gels

Summary

Summary: Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid phase liquefies; the resulting colloid is called a sol.

Top Publications

  1. pmc In vitro and ex vivo testing of tenofovir shows it is effective as an HIV-1 microbicide
    Lisa C Rohan
    Magee Womens Research Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 5:e9310. 2010
  2. pmc Complete protection from repeated vaginal simian-human immunodeficiency virus exposures in macaques by a topical gel containing tenofovir alone or with emtricitabine
    Urvi M Parikh
    Laboratory Branch, Division of HIV AIDS Prevention, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
    J Virol 83:10358-65. 2009
  3. ncbi Nonlinear elasticity in biological gels
    Cornelis Storm
    Department of Physics and Astronomy, University of Pennsylvania, 209 South 33rd Street, Philadelphia, Pennsylvania 19104, USA
    Nature 435:191-4. 2005
  4. pmc Prevention of SIV rectal transmission and priming of T cell responses in macaques after local pre-exposure application of tenofovir gel
    Martin Cranage
    Centre for Infection, Division of Cellular and Molecular Medicine, St George s University of London, London, United Kingdom
    PLoS Med 5:e157; discussion e157. 2008
  5. pmc Substrate compliance versus ligand density in cell on gel responses
    Adam Engler
    School of Engineering and Applied Science, Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Biophys J 86:617-28. 2004
  6. ncbi Long-term stable fibrin gels for cartilage engineering
    Daniela Eyrich
    Department of Pharmaceutical Technology, University of Regensburg, Universitaetsstrasse 31, 93051 Regensburg, Germany
    Biomaterials 28:55-65. 2007
  7. ncbi Fibroblast invasive migration into fibronectin/fibrin gels requires a previously uncharacterized dermatan sulfate-CD44 proteoglycan
    Richard A F Clark
    Department of Dermatology, School of Medicine, SUNY at Stony Brook, New York, 11794 8165, USA
    J Invest Dermatol 122:266-77. 2004
  8. ncbi Hydrogels for tissue engineering: scaffold design variables and applications
    Jeanie L Drury
    Department of Biologic and Materials Science, University of Michigan, Room 5210, Ann Arbor, MI 48109, USA
    Biomaterials 24:4337-51. 2003
  9. ncbi Angiogenic sprouting and capillary lumen formation modeled by human umbilical vein endothelial cells (HUVEC) in fibrin gels: the role of fibroblasts and Angiopoietin-1
    Martin N Nakatsu
    Department of Molecular Biology and Biochemistry, 3219 McGaugh Hall, University of California, Irvine, Irvine, CA 92697, USA
    Microvasc Res 66:102-12. 2003
  10. pmc Solution-state 2D NMR of ball-milled plant cell wall gels in DMSO-d(6)/pyridine-d(5)
    Hoon Kim
    Department of Biochemistry, and DOE Great Lakes BioEnergy Research Center, University of Wisconsin, Madison, Wisconsin 53706, USA
    Org Biomol Chem 8:576-91. 2010

Detail Information

Publications424 found, 100 shown here

  1. pmc In vitro and ex vivo testing of tenofovir shows it is effective as an HIV-1 microbicide
    Lisa C Rohan
    Magee Womens Research Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 5:e9310. 2010
    ..To validate our findings with on-going clinical trial results, we evaluated topical tenofovir gel for safety and efficacy. We also modeled systemic application of tenofovir for efficacy...
  2. pmc Complete protection from repeated vaginal simian-human immunodeficiency virus exposures in macaques by a topical gel containing tenofovir alone or with emtricitabine
    Urvi M Parikh
    Laboratory Branch, Division of HIV AIDS Prevention, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
    J Virol 83:10358-65. 2009
    New-generation gels that deliver potent antiretroviral drugs against human immunodeficiency virus type 1 have renewed hopes for topical prophylaxis as a prevention strategy...
  3. ncbi Nonlinear elasticity in biological gels
    Cornelis Storm
    Department of Physics and Astronomy, University of Pennsylvania, 209 South 33rd Street, Philadelphia, Pennsylvania 19104, USA
    Nature 435:191-4. 2005
    ..Unlike simple polymer gels, many biological materials--including blood vessels, mesentery tissue, lung parenchyma, cornea and blood clots--..
  4. pmc Prevention of SIV rectal transmission and priming of T cell responses in macaques after local pre-exposure application of tenofovir gel
    Martin Cranage
    Centre for Infection, Division of Cellular and Molecular Medicine, St George s University of London, London, United Kingdom
    PLoS Med 5:e157; discussion e157. 2008
    ....
  5. pmc Substrate compliance versus ligand density in cell on gel responses
    Adam Engler
    School of Engineering and Applied Science, Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Biophys J 86:617-28. 2004
    ..are found to spread and organize their cytoskeleton and focal adhesions much more so on "rigid" glass or "stiff" gels than on "soft" gels...
