blood protein electrophoresis

Summary

Summary: Electrophoresis applied to blood proteins.

Top Publications

  1. ncbi Rapid capillary electrophoretic analysis of human serum proteins: qualitative comparison with high-throughput agarose gel electrophoresis
    R Clark
    Department of Laboratory Medicine and Pathology, Mayo Clinic/Foundation, Rochester, MN 55905, USA
    J Chromatogr A 744:205-13. 1996
  2. ncbi Abnormal protein patterns in blood serum and cerebrospinal fluid detected by capillary electrophoresis
    Mariella Ivanova
    Department of Neurology, Biochemistry Laboratory, Medical University, 1 Georgy Sofijski Str, 1431 Sofia, Bulgaria
    J Biochem Biophys Methods 53:141-50. 2002
  3. ncbi A modified protein precipitation procedure for efficient removal of albumin from serum
    Yi Yun Chen
    Core Facilities for Proteomic Research, Taipei, Taiwan
    Electrophoresis 26:2117-27. 2005
  4. ncbi Comparative evaluation of serum protein analysis by capillary zone electrophoresis and agarose gel electrophoresis
    Francisco J Fernandez
    Protein Laboratory, Division of Clinical Biochemistry, University Clinic of Navarra, Avda Pio XII 36, E 31008 Pamplona, Spain
    Am Clin Lab 21:26-9. 2002
  5. ncbi Interferences in clinical capillary zone electrophoresis of serum proteins
    Xavier Bossuyt
    Laboratory Medicine Immunology, University Hospitals Leuven, Herestraat 49, B 3000 Leuven, Belgium
    Electrophoresis 25:1485-7. 2004
  6. ncbi Quantitation of serum monoclonal proteins: relationship between agarose gel electrophoresis and immunonephelometry
    David L Murray
    Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA
    Clin Chem 55:1523-9. 2009
  7. ncbi An approach to remove albumin for the proteomic analysis of low abundance biomarkers in human serum
    Nuzhat Ahmed
    Gynaecological Cancer Research Centre, Royal Women s Hospital and Department of Obstetrics and Gynaecology, University of Melbourne, Melbourne, Victoria, Australia
    Proteomics 3:1980-7. 2003
  8. ncbi Capillary electrophoresis for monitoring the effects of plasmapheresis: a feasibility study
    Anders Larsson
    Department of Medical Sciences, Clinical Chemistry, University Hospital, S 751 85 Uppsala, Sweden
    Transfus Apher Sci 33:19-23. 2005
  9. ncbi [A case of IgA2-lambda type M-protein that IgA concentration differs from the values of M-protein by serum protein electrophoresis]
    M Fukushima
    Central Clinical Laboratory, Shinshu University Hospital, Matsumoto 390-8621
    Rinsho Byori 49:690-4. 2001
  10. ncbi Linearity and detection limit in the measurement of serum M-protein with the capillary zone electrophoresis system Capillarys
    Enrique Bergón
    Department of Clinical Pathology, Hospital Universitario de Getafe, Madrid, Spain
    Clin Chem Lab Med 43:721-3. 2005

Detail Information

Publications199 found, 100 shown here

  1. ncbi Rapid capillary electrophoretic analysis of human serum proteins: qualitative comparison with high-throughput agarose gel electrophoresis
    R Clark
    Department of Laboratory Medicine and Pathology, Mayo Clinic/Foundation, Rochester, MN 55905, USA
    J Chromatogr A 744:205-13. 1996
    ....
  2. ncbi Abnormal protein patterns in blood serum and cerebrospinal fluid detected by capillary electrophoresis
    Mariella Ivanova
    Department of Neurology, Biochemistry Laboratory, Medical University, 1 Georgy Sofijski Str, 1431 Sofia, Bulgaria
    J Biochem Biophys Methods 53:141-50. 2002
    ..Furthermore, capillary electrophoresis exhibits shorter analysis time and high resolution with low baseline noise. The results were proven to be powerful in diagnosis and monitoring of dyscrasias in routine laboratory practice...
  3. ncbi A modified protein precipitation procedure for efficient removal of albumin from serum
    Yi Yun Chen
    Core Facilities for Proteomic Research, Taipei, Taiwan
    Electrophoresis 26:2117-27. 2005
    ..Albumin isolated by the modified method is in the native state. Our method may offer a rapid method for purifying serum albumin in large scale...
  4. ncbi Comparative evaluation of serum protein analysis by capillary zone electrophoresis and agarose gel electrophoresis
    Francisco J Fernandez
    Protein Laboratory, Division of Clinical Biochemistry, University Clinic of Navarra, Avda Pio XII 36, E 31008 Pamplona, Spain
    Am Clin Lab 21:26-9. 2002
  5. ncbi Interferences in clinical capillary zone electrophoresis of serum proteins
    Xavier Bossuyt
    Laboratory Medicine Immunology, University Hospitals Leuven, Herestraat 49, B 3000 Leuven, Belgium
    Electrophoresis 25:1485-7. 2004
    ..This minireview gives a survey of interfering substances, including iodinated contrast agents and antibiotics...
  6. ncbi Quantitation of serum monoclonal proteins: relationship between agarose gel electrophoresis and immunonephelometry
    David L Murray
    Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA
    Clin Chem 55:1523-9. 2009
    ..Previous comparisons of monoclonal protein quantification identified a bias between serum protein electrophoresis (SPEP) and immunonephelometry (NEPH)...
