specimen handling

Summary

Summary: Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.

Top Publications

  1. pmc HPV testing on self collected cervicovaginal lavage specimens as screening method for women who do not attend cervical screening: cohort study
    Murat Gok
    Department of Pathology, VU University Medical Center, PO Box 7057, 1007 MB Amsterdam, Netherlands
    BMJ 340:c1040. 2010
  2. ncbi Use of filter paper for the collection and analysis of human whole blood specimens
    J V Mei
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA
    J Nutr 131:1631S-6S. 2001
  3. pmc Dried blood spots as a source of anti-malarial antibodies for epidemiological studies
    Patrick H Corran
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
    Malar J 7:195. 2008
  4. pmc Pooled analysis of a self-sampling HPV DNA Test as a cervical cancer primary screening method
    Fang Hui Zhao
    Department of Epidemiology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People s Republic of China
    J Natl Cancer Inst 104:178-88. 2012
  5. pmc Long-term storage limits PCR-based analyses of malaria parasites in archival dried blood spots
    Joyce Hwang
    Shoklo Malaria Research Unit, Mae Sot, Tak Province, Thailand
    Malar J 11:339. 2012
  6. ncbi Comparison of nasopharyngeal flocked swabs and aspirates for rapid diagnosis of respiratory viruses in children
    K H Chan
    Department of Microbiology, The University of Hong Kong and Queen Mary Hospital, Hong Kong SAR, China
    J Clin Virol 42:65-9. 2008
  7. ncbi Preanalytical quality improvement: from dream to reality
    Giuseppe Lippi
    Clinical Chemistry and Hematology Laboratory, Academic Hospital of Parma, Parma, Italy
    Clin Chem Lab Med 49:1113-26. 2011
  8. ncbi Women find it easy and prefer to collect their own vaginal swabs to diagnose Chlamydia trachomatis or Neisseria gonorrhoeae infections
    Max A Chernesky
    St Joseph s Healthcare, Hamilton, Ontario, Canada
    Sex Transm Dis 32:729-33. 2005
  9. ncbi Impact of blood collection devices on clinical chemistry assays
    Raffick A R Bowen
    Department of Pathology, Stanford University, 300 Pasteur Drive, Room H1507 B, Stanford, CA 94305, USA
    Clin Biochem 43:4-25. 2010
  10. pmc Impact of biospecimens handling on biomarker research in breast cancer
    Loris De Cecco
    Fondazione Istituto FIRC di Oncologia Molecolare, Via Adamello16, 20139 Milano, Italy
    BMC Cancer 9:409. 2009

Research Grants

Detail Information

Publications289 found, 100 shown here

  1. pmc HPV testing on self collected cervicovaginal lavage specimens as screening method for women who do not attend cervical screening: cohort study
    Murat Gok
    Department of Pathology, VU University Medical Center, PO Box 7057, 1007 MB Amsterdam, Netherlands
    BMJ 340:c1040. 2010
    ..To determine whether offering self sampling of cervicovaginal material for high risk human papillomavirus (HPV) testing is an effective screening method for women who do not attend regular cervical screening programmes...
  2. ncbi Use of filter paper for the collection and analysis of human whole blood specimens
    J V Mei
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA
    J Nutr 131:1631S-6S. 2001
    ..These activities have created a mechanism for the validation of the filter paper blood collection device and the standardization of materials and methods for the analysis of dried blood spots...
  3. pmc Dried blood spots as a source of anti-malarial antibodies for epidemiological studies
    Patrick H Corran
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
    Malar J 7:195. 2008
    ..Although recommendations for the storage and analysis of small molecule analytes in blood spots exist, there are no published systematic studies of the stability of antibodies under different storage conditions...
  4. pmc Pooled analysis of a self-sampling HPV DNA Test as a cervical cancer primary screening method
    Fang Hui Zhao
    Department of Epidemiology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People s Republic of China
    J Natl Cancer Inst 104:178-88. 2012
    ....
  5. pmc Long-term storage limits PCR-based analyses of malaria parasites in archival dried blood spots
    Joyce Hwang
    Shoklo Malaria Research Unit, Mae Sot, Tak Province, Thailand
    Malar J 11:339. 2012
    ..However, the suitability of the template obtained from dried blood spots that have been stored for long periods for DNA amplification is not known...
  6. ncbi Comparison of nasopharyngeal flocked swabs and aspirates for rapid diagnosis of respiratory viruses in children
    K H Chan
    Department of Microbiology, The University of Hong Kong and Queen Mary Hospital, Hong Kong SAR, China
    J Clin Virol 42:65-9. 2008
    ..The quality of clinical specimens is a crucial determinant for virological diagnosis...
  7. ncbi Preanalytical quality improvement: from dream to reality
    Giuseppe Lippi
    Clinical Chemistry and Hematology Laboratory, Academic Hospital of Parma, Parma, Italy
    Clin Chem Lab Med 49:1113-26. 2011
    ....
  8. ncbi Women find it easy and prefer to collect their own vaginal swabs to diagnose Chlamydia trachomatis or Neisseria gonorrhoeae infections
    Max A Chernesky
    St Joseph s Healthcare, Hamilton, Ontario, Canada
    Sex Transm Dis 32:729-33. 2005
    ..We surveyed women's opinions on ease and preferences as to sampling after collecting their own vaginal swab and urine and a physician collection of vaginal swab and cervical swab...
  9. ncbi Impact of blood collection devices on clinical chemistry assays
    Raffick A R Bowen
    Department of Pathology, Stanford University, 300 Pasteur Drive, Room H1507 B, Stanford, CA 94305, USA
    Clin Biochem 43:4-25. 2010
    ..This review will identify sources of exogenous substances in blood specimens and propose methods to minimize their impact on clinical chemistry assays...
  10. pmc Impact of biospecimens handling on biomarker research in breast cancer
    Loris De Cecco
    Fondazione Istituto FIRC di Oncologia Molecolare, Via Adamello16, 20139 Milano, Italy
    BMC Cancer 9:409. 2009
    ....
  11. ncbi Biological sample collection and processing for molecular epidemiological studies
    Nina T Holland
    School of Public Health, University of California, Berkeley, CA 94720 7360, USA
    Mutat Res 543:217-34. 2003
    ....
  12. pmc Effects of tissue handling on RNA integrity and microarray measurements from resected breast cancers
    Christos Hatzis
    Nuvera Biosciences, Woburn, MA, USA
    J Natl Cancer Inst 103:1871-83. 2011
    ..Reliable stability and yield of RNA from breast cancer tissues are important for biobanking, clinical trials, and diagnostic testing...
  13. ncbi Are self-collected samples comparable to physician-collected cervical specimens for human papillomavirus DNA testing? A systematic review and meta-analysis
    Patrick Petignat
    Senology and Gynaecologic Oncology Unit, Geneva University Hospitals, 1211 Geneva 14, Switzerland
    Gynecol Oncol 105:530-5. 2007
    ..To compare the detection rate of genital human papillomavirus (HPV) infection in self- and physician-obtained samples...
