fluorescence polarization immunoassay

Summary

Summary: Fluoroimmunoassay where detection of the hapten-antibody reaction is based on measurement of the increased polarization of fluorescence-labeled hapten when it is combined with antibody. The assay is very useful for the measurement of small haptenic antigens such as drugs at low concentrations.

Top Publications

  1. ncbi Serologic detection of experimental Salmonella enteritidis infections in laying hens by fluorescence polarization and enzyme immunoassay
    Richard K Gast
    United States Department of Agriculture, Agricultural Research Service, Southeast Poultry Research Laboratory, Athens, GA 30605, USA
    Avian Dis 46:137-42. 2002
  2. ncbi A new enzyme-linked chemiluminescent immunosorbent digoxin assay is virtually free from interference of spironolactone, potassium canrenoate, and their common metabolite canrenone
    Amitava Dasgupta
    Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Clin Lab Anal 20:204-8. 2006
  3. ncbi [Analysis of detecting methods of digoxin blood drug level]
    You Xin Li
    Pharmacy College, Tianjin Medical University, Tianjin 300070, China
    Zhongguo Zhong Yao Za Zhi 32:285-8, 326. 2007
  4. ncbi A simple, rapid and high-throughput fluorescence polarization immunoassay for simultaneous detection of organophosphorus pesticides in vegetable and environmental water samples
    Zhen Lin Xu
    Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China
    Anal Chim Acta 708:123-9. 2011
  5. ncbi Assay of teicoplanin in serum: comparison of high-performance liquid chromatography and fluorescence polarization immunoassay
    Steven J McCann
    Department of Clinical Biochemistry, Wythenshawe Hospital, Southmoor Road, Manchester M23 9LT, UK
    J Antimicrob Chemother 50:107-10. 2002
  6. ncbi Application of fluorescence polarization immunoassay for determination of methotrexate-polyglutamates in rheumatoid arthritis patients
    Hideki Hayashi
    Department of Clinical Pharmacology and Genetics, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan
    Tohoku J Exp Med 215:95-101. 2008
  7. ncbi The application of CdTe/CdS in the detection of carcinoembryonic antigen by fluorescence polarization immunoassay
    Jianniao Tian
    Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources Ministry of Education of China, College of Chemistry and Chemical Engineering of Guangxi Normal University, Guilin, China
    J Fluoresc 22:1571-9. 2012
  8. ncbi Toxicological detection of the designer drug 3,4-methylenedioxyethylamphetamine (MDE, "Eve") and its metabolites in urine by gas chromatography-mass spectrometry and fluorescence polarization immunoassay
    H K Ensslin
    Pharmaceutical Institute, University of Tubingen, Germany
    J Chromatogr B Biomed Appl 683:189-97. 1996
  9. ncbi Multiplexed detection of tumor markers with multicolor quantum dots based on fluorescence polarization immunoassay
    Jianniao Tian
    Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources Ministry of Education of China, College of Chemistry and Chemical Engineering of Guangxi Normal University, Guilin 541004, China
    Talanta 92:72-7. 2012
  10. ncbi A rapid fluorescence polarization immunoassay for the determination of T-2 and HT-2 toxins in wheat
    Vincenzo Lippolis
    Institute of Sciences of Food Production ISPA, CNR National Research Council of Italy, Via G Amendola 122 o, 70126 Bari, Italy
    Anal Bioanal Chem 401:2561-71. 2011

Detail Information

Publications226 found, 100 shown here

  1. ncbi Serologic detection of experimental Salmonella enteritidis infections in laying hens by fluorescence polarization and enzyme immunoassay
    Richard K Gast
    United States Department of Agriculture, Agricultural Research Service, Southeast Poultry Research Laboratory, Athens, GA 30605, USA
    Avian Dis 46:137-42. 2002
    ..typhimurium significantly less often than the ELISA. The fluorescence polarization assay also offered advantages in terms of speed and methodologic simplicity...
  2. ncbi A new enzyme-linked chemiluminescent immunosorbent digoxin assay is virtually free from interference of spironolactone, potassium canrenoate, and their common metabolite canrenone
    Amitava Dasgupta
    Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Clin Lab Anal 20:204-8. 2006
    ..of spironolactone and related compounds in this assay by comparing the results with the fluorescence polarization immunoassay (FPIA), which is known to have significant cross-reactivity with these compounds as well as a ..
  3. ncbi [Analysis of detecting methods of digoxin blood drug level]
    You Xin Li
    Pharmacy College, Tianjin Medical University, Tianjin 300070, China
    Zhongguo Zhong Yao Za Zhi 32:285-8, 326. 2007
    ..Sensitivity and stability of chemiluminescence immunoassay is very excellent. Fluorescence polarization immunoassay is sensitive and convenient. HPLC-MS-MS has high resolution and good specificity...
  4. ncbi A simple, rapid and high-throughput fluorescence polarization immunoassay for simultaneous detection of organophosphorus pesticides in vegetable and environmental water samples
    Zhen Lin Xu
    Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China
    Anal Chim Acta 708:123-9. 2011
    ..The developed FPIA demonstrated good accuracy and reproducibility, and is suitable for rapid and high-throughput screening for OP contamination with high-efficiency and low cost...
  5. ncbi Assay of teicoplanin in serum: comparison of high-performance liquid chromatography and fluorescence polarization immunoassay
    Steven J McCann
    Department of Clinical Biochemistry, Wythenshawe Hospital, Southmoor Road, Manchester M23 9LT, UK
    J Antimicrob Chemother 50:107-10. 2002
    ..extraction coupled with reverse-phase HPLC and UV detection was shown to correlate well with fluorescence polarization immunoassay (FPIA) on the Abbott TD(x) analyser for serum teicoplanin analysis, r(2) = 0.974, HPLC = 0...
  6. ncbi Application of fluorescence polarization immunoassay for determination of methotrexate-polyglutamates in rheumatoid arthritis patients
    Hideki Hayashi
    Department of Clinical Pharmacology and Genetics, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan
    Tohoku J Exp Med 215:95-101. 2008
    ..We attempted to apply a method with the commercially available fluorescence polarization immunoassay (FPIA) kit...
  7. ncbi The application of CdTe/CdS in the detection of carcinoembryonic antigen by fluorescence polarization immunoassay
    Jianniao Tian
    Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources Ministry of Education of China, College of Chemistry and Chemical Engineering of Guangxi Normal University, Guilin, China
    J Fluoresc 22:1571-9. 2012
    A novel and portable strategy based on fluorescence polarization immunoassay (FPIA) using quantum dots (QDs) was described in this study for simple, rapid, and sensitive detection of carcinoembryonic antigen (CEA)...