  7. ncbi An approach to remove albumin for the proteomic analysis of low abundance biomarkers in human serum
    Nuzhat Ahmed
    Gynaecological Cancer Research Centre, Royal Women s Hospital and Department of Obstetrics and Gynaecology, University of Melbourne, Melbourne, Victoria, Australia
    Proteomics 3:1980-7. 2003
    ..In conclusion, treatment of serum samples with Affi-Gel Blue or Aurum kit before 2-DE analysis can be used to remove high abundance proteins in order to increase the detection sensitivity of proteins present in low abundance...
  8. ncbi Capillary electrophoresis for monitoring the effects of plasmapheresis: a feasibility study
    Anders Larsson
    Department of Medical Sciences, Clinical Chemistry, University Hospital, S 751 85 Uppsala, Sweden
    Transfus Apher Sci 33:19-23. 2005
    ..The mean reduction in M-component size was 51%, range 33-61%. Capillary electrophoresis is a rapid and inexpensive method that could be used to monitor the effect of plasmapheresis during the actual treatment...
  9. ncbi [A case of IgA2-lambda type M-protein that IgA concentration differs from the values of M-protein by serum protein electrophoresis]
    M Fukushima
    Central Clinical Laboratory, Shinshu University Hospital, Matsumoto 390-8621
    Rinsho Byori 49:690-4. 2001
    ....
  10. ncbi Linearity and detection limit in the measurement of serum M-protein with the capillary zone electrophoresis system Capillarys
    Enrique Bergón
    Department of Clinical Pathology, Hospital Universitario de Getafe, Madrid, Spain
    Clin Chem Lab Med 43:721-3. 2005
    ..All these limitations have to be taken into account when monitoring monoclonal proteins, because the loss of linearity and the protein background may hide an increase in concentration at low levels...
  11. ncbi Reduction and alkylation of proteins in preparation of two-dimensional map analysis: why, when, and how?
    B Herbert
    Proteome Systems, North Ryde, Sydney, NSW, Australia
    Electrophoresis 22:2046-57. 2001
    ..This strongly quenches their transfer in the subsequent SDS-PAGE step...
  12. ncbi Serum protein electrophoresis in retired racing Greyhounds
    Manena Fayos
    College of Veterinary Medicine, Universidad Autonoma de Barcelona, Spain
    Vet Clin Pathol 34:397-400. 2005
    ..Compared with other breeds, Greyhounds have low serum protein concentrations, but the concentrations of different serum protein fractions have not been reported...
  13. ncbi Assessment of serum free light chain assays for plasma cell disorder screening in a Veterans Affairs population
    Jude M Abadie
    Department of Laboratory Medicine, University of Washington Medical School, Veterans Affairs Puget Sound Health Care System, Seattle, Washington 98108, USA
    Ann Clin Lab Sci 36:157-62. 2006
    ..Using the serum FLC results in conjunction with SPEP results improves the sensitivity and specificity for managing VA patients whose clinical presentation indicates the need to evaluate PCD...
  14. ncbi [A rare cause of diffuse pulmonary hemorrhage in a 45 year-old man]
    J Schreiber
    Abteilung für Pneumologie der Klinik für Innere Medizin, Städtisches Klinikum Dessau, Akademisches Lehrkrankenhaus der Martin Luther Universität Halle Wittenberg
    Internist (Berl) 47:944-51. 2006
    ..The diffuse pulmonary bleeding stopped after initiation of treatment consisting of vincristine, adriamycine and dexamethasone...
  15. ncbi Serum free light chains: an alternative to the urine Bence Jones proteins screening test for monoclonal gammopathies
    Peter G Hill
    Department of Chemical Pathology, Derbyshire Royal Infirmary, Derby Hospitals National Health Service Foundation Trust, Derby, United Kingdom
    Clin Chem 52:1743-8. 2006
    ....
  16. ncbi Serum free light chain specificity and sensitivity: a reality check
    Jerry A Katzmann
    Clin Chem 52:1638-9. 2006
  17. ncbi A rapid, economical, and reproducible method for human serum delipidation and albumin and IgG removal for proteomic analysis
    Qin Fu
    Department of Medicine, Johns Hopkins University, Baltimore, MD, USA
    Methods Mol Biol 357:365-71. 2007
    ..The reproducible depletion of lipids, IgG, and HSA permits a higher load of the remaining serum proteins, facilitating the identification of disease biomarkers...
  18. ncbi Microchip-based capillary electrophoresis of human serum proteins
    C L Colyer
    Department of Chemistry, University of Alberta, Edmonton, Canada
    J Chromatogr A 781:271-6. 1997
    ..Analysis of all five protein zones in a true human serum sample, however, has not yet been achieved on-chip due to the poor sensitivity of the TNS label for several of the serum proteins...
  19. ncbi [Detection of free light chains--a new method of diagnostics of haematological diseases]
    J Mecl
    Nemocnice Na Homolce, Praha
    Cas Lek Cesk 146:159-62. 2007
    ..To diagnose monoclonal gamopathy, one of the most frequent haematological diseases, we use immunochemical assays, which are based on the detection of paraprotein in serum and/or urine...