  14. ncbi Comparison of a commercial qualitative real-time RT-PCR kit with direct immunofluorescence assay (DFA) and cell culture for detection of influenza A and B in children
    Farhad Gharabaghi
    Division of Microbiology, Department of Paediatric Laboratory Medicine, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, M5G 1X8 Canada
    J Clin Virol 42:190-3. 2008
    ..Institutional pandemic planning prompted a study of the molecular detection of influenza virus from respiratory specimens in children, compared to conventional diagnostics...
  15. ncbi Fatty acid status determination by cheek cell sampling combined with methanol-based ultrasound extraction of glycerophospholipids
    Mario Klingler
    Division of Metabolic and Nutritional Medicine, Dr von Hauner Children s Hospital, University of Munich Medical Center, Lindwurmstr 4, 80337, Munich, Germany
    Lipids 46:981-90. 2011
    ..The accuracy and reliability of this new method is comparable to conventional invasive methods for the determination of the n-3 fatty acid status in humans, and it is well applicable in interventional or epidemiological studies...
  16. ncbi Qualification of high-recovery, flocked swabs as compared to traditional rayon swabs for microbiological environmental monitoring of surfaces
    Giblerto Dalmaso
    GSK Manufacturing SpA, Strada Asolana n 68, San Polo di Torrile, 43056 Parma, Italy
    PDA J Pharm Sci Technol 62:191-9. 2008
    ..microRheologics flocked swabs also displayed an instant and nearly complete release of absorbed samples of more than 80%...
  17. ncbi Elimination of false-positive polymerase chain reaction results resulting from hole punch carryover contamination
    Nicolai Bonne
    School of Veterinary and Biomedical Sciences, South Street, Murdoch, Western Australia 6150
    J Vet Diagn Invest 20:60-3. 2008
    ..This method was simple, cheap, and relatively rapid, and allowed for the use of a single hole punch for a series of samples, without carryover contamination and consequent false-positive results...
  18. ncbi Preanalytical aspects and sample quality assessment in metabolomics studies of human blood
    Peiyuan Yin
    CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China
    Clin Chem 59:833-45. 2013
    ..Although excellent sample quality is essential, it can easily be compromised by undetected preanalytical errors. We set out to identify critical preanalytical steps and biomarkers that reflect preanalytical inaccuracies...
  19. pmc Equivalence of self- and staff-collected nasal swabs for the detection of viral respiratory pathogens
    Manas K Akmatov
    Department of Epidemiology, Helmholtz Centre for Infection Research, Braunschweig, Germany
    PLoS ONE 7:e48508. 2012
    ..This may be circumvented by having the participants collect their own nasal swabs. We compared self- and staff-collected swabs in terms of swabbing quality and detection of viral respiratory pathogens...
  20. ncbi Causes, consequences, detection, and prevention of identification errors in laboratory diagnostics
    Giuseppe Lippi
    University of Verona, Verona, Italy
    Clin Chem Lab Med 47:143-53. 2009
    ..Once misidentification is detected, rejection and recollection is the most suitable approach to manage the specimen...
  21. pmc Home versus clinic-based specimen collection for Chlamydia trachomatis and Neisseria gonorrhoeae
    Anna S Graseck
    Washington University in St Louis School of Medicine, Division of Clinical Research, Department of Obstetrics and Gynecology, Campus Box 8219, 4533 Clayton Avenue, St Louis, MO 63110, USA
    Expert Rev Anti Infect Ther 9:183-94. 2011
    ..This article describes and evaluates the effectiveness and limitations of various home screening strategies used for the detection of STIs...
  22. pmc Novel nested direct PCR technique for malaria diagnosis using filter paper samples
    Hans Peter Fuehrer
    Department of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Kinderspitalgasse 15, A 1090 Vienna, Austria
    J Clin Microbiol 49:1628-30. 2011
    ..Direct PCR provides a rapid, highly sensitive, and cost-effective alternative to diagnosing malaria using filter paper samples and standard nested PCR...
  23. ncbi Population-based outreach for Chlamydia screening in men: results from a randomized trial
    Delia Scholes
    Center for Health Studies, Group Health Cooperative, Seattle, WA 98101, USA
    Sex Transm Dis 34:837-9. 2007
    ..To evaluate the feasibility and efficacy of population-based outreach strategies to improve genital Chlamydia trachomatis (CT) screening in men...
  24. ncbi Does prevention of intraperitoneal spillage when removing a dermoid cyst prevent granulomatous peritonitis?
    W Kondo
    Department of Obstetrics and Gynaecology, centre hospitalier universitaire de Clermont Ferrand, Polyclinique de l Hotel Dieu, Clermont Ferrand, France
    BJOG 117:1027-30. 2010
    ....
  25. pmc Self-collected mid-turbinate swabs for the detection of respiratory viruses in adults with acute respiratory illnesses
    Oscar E Larios
    Department of Medicine, Royal University Hospital, Saskatoon, Saskatchewan, Canada
    PLoS ONE 6:e21335. 2011
    ..The objective of this study was to compare the respiratory virus isolation of flocked MT swabs compared to flocked NP swabs...
  26. ncbi DNA yield and quality of saliva samples and suitability for large-scale epidemiological studies in children
    A C Koni
    College of Medicine, Veterinary and Life Sciences, Institute of Cardiovascular and Medical Sciences, University of Glasgow, Scotland, UK
    Int J Obes (Lond) 35:S113-8. 2011
    ..To evaluate two saliva collection methods for DNA yield and quality as applied to a large, integrated, multicentre, European project involving the collection of biological material from children...
  27. pmc Added value of an oropharyngeal swab in detection of viruses in children hospitalized with lower respiratory tract infection
    Laura L Hammitt
    KEMRI Wellcome Trust Research Programme, Kilifi, Kenya
    J Clin Microbiol 49:2318-20. 2011
    ..Oropharyngeal swabs increased the number of viral infections detected by 15%, compared to collection of a nasopharyngeal swab alone. This advantage was most pronounced for detection of influenza, parainfluenza, and adenovirus...
  28. ncbi The potential role of self-sampling for high-risk human papillomavirus detection in cervical cancer screening
    Channa E Schmeink
    Department of Obstetrics and Gynaecology, Radboud University Nijmegen Medical Centre, The Netherlands
    Rev Med Virol 21:139-53. 2011
    ..Even more, because self-collected samples are as good as physician-collected samples for HPV detection, self-sampling might be a suitable method for future primary cervical cancer screening...
  29. pmc Effect of storage conditions on the assessment of bacterial community structure in soil and human-associated samples
    Christian L Lauber
    Cooperative Institute for Research in Environmental Sciences, University of Colorado, Boulder, CO 80309 0216, USA
    FEMS Microbiol Lett 307:80-6. 2010
    ..These results suggest that many samples collected and stored under field conditions without refrigeration may be useful for microbial community analyses...
  30. ncbi Liquid compared with conventional cervical cytology: a systematic review and meta-analysis
    Marc Arbyn
    Scientific Institute of Public Health, Brussels, Belgium
    Obstet Gynecol 111:167-77. 2008
    ..To compare test performance characteristics of conventional Pap tests and liquid-based cervical cytology samples...
