valinomycin

Summary

Summary: A cyclododecadepsipeptide ionophore antibiotic produced by Streptomyces fulvissimus and related to the enniatins. It is composed of 3 moles each of L-valine, D-alpha-hydroxyisovaleric acid, D-valine, and L-lactic acid linked alternately to form a 36-membered ring. (From Merck Index, 11th ed) Valinomycin is a potassium selective ionophore and is commonly used as a tool in biochemical studies.

Top Publications

  1. ncbi Cardiolipin biosynthesis and mitochondrial respiratory chain function are interdependent
    Vishal M Gohil
    Department of Biological Sciences, Wayne State University, Detroit, Michigan 48202, USA
    J Biol Chem 279:42612-8. 2004
  2. ncbi Selective cytotoxic activity of valinomycin against HT-29 Human colon carcinoma cells via down-regulation of GRP78
    In Ja Ryoo
    Laboratory of Antioxidant, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea
    Biol Pharm Bull 29:817-20. 2006
  3. ncbi Opening mitoKATP increases superoxide generation from complex I of the electron transport chain
    Anastasia Andrukhiv
    Dept of Biology, Portland State Univ, PO Box 751, Portland, OR 97207, USA
    Am J Physiol Heart Circ Physiol 291:H2067-74. 2006
  4. pmc Increased potassium conductance of brain mitochondria induces resistance to permeability transition by enhancing matrix volume
    Magnus J Hansson
    Mitochondrial Pathophysiology Unit, Laboratory for Experimental Brain Research, Department of Clinical Sciences, Lund University, SE 221 84 Lund, Sweden
    J Biol Chem 285:741-50. 2010
  5. ncbi Impact of Bacillus thuringiensis toxin Cry1Ab on rumen epithelial cells (REC) - a new in vitro model for safety assessment of recombinant food compounds
    Angelika Bondzio
    Department of Veterinary Biochemistry, Freie Universitat Berlin, Oertzenweg 19b, 14163 Berlin, Germany
    Food Chem Toxicol 46:1976-84. 2008
  6. pmc Mitochondrial cytochrome c release may occur by volume-dependent mechanisms not involving permeability transition
    Vladimir Gogvadze
    Division of Toxicology, Institute of Environmental Medicine, Karolinska Institutet, Box 210, SE 171 77 Stockholm, Sweden
    Biochem J 378:213-7. 2004
  7. ncbi Impedance analysis of phosphatidylcholine membranes modified with valinomycin
    Monika Naumowicz
    Institute of Chemistry, University of Bialystok, Al J Pilsudskiego 11 4, 15 443 Bialystok, Poland
    Eur Biophys J 35:239-46. 2006
  8. ncbi Effects of the ionophores valinomycin, ionomycin and gramicidin A on the element compartmentation in cultured rat hepatocytes
    S Bolkent
    University of Istanbul, Department of Biology, Istanbul, Turkey
    Toxicol In Vitro 16:159-65. 2002
  9. ncbi Effect of endogenous mutant and wild-type PINK1 on Parkin in fibroblasts from Parkinson disease patients
    Aleksandar Rakovic
    Section of Clinical and Molecular Neurogenetics, Department of Neurology, University of Lubeck, 23562 Lübeck, Germany
    Hum Mol Genet 19:3124-37. 2010
  10. ncbi Valinomycin-induced apoptosis in Chinese hamster ovary cells
    Rany Abdalah
    Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC 29425, United States
    Neurosci Lett 405:68-73. 2006

Research Grants

  1. PROTON & ELECTRON TRANSFER & ENERGY COUPLING IN SITE I
    TOMOKO NONE OHNISHI; Fiscal Year: 2010
  2. IONOPHORES, TRANSPORT AND MEMBRANE STRUCTURE
    William Duax; Fiscal Year: 1980
  3. CONTROL OF POTASSIUM TRANSPORT IN BLADDER
    PHILIP STEINMETZ; Fiscal Year: 1980
  4. APOPTOSIS INDUCED BY MICROBIAL DEPSIPETIDES
    J Cohen; Fiscal Year: 2004
  5. BINDING, TRANSPORT OF CALCIUM AND POTASSIUM IONOPHORES
    Duncan Haynes; Fiscal Year: 1980
  6. Assembly & Conductance of Fo Sector of E coli H+-ATPase
    WILLIAM BRUSILOW; Fiscal Year: 2004
  7. Modulation of NHE Activity by EPEC Infection
    Pradeep Dudeja; Fiscal Year: 2006
  8. Function and regulation of cardiac Na transporters
    Donald Hilgemann; Fiscal Year: 2008
  9. Volume Regulatory Pathways in Cystic Fibrosis Mice
    Won Kyoo Cho; Fiscal Year: 2003
  10. Antibiotics Discovery from the Great Lakes
    Yi Qiang Cheng; Fiscal Year: 2008

Detail Information

Publications224 found, 100 shown here

  1. ncbi Cardiolipin biosynthesis and mitochondrial respiratory chain function are interdependent
    Vishal M Gohil
    Department of Biological Sciences, Wayne State University, Detroit, Michigan 48202, USA
    J Biol Chem 279:42612-8. 2004
    ..in the presence of CCCP, an inhibitor of oxidative phosphorylation that reduces the pH gradient but not by valinomycin or oligomycin, both of which reduce the membrane potential and inhibit ATP synthase, respectively...
  2. ncbi Selective cytotoxic activity of valinomycin against HT-29 Human colon carcinoma cells via down-regulation of GRP78
    In Ja Ryoo
    Laboratory of Antioxidant, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea
    Biol Pharm Bull 29:817-20. 2006
    ..M126 was identified as valinomycin by various spectroscopic methods...
  3. ncbi Opening mitoKATP increases superoxide generation from complex I of the electron transport chain
    Anastasia Andrukhiv
    Dept of Biology, Portland State Univ, PO Box 751, Portland, OR 97207, USA
    Am J Physiol Heart Circ Physiol 291:H2067-74. 2006
    ..ROS production exhibited a biphasic dependence on valinomycin concentration, with peak production occurring at valinomycin concentrations that catalyze about the same K(+) ..
  4. pmc Increased potassium conductance of brain mitochondria induces resistance to permeability transition by enhancing matrix volume
    Magnus J Hansson
    Mitochondrial Pathophysiology Unit, Laboratory for Experimental Brain Research, Department of Clinical Sciences, Lund University, SE 221 84 Lund, Sweden
    J Biol Chem 285:741-50. 2010
    ..In the present study K(+) channel activity was mimicked by picomolar concentrations of valinomycin. Isolated brain mitochondria were exposed to continuous infusions of calcium...
  5. ncbi Impact of Bacillus thuringiensis toxin Cry1Ab on rumen epithelial cells (REC) - a new in vitro model for safety assessment of recombinant food compounds
    Angelika Bondzio
    Department of Veterinary Biochemistry, Freie Universitat Berlin, Oertzenweg 19b, 14163 Berlin, Germany
    Food Chem Toxicol 46:1976-84. 2008
    ..The results were compared to the effects of valinomycin, a potassium ionophore known to induce cytotoxic effects on a wide range of cells...
