fluorescent antibody technique

Summary

Summary: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.

Top Publications

  1. ncbi The small GTP-binding protein rac regulates growth factor-induced membrane ruffling
    A J Ridley
    Institute for Cancer Research, Chester Beatty Laboratories, London, England
    Cell 70:401-10. 1992
  2. pmc Identification and characterization of ovarian cancer-initiating cells from primary human tumors
    Shu Zhang
    Medical Sciences, Indiana University School of Medicine, Bloomington, Indiana 47405, USA
    Cancer Res 68:4311-20. 2008
  3. pmc Telocytes and putative stem cells in the lungs: electron microscopy, electron tomography and laser scanning microscopy
    Laurentiu M Popescu
    Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania
    Cell Tissue Res 345:391-403. 2011
  4. ncbi Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor
    Noboru Sato
    Laboratory of Molecular Vertebrate Embryology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Nat Med 10:55-63. 2004
  5. pmc Array tomography: a new tool for imaging the molecular architecture and ultrastructure of neural circuits
    Kristina D Micheva
    Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA
    Neuron 55:25-36. 2007
  6. ncbi Meiotic prophase arrest with failure of chromosome synapsis in mice deficient for Dmc1, a germline-specific RecA homolog
    D L Pittman
    Jackson Laboratory, Bar Harbor, Maine 04609, USA
    Mol Cell 1:697-705. 1998
  7. pmc Tau blocks traffic of organelles, neurofilaments, and APP vesicles in neurons and enhances oxidative stress
    K Stamer
    Max Planck Unit for Structural Molecular Biology, 22607 Hamburg, Germany
    J Cell Biol 156:1051-63. 2002
  8. pmc A major role for the Plasmodium falciparum ApiAP2 protein PfSIP2 in chromosome end biology
    Christian Flueck
    Department of Medical Parasitology and Infection Biology, Swiss Tropical Institute, University of Basel, Basel, Switzerland
    PLoS Pathog 6:e1000784. 2010
  9. ncbi The genomic response to retinal disease and injury: evidence for endothelin signaling from photoreceptors to glia
    Amir Rattner
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Neurosci 25:4540-9. 2005
  10. pmc An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition
    Claude C Warzecha
    Renal Division, Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    EMBO J 29:3286-300. 2010

Research Grants

  1. Inhibition of human neutrophil function by Francisella tularensis
    Lee Ann H Allen; Fiscal Year: 2012
  2. DNA Damage and Response in the Bladder Microenvironment.
    Bradley P Dixon; Fiscal Year: 2013
  3. Rac GTPase signaling during embryonic salivary gland branching morphogenesis
    Sharon Sequeira; Fiscal Year: 2012
  4. Axonopathy in Glaucoma
    Samuel D Crish; Fiscal Year: 2013
  5. Novel Peptide MPO Inhibitors for Treating Atherosclerosis
    Kirkwood A Pritchard; Fiscal Year: 2012
  6. Iron in the pathogenesis of Friedreich's ataxia
    ARNULF HANS WERNER KOEPPEN; Fiscal Year: 2013
  7. Cell signaling as a leukemia biomarker
    JAMES WILLIAM JACOBBERGER; Fiscal Year: 2013
  8. Nuclear-Cytoplasmic Shuttling of Foxc2 via 14-3-3 Determines Proximal Tubule Cell
    DIANA LYNNE GOLDEN; Fiscal Year: 2012
  9. Modifiers of Cyst Progression in Polycystic Kidney Disease
    Phillip Darwin Bell; Fiscal Year: 2013
  10. RSK-2 regulates integrin-mediated adhesion and migration
    JOE WILLIAM RAMOS; Fiscal Year: 2013

Detail Information

Publications417 found, 100 shown here

  1. ncbi The small GTP-binding protein rac regulates growth factor-induced membrane ruffling
    A J Ridley
    Institute for Cancer Research, Chester Beatty Laboratories, London, England
    Cell 70:401-10. 1992
    ..We propose that rac and rho are essential components of signal transduction pathways linking growth factors to the organization of polymerized actin...
  2. pmc Identification and characterization of ovarian cancer-initiating cells from primary human tumors
    Shu Zhang
    Medical Sciences, Indiana University School of Medicine, Bloomington, Indiana 47405, USA
    Cancer Res 68:4311-20. 2008
    ..Based on these findings, we assert that epithelial ovarian cancers derive from a subpopulation of CD44(+)CD117(+) cells, thus representing a possible therapeutic target for this devastating disease...
  3. pmc Telocytes and putative stem cells in the lungs: electron microscopy, electron tomography and laser scanning microscopy
    Laurentiu M Popescu
    Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania
    Cell Tissue Res 345:391-403. 2011
    ..In conclusion, this study shows the presence of TCs in lungs and identifies a TC-SC tandem in subepithelial niches of the bronchiolar tree. In TC-SCNs, the synergy of TCs and SCs may be based on nanocontacts and shed vesicles...
  4. ncbi Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor
    Noboru Sato
    Laboratory of Molecular Vertebrate Embryology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Nat Med 10:55-63. 2004
    ..These results suggest that the use of GSK-3-specific inhibitors such as BIO may have practical applications in regenerative medicine...
  5. pmc Array tomography: a new tool for imaging the molecular architecture and ultrastructure of neural circuits
    Kristina D Micheva
    Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA
    Neuron 55:25-36. 2007
    ..The application of array tomography can reveal important but previously unseen features of brain molecular architecture...
  6. ncbi Meiotic prophase arrest with failure of chromosome synapsis in mice deficient for Dmc1, a germline-specific RecA homolog
    D L Pittman
    Jackson Laboratory, Bar Harbor, Maine 04609, USA
    Mol Cell 1:697-705. 1998
    ..The strong similarity of phenotypes in Dmc1-deficient mice and yeast suggests that meiotic mechanisms have been highly conserved through evolution...
  7. pmc Tau blocks traffic of organelles, neurofilaments, and APP vesicles in neurons and enhances oxidative stress
    K Stamer
    Max Planck Unit for Structural Molecular Biology, 22607 Hamburg, Germany
    J Cell Biol 156:1051-63. 2002
    ..The data suggests a linkage between tau and APP trafficking, which may be significant in Alzheimer's disease...
  8. pmc A major role for the Plasmodium falciparum ApiAP2 protein PfSIP2 in chromosome end biology
    Christian Flueck
    Department of Medical Parasitology and Infection Biology, Swiss Tropical Institute, University of Basel, Basel, Switzerland
    PLoS Pathog 6:e1000784. 2010
    ..These findings are highly relevant for our understanding of chromosome end biology and variegated expression in P. falciparum and other eukaryotes, and for the future analysis of the role of ApiAP2-DNA interactions in parasite biology...
  9. ncbi The genomic response to retinal disease and injury: evidence for endothelin signaling from photoreceptors to glia
    Amir Rattner
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Neurosci 25:4540-9. 2005
    ....
  10. pmc An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition
    Claude C Warzecha
    Renal Division, Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    EMBO J 29:3286-300. 2010
    ....
  11. ncbi An essential role for beta-actin mRNA localization and translation in Ca2+-dependent growth cone guidance
    Jiaqi Yao
    Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, New Jersey 08854, USA
    Nat Neurosci 9:1265-73. 2006
    ....
  12. pmc Chikungunya virus-induced autophagy delays caspase-dependent cell death
    Pierre Emmanuel Joubert
    Unité Immunobiologie des Cellules Dendritiques, Department of Immunology, Institut Pasteur, Paris, France
    J Exp Med 209:1029-47. 2012
    ..Our study suggests that inducers of autophagy may limit the pathogenesis of acute Chikungunya disease...
