells are usually chosen.

Top Publications

  1. ncbi Transgenic chickens as bioreactors for protein-based drugs
    Simon G Lillico
    Roslin Institute, Roslin, Midlothian EH25 9PS, UK
    Drug Discov Today 10:191-6. 2005
  2. pmc Novel approach of high cell density recombinant bioprocess development: optimisation and scale-up from microliter to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures
    Juozas Siurkus
    Laboratory of Bioprocess Engineering, Department of Biotechnology, Technische Universitat Berlin, Ackerstr 71 76, D 13355 Berlin, Germany
    Microb Cell Fact 9:35. 2010
  3. ncbi Challenges in engineering microbes for biofuels production
    Gregory Stephanopoulos
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 315:801-4. 2007
  4. ncbi Physiological responses to mixing in large scale bioreactors
    S O Enfors
    Department of Biotechnology, Royal Institute of Technology, S 10044, Stockholm, Sweden
    J Biotechnol 85:175-85. 2001
  5. ncbi Inhibition of anaerobic digestion process: a review
    Ye Chen
    Department of Biological and Agricultural Engineering, North Carolina State University, Raleigh, NC 27695 7625, USA
    Bioresour Technol 99:4044-64. 2008
  6. ncbi Deciphering the evolution and metabolism of an anammox bacterium from a community genome
    Marc Strous
    Department of Microbiology, IWWR, Radboud University Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands
    Nature 440:790-4. 2006
  7. ncbi Development, parallelization, and automation of a gas-inducing milliliter-scale bioreactor for high-throughput bioprocess design (HTBD)
    R Puskeiler
    Lehrstuhl für Bioverfahrenstechnik, Technische Universitat Munchen, Boltzmannstrasse 15, 85748 Garching, Germany
    Biotechnol Bioeng 89:512-23. 2005
  8. ncbi The role of bioreactors in tissue engineering
    Ivan Martin
    Departments of Surgery and of Research, University Hospital Basel, Hebelstrasse 20, ZLF, Room 405, 4031, Basel, Switzerland
    Trends Biotechnol 22:80-6. 2004
  9. ncbi Biodiesel from microalgae
    Yusuf Chisti
    Institute of Technology and Engineering, Massey University, Private Bag 11 222, Palmerston North, New Zealand
    Biotechnol Adv 25:294-306. 2007
  10. ncbi Microbial fuel cells: novel biotechnology for energy generation
    Korneel Rabaey
    Laboratory of Microbial Ecology and Technology LabMET, Ghent University, Coupure Links 653, B 9000 Ghent, Belgium
    Trends Biotechnol 23:291-8. 2005

Detail Information

Publications339 found, 100 shown here

  1. ncbi Transgenic chickens as bioreactors for protein-based drugs
    Simon G Lillico
    Roslin Institute, Roslin, Midlothian EH25 9PS, UK
    Drug Discov Today 10:191-6. 2005
    ....
  2. pmc Novel approach of high cell density recombinant bioprocess development: optimisation and scale-up from microliter to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures
    Juozas Siurkus
    Laboratory of Bioprocess Engineering, Department of Biotechnology, Technische Universitat Berlin, Ackerstr 71 76, D 13355 Berlin, Germany
    Microb Cell Fact 9:35. 2010
    ..Performing the screening already under fed-batch conditions would limit the amount of work and guarantee that the selected favoured conditions also work in the production scale...
  3. ncbi Challenges in engineering microbes for biofuels production
    Gregory Stephanopoulos
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 315:801-4. 2007
    ..There is justified optimism that the full potential of biofuel production from cellulosic biomass will be obtainable in the next 10 to 15 years...
  4. ncbi Physiological responses to mixing in large scale bioreactors
    S O Enfors
    Department of Biotechnology, Royal Institute of Technology, S 10044, Stockholm, Sweden
    J Biotechnol 85:175-85. 2001
    ..Flow cytometric analysis revealed reduced damage with respect to cytoplasmic membrane potential and integrity in cells grown in the dynamic environments of the large scale reactor and the scale-down reactor...
  5. ncbi Inhibition of anaerobic digestion process: a review
    Ye Chen
    Department of Biological and Agricultural Engineering, North Carolina State University, Raleigh, NC 27695 7625, USA
    Bioresour Technol 99:4044-64. 2008
    ..Co-digestion with other waste, adaptation of microorganisms to inhibitory substances, and incorporation of methods to remove or counteract toxicants before anaerobic digestion can significantly improve the waste treatment efficiency...
  6. ncbi Deciphering the evolution and metabolism of an anammox bacterium from a community genome
    Marc Strous
    Department of Microbiology, IWWR, Radboud University Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands
    Nature 440:790-4. 2006
    ..Most significantly, we identified candidate genes responsible for ladderane biosynthesis and biological hydrazine metabolism, and discovered unexpected metabolic versatility...
  7. ncbi Development, parallelization, and automation of a gas-inducing milliliter-scale bioreactor for high-throughput bioprocess design (HTBD)
    R Puskeiler
    Lehrstuhl für Bioverfahrenstechnik, Technische Universitat Munchen, Boltzmannstrasse 15, 85748 Garching, Germany
    Biotechnol Bioeng 89:512-23. 2005
    ..impeller was developed with oxygen transfer coefficients as high as in laboratory and industrial stirred-tank bioreactors. The bioreactor reaches oxygen transfer coefficients of >0.4 s(-1). Oxygen transfer coefficients of >0...
  8. ncbi The role of bioreactors in tissue engineering
    Ivan Martin
    Departments of Surgery and of Research, University Hospital Basel, Hebelstrasse 20, ZLF, Room 405, 4031, Basel, Switzerland
    Trends Biotechnol 22:80-6. 2004
    ..In addition, by automating and standardizing tissue manufacture in controlled closed systems, bioreactors could reduce production costs, thus facilitating a wider use of engineered tissues.
  9. ncbi Biodiesel from microalgae
    Yusuf Chisti
    Institute of Technology and Engineering, Massey University, Private Bag 11 222, Palmerston North, New Zealand
    Biotechnol Adv 25:294-306. 2007
    ..Oil productivity of many microalgae greatly exceeds the oil productivity of the best producing oil crops. Approaches for making microalgal biodiesel economically competitive with petrodiesel are discussed...
  10. ncbi Microbial fuel cells: novel biotechnology for energy generation
    Korneel Rabaey
    Laboratory of Microbial Ecology and Technology LabMET, Ghent University, Coupure Links 653, B 9000 Ghent, Belgium
    Trends Biotechnol 23:291-8. 2005
    ..Here we discuss how bacteria use an anode as an electron acceptor and to what extent they generate electrical output. The MFC technology is evaluated relative to current alternatives for energy generation...
  11. ncbi Tissue engineering--current challenges and expanding opportunities
    Linda G Griffith
    Department of Chemical Engineering, Division of Bioengineering and Environmental Health, and Biotechnology Process Engineering Center, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 295:1009-14. 2002
    ....
  12. ncbi Large-scale expansion of pluripotent human embryonic stem cells in stirred-suspension bioreactors
    Roman Krawetz
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada
    Tissue Eng Part C Methods 16:573-82. 2010
    ..Suspension bioreactors are an effective alternative to static culture flasks as they enable the generation of clinically relevant cell ..
  13. ncbi Characterization of microbial biofilms in a thermophilic biogas system by high-throughput metagenome sequencing
    Antje Rademacher
    Leibniz Institut für Agrartechnik Potsdam Bornim e V ATB, Abteilung Bioverfahrenstechnik, Potsdam, Germany
    FEMS Microbiol Ecol 79:785-99. 2012
    ..Finally, the metagenome analysis unveiled a large number of reads with unidentified microbial origin, indicating that the anaerobic degradation process may also be conducted by up to now unknown species...
  14. pmc Molecular microbial diversity of an anaerobic digestor as determined by small-subunit rDNA sequence analysis
    J J Godon
    Laboratoire de Biotechnologie de l Environnement, Institut National de la Recherche Agronomique, Narbonne, France
    Appl Environ Microbiol 63:2802-13. 1997
    ..In contrast, the three other Archaea OTUs are unusual and are related to thermophilic microorganisms such as Crenarchaea or Thermoplasma spp. Five percent of the sequences analyzed were chimeras and were removed from the analysis...
  15. ncbi Correlating microbial community structure and composition with aeration intensity in submerged membrane bioreactors by 454 high-throughput pyrosequencing
    Jinxing Ma
    State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, No 1239 of Siping Road, Shanghai 200092, PR China
    Water Res 47:859-69. 2013
    ..high-throughput pyrosequencing was applied to analyze the 16S rRNA gene of bacteria in two submerged membrane bioreactors (MBRs) under low (R(L)) and high aeration (R(H)) conditions...