  6. pmc Mitochondrial cytochrome c release may occur by volume-dependent mechanisms not involving permeability transition
    Vladimir Gogvadze
    Division of Toxicology, Institute of Environmental Medicine, Karolinska Institutet, Box 210, SE 171 77 Stockholm, Sweden
    Biochem J 378:213-7. 2004
    ..In contrast with mitochondrial permeability transition-dependent release of cytochrome c, in the present study mitochondria remain intact and functionally active...
  7. ncbi Impedance analysis of phosphatidylcholine membranes modified with valinomycin
    Monika Naumowicz
    Institute of Chemistry, University of Bialystok, Al J Pilsudskiego 11 4, 15 443 Bialystok, Poland
    Eur Biophys J 35:239-46. 2006
    The effect of the ion carrier valinomycin on the electrochemical features of the phosphatidylcholine membrane was investigated by electrochemical impedance spectroscopy...
  8. ncbi Effects of the ionophores valinomycin, ionomycin and gramicidin A on the element compartmentation in cultured rat hepatocytes
    S Bolkent
    University of Istanbul, Department of Biology, Istanbul, Turkey
    Toxicol In Vitro 16:159-65. 2002
    ..electron probe X-ray microanalysis of freeze-dried cryosections after exposure of the cells to the ionophores valinomycin, ionomycin or gramicidin A...
  9. ncbi Effect of endogenous mutant and wild-type PINK1 on Parkin in fibroblasts from Parkinson disease patients
    Aleksandar Rakovic
    Section of Clinical and Molecular Neurogenetics, Department of Neurology, University of Lubeck, 23562 Lübeck, Germany
    Hum Mol Genet 19:3124-37. 2010
    ....
  10. ncbi Valinomycin-induced apoptosis in Chinese hamster ovary cells
    Rany Abdalah
    Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC 29425, United States
    Neurosci Lett 405:68-73. 2006
    ..On the other hand, the K(+) ionophore valinomycin caused substantial cell death within 12h of its application...
  11. ncbi Bacillus thuringiensis insecticidal Cry1ab toxin does not affect the membrane integrity of the mammalian intestinal epithelial cells: An in vitro study
    Nobuaki Shimada
    National Institute of Animal Health, Tsukuba, Ibaraki 305 0856, Japan
    In Vitro Cell Dev Biol Anim 42:45-9. 2006
    ....
  12. pmc Comparative analysis of antimicrobial activities of valinomycin and cereulide, the Bacillus cereus emetic toxin
    Marcel H Tempelaars
    Laboratory of Food Microbiology, Wageningen University, 6703 HD Wageningen, Netherlands
    Appl Environ Microbiol 77:2755-62. 2011
    Cereulide and valinomycin are highly similar cyclic dodecadepsipeptides with potassium ionophoric properties...
  13. pmc Differential permeabilization effects of Ca2+ and valinomycin on the inner and outer mitochondrial membranes as revealed by proteomics analysis of proteins released from mitochondria
    Akiko Yamada
    double daggerSchool of Dentistry, University of Tokushima, Kuramotocho 3, Tokushima 770 8504, Japan
    Mol Cell Proteomics 8:1265-77. 2009
    ..Our previous study showed that valinomycin also caused the release of cytochrome c from mitochondria but without inducing this PT (Shinohara, Y...
  14. ncbi Cyclosporin A does not protect the disruption of the inner mitochondrial membrane potential induced by potassium ionophores in intact K562 cells
    Luis F Marques-Santos
    Departamento de Biologia Molecular, Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraiba, Campus I, Cidade Universitaria, CEP 58059 900, Joao Pessoa, PB, Brazil
    Cell Biol Int 30:197-204. 2006
    ..carbonyl cyanide m-chlorophenylhydrazone (CCCP), potassium exchanger (nigericin), potassium ionophore (valinomycin), Na+K+-ATPase inhibitor (ouabain) and mitochondrial permeability transition pore inhibitor (cyclosporin A) ..
  15. ncbi Specific inhibition of hamster prion protein translocation by the dodecadepsipeptide valinomycin
    Jiyeon Kim
    Department of Biomedical Sciences, University of Ulsan College of Medicine, 88, Olympic ro 43 gil, Songpa Gu, Seoul 138 736, Republic of Korea
    Exp Cell Res 319:2049-57. 2013
    ....
  16. ncbi Perturbation of intracellular K(+) homeostasis with valinomycin promotes cell death by mitochondrial swelling and autophagic processes
    Barbara Klein
    Department of Cell Biology, University of Salzburg, Hellbrunnerstr 34, 5020 Salzburg, Austria
    Apoptosis 16:1101-17. 2011
    ..We investigated, which mode of cell death is induced by decreasing the intracellular K(+) concentration using valinomycin, a highly K(+)-selective ionophore...
  17. ncbi Impedance analysis of valinomycin activity in nano-BLMs
    Christian Kepplinger
    Institute for Organic and Biomolecular Chemistry, University of Gottingen, Tammannstr 2, 37077 Gottingen, Germany
    Chem Phys Lipids 160:109-13. 2009
    ..The ionophore valinomycin was successfully inserted into nano-BLMs and its transport activity monitored as a function of different ..
  18. ncbi A study of the conformations of valinomycin in solution phase
    Feng Wang
    Department of Chemistry, Vanderbilt University, Nashville, TN 37235, USA
    Biopolymers 75:85-93. 2004
    Vibrational absorption and vibrational circular dichroism (VCD) spectra of valinomycin are measured, in different solvents, in the ester and amide carbonyl stretching regions...
  19. pmc Molecular dynamics simulations of valinomycin and its potassium complex in homogeneous solvents
    T R Forester
    CCLRC Daresbury Laboratory, Daresbury, Cheshire, UK
    Biophys J 71:544-53. 1996
    ..Global structural functions, radial distribution functions, and VM ring dihedral analysis are presented, along with an analysis of the decomplexation event...
  20. pmc K+/Na+ selectivity in K channels and valinomycin: over-coordination versus cavity-size constraints
    Sameer Varma
    Computational Bioscience Department, Sandia National Laboratories, Albuquerque, NM 87185, USA
    J Mol Biol 376:13-22. 2008
    Potassium channels and valinomycin molecules share the exquisite ability to select K(+) over Na(+)...
  21. ncbi Photoelectric effects on chlorophyll fluorescence of photosystem II in vivo. Kinetics in the absence and presence of valinomycin
    Wim J Vredenberg
    Laboratory of Plant Physiology, Wageningen University and Research, Arboretumlaan 4, NL6703 BD Wageningen, The Netherlands
    Bioelectrochemistry 60:87-95. 2003
    ..curves (F(t)) in low intensity 1s light pulses have been measured in leaf discs in the presence and absence of valinomycin (VMC)...