  13. ncbi Proteomic analysis of rhoptry organelles reveals many novel constituents for host-parasite interactions in Toxoplasma gondii
    Peter J Bradley
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305, USA
    J Biol Chem 280:34245-58. 2005
    ....
  14. pmc The nuclear protein HMGB1 is secreted by monocytes via a non-classical, vesicle-mediated secretory pathway
    Stefania Gardella
    National Cancer Research Institute, Genoa, Italy
    EMBO Rep 3:995-1001. 2002
    ..Thus, in monocytes, non-classical secretion can occur through vescicle compartments that are at least partially distinct...
  15. pmc Staphylococcus aureus-derived peptidoglycan induces Cx43 expression and functional gap junction intercellular communication in microglia
    Sarita Garg
    Department of Neurobiology and Developmental Sciences, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    J Neurochem 95:475-83. 2005
    ....
  16. pmc Nuclear localization and regulation of erk- and rsk-encoded protein kinases
    R H Chen
    Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, Massachusetts 02115
    Mol Cell Biol 12:915-27. 1992
    ....
  17. doi FGF9 suppresses meiosis and promotes male germ cell fate in mice
    Josephine Bowles
    Division of Molecular Genetics and Development, Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia
    Dev Cell 19:440-9. 2010
    ....
  18. pmc Characterization of a cis-Golgi matrix protein, GM130
    N Nakamura
    Cell Biology Laboratory, Imperial Cancer Research Fund, London, UK
    J Cell Biol 131:1715-26. 1995
    ..Together these results suggest that GM130 is part of a cis-Golgi matrix and has a role in maintaining cis-Golgi structure...
  19. ncbi Transient expression of doublecortin during adult neurogenesis
    Jason P Brown
    Department of Neurology, University of Regensburg, 93053 Regensburg, Germany
    J Comp Neurol 467:1-10. 2003
    ..This assumption is further supported by our observation that the number of DCX-expressing cells in the dentate gyrus was decreased with age according to the reduction of neurogenesis in the aging dentate gyrus previously reported...
  20. ncbi Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3
    H Saitoh
    The Picower Institute for Medical Research, Manhasset, New York 11030, USA
    J Biol Chem 275:6252-8. 2000
    ..Together, these results support the concept of important distinctions between the SUMO-2/3 and SUMO-1 conjugation pathways and suggest a role for SUMO-2/3 in the cellular responses to environmental stress...
  21. ncbi Directed differentiation of telencephalic precursors from embryonic stem cells
    Kiichi Watanabe
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe 650 0047, Japan
    Nat Neurosci 8:288-96. 2005
    ..Thus, in the absence of caudalizing signals, floating aggregates of ES cells generate naive telencephalic precursors that acquire subregional identities by responding to extracellular patterning signals...
  22. ncbi Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus
    X D Fu
    Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138
    Nature 343:437-41. 1990
    ..These observations are evidence that spliceosome assembly could be compartmentalized within the nucleus...
  23. pmc The Nup107-160 complex and gamma-TuRC regulate microtubule polymerization at kinetochores
    Ram Kumar Mishra
    Laboratory of Gene Regulation and Development, NICHD NIH, Bethesda, MD 20892, USA
    Nat Cell Biol 12:164-9. 2010
    ....
  24. pmc Export of a Toxoplasma gondii rhoptry neck protein complex at the host cell membrane to form the moving junction during invasion
    Sebastien Besteiro
    UMR 5235 CNRS, Université de Montpellier 2, Montpelier, France
    PLoS Pathog 5:e1000309. 2009
    ..Globally, these results point toward a model of MJ organization where the parasite would be secreting and inserting interacting components on either side of the MJ, both at the host and at its own plasma membranes...
  25. ncbi Aggregation of huntingtin in neuronal intranuclear inclusions and dystrophic neurites in brain
    M Difiglia
    Department of Neurology, Massachusetts General Hospital, Boston, MA 02114, USA
    Science 277:1990-3. 1997
    ..Ubiquitin was also found in NIIs and DNs, which suggests that abnormal huntingtin is targeted for proteolysis but is resistant to removal. The aggregation of mutant huntingtin may be part of the pathogenic mechanism in HD...
  26. ncbi Recurrent de novo point mutations in lamin A cause Hutchinson-Gilford progeria syndrome
    Maria Eriksson
    Department of Human Genetics, New York State Institute for Basic Research in Developmental Disabilities, Staten Island, New York 10314, USA
    Nature 423:293-8. 2003
    ..The discovery of the molecular basis of this disease may shed light on the general phenomenon of human ageing...
  27. ncbi mDia mediates Rho-regulated formation and orientation of stable microtubules
    A F Palazzo
    Department of Anatomy and Cell Biology, Columbia University, New York 10032, USA
    Nat Cell Biol 3:723-9. 2001
    ..Our results show that mDia is sufficient to generate and orient stable microtubules, and indicate that Dia-related formins are part of a conserved pathway that regulates the dynamics of microtubule ends...
  28. ncbi Localization of organellar proteins in Plasmodium falciparum using a novel set of transfection vectors and a new immunofluorescence fixation method
    Christopher J Tonkin
    Plant Cell Biology Research Centre, School of Botany, The University of Melbourne, Parkville, VIC 3010, Australia
    Mol Biochem Parasitol 137:13-21. 2004
    ..falciparum proteins...
  29. pmc Signal recognition particle components in the nucleolus
    J C Politz
    Department of Biochemistry, University of Massachusetts Medical School, 377 Plantation Street, Suite 337, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 97:55-60. 2000
    ..Our findings support the notion that the nucleolus is the site of assembly and/or interaction between the family of ribonucleoproteins involved in protein synthesis, in addition to ribosomes themselves...
  30. pmc Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line
    J V Kilmartin
    J Cell Biol 93:576-82. 1982
    ..The purified antibodies could be conjugated to colloidal gold particles and used for direct labeling of yeast microtubules for electron microscopy...
  31. pmc Focal adhesion disassembly requires clathrin-dependent endocytosis of integrins
    Wei Ting Chao
    Department of Molecular Physiology and Biophysics, Baylor College of Medicine, 1 Baylor Plaza, BCM335, RM T419, Houston, TX 77030, USA
    FEBS Lett 583:1337-43. 2009
    ..Together, these results provide important insight into the mechanisms underlying adhesion disassembly and identify novel components of the disassembly pathway...
  32. pmc Mandibuloacral dysplasia is caused by a mutation in LMNA-encoding lamin A/C
    Giuseppe Novelli
    Department of Biopathology and Diagnostic Imaging, Faculty for Medicine and Surgery, University of Rome Tor Vergata, Via Montpellier 1, 00133 Romea, Italy
    Am J Hum Genet 71:426-31. 2002
    ..Patient skin fibroblasts showed nuclei that presented abnormal lamin A/C distribution and a dysmorphic envelope, thus demonstrating the pathogenic effect of the R527H LMNA mutation...
  33. pmc Glycogen synthase kinase-3beta regulates cyclin D1 proteolysis and subcellular localization
    J A Diehl
    Howard Hughes Medical Institute, St Jude Children s Research Hospital, Memphis, Tennessee 38105 USA
    Genes Dev 12:3499-511. 1998
    ..Therefore, phosphorylation and proteolytic turnover of cyclin D1 and its subcellular localization during the cell division cycle are linked through the action of GSK-3beta...
  34. pmc Neuregulin-1 type III determines the ensheathment fate of axons
    Carla Taveggia
    Department of Cell Biology, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, New York 10016, USA
    Neuron 47:681-94. 2005
    ..These results indicate that levels of NRG1 type III, independent of axon diameter, provide a key instructive signal that determines the ensheathment fate of axons...