  6. ncbi Long-term stable fibrin gels for cartilage engineering
    Daniela Eyrich
    Department of Pharmaceutical Technology, University of Regensburg, Universitaetsstrasse 31, 93051 Regensburg, Germany
    Biomaterials 28:55-65. 2007
    ..Only fibrin gels with a final fibrinogen concentration of 25mg/ml or higher, a Ca(2+) concentration of 20mm and a pH between 6...
  7. ncbi Fibroblast invasive migration into fibronectin/fibrin gels requires a previously uncharacterized dermatan sulfate-CD44 proteoglycan
    Richard A F Clark
    Department of Dermatology, School of Medicine, SUNY at Stony Brook, New York, 11794 8165, USA
    J Invest Dermatol 122:266-77. 2004
    ..We found that CD44H, the non-spliced isoform of CD44, was necessary for fibroblast invasion into fibronectin/fibrin gels. Resting fibroblasts expressed mostly nonglycanated CD44H core protein, which became glycanated with chondroitin ..
  8. ncbi Hydrogels for tissue engineering: scaffold design variables and applications
    Jeanie L Drury
    Department of Biologic and Materials Science, University of Michigan, Room 5210, Ann Arbor, MI 48109, USA
    Biomaterials 24:4337-51. 2003
    ..Consequently, hydrogels have been utilized as scaffold materials for drug and growth factor delivery, engineering tissue replacements, and a variety of other applications...
  9. ncbi Angiogenic sprouting and capillary lumen formation modeled by human umbilical vein endothelial cells (HUVEC) in fibrin gels: the role of fibroblasts and Angiopoietin-1
    Martin N Nakatsu
    Department of Molecular Biology and Biochemistry, 3219 McGaugh Hall, University of California, Irvine, Irvine, CA 92697, USA
    Microvasc Res 66:102-12. 2003
    ..an optimized protocol for human umbilical vein EC in which EC sprout from the surface of beads embedded in fibrin gels. Fibroblast-derived factors, other than Angiopoietin-1, promote sprouting, lumen formation, and long-term stability ..
  10. pmc Solution-state 2D NMR of ball-milled plant cell wall gels in DMSO-d(6)/pyridine-d(5)
    Hoon Kim
    Department of Biochemistry, and DOE Great Lakes BioEnergy Research Center, University of Wisconsin, Madison, Wisconsin 53706, USA
    Org Biomol Chem 8:576-91. 2010
    ..Cell wall gels, produced directly in the NMR tube with perdeutero-dimethylsulfoxide, allowed the acquisition of well resolved/..
  11. doi Sterilizable gels from thermoresponsive block copolymer worms
    Adam Blanazs
    Department of Chemistry, The University of Sheffield, Dainton Building, Brook Hill, Sheffield S3 7HF, UK
    J Am Chem Soc 134:9741-8. 2012
    ..Moreover, this thermo-reversible behavior allows the facile preparation of sterile gels, since ultrafiltration of the diblock copolymer nanoparticles in their low-viscosity spherical form at 4 °C ..
  12. pmc A self-assembly pathway to aligned monodomain gels
    Shuming Zhang
    Department of Materials Science and Engineering, Northwestern University, Evanston, Illinois 60208, USA
    Nat Mater 9:594-601. 2010
    ..the solution of supramolecular filaments can be mixed with cells at physiological temperatures to form monodomain gels of aligned cells and filaments...
  13. doi In the beginning there were soft collagen-cell gels: towards better 3D connective tissue models?
    Robert A Brown
    University College London, UCL Centre for Tissue Regeneration Science, Institute of Orthopaedics, Division of Surgery, RNOH, Stanmore Campus, London, HA7 4LP UK Electronic address
    Exp Cell Res 319:2460-9. 2013
    In the 40 years since Elsdale and Bard's analysis of fibroblast culture in collagen gels we have moved far beyond the concept that such 3D fibril network systems are better models than monolayer cultures...
  14. ncbi Effect of sodium chloride on the gelation temperature, gel strength and bioadhesive force of poloxamer gels containing diclofenac sodium
    C S Yong
    College of Pharmacy, Yeungnam University, 214-1 Dae-dong, Gyongsan 712-749, South Korea
    Int J Pharm 226:195-205. 2001
    ..Furthermore, the poloxamer gels with less than 1...
  15. doi Improved dynamic range of protein quantification in silver-stained gels by modelling gel images over time
    Harald Grove
    Nofima Mat, Norwegian Institute of Food, Fisheries and Aquaculture Research, As, Norway
    Electrophoresis 30:1856-62. 2009
    ..In this paper we take advantage of the fact that silver staining is not an end-point stain by photographing the gels during development...