  8. ncbi Toxicological detection of the designer drug 3,4-methylenedioxyethylamphetamine (MDE, "Eve") and its metabolites in urine by gas chromatography-mass spectrometry and fluorescence polarization immunoassay
    H K Ensslin
    Pharmaceutical Institute, University of Tubingen, Germany
    J Chromatogr B Biomed Appl 683:189-97. 1996
    ..in urine after a single oral dose of 140 mg of MDE by GC-MS and fluorescence polarization immunoassay (FPIA)...
  9. ncbi Multiplexed detection of tumor markers with multicolor quantum dots based on fluorescence polarization immunoassay
    Jianniao Tian
    Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources Ministry of Education of China, College of Chemistry and Chemical Engineering of Guangxi Normal University, Guilin 541004, China
    Talanta 92:72-7. 2012
    ..nanosensor for multiplex detection of two tumor markers in a homogeneous format based on fluorescence polarization immunoassay was proposed...
  10. ncbi A rapid fluorescence polarization immunoassay for the determination of T-2 and HT-2 toxins in wheat
    Vincenzo Lippolis
    Institute of Sciences of Food Production ISPA, CNR National Research Council of Italy, Via G Amendola 122 o, 70126 Bari, Italy
    Anal Bioanal Chem 401:2561-71. 2011
    ..964). These results, combined with the rapidity (10 min) and simplicity of the assay, show that this method is suitable for high throughput screening as well as for quantitative determination of T-2 and HT-2 toxins in wheat...
  11. ncbi Fluorescence polarization immunoassay: detection of antibody to Brucella abortus
    K Nielsen
    Canadian Food Inspection Agency, Animal Diseases Research Institute, 3851 Fallowfield Road, Nepean, Ontario, K2H 8P9, Canada
    Methods 22:71-6. 2000
    b>Fluorescence polarization immunoassay (FPA) is a homogeneous immunoassay useful for rapid and accurate detection of antibody or antigen...
  12. ncbi Optimization of a fluorescence polarization immunoassay for rapid quantification of deoxynivalenol in durum wheat-based products
    Vincenzo Lippolis
    Institute of Sciences of Food Production ISPA, National Research Council, Via G Amendola 122 o, 70126 Bari, Italy
    J Food Prot 69:2712-9. 2006
    A fluorescence polarization immunoassay previously described for deoxynivalenol (DON) screening in wheat was optimized for the rapid quantification of DON in durum wheat kernels, semolina, and pasta...
  13. ncbi Detection of zearalenone and related metabolites by fluorescence polarization immunoassay
    Chris M Maragos
    Mycotoxin Research Unit, National Center for Agricultural Utilization Research, U S Department of Agriculture, Agricultural Research Service, 1815 North University Street, Peoria, Illinois 61604, USA
    J Food Prot 67:1039-43. 2004
    ..We developed a fluorescence polarization immunoassay for ZEN in maize...
  14. ncbi Fluorescence polarization immunoassay: can it result in an overestimation of vancomycin in patients not suffering from renal failure?
    D Sym
    St John s University, College of Pharmacy and Allied Health Professions, Jamaica, New York, USA
    Ther Drug Monit 23:441-4. 2001
    It has been reported in scientific data that fluorescence polarization immunoassay (FPIA) results in overestimation of vancomycin in patients with renal failure...
  15. ncbi Determination of everolimus in whole blood using the Abbott IMx sirolimus microparticle enzyme immunoassay
    Lorena Bouzas
    Unidad Monitorización Fármacos, Laboratorio Central, Hospital Clinico Universitario, Santiago de Compostela, Spain
    Clin Biochem 40:132-6. 2007
    ..The aim of our study was to evaluate the possible determination of everolimus in whole blood using a commercialized microparticle enzyme immunoassay for sirolimus determination...
  16. ncbi False-positive serum tricyclic antidepressant concentrations using fluorescence polarization immunoassay due to the presence of hydroxyzine and cetirizine
    Amitava Dasgupta
    Department of Pathology and Laboratory Medicine, University of Texas Medical School at Houston, Houston, Texas 77030, USA
    Ther Drug Monit 29:134-9. 2007
    ..The potential interference of hydroxyzine and cetirizine with the fluorescence polarization immunoassay (FPIA) and CEDIA assay of carbamazepine as well as with the fluorescence polarization immunoassay ..
  17. ncbi Rapid fluorescence polarization immunoassay for the mycotoxin deoxynivalenol in wheat
    Chris M Maragos
    Mycotoxin Research Unit, National Center for Agricultural Utilization Research, USDA ARS, 1815 North University Street, Peoria, Illinois 61604, USA
    J Agric Food Chem 50:1827-32. 2002
    ..For wheat the HPLC-UV and FP methods agreed well (linear regression r(2) = 0.936), but for maize the two methods did not (r (2) = 0.849). We conclude that the FP method is useful for screening wheat, but not maize, for DON...
  18. ncbi Comparison of the Innofluor certican assay with HPLC-UV for the determination of everolimus concentrations in heart transplantation
    Sara Baldelli
    Department of Medicine and Transplantation, Ospedali Riuniti di Bergamo Mario Negri Institute for Pharmacological Research, Bergamo, Italy
    Clin Biochem 39:1152-9. 2006
    ..A fluorescence polarization immunoassay (FPIA) has been recently developed for the assessment of everolimus levels...
  19. pmc Determination of blood sirolimus concentrations in liver and kidney transplant recipients using the Innofluor fluorescence polarization immunoassay: comparison with the microparticle enzyme immunoassay and high-performance liquid chromatography-ultraviole
    Lorena Bouzas
    Unidad Monitorización Fármacos, Laboratorio Central, Hospital Clinico Universitario, Santiago de Compostela, Spain
    Ups J Med Sci 114:55-61. 2009
    ..The aim of our study was to evaluate the possible determination of sirolimus using the fluorescence polarization immunoassay (FPIA) commercialized for everolimus quantification.
  20. ncbi Fluorescence polarization immunoassay based on a monoclonal antibody for the detection of the organophosphorus pesticide parathion-methyl
    Anna Yu Kolosova
    Division of Applied Life Science, Graduate School of Gyeongsang National University, Chinju, Gyeongnam 660 701, Korea
    J Agric Food Chem 51:1107-14. 2003
    A fluorescence polarization immunoassay (FPIA) based on a monoclonal antibody for the detection of parathion-methyl (PM) was developed and optimized...