  20. ncbi Artificial neural network-based algorithm for the evaluation of serum protein capillary electrophoresis
    Agostino Ognibene
    Clin Chem Lab Med 42:1451-2. 2004
  21. ncbi Time course of acute-phase response induced by Tityus serrulatus venom and TsTX-I in mice
    Andréa C Pessini
    Departamento de Fisica e Quimica, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, USP, Av do Cafe, S N, 14040 903 Ribeirao Preto, SP, Brazil
    Int Immunopharmacol 3:765-74. 2003
    ..Time-course analysis of these effects shows that responses are most pronounced on the first day after poisoning. However, leucocytosis and changes in acute-phase protein concentrations can be observed up to 7 days after envenomation...
  22. ncbi Multiple myeloma diagnosed during early pregnancy: a case report
    Annamaria Maglione
    Department of Obstetrics and Gynecology, Hospital Casa Sollievo della Sofferenza, IRCCS, San Giovanni Rotondo, 71013 Foggia, Italy
    Eur J Obstet Gynecol Reprod Biol 111:214-5. 2003
    ..A cesarean section was performed at the 34th week due to the progression of the disease and a normal healthy baby was delivered...
  23. ncbi Conflicting results between electrophoresis methods of serum M-proteins
    Birgitte Wuyts
    Department of Clinical Chemistry, University Hospital Ghent, De Pintelaan 185, B 9000 Gent, Belgium
    Electrophoresis 25:1548-50. 2004
    ..5) or pentamerization of IgM M-proteins. Therefore, other causes for discrepancies should be investigated...
  24. ncbi Quality control and quality assurance aspects of the routine use of capillary electrophoresis for serum and urine proteins in clinical laboratories
    Margaret A Jenkins
    Austin Pathology, Austin Health, Heidelberg, 3084 Victoria, Australia
    Electrophoresis 25:1555-60. 2004
    ..The same applies for control material for urinary protein electrophoresis...
  25. ncbi [Serum protein immunofixation in malignant hemopathies other than multiple myeloma and Waldenstrom's macroglobulinemia]
    L Ramont
    Laboratoire Central de Biochimie, Hopital Robert Debre, Centre Hospitalier Universitaire, avenue du Général Koenig, 51092 Reims Cedex, France
    Ann Biol Clin (Paris) 60:565-70. 2002
    ....
  26. ncbi Myeloma and macroglobulinemia: what are the criteria for diagnosis?
    Marvin J Stone
    Baylor Charles A Sammons Cancer Center, Dallas 75246, TX, USA
    Clin Lymphoma 3:23-5. 2002
    ..Uniform definitions and consensus on diagnostic standards are becoming increasingly necessary if more effective and less toxic therapeutic approaches for patients with myeloma and macroglobulinemia are to be applied in an optimal manner...
  27. ncbi [Study of monoclonal gammopathy in Kagawa Prefectural Central Hospital]
    Masami Uchida
    Department of Clinical Laboratory, Kagawa Prefectural Central Hospital, Takamatsu 760 8557
    Rinsho Byori 52:574-9. 2004
    ..In addition, long-term follow-up of patients with monoclonal gammopathy and preparation of a database of patient information are useful for monitoring the outcome...
  28. ncbi [Age-related variability of serum protein antigens in sturgeons (Acipenseridae, Acipenseriformes)]
    M F Subbotkin
    Ontogenez 35:360-6. 2004
    ..The direction of ontogenetic variability of serum antigens in sturgeon fry did not depend on the habitat of adult fish in fresh or sea water...
  29. ncbi [Establishment and evaluation of the method for removing albumin and IgG in serum proteomics]
    Xue Hua Li
    Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
    Di Yi Jun Yi Da Xue Xue Bao 24:1233-5. 2004
    ..To establish and evaluate the method for removing albumin and IgG in serum proteomics...
  30. ncbi Low IgE monoclonal gammopathy level in serum highlights 20-yr survival in a case of IgE multiple myeloma
    Michael J Hayes
    Department of Pathology, The University of Michigan, Ann Arbor, MI, USA
    Eur J Haematol 78:353-7. 2007
    ..Following chemotherapy, the patient went into clinical remission, eventually dying of complications of emphysema. This case expands the recognized clinical spectrum of IgE multiple myeloma...
  31. ncbi Plasma/serum proteomics: pre-analytical issues
    Stefano Barelli
    Service Régional Vaudois de Transfusion Sanguine, Lausanne, Switzerland
    Expert Rev Proteomics 4:363-70. 2007
    ..This review aims to address some issues that can be encountered when handling plasma and serum in experimental and clinical proteomic settings...
  32. ncbi [Abundant protein' depletion in search of biomarkers]
    Alex J Rai
    Department of Clinical Laboratories, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, Box 88, New York, NY 10021, USA
    Med Sci (Paris) 23:5-8. 2007
    ..Finally, considerations for the translation of biomarkers, identified in such a manner, to a clinically useful assay, are discussed...
  33. ncbi Performance of the Sebia CAPILLARYS 2 for detection and immunotyping of serum monoclonal paraproteins
    Zhaohai Yang
    Department of Pathology, University of Alabama, Birmingham, AL 35233, USA
    Am J Clin Pathol 128:293-9. 2007
    ..In our hands, AGE and CZE had the same specificity for detection of monoclonal proteins; however, CZE/immunosubtraction seems to be less sensitive than IFE for the detection of IgM and, possibly, serum light chains...
  34. ncbi Protein electrophoresis, immunoelectrophoresis and immunofixation electrophoresis as predictors for high-risk phenotype in familial Waldenström macroglobulinemia
    Mary L McMaster
    Genetic Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD MD 20892 7236, USA
    Int J Cancer 122:1183-8. 2008
    ..Polyclonal IgM may also be a marker of genetic susceptibility in WM families. Larger studies are needed to confirm these observations...