  35. doi Antigenicity and immunogenicity of a novel chimeric peptide antigen based on the P. vivax circumsporozoite protein
    Nora Céspedes
    Malaria Vaccine and Drug Development Center MVDC, Carrera 37 2Bis No 5E 08, A A 25574 Cali, Colombia Biochemistry Department, University of Lausanne, 155 ch des Boveresses, 1066 Epalinges, Lausanne, Switzerland Faculty of Health, University of Valle, Calle 4B No 36 00 Cali, Colombia
    Vaccine 31:4923-30. 2013
    ..PvCS contains a dimorphic central repetitive fragment flanked by conserved regions that contain functional domains...
  36. ncbi Mouse very long-chain Acyl-CoA synthetase 3/fatty acid transport protein 3 catalyzes fatty acid activation but not fatty acid transport in MA-10 cells
    Zhengtong Pei
    Kennedy Krieger Research Institute and Department of Neurology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 279:54454-62. 2004
    ..These studies cast doubt on the designation of ACSVL3 as a fatty acid transport protein...
  37. ncbi Production of melanin by Aspergillus fumigatus
    Sirida Youngchim
    Dermatology Department, St Johns Institute of Dermatology, Guy s Hospital, Kings and St Thomas Medical Schools, London, UK
    J Med Microbiol 53:175-81. 2004
    ..Melanin-binding mAbs were used to detect the presence of melanized conidia in three patients with nasal aspergilloma, indicating that in vivo melanization may occur during infection...
  38. ncbi CD133, a novel marker for human prostatic epithelial stem cells
    Gavin D Richardson
    Prostate Research Group, Surgical Oncology, Medical School, Framlington Place, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, UK
    J Cell Sci 117:3539-45. 2004
    ..alpha(2)beta(1)(hi)/CD133(+) cells exhibit two important attributes of epithelial stem cells: they possess a high in vitro proliferative potential and can reconstitute prostatic-like acini in immunocompromised male nude mice...
  39. pmc Evidence for transcriptional activity in the syncytiotrophoblast of the human placenta
    P M Ellery
    Centre for Trophoblast Research, University of Cambridge, Downing Street, Cambridge CB2 3EG, UK
    Placenta 30:329-34. 2009
    ..Contrary to previous findings, these results confirm that a proportion of syncytiotrophoblast nuclei actively produce mRNA transcripts...
  40. ncbi The coxsackie- and adenovirus receptor (CAR) is an in vivo marker for epithelial tight junctions, with a potential role in regulating permeability and tissue homeostasis
    Elisabeth Raschperger
    Ludwig Institute for Cancer Research, Nobels v 3, SE 171 77 Stockholm, Sweden
    Exp Cell Res 312:1566-80. 2006
    ..We conclude that CAR is localized to epithelial tight junctions in vivo where it may play a role in the regulation of epithelial permeability and tissue homeostasis...
  41. doi Early exercise promotes positive hippocampal plasticity and improves spatial memory in the adult life of rats
    Sergio Gomes da Silva
    Department of Physiology, Universidade Federal de Sao Paulo, Sao Paulo, Brazil
    Hippocampus 22:347-58. 2012
    ..Our findings demonstrate that physical exercise during postnatal development results in positive changes for the hippocampal formation, both in structure and function...
  42. doi mTORC1 activation triggers the unfolded protein response in podocytes and leads to nephrotic syndrome
    Noriko Ito
    Department of Pediatrics, Kyorin University School of Medicine, Mitaka, Tokyo, Japan
    Lab Invest 91:1584-95. 2011
    ..In conclusion, mTORC1 activation may perturb the regulatory system of energy metabolism primarily by promoting energy consumption and inducing the UPR, which underlie proteinuria in MCD...
  43. pmc RNA interference targeting survivin exerts antitumoral effects in vitro and in established glioma xenografts in vivo
    Sandy Hendruschk
    Department of Neurosurgery, University Hospital Carl Gustav Carus, Dresden, Germany
    Neuro Oncol 13:1074-89. 2011
    ..We conclude that survivin is functionally relevant in gliomas and that PEI-mediated exogenous delivery of siRNA targeting survivin is a promising strategy for glioblastoma therapy...
  44. pmc Premature osteoblast clustering by enamel matrix proteins induces osteoblast differentiation through up-regulation of connexin 43 and N-cadherin
    Richard J Miron
    Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland
    PLoS ONE 6:e23375. 2011
    ..These findings provide further support for the clinical evidence that EMD increases the speed and quality of new bone formation in vivo...
  45. pmc Chk2 deficiency in Myc overexpressing lymphoma cells elicits a synergistic lethal response in combination with PARP inhibition
    Andreas Höglund
    Department of Molecular Biology, Umea University, Umea, Sweden
    Cell Cycle 10:3598-607. 2011
    ..Our data indicates that only certain types of chemotherapy would give rise to a synergistic lethal response in combination with specific Chk2 inhibitors, which will be important if Chk2 inhibitors enter the clinic...
  46. pmc CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis
    H Liao
    Fox Chase Cancer Center, Institute for Cancer Research, Philadelphia, Pennsylvania 19111, USA
    J Cell Biol 130:507-18. 1995
    ..The structural features deduced from our sequence studies and the spatial and temperal distribution of CENP-F revealed in our cytological and biochemical studies suggest that it may play a role in several mitotic events...
  47. doi Onecut transcription factors are required for the second phase of development of the A13 dopaminergic nucleus in the mouse
    Agnès Espana
    Universite Catholique de Louvain, Institute of Neuroscience, Laboratory of Neural Differentiation, Brussels, B 1200, Belgium
    J Comp Neurol 520:1424-41. 2012
    ..Hence, Onecut factors are markers of the A13 nucleus throughout embryonic development. They are dispensable for the first phase of A13 development but are required for the second phase of development and for maintenance of this nucleus...
  48. doi Kif7 promotes hedgehog signaling in growth plate chondrocytes by restricting the inhibitory function of Sufu
    Shu Hsuan C Hsu
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children, Toronto, Ontario, Canada
    Development 138:3791-801. 2011
    ..Furthermore, Kif7 also inhibits Gli transcriptional activity in the chondrocytes when Sufu function is absent. Therefore, Kif7 regulates the activity of Gli transcription factors through both Sufu-dependent and -independent mechanisms...
  49. ncbi An essential cytoskeletal linker protein connecting actin microfilaments to intermediate filaments
    Y Yang
    Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, The University of Chicago, Illinois 60637, USA
    Cell 86:655-65. 1996
    ..In BPAG1 null mice, axonal architecture is markedly perturbed, consistent with a failure to tether neurofilaments to the actin cytoskeleton and underscoring the physiological relevance of this protein...
  50. doi Intracerebroventricular infusion of monoclonal antibody or its derived Fab fragment against misfolded forms of SOD1 mutant delays mortality in a mouse model of ALS
    Francois Gros-Louis
    Department of Psychiatry and Neuroscience, Research Centre of CHUQ, Universite Laval, Quebec, QC, Canada
    J Neurochem 113:1188-99. 2010
    ..From these results, we propose that passive immunization strategies should be considered as potential avenues for treatment of familial amyotrophic lateral sclerosis caused by SOD1 mutations...
  51. doi Feeder- and serum-free establishment and expansion of human induced pluripotent stem cells
    Mehdi Totonchi
    Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Int J Dev Biol 54:877-86. 2010
    ..Such hiPSCs could be useful particularly in the context of in vitro disease modeling, pharmaceutical screening and in cellular replacement therapies once the safety issues have been overcome...