  31. ncbi New views of cells in 3D: an introduction to electron tomography
    Richard McIntosh
    Laboratory for 3D Electron Microscopy of Cells, Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Trends Cell Biol 15:43-51. 2005
    ..Electron tomography can provide such information about many kinds of cells and organelles...
  32. pmc A multi-country non-inferiority cluster randomized trial of frontloaded smear microscopy for the diagnosis of pulmonary tuberculosis
    Luis Eduardo Cuevas
    Liverpool School of Tropical Medicine, Liverpool, United Kingdom
    PLoS Med 8:e1000443. 2011
    ..More than 50 million people around the world are investigated for tuberculosis using sputum smear microscopy annually. This process requires repeated visits and patients often drop out...
  33. pmc Real-time PCR detection of Plasmodium directly from whole blood and filter paper samples
    Brian J Taylor
    Department of Oncology, University of Alberta, Edmonton, Canada
    Malar J 10:244. 2011
    ..However, prior purification of genomic DNA from blood is necessary since PCR inhibitors and quenching of fluorophores from blood prevent efficient amplification and detection of PCR products...
  34. ncbi Fecal collection, ambient preservation, and DNA extraction for PCR amplification of bacterial and human markers from human feces
    Jordan M Nechvatal
    Department of Physiology, Wayne State University, 540 E Canfield Avenue, Detroit, MI 48201, USA
    J Microbiol Methods 72:124-32. 2008
    ..Thus, fecal DNA is well preserved by methods suitable for self-collection that may be useful in future molecular epidemiological studies of intestinal bacteria and human cancer markers...
  35. pmc Comparison of different culture media and storage temperatures for the long-term preservation of Streptococcus pneumoniae in the tropics
    G Siberry
    Department of Paediatrics, Johns Hopkins Medical Institutions, 600 N Wolfe Street, Baltimore, MD 21287, USA
    Bull World Health Organ 79:43-7. 2001
    ..We evaluated alternative low-cost methods, by comparing different culture media and storage temperatures...
  36. pmc Nasal swab versus nasopharyngeal aspirate for isolation of respiratory viruses
    Terho Heikkinen
    Department of Pediatrics, Turku University Hospital Department of Virology, University of Turku, Turku, Finland
    J Clin Microbiol 40:4337-9. 2002
    ..The sensitivity of nasal swabs was comparable to that of nasopharyngeal aspirates for the detection of all major respiratory viruses except respiratory syncytial virus...
  37. pmc A model for harmonizing flow cytometry in clinical trials
    Holden T Maecker
    Institute for Immunity, Transplantation and Infection, Stanford University, Stanford, California, USA
    Nat Immunol 11:975-8. 2010
    ..The novel use of a central laboratory may help mitigate these issues...
  38. pmc Development and application of a broadly sensitive dried-blood-spot-based genotyping assay for global surveillance of HIV-1 drug resistance
    Chunfu Yang
    Division of Global AIDS, International Laboratory Branch, DGHA, NCHHSTP, CDC, Mail Stop A 11, 1600 Clifton Road, Atlanta, GA 30333, USA
    J Clin Microbiol 48:3158-64. 2010
    ..Thus, the assay is likely to become a useful screening tool in the global resistance surveillance and monitoring of HIV-1 where multiple subtypes and CRFs are found...
  39. ncbi Evaluation of the ARCHITECT urine NGAL assay: assay performance, specimen handling requirements and biological variability
    Frank C Grenier
    Abbott Laboratories, D 9ML, AP20, 100 Abbott Park Road, Abbott Park, IL 60064, USA
    Clin Biochem 43:615-20. 2010
    ..Reliable clinical evaluations require a simple, robust test method for NGAL, and knowledge of specimen handling and specimen stability characteristics...
  40. ncbi Genotyping whole-genome-amplified DNA from 3- to 25-year-old neonatal dried blood spot samples with reference to fresh genomic DNA
    Mads Vilhelm Hollegaard
    Section of Neonatal Screening and Hormones, Statens Serum Institute, Copenhagen, Denmark
    Electrophoresis 30:2532-5. 2009
    ..Based on these results we conclude that DBS samples should be considered a reliable and potential resource for future genotyping studies...
  41. ncbi Assessment of sample collection and storage methods for multicenter immunologic research in children
    Loren A Matheson
    Department of Pediatrics, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
    J Immunol Methods 339:82-9. 2008
    ..This cost-effective, standardized protocol for a large multicenter trial ensures the integrity of biological samples and maximizes study participation by both large and small centers...
  42. ncbi Evaluation of saliva as a source of human DNA for population and association studies
    Dominique Quinque
    Max Planck Institute for Evolutionary Anthropology, Deutscher Platz 6, D 04103, Leipzig, Germany
    Anal Biochem 353:272-7. 2006
    ..Thus, saliva can be considered a reliable source of DNA for a wide variety of genetic studies...
  43. ncbi Storage policies and use of the Danish Newborn Screening Biobank
    B Nørgaard-Pedersen
    Department of Clinical Biochemistry, Statens Serum Institut, Copenhagen, Denmark
    J Inherit Metab Dis 30:530-6. 2007
    ..The Danish NBS-Biobank has been used in several research projects for aetiological studies of a number of disorders, recently employing new sensitive multiplex technologies and genetic analyses utilizing whole-genome amplified DNA...
  44. ncbi Prevalence and determinants of genital infection with papillomavirus, in female and male university students in Busan, South Korea
    Hai Rim Shin
    Research Institute, National Cancer Center, Goyang si, Gyeonggi Do, Republic of Korea
    J Infect Dis 190:468-76. 2004
    ..Little is known about the prevalence of human papillomavirus (HPV) infection in young adults in Asia...
  45. ncbi Enhanced rates and magnitude of immune responses detected against an HIV vaccine: effect of using an optimized process for isolating PBMC
    Lisa S Kierstead
    Vaccine and Biologics Research, Merck Research Laboratories, West Point and Wayne, PA 19087, USA
    AIDS Res Hum Retroviruses 23:86-92. 2007
    ..These studies establish the importance of optimal conditions for PBMC collection and handling to the success of a clinical development program...
  46. ncbi Incomplete filling of lithium heparin tubes affects the activity of creatine kinase and gamma-glutamyltransferase
    G Lippi
    U O Diagnostica Ematochimica, Azienda Ospedaliero Universitaria di Parma, Italy
    Br J Biomed Sci 69:67-70. 2012
    ..3% and 16.6% for the half-draw and low-draw tubes, respectively. These results suggest that short-draw lithium heparin tubes might be unsuitable for testing GGT and CK using specific combinations of reagents and instrumentation...
  47. ncbi Same day sputum smear microscopy approach for the diagnosis of pulmonary tuberculosis in a microscopy centre at Rajahmundry
    T Jaya Chandra
    Department of Microbiology, GSL Medical College, Rajahmundry, Andhra Pradesh
    Indian J Tuberc 59:141-4. 2012
    ..This requires two (spot and morning SM) sputum sample examinations over two days. Collection of two spot samples (SS2) on the same day would reduce the number of visits, time, money and early initiation of treatment...