  16. doi Neurite growth in 3D collagen gels with gradients of mechanical properties
    Harini G Sundararaghavan
    Department of Biomedical Engineering, Rutgers, The State University of New Jersey, USA
    Biotechnol Bioeng 102:632-43. 2009
    ..a microfluidic system to study the response of cells to controlled gradients of mechanical stiffness in 3D collagen gels. An 'H'-shaped, source-sink network was filled with a type I collagen solution, which self-assembled into a ..
  17. doi A poloxamer/chitosan in situ forming gel with prolonged retention time for ocular delivery
    Taís Gratieri
    Department of Pharmaceutical Sciences, University of Sao Paulo, Ribeirao Preto, SP, Brazil
    Eur J Pharm Biopharm 75:186-93. 2010
    ..In conclusion, the in situ forming gel comprised of poloxamer/chitosan is a promising tool for the topical treatment of ocular diseases...
  18. pmc Tensin 2 modulates cell contractility in 3D collagen gels through the RhoGAP DLC1
    Katherine Clark
    Department of Biochemistry, University of Leicester, Leicester, UK
    J Cell Biochem 109:808-17. 2010
    ..We discovered that tensin 2 knockdown significantly reduced the ability of HFFs to contract 3D collagen gels, whilst no effect on fibronectin fibrillogenesis was observed...
  19. pmc Vaginal delivery of the recombinant HIV-1 clade-C trimeric gp140 envelope protein CN54gp140 within novel rheologically structured vehicles elicits specific immune responses
    Rhonda M Curran
    The School of Pharmacy, The Queen s University of Belfast, Belfast, BT9 7BL, Northern Ireland, UK
    Vaccine 27:6791-8. 2009
    Rheologically structured vehicle (RSV) gels were developed as delivery systems for vaginal mucosal vaccination with an HIV-1 envelope glycoprotein (CN54gp140)...
  20. ncbi Active gels: dynamics of patterning and self-organization
    F Backouche
    Department of Chemical Engineering, Ben Gurion University of the Negev, Beer Sheva, Israel
    Phys Biol 3:264-73. 2006
    ..These results may suggest that tuning the assembly and disassembly of cytoskeletal structures can be obtained by tuning the local myosin II concentration/activity...
  21. ncbi Limited efficacy of alcohol-based hand gels
    Axel Kramer
    Institute for Hygiene and Environmental Medicine, University of Greifswald, Greifswald, Germany
    Lancet 359:1489-90. 2002
    Alcohol-based gels have been introduced recently in many hospitals worldwide for hand antisepsis. We investigated the antimicrobial efficacy of ten gels and four rinses according to European standards (EN 1500)...
  22. pmc Soft fibrin gels promote selection and growth of tumorigenic cells
    Jing Liu
    Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China
    Nat Mater 11:734-41. 2012
    ..When cultured within these gels, primary human cancer cells or single cancer cells from mouse or human cancer cell lines grew within a few days ..
  23. ncbi AA2500 testosterone gel normalizes androgen levels in aging males with improvements in body composition and sexual function
    C Steidle
    Northeast Indiana Research, Fort Wayne, Indiana 46825, USA
    J Clin Endocrinol Metab 88:2673-81. 2003
    ..Testosterone gel was well tolerated; however, the testosterone patch resulted in a high rate of application site reactions. Overall, AA2500 is an effective, well tolerated treatment for hypogonadism...
  24. ncbi Phase inversion dynamics of PLGA solutions related to drug delivery. Part II. The role of solution thermodynamics and bath-side mass transfer
    K J Brodbeck
    Department of Chemical Engineering, University of Illinois, Urbana 61801, USA
    J Control Release 62:333-44. 1999
    ..An interpretation of these results in terms of a qualitative model for the protein release mechanism is also given...
  25. ncbi A novel in situ gel for sustained drug delivery and targeting
    Sudipta Ganguly
    Department of Pharmacy Sciences, School of Pharmacy and Health Professions, Creighton University, 2500 California Plaza, Omaha, NE 68178, USA
    Int J Pharm 276:83-92. 2004
    ..Inclusion of GMO enhanced the mucoadhesive property of chitosan by three- to sevenfold. This novel in situ gel system can be useful in the sustained delivery of drugs via oral as well as parenteral routes...
  26. ncbi Pharmacokinetics of transdermal testosterone gel in hypogonadal men: application of gel at one site versus four sites: a General Clinical Research Center Study
    C Wang
    Department of Medicine, Harbor UCLA Medical Center and Research and Education Institute, Torrance, California 90509, USA
    J Clin Endocrinol Metab 85:964-9. 2000
    ..Dose-ranging studies are required to determine dosage regimens for T gel application as a replacement therapy in hypogonadal men...