  21. ncbi Amphetamine concentrations in human urine following single-dose administration of the calcium antagonist prenylamine-studies using fluorescence polarization immunoassay (FPIA) and GC-MS
    Thomas Kraemer
    Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, University of Saarland, D 66421 Homburg Saar, Germany
    J Anal Toxicol 27:68-73. 2003
    ..Chiral analysis revealed that both enantiomers of amphetamine were present in the samples with a surplus of the S(+)-enantiomer in the early phase of excretion. Forensic implications are discussed...
  22. ncbi Positive and negative in vitro interference of Chinese medicine dan shen in serum digoxin measurement. Elimination of interference by monitoring free digoxin concentration
    A Wahed
    Dept of Pathology and Laboratory Medicine, University of Texas Houston Medical School, 6431 Fannin, MSB 2.292, Houston, TX 77030, USA
    Am J Clin Pathol 116:403-8. 2001
    ..in falsely elevated serum digoxin concentrations (positive interference) as measured by the fluorescence polarization immunoassay for digoxin (Abbott Laboratories, Abbott Park, IL)...
  23. ncbi Diagnostic efficiency of different amphetamine screening tests--the search for an optimal cutoff
    Reto Savoca
    Center of Laboratory Medicine, Kantonsspital Aarau, Aarau, Switzerland
    Clin Chem Lab Med 42:1063-5. 2004
    ..In summary, the cutoff concentration for amphetamine screening tests should not be below 500 ng/ml to avoid a high rate of false-positive results...
  24. ncbi Between-centre agreement in homocysteine assays: experience from the UK NEQAS proficiency testing scheme
    I Jennings
    Thromb Haemost 87:921-2. 2002
  25. ncbi Comparison of fluorescence polarization immunoassay and high performance liquid chromatography for determination of carbamazepine concentration in blood of poisoned patients
    Jolanta Wilimowska
    Department of Analytical Toxicology, Jagiellonian University Medical College, Krakow, Poland
    Przegl Lek 62:595-8. 2005
    ..There was no statistical difference between serum carbamazepine concentration measured by FPIA method and the sum of serum carbamazepine and carbamazepine-10,11-epoxide concentrations measured by HPLC-DAD method (t21 = 1.42, p = 0.1690)...
  26. ncbi Towards single screening tests for brucellosis
    K Nielsen
    Animal Diseases Research Institute, Canadian Food Inspection Agency, 3851 Fallowfield Rd, Nepean, Ontario, K2H 8P9, Canada
    Rev Sci Tech 24:1027-37. 2005
    ..2%) than from only the OPS, and considerably higher (12.6%) than the CORE. Overall, both the I-ELISA and the FPA with combined antigens were suitable as screening tests for all species of Brucella in the animal species tested...
  27. ncbi Flow injection fluorescence immunoassay for gentamicin using sol-gel-derived mesoporous biomaterial
    Huang Hao Yang
    Department of Chemistry, The Key Laboratory of Analytical Sciences of MOE, Xiamen University, People s Republic of China
    Anal Biochem 308:71-6. 2002
    ..with a detection limit of 200 ng/mL, which is close to that of the fluorescence immunoassay and fluorescence polarization immunoassay using the same reactants...
  28. ncbi Comparing different methods for homocysteine determination
    Bruno Zappacosta
    Istituto di Biochimica e Biochimica Clinica, Universita Cattolica del Sacro Cuore, Rome, Italy
    Clin Chem Lab Med 40:1139-42. 2002
    ..The results clearly indicate that all three automated immunochemical methods correlate well with the HPLC method (slope 0.97-1.03; intercept 0.95-1.91 with a recovery above 95% for all three methods)...
  29. ncbi Digitalis-like immunoreactive substances in maternal and umbilical cord plasma: a comparative sensitivity study of fluorescence polarization immunoassay and microparticle enzyme immunoassay
    Yoshio Ijiri
    Department of Pharmacy, Osaka Medical College Hospital, Takatsuki, Osaka, Japan
    Ther Drug Monit 25:234-9. 2003
    ..substances (DLIS) obtained from maternal and umbilical cord plasma at delivery were measured by fluorescence polarization immunoassay (FPIA; TDX, Abbott) and microparticle enzyme immunoassay (MEIA; IMX, Abbott)...
  30. ncbi Comparison of fluorescence polarization immunoassay and high-performance liquid chromatography methods for assay of teicoplanin: can correlation be improved?
    Siamak Davani
    Laboratoire de Pharmacologie Toxicologie, CHU Jean Minjoz, 3, Boulevard Fleming, 25000 Besancon, France
    Pathol Biol (Paris) 52:584-8. 2004
    ..Measurement of teicoplanin can be carried out by the fluorescence polarization immunoassay (FPIA) method or by high-performance liquid chromatography (HPLC) methods...
  31. ncbi Stability of oleander extract and oleandrin in sera stored in plastic serum separator tubes
    Amitava Dasgupta
    Department of Pathology and Laboratory Medicine, University of Texas Health Sciences Center in Houston, Houston, Texas, USA
    Ann Clin Biochem 44:485-7. 2007
    ..The fluorescence polarization immunoassay (FPIA) for digoxin cross-reacts with oleandrin, the major poison of oleander extract and can be ..
  32. ncbi Receptor assay based on surface plasmon resonance for the assessment of the complex formation activity of cyclosporin A and its metabolites
    B Vollenbroeker
    Medizinische Klinik und Poliklinik D, Munster, Germany
    Int J Clin Pharmacol Ther 41:248-60. 2003
    ..A new automated receptor assay has been used to determine the complex formation activity of cyclosporin A (CsA) and its metabolites in whole blood...
  33. ncbi Analytical performance of the CEDIA cyclosporine PLUS whole blood immunoassay
    Anthony W Butch
    Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California 90095 1713, USA
    J Anal Toxicol 28:204-10. 2004
    ..high-performance liquid chromatography (HPLC), enzyme-multiplied immunoassay technique (EMIT), and fluorescence polarization immunoassay (FPIA)...
  34. ncbi Serological diagnosis of bovine brucellosis: a review of test performance and cost comparison
    D Gall
    Canadian Food Inspection Agency, Animal Diseases Research Institute, Brucellosis Centre of Expertise, Ottawa, Ontario, K2H 8P9, Canada
    Rev Sci Tech 23:989-1002. 2004
    ..In addition, a fee comparison suggested that the primary binding tests were price competitive with conventional tests for the diagnosis of brucellosis and, therefore, had a better combined cost/efficiency rating...