  48. ncbi Dry self-sampling versus provider-sampling of cervicovaginal specimens for human papillomavirus detection in the Inuit population of Nunavik, Quebec
    Helen Cerigo
    Department of Epidemiology, Biostatistics and Occupational Health, McGill University, Montreal, Canada
    J Med Screen 19:42-8. 2012
    ....
  49. ncbi Measuring the incidence, prevalence and genetic relatedness of hepatitis C infections among a community recruited sample of injecting drug users, using dried blood spots
    V D Hope
    Centre for Infections, Health Protection Agency, London, UK
    J Viral Hepat 18:262-70. 2011
    ..They also enable virus characterization at genotype and nucleotide level. This would allow surveillance to inform development of harm reduction interventions, and the international evidence base for these...
  50. ncbi An episode of increased hemolysis due to a defective pneumatic air tube delivery system
    Graham Ellis
    Department of Clinical Biochemistry, St John s Hospital, Livingston West Lothian, Scotland, UK
    Clin Biochem 42:1265-9. 2009
    ..To study an episode of excessive sample hemolysis associated with a recently installed pneumatic tube system from the Accident and Emergency Department...
  51. ncbi Increased sensitivity of malaria detection by nested polymerase chain reaction using simple sampling and DNA extraction
    J Cox-Singh
    School of Medical Sciences, University of Science Malaysia, Kelantan, Malaysia
    Int J Parasitol 27:1575-7. 1997
    ....
  52. ncbi Long-term storage of DNA-free RNA for use in vaccine studies
    Kathryn L Jones
    Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Australia
    Biotechniques 43:675-81. 2007
    ..g., 4 degrees C) can be achieved...
  53. ncbi Assessment of sample handling practices on microbial activity in sputum samples from patients with cystic fibrosis
    A Nelson
    School of Applied Sciences, Ellison Building, University of Northumbria, Newcastle upon Tyne, UK
    Lett Appl Microbiol 51:272-7. 2010
    ..The aim of this study was to quantitatively and qualitatively assess the effect of sample storage on the metabolically active microbial community found in sputum samples from patients with cystic fibrosis (CF)...
  54. pmc Identification of blood culture isolates directly from positive blood cultures by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry and a commercial extraction system: analysis of performance, cost, and turnaround time
    Philippe R S Lagacé-Wiens
    Department of Medical Microbiology and Infectious Diseases, University of Manitoba Winnipeg, Manitoba, Canada
    J Clin Microbiol 50:3324-8. 2012
    ..However, the use of MALDI-TOF for direct identification is accurate and should result in reduced turnaround time to identification...
  55. ncbi Standard operating procedures for pre-analytical handling of blood and urine for metabolomic studies and biobanks
    Patrizia Bernini
    Magnetic Resonance Center CERM, University of Florence, Via L Sacconi 6, 50019, Sesto Fiorentino, Italy
    J Biomol NMR 49:231-43. 2011
    ..Appropriate procedures for blood derivatives collection and urine preservation/storage that allow maintaining as much as possible the original metabolic profile of the fresh samples emerge, and are proposed as SOPs for biobanking...
  56. ncbi Preanalytical quality control program - an overview of results (2001-2005 summary)
    Ma Jesus Alsina
    SEQC Committee for the Quality of the Extraanalytical Phase, Spain
    Clin Chem Lab Med 46:849-54. 2008
    ..Preanalytical variables, such as sample collection, handling, transport, and storage, may affect patient results. The number of errors in the preanalytical phase may decrease by following standardized procedures...
  57. ncbi Detection of Puumala and Rift Valley Fever virus by quantitative RT-PCR and virus viability tests in samples of blood dried and stored on filter paper
    Jonas Näslund
    Department of Clinical Microbiology, Division of Infectious Diseases, Umea University, SE 901 85 Umea, Sweden
    J Virol Methods 178:186-90. 2011
    ..This approach might facilitate sampling and analysis of other RNA viruses from human or animal sources and could be used for field studies in remote areas or in developing countries...
  58. pmc Phosphoprotein stability in clinical tissue and its relevance for reverse phase protein microarray technology
    Virginia Espina
    Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, VA, USA
    Methods Mol Biol 785:23-43. 2011
    ..Based on this analysis we have established tissue procurement guidelines for clinical research with an emphasis on quantifying phosphoproteins by RPMA...
  59. ncbi Lymph node harvest in colon cancer specimens depends on tumour factors, patients and doctors, but foremost on specimen handling
    Kristian Storli
    Department of Surgery, Haraldsplass Deaconal Hospital, Bergen, Norway
    APMIS 119:127-34. 2011
    ..Several tumour and individual patient characteristics, surgical approach and specimen handling may influence lymph node yield and theoretically, TNM staging...
  60. ncbi Agreement between paired blood gas values in samples transported either by a pneumatic system or by human courier
    John Victor Peter
    Medical ICU, Christian Medical College and Hospital, Vellore, India
    Clin Chem Lab Med 49:1303-9. 2011
    ..Rapid accurate assessment of metabolic derangements is crucial in the critically ill. We evaluated if arterial blood gas (ABG) samples transported through a pneumatic tube system (PTS) agreed with values transported by a human courier...
  61. ncbi Volatile organic compounds from feces and their potential for diagnosis of gastrointestinal disease
    Catherine E Garner
    Clinical Science at South Bristol, Bristol Royal Infirmary, Marlborough St, Bristol BS2 8HW, UK
    FASEB J 21:1675-88. 2007
    ..difficile, and C. jejuni were each significantly different. These findings could lead the way to the development of a rapid diagnostic device based on VOC detection...
  62. pmc Storage and stability of IgG and IgM monoclonal antibodies dried on filter paper and utility in Neisseria meningitidis serotyping by Dot-blot ELISA
    Aline S Ferraz
    Immunology Section, Adolfo Lutz Institute, Sao Paulo, Sao Paulo, Brazil
    BMC Infect Dis 8:30. 2008
    ..meningitidis by dot-blot ELISA...
  63. pmc Managing the pre- and post-analytical phases of the total testing process
    Robert Hawkins
    Department of Laboratory Medicine, Tan Tock Seng Hospital, Tan Tok Seng, Singapore
    Ann Lab Med 32:5-16. 2012
    ....
  64. pmc Assessing a novel room-temperature RNA storage medium for compatibility in microarray gene expression analysis
    Gilberto E Hernandez
    DNA Array Core Facility, The Scripps Research Institute, La Jolla, California 92037, USA
    Biotechniques 47:667-8, 670. 2009
    ..The ability to stabilize RNA samples at ambient temperatures for extended time periods will have tremendous use, particularly for sample shipment to core facilities...
  65. ncbi Solution densities and estimated total protein contents associated with inappropriate flotation of separator gel in different blood collection tubes
    Ronald C Faught
    Clinical Laboratory, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    Arch Pathol Lab Med 135:1081-4. 2011
    ..In automated analytical systems, gel flotation can lead to mechanical disruption and potential inaccurate result reporting upon aspiration into instrument sampling probes...