  16. ncbi Microbial structures, functions, and metabolic pathways in wastewater treatment bioreactors revealed using high-throughput sequencing
    Lin Ye
    Environmental Biotechnology Laboratory, The University of Hong Kong, Pokfulam Road, Hong Kong
    Environ Sci Technol 46:13244-52. 2012
    ..structures, functional profiles, and metabolic pathways in a lab-scale and a full-scale wastewater treatment bioreactors. In order to do this, over 12 gigabases of metagenomic sequence data and 600,000 paired-end sequences of ..
  17. ncbi Plants as bioreactors: Recent developments and emerging opportunities
    Arun K Sharma
    Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi 110021, India
    Biotechnol Adv 27:811-32. 2009
    In recent years, the use of plants as bioreactors has emerged as an exciting area of research and significant advances have created new opportunities...
  18. ncbi Diversity and dynamics of microbial communities in engineered environments and their implications for process stability
    Aurelio Briones
    Department of Civil and Environmental Engineering, University of Illinois at Urbana Champaign, 205 North Mathews Avenue, Urbana, IL 61801, USA
    Curr Opin Biotechnol 14:270-6. 2003
    The availability of molecular biological tools for studying microbial communities in bioreactors and other engineered systems has resulted in remarkable insights linking diversity and dynamics to process stability...
  19. ncbi Analysis of the bacterial community in a laboratory-scale nitrification reactor and a wastewater treatment plant by 454-pyrosequencing
    Lin Ye
    Environmental Biotechnology Laboratory, Genome Research Center, The Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China
    Water Res 45:4390-8. 2011
    For full understanding of the microbial community in the wastewater treatment bioreactors, one of the feasible and effective ways is to investigate the massive genetic information contained in the activated sludge...
  20. ncbi Development of high-rate anaerobic ammonium-oxidizing (anammox) biofilm reactors
    Ikuo Tsushima
    Department of Urban and Environmental Engineering, Graduate School of Engineering, Hokkaido University, Sapporo 060 8628, Japan
    Water Res 41:1623-34. 2007
    ..e., simultaneous consumption of NH(4)(+) and NO(2)(-)) of 4.45 g-N of (NH(4)(+)+NO(2)(-))m(-2)day(-1) was detected in the upper 800 microm of the biofilm, which was consistent with the spatial distribution of anammox bacteria...
  21. ncbi Aerobic granular sludge: recent advances
    Sunil S Adav
    Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan
    Biotechnol Adv 26:411-23. 2008
    ..The review attempts to shed light on the fundamental understanding in aerobic granulation by newly employed confocal laser scanning microscopic techniques and microscopic observations of granules...
  22. ncbi Anaerobic sludge granulation
    L W Hulshoff Pol
    Sub Department of Environmental Technology, Agricultural University of Wageningen, Biotechnion Bomenweg 2, PO Box 8129, 6700 EV Wageningen, The Netherlands
    Water Res 38:1376-89. 2004
    ..Granule enhancing factors in the latter stages predominantly rely on manipulation of the selection pressure, through which selectively heavier sludge particles are retained in the UASB reactor...
  23. ncbi Phylogenetic characterization of a biogas plant microbial community integrating clone library 16S-rDNA sequences and metagenome sequence data obtained by 454-pyrosequencing
    Magdalena Kröber
    Institut für Genomforschung und Systembiologie, Centrum für Biotechnology CeBiTec, Universitat Bielefeld, D 33615 Bielefeld, Germany
    J Biotechnol 142:38-49. 2009
    ..identical to 16S-rDNA database sequences derived from different anaerobic environments including digestors and bioreactors. Most of the bacterial 16S-rDNA sequences could be assigned to the phylum Firmicutes with the most abundant ..
  24. ncbi High immunogenic enterovirus 71 strain and its production using serum-free microcarrier Vero cell culture
    Chia Chyi Liu
    Institute of Biotechnology, Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan
    Vaccine 25:19-24. 2007
    ..Therefore, serum-free microcarrier culture is a valuable process for developing inactivated EV71 vaccines...
  25. pmc Perfused multiwell plate for 3D liver tissue engineering
    Karel Domansky
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA
    Lab Chip 10:51-8. 2010
    ..that fosters maintenance of 3D tissue cultures under constant perfusion and we have integrated multiple bioreactors into an array in a multiwell plate format. All bioreactors are fluidically isolated from each other...
  26. pmc Microscale distribution of populations and activities of Nitrosospira and Nitrospira spp. along a macroscale gradient in a nitrifying bioreactor: quantification by in situ hybridization and the use of microsensors
    A Schramm
    Max Planck Institute for Marine Microbiology, D 28359 Bremen, Germany
    Appl Environ Microbiol 65:3690-6. 1999
    ..Therefore, it was possible for the first time to estimate the cell-specific activity of Nitrosospira spp. and hitherto-uncultured Nitrospira-like bacteria in situ...
  27. pmc Occurrence of ammonia-oxidizing archaea in wastewater treatment plant bioreactors
    Hee Deung Park
    Environmental Engineering and Sciences Program, Stanford University, Stanford, CA 94305 2115, USA
    Appl Environ Microbiol 72:5643-7. 2006
    We report molecular evidence that ammonia-oxidizing archaea (AOA) occur in activated sludge bioreactors used to remove ammonia from wastewater...
  28. ncbi Perspectives of microbial oils for biodiesel production
    Qiang Li
    Department of Chemical Engineering, Tsinghua University, Beijing, 100084, People s Republic of China
    Appl Microbiol Biotechnol 80:749-56. 2008
    ..This review is covering the related research about different oleaginous microorganisms producing oils, and the prospects of such microbial oils used for biodiesel production are also discussed...
  29. ncbi Transgenic animal bioreactors
    L M Houdebine
    Unité de biologie du développement et biotechnologie, Institut National de la Recherche Agronomique, Jouy en Josas, France
    Transgenic Res 9:305-20. 2000
    ....
  30. ncbi Nitrite concentration influences the population structure of Nitrospira-like bacteria
    Frank Maixner
    , , Althanstrasse 14, A 1090 Vienna, Austria
    Environ Microbiol 8:1487-95. 2006
    ..Consistent with the observed localization patterns, a higher nitrite concentration selected for sublineage I but suppressed sublineage II Nitrospira...
  31. pmc In situ characterization of Nitrospira-like nitrite-oxidizing bacteria active in wastewater treatment plants
    H Daims
    , , 85350 Freising, Germany
    Appl Environ Microbiol 67:5273-84. 2001
    ..In contrast, no uptake by the Nitrospira-like bacteria of any of the carbon sources tested was observed under anoxic or anaerobic conditions...
  32. ncbi Abundance of ammonia-oxidizing bacteria and archaea in industrial and domestic wastewater treatment systems
    Yaohui Bai
    State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco Environmental Sciences, Chinese Academy of Sciences, Beijing, China
    FEMS Microbiol Ecol 80:323-30. 2012
    ..In addition, the dominant groups of AOB in the industrial WTSs were Nitrosomonas and Nitrosospira; the composition of AOA in the domestic WTSs was very similar, possibly due to the same source of raw sewage...
  33. ncbi Correlation of EPS content in activated sludge at different sludge retention times with membrane fouling phenomena
    Djamila Al-Halbouni
    Institute of Hygiene and Environmental Health, RWTH Aachen University, Pauwelsstr 30, D 52074 Aachen, Germany
    Water Res 42:1475-88. 2008
    In this study, activated sludge characteristics were studied with regard to membrane fouling in membrane bioreactors (MBRs) for two pilot plants and one full-scale plant treating municipal wastewater...
  34. pmc In situ analysis of nitrifying biofilms as determined by in situ hybridization and the use of microelectrodes
    S Okabe
    Department of Urban and Environmental Engineering, Faculty of Engineering, Hokkaido University, Sapporo 060 0813, Japan
    Appl Environ Microbiol 65:3182-91. 1999
    ....
  35. pmc Influence of environmental conditions on methanogenic compositions in anaerobic biogas reactors
    Dimitar Karakashev
    Environment and Resources DTU, Technical University of Denmark, Bygningstorvet, DK 2800 Kongens Lyngby, Denmark
    Appl Environ Microbiol 71:331-8. 2005
    ..The impact of the original inoculum used for the reactor start-up was also investigated by assessment of the present population in the reactor. The inoculum population appeared to have no influence on the eventual population...
  36. ncbi Experimental demonstration of chaotic instability in biological nitrification
    David W Graham
    School of Civil Engineering and Geosciences, Newcastle University, Newcastle upon Tyne, UK
    ISME J 1:385-93. 2007
    ..We conclude that nitrification is prone to chaotic behavior because of a fragile AOB-NOB mutualism, which must be considered in all systems that depend on this critical reaction...