  22. ncbi Treatment of breast tumor cells in vitro with the mitochondrial membrane potential dissipater valinomycin increases 18F-FDG incorporation
    Tim A D Smith
    John Mallard PET Centre, School of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, Scotland
    J Nucl Med 48:1308-12. 2007
    ..of mitochondrial membrane potential can be specifically induced using the K+ ion channel-opening agent valinomycin and has been used in this study to determine how the loss of mitochondrial membrane potential could influence ..
  23. ncbi Antifungal activity of valinomycin, a peptide antibiotic produced by Streptomyces sp. Strain M10 antagonistic to Botrytis cinerea
    Cheol Nam Park
    Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136 701, Korea
    J Microbiol Biotechnol 18:880-4. 2008
    ..An antifungal substance (CN1) was purified from the culture extracts of the strain, and then identified as valinomycin through extensive spectroscopic analyses...
  24. ncbi Valinomycin affects the morphology of Candida albicans
    Hiroshi Watanabe
    Department of Applied Chemistry Bioengineering, Graduate School of Engineering, Osaka City University, Sugimoto 3 3 138, Sumiyoshi ku, Osaka 558 8585, Japan
    J Antibiot (Tokyo) 58:753-8. 2005
    ..The substance was identified as valinomycin, and commercial valinomycin inhibited hyphal growth as effectively as the purified metabolite...
  25. pmc The mitochondrial toxin produced by Streptomyces griseus strains isolated from an indoor environment is valinomycin
    M A Andersson
    Department of Applied Chemistry and Microbiology, FIN 00014 University of Helsinki, Finland
    Appl Environ Microbiol 64:4767-73. 1998
    ..dodecadepsipeptide, and the fragmentation pattern obtained by tandem mass spectrometry was identical to that of valinomycin. Commercial valinomycin had effects in sperm cells that were identical to the effects of the four indoor ..
  26. ncbi Deciphering the biosynthetic codes for the potent anti-SARS-CoV cyclodepsipeptide valinomycin in Streptomyces tsusimaensis ATCC 15141
    Yi Qiang Cheng
    Department of Biological Sciences, Biotechnology Program, University of Wisconsin Milwaukee, Milwaukee, WI 53211, USA
    Chembiochem 7:471-7. 2006
    b>Valinomycin was recently reported to be the most potent agent against severe acute respiratory-syndrome coronavirus (SARS-CoV) in infected Vero E6 cells...
  27. ncbi Characterization of the cereulide NRPS alpha-hydroxy acid specifying modules: activation of alpha-keto acids and chiral reduction on the assembly line
    Nathan A Magarvey
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    J Am Chem Soc 128:10698-9. 2006
    ..Cereulide, the emetic toxin from the human pathogen Bacillus cereus, and valinomycin, from Streptomyces spp., are closely related macrocyclic K+ ionophores...
  28. ncbi Valinomycin sensitivity proves that light-induced thylakoid voltages result in millisecond phase of chlorophyll fluorescence transients
    Pavel Pospisil
    FB Physik, Freie Universitat Berlin, Arminallee 14, D 14195 Dahlem, Berlin, Germany
    Biochim Biophys Acta 1554:94-100. 2002
    ..In the presence of the ionophore valinomycin, which is known to inhibit specifically the formation of membrane voltages, the magnitude of the J-I phase is ..
  29. ncbi Mechanisms of pH regulation in the regulated secretory pathway
    M M Wu
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3200, USA
    J Biol Chem 276:33027-35. 2001
    ..However, neither steady-state pH(MSG) nor rates of passive H(+) leak were affected by Cl(-)-free solutions or valinomycin, indicating that MSG membrane potential was small and not a determinant of pH(MSG)...
  30. ncbi The higher toxicity of cereulide relative to valinomycin is due to its higher affinity for potassium at physiological plasma concentration
    Vera V Teplova
    Department of Applied Chemistry and Microbiology, Viikki Biocenter 1, POB 56, 00014 University of Helsinki, Helsinki, Finland
    Toxicol Appl Pharmacol 210:39-46. 2006
    b>Valinomycin and cereulide are bacterial toxins with closely similar chemical structure and properties but different toxic effects. Emetic poisoning is induced by cereulide but not by valinomycin. Both are specific potassium ionophores...
  31. ncbi Oxidative status of valinomycin-resistant normal, beta-thalassemia and sickle red blood cells
    Johnny Amer
    Department of Hematology, Hadassah, Hebrew University Medical Center, Ein Kerem, POB 12, 000, Jerusalem 91120, Israel
    Biochim Biophys Acta 1760:793-9. 2006
    Exposure of red blood cells (RBC) to the K+ -ionophore valinomycin (val), causes loss of KCl and water, resulting in cell dehydration, manifested by increased cell density...
  32. ncbi Entry of poliovirus into cells is blocked by valinomycin and concanamycin A
    A Irurzun
    Centro de Biologia Molecular, UAM CSIC, Universidad Autonoma de Madrid, Canto Blanco, 28049 Madrid, Spain
    Biochemistry 40:3589-600. 2001
    ..Combination of concanamycin A, a vacuolar proton-ATPase inhibitor, and valinomycin, an ionophore that promotes K(+) efflux from cells, powerfully prevented poliovirus infection...
  33. pmc Identification and characterization of a newly recognized population of high-Na+, low-K+, low-density sickle and normal red cells
    R M Bookchin
    Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 97:8045-50. 2000
    ..03%) that fail to dehydrate when permeabilized to K(+) with either valinomycin or elevated internal Ca(2+)...
  34. ncbi 13C NMR chemical shifts of the triclinic and monoclinic crystal forms of valinomycin
    Tsunenori Kameda
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112 0850, USA
    J Biomol NMR 29:281-8. 2004
    Two different crystalline polymorphs of valinomycin, the triclinic and monoclinic forms, have been studied by high resolution, solid state (13)C CP-MAS NMR spectroscopy...
  35. pmc The role of bulk protein in local models of ion-binding to proteins: comparative study of KcsA, its semisynthetic analog with a locked-in binding site, and valinomycin
    Purushottam D Dixit
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland, USA
    Biophys J 100:1542-9. 2011
    ..This local system analysis for the S(2) site of KcsA, its semisynthetic analog, and valinomycin yields the free energy change in exchanging K(+) with Na(+) in quantitative agreement with the value obtained ..
  36. ncbi Characterization of the functional coupling of bovine brain vacuolar-type H(+)-translocating ATPase. Effect of divalent cations, phospholipids, and subunit H (SFD)
    Bill P Crider
    Eugene McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center, Dallas, Texas 75390 8591, USA
    J Biol Chem 278:44281-8. 2003
    ....
  37. ncbi On-chip integration of sequential ion-sensing system based on intermittent reagent pumping and formation of two-layer flow
    H Hisamoto
    Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, Japan
    Anal Chem 73:5551-6. 2001
    ..We used KD-A3 as the lipophilic pH indicator dye and valinomycin and DD16C5 as neutral ionophores to demonstrate sequential ion sensing of potassium and sodium ions by ..