  35. ncbi Validation of fluorescence polarization assay (FPA) and comparison with other tests used for diagnosis of B. melitensis infection in sheep
    A Minas
    Technological Educational Institution of Larissa, Faculty of Health Professions, Laboratory of Microbiology, Larissa, 41 110, Greece
    Vet Microbiol 111:211-21. 2005
    ..Due to its simplicity, the sort time that results can be obtained and its accuracy it can be used and improve the laboratory testing capacity as well as the efficacy of the eradication program based on test-and-slaughter policy...
  36. ncbi Improved performance of Brucella melitensis native hapten over Brucella abortus OPS tracer on goat antibody detection by the fluorescence polarization assay
    C Ramirez-Pfeiffer
    Instituto Nacional de Investigaciones Forestales y Agropecuarias, Campo Experimental Río Bravo, Rio Bravo, Tamaulipas, Mexico
    Vet Immunol Immunopathol 123:223-9. 2008
    ..24-72h) than the immunodiagnosis assays frequently used in bovine brucellosis. In addition, NH tracer produces similar or better performance results than the conventional OPS tracer, using the FPA in goat sera samples...
  37. ncbi Comparison between a liquid chromatography-tandem mass spectrometry assay and a fluorescent polarization immunoassay to measure whole blood everolimus concentration in heart and renal transplantations
    Eric Dailly
    Clinical Pharmacology Department, Hotel Dieu, CHU de Nantes, France
    J Clin Lab Anal 22:282-5. 2008
    ..FPIA and LC-MS/MS assays gave consistent overall results although some significant differences were observed in some samples between these methods indicating that FPIA assay has limitations that deserve further investigations...
  38. ncbi [Validation of the Fluorescence Polarisation Assay (FPA) for the serological diagnosis of brucellosis]
    Peter Bahn
    Bundesinstitut für Risikobewertung, Berlin
    Berl Munch Tierarztl Wochenschr 118:372-6. 2005
    ..FPA, SAT, CFT and ELISA attained a sensitivity of 92.3, 98.5, 84.6 and 86.2%. In comparison, specificity varied with 87.8, 72.6, 92.5 and 85.8%, respectively. Accordingly, FPA is a suitable test for serodiagnosis of brucellosis...
  39. ncbi Major discrepancy between digoxin immunoassay results in a context of acute overdose: a case report
    Olivier Tribut
    Clin Chim Acta 354:201-3. 2005
  40. ncbi Comparison of serum digoxin concentration monitoring by fluorescence polarization immunoassay on the TDxFLx and dry chemistry enzyme immunoassay on the Vitros 950
    Bogdan Solnica
    Diagnostic Division, Department of Clinical Biochemistry, Jagiellonian University, Collegium Medicum, Krakow, Poland
    Clin Chem Lab Med 42:958-64. 2004
    The aim of the study was to compare the results of digoxin assays performed using fluorescence polarization immunoassay (FPIA) on the TDxFLx and a dry chemistry enzyme immunoassay (EIA) on the Vitros 950. Within-run CV amounted to 8...
  41. ncbi [Detection of cannabinoids in urine]
    S S Kataev
    Sud Med Ekspert 48:35-8. 2005
    ..A possibility was pointed out to apply the methods of ICA and TLC as system analysis in the determination of 11-nordelta-9-tetrahydrocannabinol-9-carbonic acid in urine of marijuana users...
  42. ncbi Homocysteine and retinal emboli: the Blue Mountains Eye Study
    Brian Chua
    Centre for Vision Research, Department of Ophthalmology, University of Sydney, Sydney, Australia
    Am J Ophthalmol 142:322-4. 2006
    ..To examine the association between serum homocysteine and retinal emboli in a general older population...
  43. ncbi Clinical validation of a new blood collection tube for the accuracy of total homocysteine measurement by different methods
    Emmanuel Bisse
    Department of Clinical Chemistry, University Medical Center Freiburg, Hugstetterstrasse 55, D 79106 Freiburg, Germany
    Clin Biochem 40:739-43. 2007
    ..To evaluate the clinical use of Homocysteine-Primavette, a new blood collection medium for total homocysteine (tHcy) assay...
  44. pmc Use of the Brucella melitensis native hapten to diagnose brucellosis in goats by a rapid, simple, and specific fluorescence polarization assay
    Carlos Ramírez-Pfeiffer
    Campo Experimental Río Bravo, Instituto Nacional de Investigaciones Forestales y Agrícolas y Pecuarias, Rio Bravo, Tam, Mexico
    Clin Vaccine Immunol 15:911-5. 2008
    ....
  45. ncbi Fluorescence polarization assay for diagnosis of human brucellosis
    Nidia E Lucero
    Brucellosis Laboratory, Administracion Nacional de Laboratorios e Institutos de Salud Dr C G Malbran, Avda Velez Sarsfield 563, 1281 Buenos Aires, Argentina
    J Med Microbiol 52:883-7. 2003
    b>Fluorescence polarization immunoassay (FPA) uses molecular rotational properties to measure antibody binding to antigen directly...
  46. pmc Application of the fluorescence polarization assay for detection of caprine antibodies to Brucella melitensis in areas of high prevalence and widespread vaccination
    C Ramirez-Pfeiffer
    Río Guadalquivir 401 B Ote, Colonia del Valle, San Pedro Garza Garcia, Nuevo Leon, Mexico, C P 66220
    Clin Vaccine Immunol 14:299-303. 2007
    ....
  47. ncbi Evaluation and comparison of fluorescence polarization assay with three of the currently used serological tests in diagnosis of human brucellosis
    A Konstantinidis
    Department of Hygiene and Epidemiology, Medical School, University of Thessaly, 22 Papakiriazi Str, PC 41222, Larissa
    Eur J Clin Microbiol Infect Dis 26:715-21. 2007
    ..However, further studies are needed to assess the reproducibility of FPA...
  48. ncbi [Antidepressants]
    Kimio Yasuda
    Department of Pharmacy, Hashima City Hospital
    Nihon Rinsho 62:372-6. 2004
  49. ncbi Hoek's formula, a cystatin C-based prediction formula for determining the glomerular filtration rate, is the most effective method for original adjusting the dosage of vancomycin
    A Tanaka
    Division of Pharmacy, Ehime University Hospital, Ehime, Japan
    Int J Clin Pharmacol Ther 45:592-7. 2007
    ..In this study, we compared the predictive performance of the serum vancomycin trough concentration predicted using serum cystatin C-based formulas...