  52. pmc Epigenetic modifications on X chromosomes in marsupial and monotreme mammals and implications for evolution of dosage compensation
    Willem Rens
    Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 OES, United Kingdom
    Proc Natl Acad Sci U S A 107:17657-62. 2010
    ..In comparison to the marsupial, the monotreme exhibited no epigenetic differences between homologous X chromosomes, suggesting the absence of a dosage compensation process comparable to that in therians...
  53. ncbi Control of autofluorescence of archival formaldehyde-fixed, paraffin-embedded tissue in confocal laser scanning microscopy (CLSM)
    W Baschong
    M E Muller Institute at the Biozentrum, Aarau, Switzerland
    J Histochem Cytochem 49:1565-72. 2001
    ..Sudan Black B is suggested to mask fluorescent tissue components...
  54. ncbi The mago nashi locus encodes an essential product required for germ plasm assembly in Drosophila
    P A Newmark
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309
    Development 120:1303-13. 1994
    ..These results demonstrate that mago nashi encodes an essential product necessary for the localization of germ plasm components to the posterior pole of the oocyte...
  55. doi The polo-like kinase 1 regulates CDC25B-dependent mitosis entry
    Valerie Lobjois
    Universite de Toulouse, LBCMCP, 118 route de Narbonne, CNRS, LBCMCP UMR5088, F 31062 Toulouse, France
    Biochim Biophys Acta 1793:462-8. 2009
    ..Our results support a model in which the relocalisation of CDC25B to the nucleus at the G2-M transition by PLK1 regulates its mitotic inducing activity...
  56. pmc Analysis of endoplasmic reticulum trafficking signals by combinatorial screening in mammalian cells
    N Zerangue
    Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco, CA 94143-0725, USA
    Proc Natl Acad Sci U S A 98:2431-6. 2001
    ..Finally, using sequences from our screen, we found that the potency of di-lysine, but not di-arginine, mediated endoplasmic reticulum localization was correlated with the strength of interaction with alpha-COP...
  57. ncbi Tissue-specific and inducible Cre-mediated recombination in the gut epithelium
    Fatima el Marjou
    Morphogenesis and intracellular signalling, Institut Curie CNRS, Paris, France
    Genesis 39:186-93. 2004
    ..The villin-Cre and villin-Cre-ERT2 mice provide valuable tools for studies of cell lineage allocation and gene function in the developing and adult intestine...
  58. pmc CXCL16 is expressed in podocytes and acts as a scavenger receptor for oxidized low-density lipoprotein
    Paul Gutwein
    Pharmazentrum Frankfurt, Klinikum der Johann Wolfgang Goethe Universitat Frankfurt, Theodor Stern Kai 7, D 60590 Frankfurt am Main, Germany
    Am J Pathol 174:2061-72. 2009
    ..In summary, we presume important roles for CXCL16, ADAM10, and ADAM17 in the development of MN, suggesting these proteins as new therapeutic targets in this glomerular kidney disease...
  59. ncbi AnkyrinG. A new ankyrin gene with neural-specific isoforms localized at the axonal initial segment and node of Ranvier
    E Kordeli
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710
    J Biol Chem 270:2352-9. 1995
    ..The neural-specific ankyrinG polypeptides are candidates to participate in maintenance/targeting of ion channels and cell adhesion molecules to nodes of Ranvier and axonal initial segments...
  60. pmc Knockdown of Rab5a expression decreases cancer cell motility and invasion through integrin-mediated signaling pathway
    Shan Shan Liu
    Department of Life Science and Engineering, Harbin Institute of Technology, Harbin 150001, PR China
    J Biomed Sci 18:58. 2011
    ..In the present study, we investigated the effect of Rab5a on cervical cancer invasion and metastasis and the molecular mechanism underlying the involvement of Rab5a...
  61. pmc Primary cilia disappear in rat podocytes during glomerular development
    Koichiro Ichimura
    Department of Anatomy and Life Structure, Juntendo University School of Medicine, Tokyo, Japan
    Cell Tissue Res 341:197-209. 2010
    ..Thus, the disappearance of the primary cilia presumably prevents the entry of excessive calcium-ions via the cilium-associated polycystin complexes and the disturbance of intracellular signaling cascades in mature podocytes...
  62. doi SUMO1 modification of PTEN regulates tumorigenesis by controlling its association with the plasma membrane
    Jian Huang
    Department of Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory of Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine SJTU SM, Shanghai 200025, China
    Nat Commun 3:911. 2012
    ..Our data demonstrate a molecular mechanism whereby SUMO1 modification is required for PTEN tumour suppressor function by controlling PTEN membrane association and regulation of the phosphatidylinositol-3 kinase/AKT pathway...
  63. ncbi C-kit immunopositive interstitial cells (Cajal-type) in human myometrium
    Sanda M Ciontea
    Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, 050474, Romania
    J Cell Mol Med 9:407-20. 2005
    ..In conclusion, we describe myometrial Cajal-like interstitial cells that have affinity for methylene blue and Janus green B vital dyes, fulfill (all) TEM criteria, express CD117/c-kit and have spontaneous electric activity...
  64. ncbi Bipolar, Dual Plasmodium falciparum helicase 45 expressed in the intraerythrocytic developmental cycle is required for parasite growth
    Arun Pradhan
    Malaria Group, International Centre for Genetic Engineering and Biotechnology, PO Box 10504, Aruna Asaf Ali Marg, New Delhi 110067, India
    J Mol Biol 373:268-81. 2007
    ..These studies indicate that PfH45 is an indispensable enzyme that is essential for growth, and probably survival, of P. falciparum...
  65. ncbi EMMPRIN-induced MMP-2 activation cascade in human cervical squamous cell carcinoma
    Cornelis F M Sier
    Department of Pathology, Leiden University Medical Center, The Netherlands
    Int J Cancer 118:2991-8. 2006
    ....
  66. pmc Synaptic vesicle-like lipidome of human cytomegalovirus virions reveals a role for SNARE machinery in virion egress
    Sean T H Liu
    Department of Molecular Biology, Lewis Thomas Laboratory, University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 108:12869-74. 2011
    ..We propose that cytomegalovirus capsids acquire their envelope by budding into vesicles with a lipid composition similar to that of synaptic vesicles, which subsequently fuse with the plasma membrane to release virions from the cell...
  67. pmc The kinetically dominant assembly pathway for centrosomal asters in Caenorhabditis elegans is gamma-tubulin dependent
    Eva Hannak
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    J Cell Biol 157:591-602. 2002
    ..Thus, although unknown mechanisms can support partial assembly of mitotic centrosomal asters, gamma-tubulin is the kinetically dominant centrosomal MT nucleator...
  68. ncbi TWEAK-Fn14 pathway inhibition protects the integrity of the neurovascular unit during cerebral ischemia
    Xiaohui Zhang
    Department of Neurology and Center for Neurodegenerative Disease, Emory University School of Medicine, Atlanta, GA 30322, USA
    J Cereb Blood Flow Metab 27:534-44. 2007
    ..These findings show that the cytokine TWEAK plays a role in the disruption of the structure of the NVU during cerebral ischemia and that TWEAK antagonism is a potential therapeutic strategy for acute cerebral ischemia...
  69. pmc Lis1 is essential for cortical microtubule organization and desmosome stability in the epidermis
    Kaelyn D Sumigray
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    J Cell Biol 194:631-42. 2011
    ..This work reveals essential desmosome-associated components that control cortical microtubule organization and unexpected roles for centrosomal proteins in epidermal function...
  70. pmc Wiskott-Aldrich syndrome protein regulates podosomes in primary human macrophages
    S Linder
    Max von Pettenkofer Institut für Medizinische Mikrobiologie, Pettenkoferstrasse 9a, Ludwig Maximilians Universitat, 80336 Munich, Germany
    Proc Natl Acad Sci U S A 96:9648-53. 1999
    ..These findings indicate that WASp controls podosome assembly and, in cooperation with CDC42Hs, podosome disassembly in primary human macrophages...