  38. ncbi Rotenone-sensitive mitochondrial potential in Phytomonas serpens: electrophoretic Ca(2+) accumulation
    Danuza Nogueira Moysés
    Departamento de Bioquimica Medica, ICB CCS, Universidade Federal do Rio de Janeiro UFRJ, Ilha do Fundao, 21941 590 Rio de Janeiro, Brazil
    Biochim Biophys Acta 1656:96-103. 2004
    ..13+/-0.23 nmol Ca(2+) mg x protein(-1) min(-1). It is completely abolished by the H(+) ionophore FCCP and by valinomycin and nigericin...
  39. ncbi Nystatin and valinomycin induce tubuloglomerular feedback
    Y Ren
    Hypertension and Vascular Research Division, Henry Ford Hospital, Detroit, Michigan 48202, USA
    Am J Physiol Renal Physiol 281:F1102-8. 2001
    ..0 +/- 1.5 to 15.6 +/- 1.6 mm; P = 0.003). When valinomycin, a K(+)-selective ionophore, was added to the macula densa lumen together with furosemide in the presence of ..
  40. ncbi Delayed shrinkage triggered by the Na+-K+ pump in terbutaline-stimulated rat alveolar type II cells
    Keita Hosoi
    Department of Physiology, Osaka Medical College, Takatsuki, 569 8686, Japan
    Exp Physiol 89:373-85. 2004
    ..This [K+]i increase, in turn, hyperpolarizes membrane potential. Valinomycin (a K+ ionophore), which induces hyperpolarization, decreased the volume of AT-II cells and enhanced the third ..
  41. ncbi Mitochondrial sequestration and Ca(2+)-dependent release of cytosolic Zn(2+) loads in cortical neurons
    Stefano L Sensi
    Department of Neurology, University G D Annunzio, Chieti, 66013, Italy
    Neurobiol Dis 10:100-8. 2002
    ..of the mitochondrial protonophore, FCCP, the mitochondrial uncoupler, dinitrophenol with the K(+) ionophore, valinomycin, or the inducer of mitochondrial permeability transition (mPT), phenylarsine oxide (PAO), to substitute for ..
  42. ncbi Phenylphosphonate transport by Helicobacter pylori
    Justin L Ford
    School of Medical Sciences, The University of New South Wales, Sydney, NSW 2052, Australia
    Helicobacter 12:609-15. 2007
    ..However, H. pylori does not have any genes homologous to those of the known pathways for phosphonate degradation in bacteria, indicating that it must have novel pathways for the transport and metabolism of phosphonates...
  43. ncbi Interference of MI-D, a new mesoionic compound, on artificial and native membranes
    Silvia M S C Cadena
    Departamento de Bioquimica, Universidade Federal do Parana, Curitiba, Brasil
    Cell Biochem Funct 20:31-7. 2002
    ..chloride), a new mesoionic compound, decreased the rate of swelling induced by valinomycin-K+, as well as induced swelling in the presence of nigericin-K+...
  44. ncbi The Na+-translocating ATPase in the plasma membrane of the marine microalga Tetraselmis viridis catalyzes Na+/H+ exchange
    Yurii V Balnokin
    Timiryazev Institute of Plant Physiology RAS, Botanicheskaya 35, 127276 Moscow, Russia
    Planta 219:332-7. 2004
    ..The (Na(+)+ATP)-dependent lumen alkalization was not significantly affected by valinomycin, addition of which in the presence of K+ abolished deltapsi at the vesicle membrane...
  45. ncbi Evidence for a gamma-hydroxybutyrate (GHB) uptake by rat brain synaptic vesicles
    Claude Muller
    Institut de Chimie Biologique and INSERM U 338, Strasbourg, France
    J Neurochem 80:899-904. 2002
    ....
  46. ncbi Analysis of phenomena involved in the apical growth of Phycomyces blakesleeanus
    Jose Ruiz-Herrera
    Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigacion y de Estudios Avanzados del IPN, Irapuato, Gto, Mexico
    Arch Microbiol 180:427-33. 2003
    ..exchanger A23187 and the K(+)/H(+) exchanger nigericin, the electrogenic membrane-potential depleters valinomycin and CCCP, and the calcium channel blockers ruthenium red, nifedipine, and nitrendipine on the apical growth of ..
  47. pmc Age decline in the activity of the Ca2+-sensitive K+ channel of human red blood cells
    Teresa Tiffert
    Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge, UK
    J Gen Physiol 129:429-36. 2007
    ..When K(+) permeabilization was induced with valinomycin, the oldest and densest cells, which started nearest to the set density boundary, crossed it first, reflecting ..
  48. ncbi K+-selective nanospheres: maximising response range and minimising response time
    Maria Jose Ruedas-Rama
    Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge, UK
    Analyst 131:1282-91. 2006
    ..ETH 5294) and lipophilic salt (KTClPB) for H(+)-sensors, or ETH 5294, a K(+)-selective ionophore (valinomycin) and anionic sites for K(+)-sensors were compared, and the effect of varying the normalised concentrations for ..
  49. ncbi Relationship between cell morphology and intracellular potassium concentration in Candida albicans
    Hiroshi Watanabe
    Department of Applied Chemistry and Bioengineering, Graduate School of Engineering, Osaka City University, Sugimoto 3 3 138, Sumiyoshi ku, Osaka 558 8585, Japan
    J Antibiot (Tokyo) 59:281-7. 2006
    Previously we reported that valinomycin inhibited hyphal growth and induced growth as a chain of yeast cells under hyphal growth induction conditions in Candida albicans...
  50. pmc Studies on the mode of action of reutericyclin
    Michael G Ganzle
    Lehrstuhl für Technische Mikrobiologie, TU Munchen, 85350 Freising, Germany
    Appl Environ Microbiol 69:1305-7. 2003
    ..A comparison of reutericyclin activity with those of nisin, nigericin, and valinomycin demonstrated that reutericyclin does not form pores but selectively dissipates the transmembrane proton ..
  51. ncbi Volume regulation is defective in renal proximal tubule cells isolated from KCNE1 knockout mice
    I D Millar
    Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield, S10 2TN, UK
    Exp Physiol 89:173-80. 2004
    ..RVD in the knockout cells was rescued by valinomycin, a K+ ionophore...
  52. ncbi Stimulation of voltage-dependent calcium channels during capacitation and by progesterone in human sperm
    Marco T Gonzalez-Martinez
    Departamento de Farmacologia, Facultad de Medicina, Universidad Nacional Autonoma de Mexico, Ciudad Universitaria, Apartado Postal 70 297, CP 04510, Mexico, DF, Mexico
    Arch Biochem Biophys 408:205-10. 2002
    ..The observed effects of capacitation and progesterone on VDCC may be physiologically significant for sperm-egg interaction...
  53. ncbi Mitochondrial Ca2+-induced K+ influx increases respiration and enhances ROS production while maintaining membrane potential
    Andre Heinen
    Anesthesiology Research Laboratories, Department of Anesthesiology, Medical College of Wisconsin, M4280, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Am J Physiol Cell Physiol 292:C148-56. 2007
    ..To test this hypothesis, we measured effects of NS-1619, a putative mtK(Ca) channel opener, and valinomycin, a K(+) ionophore, on mitochondrial respiration, DeltaPsi(m), and ROS generation in guinea pig heart ..