  35. ncbi Protein profiling of human plasma samples by two-dimensional electrophoresis
    Sang Yun Cho
    Department of Biochemistry, College of Sciences, Yonsei Biomedical Proteome Research Center, Seoul, Korea
    Methods Mol Biol 428:57-75. 2008
    ..Here, we describe a typical 2DE procedure for plasma proteome under either a healthy or a diseased state (e.g., liver cancer) in which pre-fractionation and depletion are integral steps in the search for disease biomarkers...
  36. ncbi Free-flow electrophoresis system for plasma proteomic applications
    Robert Wildgruber
    BD GmbH, Martinsried, Germany
    Methods Mol Biol 424:287-300. 2008
    ..From a single FFE fraction 46 different proteins (protein family) have been identified, demonstrating FFE as a high efficient separation tool for human plasma proteome studies...
  37. ncbi Hemoglobin North Chicago (beta 36 [C2] proline----serine): a new high affinity hemoglobin
    S Rahbar
    Hemoglobin 9:559-76. 1985
    ..This residue is involved in the alpha 1 beta 2 contacts of hemoglobin molecule and its substitution to serine is possibly associated with conformational changes and alteration of hemoglobin function...
  38. ncbi Two-dimensional separation of human plasma proteins using iterative free-flow electrophoresis
    Mikkel Nissum
    BD Diagnostics, Martinsried, Germany
    Proteomics 7:4218-27. 2007
    ..g., L-selectin at 17 ng/mL and vascular endothelial-cadherin precursor at 30 ng/mL) and several tissue leakage products, thus providing a powerful orthogonal separation step in the proteomics workflow...
  39. ncbi Assessment of serum protein electrophoresis for monitoring therapy of naturally acquired equine cyathostomin infections
    J B Abbott
    Division of Companion Animal Sciences, Institute of Comparative Medicine, Faculty of Veterinary Medicine, University of Glasgow Veterinary School, Bearsden Road, Glasgow G61 1QH, UK
    Vet Parasitol 147:110-7. 2007
    ..SPE was therefore concluded to be an insensitive tool for the monitoring of cyathostomin treatment in horses in a clinical environment...
  40. ncbi Hb D-Neath or beta 121 (GH4) Glu-->Ala: a new member of the Hb D family
    S G Welch
    Department of Biochemistry, Faculty of Basic Medical Science, Queen Mary and Westfield College, London, England
    Hemoglobin 17:255-9. 1993
  41. ncbi Substitution of sugar cane bagasse in the chicken diet and immune response
    R A Hegazy
    National Center for Radiation Research and Technology, Department of Food Irridiation, Ahmed RI-Zumer St, P.O.B. 29, ET-113701 Nasr City, Cairo, Egypt
    Nahrung 45:364-7. 2001
    ..g. laying hens to overcome its suppressive effect on the gamma globulins...
  42. ncbi Renal processing of serum proteins in an albumin-deficient environment: an in vivo study of glomerulonephritis in the Nagase analbuminaemic rat
    Tanya M Osicka
    Endocrine Unit, Austin and Repatriation Medical Centre, Department of Medicine, University of Melbourne, Heidelberg, Victoria, Australia
    Nephrol Dial Transplant 19:320-8. 2004
    ....
  43. ncbi The carbonyl content of specific plasma proteins is decreased by dietary copper deficiency in rats
    Kevin A Cockell
    Nutrition Research Division, Bureau of Nutritional Sciences, Food Directorate, Health Canada, Ottawa, ON, Canada K1A 0L2
    J Nutr 132:2514-8. 2002
    ..Although dietary Cu deficiency may increase susceptibility to oxidative stress, it does not lead to accumulation of oxidized plasma proteins in this animal model...
  44. ncbi Clinical, physiological and serological observations of a human following a venomous bite by Macrovipera lebetina lebetina (Reptilia: Serpentes)
    Bayram Göçmen
    Ege University Science Faculty, Department of Zoology, Izmir, Turkey
    Turkiye Parazitol Derg 30:158-62. 2006
    ..On the other hand, the measurements carried out in blood smears and biochemical results indicated the decrease in RBC, MCV, MHV and a decrease in the diameter of red blood cells, as well as an increase in WBC...
  45. ncbi Analysis of protein/polypeptide interactions in human plasma using nondenaturing micro-2-DE followed by 3-D SDS-PAGE and MS
    Takashi Manabe
    Department of Chemistry, Graduate School of Science and Engineering, Ehime University, Matsuyama, Japan
    Electrophoresis 28:2065-79. 2007
    ..The method employed here, aiming to integrate information on the proteins separated on nondenaturing 2-DE gels with that on the interactions between polypeptides, would help the comprehensive understanding of complex protein systems...
  46. ncbi Transient paraproteinemia: an intriguing immunological anomaly
    Stephen L Strobel
    Department of Pathology, St Vincent Mercy Medical Center, Toledo, Ohio 46308 2603, USA
    Ann Clin Lab Sci 33:265-70. 2003
    ..Twelve transient paraproteins were identified in a total of 895 serum protein electrophoreses reviewed (1.3% incidence). The relationships of the transient paraproteins to specific diseases were studied...