  66. ncbi Development of chip-compatible sample preparation for diagnosis of infectious diseases
    Marion Ritzi-Lehnert
    Institut fuer Mikrotechnik Mainz GmbH, Mainz, Germany
    Expert Rev Mol Diagn 12:189-206. 2012
    ..In this article, the most recent advances in on-chip sample preparation are reviewed for microfluidic-based diagnosis of infectious diseases...
  67. ncbi The challenge and importance of standardizing pre-analytical variables in surgical pathology specimens for clinical care and translational research
    D G Hicks
    Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, 601 Elmwood Avenue, Box 626, Rochester, NY 14642, USA
    Biotech Histochem 87:14-7. 2012
    ..Inaccurate results owing to compromised tissue quality can lead to false positive or false negative results with therapeutic consequences that can harm patients and affect their eventual outcome...
  68. ncbi Organizational issues in providing high-quality human tissues and clinical information for the support of biomedical research
    Walter C Bell
    Department of Pathology, University of Alabama at Birmingham, Birmingham, AL, USA
    Methods Mol Biol 576:1-30. 2010
    ..In this review, we describe, based on our experience, the major issues that should be addressed in designing and establishing a tissue repository...
  69. ncbi Detection and species identification of Leishmania DNA from filter paper lesion impressions for patients with American cutaneous leishmaniasis
    Andrea K Boggild
    Tropical Disease Unit, Division of Infectious Diseases, Toronto General Hospital, Toronto, Canada
    Clin Infect Dis 50:e1-6. 2010
    ..We compared traditional diagnostic methods with a molecular noninvasive filter paper-based method for the diagnosis of cutaneous leishmaniasis...
  70. ncbi Quality management for the collection of biological samples in multicentre studies
    J Peplies
    Bremen Institute for Prevention Research and Social Medicine, University of Bremen, Germany
    Eur J Epidemiol 25:607-17. 2010
    ..Overall compliance with the QMS-BS was good, with some exceptions mainly related to sample control. In conclusion the QMS-BS is a valuable tool for the management of biological sample collection in epidemiological multicentre studies...
  71. ncbi Early diagnosis of human immunodeficiency virus-1 infection in infants with the NucliSens EasyQ assay on dried blood spots
    R R Lilian
    Paediatric HIV Diagnostic Syndicate, Wits Health Consortium, 8 Blackwood Avenue, Parktown, Johannesburg, South Africa
    J Clin Virol 48:40-3. 2010
    ..The VL assay routinely used in the country has not previously been assessed on DBS for early infant diagnosis...
  72. pmc Does bleach processing increase the accuracy of sputum smear microscopy for diagnosing pulmonary tuberculosis?
    A Cattamanchi
    Division of Pulmonary and Critical Care Medicine, San Francisco General Hospital, University of California, San Francisco, CA 94110, USA
    J Clin Microbiol 48:2433-9. 2010
    ..Further research should focus on alternative approaches to optimizing smear microscopy, such as light-emitting diode fluorescence microscopy and same-day sputum collection strategies...
  73. ncbi Prevalence of type-specific human papillomavirus in endocervical, upper and lower vaginal, perineal and vaginal self-collected specimens: Implications for vaginal self-collection
    Jerome L Belinson
    Department of Gynecology, The Cleveland Clinic Foundation, Cleveland, OH, USA
    Int J Cancer 127:1151-7. 2010
    ..The principal cause of the lower specificity of self-collected specimens is HR-HPV present solely in the vagina, which is not associated with > or = CIN 2...
  74. pmc Radiological and pathological size estimations of pure ductal carcinoma in situ of the breast, specimen handling and the influence on the success of breast conservation surgery: a review of 2564 cases from the Sloane Project
    J Thomas
    Pathology Department, Western General Hospital and Breakthrough Breast Cancer Research Unit, Edinburgh EH4 2XU, UK
    Br J Cancer 102:285-93. 2010
    ..We have correlated these sizes with histological grade, specimen-handling methods, particularly the use of specimen slice radiographs, and the success or failure of breast-conserving surgery (BCS)...
  75. ncbi Dried blood spot HIV-1 RNA quantification using open real-time systems in South Africa and Burkina Faso
    Johannes Viljoen
    Africa Center for Health and Population Studies, University of KwaZulu Natal, Durban, South Africa
    J Acquir Immune Defic Syndr 55:290-8. 2010
    ..Our findings strongly advocate the implementation of DBS HIV-1 RNA testing in remote areas from low-income and middle-income countries...
  76. ncbi HPV DNA testing of self-collected vaginal samples compared with cytologic screening to detect cervical cancer
    T C Wright
    Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    JAMA 283:81-6. 2000
    ..In many other regions of the world, there is only limited access to cervical cancer screening...
  77. ncbi To continue or discontinue storage of cryopreserved embryos? Patients' decisions in view of their child wish
    V Provoost
    Bioethics Institute Ghent, Ghent University, Blandijnberg 2, B 9000 Ghent, Belgium
    Hum Reprod 26:861-72. 2011
    ....
  78. pmc Pooling strategies to reduce the cost of HIV-1 RNA load monitoring in a resource-limited setting
    G U van Zyl
    Division of Medical Virology, Stellenbosch University, and National Health Laboratory Service, Tygerberg, South Africa
    Clin Infect Dis 52:264-70. 2011
    ..Recently, the use of pooling strategies with a clinically appropriate viral load threshold was shown to be accurate and efficient for monitoring when the prevalence of virologic failure is low...
  79. ncbi The use of FTA® filter papers for diagnosis of avian influenza virus
    E M Abdelwhab
    Institute of Poultry Diseases, Free Berlin University, Koenigsweg 63, 14163 Berlin, Germany
    J Virol Methods 174:120-2. 2011
    ..The use of FTA® cards will facilitate safe transport of samples for molecular diagnosis of AIV avoiding the need for an uninterrupted cold storage...
  80. ncbi Improved sensitivity of vaginal self-collection and high-risk human papillomavirus testing
    Jerome L Belinson
    Preventive Oncology International, Inc Cleveland Heights, OH, USA
    Int J Cancer 130:1855-60. 2012
    ..Large population-based screening "events" in low-resource settings could be achieved by promoting self-collection and centralized high-throughput, low-cost testing by PCR-based MALDI-TOF...
  81. ncbi E-mail-based symptomatic surveillance combined with self-collection of nasal swabs: a new tool for acute respiratory infection epidemiology
    Manas K Akmatov
    Department of Infection Genetics, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany
    Int J Infect Dis 15:e799-803. 2011
    ..We examined the feasibility of combining communication by e-mail and self-collection of nasal swabs for the prospective detection of acute respiratory infections in a non-medical setting...
  82. ncbi Quality improvement in laboratory medicine: extra-analytical issues
    Giuseppe Lippi
    Sezione di Chimica e Microscopia Clinica, Dipartimento di Scienze Morfologico Biomediche, Universita degli Studi di Verona, Ospedale Policlinico GB Rossi, Piazzale Scuro, Verona, Italy
    Clin Lab Med 28:285-94, vii. 2008
    ..The article focuses on appropriateness of the test request, patient and sample identification, criteria for acceptance and rejection of specimens, and the communication and interpretation of results...