  37. ncbi A biaxial rotating bioreactor for the culture of fetal mesenchymal stem cells for bone tissue engineering
    Zhi Yong Zhang
    Graduate Program in Bioengineering GPBE, National University of Singapore, Singapore
    Biomaterials 30:2694-704. 2009
    ..b>Bioreactors provide a manner in which this can be achieved...
  38. ncbi Development of a novel bioelectrochemical membrane reactor for wastewater treatment
    Yun Kun Wang
    Department of Chemistry, University of Science and Technology of China, Hefei 230026, China
    Environ Sci Technol 45:9256-61. 2011
    ..Results clearly indicate that this innovative system holds great promise for efficient treatment of wastewater and energy recovery...
  39. ncbi Scale-up of breast cancer stem cell aggregate cultures to suspension bioreactors
    Benjamin S Youn
    Pharmaceutical Production Research Facility PPRF, Schulich School of Engineering, University of Calgary, Calgary, Alberta, Canada
    Biotechnol Prog 22:801-10. 2006
    ..the ability of propagating this scarce population in a controlled and reproducible manner, within suspension bioreactors. A rigorous theoretical framework has been developed in order to understand and characterize the implications ..
  40. ncbi Integrated electromicrobial conversion of CO2 to higher alcohols
    Han Li
    Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA 90095, USA
    Science 335:1596. 2012
    ..The process integrates electrochemical formate production and biological CO(2) fixation and higher alcohol synthesis, opening the possibility of electricity-driven bioconversion of CO(2) to commercial chemicals...
  41. pmc Reduced differentiation efficiency of murine embryonic stem cells in stirred suspension bioreactors
    Jaymi T Taiani
    Department of Medical Science, Schulich School of Engineering, Schulich School of Engineering, University of Calgary, Calgary, Alberta, Canada
    Stem Cells Dev 19:989-98. 2010
    ..The initial aims of the present study were: (i) to use stirred suspension bioreactors to expand and differentiate ESCs into osteogenic and chondrogenic cell types and (ii) to explore if these ESC-..
  42. ncbi Human liver cell spheroids in extended perfusion bioreactor culture for repeated-dose drug testing
    Rui M Tostões
    ITQB UNL, Oeiras, Portugal
    Hepatology 55:1227-36. 2012
    ....
  43. ncbi Use of a continuous multistage bioreactor to mimic winemaking fermentation
    T Clement
    INRA, UMR Sciences pour l Oenologie, Montpellier Cedex, France
    Int J Food Microbiol 150:42-9. 2011
    ..It also allowed to uncouple the variations of environmental parameters and proved to be a promising tool to gain new insights into yeast metabolism during alcoholic fermentation...
  44. ncbi Biofouling control with bead-entrapped quorum quenching bacteria in membrane bioreactors: physical and biological effects
    Sang Ryoung Kim
    School of Chemical and Biological Engineering, Seoul National University, Seoul 151 744, Republic of Korea
    Environ Sci Technol 47:836-42. 2013
    ..a vessel was described and shown to be efficient and economically feasible for biofouling control in membrane bioreactors (MBRs)...
  45. pmc Scalable stirred-suspension bioreactor culture of human pluripotent stem cells
    Daniel E Kehoe
    Department of Chemical and Biological Engineering, State University of New York at Buffalo, Buffalo, New York 14260, USA
    Tissue Eng Part A 16:405-21. 2010
    ..the expansion and directed differentiation of human embryonic stem cells (hESCs) in scalable stirred-suspension bioreactors (SSBs) demonstrated that large-scale production of therapeutically useful hESC progeny is feasible with current ..
  46. ncbi Perfusion of 3D encapsulated hepatocytes--a synergistic effect enhancing long-term functionality in bioreactors
    Rui M Tostões
    Animal Cell Technology Laboratory, IBET ITQB UNL, Oeiras, Portugal
    Biotechnol Bioeng 108:41-9. 2011
    ..125 day(-1), for three operative weeks. Perfusion feeding led to a 10-fold improvement in albumin synthesis in bioreactors containing non-encapsulated hepatocyte spheroids; no significant improvement was observed in phase I drug ..
  47. ncbi Cluster randomized controlled trial of the plastic BioSand Water filter in Cambodia
    C E Stauber
    Institute of Public Health, Georgia State University, P O Box 3995, Atlanta, Georgia 30302 3995, United States
    Environ Sci Technol 46:722-8. 2012
    ..The study suggests the plastic BSF may play an important role in scaling up the distribution/implementation of the BSF, potentially improving water quality and health in the region...
  48. pmc Abrogation of E-cadherin-mediated cellular aggregation allows proliferation of pluripotent mouse embryonic stem cells in shake flask bioreactors
    Lisa Mohamet
    Core Technology Facility, Faculty of Medical and Human Sciences, The University of Manchester, Manchester, United Kingdom
    PLoS ONE 5:e12921. 2010
    ..However, each of these methods exhibits specific disadvantages, such as high cost, additional downstream processes or reduced cell doubling times...
  49. ncbi Optimization of microcarrier cell culture process for the inactivated enterovirus type 71 vaccine development
    Suh Chin Wu
    Department of Life Science, Institute of Biotechnology, National Tsing Hua University, Hsinchu, Taiwan
    Vaccine 22:3858-64. 2004
    ..These results constitute valuable information on the development of a large-scale microcarrier cell culture process for producing inactivated EV71 vaccine...
  50. ncbi A metaproteomic approach gives functional insights into anaerobic digestion
    F Abram
    Microbial Ecology Laboratory, Department of Microbiology, School of Natural Sciences, National University of Ireland, Galway, Ireland
    J Appl Microbiol 110:1550-60. 2011
    ..The objective of this work was to provide functional evidence of key metabolic pathways important for anaerobic digestion processes through the identification of highly expressed proteins in a mixed anaerobic microbial consortium...
  51. ncbi Ammonia-oxidizing communities in a highly aerated full-scale activated sludge bioreactor: betaproteobacterial dynamics and low relative abundance of Crenarchaea
    George F Wells
    Environmental Engineering and Science Program, Stanford University, Stanford, CA 94305, USA
    Environ Microbiol 11:2310-28. 2009
    Ammonia-oxidizing bacteria (AOB) have long been considered key to the removal of nitrogen in activated sludge bioreactors. Culture-independent molecular analyses have established that AOB lineages in bioreactors are dynamic, but the ..
  52. ncbi Plant seeds as bioreactors for recombinant protein production
    On Sun Lau
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520 8104, USA
    Biotechnol Adv 27:1015-22. 2009
    ..Significant advances in using seeds as bioreactors have occurred in the past decade, which include the first commercialized plant-derived recombinant protein...
  53. ncbi Metabolic flux analysis in mammalian cell culture
    Lake Ee Quek
    Australian Institute for Bioengineering and Nanotechnology AIBN, The University of Queensland, Brisbane QLD 4072, Australia
    Metab Eng 12:161-71. 2010
    ..Advances in both modeling and analytical techniques are bringing this challenge within sight...
  54. pmc Isoprenoid pathway optimization for Taxol precursor overproduction in Escherichia coli
    Parayil Kumaran Ajikumar
    Department of Chemical Engineering, Massachusetts Institute of Technology MIT, Cambridge, MA 02139, USA
    Science 330:70-4. 2010
    ..More broadly, the modular pathway engineering approach helped to unlock the potential of the MEP pathway for the engineered production of terpenoid natural products...
  55. ncbi Bioreactor performance: a more scientific approach for practice
    A Lübbert
    Institut für Bioengineering, Martin Luther Universitat Halle Wittenberg, Biozentrum, Weinbergweg 22, 06120, Halle Saale, Germany
    J Biotechnol 85:187-212. 2001
    ..However, as it is frequently inadequate for optimal performance, feedback control may also be employed. Relevant aspects of such performance optimization are discussed...
  56. ncbi Trends and challenges in the microbial production of lignocellulosic bioalcohol fuels
    Christian Weber
    Institute of Molecular Biosciences, Goethe University Frankfurt am Main, Max von Laue Str 9, 60438 Frankfurt am Main, Germany
    Appl Microbiol Biotechnol 87:1303-15. 2010
    ..and thereby being highly resistant to inhibitors and fermentation products, and easy to cultivate in huge bioreactors. In this review, we compare the properties of various microorganisms, bacteria and yeasts, as well as current ..