  71. pmc An epidemiological study of RSV infection in the Gambia
    Martin W Weber
    Medical Research Council Laboratories, Fajara, The Gambia
    Bull World Health Organ 80:562-8. 2002
    ..To describe the epidemiology of respiratory syncytial virus (RSV) infection in a developing country...
  72. pmc Versatile fluorescent probes for actin filaments based on the actin-binding domain of utrophin
    Brian M Burkel
    Department of Zoology, University of Wisconsin Madison, Madison, Wisconsin, USA
    Cell Motil Cytoskeleton 64:822-32. 2007
    ....
  73. ncbi Phase segregation of medial septal GABAergic neurons during hippocampal theta activity
    Zsolt Borhegyi
    Department of Cellular and Network Neurobiology, Institute of Experimental Medicine of the Hungarian Academy of Science, Budapest 1083, Hungary
    J Neurosci 24:8470-9. 2004
    ....
  74. pmc Gene regulation logic in retinal ganglion cell development: Isl1 defines a critical branch distinct from but overlapping with Pou4f2
    Xiuqian Mu
    Department of Biochemistry and Molecular Biology, M D Anderson Cancer Center and Graduate Training Program in Genes and Development, Graduate School of Biomedical Sciences, University of Texas, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 105:6942-7. 2008
    ..They also reveal that identical RGC expression patterns are achieved by different combinations of divergent inputs from upstream transcription factors...
  75. ncbi DNA replication licensing and human cell proliferation
    K Stoeber
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
    J Cell Sci 114:2027-41. 2001
    ..We conclude that biomarkers for replication-licensed cells detect, in addition to actively proliferating cells, cells with growth potential, a concept that has major implications for developmental and cancer biology...
  76. pmc Nucleolar assembly of the rRNA processing machinery in living cells
    T M Savino
    Institut Jacques Monod, UMR 7592, 2 place Jussieu, 75251 Paris Cedex 05, France
    J Cell Biol 153:1097-110. 2001
    ..This demonstrates the formation of one functional domain by cooperative interactions between different chromosome territories...
  77. pmc A bicomponent Plasmodium falciparum investigational vaccine composed of protein-peptide conjugates
    Joanna Kubler-Kielb
    Program on Developmental and Molecular Immunity, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 107:1172-7. 2010
    ....
  78. pmc Compartmentalized organization: a common and required feature of stem cell niches?
    Valentina Greco
    Department of Genetics, Yale University School of Medicine and Yale Stem Cell Center, SHM I 141A, New Haven, CT 06510, USA
    Development 137:1586-94. 2010
    ....
  79. pmc Detection of Leishmania infantum in dogs by PCR with lymph node aspirates and blood
    S Reale
    Istituto Zooprofilattico Sperimentale della Sicilia, Palermo, Italy
    J Clin Microbiol 37:2931-5. 1999
    ..These animals could contribute to the spreading of infection among dogs, and represent a potential risk for human health as well...
  80. doi Quantitative analysis of changes in ER, PR and HER2 expression in primary breast cancer and paired nodal metastases
    S J Aitken
    Division of Pathology, University of Edinburgh, Edinburgh, UK
    Ann Oncol 21:1254-61. 2010
    ..Since de novo resistance to therapy is common, we quantified differences in receptor expression between primary and nodal disease in order to assess whether this might contribute to therapeutic resistance...
  81. pmc CIP2A expression and localization in oral carcinoma and dysplasia
    Joseph Katz
    Department of Oral Maxillofacial Surgery and Diagnostic Sciences, University of Florida, College of Dentistry, FL, USA
    Cancer Biol Ther 10:694-9. 2010
    ..This study was designed to investigate the expression of CIP2A in OSCC cell lines and tissues representing human normal, dysplasia and OSCC...
  82. pmc Human cytomegalovirus UL29/28 protein interacts with components of the NuRD complex which promote accumulation of immediate-early RNA
    Scott S Terhune
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
    PLoS Pathog 6:e1000965. 2010
    ..We propose that pUL29/28 modifies the NuRD complex to stimulate the accumulation of immediate-early RNAs...
  83. pmc Symplekin promotes tumorigenicity by up-regulating claudin-2 expression
    Michael Buchert
    Institut de Genomique Fonctionnelle, Centre National de la Recherche Scientifique, Unite Mixte de Recherche 5203, Institut National de la Sante et de la Recherche Medicale U661, and Université Montpellier 1 and 2, Montpellier F 34094, France
    Proc Natl Acad Sci U S A 107:2628-33. 2010
    ..Our results suggest that nuclear overexpression of symplekin promotes tumorigenesis in the human colon and that the regulation of claudin-2 expression is instrumental in this effect...
  84. doi In vivo formation of gamma-H2AX and 53BP1 DNA repair foci in blood cells after radioiodine therapy of differentiated thyroid cancer
    Michael Lassmann
    Department of Nuclear Medicine, University of Wurzburg, Wurzburg, Germany
    J Nucl Med 51:1318-25. 2010
    ....
  85. ncbi Sequential assembly of the nucleotide excision repair factors in vivo
    M Volker
    Department of Radiation Genetics and Chemical Mutagenesis, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL, Leiden, The Netherlands
    Mol Cell 8:213-24. 2001
    ....
  86. pmc Characterization of a Drosophila centrosome protein CP309 that shares homology with Kendrin and CG-NAP
    Shin Ichi Kawaguchi
    Department of Embryology, Carnegie Institution of Washington and Howard Hughes Medical Institute, Baltimore, Maryland 21210, USA
    Mol Biol Cell 15:37-45. 2004
    ..These findings suggest that the microtubule-nucleating activity of the centrosome requires the function of CP309...
  87. pmc Increased disease severity of parasite-infected TLR2-/- mice is correlated with decreased central nervous system inflammation and reduced numbers of cells with alternatively activated macrophage phenotypes in a murine model of neurocysticercosis
    Uma Mahesh Gundra
    Department of Biology, South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, One UTSA Circle, San Antonio, Texas 78249 1644, USA
    Infect Immun 79:2586-96. 2011
    ..Importantly, these results demonstrate that TLR2-associated responses modulate the disease severity of murine NCC...
  88. pmc N-cadherin is dispensable for pancreas development but required for beta-cell granule turnover
    Jenny K Johansson
    Stem Cell Center, Department of Laboratory Medicine, Lund University, Sweden
    Genesis 48:374-81. 2010
    ..The number of insulin secretory granules is significantly reduced in N-cadherin-deficient beta-cells, and as a consequence insulin secretion is decreased...
  89. pmc Human airway epithelial cell culture to identify new respiratory viruses: coronavirus NL63 as a model
    Bridget S Banach
    Department of Microbiology and Immunology, Loyola University Chicago Stritch School of Medicine, 2160 South First Avenue, Maywood, IL, USA
    J Virol Methods 156:19-26. 2009
    ....
  90. ncbi Expression and localisation of GLUT1 and GLUT12 glucose transporters in the pregnant and lactating rat mammary gland
    Maria L Macheda
    Department of Medicine, St Vincent s Hospital, University of Melbourne, PO Box 2900, Fitzroy, Victoria 3065, Australia
    Cell Tissue Res 311:91-7. 2003
    ..Forced weaning resulted in decreased cytoplasmic GLUT1 staining intensity, but no change in GLUT12 staining. The results suggest a possible role for GLUT12 in the metabolism of mammary epithelial cells during pregnancy and lactation...