  54. ncbi Impaired regulatory volume decrease in freshly isolated cholangiocytes from cystic fibrosis mice: implications for cystic fibrosis transmembrane conductance regulator effect on potassium conductance
    Won Kyoo Cho
    Department of Medicine, Division of Gastroenterology Hepatology, Indiana University School of Medicine and The Richard L Roudebush Veterans Affairs Medical Center, Indianapolis, Indiana 46202, USA
    J Biol Chem 279:14610-8. 2004
    ..01 (n = 53) in 10 min but without significant recovery. Coadministration of the K(+)-selective ionophore valinomycin restored RVD in CF mouse BDCCs, suggesting that the impaired RVD was likely from a defect in K(+) conductance...
  55. pmc Vesicle transmembrane potential is required for translocation to the cytosol of externally added FGF-1
    Jedrzej Małecki
    Institute for Cancer Research, The Norwegian Radium Hospital, 0310 Montebello, Oslo, Norway
    EMBO J 21:4480-90. 2002
    ..A combination of ionophores expected to dissipate the vesicular membrane potential (valinomycin plus monensin) also fully inhibited the translocation...
  56. ncbi BCL-2 does not control programmed cell death in the IPLB-LdFB cell line from the insect Lymantria dispar
    Enzo Ottaviani
    Department of Animal Biology, University of Modena and Reggio Emilia, Italy
    Cell Biol Int 26:563-6. 2002
    ..Similar behaviour was observed using the K+ ionophore valinomycin. From these findings, we hypothesize that the L...
  57. ncbi Roles of the Na,K-ATPase alpha4 isoform and the Na+/H+ exchanger in sperm motility
    Alison L Woo
    Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267, USA
    Mol Reprod Dev 62:348-56. 2002
    ..These data, along with the observation that the K+ ionophore valinomycin has no effect on the motility of ouabain-inhibited sperm, suggest that ouabain may change intracellular H+ ..
  58. ncbi The fetal and neonatal brain protein neuronatin protects PC12 cells against certain types of toxic insult
    Shuang Zheng
    Department of Biological Chemistry, School of Medicine, University of California, Los Angeles, 650 Charles Young Drive, Los Angeles, CA 90095, USA
    Brain Res Dev Brain Res 136:101-10. 2002
    ..neuronatin and it exhibits markedly increased sensitivity to the toxic effects of nigericin, rotenone and valinomycin. Pretreatment of the 1...
  59. ncbi Crosstalk between nitric oxide and zinc pathways to neuronal cell death involving mitochondrial dysfunction and p38-activated K+ channels
    Ella Bossy-Wetzel
    Center for Neuroscience and Aging, The Burnham Institute, La Jolla, CA 92037, USA
    Neuron 41:351-65. 2004
    ..Thus, these data establish a new form of crosstalk between NO and Zn2+ apoptotic signal transduction pathways that may contribute to neurodegeneration...
  60. ncbi Lack of voltage-dependent calcium channel opening during the calcium influx induced by progesterone in human sperm. Effect of calcium channel deactivation and inactivation
    Alberto Martin Guzman-Grenfell
    Departamento de Farmacologia, Facultad de Medicina, Universidad Nacional Autonoma de Mexico, Ciudad Universitaria, Mexico, D F, Mexico
    J Androl 25:117-22. 2004
    ..Furthermore, the progesterone and voltage-dependent calcium influxes were additive. These findings indicate that progesterone does not produce VDCC gating in human sperm...
  61. ncbi Ion transport coupled to terminal oxidase functioning in the extremely alkaliphilic halotolerant bacterium Thioalkalivibrio
    Yu V Grischuk
    Belozersky Institute of Physico Chemical Biology, Lomonosov Moscow State University, Moscow, 119992, Russia
    Biochemistry (Mosc) 68:385-90. 2003
    ..that: 1) unlike other bacteria known, this bacterium did not acidify the medium in the presence of K(+) and valinomycin; 2) in the presence of an uncoupler, CCCP, but in the absence of valinomycin, reversible alkalinization of the ..
  62. ncbi [Localization of ATPase activity, respiration and ultrastructure of wheat root cells with modulated ion conductivity of plasma membrane]
    F A Chernysheva
    Institute of Biochemistry and Biophysics, Research Center RAS, Kazan
    Tsitologiia 46:221-8. 2004
    ..A 2 h treatment of excised root with valinomycin (20 microM), N,N-dicyclohexylcarbodiimid (100 microM), gramicidin S (20 microM) and chlorpromazine (100 microM) ..
  63. ncbi Effects of saturated long-chain N-acylethanolamines on voltage-dependent Ca2+ fluxes in rabbit T-tubule membranes
    Murat Oz
    National Institute on Drug Abuse, Cellular Neurobiology Section, 5500 Nathan Shock Drive, Baltimore, MD 21224, USA
    Arch Biochem Biophys 434:344-51. 2005
    ..membrane potentials were generated by establishing potassium gradients across the vesicle using the ionophore valinomycin. Arachidonoylethanolamide and docosaenoylethanolamide but not palmitoylethanolamide and stearoylethanolamide (..
  64. ncbi Reversible inhibition of the platelet procoagulant response through manipulation of the Gardos channel
    Jef L Wolfs
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, PO Box 616, 6200 MD Maastricht, The Netherlands
    Blood 108:2223-8. 2006
    ..Inhibition by Gardos channel blockers was reversed by valinomycin, a selective K+ ionophore...
  65. ncbi [The influence of plasma membrane ion permeability modulators on superoxide formation and bioelectrical characteristics of wheat root cells]
    V Ia Alekseeva
    Tsitologiia 47:578-83. 2005
    ..It was shown that a 2 h treatment of excised roots with valinomycin (Val, 20 microM), N, N'-dicyclohexylcarbodimide (DCCD, 100 microM), gramicidin S (Gr, 20 microM), ..
  66. pmc TRPV1 regulators mediate gentamicin penetration of cultured kidney cells
    Sigrid E Myrdal
    Oregon Hearing Research Center, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    Hear Res 204:170-82. 2005
    ..Membrane depolarization, induced by high extracellular K+ or valinomycin, also reduced GTTR uptake, suggesting electrophoretic permeation through ion channels...
  67. pmc A combined experimental and quantum chemical study on the putative protonophoric activity of thiocyanate
    Peter Schonfeld
    Institut fur Biochemie, Otto von Guericke Universitat Magdeburg, D 39120 Magdeburg, Germany
    Biophys J 89:1504-15. 2005
    ..Due to the easier removal of the hydration shell of SCN(-) relative to Cl(-), SCN(-) is favored in going across the membrane, giving rise to the protonophoric activity...