  27. ncbi The effect of different formulations of chlorhexidine in reducing levels of mutans streptococci in the oral cavity: A systematic review of the literature
    Luciana Gazaniga Maia Ribeiro
    Department of Preventive and Social Dentistry, Faculty of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
    J Dent 35:359-70. 2007
    The current study undertakes a systematic review of the literature in order to assess how long different formulations (solutions, gels and varnishes) of chlorhexidine (CHX) reduce the level of mutans streptococci (MS) in the oral cavity.
  28. ncbi Effects of simvastatin gels on murine calvarial bone
    Michael R Thylin
    Department of Surgical Specialties, University of Nebraska Medical Center, College of Dentistry, Lincoln 68583 0757, USA
    J Periodontol 73:1141-8. 2002
    ..The purpose of this study was to test if similar bone stimulation could be induced by 2 single-dose drug delivery systems appropriate to periodontal therapy...
  29. pmc Rheology and confocal reflectance microscopy as probes of mechanical properties and structure during collagen and collagen/hyaluronan self-assembly
    Ya li Yang
    Department of Chemistry, Columbia University, New York, New York 10027, USA
    Biophys J 96:1566-85. 2009
    ..The following parameters are used to describe the self-assembly process in all gels: the crossover time (t(c)), the slope of the growth phase (k(g)), and the arrest time (t(a))...
  30. ncbi Molecular basis of C(2+)-induced gelation in alginates and pectins: the egg-box model revisited
    I Braccini
    , , CNRS, BP 53 X, 38041 Grenoble Cedex, France
    Biomacromolecules 2:1089-96. 2001
    For many ionic polysaccharides, the ability to form gels in the presence of divalent cations such as calcium is the key to biological functions and technological applications...
  31. doi Silver nanoparticles in therapeutics: development of an antimicrobial gel formulation for topical use
    Jaya Jain
    Centre for Nanobioscience, Agharkar Research Institute, G G Agarkar Road, Pune 411 004, India
    Mol Pharm 6:1388-401. 2009
    ..These results clearly indicate that silver nanoparticles could provide a safer alternative to conventional antimicrobial agents in the form of a topical antimicrobial formulation...
  32. doi Influence of chondroitin sulfate on collagen gel structure and mechanical properties at physiologically relevant levels
    Kate Stuart
    Purdue University, Biomedical Engineering, 206 Martin Jischke Drive, West Lafayette, Indiana 47907, USA
    Biopolymers 89:841-51. 2008
    ..The results of this study demonstrate that collagen organization can be modified by the addition of GAG, leading to altered matrix mechanical properties...
  33. doi Enhanced bioavailability of lacidipine via microemulsion based transdermal gels: formulation optimization, ex vivo and in vivo characterization
    Ramesh Gannu
    National Facilities in Engineering and Technology with Industrial Collaboration NAFETIC Centre, University College of Pharmaceutical Sciences, Kakatiya University, Warangal 506 009, Andhra Pradesh, India
    Int J Pharm 388:231-41. 2010
    ..Microemulsion based transdermal therapeutic system of LCDP was developed and optimized using Box-Behnken statistical design and could provide an effective treatment in the management of hypertension...
  34. ncbi Glucocorticoids and TGF-beta1 synergize in augmenting fibroblast mediated contraction of collagen gels
    F Q Wen
    Department of Internal Medicine, University of Nebraska Medical Center, Omaha 68198-5125, USA
    Inflammation 25:109-17. 2001
    ..Both TGF-beta and glucocorticoids have been reported to increase fibroblast contraction of native collagen gels, a model of fibrotic tissue remodeling...
  35. ncbi Effect of sodium chloride on the release, absorption and safety of diclofenac sodium delivered by poloxamer gel
    Young Joon Park
    College of Pharmacy, Seoul National University, San 56 1, Shinlim Dong, Kwanak Ku, Seoul 151 742, South Korea
    Int J Pharm 263:105-11. 2003
    ..prepared with poloxamers and sodium chloride were previously reported to undergo a phase transition to bioadhesive gels at body temperature...
  36. pmc Fibrin gels and their clinical and bioengineering applications
    Paul A Janmey
    Department of Physiology, Institute for Medicine and Engineering, University of Pennsylvania, 3340 Smith Walk, Philadelphia, PA 19104, USA
    J R Soc Interface 6:1-10. 2009
    Fibrin gels, prepared from fibrinogen and thrombin, the key proteins involved in blood clotting, were among the first biomaterials used to prevent bleeding and promote wound healing...
  37. pmc Postcoital bioavailability and antiviral activity of 0.5% PRO 2000 gel: implications for future microbicide clinical trials
    Marla J Keller
    Department of Medicine, Albert Einstein College of Medicine, Bronx, New York, USA
    PLoS ONE 5:e8781. 2010
    ..This study compared the biological activity and concentration of PRO 2000 in cervicovaginal lavage (CVL) collected in the absence or following coitus...