  47. ncbi A serum protein polymorphism determinant on chromosome 9 of Mus musculus
    E M Eicher
    Mol Gen Genet 177:571-6. 1980
    ..Because the genotype of SEP-1 can be determined for individual mice without killing them, Sep-1 is a very useful gene in linkage studies and experimental biology...
  48. ncbi [Effect of tamoxifen on plasma proteins in diabetes mellitus type 1]
    Teresa Cristina P Silva
    Programa de Pós Graduação em Ciências Farmacêuticas, Centro de Ciencias da Saude, UFRN
    Acta Cir Bras 20:131-8. 2005
    ....
  49. ncbi Phylogeography of wild musk shrew (Suncus murinus) populations in Asia based on blood protein/enzyme variation
    Megumi Kurachi
    Graduate School of Bioagricultural Sciences, Nagoya University, Furocho, Chikusa, Nagoya 464 8601, Japan
    Biochem Genet 45:543-63. 2007
    ....
  50. ncbi Myelogenous leukemia in a bearded dragon (Acanthodraco vitticeps)
    M E Tocidlowski
    Houston Zoological Gardens, 1513 North MacGregor Street, Houston, Texas 77030, USA
    J Zoo Wildl Med 32:90-5. 2001
    ..The abnormal cell population of the peripheral blood did not stain with the lymphoid cytochemical stains: alpha-naphthyl butyrate esterase, CD3, and CD79a...
  51. ncbi Case-control studies of novel hemoglobin anomalies as differential diagnosis in sleep apnea syndrome
    Berndt Zur
    Department of Clinical Chemistry and Clinical Pharmacology, University of Bonn, Sigmund Freud Str 25, 53127 Bonn, Germany
    Sleep Breath 17:557-63. 2013
    ....
  52. pmc Membranoproliferative glomerulonephritis secondary to monoclonal gammopathy
    Sanjeev Sethi
    Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 1st Street SW, Rochester, MN 55905, USA
    Clin J Am Soc Nephrol 5:770-82. 2010
    ..Autoimmune diseases and chronic infections, such as hepatitis C, are commonly recognized causes of MPGN; however, monoclonal gammopathy is a less widely recognized cause of MPGN...
  53. ncbi Significance of oligoclonal bands after stem cell transplantation in multiple myeloma cases
    Ai jun Liu
    Department of Hematology, Chao Yang Hospital, Capital Medical University, Beijing, China
    Asian Pac J Cancer Prev 13:1483-6. 2012
    ..To determine the characteristics of oligoclonal bands that are frequently detected by serum protein electrophoresis (SPEP) and immunofixation electrophoresis (IFE) after stem cell transplantation...
  54. pmc A novel role for ABCA1-generated large pre-beta migrating nascent HDL in the regulation of hepatic VLDL triglyceride secretion
    Soonkyu Chung
    Department of Pathology Section on Lipid Sciences, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    J Lipid Res 51:729-42. 2010
    ....
  55. ncbi [Serum protein electrophoresis: comparison of capillary zone electrophoresis Capillarys (Sebia) and agarose gel electrophoresis Hydrasys (Sebia)]
    B Lissoir
    Laboratoire de Biologie Clinique, Service de Biochimie Medicale, Cliniques Universitaires Saint Luc, 10, Avenue Hippocrate, 1200 Bruxelles
    Ann Biol Clin (Paris) 61:557-62. 2003
    ..5%), however with a lower specificity (93.7 versus 98.9%). On the other hand, the Capillarys method displays obvious practical advantages such as full automation, ease of use and rapidity...
  56. ncbi [A "pulseless" woman with proteinuria!]
    C Garcia
    Service de Biochimie, hôpital d instruction des armées Percy, Clamart
    Ann Biol Clin (Paris) 62:441-5. 2004
    ..The association of Takayasu arteritis and renal failure is unusual. This review focuses on the biological manifestations of Takayasu arteritis and on the link between this disease and the renal failure observed in this case...
  57. ncbi Protein intensity changes in the hemoglobin and plasma electrophoretic patterns of Oreochromis niloticus in response to single and combined Zn and Cd exposure
    Ozgur Firat
    Department of Biology, Faculty of Science and Letters, Adiyaman University, 02040 Adiyaman, Turkey
    J Biochem Mol Toxicol 24:395-401. 2010
    ..Also, there was increasing level of the metals in the whole blood with increasing concentrations of metals in the exposure medium and with increasing duration of exposure...
  58. ncbi Reference intervals for biochemical analytes in serum and heparinized plasma and serum protein fractions in adult alpacas (Vicugna pacos)
    Dominic R Dawson
    Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA
    Vet Clin Pathol 40:538-48. 2011
    ..Species-specific reference intervals for laboratory tests are required for diagnosis and management of disease. Few reports of reference intervals for biochemical analytes and protein fractions are available for alpacas...
  59. ncbi Non-specific immunity-enhancing effects of tryptic casein hydrolysate versus Fermosorb for treatment/prophylaxis of newborn calf colibacillosis
    Gediminas A Biziulevicius
    Immunomodulators Research Sector, Institute of Immunology, Vilnius University, 29 Moletu plentas, LT 2021, Vilnius, Lithuania
    FEMS Immunol Med Microbiol 39:155-61. 2003
    ..The immunostimulatory activity of TCH most probably derives from a synergistic action of bioactive peptides encrypted in the preparation itself and the cell wall fragments resulting from microbial autolysis induction...