  68. ncbi Fluorescence measurements of free [Mg2+] by use of mag-fura 2 in Salmonella enterica
    Elisabeth M Froschauer
    Max F Perutz Laboratories, Department of Microbiology and Genetics, University of Vienna, Campus Vienna Biocenter, Dr Bohrgasse 9, A 1030 Vienna, Austria
    FEMS Microbiol Lett 237:49-55. 2004
    ..which increased the membrane potential DeltaPsi at the expense of DeltapH, and drastically reduced by valinomycin, which decreased the membrane potential DeltaPsi...
  69. ncbi The effect of the presence of valinomycin on the interfacial tension of lecithin membrane
    Aneta D Petelska
    Institute of Chemistry, University of Bialystok, Al J Pilsudskiego 11 4, 15 443 Bialystok, Poland
    Colloids Surf B Biointerfaces 44:158-62. 2005
    The effect of the presence of valinomycin in lecithin membrane on its interfacial tension has been studied...
  70. ncbi Twofold effect of valinomycin on isolated spinach chloroplasts: uncoupling and inhibition of electron transport
    A Telfer
    Botany Department, Imperial Collage, Prince Consort Road, London, Great Britain
    Biochim Biophys Acta 333:343-52. 1974
    1) Treatment of isolated chloroplasts with valinomycin above 10(-7) M inhibits both photophosphorylation and ms-delayed light emission, with maximum inhibition occurring at 10(-5) M...
  71. ncbi Influence of state-2 transition on the proton motive force across the thylakoid membrane in spinach chloroplasts
    Ji Hu Su
    Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
    Photosynth Res 85:235-45. 2005
    ..The time course was not affected significantly by valinomycin and DBMIB...
  72. ncbi Phenolsulfonphthalein transport by potential-sensitive urate transport system
    Shirou Itagaki
    Department of Clinical Pharmaceutics and Therapeutics, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12 jo, Nishi 6 chome, Kita ku, Sapporo 060 0812, Japan
    Eur J Pharmacol 518:83-9. 2005
    ..These results suggest that phenolsulfonphthalein has high affinity for the potential-sensitive urate transport system but has low affinity for an anion exchanger...
  73. ncbi Energy coupling to nitrate uptake into the denitrifying cells of Paracoccus denitrificans
    Igor Kucera
    Department of Biochemistry, Faculty of Science, Masaryk University, Kotlarska 2, CZ 61137 Brno, Czech Republic
    Biochim Biophys Acta 1709:113-8. 2005
    ..that dissipate the individual components of the proton motive force (short-chain fatty acids, nigericin, and valinomycin) upon the methyl viologen-coupled nitrate reductase activity in intact cells...
  74. ncbi Effects of inhibitors and NaCl on the oxidation of reduced inorganic sulfur compounds by a marine acidophilic, sulfur-oxidizing bacterium, Acidithiobacillus thiooxidans strain SH
    Kazuo Kamimura
    Department of Biological Function, Faculty of Agriculture, Okayama University, 1 1 1 Tsushima naka, Okayama 700 8530, Japan
    Extremophiles 9:45-51. 2005
    ..Thiosulfate-oxidizing activity was not inhibited by these uncouplers. Valinomycin did not inhibit the oxidation of sulfur compounds...
  75. ncbi Proton motive force-dependent Hoechst 33342 transport by the ABC transporter LmrA of Lactococcus lactis
    H Bart van den Berg van Saparoea
    Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, NL 9751 NN Haren, The Netherlands
    Biochemistry 44:16931-8. 2005
    ..Our data demonstrate that the LmrA-mediated Hoechst 33342 transport in membrane vesicles is influenced by the transmembrane pH gradient due to a pH-dependent partitioning of Hoechst 33342 into the membrane...
  76. ncbi Determination of the rate of K(+) movement through potassium channels in isolated rat heart and liver mitochondria
    Piotr Bednarczyk
    Department of Biophysics, Warsaw University Life of Science SGGW, Warsaw, Poland
    Biochim Biophys Acta 1777:540-8. 2008
    ..Here we used increasing concentrations of K(+)-ionophore (valinomycin) to stimulate respiration by rat liver and heart mitochondria in the presence of the K(+)/H(+) exchanger ..
  77. ncbi Differentiated Paju cells have increased resistance to toxic effects of potassium ionophores
    Vera Teplova
    Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, RU 142290 Pushchino, Moscow Region, Russia
    Acta Biochim Pol 51:539-44. 2004
    ..Addition of the highly toxic potassium ionophores cereulide (4.5 and 9.0 ng/ml) or valinomycin (20 ng/ml), to cultures of undifferentiated Paju cells caused collapse of the mitochondrial membrane potential -..
  78. ncbi Carvedilol in heart mitochondria: protonophore or opener of the mitochondrial K(ATP) channels?
    P J Oliveira
    Centro de Neurociências de Coimbra Dep Zoologia, Faculdade de Ciencias e Tecnologia, Universidade de Coimbra, Portugal
    Life Sci 69:123-32. 2001
    ..By contrast, a K(ATP)-channel inhibitor, 5-hydroxydecanoic acid, did not affect carvedilol-induced depolarizations. Hence, our results suggest that carvedilol depresses mitochondrial delta psi by a weak protonophoretic mechanism...
  79. ncbi A collection of yeast mutants selectively resistant to ionophores acting on mitochondrial inner membrane
    Silvia Petrezselyova
    Department of Genetics, Comenius University, Faculty of Natural Sciences, Mlynska dolina, 842 15 Bratislava, Slovak Republic
    Mitochondrion 8:117-29. 2008
    b>Valinomycin and nigericin are potassium ionophores acting selectively on the mitochondrial inner membrane of Saccharomyces cerevisiae [Kovac, L., Bohmerova, E., Butko, P., 1982a. Ionophores and intact cells. I...
  80. ncbi A high-throughput assay for mitochondrial membrane potential in permeabilized yeast cells
    E Farrelly
    Tularik Inc, Two Corporate Drive, South San Francisco, California 94080, USA
    Anal Biochem 293:269-76. 2001
    ..The fluorescence intensity responded vigorously to specific modulators in UCP1-expressing cells. This method has been adapted as a high-throughput assay to screen for modulators of mitochondrial membrane potential...
  81. ncbi Intracellular calibration of the fluorescent Mg2+ indicator furaptra in rat ventricular myocytes
    M Watanabe
    Department of Physiology, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan
    Pflugers Arch 442:35-40. 2001
    ..After the application of ionophores (ionomycin, monensin, nigericin and valinomycin), an immediate change in furaptra R was noted, followed by a slow change in R that reached a steady level in 2-..
  82. ncbi Voltammetric study on ion transport across a bilayer lipid membrane in the presence of a hydrophobic ion or an ionophore
    Osamu Shirai
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake cho, Sakyo ku, Kyoto, 606 8502, Japan
    Anal Bioanal Chem 386:494-505. 2006
    ..from one aqueous phase (W1) to another (W2) across a bilayer lipid membrane (BLM) containing a hydrophobic ion, valinomycin (Val) or gramicidin A (GA)...