  38. ncbi Endothelial tubulogenesis within fibrin gels specifically requires the activity of membrane-type-matrix metalloproteinases (MT-MMPs)
    Marc A Lafleur
    School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK
    J Cell Sci 115:3427-38. 2002
    ..This suggests that MMP-2 and other soluble MMPs are not essential for tubulogenesis in fibrin gels, instead TIMP-1-insensitive MMPs, such as members of the membrane type-MMPs (MT-MMP) sub-group (MT1-, MT2-, MT3- or ..
  39. pmc Structure of lipid bilayers
    J F Nagle
    Department of Physics, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    Biochim Biophys Acta 1469:159-95. 2000
    ..The uncertainty in structural results for lipid bilayers is being reduced and best current values are provided for bilayers of five lipids...
  40. doi Macroscopic self-assembly through molecular recognition
    Akira Harada
    Department of Macromolecular Science, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    Nat Chem 3:34-7. 2011
    ..Acrylamide-based gels functionalized with either host (cyclodextrin) rings or small hydrocarbon-group guest moieties were synthesized...
  41. ncbi Zymography: a single-step staining method for quantitation of proteolytic activity on substrate gels
    T M Leber
    Imperial Cancer Research Fund London, United Kingdom
    Anal Biochem 249:24-8. 1997
    ..This improved protocol is fast and reproducible. Its linear range of almost two log scales permits assay of proteolytic activity in a wider range than current methods...
  42. pmc MMP-9/gelatinase B is a key regulator of growth plate angiogenesis and apoptosis of hypertrophic chondrocytes
    T H Vu
    Department of Anatomy, University of California, San Francisco 94143, USA
    Cell 93:411-22. 1998
    ..Growth plates from gelatinase B-null mice in culture show a delayed release of an angiogenic activator, establishing a role for this proteinase in controlling angiogenesis...
  43. doi Induction of spontaneous hyaline cartilage regeneration using a double-network gel: efficacy of a novel therapeutic strategy for an articular cartilage defect
    Nobuto Kitamura
    Department of Sports Medicine and Joint Surgery, Hokkaido University, Kita ku, Sapporo, 060 8638, Japan
    Am J Sports Med 39:1160-9. 2011
    ..A double-network (DN) gel, which was composed of poly-(2-acrylamido-2-methylpropanesulfonic acid) and poly-(N,N'-dimetyl acrylamide) (PAMPS/PDMAAm), has the potential to induce chondrogenesis both in vitro and in vivo...
  44. ncbi Effectiveness of COL-1492, a nonoxynol-9 vaginal gel, on HIV-1 transmission in female sex workers: a randomised controlled trial
    Lut Van Damme
    Institute of Tropical Medicine, Antwerp, Belgium
    Lancet 360:971-7. 2002
    ..Results of studies of its effectiveness in prevention of HIV-1 infection in women have been inconclusive. We aimed to assess effectiveness of this vaginal gel...
  45. ncbi Semisolid systems containing propolis for the treatment of periodontal disease: in vitro release kinetics, syringeability, rheological, textural, and mucoadhesive properties
    Marcos L Bruschi
    Programa de Pós Graduação em Ciências Farmacêuticas, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Av Zeferino Vaz, S N, CEP 14040 903, Ribeirao Preto, SP, Brazil
    J Pharm Sci 96:2074-89. 2007
    ..The data obtained in these formulations indicate a potentially useful role in the treatment of periodontitis and suggest they are worthy of clinical evaluation...
  46. ncbi Safety and acceptability of the candidate microbicide Carraguard in Thai Women: findings from a Phase II Clinical Trial
    Peter H Kilmarx
    Thai Ministry of Public Health US Centers for Disease Control and Prevention Collaboration, Bangkok, Thailand
    J Acquir Immune Defic Syndr 43:327-34. 2006
    ..To determine the safety and acceptability of vaginal application of Carraguard, a carrageenan-derived candidate microbicide gel...
  47. pmc Absence of filamin A prevents cells from responding to stiffness gradients on gels coated with collagen but not fibronectin
    Fitzroy J Byfield
    Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Biophys J 96:5095-102. 2009
    ..Here we use microfluidics techniques to produce gels with a gradient of stiffness to show the essential function of filamin A in cell responses to mechanical stimuli ..
  48. ncbi PRO 2000 gel inhibits HIV and herpes simplex virus infection following vaginal application: a double-blind placebo-controlled trial
    Marla J Keller
    Department of Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Infect Dis 193:27-35. 2006
    ..This study was designed to determine the antiviral activity in cervicovaginal lavage (CVL) samples collected after intravaginal application of 0.5% PRO 2000 gel (Indevus)...