  91. ncbi Lipopolysaccharide O antigen chains mask IcsA (VirG) in Shigella flexneri
    Renato Morona
    Department of Molecular and Biomedical Science, University of Adelaide, SA, Australia
    FEMS Microbiol Lett 221:173-80. 2003
    ..These results have implications for the mechanism of IcsA export. They suggest that while IcsA export is predominantly targeted to the old cell pole, it can also occur on the lateral regions of the cell surface...
  92. ncbi The making of a gradient: IcsA (VirG) polarity in Shigella flexneri
    J R Robbins
    Department of Biochemistry, Stanford University School of Medicine, 279 West Campus Drive, Stanford, CA 94305-5307, USA
    Mol Microbiol 41:861-72. 2001
    ....
  93. pmc N-WASp is required for Schwann cell cytoskeletal dynamics, normal myelin gene expression and peripheral nerve myelination
    Fuzi Jin
    Department of Haematology, Xijing Hospital, Fourth Military Medical University, Xi an, China
    Development 138:1329-37. 2011
    ..Our observations demonstrate that N-WASp plays an essential role in Schwann cell maturation and myelin formation...
  94. ncbi Implications of cytokeratin 8/18 filament formation in stratified epithelial cells: induction of transformed phenotype
    Ujwala Raul
    Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Cancer Research Institute, Kharghar, Navi Mumbai, India
    Int J Cancer 111:662-8. 2004
    ..In both cases, lung metastasis was also seen. Thus, in conclusion, it appears that increased expression of CK8 in some way changes the phenotypic characteristics of stratified epithelial cells, resulting in malignant transformation...
  95. pmc Myt1 and Ngn3 form a feed-forward expression loop to promote endocrine islet cell differentiation
    Sui Wang
    Program in Developmental Biology, Department of Cell and Developmental Biology, 465 21st Avenue South, Rm 4128, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Dev Biol 317:531-40. 2008
    ....
  96. ncbi Angiotensin AT2 receptor protects against cerebral ischemia-induced neuronal injury
    Jun Li
    Center for Cardiovascular Research Institute of Pharmacology and Toxicology, Charite University Medicine Berlin, Germany
    FASEB J 19:617-9. 2005
    ..Our data indicate that cerebral AT2 receptors exert neuroprotective actions in response to ischemia-induced neuronal injury, possibly by supporting neuronal survival and neurite outgrowth in peri-ischemic brain areas...
  97. pmc Evidence for a lack of DNA double-strand break repair in human cells exposed to very low x-ray doses
    Kai Rothkamm
    Fachrichtung Biophysik, Universitat des Saarlandes, 66421 Homburg, Germany
    Proc Natl Acad Sci U S A 100:5057-62. 2003
    ....
  98. pmc Ubiquitin ligase cullin 7 induces epithelial-mesenchymal transition in human choriocarcinoma cells
    Jiejun Fu
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
    J Biol Chem 285:10870-9. 2010
    ..These results suggest that the CUL7 E3 ligase is a key regulator in trophoblast cell epithelial-mesenchymal transition and placental development...
  99. pmc Serum-dependent selective expression of EhTMKB1-9, a member of Entamoeba histolytica B1 family of transmembrane kinases
    Shiteshu Shrimal
    School of Life Sciences, Jawaharlal Nehru University, New Delhi, India
    PLoS Pathog 6:e1000929. 2010
    ..Our results suggest that E. histolytica TMKs of B1 family are functional kinases likely to be involved in serum response and cellular proliferation...
  100. pmc Aldehyde dehydrogenase-expressing colon stem cells contribute to tumorigenesis in the transition from colitis to cancer
    Joseph E Carpentino
    McKnight Brain Institute, University of Florida, Gainesville, Florida 32609, USA
    Cancer Res 69:8208-15. 2009
    ..These data link colitis and cancer identifying potential tumor-initiating cells from colitic patients, suggesting that sphere and/or xenograft formation will be useful to survey colitic patients at risk of developing cancer...
  101. ncbi Expression and modulation of an NADPH oxidase in mammalian astrocytes
    Andrey Y Abramov
    Department of Physiology, University College London, London WC1E 6BT, United Kingdom
    J Neurosci 25:9176-84. 2005
    ..The astrocytic NADPH oxidase is likely to play important roles in CNS physiology and pathology...

Research Grants71

  1. Inhibition of human neutrophil function by Francisella tularensis
    Lee Ann H Allen; Fiscal Year: 2012
    ..The results of this study may lead to new treatments to combat this severe and often fatal lung infection. ..
  2. DNA Damage and Response in the Bladder Microenvironment.
    Bradley P Dixon; Fiscal Year: 2013
    ..This may lead to cancer of the bladder, especially in children who have their bladders surgically reconstructed. ..
  3. Rac GTPase signaling during embryonic salivary gland branching morphogenesis
    Sharon Sequeira; Fiscal Year: 2012
    ....
  4. Axonopathy in Glaucoma
    Samuel D Crish; Fiscal Year: 2013
    ..This proposal is designed with the goal of identifying new therapeutic targets for this disease as well as identifying the potential role that function-restoring therapies could play in glaucoma and other neurodegenerative diseases. ..
  5. Novel Peptide MPO Inhibitors for Treating Atherosclerosis
    Kirkwood A Pritchard; Fiscal Year: 2012
    ..As these disease states represent a major focus of the NIH, findings from our proposal will advance the research and mission of several different institutes. ..
  6. Iron in the pathogenesis of Friedreich's ataxia
    ARNULF HANS WERNER KOEPPEN; Fiscal Year: 2013
    ..The work is clinically relevant because it will resolve questions about iron in the formal pathogenesis and natural history of FRDA, and the potential value of iron chelation. ..
  7. Cell signaling as a leukemia biomarker
    JAMES WILLIAM JACOBBERGER; Fiscal Year: 2013
    ....
  8. Nuclear-Cytoplasmic Shuttling of Foxc2 via 14-3-3 Determines Proximal Tubule Cell
    DIANA LYNNE GOLDEN; Fiscal Year: 2012
    ..Extensive examination of the mechanisms and potential therapeutic targets will advance the understanding and potential cure for AKI in proximal tubular regeneration. ..
  9. Modifiers of Cyst Progression in Polycystic Kidney Disease
    Phillip Darwin Bell; Fiscal Year: 2013
    ..Thus there is a compelling reason to focus on this disease and its effective treatment or cure, which would greatly benefit the VA patient population. ..
  10. RSK-2 regulates integrin-mediated adhesion and migration
    JOE WILLIAM RAMOS; Fiscal Year: 2013
    ..This will determine if these proteins are suitable targets for the development of new adhesion specific disease therapies. In particular we will test if RSK2 can serve as a potential new therapeutic target in glioma. ..
  11. Role of murine induced pluripotent stem cells on the correction of cardiac and sk
    Diego Fraidenraich; Fiscal Year: 2010
    ..These experiments will help elucidate the molecular mechanisms that the iPS cells may utilize to effect corrections in cardiac and skeletal muscle and will broaden the therapeutic applicability of the iPS cells. ..
  12. AKAP Regulation of PKA Targeting in the Heart
    Meredith Bond; Fiscal Year: 2009
    ..Our project investigates a novel nuclear AKAP protein, Chd8 that may participate in regulation of cardiac remodeling during ischemic heart failure. ..
  13. Connexins in Neuronal and Glial Gap Junctions in the Central Nervous System
    John E Rash; Fiscal Year: 2012
    ..normal human cerebral cortex and hippocampus. These data will serve as a prelude to measuring changes in these structures in human disease such as epilepsy, schizophrenia, Parkinson's disease, and autism spectral disorders. ..