  83. ncbi Activation of K+ channels induces apoptosis in vascular smooth muscle cells
    S Krick
    Division of Pulmonary and Critical Care Medicine, UCSD Medical Center, 200 W Arbor Dr, San Diego, CA 92103 8382, USA
    Am J Physiol Cell Physiol 280:C970-9. 2001
    ..K+ concentration from 5 to 40 mM also attenuated PASMC apoptosis induced by FCCP and the K+ ionophore valinomycin. These results suggest that FCCP-mediated apoptosis in PASMC is partially due to an increase of maxi-K channel ..
  84. ncbi Negative control of store-operated Ca2+ influx by B cell receptor cross-linking
    A Hashimoto
    Department of Pharmacology, Graduate School of Medicine, University of Tokyo, Japan
    J Immunol 166:1003-8. 2001
    ..When membrane potential was held constant by the transmembrane K(+) concentration gradient in the presence of valinomycin, the BCR-mediated inhibition of SOC was still observed...
  85. ncbi The role of ABC transporters from Aspergillus nidulans in protection against cytotoxic agents and in antibiotic production
    A C Andrade
    Laboratory of Phytopathology, Wageningen University, The Netherlands
    Mol Gen Genet 263:966-77. 2000
    ..hypersensitive phenotype to compounds such as cycloheximide, the cyclosporin derivative PSC 833, nigericin and valinomycin, indicating that AtrDp is involved in protection against cytotoxic compounds...
  86. ncbi Rubidium-86 uptake and energy metabolism in suspended human erythrocytes monitored by microdialysis
    I Ljungberg
    Department of Public Health and Clinical Medicine, Umea University, Umea, Sweden
    J Int Med Res 32:384-91. 2004
    ..Erythrocytes were also treated with the K+ ionophore valinomycin or the Na+/K+-ATPase inhibitor ouabain...
  87. pmc Reduced DIDS-sensitive chloride conductance in Ae1-/- mouse erythrocytes
    Seth L Alper
    Molecular and Vascular Medicine Unit, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    Blood Cells Mol Dis 41:22-34. 2008
    ..Thus, loss of one or more distinct, DIDS-sensitive anion channel polypeptide(s) from the Ae1(-/-) red cell membrane cannot be ruled out as an explanation for the reduced DIDS-sensitive anion conductance...
  88. ncbi Potassium ion transport by valinomycin across a Hg-supported lipid bilayer
    Lucia Becucci
    Department of Chemistry, Florence University, 50019 Sesto Fiorentino, Florence, Italy
    J Am Chem Soc 127:13316-23. 2005
    ..and electrochemical impedance spectroscopy, both in the absence and in the presence of the ionophore valinomycin. Impedance spectra, recorded from 1 x 10(-2) to 1 x 10(5) Hz over a potential range of 0...
  89. ncbi Characterization of the redox components of transplasma membrane electron transport system from Leishmania donovani promastigotes
    Tanmoy Bera
    Division of Medicinal Biochemistry, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India
    Biochim Biophys Acta 1725:314-26. 2005
    ..cells on glucose substrate shut down almost completely upon addition of an uncoupler FCCP and K+-ionophore valinomycin. The most pronounced effects on O2 uptake were obtained by treatment with antimycin A, 2-heptadecyl-4-..
  90. ncbi Effect of natural amphipathic peptides on viability, membrane potential, cell shape and motility of mollicutes
    L Beven
    Groupe Membranes et Osmorégulation, UPRES A CNRS Q6026, Universite de Rennes 1, Rennes, France
    Res Microbiol 148:163-75. 1997
    ..than bee-venom mellitin and most of the peptides produced by bacteria (globomycin, gramicidin S, surfactin and valinomycin) or fungi (alamethicin)...
  91. ncbi Potential antivirals and antiviral strategies against SARS coronavirus infections
    Erik De Clercq
    Rega Institute for Medical Research, KU Leuven, Minderbroedersstraat 10B 3000 Leuven, Belgium
    Expert Rev Anti Infect Ther 4:291-302. 2006
    ..of action but selectivity indexes up to 100, have been reported to exhibit in vitro activity against SARS-CoV: valinomycin, glycopeptide antibiotics, plant lectins, hesperetin, glycyrrhizin, aurintricarboxylic acid, chloroquine, ..
  92. ncbi The mechanism of glucose 6-phosphate transport by Escherichia coli
    L A Sonna
    Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
    J Biol Chem 263:6625-30. 1988
    ..took up Glc-6-P to levels 100-fold above medium concentration in a reaction unaffected by the ionophores valinomycin, valinomycin plus nigericin, and carbonyl cyanide p-trifluoromethoxyphenylhydrazone...
  93. ncbi Glutamine transport in rat brain synaptic and non-synaptic mitochondria
    B Roberg
    Neurochemical Laboratory, University of Oslo, Norway
    Neurochem Res 24:383-90. 1999
    ..This uptake is inhibited by valinomycin in the presence of K+ and is thus also activated by the membrane potential...
  94. pmc Cereulide synthetase gene cluster from emetic Bacillus cereus: structure and location on a mega virulence plasmid related to Bacillus anthracis toxin plasmid pXO1
    Monika Ehling-Schulz
    Lehrstuhl für Mikrobielle Ökologie, Department für biowissenschaftliche Grundlagen, WZW, Technische Universitat Munchen, Freising, Germany
    BMC Microbiol 6:20. 2006
    Cereulide, a depsipeptide structurally related to valinomycin, is responsible for the emetic type of gastrointestinal disease caused by Bacillus cereus...
  95. ncbi Renal handling of taurine, L-alanine, L-glutamate and D-glucose in Opsanus tau: studies on isolated brush border membrane vesicles
    N A Wolff
    Division of Biology and Medicine, Brown University, Rhode Island 02906
    J Comp Physiol B 157:573-81. 1987
    ..At high extravesicular K+, the addition of valinomycin reduced taurine uptake...
  96. pmc A bacitracin-resistant Bacillus subtilis gene encodes a homologue of the membrane-spanning subunit of the Bacillus licheniformis ABC transporter
    Reiko Ohki
    Department of Molecular Biolog, School of Health Sciences, Kyorin University, 476 Miyashita, Hachioji, Tokyo 192 0005, Japan
    J Bacteriol 185:51-9. 2003
    ..Resistance to other drugs such as surfactin, iturin A, vancomycin, tunicamycin, gramicidin D, valinomycin and several cationic dyes were not changed in the ywoA disruptant...
  97. ncbi 4,6-Dinitro-o-cresol uncouples oxidative phosphorylation and induces membrane permeability transition in rat liver mitochondria
    R F Castilho
    Departamento de Patologia Clínica NMCE, Faculdade de Ciencias Medicas, Universidade, Estadual de Campinas, SP, Brazil
    Int J Biochem Cell Biol 29:1005-11. 1997
    ..was evidenced by the induction of mitochondrial swelling in hyposmotic K(+)-acetate medium, in the presence of valinomycin. At higher concentrations (> 50 microM), DNOC also induces an inhibition of succinate-supported respiration, ..