  49. pmc Image-based multiscale modeling predicts tissue-level and network-level fiber reorganization in stretched cell-compacted collagen gels
    Edward A Sander
    Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 106:17675-80. 2009
    ....
  50. doi Micro-structured materials and mechanical cues in 3D collagen gels
    James B Phillips
    Department of Life Sciences, The Open University, Milton Keynes, UK
    Methods Mol Biol 695:183-96. 2011
    Collagen gels provide a versatile and widely used substrate for three-dimensional (3D) cell culture...
  51. doi Design of tenofovir-UC781 combination microbicide vaginal gels
    Patrick F Kiser
    Department of Bioengineering, University of Utah, Salt Lake City, Utah 84112 5820, USA
    J Pharm Sci 101:1852-64. 2012
    ..Experiments and computations led to the specification of three different gels referred to as a spreading gel (SG), an intermediate spreading gel (ISG), and a bolus gel (BG)...
  52. doi RapidArc treatment verification in 3D using polymer gel dosimetry and Monte Carlo simulation
    Sofie Ceberg
    Department of Medical Radiation Physics, Lund University, Skane University Hospital, Malmo, Sweden
    Phys Med Biol 55:4885-98. 2010
    ..Using MC simulations and gel measurements, this verification study successfully demonstrated that the RapidArc plan was both accurately calculated and delivered as planned...
  53. doi Chondrogenic potential of stem cells derived from amniotic fluid, adipose tissue, or bone marrow encapsulated in fibrin gels containing TGF-β3
    Ji Sun Park
    Department of Biomedical Science, College of Life Science, CHA University 606 16, Yeoksam 1 dong, Gangnam Gu, Seoul 135 081, Republic of Korea
    Biomaterials 32:8139-49. 2011
    ....
  54. doi Plastic compression of a collagen gel forms a much improved scaffold for ocular surface tissue engineering over conventional collagen gels
    Shengli Mi
    School of Pharmacy, University of Reading, Reading, RG6 6UB, United Kingdom
    J Biomed Mater Res A 95:447-53. 2010
    We compare the use of plastically compressed collagen gels to conventional collagen gels as scaffolds onto which corneal limbal epithelial cells (LECs) are seeded to construct an artificial corneal epithelium...
  55. doi Integrins on neutrophils are dispensable for migration into three-dimensional fibrin gels
    Leo Koenderman
    Department of Respiratory Medicine, University Medical Center Utrecht, Utrecht, The Netherlands
    Thromb Haemost 104:599-608. 2010
    ..Our study shows that integrins are dispensable for migration on 2D and in 3D fibrin matrices, both when neutrophils enter into the fibrin matrix and when captured in the matrix...
  56. pmc First phase 1 double-blind, placebo-controlled, randomized rectal microbicide trial using UC781 gel with a novel index of ex vivo efficacy
    Peter A Anton
    Center for HIV Prevention Research, UCLA AIDS Institute, Department of Medicine, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, California, United States of America
    PLoS ONE 6:e23243. 2011
    ..A first-in-Phase 1 assessment of preliminary pharmacodynamics was included by measuring changes in ex vivo HIV-1 suppression in rectal biopsy tissue after exposure to product in vivo...
  57. doi Development and characterization of effective topical liposomal system for localized treatment of cutaneous candidiasis
    Madhu Gupta
    Drug Delivery Research Laboratory, Department of Pharmaceutical Sciences, Dr H S Gour Vishwavidyalaya, Sagar, India
    J Liposome Res 20:341-50. 2010
    ....
  58. ncbi Reversible molecular adsorption based on multiple-point interaction by shrinkable gels
    T Oya
    Department of Physics and Center for Materials Science and Engineering, Department of Chemistry, George R Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 286:1543-5. 1999
    A general approach is presented for creating polymer gels that can recognize and capture a target molecule by multiple-point interaction and that can reversibly change their affinity to the target by more than one order of magnitude...
  59. doi The mechanics and fluctuation spectrum of active gels
    Alex J Levine
    Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, USA
    J Phys Chem B 113:3820-30. 2009
    ..In addition, they may form the basis for theoretical studies of biomimetic nonequilibrium gels whose mechanical properties are tunable through the control of their nonequilibrium steady-state.
  60. doi Different approaches for improving skin accumulation of topical corticosteroids
    Taner Senyiğit
    Ege University, Faculty of Pharmacy, Department of Pharmaceutical Technology, Izmir, Turkey
    Int J Pharm 380:155-60. 2009
    ..The formulations tested were chitosan gels, sodium-deoxycholate gels and commercial creams of CP and MF...