  57. ncbi An effective and simplified fed-batch strategy for improved 2,3-butanediol production by Klebsiella oxytoca
    Zhi Kui Nie
    State Key Laboratory of Materials Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No 5 Xinmofan Road, Nanjing, 210009, People s Republic of China
    Appl Biochem Biotechnol 163:946-53. 2011
    ..5 g/l, 1.37 g/(l•h), and 0.43 g/g obtained in glucose-feedback fed-batch strategy. This feeding strategy was simple and easy to operate and could be feasible for industrial 2,3-BD production in the future...
  58. ncbi Growth characteristic of anaerobic ammonium-oxidizing bacteria in an anaerobic biological filtrated reactor
    Kazuichi Isaka
    Hitachi Plant Engineering and Construction Co, Ltd, Kami Honga 537, Matsudo, Chiba, 271 0064, Japan
    Appl Microbiol Biotechnol 70:47-52. 2006
    ..Maintaining the ideal environment for the growth of anammox bacteria in the ABF reactor might lead to faster growth. This is the first report of the growth rate of anammox bacteria based on the direct counting of anammox bacteria...
  59. ncbi Methanosarcina: the rediscovered methanogen for heavy duty biomethanation
    Jo De Vrieze
    Laboratory of Microbial Ecology and Technology LabMET, Ghent University, Coupure Links 653, B 9000 Gent, Belgium
    Bioresour Technol 112:1-9. 2012
    ..8-1.0 units and acetate concentrations up to 15,000 mg CODL(-1). The possibilities of Methanosarcina sp. as key organisms in specific types of anaerobic digestion systems are demonstrated in this review...
  60. ncbi Citric acid production from glycerol-containing waste of biodiesel industry by Yarrowia lipolytica in batch, repeated batch, and cell recycle regimes
    Waldemar Rymowicz
    Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, Norwida 25, PL 50 375, Wroclaw, Poland
    Appl Microbiol Biotechnol 87:971-9. 2010
    ..Repeated batch culture remained stable for over 1000 h; the RB variant of 30% feed every 3 days showed the best results: 124.2 g l(-1) citric acid with a yield of 0.77 g g(-1) and a productivity of 0.85 g l(-1) h(-1)...
  61. ncbi Membrane biofouling in pilot-scale membrane bioreactors (MBRs) treating municipal wastewater: impact of biofilm formation
    Yuki Miura
    Department of Urban and Environmental Engineering, Graduate School of Engineering, Hokkaido University, North 13, West 8, Kita ku, Sapporo 060 8628, Japan
    Environ Sci Technol 41:632-8. 2007
    For more efficient control and prediction of membrane biofouling in membrane bioreactors (MBRs), a fundamental understanding of mechanisms of membrane biofouling is essential...
  62. ncbi Production of recombinant protein therapeutics in cultivated mammalian cells
    Florian M Wurm
    Laboratory of Cellular Biotechnology, Institute of Biological Engineering and Biotechnology, Faculty of Life Sciences, Swiss Federal Institute of Technology, 1015 Lausanne, Switzerland
    Nat Biotechnol 22:1393-8. 2004
    ..Recently, the productivity of mammalian cells cultivated in bioreactors has reached the gram per liter range in a number of cases, a more than 100-fold yield improvement over titers ..
  63. ncbi Bioreactors and bioprocessing for tissue engineering
    Anthony Ratcliffe
    Advanced Tissue Sciences, La Jolla, CA 92037, USA
    Ann N Y Acad Sci 961:210-5. 2002
    ..time, scale-up, storage, and sterility issues all need to be considered and incorporated into the design of bioreactors. Each tissue-engineered product will likely require individualized bioreactor design...
  64. ncbi Bioprocess monitoring
    Peter Harms
    Department of Chemical and Biochemical Engineering, UMBC, 1000 Hilltop Circle, Baltimore, MD 21250, USA
    Curr Opin Biotechnol 13:124-7. 2002
    ..In the near future, bioprocess optimization will change paradigms as massively parallel, fully instrumented bioreactors become available and high-throughput bioprocessing becomes a reality.
  65. ncbi Dependence of morphology on agitation intensity in fed-batch cultures of Aspergillus oryzae and its implications for recombinant protein production
    A Amanullah
    Centre for Bioprocess Engineering, School of Chemical Engineering, The University of Birmingham, Edgbaston, Birmingham B 15 2TT, UK
    Biotechnol Bioeng 77:815-26. 2002
    ....
  66. ncbi Effects of oxygen concentration on N-removal in an aerobic granular sludge reactor
    A Mosquera-Corral
    Department of Chemical Engineering, School of Engineering, Avda Lope Gómez de Marzoa s n E 15782 Santiago de Compostela, Galicia, Spain
    Water Res 39:2676-86. 2005
    ..A solution to obtain stable aerobic granular sludge at low oxygen concentrations is needed in order to make aerobic granular sludge reactors feasible in practice...
  67. ncbi Influence of loosely bound extracellular polymeric substances (EPS) on the flocculation, sedimentation and dewaterability of activated sludge
    X Y Li
    Department of Civil Engineering, Environmental Engineering Research Centre, The University of Hong Kong, Pokfulam Road, Hong Kong, China
    Water Res 41:1022-30. 2007
    ....
  68. ncbi Removal of pharmaceuticals and fragrances in biological wastewater treatment
    Adriano Joss
    Eawag, Ueberlandstr 133, 8600 Dubendorf, Switzerland
    Water Res 39:3139-52. 2005
    ..e. removal by sorption and biological transformation)...
  69. ncbi The membrane bioreactor: a novel tool to grow anammox bacteria as free cells
    Wouter R L van der Star
    Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628BC Delft, The Netherlands
    Biotechnol Bioeng 101:286-94. 2008
    ..The production of anammox bacteria in suspension with high purity and productivity makes the MBR a promising tool for the cultivation and study of anammox bacteria...
  70. ncbi Transgenic rabbits as therapeutic protein bioreactors and human disease models
    Jianglin Fan
    Laboratory of Cardiovascular Disease, Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba 305 8575, Japan
    Pharmacol Ther 99:261-82. 2003
    ..the achievements made thus far with transgenic rabbits, with emphasis on the application of these rabbits as human disease models and live bioreactors for producing human therapeutic proteins and on the recent progress in cloned rabbits.
  71. ncbi Stability of the ANAMMOX process in a gas-lift reactor and a SBR
    A Dapena-Mora
    Department of Chemical Engineering, School of Engineering, University of Santiago de Compostela, Avda Lope Gómez de Marzoa s n, E 15782 Santiago de Compostela, Spain
    J Biotechnol 110:159-70. 2004
    ..Further research is needed to understand this phenomenon and to optimise the corresponding strategies to counteract the flotation...
  72. ncbi Industrial production of amino acids by coryneform bacteria
    Thomas Hermann
    Degussa AG, Feed Additives, Research and Development Biotechnology, Kantstr 2, 33790 Halle Westf, Germany
    J Biotechnol 104:155-72. 2003
    ..Finally one focus of this review is on recent developments in downstream technology...
  73. ncbi Making recombinant proteins in animals--different systems, different applications
    Michael K Dyck
    Centre de Recherche en Biologie de la Reproduction, Dépt des Sciences Animals, Pavillon Paul Comtois, Cite Universitaire, Universite Laval, Sainte Foy, Quebec, Canada, G1K 7P4
    Trends Biotechnol 21:394-9. 2003
    Transgenic animal bioreactors represent a powerful tool to address the growing need for therapeutic recombinant proteins...
  74. ncbi Quorum sensing: a new biofouling control paradigm in a membrane bioreactor for advanced wastewater treatment
    Kyung Min Yeon
    School of Chemical and Biological Engineering, Seoul National University, Seoul 151 744, Korea
    Environ Sci Technol 43:380-5. 2009
    ..In this study, the concept of bacterial QS was applied to membrane bioreactors (MBRs) for advanced wastewater treatment as a new biofouling control paradigm...
  75. ncbi Evaluating the effect of dissolved oxygen on ammonia-oxidizing bacterial communities in activated sludge
    Hee Deung Park
    Department of Civil and Environmental Engineering, 1415 Engineering Drive, University of Wisconsin, Madison, WI 53706, USA
    Water Res 38:3275-86. 2004
    ..Only the train operated with DO concentrations below 1mg/L favored the establishment of a population of AOB related to the N. europaea lineage...
  76. ncbi Bacterial community structures in MBRs treating municipal wastewater: relationship between community stability and reactor performance
    Yuki Miura
    Department of Urban and Environmental Engineering, Graduate School of Engineering, Hokkaido University, North 13, West 8, 060 8628, Sapporo, Japan
    Water Res 41:627-37. 2007
    Bacterial community structures in pilot-scale conventional membrane bioreactors (CMBRs) and hybrid MBRs (HMBRs) which were combined with pre-coagulation/sedimentation were analyzed by polymerase chain reaction-denaturing gradient gel ..