  83. ncbi EGFR and KRAS mutations detection on lung cancer liquid-based cytology: a pilot study
    Umberto Malapelle
    Scienze Biomorfologiche e Funzionali, University Federico II of Naples, Naples, Italy
    J Clin Pathol 65:87-91. 2012
    ..Although time-consuming, LCM makes direct sequencing highly sensitive even on LBC preparations containing only a few cells...
  84. ncbi Evaluation of a sticky trap for collecting Aedes (Stegomyia) adults in a dengue-endemic area in Thailand
    Luca Facchinelli
    Parasitology Unit, Department of Public Health Sciences, University of Rome La Sapienza, Rome, Italy
    Am J Trop Med Hyg 78:904-9. 2008
    ..The percentage of positive sticky-trap catches was double for Ae. aegypti and almost 20 times higher for Ae. albopictus. Operational benefits of the sticky trap are discussed within the context of the results obtained...
  85. pmc Comparative performance of human papillomavirus DNA testing using novel sample collection methods
    Julia C Gage
    Clinical Genetics Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, 6120 Executive Blvd, MSC 7231, Rockville, MD 20852, USA
    J Clin Microbiol 49:4185-9. 2011
    ..HC2 is likely more clinically specific, although possibly less sensitive, than either PCR test. Thus, use of HC2 on cervicovaginal specimens for screening could result in fewer referrals compared to LA and Amplicor...
  86. ncbi Tuberculosis in children: reassessing the need for improved diagnosis in global control strategies
    P Eamranond
    Centro Internacional de Entrenamiento e Investigaciones Medicas, Cali, Colombia
    Int J Tuberc Lung Dis 5:594-603. 2001
    ..In this review, we present compelling evidence that research agendas and TB control programs should be reassessed and possibly revised to deal with the global disease caused by PPTB...
  87. ncbi Self-sampling of vaginal fluid and high-risk human papillomavirus testing in women aged 50 years or older not attending Papanicolaou smear screening
    M Lindell
    Department of Pathology and Cytology, Uppsala University Hospital, Sweden
    BJOG 119:245-8. 2012
    ..To study the value of self-sampling of vaginal fluid at home in combination with high-risk human papillomavirus (HPV) testing in a cohort of older women not attending Papanicolaou (Pap) smear screening...
  88. pmc Using rapid diagnostic tests as source of malaria parasite DNA for molecular analyses in the era of declining malaria prevalence
    Deus S Ishengoma
    National Institute for Medical Research, Tanga Centre, Bombo Road, P, O Box 5004, Tanga, Tanzania
    Malar J 10:6. 2011
    ....
  89. ncbi Best practice in forensic entomology--standards and guidelines
    Jens Amendt
    Institute of Forensic Medicine, University of Frankfurt, Kennedyallee 104, 60596, Frankfurt Main, Germany
    Int J Legal Med 121:90-104. 2007
    ....
  90. ncbi Impact of intensified testing for urogenital Chlamydia trachomatis infections: a randomised study with 9-year follow-up
    Berit Andersen
    Department of Public Health Interventions, Randers Hospital, Skovlyvej, 8900 Randers, Denmark
    Sex Transm Infect 87:156-61. 2011
    ..The long-term clinical benefit in terms of avoided infertility and ectopic pregnancy is unknown...
  91. ncbi International Society of Urological Pathology (ISUP) Consensus Conference on Handling and Staging of Radical Prostatectomy Specimens. Working group 1: specimen handling
    Hemamali Samaratunga
    Department of Pathology, Aquesta Pathology, Brisbane, QLD, Australia
    Mod Pathol 24:6-15. 2011
    ....
  92. ncbi Diagnosis of pulmonary tuberculosis in children: new advances
    Heather J Zar
    Department of Paediatrics and Child Health, Red Cross War Memorial Children s Hospital, University of Cape Town, South Africa
    Expert Rev Anti Infect Ther 8:277-88. 2010
    ..Greater efforts to obtain a microbiologic diagnosis should be made in children, even in primary care settings. Further research to develop a more accurate, cost-effective and simple diagnostic test for childhood TB is urgently needed...
  93. pmc Defining blood processing parameters for optimal detection of cryopreserved antigen-specific responses for HIV vaccine trials
    Marta Bull
    Children s Hospital and Regional Medical Center, Seattle, WA, USA
    J Immunol Methods 322:57-69. 2007
    ..This narrow window for specimen processing has important implications in selecting and monitoring clinical sites with laboratory capacity to perform these procedures in future clinical trials...
  94. pmc Optimization and limitations of use of cryopreserved peripheral blood mononuclear cells for functional and phenotypic T-cell characterization
    Adriana Weinberg
    University of Colorado Denver School of Medicine, Denver, CO, USA
    Clin Vaccine Immunol 16:1176-86. 2009
    ....
  95. ncbi Stability of dried blood spots for HIV-1 drug resistance analysis
    Anna C Hearps
    Centre for Virology, Burnet Institute, 85 Commercial Road, Melbourne 3004, Australia
    Curr HIV Res 8:134-40. 2010
    ....
  96. ncbi Effect of sampling time on the culturability of airborne fungi and bacteria sampled by filtration
    Kate T H Durand
    University of Washington, School of Public Health and Community Medicine, Department of Environmental Health, Seattle 98195, USA
    Ann Occup Hyg 46:113-8. 2002
    ..This suggests that increased sampling time does not affect the viability of the organisms commonly found in the air at composting facilities...
  97. ncbi A GC-TOF-MS study of the stability of serum and urine metabolomes during the UK Biobank sample collection and preparation protocols
    Warwick B Dunn
    Bioanalytical Sciences Group, School of Chemistry, Manchester Interdiscplinary Biocentre, University of Manchester, 131 Princess Street, Manchester, M1 7ND, UK
    Int J Epidemiol 37:i23-30. 2008
    ....
  98. ncbi Off-line exhaled nitric oxide measurements in children
    M Barreto
    II Pediatric Department, University La Sapienza, Rome, Italy
    Pediatr Pulmonol 32:159-67. 2001
    ..Once standardized, the off-line eNO technique using 1-L gas collection bags will provide results similar to those recorded on-line...
  99. ncbi Optimizing buccal cell DNA yields in mothers and infants for human leukocyte antigen genotyping
    Audrey F Saftlas
    Department of Epidemiology, University of Iowa College of Public Health, Iowa City, IA 52242, USA
    Am J Epidemiol 160:77-84. 2004
    ..Cytobrush sampling of the mouth's gutter areas can enhance DNA yield in mothers but not in young infants. DNA yields can be further optimized by controlling mailing and storage conditions...
  100. pmc Detection of carcinogenic human papillomavirus in specimens collected with a novel self-sampling device
    Philip E Castle
    Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD 20892 7234, USA
    J Clin Microbiol 44:2158-9. 2006
    ..The kappa value was 0.66 (95% confidence interval, 0.53 to 0.78), with an 83% overall test agreement and a 68% positive test agreement...