  61. pmc Strain-induced alignment in collagen gels
    David Vader
    School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, United States of America
    PLoS ONE 4:e5902. 2009
    ..However, crosslinked networks display similar fiber alignment and the same geometrical properties as uncrosslinked gels, but with full reversibility. Plasticity is therefore not required to align fibers...
  62. pmc Nonlinear strain stiffening is not sufficient to explain how far cells can feel on fibrous protein gels
    Mathilda S Rudnicki
    Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, Massachusetts, USA
    Biophys J 105:11-20. 2013
    ..In this work, we systematically investigate the distance fibroblasts can sense a rigid boundary through fibrous gels by quantifying the spread areas of human lung fibroblasts and 3T3 fibroblasts cultured on sloped collagen and ..
  63. pmc Determination of elastic moduli of thin layers of soft material using the atomic force microscope
    Emilios K Dimitriadis
    Division of Bioengineering and Physical Science, Office of Research Services, Office of the Director, National Institute of Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892 USA
    Biophys J 82:2798-810. 2002
    ..The theoretical and experimental results are compared to macroscopic measurements on poly(vinyl-alcohol) gels.
  64. doi Anion-responsive supramolecular gels
    Hiromitsu Maeda
    College of Pharmaceutical Sciences, Institute of Science and Engineering, Ritsumeikan University, Kusatsu 525 8577, Japan
    Chemistry 14:11274-82. 2008
    Supramolecular gels that change their state or structure in response to anion stimuli have been highlighted...
  65. doi Characterization of type I collagen gels modified by glycation
    Megan E Francis-Sedlak
    Pritzker Institute of Biomedical Science and Engineering, Department of Biomedical Engineering, Illinois Institute of Technology, Chicago, IL 6063, USA
    Biomaterials 30:1851-6. 2009
    ..This study investigates the effects of glycation on the properties of type I collagen gels. Incubation with glucose-6-phopshate (G6P), a reducing sugar that exhibits similar but more rapid glycation than ..
  66. doi Crystal growth of proteins, nucleic acids, and viruses in gels
    Bernard Lorber
    Architecture et Reactivite de l ARN, Universite de Strasbourg, CNRS, IBMC, 15 rue Rene Descartes, 67084 Strasbourg, France
    Prog Biophys Mol Biol 101:13-25. 2009
    ..Chemical gels (like polysiloxane) and physical gels (such as agarose) provide such an environment and are therefore suitable for ..
  67. doi Thermosensitive and mucoadhesive in situ gel based on poloxamer as new carrier for rectal administration of nimesulide
    Yuan Yuan
    Department of Pharmacy, Logistic University of Chinese People s Armed Police Forces, China
    Int J Pharm 430:114-9. 2012
    Poloxamer 407 has excellent thermo-sensitive gelling properties. Nevertheless, these gels possess inadequate poor bioadhesiveness and high permeability to water, which limited its' application as a thermoresponsive matrix...
  68. ncbi Tissue-engineered human skin substitutes developed from collagen-populated hydrated gels: clinical and fundamental applications
    F A Auger
    Departement de Chirurgie, Universite Laval, Quebec, Canada
    Med Biol Eng Comput 36:801-12. 1998
    ..The paper reviews the development of collagen-populated hydrated gels for their eventual use as a therapeutic option for the treatment of burn patients or chronic wounds: tools for ..
  69. ncbi Dosimetric evaluation of a novel polymer gel dosimeter for proton therapy
    O A Zeidan
    M D Anderson Cancer Center Orlando, Orlando, Florida 32806, USA
    Med Phys 37:2145-52. 2010
    ..The aim of this study is to evaluate the dosimetric performance of a newly developed proton-sensitive polymer gel formulation for proton therapy dosimetry...
  70. doi Chlorhexidine gel versus aqueous for preventive use on umbilical stump: a randomized noninferiority trial
    Stephen Hodgins
    Maternal Child Health Integrated Program John Snow Inc, Washington, DC, USA
    Pediatr Infect Dis J 29:999-1003. 2010
    ..Care-takers may prefer gel, resulting in better compliance when implemented at large scale. We examined whether a guar-gum-thickened formulation was at least as efficacious as aqueous in reducing periumbilical flora...
  71. ncbi The bacterial peptide N-formyl-Met-Leu-Phe inhibits killing of Staphylococcus epidermidis by human neutrophils in fibrin gels
    Yongmei Li
    Department of Physiology and Cellular Biophysics, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    J Immunol 168:816-24. 2002
    ..and killing of bacteria in an environment similar to that found in inflamed tissues in vivo, we have used fibrin gels. Fibrin gels (1500 microm thick) containing Staphylococcus epidermidis were formed in Boyden-type chemotaxis ..
  72. doi Preparation of dispersed particle gel (DPG) through a simple high speed shearing method
    Caili Dai
    State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Qingdao 266580, Shandong, China
    Molecules 17:14484-9. 2012
    ..5 μm. A possible mechanism for the formation of DPG has been put forward and discussed in details. The high speed shearing method is considered to be the simple and rapid method for the preparation of DPG...