  50. ncbi Effect of analytical inaccuracy on dose adjustment for gentamicin using the Abbottbase Pharmacokinetic Systems
    Lorena Bouzas
    Laboratorio Central, Hospital Clinico Universitario, Santiago de Compostela, Spain
    Clin Lab 50:187-91. 2004
    ..For this reason, we would suggest the convenience of using a previous moment in the time concentration level in the monitoring of gentamicin instead of Cmin, which would be determined with greater imprecision and inaccuracy...
  51. ncbi Interference by carbamazepine and oxcarbazepine with serum- and urine-screening assays for tricyclic antidepressants
    Mohsen Saidinejad
    Division of Emergency Medicine, Children s Hospital Boston, Boston, MA 02115, USA
    Pediatrics 120:e504-9. 2007
    ..The purpose of this work was to evaluate the potential cross-reactivity of 2 antiepileptic medications containing 3-ringed structures, namely, carbamazepine and oxcarbazepine, with screening assays for tricyclic antidepressants...
  52. ncbi Total plasma homocysteine in Bulgarian population measured by high-performance liquid chromatography with fluorescence detection--comparison with fluorescence polarization immunoassay
    Evgeniya A Angelova
    Department of Clinical Laboratory, University of Medicine, Plovdiv, Bulgaria
    Clin Lab 52:527-33. 2006
    ..The higher plasma Hcy observed in our population compared to the rest of Europe corresponds to the high prevalence and mortality of cardiovascular disease in Bulgaria...
  53. ncbi Microfluidic enzyme immunosensors with immobilised protein A and G using chemiluminescence detection
    Julia Yakovleva
    Department of Chemical Enzymology, Faculty of Chemistry, M V Lomonosov Moscow State University, Moscow 119899, Russia
    Biosens Bioelectron 19:21-34. 2003
    ..5-3 and 80-240 microg l(-1), respectively...
  54. ncbi Analysis of apoptotic cells using Beadlyte suspension arrays
    Paul W Rhyne
    Upstate USA, Lake Placid, NY, USA
    Biotechniques 35:624-9. 2003
    ..The suspension array is being expanded to measure many other intracellular proteins including posttranslational modifications and should prove to be extremely useful for studying apoptosis and other important cellular pathways...
  55. ncbi Integrated capillary fluorescence DNA biosensor
    Marc A Breimer
    Department of Chemistry, State University of New York at Binghamton, PO Box 6016, Binghamton, NY 13902 6016, USA
    Biosens Bioelectron 18:1135-47. 2003
    ..The results show that fluorescent dyes are retained in the dsDNA-modified surface and that exposure to concentrations of nickel and lead ions resulted in a recoverable, highly reproducible diminishment of the fluorescence intensity...
  56. ncbi Fluorescence polarization immunoassays and related methods for simple, high-throughput screening of small molecules
    David S Smith
    MicroPharm Ltd, 51 53 Bartholomew Close, St Bartholomew s Hospital, London, EC1A 7BE, UK
    Anal Bioanal Chem 391:1499-507. 2008
    b>Fluorescence polarization immunoassay (FPIA) is a homogeneous (without separation) competitive immunoassay method based on the increase in fluorescence polarization (FP) of fluorescent-labeled small antigens when bound by specific ..
  57. ncbi Evaluation of a compact bench top immunoassay analyzer for automatic and near continuous monitoring of a sample for environmental contaminants
    Thomas R Glass
    Department of Bioscience, Central Research Institute of the Electric Power Industry, 1646 Abiko, Abiko City, Chiba 270 1194, Japan
    Biosens Bioelectron 20:397-403. 2004
    ..With further development and characterization, the bench top analyzer is expected to fill an important niche in environmental testing...
  58. ncbi Detection of trophic factor activated signaling molecules in cells by a compact fiber-optic sensor
    Rakesh Kapoor
    Institute for Lasers, Photonics and Biophotonics, University at Buffalo, State University of New York, 458 NSC, Buffalo, NY 14260, USA
    Biosens Bioelectron 20:345-9. 2004
    ..A compact charged coupled device (CCD) based spectrometer is used for recording the fluorescence signal. The method is two orders of magnitude more sensitive than the Western blotting technique...
  59. ncbi B-type natriuretic peptide rapid assay: a diagnostic test for heart failure
    Irma B Ancheta
    University of Phoenix, Jacksonville, FL 32218, USA
    Dimens Crit Care Nurs 25:149-54; quiz 155-6. 2006
    ..This article focuses on the description of the diagnostic performance of the BNP rapid assay, its clinical dimensions, and its implications to nursing practice and collaborative practice models...
  60. ncbi Express detection of pentachlorophenol as dioxins precursor in natural water
    Vitalia S Krikounova
    Faculty of Chemistry, M V Lomonosov Moscow State University, Moscow
    ScientificWorldJournal 2:1132-7. 2002
    ..The assay provides a useful and a highly practical screening tool for the processing of large numbers of samples and for the preliminary estimation of potential dioxins contamination in water resources...
  61. ncbi In situ measurement of degranulation as a biosensor based on RBL-2H3 mast cells
    Rose Mary Z G Naal
    Baker Laboratory, Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853 1301, USA
    Biosens Bioelectron 20:791-6. 2004
    ..We also demonstrate its utility in detecting inhibition of degranulation by a known pharmacologic inhibitor that blocks Syk tyrosine kinase activity critical for cell activation...
  62. ncbi Highly sensitive biosensing using a supercritical angle fluorescence (SAF) instrument
    Thomas Ruckstuhl
    Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstr 190, CH 8057, Zurich, Switzerland
    Biosens Bioelectron 18:1193-9. 2003
    ..The performance of the system is demonstrated by real time measurements of antibody-antigen reactions. Rate constants of the reaction were extracted. Antigen concentrations were detected down to 10(-13) mol/l...
  63. ncbi Verification of performance with the automated direct optical TIRF immunosensor (River Analyser) in single and multi-analyte assays with real water samples
    Jens Tschmelak
    Institute of Physical and Theoretical Chemistry IPTC, Eberhard Karls University of Tuebingen, Auf der Morgenstelle 8, D 72076 Tuebingen, Germany
    Biosens Bioelectron 20:743-52. 2004
    ..Robustness could be demonstrated by long-term stability tests of the biosensor surface. These studies show that the biosensor used offers the necessary reproducibility, precision, and robustness required for an analytical method...
  64. ncbi High-sensitivity miniaturized immunoassays for tumor necrosis factor alpha using microfluidic systems
    Sandro Cesaro-Tadic
    IBM Research GmbH, Zurich Research Laboratory, Saumerstrasse 4, CH 8803 Ruschlikon, Switzerland
    Lab Chip 4:563-9. 2004
    ..6 mm(2) on PDMS to detect TNF-alpha in 600 nL of a dendritic cell (DC) culture medium with a sensitivity of approximately 20 pg mL(-1)(1.14 pM)...