  60. ncbi Subclasses of low-density lipoprotein and very low-density lipoprotein in familial combined hyperlipidemia: relationship to multiple lipoprotein phenotype
    A M Georgieva
    Cardiovascular Research Institute Maastricht, Laboratory of Molecular Metabolism and Endocrinology, Department of Medicine, University of Maastricht, The Netherlands
    Arterioscler Thromb Vasc Biol 24:744-9. 2004
    ..The present study addresses the presence of distinct metabolic phenotypes in familial combined hyperlipidemia (FCHL) in relation to small dense low-density lipoprotein (sd LDL) and very low-density lipoprotein (VLDL) subclasses...
  61. ncbi Study of agreement between LDL size as measured by nuclear magnetic resonance and gradient gel electrophoresis
    D R Witte
    EURODIAB, Department of Epidemiology and Public Health, Royal Free and University College London Medical School, London, United Kingdom
    J Lipid Res 45:1069-76. 2004
    ..In particular, the two methods should not be assumed to be interchangeable in women and diabetic subjects. Whether NMR or GGE predicts cardiovascular disease risk better has not yet been evaluated...
  62. ncbi Combined analysis using extended renal reference range of serum free light chain ratio and serum protein electrophoresis improves the diagnostic accuracy of multiple myeloma in renal insufficiency
    Jeong Woo Park
    Department of Nephrology, Chonnam National University Medical School, Gwangju, Republic of Korea
    Clin Biochem 45:740-4. 2012
    ..To determine the diagnostic accuracy of serum free light chain κ/λ ratio (rFLC) using extended renal reference (renal range rFLC) in multiple myeloma (MM) with renal insufficiency...
  63. ncbi Multiple myeloma presenting as recurrent sinopulmonary infections
    Julia Kang
    Department of Internal Medicine, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, New Brunswick, NJ 08901, USA
    South Med J 103:260-1. 2010
    ..The correct diagnosis of multiple myeloma can be easily overlooked, especially in a patient with recurrent infections but no other signs or symptoms typical of myeloma...
  64. ncbi Molecular diagnosis of a case of Hb Phnom Penh [alpha117(GH5)Phe-I1e-alpha118(H1)Thr (alpha1)]
    Rock Y Y Leung
    Pathology Department, United Christian Hospital, Hong Kong
    Hemoglobin 30:397-9. 2006
    ..The result confirmed the predicted DNA sequence change involved in the mutation, which was delineated by another group in 1998 using amino acid analysis...
  65. ncbi Haemoglobin Lepore in a Malay family: a case report
    Josephine Pasangna
    Department of Pathology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
    Malays J Pathol 27:33-7. 2005
    ..Accurate diagnosis would provide information necessary for prenatal diagnosis, proper clinical management and genetic counseling. The clinical, haematological and laboratory features of this disorder are discussed in this paper...
  66. ncbi [Monoclonal gammopathies of undetermined significance]
    Norbert Ifrah
    Service des Maladies du Sang, CHU et UFR médecine, UPRES EA 3863, Angers
    Rev Prat 56:18-24. 2006
    ..In most MGUS, however, populations of normal and myelomatous plasma cells coexist in a stable ratio. The overall relative risk for progression is 7 times the expected rate, depending from the disease and the type of M component...
  67. ncbi [What is your diagnosis? Bisalbuminemia]]
    R Binder
    Klinik und Poliklinik fur Allgemeine Innere Medizin, Inselspital, Bern
    Praxis (Bern 1994) 95:181-2. 2006
  68. pmc Detection of kappa and lambda light chain monoclonal proteins in human serum: automated immunoassay versus immunofixation electrophoresis
    Troy D Jaskowski
    Associated Regional and University Pathologists Institute for Clinical and Experimental Pathology, University of Utah School of Medicine, 500 Chipeta Way, Salt Lake City, UT 84108, USA
    Clin Vaccine Immunol 13:277-80. 2006
    ..9 to 91.4%) than IFE, but specific in detecting serum monoclonal proteins. Moreover, the kappa/lambda ratio has little value in routine screening since the majority of sera with abnormal IFE patterns had normal kappa/lambda FLC ratios...
  69. ncbi IPG with electrodic plateaus (and other unusual procedures for 2-DE)
    Elisabetta Gianazza
    Gruppo di Studio per la Proteomica e la Struttura delle Proteine, Dipartimento di Scienze Farmacologiche, Universita degli Studi, Milano, Italia
    Electrophoresis 28:2953-6. 2007
    ..The only requirement to achieve this is to leave the all-ready-made attitude and go back to custom polymerization of the IPGs in one's laboratory...
  70. ncbi Serum biochemistry and electrophoretic patterns in the Eurasian Buzzard (Buteo buteo): reference values
    D Gelli
    Department of Veterinary Clinical Science, University of Padua, Legnaro, Padua, Italy
    J Wildl Dis 45:828-33. 2009
    ..5 g/l, creatinine 0.1 mg/dl. Mean electrophoretic values were prealbumin 1.4 g/l, albumin 14.2 g/l, alpha 1 globulin 5.9 g/l, alpha 2 globulin 4.7 g/l, beta globulin 7.5 g/l, gamma globulin 3.6 g/l, albumin/globulins ratio 0.8 g/l...