  101. ncbi Classifying laboratory incident reports to identify problems that jeopardize patient safety
    Michael L Astion
    Department of Laboratory Medicine, University of Washington School of Medicine, Seattle, WA, USA
    Am J Clin Pathol 120:18-26. 2003
    ....

Research Grants63

  1. Novel Method for Sample Processing
    Yajuan Jiang; Fiscal Year: 2006
    ..g. fine needle aspirates or other biopsy materials). Problems associated with specimen handling include cell loss and cell clumping...
  2. Comprehensive Molecular Diagnosis for Food and Waterborne Enteropathogens
    Eric R Houpt; Fiscal Year: 2011
    ..is to develop sensitive PCR-based diagnostics directly usable on stool with comparable or better sensitivity to existing technologies, which have clear deficincies in sensitivity of pathogen detection, throughput, and specimen handling.
  3. Automated point-of-care PCR for hemorrhagic fever diagnosis
    Alan L Rothman; Fiscal Year: 2010
    ..of a proprietary assay tube system and analyzer device, provides a closed system for rapid, automated specimen handling and nucleic acid detection...
  4. DEVELOPMENT OF A SALIVA HOMOCYSTEINE ASSAY SYSTEM
    Timothy Carlson; Fiscal Year: 1999
    ..The elaborate specimen handling necessary to prevent this is frequently not achievable in actual practice...
  5. Macromolecular Crystallography at the National Synchrotron Light Source
    Robert M Sweet; Fiscal Year: 2011
    ..collimation of modern x-ray sources, the use of micron-sized crystals, the development of robotic cryogenic specimen handling, and the simultaneous measurement of UV, Visible, or Raman IR spectra with x-ray diffraction...
  6. Macromolecular Crystallography at the National Synchrotron Light Source
    Robert M Sweet; Fiscal Year: 2013
    ..and collimation of modern x-ray sources, use of micron-sized crystals, development of robotic cryogenic specimen handling, and simultaneous measurement of UV, Visible, or Raman IR spectra with x-ray diffraction...
  7. HEMOQUANT DETECTION OF COLORECTAL NEOPLASIA
    David Ahlquist; Fiscal Year: 1990
    ..central office coordinate all subsequent operations including patient and physician correspondence, specimen handling, and data management. All fecal blood assays are done at Mayo laboratories...
  8. QUANTITATIVE MEASURES IN BREAST CANCER PATHOLOGY
    Martha Hutchinson; Fiscal Year: 1990
    ..However, until now the necessity for special specimen handling, laborious methods, and/or expensive computers for quantitative cell image analysis have limited the ..
  9. NIAID Specimen Repository
    MARK MANAK; Fiscal Year: 2010
    ..Provide shipping materials and documents, technical assistance and training to Site personnel and study teams on specimen handling and shipping
  10. NIAID Specimen Repository
    Mark M Manak; Fiscal Year: 2009
    ..Provide shipping materials and documents, technical assistance and training to Site personnel and study teams on specimen handling and shipping
  11. NIAID Specimen Repository
    Michael Waddington; Fiscal Year: 2011
    ..Provide shipping materials and documents, technical assistance and training to Site personnel and study teams on specimen handling and shipping
  12. Wide Angle X-ray Scattering for Commercial Applications in Drug Lead Discovery
    Richard Walter; Fiscal Year: 2009
    ..and associated control systems by constructing a portable, wide-angle camera with robotic control of specimen handling;(2) to develop a software package to automate downstream data processing and analysis by updating and ..
  13. INTELLIGENT REFRIGERATED AUTOMATIC STORAGE SYSTEM
    STEPHEN RENSHAW; Fiscal Year: 1991
    ..3. Decreasing the risk of infection of laboratory personnel by decreasing the amount of manual specimen handling. Phase I includes an evaluation of both the hardware and software prior to designing a sophisticated Phase ..
  14. Purchase of a FEI Titan Krios for 3-D EM
    Kenneth Taylor; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  15. Effect of Oxidant Load on HPV Persistence and CIN (SIL)
    Anna Giuliano; Fiscal Year: 2008
    ..abstract_text> ..
  16. Urinary Symptoms in Adolescent Females: STI or UTI?
    Jill Huppert; Fiscal Year: 2004
    ..The findings of this study will strengthen the evidence base for symptom-based STI testing of SA adolescent females and will promote care that addresses both individual patient and public health needs. ..
  17. CryoEM Equipment Enhancements for Florida State University
    Kenneth Taylor; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  18. Liquid Chromatography Mass Spectrometry system
    Adrian Franke; Fiscal Year: 2005
    ..abstract_text> ..
  19. Purchase of a large format CCD camera for 3-D EM
    Kenneth Taylor; Fiscal Year: 2005
    ..The new instrumentation will allow for development of algorithms for increasing the area that can be reconstructed by electron tomography which may need corrections for focus gradient and for imaging distortions. ..
  20. Predicting Metastasis to Lymph Nodes
    David Rimm; Fiscal Year: 2008
    ....
  21. Ectopic Pregnancy: Assessing Long-Term Trends in Rates and Risk Factors
    Delia Scholes; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  22. Multiplexed pathogen detection by on-chip amplification
    Darrell Chandler; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  23. Determination of OA Pathology by Biomarker Dating
    Virginia Kraus; Fiscal Year: 2007
    ..We refer to the technique of quantifying the D-aspartate content of cartilage macromolecules as "biomarker dating". ..
  24. Neoadjuvant Cytokine Immunotherapy by PLA Microspheres
    Michael Sabel; Fiscal Year: 2007
    ..I will pursue this area of investigation as a full-time faculty member, with full commitment from the Department of General Surgery and the Division of Surgical Oncology at the University of Michigan Medical Center. ..
  25. Diagnostic protein array for respiratory infections
    Darrell Chandler; Fiscal Year: 2008
    ..The underlying platform developed herein will likewise find broad application in many areas of disease surveillance, with an emphasis on point-of-use applications. [unreadable] [unreadable] [unreadable] [unreadable] [unreadable]..
  26. Pregnancy-Related Sleep Disturbances May Increase Pregnancy Complications
    Michele Okun; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  27. MALIGNANT COMPLICATIONS OF CHRONIC HCV
    Christopher Loffredo; Fiscal Year: 2009
    ..This set of aims and molecular epidemiology methods represents a novel approach to the study of the malignant complications of chronic HCV infection. ..
  28. Early experience and low 5-HT markers in alcohol abuse
    Mark Laudenslager; Fiscal Year: 2009
    ..Finally, involvement of the HPA axis in these relationships will be investigated as an exploratory goal. ..
  29. Gender Differences in Bladder Cancer Risk Factors
    Christopher A Loffredo; Fiscal Year: 2010
    ..squamous). The results of this study will help establish the best preventive measures for bladder cancer in men and women, by appropriately directing the resources of the public health sector for effective cancer control. ..
  30. Effects of Soy on Estrogens in Breast Fluid and Urine
    Gertraud Maskarinec; Fiscal Year: 2010
    ..The NAF approach is the only way to learn more about the beneficial and adverse effects of soy directly in the female breast without applying invasive procedures to women without breast disease or surgery. ..