  65. ncbi A rapid detection method for Vaccinia virus, the surrogate for smallpox virus
    Kim A Donaldson
    Department of Biology and Center for Biological Defense, University of South Florida, 4202 East Fowler Avenue, SCA 110, Tampa, FL 33620 5200, USA
    Biosens Bioelectron 20:322-7. 2004
    ..A photodiode quantifies the emission light at wavelengths between 670 and 710 nm. The biosensor was able to detect a minimum of 2.5 x 10(5) pfu/ml of Vaccinia virus in seeded throat culture swab specimens...
  66. ncbi One-step quantitative thyrotropin assay for the detection of hypothyroidism in point-of-care conditions
    Piia von Lode
    Department of Biotechnology, University of Turku, Tykistokatu 6A, FIN 20520, Turku, Finland
    Clin Biochem 36:121-8. 2003
    ..We demonstrate the applicability of a miniaturized microparticle assay format for rapid and quantitative determination of increased thyrotropin (TSH) concentrations in serum...
  67. ncbi Development of fluorescence change-based, reagent-less optic immunosensor
    Satoka Aoyagi
    Department of Applied Physics, Seikei University, 3 3 1, Kichijoji Kitamachi, Musashino shi, Tokyo 180 8633, Japan
    Biosens Bioelectron 20:1680-4. 2005
    ..When the Qdot-labeled protein A bound to IgG, fluorescence intensity decreased because of the inhibition by IgG. The degree of fluorescence decrease depends on the IgG concentration at a steady state and also in a transient state...
  68. ncbi Development of a novel FRET immunosensor technique
    Darcy J Lichlyter
    Department of Biological Engineering, University of Missouri Columbia, 250 Ag Engineering Building, Columbia, MO 65211, USA
    Biosens Bioelectron 19:219-26. 2003
    ..The feasibility of the FRET immunosensor technique was established; however, it will be necessary to immobilize the complexes onto optical substrates so that consistent trends can be obtained that would allow calibration plots...
  69. ncbi Stable, sensitive, fluorescence-based method for detecting cAMP
    Jayne Hesley
    Molecular Devices, Sunnyvale, CA 94089, USA
    Biotechniques 33:691-4. 2002
    ..Together, these results demonstrate that this assay is afast, accurate, non-radioactive method that is ideal for high-throughput screening...
  70. ncbi Analysis of codeine, dihydrocodeine and their glucuronides in human urine by electrokinetic capillary immunoassays and capillary electrophoresis-ion trap mass spectrometry
    A B Wey
    Department of Clinical Pharmacology, University of Berne, Switzerland
    J Chromatogr A 895:133-46. 2000
    ..In these approaches, aliquots of urine and immunoreagents of a commercial, broadly cross-reacting fluorescence polarization immunoassay for opiates were combined and analyzed by capillary zone electrophoresis or micellar ..
  71. ncbi Pharmacokinetics of nimustine, cytosine arabinoside, and methotrexate in cerebrospinal fluid during cerebrospinal fluid perfusion chemotherapy
    N Morikawa
    Department of Clinical Pharmacy, Oita Medical University, Japan
    Biol Pharm Bull 24:436-8. 2001
    ..The concentrations of ACNU and Ara-C in CSF were measured by HPLC, and the MTX concentrations by fluorescence polarization immunoassay. In the fifth course of treatment, a CSF injection chemotherapy solution, consisting of 5 mg of ..
  72. ncbi Interference between eplerenone and digoxin in fluorescence polarization immunoassay, microparticle enzyme immunoassay, and affinity column-mediated immunoassay
    Tomoyuki Yamada
    Department of Pharmacy, Osaka Medical College Hospital, Osaka, Japan
    Ther Drug Monit 32:774-7. 2010
    ..We performed three types of assays (fluorescence polarization immunoassay [FPIA], microparticle enzyme immunoassay [MEIA], and affinity column-mediated immunoassay [ACMIA]) ..
  73. ncbi [Cyclosporine 2% eye drops preparation]
    F Chast
    Service de Pharmacie, Pharmacologie et Toxicologie, Hotel Dieu, Paris
    J Fr Ophtalmol 27:567-76. 2004
    ..Moreover, it is widely used in the case of high-risk rejection corneal transplantation. We present a preparation of cyclosporine 2% eye drops...
  74. ncbi An improved application for the enzyme multipled immunoassay technique for caffeine, amikacin, and methotrexate assays on the Dade-Behring Dimension RxL Max clinical chemistry system
    Damodara Rao Mendu
    Department of Medicine, Georgetown University and Georgetown Clinical Research Center, Washington, DC, USA
    Ther Drug Monit 29:632-7. 2007
    ..and MTX by EMIT on the Dimension RxL Max and by EMIT (on the Olympus AU 600 for caffeine) and fluorescence polarization immunoassay [for MTX and amikacin (FPIA; TDx FLx)] at Quest Diagnostics...
  75. ncbi Effects of genetic polymorphisms of CYP3A4, CYP3A5 and MDR1 on cyclosporine pharmacokinetics after renal transplantation
    Yong fang Hu
    Peking University Third Hospital, Beijing, China
    Clin Exp Pharmacol Physiol 33:1093-8. 2006
    ..Cyclosporine whole blood levels were measured by fluorescence polarization immunoassay. Dose-adjusted trough blood concentrations (C(0)) were determined and compared among the different ..
  76. ncbi Pharmacokinetics of cytosine arabinoside, methotrexate, nimustine and valproic acid in cerebrospinal fluid during cerebrospinal fluid perfusion chemotherapy
    N Morikawa
    Department of Clinical Pharmacy, Oita Medical University, Japan
    Biol Pharm Bull 23:784-7. 2000
    ..The MTX and VPA concentrations were measured by fluorescence polarization immunoassay. During CSF perfusion chemotherapy, the highest CSF concentrations of Ara-C, MTX, and ACNU were ..
  77. ncbi Clinical repercussions of analytical interferences due to aldosterone antagonists in digoxin immunoassays: an assessment
    Ana Cobo
    Department of Pharmacy and Pharmaceutical Technology, University of Salamanca, Salamanca, Spain
    Ther Drug Monit 32:169-76. 2010
    ..in comparison with another 3 immunoassays used for digoxin therapeutic drug monitoring: MEIA II, fluorescence polarization immunoassay, and enzyme multiplied immunoassay...