  71. ncbi Glomeruloid hemangiomas without POEMS syndrome: series of three cases
    Seth B Forman
    J Cutan Pathol 34:956-7. 2007
  72. ncbi Changes in total lipid profiles assessed by analytical capillary isotachophoresis throughout the acute phase of myocardial infarction
    Keisuke Okamura
    Int J Cardiol 108:264-6. 2006
  73. ncbi DNA vaccination with the Aleutian mink disease virus NS1 gene confers partial protection against disease
    Yurdana Castelruiz
    Laboratory of Virology and Immunology, Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Stigbøjlen 7, 1870 Frederiksberg C, Copenhagen, Denmark
    Vaccine 23:1225-31. 2005
    ..The findings let us to conclude, that the two DNA vaccinated groups of mink did show milder disease characteristics, but that the vaccine effect also in this trial could only be characterized as partial...
  74. ncbi Strategy combining separation of isotope-labeled unfolded proteins and matrix-assisted laser desorption/ionization mass spectrometry analysis enables quantification of a wide range of serum proteins
    Wei Li Liao
    Center for Advanced Research in Biotechnology, National Institute of Standards and Technology, University of Maryland Biotechnology Institute, Rockville, MD 20850, USA
    Anal Biochem 377:55-61. 2008
    ..This robust and inexpensive workflow is suitable for the quantitative profiling of protein changes in serum associated with preanalytical variables...
  75. ncbi [Differentiation of Staphylococcus aureus isolates based on phenotypical characters]
    Jacek Miedzobrodzki
    Zakład Mikrobiologii, Wydział Biochemii, Biofizyki i Biotechnologii, Uniwersytet Jagielloński w Krakowie
    Postepy Hig Med Dosw (Online) 62:322-7. 2008
    ..aureus strains with atypical biochemical characters have evolved recently. Therefore it is essential to introduce additional typing procedures using molecular biology methods without neglecting phenotypic methods...
  76. ncbi Bisalbuminemia in chronic kidney disease
    A Ahsan Ejaz
    Division of Nephrology and Hypertension, Mayo Clinic, 4500 San Pablo Road, Jacksonville, FL 32224, USA
    Clin Exp Nephrol 8:270-3. 2004
    ..The role of persistent bisalbuminemia in renal disease is uncertain...
  77. ncbi Evaluation of variation in serum globulin concentrations in dairy cattle
    Younes Chorfi
    Clinical Biochemistry Laboratory, Faculte de Medecine Veterinaire, Universite de Montreal
    Vet Clin Pathol 33:122-7. 2004
    ..Several factors may influence the concentration of serum globulins in healthy cows and thereby affect clinical interpretation; however, few studies have addressed sources of variation in globulin values...
  78. ncbi Detection by immunofixation of M proteins in hypogammaglobulinemic patients with normal serum protein electrophoresis results
    Renuka Lakshminarayanan
    Department of Pathology and Laboratory Medicine, University of California, Davis, School of Medicine, CA, USA
    Am J Clin Pathol 127:746-51. 2007
    ..We recommend reflex IFE testing in patients with hypogammaglobulinemia with a normal SPE pattern if any of the following are present: elevated alpha2- globulin/alpha1-globulin ratio, low hemoglobin level, and elevated creatinine level...
  79. ncbi [Analysis of proteomic components of sera from patients with hepatocellular carcinomas by two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time of flying mass spectrometry]
    Zheng Wang
    Department of Pathology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
    Zhonghua Bing Li Xue Za Zhi 32:333-6. 2003
    ..To analyze the proteomic components of the sera from the patients with hepatocellular carcinomas (HCC), in search of the diagnostic markers of HCC...
  80. ncbi Multiwell in-gel protein digestion and microscale sample preparation for protein identification by mass spectrometry
    Malcolm G Pluskal
    Proteome Systems, Woburn, MA 01801, USA
    Proteomics 2:145-50. 2002
    ..Data will be presented to show the application of this technology package to accelerate the throughput of protein characterization by protease fragmentation...
  81. ncbi Fibrinogen Lima. A new dysfibrinogenaemia with a high-molecular-weight alpha-chain and effective polymerization
    C L Arocha-Pinango
    Instituto Venezolano de Investigaciones Cientificas, Caracas, Venezuela
    Blood Coagul Fibrinolysis 1:561-5. 1990
    ..The molecule showed a more anodal migration than the control. The abnormality described has been classified as Fibrinogen Lima...
  82. ncbi Hb Santander [beta34(B16)Val --> Asp (GTC --> GAC)]: a new unstable variant found as a de novo mutation in a Spanish patient
    Ana Villegas
    Facultad de Medicina, Universidad Complutense de Madrid, Madrid, Spain
    Hemoglobin 27:31-5. 2003
  83. ncbi Detection of gammopathy by serum protein electrophoresis for predicting and managing therapy of lymphoproliferative disorder in 911 recipients of liver transplants
    A Lemoine
    Service de Biochimie et Biologie Moléculaire, Hopital Paul Brousse, Faculté de Médecine Paris Sud et Assistance Publique de Paris, Villejuif, France
    Blood 98:1332-8. 2001
    ..Gammopathy monitoring is an inexpensive, noninvasive, sensitive way to detect LPD and assess the efficacy of treatment. It could be used routinely in follow-up of recipients of transplants...
  84. ncbi Hb J Sicilia: beta 65 (E9) Lys-Asn, a beta homologue of Hb Zambia
    G Ricco
    FEBS Lett 39:200-4. 1974
  85. pmc A mutation in the human MPDU1 gene causes congenital disorder of glycosylation type If (CDG-If)
    C Kranz
    Klinik und Poliklinik für Kinderheilkunde, Munster, Germany
    J Clin Invest 108:1613-9. 2001
    ..This work provides a new clinical picture for another CDG that may involve synthesis of multiple types of glycoconjugates...