  98. ncbi ATP depletion does not account for apoptosis induced by inhibition of mitochondrial electron transport chain in human dopaminergic cells
    Masahiko Watabe
    Department of Pharmacology, Teikyo University School of Medicine, 2 11 1 Kaga, Itabashi Ward, Tokyo 173 8605, Japan
    Neuropharmacology 52:536-41. 2007
    ..phosphorylation inhibitors (OPIs), including a specific F(0)F(1)ATP synthase inhibitor oligomycin, an ionophore valinomycin and an uncoupler 2,4-dinitrophenol, as tools to deplete only ATP without influencing the ETC...
  99. ncbi Effect of sydnone SYD-1, a mesoionic compound, on energy-linked functions of rat liver mitochondria
    Gerusa Clazer Halila
    Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Parana, Curitiba, Parana, Brazil
    Chem Biol Interact 169:160-70. 2007
    ..membrane, as demonstrated by assays of mitochondrial swelling in the presence of sodium acetate and valinomycin +K(+). SYD-1 (1...
  100. ncbi Reconstitution into liposomes of the B degrees -like glutamine-neutral amino acid transporter from renal cell plasma membrane
    Francesca Oppedisano
    Department of Cell Biology, University of Calabria, Via P Bucci 4c, 87036 Arcavacata di Rende, Italy
    Biochim Biophys Acta 1778:2258-65. 2008
    ..The transporter showed low if there is any tolerance towards the substitution of Na+ or K+ for other cations. Valinomycin strongly stimulated the transport indicating that it is electrogenic. Intraliposomal glutamine had no effect...
  101. ncbi Control of proteoliposomal cytochrome c oxidase: the partial reactions
    P Nicholls
    Department of Biological Sciences, Brock University, St Catharines, Ont, Canada
    Biochem Cell Biol 68:1135-41. 1990
    ..The maximal aerobic steady-state reduction level of cytochrome a is highest in the presence of valinomycin and lowest in the presence of nigericin...

Research Grants19

  1. PROTON & ELECTRON TRANSFER & ENERGY COUPLING IN SITE I
    TOMOKO NONE OHNISHI; Fiscal Year: 2010
    ..demonstrating that the signal is enhanced with an inside-positive membrane potential which was induced by the K-valinomycin method...
  2. IONOPHORES, TRANSPORT AND MEMBRANE STRUCTURE
    William Duax; Fiscal Year: 1980
    ..graphics and force field energy calculations (1) solid state data concerning complexed and uncomplexed forms of valinomycin, monensin, and other ionophores will be used to model the dynamics of ion capture and release processes, (2) ..
  3. CONTROL OF POTASSIUM TRANSPORT IN BLADDER
    PHILIP STEINMETZ; Fiscal Year: 1980
    ..the luminal cell membrane is directly accessible to translocator molecules for potassium, either ionophores (valinomycin) or channel forming agents (nystatin, gramicidin), the effects of such translocators on transepithelial as well ..
  4. APOPTOSIS INDUCED BY MICROBIAL DEPSIPETIDES
    J Cohen; Fiscal Year: 2004
    ..the depsipeptide most often associated with food poisoning, is unknown, but it is structurally similar to valinomycin, a depsipeptide potassium ionophore...
  5. BINDING, TRANSPORT OF CALCIUM AND POTASSIUM IONOPHORES
    Duncan Haynes; Fiscal Year: 1980
    ..fluorescence decay functions of the Ca2 ion ionophores X537A and A23187 and the K ion ionophore dansyl-lysine-valinomycin will be studied in phospholipid vesicle membranes and in organic solvents to establish the "effective polarity" ..
  6. Assembly & Conductance of Fo Sector of E coli H+-ATPase
    WILLIAM BRUSILOW; Fiscal Year: 2004
    ..into liposomes, and the single-channel F0 proton flux will by measured after the formation of a potassium/valinomycin-induced electrical gradient...
  7. Modulation of NHE Activity by EPEC Infection
    Pradeep Dudeja; Fiscal Year: 2006
    ..Our findings may aid in the future development of improved therapeutic modalities for infectious diarrhea. [unreadable] [unreadable] [unreadable]..
  8. Function and regulation of cardiac Na transporters
    Donald Hilgemann; Fiscal Year: 2008
    ..4) In parallel with the experimental program, we will develop improved mathematical models of cardiac ion homeostasis with an emphasis on these three Na transporters. ..
  9. Volume Regulatory Pathways in Cystic Fibrosis Mice
    Won Kyoo Cho; Fiscal Year: 2003
    ..abstract_text> ..
  10. Antibiotics Discovery from the Great Lakes
    Yi Qiang Cheng; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  11. Effects of Glycosylation on RBC Ca2+ Pump in Diabetes
    ROBERT BOOKCHIN; Fiscal Year: 2006
    ..amounts among normal RBCs ("CVres" RBCs: resistant to dehydration when K-permeabilized by [Ca2+ + A23187] or Valinomycin), also had a high %Hb A1c, suggesting that they represent senescent RBCs; and (iii) that CVres RBCs show loss ..
  12. RBC Ion Transporters as Hemoglobinopathy Risk Modifiers
    Seth Alper; Fiscal Year: 2009
    ..The proposed experiments will increase understanding of sickle cell disease and thalassemia by providing mouse models for genetic tests of new drug therapies under development for near-term clinical testing. ..
  13. MOLECULAR PHYSIOLOGY OF BAND 3 LIKE PROTEINS OF KIDNEY
    Seth Alper; Fiscal Year: 2007
    ..5. Define aspects of transcriptional and translational regulation of AE gene products in kidney of mutant and parental mouse strains and in cultured kidney cells. ..
  14. Sickle Red Cell K+ Transporter Genetics in S. cerevisiae
    Seth Alper; Fiscal Year: 2002
    ..3) (Provisional Aim): Time permitting, to improve, validate, and standardize growth rescue of trk1delta/trk2delta S. cerevisiae by expression in nonpermissive conditions of cDNA encoding the mammalian IK1 K(ca) channel. ..
  15. Structure Function Analysis of TB Pyrazinamidase
    Ying Zhang; Fiscal Year: 2005
    ..These studies will provide important information about mechanisms of PZA action and resistance and may help design new antituberculosis drugs. ..
  16. SICKLING MECHANISMS AND RED CELL MEMBRANES
    ROBERT BOOKCHIN; Fiscal Year: 2002
    ..Once characterized, these will serve investigations within the above three aims. ..
  17. ION CHANNEL REGULATION BY THE CYTOPLASMIC TAIL OF PDK1
    Seth Alper; Fiscal Year: 2005
    ..We will search for novel interacting proteins, and prepare protein suitable for structural analysis. ..
  18. BILIARY SECRETORY PATHWAYS IN CYSTIC FIBROSIS
    Won Kyoo Cho; Fiscal Year: 2003
    ..abstract_text> ..
  19. Release of Apoptogenic Proteins from Brain Mitochondria
    Nickolay Brustovetsky; Fiscal Year: 2009
    ..abstract_text> ..