  14. Regulation of the Endothelial Citrulline-Nitric Oxide Cycle
    Duane C Eichler; Fiscal Year: 2010
    ..enzyme that is required to maintain vascular wall function is essential to this understanding, and will potentially distinguish new therapeutic strategies for the treatment of diabetes, hypertension and related cardiovascular disease ..
  15. Vitamin D and Arterial Function in Patients with Chronic Kidney Disease
    Jessica B Kendrick; Fiscal Year: 2013
    ..Furthermore, clinical and in vitro methods will be used to identify the mechanisms by which vitamin D affects vascular endothelial function, which will enhance our understanding of the non-calcemic effects of vitamin D. ..
  16. Thiol Isomerases During Thrombus Formation
    Barbara C Furie; Fiscal Year: 2013
    ..abstract_text> ..
  17. Is symmetrical dimethylation of Arginine 2 of Histone H3 used as an epigenetic si
    Marjorie A Oettinger; Fiscal Year: 2010
    ..The experiments proposed here should define the roles of this modification and lead to insights into its possible role in both normal development and human disease. ..
  18. SIGNALING THROUGH RHO GTP/GDP EXCHANGE FACTORS
    Philip B Wedegaertner; Fiscal Year: 2013
    ..The research in this application will provide new knowledge about the functions of RhoGEFs and thus better define how the RhoGEFs can be therapeutically inhibited in disease without affecting their critical normal functions. ..
  19. Role of Lipoproteins in Chagasic heart disease
    JYOTHI FALGUNI NAGAJYOTHI; Fiscal Year: 2013
    ..This is especially important as the epidemic of obesity, diabetes and dyslipidemia in Chagas endemic regions has the potential for significant interactions with this pathogen, thus altering th normal pathogenic process. ..
  20. Control of Renal Glutaminase Expression during Acidosis
    Norman P Curthoys; Fiscal Year: 2012
    ..This knowledge will constitute the basis for biochemical and pharmacological studies that may improve the treatment of patients who present with chronic forms of metabolic acidosis. ..
  21. HORMONAL REGULATION OF MESSENGER RNA STABILITY
    Daniel R Schoenberg; Fiscal Year: 2013
    ....
  22. PTIP as an epigenetic regulator in male meiosis
    Guo Zhang Zhu; Fiscal Year: 2013
    ..Furthermore, collected information may offer insights into unexplained (or idiopathic) infertility in men and may lead to new strategies for male contraception and assisted reproduction. ..
  23. Hospital and Household Studies of Respiratory Cryptosporidiosis and Transmission
    Jeffrey K Griffiths; Fiscal Year: 2013
    ..We wish to see if it can be spread from, or to, the lungs, like influenza or tuberculosis. If it can spread in this way, then we can prevent people from getting infected by actions such as wearing masks and washing hands after coughing. ..
  24. Meiosis, SUMOylation and the ZIP3 Protein: Parallel Studies in Mouse and Yeast.
    Neil Hunter; Fiscal Year: 2012
    ..Defects in these processes are linked to human infertility, miscarriage and genetic diseases, particularly cancer. A greater understanding of their mechanism and regulation will help us better understand the etiology of these diseases. ..
  25. Multiple Molecular Mechanisms for Adiponectin Therapy in Hepatic Fibrogenesis
    Frank A Anania; Fiscal Year: 2013
    ....
  26. Molecular Mechanism of cell-cell spread of Listeria in polarized epithelial cells
    KEITH PATRICK IRETON; Fiscal Year: 2013
    ..The goal of this research is to identify bacterial and human components that mediate Listeria spreading in an enterocyte cell line. These bacterial and host proteins may be suitable as targets for drugs that limit Listeria infection. ..
  27. GABA chaperoning of epilepsy-associated GABAA receptor mutants.
    Nancy J Leidenheimer; Fiscal Year: 2012
    ..The proposed research investigates a possible mechanism for aiding the correct synthesis of these mutant receptors. Thus, these studies have relevance to the treatment of epilepsy. ..
  28. Regulation and Function of Urocortins and their Receptors
    Aditi Bhargava; Fiscal Year: 2013
    ..abstract_text> ..
  29. Release of FGF1 and Pathology of Angiogenesis
    Igor Prudovsky; Fiscal Year: 2010
    ..FGF1 is delivered to target cells through a specific stress-induced non-classical release pathway. The goal of this proposal is to understand the mechanism of FGF1 export and the role of this process in cardiovascular pathologies. ..
  30. Autophagy in epidermal melanocyte: a protective or a destructive role?
    Vijayasaradhi Setaluri; Fiscal Year: 2013
    ....
  31. Cellular Mechanisms of Lymphatic Muscle Contractility
    MICHAEL JOHN DAVIS; Fiscal Year: 2012
    ..These studies will investigate which proteins allow these vessels to contract so that specific therapeutic agents can be developed to correct lymphatic pump dysfunction and drainage of edematous tissues. ..
  32. Analysis of the Spatiotemporal Regulation of Lipolysis
    Lisa M DiPilato; Fiscal Year: 2012
    ..The information generated by this study will aid in the development of new therapeutics as well as our understanding of the mechanisms behind the spatiotemporal regulation of cellular signaling. ..
  33. Regulation of IL4 signal transduciton by poly (ADP-ribose) polymerase-1.
    Rahul Datta; Fiscal Year: 2011
    ..Given the data demonstrating that PARP-1 inhibition abates of pulmonary inflammation in the mouse model of asthma, it is important to study the role of PARP-1 in inflammation in order to develop strategies in dealing with the disease. ..
  34. IGF-II regulation of FABPs in the breast cancer survival disparity among AA women
    TELEKA CASSANDRA CALDERON; Fiscal Year: 2012
    ..Our studies will provide much needed information about the mechanisms involved in the protective effect lactation induces in the breast and will potentially offer new tools for preventive breast cancer treatment. ..
  35. Nemo-Like Kinase and the Pathogenesis of Spinal and Bulbar Muscular Atrophy
    TIFFANY WERLYNE TODD; Fiscal Year: 2013
    ....
  36. Aging and Antipsychotic Efficacy - Epigenetic Mechanisms
    Hongxin Dong; Fiscal Year: 2013
    ..By advancing our understanding of the epigenetic mechanisms of drug efficacy, it will be possible to develop new psychotropic treatment strategies that maximize benefits while minimizing side effects. ..
  37. Role of complement in alpha-synuclein based models of Parkinson disease
    HEATHER ELIZABETH ALLEN; Fiscal Year: 2013
    ..This proposal aims to provide evidence for a role for the complement system in PD pathology. This study would serve as rationale for the complement system as an innovative target for the development of PD therapies and biomarkers. ..
  38. Human Aging, Exercise &FMD: Translational Physiology
    Douglas R Seals; Fiscal Year: 2013
    .._OVIDED. University of Colorado-Boulder, Boulder, Colorado ..
  39. The role of caveolin 1 in intestinal lipid processing and transport
    JESSICA OTIS; Fiscal Year: 2013
    ....
  40. Sodium-chloride co-transporter regulation in the kidney
    Alicia A McDonough; Fiscal Year: 2013
    ....
  41. Molecular and Cellular Determinants of the Exercise Pressor Reflex in CHF
    Irving H Zucker; Fiscal Year: 2013
    ..Furthermore, w believe that these data will uncover new targets for therapy in CHF. ..
  42. Oxidative stress in regional cerebral blood flow alterations after cardiac arrest
    Mioara D Manole; Fiscal Year: 2012
    ....
  43. Lipoic Acid Therapy for Experimental Autoimmune Encephalomyelitis
    DENNIS NEIL BOURDETTE; Fiscal Year: 2013
    ..Current FDA approved therapies for MS cost up to $35,000 a year and are only partially effective. Finding new, cost effective therapies that can slow the progression of MS is of obvious relevance to the health care mission of VHA. ..