  77. pmc Effect of biowaste sludge maturation on the diversity of thermophilic bacteria and archaea in an anaerobic reactor
    M Goberna
    Universitat Innsbruck, Institut fur Mikrobiologie, Technikerstrasse 25 d, 6020, Innsbruck, Austria
    Appl Environ Microbiol 75:2566-72. 2009
    ..We suggest that the environmental filter imposed by the saline conditions (20 g liter(-1)) selected a stable community of halotolerant and halophilic prokaryotes...
  78. ncbi Bioreactors for tissue engineering
    Huang Chi Chen
    Department of Chemical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan
    Biotechnol Lett 28:1415-23. 2006
    b>Bioreactors are essential in tissue engineering, not only because they provide an in vitro environment mimicking in vivo conditions for the growth of tissue substitutes, but also because they enable systematic studies of the responses of ..
  79. pmc Engineered microenvironments for controlled stem cell differentiation
    Jason A Burdick
    Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Tissue Eng Part A 15:205-19. 2009
    ..we discuss the environments for guiding stem cell function by an interactive use of biomaterial scaffolds and bioreactors, and focus on the interplay between molecular and physical regulatory factors...
  80. ncbi Medium optimization for hen egg white lysozyme production by recombinant Aspergillus niger using statistical methods
    R Gheshlaghi
    Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, N2L 3G1 Waterloo, Ontario, Canada
    Biotechnol Bioeng 90:754-60. 2005
    ..5 g L-1, and CaCl2.2H2O 0.5 g L-1. This medium was projected to produce, theoretically, 212 mg L-1 lysozyme. Using this medium, an experimental maximum lysozyme concentration of 209+/-18 mg L-1 verified the applied methodology...
  81. pmc Heterogeneity of Aspergillus niger microcolonies in liquid shaken cultures
    Charissa de Bekker
    Microbiology and Kluyver Centre for Genomics of Industrial Fermentation, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Appl Environ Microbiol 77:1263-7. 2011
    ..Our data imply that the protein production of A. niger can be improved in industrial fermentations by reducing the heterogeneity within the culture...
  82. ncbi Moss bioreactors producing improved biopharmaceuticals
    Eva L Decker
    Plant Biotechnology, Faculty of Biology, University of Freiburg, Schaenzlestr 1, D 79104 Freiburg, Germany
    Curr Opin Biotechnol 18:393-8. 2007
    ..patens was developed as a contained tissue culture system for recombinant protein production in photo-bioreactors. By targeted gene replacements, moss strains were created with non-immunogenic humanised glycan patterns...
  83. ncbi Enhanced 2,3-butanediol production by Klebsiella oxytoca using a two-stage agitation speed control strategy
    Xiao Jun Ji
    State Key Laboratory of Materials Oriented Chemical Engineering, College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing, People s Republic of China
    Bioresour Technol 100:3410-4. 2009
    ..5 g l(-1) with the yield of 0.478 g g(-1) and the productivity of 1.71 g l(-1)h(-1), which were 6.23%, 6.22% and 22.14% over the best results controlled by constant agitation speeds...
  84. ncbi Bone tissue engineering bioreactors: dynamic culture and the influence of shear stress
    Andrew B Yeatts
    Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA
    Bone 48:171-81. 2011
    ..Spinner flask, rotating wall bioreactors, and perfusion systems have all been the focus of experiments, and each system has advantages and disadvantages...
  85. ncbi Production of 2,3-butanediol from corncob molasses, a waste by-product in xylitol production
    Ailong Wang
    State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, People s Republic of China
    Appl Microbiol Biotechnol 87:965-70. 2010
    ..4%. The present study suggests that the low-cost corncob molasses could be used as an alternative substrate for the production of BD by K. pneumoniae SDM, as well as a potential carbon source for production of other high-value chemicals...
  86. ncbi Scalable production of influenza virus in HEK-293 cells for efficient vaccine manufacturing
    Audrey Le Ru
    Animal Cell Technology, National Research Council of Canada, 6100 Royalmount Avenue, Montreal, Quebec, Canada
    Vaccine 28:3661-71. 2010
    ..After virus adaptation and optimization of infection conditions, production in 3-L bioreactor resulted in titers of up to 10(9)IVP/mL demonstrating the scale-up potential of the process...
  87. ncbi Production of recombinant human erythropoietin/Fc fusion protein by genetically manipulated chickens
    Carlos Alberto Penno
    Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi ku, Fukuoka, 819 0395, Japan
    Transgenic Res 19:187-95. 2010
    ..These results indicate that transport of Fc fusion proteins from the blood circulation to the yolk in chickens represents an effective strategy for the production of pharmaceutical glycoproteins using transgenic chicken bioreactors.
  88. ncbi Plants as bioreactors for the production of vaccine antigens
    Siddharth Tiwari
    Plant Molecular Biology and Genetic Engineering Division, National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow 226001, U P, India
    Biotechnol Adv 27:449-67. 2009
    ..In the near future, a plethora of protein products, developed through 'naturalized bioreactors' may reach market...
  89. ncbi Microbial community analysis of a biogas-producing completely stirred tank reactor fed continuously with fodder beet silage as mono-substrate
    Michael Klocke
    Leibniz Institut für Agrartechnik Potsdam Bornim e V, Abteilung Bioverfahrenstechnik, Max Eyth Allee 100, D 14469 Potsdam Bornim, Germany
    Syst Appl Microbiol 30:139-51. 2007
    ..Members of the phyla Actinobacteria and Spirochaetes were represented only by 5 and 2 clonal sequences, respectively...
  90. ncbi Microdiesel: Escherichia coli engineered for fuel production
    Rainer Kalscheuer
    Institut fur Molekulare Mikrobiologie und Biotechnologie, Westfalische Wilhelms Universitat, Corrensstrasse 3, D 48149 Munster, Germany
    Microbiology 152:2529-36. 2006
    ..This novel approach might pave the way for industrial production of biodiesel equivalents from renewable resources by employing engineered micro-organisms, enabling a broader use of biodiesel-like fuels in the future...
  91. ncbi Plant-based production of biopharmaceuticals
    Rainer Fischer
    Institute for Molecular Biotechnology, Biology VII, RWTH Aachen, Worringerweg 1, 52074 Aachen, Germany
    Curr Opin Plant Biol 7:152-8. 2004
    ..There has also been significant success in tackling some of the limitations of plant bioreactors, such as low yields and inconsistent product quality, that have limited the approval of plant-derived ..
  92. ncbi The systematic production of cells for cell therapies
    Daniel C Kirouac
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON M5S 3E1, Canada
    Cell Stem Cell 3:369-81. 2008
    ..The step-wise implementation of these bioengineering strategies will enhance cell therapy product quality and safety, expediting clinical development...
  93. ncbi Contained and high-level production of recombinant protein in plant chloroplasts using a temporary immersion bioreactor
    Franck Michoux
    Division of Biology, Faculty of Natural Sciences, Imperial College London, London, UK
    Plant Biotechnol J 9:575-84. 2011
    ..platform, combining the rapid generation of transplastomic cell suspension cultures and the use of temporary immersion bioreactors, is a promising route for the fully contained low-cost production of recombinant proteins in chloroplasts.
  94. ncbi Identification and characterisation of novel Pichia pastoris promoters for heterologous protein production
    Gerhard Stadlmayr
    Department of Biotechnology, BOKU University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria
    J Biotechnol 150:519-29. 2010
    ....
  95. ncbi The EPS characteristics of sludge in an aerobic granule membrane bioreactor
    Wang Xuan
    Tianjin Key Laboratory of Hollow Fiber Membrane Material and Membrane Process, Institute of Biological and Chemical Engineering, Tianjin Polytechnical University, Tianjin 300160, China
    Bioresour Technol 101:8046-50. 2010
    ..Furthermore, application of aerobic granule can improve sludge filtering properties and delay the process of membrane fouling, as a result of better morphological structure and lower EPS content...
  96. ncbi Induced cooperation between marine nitrifiers and anaerobic ammonium-oxidizing bacteria by incremental exposure to oxygen
    Jia Yan
    Department of Microbiology, IWWR, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, Netherlands
    Syst Appl Microbiol 33:407-15. 2010
    ..The developed marine model system proved an effective tool to study the interactions between marine anammox bacteria and nitrifiers and their responses to changes in environmentally relevant conditions...
  97. ncbi Manufacturing recombinant proteins in kg-ton quantities using animal cells in bioreactors
    Maria De Jesus
    ExcellGene SA, Monthey, Switzerland
    Eur J Pharm Biopharm 78:184-8. 2011
    Mammalian cells in bioreactors as production host are the focus of this review...