  31. LIPID METABOLISM IN FAT CELLS
    Wen Guo; Fiscal Year: 2010
    ..Together, these studies will provide new insights into the stress-induced regulation of lipogenesis in adipocytes, a potentially important avenue for modulating energy balance. ..
  32. The ethics of consent for the public release of potentially identifiable DNA data
    Amy McGuire; Fiscal Year: 2009
    ..Moreover, it will increase our understanding of participants'judgments and attitudes toward data sharing, which will improve the overall conduct of biomedical research, build public trust, and foster research participation. ..
  33. GENETIC MECHANISMS THAT POTENTIATE HUMAN SPERMATOGENESIS
    STEPHEN KRAWETZ; Fiscal Year: 2009
    ..This milestone provides the foundation to isolate and functionally characterize the associated agents that open chromatin domains. ..
  34. Novel Application of Infection Control Strategies to Limit Transmission of ESBL'S
    Ebbing Lautenbach; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  35. Utilizing Saliva to Measure Bone Turnover
    Sreenivas Koka; Fiscal Year: 2007
    ..We anticipate that our results will identify how salivary biomarkers can be used to assess the osseo-status of patients with, or at risk for, osteoporosis. [unreadable] [unreadable] [unreadable] [unreadable]..
  36. CCBAR Workshop 2006-2007
    STACY LINDAU; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  37. New Tool to Study Neurochemical Modulation of Feeding
    SCOTT SHIPPY; Fiscal Year: 2005
    ..The unique capabilities of low-flow push-pull perfusion complement other sampling methodologies and are widely applicable to in vivo sampling studies. ..
  38. ED Screening and Interventions for Hazardous Alcohol Use
    Brigitte Baumann; Fiscal Year: 2004
    ..The intent of this proposal is to join with 12 other participating EDs in a joint effort of SBIRT training and ED patient intervention. ..
  39. Automation Technology for High Throughput Cryo-EM
    Robert Glaeser; Fiscal Year: 2004
    ..High throughput determination of the structure of large macromolecular assemblies will greatly enhance our understanding of the ways in which complex biochemical operations are carried out at the subcellular level. ..
  40. The Effect of Black Cohosh Extract on the Human Breast
    Edward Sauter; Fiscal Year: 2004
    ..The findings should allow us to design a high quality R01 submission involving a larger, randomized study of longer duration which should provide some answers on the potential role of BCE in breast cancer. ..
  41. HIV-1 resistance to nucleoside analogues
    Daniel Kuritzkes; Fiscal Year: 2004
    ..Better understanding of the virologic, genetic, and biochemical aspects of AZT/3TC dual resistance will help direct therapy and may lead to better therapeutic strategies in the future. ..
  42. AQUA: A New Quantitative in situ Proteomic Assay
    David Rimm; Fiscal Year: 2004
    ....
  43. THE CLOUD ADULT
    Robert Sherertz; Fiscal Year: 2003
    ..aureus associated with a viral URI, have been shown to cause outbreaks. Understanding the "cloud phenomenon" may facilitate S.aureus outbreak prevention and possibly the transmission of other pathogens, as well. ..
  44. HUMAN RETROVIRUS-5 AND ARTHRITIS
    Robin Patel; Fiscal Year: 2002
    ..Identification of a specific infectious agent associated with these arthritides would potentially allo for the development of preventive strategies such as vaccination and novel therapeutic approaches. ..
  45. EPITHELIAL GENE TRANSCRIPTION IN INTESTINAL PATHOLOGY
    Gary Wu; Fiscal Year: 2002
    ..Ultimately, once the mechanisms by which DRA gene transcription is regulated in the normal colon have been elucidated, DRA may be a useful model with which to investigate how gene expression is altered in pathobiology. ..
  46. ESTABLISHMENT AND REGULATION OF CELL/CELL ADHESION
    David Rimm; Fiscal Year: 2002
    ..We use this tool throughout this proposal in in vitro adhesion assays and also as a unique probe for the homotypic interaction, as a mechanism to assess the co-precipitable components in the adhesion complex. ..
  47. NESTED CASE CONTROL STUDY OF MAMMOGRAPHIC DENSITY
    Gertraud Maskarinec; Fiscal Year: 2002
    ....
  48. Altered Breast Methylation after Low/Hi Dose Isoflavones
    Edward Sauter; Fiscal Year: 2005
    ..abstract_text> ..
  49. Enhancement of antimicrobics by electrical current
    Robin Patel; Fiscal Year: 2007
    ..This novel but simple resistance reversing strategy may generalize to a wide variety of antimicrobics and microorganisms and a wide variety of biofilm-associated human infections. [unreadable] [unreadable] [unreadable] [unreadable]..
  50. Equipment for Correlative LM/EM by High Pressure Freezing and Tomography
    Kent McDonald; Fiscal Year: 2007
    ..In turn, this better understanding of mechanism will promote more effective treatments for disease intervention. [unreadable] [unreadable] [unreadable]..
  51. Human papillomavirus as a risk factor for HIV infection
    PETER CHIN HONG; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  52. Soy Consumption and Breast Tissue Proliferation
    Gertraud Maskarinec; Fiscal Year: 2006
    ....
  53. GENOME RESOURCE BANKING OF RARE FELID POPULATIONS
    William Swanson; Fiscal Year: 2006
    ..abstract_text> ..
  54. Egypt Smoking Prevention Research Initiative
    Christopher Loffredo; Fiscal Year: 2006
    ..The establishment of the ESPRI and the professional development of Egyptian investigators and Research Associates will accelerate Egypt's efforts to become tobacco free. ..
  55. PCR assays to detect individual pneumococcal serotypes
    LORRY RUBIN; Fiscal Year: 2006
    ..Furthermore, they will be critical for determining the effects of current and future pneumococcal vaccines on serotype-specific carriage. ..
  56. Fitness of Enfuvirtide-(T-20)-Resistant HIV-1
    Daniel Kuritzkes; Fiscal Year: 2006
    ..Results of these studies will provide a deeper understanding of the molecular, virologic, and clinical consequences ofT-20 resistance, and may help guide the use of T-20 in salvage therapy. ..
  57. Functional Assessment of Pulmonary Toxicity with MRM
    G Johnson; Fiscal Year: 2005
    ..abstract_text> ..
  58. From drug Use to Addiction: Unearthing the Switches
    Howard Gutstein; Fiscal Year: 2005
    ..It is hoped that these findings will eventually lead to improved treatments for these devastating conditions. ..
  59. CLINICAL RESEARCH CURRICULUM
    David Ransohoff; Fiscal Year: 2004
    ..abstract_text> ..
  60. Colonoscopy Utilization in North Carolina
    David Ransohoff; Fiscal Year: 2006
    ..It may provide the basis to develop interventions to optimize the allocation of colonoscopy effort in programs of CRC screening and surveillance. ..
  61. LEICA EM UC/6 CRYO-ULTRAMICROTOME AND ACCESSORIES
    Kent McDonald; Fiscal Year: 2005
    ..Use by regional investigators will be encouraged in the time remaining after core investigator use. UC Berkeley will provide space, staff, and funds for maintaining the equipment. ..