  78. ncbi Screening for urinary methadone by capillary electrophoretic immunoassays and confirmation by capillary electrophoresis-mass spectrometry
    W Thormann
    Department of Clinical Pharmacology, University of Bern, Switzerland
    Electrophoresis 19:57-65. 1998
    ..capillary-based immunoassays for urinary methadone using reagents which were commercialized for a fluorescence polarization immunoassay. After incubation of 25 microL urine with the reactants, a small aliquot of the mixture is applied ..
  79. ncbi Plasma homocysteine in polycystic ovary syndrome: does it correlate with insulin resistance and ethnicity?
    Chandrika N Wijeyaratne
    Department of Obstetrics and Gynaecology, Faculty of Medicine, University of Columbo, Sri Lanka
    Clin Endocrinol (Oxf) 60:560-7. 2004
    ..Hyperhomocysteinaemia is a recognized risk factor for atherosclerosis, particularly among migrant South Asians, and has recently been shown to be correlated positively with the degree of insulin resistance/hyperinsulinaemia...
  80. ncbi Dose-related increases in cerebrospinal fluid concentrations of methotrexate in a postoperative patient with glioblastoma
    N Morikawa
    Department of Clinical Pharmacy, Oita Medical University, Hasama machi, Japan
    Ann Pharmacother 33:952-6. 1999
    ....
  81. ncbi [Optimalisation of netilmicin dosage based on therapeutic drug monitoring during the first days of life in very preterm neonates (gestational age 23-32 weeks)]
    Magdalena Rutkowska
    Klinika Neonatologii i Intensywnej Terapii Noworodka, IMiD, Instytutu Matki i Dziecka w Warszawie, 01 211 Warszawa
    Med Wieku Rozwoj 13:252-9. 2009
    ..The aim of this study was to optimalise netilmicin dosage in low and very low birthweight premature neonates, based on drug serum concentrations obtained during therapeutic drug monitoring (TDM)...
  82. ncbi Pharmacokinectics of vancomycin and amikacin in the subeschar tissue fluid in patients with severe burn
    Rong Hua Yang
    Department of Burns, The First Municipal Hospital of Guangzhou, Guangzhou Medical College, Guangzhou, China
    Burns 35:75-9. 2009
    ..Concentrations of these antibiotics in the samples were determined by fluorescence polarization immunoassay (FPIA) method...
  83. ncbi Analgesic efficacy of sodium salicylate in an amphotericin B-induced bovine synovitis-arthritis model
    J L Kotschwar
    Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas 66506 5601, USA
    J Dairy Sci 92:3731-43. 2009
    ..Cortisol and salicylate samples were analyzed by competitive chemiluminescent immunoassay and fluorescence polarization immunoassay, respectively. The pharmacokinetic data were analyzed using compartmental analysis...
  84. ncbi Analysis of risk factors for tumor recurrence after liver transplantation for hepatocellular carcinoma: key role of immunosuppression
    Marco Vivarelli
    Department of Surgery and Transplantation, University of Bologna, S Orsola Hospital, Bologna, Italy
    Liver Transpl 11:497-503. 2005
    ..CsA trough blood levels, measured with the same technique (fluorescence polarization immunoassay), were analyzed at different time points after transplantation...
  85. ncbi Inhibition of N-acetylation of procainamide and renal clearance of N-acetylprocainamide by para-aminobenzoic acid in humans
    J E Tisdale
    College of Pharmacy and Allied Health Professions, Wayne State University, Detroit, Michigan 48202, USA
    J Clin Pharmacol 35:902-10. 1995
    ..Serum procainamide and NAPA concentrations were analyzed using fluorescence polarization immunoassay. Urine procainamide and NAPA concentrations were measured with high performance liquid ..
  86. ncbi [Plasma homocysteine level and selected dietary habits in young healthy men]
    Anna Czajkowska
    Zakład Fizjologii, Akademia Wychowania Fizycznego w Warszawie
    Rocz Panstw Zakl Hig 60:85-9. 2009
    ..Plasma homocysteine concentration was determined by fluorescence polarization immunoassay using commercial kits from Abbott (USA)...
  87. ncbi Intraosseous gentamicin perfusion of the distal metacarpus in standing horses
    Shawn Mattson
    Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Canada
    Vet Surg 33:180-6. 2004
    ..To report tissue gentamicin concentrations after intraosseous (IO) perfusion in standing horses...
  88. ncbi Homogenous enzyme immunoassay for cyclosporine in whole blood using the EMIT 2000 cyclosporine specific assay with the COBAS MIRA-plus analyzer
    S Kimura
    Laboratory for Clinical Investigation, Osaka University Hospital, Suita, Osaka, Japan
    J Clin Lab Anal 15:319-23. 2001
    ..The correlation between values obtained with the EMIT 2000 cyclosporine specific assay kit (y) and fluorescence polarization immunoassay (FPIA) (TDxFLx) (x) was: y = 0.880x - 13.053 microg/L (r = 0.984, Sy/x = 15...
  89. ncbi Increased cannabinoids concentrations found in specimens from fatal aviation accidents between 1997 and 2006
    Dennis V Canfield
    Civil Aerospace Medical Institute, Federal Aviation Administration, Oklahoma City, OK 73125 5066, USA
    Forensic Sci Int 197:85-8. 2010
    ..Cannabinoids were screened using radioimmunoassay (RIA) for blood and fluorescence polarization immunoassay (FPIA) for urine and confirmed using GC/MS...
  90. ncbi Effect of Chinese medicines Chan Su and Lu-Shen-Wan on serum digoxin measurement by Digoxin III, a new digoxin immunoassay
    Meredith A Reyes
    Department of Pathology and Laboratory Medicine, University of Texas Health Sciences Center at Houston, Houston, Texas 77030, USA
    Ther Drug Monit 30:95-9. 2008
    ..and Lu-Shen-Wan with the Digoxin III assay by comparing results obtained by using Digoxin II and fluorescence polarization immunoassay, also manufactured by Abbott Laboratories...
  91. ncbi Lack of interaction of digoxin and P-glycoprotein inhibitors, quinidine and verapamil in human placenta in vitro
    Gershon Holcberg
    Department of Obstetrics and Gynecology, Soroka University Medical Center, Faculty of Health Sciences, Ben Gurion University of the Negev, PO Box 151, Beer Sheva 84101, Israel
    Eur J Obstet Gynecol Reprod Biol 109:133-7. 2003
    ..To determine the effect of quinidine and verapamil, known antiarrhythmic agents and P-glycoprotein (Pgp) inhibitors, on digoxin transport from the maternal to the fetal compartment in the isolated perfused human placenta...