  98. pmc Quantitative evaluation of yeast's requirement for glycerol formation in very high ethanol performance fed-batch process
    Julien Pagliardini
    Universite de Toulouse, INSA, UPS, INP, LISBP, 135 av de Rangueil, F 31077 Toulouse, France
    Microb Cell Fact 9:36. 2010
    ..Two engineered strains whose specific GPDH activity was significantly reduced by two different degrees were comprehensively characterized in a previously developed Very High Ethanol Performance (VHEP) fed-batch process...
  99. ncbi Modeling and design of optimal flow perfusion bioreactors for tissue engineering applications
    L Araida Hidalgo-Bastida
    Materials Science Centre, Manchester University, Manchester, UK
    Biotechnol Bioeng 109:1095-9. 2012
    Perfusion bioreactors have been used in different tissue engineering applications because of their consistent distribution of nutrients and flow-induced shear stress within the tissue-engineering scaffold...
  100. ncbi Balance of neutral and deterministic components in the dynamics of activated sludge floc assembly
    Joaquín M Ayarza
    Instituto de Investigaciones en Ingeniería Genética y Biología Molecular INGEBI CONICET, Buenos Aires, Argentina
    Microb Ecol 61:486-95. 2011
    ..and composition of bacterial communities on the dynamics of activated sludge floc assembly in lab-scale bioreactors. Bacterial community structure was analyzed using denaturing gradient gel electrophoresis of RT-PCR amplified ..
  101. pmc Metabolic and kinetic analyses of influenza production in perfusion HEK293 cell culture
    Emma Petiot
    Biotechnology Research Institute, 6100 Royalmount Avenue, Montreal, H4P 2R2 Quebec, Canada
    BMC Biotechnol 11:84. 2011
    ..In the same study, it was suggested that beyond 4 × 10(6) cells/mL, viral production was limited by a lack of nutrients or an accumulation of toxic products...

Research Grants81

  1. Oral immune modulatory therapy using antigens bioencapsulated in plant cells
    Roland W Herzog; Fiscal Year: 2013
    ..This proposal takes advantage of a unique concept based on the use of plant chloroplasts as highly efficient bioreactors for production and oral delivery of therapeutic protein antigens without a need for fermentation, purification, ..
  2. Chemokine Signals in the Premetastatic Niche Inhibit Metastasis
    Ann Richmond; Fiscal Year: 2013
    ..metastatic capacity of mammary carcinoma;2) Using immunocompetent mice, intravital imaging, and ex-vivo micro-bioreactors we will determine whether current therapeutics for breast cancer that inhibit PI3K are able to block or ..
  3. Liver Tissue Engineering Through Three-Dimensional Hepatocyte Culture
    Tammy T Chang; Fiscal Year: 2013
    ..independent research program based on my post- doctoral work on 3D hepatocyte culture in solid-body rotational bioreactors. Liver transplantation is currently the only treatment for patients with end-stage liver disease (ESLD), which ..
  4. Correlating and Quantifying Mechanically-induced Bone Cell Microdamage
    Marnie M Saunders; Fiscal Year: 2012
    ..osteonecrosis treatments;improve fracture healing and fixation of orthopedic and craniofacial implants;develop bioreactors to aid in functional tissue engineering of bone substitutes;and, develop new laboratory models and 'lab-on-..
  5. FAH-Deficient Pigs
    Scott L Nyberg; Fiscal Year: 2011
    ..These FAH deficient mice serve as in vivo bioreactors for the robust expansion of normal human hepatocytes...
  6. Bioprocess for cardiac cell generation from human induced pluripotent stem cells
    EMMANOUHL TZANAKAKIS; Fiscal Year: 2013
    ..Then, our second hypothesis is that hiPSCs cultured on microcarriers in stirred-suspension bioreactors can also be propagated to high concentrations...
  7. Discovery of genes that extend yeast lifespan: Aging in immobilized cell reactors
    RAPHAEL ROSENZWEIG; Fiscal Year: 2009
    ..Continuously or semi-continuously fed bioreactors populated by yeast immobilized in semi-solid beads can be maintained for weeks, or even months...
  8. PTHrP/IHH Microenvironment Control for Growth Plate Tissue Engineering
    JUAN MANUEL TABOAS; Fiscal Year: 2013
    ..We will create a novel model using microfluidic bioreactors and photopatterning of cells and hydrogels...
  9. Controlled Chemotaxis and Differentiation of Embryonic Cardiac Progenitor Cells
    Gregory M Fomovsky; Fiscal Year: 2013
    ..5 gene, in the controlled chemotactic environment of microfluidic bioreactors. The Nkx2...
  10. Three-dimensional Scaffold-based Systems for Primary Human Intestinal Culture
    Calvin J Kuo; Fiscal Year: 2013
    ..through the development of 3D scaffold-based systems for primary human intestinal culture within designed bioreactors. Our innovative approach combines expertise in intestinal biology with the engineering of cellular ..
  11. Matrix and Bioreactors for Human Lung Regeneration
    Eric S White; Fiscal Year: 2013
    ..We will also develop tools and bioreactors that will enable other Consortium members to utilize and leverage our findings...
  12. Elastogenic Factor Functionalized Electrospun Scaffolds for in vivo Generation of
    CHRISTOPHER A BASHUR; Fiscal Year: 2013
    ..to provide components of a physiologic microenvironment for cells, while circumventing the need with in vitro bioreactors to isolate primary cells from biopsied healthy tissues and expand them in culture prior to cell seeding...
  13. Elucidation of Leukocyte and Macrophage Biomarker Signatures from Drugs of Abuse
    JOHN PETER WIKSWO; Fiscal Year: 2010
    ..Duke and CFD Research Corporation, propose to merge their talents and optimize computer-controlled nanoliter bioreactors that would use optical, electrical, and electrochemical sensors, two-dimensional mass spectrometry, and novel ..
  14. Role of shear forces in proplatelet production from stem cells in bioreactors
    Eleftherios T Papoutsakis; Fiscal Year: 2012
    ..Ex vivo generation in bioreactors of functional platelets would have a major impact in transfusion medicine...
  15. Microbiome-Based Risk Assessment: Reciprocal Effects of Arsenic on Environmental
    Stephanie Bachas-Daunert; Fiscal Year: 2013
    ..of exogenous arsenic on the gut microbiome and on the resulting transformation of arsenic using anaerobic bioreactors in the absence/presence of arsenic-amended media...
  16. Innovative In Vivo-like Model for Vascular Tissue Engineering
    Eva Schmelzer; Fiscal Year: 2013
    ..We have already demonstrated that our 3D perfusion bioreactors support the spontaneous neo-tissue formation with neo-vascular hepatic structures and functionality in the ..
  17. Scalable bioprocess for cardiomyocyte generation from human embryonic stem cells
    EMMANOUHL TZANAKAKIS; Fiscal Year: 2009
    ..cell quantities can be generated under tightly controlled culture conditions in scalable stirred-suspension bioreactors. We have discovered that mouse ESCs cultivated in a bioreactor can be expanded several fold and differentiate ..
  18. Pharmacology and Bioengineering of New Treatment of ITP
    Joseph P Balthasar; Fiscal Year: 2010
    ..and evaluate a new immune complex therapy for ITP, and Aim #4 will optimize the construction of hollow fiber bioreactors for efficient and selective extracorporeal removal of pathogenic, antiplatelet antibodies...
  19. Biodegradable, hyaluronic acid-based microcarriers for mesenchymal stem cell prop
    ISAAC EDWARD ERICKSON; Fiscal Year: 2011
    ..Phase II research will focus on scaling up both the production of hMSCs on microcarriers in industrial scale bioreactors and of the microcarriers themselves in compliance with the cGMP requirements of the FDA...
  20. Determining the Effect of Altered Pulse Pressure on Artery Structure and Function
    DANIKA HAYMAN; Fiscal Year: 2010
    ..in pulse pressure, which will improve our understanding of vascular pathophysiology and provide useful guidance for the design of heart pumps and vascular bioreactors. Ultimately this knowledge will improve vascular disease treatment.
  21. Nanoparticle-mediated peptide delivery for breast cancer
    JORDAN JAMIESON GREEN; Fiscal Year: 2012
    ..Angiogenesis bioreactors implanted subcutaneously in mice will be used to monitor delivery of antiangiogenic peptides by the ..
  22. A Novel Plant Cell Bio-Production Platform for Therapeutic Proteins
    Jianfeng Xu; Fiscal Year: 2010
    ..of innovative more cost-effective methods for producing therapeutic proteins by using plant-based cultures and bioreactors which will confer the simultaneously benefits of greater safety, energy conservative and environmental ..
  23. Modulation of NF-kB Signaling by Immunoprobiotics
    James Versalovic; Fiscal Year: 2011
    ..b>Bioreactors with defined combinations of intestinal bacteria will be used in order to simulate gut bacterial ecosystems...