  44. Prognostic markers in metastatic melanoma
    BONNIE ELYSSA GOULDROTHBERG; Fiscal Year: 2013
    ..Thomas Lynch as Physician-in-Chief of the new cancer hospital. ..
  45. Regulation and Function of Germline RNP Granules
    JENNIFER SCHISA; Fiscal Year: 2010
    ..This research tests the hypothesis that large RNP granules play a protective role in maintaining oocyte viability and fertility and may be relevant to understanding the basis for infertility that occurs as women age. ..
  46. Soy isoflavones to augment radiotherapy of lung carcinoma
    Gilda G Hillman; Fiscal Year: 2012
    ..The results of this investigation will have high impact on the management of patients diagnosed with lung cancer. ..
  47. TYPE IV SECRETION &SIGNAL TRANSDUCTION IN EHRLICHIOSIS
    Yasuko Rikihisa; Fiscal Year: 2013
    ..abstract_text> ..
  48. INTERACTIONS BETWEEN INTERMEDIATE FILAMENTS AND NUCLEUS
    Robert D Goldman; Fiscal Year: 2013
    ..The role of lamins in the expression and elimination of abnormal chromosomes in breast cancer and prostate cancer cells will also be examined using our newly developed tools for lamin function. ..
  49. Function of the chromosomal kinase haspin in mitosis
    JONATHAN M HIGGINS; Fiscal Year: 2010
    ..A greater knowledge of the role of human haspin in this process will help us understand the defects that underlie transformation and may lead to new approaches to block the division of cancer cells. ..
  50. Protein-DNA Drug Carriers for Tumor Targeting
    Lali K Medina-Kauwe; Fiscal Year: 2012
    ..As HER2+ breast cancer does not respond well to conventional therapies, this alternative therapy could provide a significant contribution to breast cancer treatment, and could be modified to target therapy to other types of cancer. ..
  51. MECHANISM OF LACRIMAL GLAND SECRETION
    Darlene A Dartt; Fiscal Year: 2012
    ....
  52. Biology of desmosome-gap junctions in the testis
    Dolores D Mruk; Fiscal Year: 2010
    ..The study of desmosome-gap junctions in the testis will yield new and important insights into the biology of spermatogenesis. ..
  53. Using shared mechanisms of morphogenesis to study neural tube defects
    JESSICA LEE SULLIVAN-BROWN; Fiscal Year: 2012
    ..Therefore, I will study gene mutations that cause neural tube defects by using a tiny soil-dwelling worm, C. elegans, and the frog, Xenopus laevis, as simple systems to understand complex human diseases of the neural tube. ..
  54. Regulation of a Novel Trypanosome RNA Binding Protein by Arginine Methylation
    Kaylen Lott; Fiscal Year: 2013
    ..Overall, the proposed experiments will provide insight into the functions of an essential RBP in T. brucei and elucidate how arg methylation can modulate these functions. ..
  55. Role of Epithelial Chemokines in Kidney Injury-Molecule-1 Induced Renal Fibrosis
    Benjamin D Humphreys; Fiscal Year: 2010
    ..The proposed studies are therefore of great public health interest. ..
  56. In vivo mechanisms of methamphetamine-induced neuronal necrosis
    Denson G Fujikawa; Fiscal Year: 2013
    ..Our finding that METH-induced subclinical RESDs in mice are associated with widespread neuronal damage could have important translational applicability. ..
  57. Acetaldehyde and Epithelial Junctions: Role of PP2A and Occludin Phosphorylation
    MITZI LEE DUNAGAN; Fiscal Year: 2011
    ..Disruption of the barrier increases the risk of liver disease, pancreatitis, and cancer, so I am studying how acetaldehyde breaks connections between tight junction proteins and how to keep these connections intact. ..
  58. Myeloid cell interactions with T cells in atherosclerosis
    Klaus F Ley; Fiscal Year: 2013
    ..PF4 or other molecules investigated here may emerge as targets for prevention or therapy of atherosclerosis and its complications. ..
  59. Endothelial Dysfunction in Older Adult Humans with the Metabolic Syndrome
    DEMETRA CHRISTOU; Fiscal Year: 2009
    ....
  60. Modulation of Autoimmune Diabetes by N-acetyl-D-glucosamine Specific B cells
    BRIAN L DIZON; Fiscal Year: 2010
    ..Understanding the mechanisms involved in this link may provide insight on new strategies for earlier diagnosis, treatments, and prevention of T1D. ..
  61. Axial Flow Effects in Proximal Tubule
    TONG WANG WANG; Fiscal Year: 2012
    ..Possible benefits include identification of target molecules, which may be blocked or modified in order to modulate sodium reabsorption by the kidney. ..
  62. CORNEAL LIPID METABOLISM AND RESPONSE TO INFLAMMATION
    Haydee E P Bazan; Fiscal Year: 2010
    ..If our hypothesis and its corollaries are correct, then PAF antagonists can become effective therapeutic tools for maintaining the transparency and integrity of the cornea. ..
  63. GENETIC ANALYSIS OF LUPUS AUTOIMMUNITY
    Judith James; Fiscal Year: 2000
    ..This specific aim will depend upon a large genetic linkage study in human SLE currently being performed by Dr. Harley, and which already contains 30 pedigrees with a second cohort of an additional 30 pedigrees planned. ..
  64. NUCLEAR ACIDIC PROTEIN ANTIGENS IN RHEUMATIC DISEASES
    GORDON SHARP; Fiscal Year: 1990
    ..The product of autoimmune response, the immune complex, will be biochemically characterized and its distribution studied for specific autoantibody-antigen reactivities...
  65. Human Zinc Transporter Zip 13 and Cellular Zinc Homeostasis
    Joel Walker; Fiscal Year: 2007
    ..This proposal will begin to identify the genetic mechanisms controlling human Zip13 in response to low cellular zinc providing clues to how human cells regulate zinc metabolism. [unreadable] [unreadable] [unreadable]..
  66. BRAIN GLUTATHIONE S-TRANSFERASES AND NEUROTOXICITY
    Jeffrey Johnson; Fiscal Year: 2000
    ..The data generated from these studies will significantly increase our understanding of how GSTs are regulated in brain and further our knowledge of the role of these enzymes in modulating neurotoxicity. ..
  67. Development of a 3D cell culture model of the glomerular filtration barrier
    LESLIE BRUGGEMAN; Fiscal Year: 2009
    ..These studies will allow us to understand not only how individual components of the filtration barrier work, but also how they interact with regard to both normal kidney function and how it is injured in disease. ..
  68. G2/M DNA DAMAGE CHECKPOINT IN BUDDING YEAST
    Ted Weinert; Fiscal Year: 2000
    ..He will then examine interactions with other mutants. A double mutant with a mec1 constitutive and a mutant in a gene that functions downstream of MEC1 should fail to show arrest at the restrictive temperature. ..
  69. NEUROIMMUNOLOGY AND PATHOLOGY OF NEUROCYSTICERCOSIS
    JUDY TEALE; Fiscal Year: 2009
    ..In vitro studies and inhibitors of MMPs will elucidate mechanisms. The studies outlined will provide a deeper understanding of the immunopathology of NCC and should provide important insights for developing therapeutic interventions. ..
  70. AUTOANTIBODIES TO CELLULAR MATRIX ANTIGENS IN CFS
    ENG TAN; Fiscal Year: 2000
    ..Overall, these studies are aimed at rigorously defining autoantibody reactivities in CFS and may have etiologic implications. ..
  71. THE SECRETORY IGA SYSTEM OF THE EYE
    David Sullivan; Fiscal Year: 1993
    ....