  86. pmc Haemoglobin Q India (alpha 64(E13) aspartic acid histidine) associated with beta-thalassemia observed in three Sindhi families
    P K Sukumaran
    J Med Genet 9:436-42. 1972
  87. ncbi Significant differences in capillary electrophoretic patterns of follicular fluids and sera from women pre-treated for in vitro fertilization
    Melinda Rezeli
    Department of Analytical Chemistry, Faculty of Sciences, University of Pecs, Ifjusag utja 6, 7624 Pecs, Hungary
    J Biochem Biophys Methods 53:151-6. 2002
    ....
  88. ncbi Characterization and structural impact of five novel PROS1 mutations in eleven protein S-deficient families
    B D Andersen
    Department of Clinical Biochemistry, Rigshospitalet, Copenhagen University Hospital, Denmark
    Thromb Haemost 86:1392-9. 2001
    ....
  89. ncbi Hemoglobin Rush (beta 101 (g3) glutamine): a new unstable hemoglobin causing mild hemolytic anemia
    J G Adams
    Blood 43:261-9. 1974
  90. pmc Abnormal haemoglobins in Zambia. A new haemoglobin Zambia alpha-60 (E9) lysine--asparagine
    G P Barclay
    Br Med J 4:595-6. 1969
    ..No evidence for beta-thalassaemia was found, but Haemoglobin H disease and other evidence for alpha-thalassaemia were found...
  91. pmc Albumin Naskapi variant in North American Indians and Eti Turks
    S G Franklin
    Proc Natl Acad Sci U S A 77:5480-2. 1980
    ....
  92. ncbi Band 3 Memphis: a widespread polymorphism with abnormal electrophoretic mobility of erythrocyte band 3 protein caused by substitution AAG----GAG (Lys----Glu) in codon 56
    P Jarolim
    Department of Biomedical Research, St Elizabeth s Hospital, Boston, MA 02135
    Blood 80:1592-8. 1992
    ....
  93. ncbi THE CHARACTERIZATION OF HEMOGLOBIN SHIMONOSEKI
    M Hanada
    Blood 24:624-35. 1964
  94. ncbi Effect of atorvastatin on total lipid profiles assessed by analytical capillary isotachophoresis
    Bo Zhang
    Cardiology 99:211-3. 2003
  95. ncbi Paraproteins and plasma cell dyscrasias
    T Karmakar
    Department of Pathology, Post Graduate Institute of Medical Education and Research, Chandigarh
    Indian J Pathol Microbiol 38:239-43. 1995
    ..Such a high incidence of PCDs with varied picture in a short time is not usually seen in other parts of this country; a fact which may be due to lack of awareness...
  96. ncbi Measurement of beta1- and beta2-globulins improves detection of M-spikes on high-resolution electrophoresis
    Srinivas Narayan
    Department of Pathology, The University of Texas Southwestern Medical Center, Dallas 75390, USA
    Clin Chem 49:676-8. 2003
  97. pmc Mass spectrometric determination of apolipoprotein molecular stoichiometry in reconstituted high density lipoprotein particles
    John B Massey
    Section of Atherosclerosis and Vascular Medicine, Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA
    J Lipid Res 50:1229-36. 2009
    ..The method has the capability to determine the molecular ratio and molecular composition of apolipoprotein molecules in complex reconstituted HDL particles...
  98. ncbi Partial characterization of serum immunoglobulins of Oreochromis mossambicus
    P B Rajavarthini
    Centre for Fish Immunology, Postgraduate and Research Department of Zoology, American College, Madurai 625002
    Indian J Exp Biol 38:549-53. 2000
    ..Further analysis of the immunoglobulin by SDS-PAGE showed that the IgM macroglobulin weighs about 900,000 Da, composed of mu-like heavy chain weighing about 90 kDa each and light chains weighing about 30 kDa each...
  99. ncbi [Homozygous sickle cell disease]
    Z Ouzzif
    Ann Biol Clin (Paris) 65:117-9. 2007
  100. ncbi Differential diagnosis of Hb EE and Hb E-beta(0)-thalassemia by protein and DNA analyses
    S C Wong
    Department of Laboratory Medicine, National University Hospital, Singapore
    Acta Haematol 103:84-9. 2000
    ..In this communication, we present the protein and DNA analyses, including data on the use of the restriction enzyme Mnl I, for the definitive diagnosis of the Hb EE genotype in the propositi of these two Malay families...
  101. ncbi Hb Southampton [beta106(G8)Leu-->Pro, CTG-->CCG] in an Argentinean boy
    Silvia Eandi Eberle
    Servicio de Hematología Oncología, Hospital de Pediatria, Prof Dr Juan P Garrahan, Buenos Aires, Argentina
    Hemoglobin 30:401-3. 2006
    ..We identified this hemoglobinopathy in a young patient with severe hemolytic anemia and hepatosplenomegaly...

Research Grants1

  1. 2007 International Peripheral Nerve Society Meeting at Snowbird, Utah
    A Smith; Fiscal Year: 2007
    ..The meeting directly impacts public health and patient care by disseminating important new ideas (both research and therapeutic) and by developing consensus guidelines. [unreadable] [unreadable] [unreadable]..