  24. GENE REGULATION AND PHYSIOLOGY OF STREPTOCOCCUS MUTANS
    Robert A Burne; Fiscal Year: 2013
    ..These studies utilize the most modern methods in molecular biology - coupled with the use of continuous-flow bioreactors to carefully control the physiology of the bacteria - to dissect how the source and the availability of ..
  25. DNA Sequencing and Nanotechnology
    Nyoté J Oliver; Fiscal Year: 2012
    ..The process of immobilizing enzymes to solid supports provides unique capabilities for bioreactors, including the facile separation of enzyme from products and in some cases, increased enzyme stability and ..
  26. Design and Evaluation of Ultrasound Stimulation Aided Bioreactor Configurations
    Anu Subramanian; Fiscal Year: 2010
    ..The long-term goal of our research is to design and develop bioreactors or bioprocessing units that can be used to generate engineered-tissues...
  27. Cardiac Progenitor Cell Chemotaxis
    Ovid C Amadi; Fiscal Year: 2013
    ..Microfluidic bioreactors will be used in these investigations...
  28. Biologically inspired engineering of hierarchical vascular networks
    George Eng; Fiscal Year: 2013
    ..Cardiac tissue will be grown around the vessel structure, using a hydrogel encapsulation method. Bioreactors will provide biophysical signaling using a time varying fluid flow regime to mature the blood vessels along ..
  29. Dynamically Responsive Bioreactors for Cartilage Regeneration
    Stephanie J Bryant; Fiscal Year: 2013
    ..Findings from this grant will lay the foundation for seeking competitively a NIH R01 and to pursue their (pre)clinical utility. ..
  30. ERIN CRC: Host-microbiota-pathogen interactions govern enteric health and disease
    Linda S Mansfield; Fiscal Year: 2013
    ..We will address these hypotheses using (1) bioreactors and mice colonized with human microbiota in Areas 1 and 2, and (2) samples from an epidemiological study of ..
  31. ASGSB/ISGP 2011 Joint Meeting
    Jeffrey R Alberts; Fiscal Year: 2011
    ..be represented by a forward-looking symposium addressing tissue engineering, synthetic biology, and the use of bioreactors. Submitted papers and posters will cover all these topics...
  32. Direct Stimulation of Bacterial Metabolism with Applied Electrical Current
    Drew Mackellar; Fiscal Year: 2013
    ..and improve the capacity of Shewanella cells to accept electrical current, using electrically active bioreactors in combination with microscopy of cells expressing fluorescent protein sensors, transcriptome sequencing, and ..
  33. Phase II Commercialization of Continuous Countercurrent Tangential Chromatography
    Oleg Shinkazh; Fiscal Year: 2013
    ..g.,in combination with single-use bioreactors. The overall goal of the proposed research is to design, construct and validate the first cGMP CCTC system...
  34. Organogenesis of Ectopic Tissue in Lymph Node
    Eric Lagasse; Fiscal Year: 2013
    ..to the development of complex 3- dimensional tissue models and a new paradigm by using lymph node as in vivo bioreactors to grow tissue or organ substitute...
  35. BIOREACTORS FOR TISSUES EXPOSED TO HIGH FREQUENCY VIBRATION
    Ingo R Titze; Fiscal Year: 2010
    This research addresses the need for innovative bioreactor design. Bioreactors are laboratory systems that precisely control mechanical forces and environmental conditions on biologically changing tissues for periods of several hours to ..
  36. Craniofacial Tissue Engineering
    Gordana Vunjak-Novakovic; Fiscal Year: 2013
    ..shaped cartilage/bone grafts formed using human stem cells, composite scaffolds and perfusion/loading bioreactors. From these studies, we published 74 peer-reviewed journal articles, 27 of which were coauthored by the two ..
  37. A Mass Spectrometry Probe for Transient Imaging of In-Solution Biochemistry
    Andrei G Fedorov; Fiscal Year: 2011
    ..role in biological research as a hypothesis generator, and will become a key tool in improving development of bioreactors for regenerative medicine applications...
  38. Gap Junction and Bone Cell Responses to Physical Signals
    Henry J Donahue; Fiscal Year: 2010
    ..Additionally, an understanding of how mechanical signals regulate bone cell proliferation and differentiation would be beneficial in designing in vitro environments, i.e. bioreactors, for novel bone tissue engineering protocols.
  39. A high-growth PR8 virus for pandemic vaccine production in ST6-Vero cells
    Yoshihiro Kawaoka; Fiscal Year: 2012
    ..ST6-Vero also may be grown to highly packed cell densities in commercial bioreactors thereby providing more host cells for viral replication...
  40. Tissue Engineering of Hematopoietic Bone
    Karen Hirschi; Fiscal Year: 2009
    ..Goodell, BCM), bone development (Davis, BCM), vascular development (Hirschi, BCM &Rice), biomaterials and bioreactors (West and Mikos, Rice) and bioimaging (Barry and Sevick, BCM and Rice)...
  41. Nanofiber based artificial nerve graft for peripheral nerve regeneration
    Xiaojun Yu; Fiscal Year: 2010
    ..proposal is the development of nanofiber based biodegradable nerve grafts pre- seeded with Schwann cells in bioreactors for enhancing peripheral nerve regeneration to the level comparable to autograft...
  42. Neuro-Vascular Regeneration
    Karen Hirschi; Fiscal Year: 2009
    ..We have designed and generated biomaterials and bioreactors that will enable stem cell survival and propagation and fabrication of perfused microvascular networks...
  43. A cost-effective bioreactor to advance functional tissue engineering of cartilage
    Michael Bottlang; Fiscal Year: 2012
    ..will be tested in the high- throughput bioreactor and a conventional single-station test system to validate the bioreactors automated measurement of mechanical properties...
  44. Enabling Strategies for Growing Collagenous Tissues
    LAURA E contact NIKLASON; Fiscal Year: 2010
    ..In this application, we will exploit and develop novel mathematical models, bioreactors, and non- invasive imaging systems to predict, track and quantify the deposition of extracellular matrix in ..
  45. A Mass Spectrometry Probe for Transient Imaging of In-Solution Biochemistry
    Andrei G Fedorov; Fiscal Year: 2013
    ..role in biological research as a hypothesis generator, and will become a key tool in improving development of bioreactors for regenerative medicine applications...
  46. PET and MR-Compatible Bioreactor for Cross-Platform Biomarker Development
    Henry F VanBrocklin; Fiscal Year: 2013
    ..Three-dimensional (3D) cell and tissue culture bioreactors provide a novel platform to investigate, in a very controlled and cost effective environment, cell and tissue ..
  47. Human Tissue Culture Bioreactor and Hyperpolarized MR for Biomarker Discovery
    Kayvan R Keshari; Fiscal Year: 2012
    ..research program focused on the bioengineering and implementation of novel 3D cell and tissue culture bioreactors, and the use this platform in conjunction with hyperpolarized (HP) 13C MR to better study cancer metabolism...
  48. High-Expression, Rapid Production of Influenza Vaccines in Cell-Based Systems
    Vitaliy Gavrilyuk; Fiscal Year: 2013
    ..Aim 3) Develop scalable bioprocess protocols for influenza virus production in bench top and industrial bioreactors (50-100L);Adapted cells and optimal conditions from Aim 2 will be used in small-scale bench top bioreactors to ..
  49. A Novel 3-D System for Cost-Effective Industrial Production of Pluripotent Cells
    Bradley H Garcia; Fiscal Year: 2013
    ..system for robust expansion of induced pluripotent stem cells (iPSCs) by a) scaling up iPSC production in 3L bioreactors, b) validating the bioreactor-expanded iPSCs for differentiatation into lineages of value for pharmaceutical ..
  50. Human Tissue Culture Bioreactor and Hyperpolarized MR for Biomarker Discovery
    Kayvan R Keshari; Fiscal Year: 2013
    ..research program focused on the bioengineering and implementation of novel 3D cell and tissue culture bioreactors, and the use this platform in conjunction with hyperpolarized (HP) 13C MR to better study cancer metabolism...
  51. Regenerating Blood Vessels Using iPS Cells
    Peter X Ma; Fiscal Year: 2013
    ..mechanistic understandings and advanced technologies, we will engineer blood vessels and evaluate them using bioreactors and a rat implantation model...
  52. Nanotechnology in Biosensors and Bioengineering
    Yogesh K Vohra; Fiscal Year: 2011
    ..Areas for training include nanostructured biomaterials for dental, orthopedic, and cardiovascular implants, bioreactors for nanostructured scaffolds for tissue engineering, cell biology on nanoscale materials, and optical ..