  92. ncbi Correlations between calcineurin phosphatase inhibition and cyclosporine metabolites concentrations in kidney transplant recipients: implications for immunoassays
    Nikolaos Karamperis
    Research Laboratory C, Department of Renal Medicine C, Skejby, Aarhus University Hospital, Aarhus, Denmark
    Basic Clin Pharmacol Toxicol 98:569-74. 2006
    ..was determined by the enzyme multiplied immunoassay technique (EMIT) and by the polyclonal fluorescence polarization immunoassay (pFPIA)...
  93. ncbi Interference of Asian, American, and Indian (Ashwagandha) ginsengs in serum digoxin measurements by a fluorescence polarization immunoassay can be minimized by using a new enzyme-linked chemiluminescent immunosorbent or turbidimetric assay
    Amitava Dasgupta
    Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, 6431 Fannin, MSB 2 292, Houston, TX 77030, USA
    Arch Pathol Lab Med 131:619-21. 2007
    Ginsengs are widely used by the general population. These herbs interfere with serum digoxin measurement using the fluorescence polarization immunoassay.
  94. ncbi Insulin resistance in patients with polycystic ovary syndrome is associated with elevated plasma homocysteine
    Morey Schachter
    IVF and Infertility Unit, Department of Obstetrics and Gynecology, Assaf Harofeh Medical Center, Tel Aviv University, Zerifin 70300, Israel
    Hum Reprod 18:721-7. 2003
    ..We examined the relationship between plasma homocysteine and insulin resistance in patients with polycystic ovary syndrome (PCOS)...
  95. ncbi Phenytoin immunoassay measurements in serum samples from patients with renal insufficiency: comparison with high-performance liquid chromatography
    Maria J Tutor-Crespo
    Laboratorio Central, Hospital Clinico Universitario, Santiago de Compostela, Spain
    J Clin Lab Anal 21:119-23. 2007
    ..The aim of this study was to compare the results obtained using the Abbott fluorescence polarization immunoassay (FPIA), Dade enzyme-multiplied immunoassay technique (EMIT), and high-performance liquid ..
  96. ncbi Reassessment of cross-reactivity of spironolactone metabolites with four digoxin immunoassays
    R A Pleasants
    Department of Pharmacy, North Carolina Memorial Hospital, Chapel Hill
    Ther Drug Monit 11:200-4. 1989
    ..Samples were analyzed in duplicate by each of the following immunoassays: fluorescence polarization immunoassay (FPIA); affinity-column-mediated immunoassay (ACMIA); radioimmunoassay (RIA); and enzyme ..
  97. ncbi Validated gas chromatographic-mass spectrometric analysis of urinary cannabinoids purified with a calcium-hardened beta-cyclodextrin polymer
    Ju Yeon Moon
    Life Sciences Division, Korea Institute of Science and Technology, 39 1 Hawolkok dong, Seoul 136 791, South Korea
    J Chromatogr A 1204:87-92. 2008
    ..9997). Twenty actual samples positive by fluorescence polarization immunoassay were also quantitatively analyzed...
  98. ncbi Comparison of phenytoin serum concentrations in premature neonates following intravenous and oral administration
    O R Frey
    Department of Pharmacy, Hospital of Heidenheim, Germany
    Ann Pharmacother 32:300-3. 1998
    ..To compare the serum concentrations attained following intravenous and oral administration of phenytoin in premature neonates...
  99. ncbi Prenatal diagnosis of methylmalonic aciduria by analysis of organic acids and total homocysteine in amniotic fluid
    Yao Zhang
    Department of Pediatrics, Peking University First Hospital, Beijing 100034, China
    Chin Med J (Engl) 121:216-9. 2008
    ..The aim of this study was to determine the value of analysis of organic acids and total homocysteine in amniotic fluid in prenatal diagnosis of MMA...
  100. ncbi The effect of yogurt on acetaminophen absorption by activated charcoal and burnt toast
    Amitava Dasgupta
    Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, Houston, TX 77030, USA
    J Clin Lab Anal 21:393-7. 2007
    ..was withdrawn at 0, 5, 10, 20, and 30 min and acetaminophen concentrations were measured by the fluorescence polarization immunoassay (FPIA)...
  101. ncbi Evaluation of the Abbott IMx fluorescence polarization immunoassay and the bio-rad enzyme immunoassay for homocysteine: comparison with high-performance liquid chromatography
    J G Donnelly
    Department of Laboratory Medicine, Ottawa Hospital, Ontario, Canada
    Ann Clin Biochem 37:194-8. 2000
    ..The IMx assay had superior precision as well as the technological advantage of being completely automated. Both immunoassays exhibited greatly improved throughput compared with our existing HPLC method...

Research Grants7

  1. Fluorescent HTS Assays for Human Sulfotransferases
    Robert Lowery; Fiscal Year: 2006
    ..we developed a homogenous, fluorescent high throughput screening (HTS) method called donor product fluorescence polarization immunoassay (DP-FPIA) that provides a universal screening platform for measuring sulfation of any compound by ..
  2. Fluorescent HTS Assay for Methyltransferases in Neurodegenerative Diseases
    Robert Lowery; Fiscal Year: 2006
    ..on detection of the invariable MT reaction product S-adenosyl homocysteine-using a competitive fluorescence polarization immunoassay. The Phase I work includes development of antibodies and fluorescent nucleotide conjugates that ..
  3. RHOMOCYCSTEINASE FOR HOMOCYSTEINE ASSAY
    Yuying Tan; Fiscal Year: 2002
    ..HPLC is highly specialized and low throughput, however. A fluorescence polarization immunoassay for tHCY has also been developed...
  4. Fluorescent HTS Assays for Methyltransferases in Neurodegenerative Diseases
    Robert Lowery; Fiscal Year: 2009
    ..To address this need, in Phase I BellBrook Labs developed a novel fluorescence polarization immunoassay for S-adenosylhomocysteine (SAH), the invariant product of all MT reactions...
  5. QUANTITATIVE IMAGING OF ZINC METABOLISM IN THE BRAIN
    Richard Thompson; Fiscal Year: 2002
    ..abstract_text> ..
  6. QUANTITATIVE IMAGING OF ZINC METABOLISM IN THE BRAIN
    Richard Thompson; Fiscal Year: 2003
    ..abstract_text> ..
  7. Fluorescence Zinc BioSensing and Imaging Technology
    RICHARD BLAIR THOMPSON; Fiscal Year: 2010
    ..This novel technology will provide the basis for new diagnostics and treatments. ..