  53. PREDOCTORAL TRAINING PROGRAM IN BIOTECHNOLOGY
    DANIEL M QUINN; Fiscal Year: 2013
    ..careers: a) at least one course in each of the three core areas of biotechnology (Biocatalysis, Bioreactors &Bioseparations, Molecular Biology &Genetics);b) participation in the interdisciplinary seminar "..
  54. Biosynthesis of Taxol Precursors in Transgenic Moss
    Aldwin Anterola; Fiscal Year: 2009
    ..to genetic manipulation, grows well photoautotrophically in culture, is already being used in industrial bioreactors, and is a plant cell, which altogether suggest a reasonable hypothesis that this moss is by far the best ..
  55. Tissue Engineering Resource Center
    David L Kaplan; Fiscal Year: 2013
    ..by applicant): The P41 Tissue Engineering Resource Center (TERC) has established innovative biomaterials, bioreactors, and tissue engineering models since its inception in August 2004...
  56. Microcarrier / CEF / Media SYSTEM to make Viral Vaccines
    WILLIAM HILLEGAS; Fiscal Year: 2002
    ..in which (1) primary CEF cells are utilized in roller bottles, (2) embryonated, fertile eggs function as tiny bioreactors, such as in the manufacturing of human influenza vaccine or (3) whole cell vaccines and/or immunotherapies...
  57. Botulinum Toxin Plantibodies
    KEITH WYCOFF; Fiscal Year: 2002
    ..plants will facilitate the production of metric ton quantities of antibodies at 1-5% of the cost of steel tank bioreactors. Plantibody technology will greatly reduce the costs to stockpile antibodies for protection of the population ..
  58. Enhanced Oxygenation of Bioreactors
    Stuart Nemser; Fiscal Year: 2003
    ..will significantly enhance bioreactor product Mty by significantly increasing the bubbleless oxygen delivery to bioreactors. Sales of major biopharmaceuticals currently exceed $18 billion...
  59. Recombinant human serum albumin for use in cell culture media
    Ning Huang; Fiscal Year: 2007
    ..will lead us to scale-up in Phase II, which will be to determine the growth of cells in the presence of rHSA in bioreactors and assess the ability of cells to produce vaccines...
  60. Plug and Play Bioreactor
    Gordana Vunjak-Novakovic; Fiscal Year: 2012
    ..However, major limitations remain. There is a need for bioreactors that would combine several critical requirements: (i) provision of precisely controlled 3D environments ..
  61. Novel 3-D Model of Placental Cytomegalovirus Infection
    Cindy Morris; Fiscal Year: 2006
    ..The culture conditions of these bioreactors provide a low-shear and low-turbulence growth environment, similar to that required for normal in vivo fetal ..
  62. MEMBRANE-BASED PRECONCENTRATORS FOR BIOMOLECULES
    James Cox; Fiscal Year: 1991
    ..term goals include the development of improved microdialysis systems for in vivo samplers, membrane-based units for the continuous recovery of targeted species from bioreactors, and flow-systems for monitoring and treating wastewater.
  63. A High-Performance Food Pathogen Detection Instrument
    Xiaoli Su; Fiscal Year: 2006
    ..instrument is fully integrated and includes a cartridge containing multiple capillary column-based bioseparator/bioreactors (for pathogen capture), and a liquid core waveguide (LCW) optical detector (for pathogen detection)...
  64. Expression of Human Therapeutic Proteins in Transgenic Tobacco Chloroplasts
    Henry Daniell; Fiscal Year: 2010
    ..Successful completion of these studies would make transgenic chloroplasts ideal bioreactors for the production of safe and less expensive therapeutic proteins and open the door for in depth studies of ..
  65. DETERMINATION OF OPIOID PEPTIDES BY ELECTROSPRAY LC/MS
    Robert Voyksner; Fiscal Year: 1991
    ..specificity for compound identification, the proposed program will investigate the use of ilmnobilized enzyme bioreactors and tandem MS...
  66. Immunotherapy for Pulmonary Anthrax
    KEITH WYCOFF; Fiscal Year: 2003
    ..the production of metric ton quantities of an anthrax-protective protein at 1-5% of the cost of steel tank bioreactors. Plantibody technology will greatly reduce the costs to stockpile these proteins for protection of the entire ..
  67. A MICROCARRIER/CEF/MEDIA SYSTEM TO MAKE VIRAL VACCINES
    WILLIAM HILLEGAS; Fiscal Year: 2000
    ..proposed is aimed at developing a system for growing chick embryo fibroblast (CEF) cells on microcarriers in bioreactors for the large- scale production of avian and human virus vaccines...
  68. HYPERBILIRUBINEMIA TREATMENT WITH CLONED THERMO-ENZYMES
    Larry Allen; Fiscal Year: 1993
    DESCRIPTION (Adapted from applicant's abstract):ThermoGen proposes the development of bioreactors and injectable drugs for the treatment of jaundice. These will be based on cloned thermophilic bilirubin oxidizing enzyme...
  69. PARATHYROID IMAGING BY RADIOLABELED MONOCLONAL ANTIBODY
    WILLIAM TOLBERT; Fiscal Year: 1990
    ..has specificity for antigens on the surface of parathyroid tissue, and can be mass produced in proprietary bioreactors by Invitron Corporation...
  70. Layered Manufacture of Bioactive Porous TE Scaffolds
    Malcolm Cooke; Fiscal Year: 2009
    ..Scaffolds will be manufactured using stereolithography, seeded with rat MSCs, and incubated in perfusion bioreactors for 4, 8 and 12 days...
  71. Development of an interferon-alpha-veltuzumab conjugate for CD20-targeted therapy
    EDMUND ROSSI; Fiscal Year: 2009
    ..the second aim, suitable host cell lines will be developed for production of clinical material in small-scale bioreactors by screening of 150-300 transgenic myeloma clones and subsequent amplification with methotrexate...
  72. Computational model of cellular adhesion in bulk flows
    CHARLES EGGLETON; Fiscal Year: 2009
    ..among others, to target drug delivery via the circulatory system, grow self-assembling tissue structures in bioreactors, and miniaturize biosensors for the detection of environmental bacteria...
  73. POSTINCORPORATION DYE LABELING FOR DNA SEQUENCING
    Wolfgang Kusser; Fiscal Year: 1999
    ..to complete the development and testing of an improved optical oxygen sensor for dissolved oxygen monitoring in bioreactors. The improved sensor, based on measurements of fluorescence lifetime quenching, will facilitate reliable, long-..
  74. Epithelially-derived factors in interstitial cystitis
    John Hanrahan; Fiscal Year: 2004
    ..Bladder epithelial cells will also be cultured in hollow fiber bioreactors so that other epithelially-derived factors that may be co-released with ATP can be collected and identified...
  75. NOVEL E1 CELL LINE FOR RCA-FREE ADENOVIRAL GENE THERAPY
    Richard Peluso; Fiscal Year: 2001
    ..3) utilizes an E1 helper cell line, recognized as safe by the FDA, which can be readily scaled to >10 L bioreactors. PROPOSED COMMERCIAL APPLICATION: Genovo is using adenoviral vectors expressing the beta-interferon gene for ..
  76. In situ Optical Analyzer for Components in Bioreactors
    RONALD MICHEELS; Fiscal Year: 2002
    An optically based instrument for in-situ analysis of multiple chemical components in bioreactors will be developed and applied to monitor key components in hybridoma cell fermentation processed used for producing monoclonal antibodies...
  77. 3-D Organotypic Model of HIV-infectable Tissue for AIDS-associated dementia
    CHERYL NICKERSON; Fiscal Year: 2009
    ..Rotating wall vessel (RWV) bioreactors will be used to establish this advanced 3-D tissue model system...
  78. Production of cell lines with improved reprogramming potential for SCNT
    Rachel Power; Fiscal Year: 2009
    ..cell reprogramming technology to improve livestock cloning methods include high genetic merit livestock, animal bioreactors for large scale production of therapeutic molecules, and development of animal models of disease...
  79. Bioreactor for Engineered Bioartificial Tissues (BATS)
    Albert Banes; Fiscal Year: 2005
    Few commercial bioreactors are available for the culture of three dimensional (3D) tissues...
  80. MRI AND MRS OF MODEL STROKE IN PERFUSED CELL CULTURES
    Theodore Trouard; Fiscal Year: 2001
    ..Ischemic conditions can be experimentally induced within bioreactors and the cellular response can be studied in detail using MRI and magnetic resonance spectroscopy (MRS) methods...
  81. Integral membrane protein overexpression using organ bioreactors
    Paul Allen; Fiscal Year: 2009
    ..native" organ expression or can be used to inducibly "over-express" IMPs in muscle and liver as in vivo organ bioreactors. 2...