  73. ncbi Preparation of in situ-forming ophthalmic gels of ciprofloxacin hydrochloride for the treatment of bacterial conjunctivitis: in vitro and in vivo studies
    Naseem A Charoo
    Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University, New Delhi 110062, India
    J Pharm Sci 92:407-13. 2003
    ..Drug concentration in aqueous humor was greater than the minimum inhibitory concentration (MIC 90) against selected microorganisms. The shelf-life of the product was >2 years...
  74. doi Solution densities and estimated total protein contents associated with inappropriate flotation of separator gel in different blood collection tubes
    Ronald C Faught
    Clinical Laboratory, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    Arch Pathol Lab Med 135:1081-4. 2011
    ..In automated analytical systems, gel flotation can lead to mechanical disruption and potential inaccurate result reporting upon aspiration into instrument sampling probes...
  75. ncbi Brachytherapy reirradiation with hyaluronate gel injection of paraaortic lymphnode metastasis of pancreatic cancer: paravertebral approach--a technical report with a case
    Kazushi Kishi
    Department of Radiation Oncology, Tumor Center, Wakayama Medical University, Wakayama City, 641 8510 Japan
    J Radiat Res 52:840-4. 2011
    ..6 U/mL (normal range < 37.5). Conclusively, the brachytherapy with the HGI procedure by paravertebral approach was thought safe and effective in reirradiation of PALN recurrence...
  76. doi Effect of fluoride varnish and gel on dental erosion in primary and permanent teeth
    Christiana Murakami
    Department of Orthodontics and Pediatric Dentistry, School of Dentistry, University of Sao Paulo, Av Lineu Prestes, 2227, 05508 000 Sao Paulo, SP, Brazil
    Arch Oral Biol 54:997-1001. 2009
    ..To assess the effect of a fluoride varnish and gel on the erosive wear of primary and permanent teeth...
  77. ncbi Examination of the flow rheological and textural properties of polymer gels composed of poly(methylvinylether-co-maleic anhydride) and poly(vinylpyrrolidone): rheological and mathematical interpretation of textural parameters
    David S Jones
    School of Pharmacy, The Queen s University of Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast, BT9 7BL, Northern Ireland, United Kingdom
    J Pharm Sci 91:2090-101. 2002
    ..Aqueous gels (pH 7) were prepared containing 15% w/w poly(methylvinylether-co-maleic anhydride) and poly(vinylpyrrolidone) (PVP) ..
  78. pmc Unforeseen decreases in dissolved oxygen levels affect tube formation kinetics in collagen gels
    Hasan Erbil Abaci
    Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences Oncology Center, The Johns Hopkins University, Baltimore, MD 21218, USA
    Am J Physiol Cell Physiol 301:C431-40. 2011
    ..In this study, we hypothesize that dissolved oxygen (DO) levels in collagen gels change during the three-dimensional (3D) culture of human umbilical vein endothelial cells (HUVECs) in atmospheric ..
  79. pmc Ferrihydrite containing gel for chemical imaging of labile phosphate species in sediments and soils using diffusive gradients in thin films
    Jakob Santner
    Rhizosphere Ecology and Biogeochemistry Group, Department of Forest and Soil Sciences, University of Natural Resources and Applied Life Sciences, Peter Jordan Strasse 82, 1190 Vienna, Austria
    Anal Chem 82:7668-74. 2010
    ..The effects of pH, ionic strength and storage time of gels on phosphate binding were investigated and the kinetics of binding and the maximum binding capacity were determined...
  80. doi Dexamethasone eye drops containing γ-cyclodextrin-based nanogels
    Maria D Moya-Ortega
    Faculty of Pharmaceutical Sciences, University of Iceland, Hofsvallagata 53, IS 107 Reykjavik, Iceland
    Int J Pharm 441:507-15. 2013
    ..4±7.8 μg/ml after application of Maxidex(®). The dexamethasone nanogel eye drops were well tolerated with no macroscopic signs of irritation, redness or other toxic effects...
  81. doi Cartilaginous constructs using primary chondrocytes from continuous expansion culture seeded in dense collagen gels
    D H Rosenzweig
    Department of Chemical Engineering, McGill University, 3610 University St, Montreal, QC H3A 0C5, Canada
    Acta Biomater 9:9360-9. 2013
    ..for producing mechanically competent cartilage matrix when three-dimensionally seeded in dense collagen gels. Primary chondrocytes, grown either in CE culture or passaged twice on static silicone dishes (SS culture; ..
  82. ncbi The effect of carbohydrate gels on gastrointestinal tolerance during a 16-km run
    Beate Pfeiffer
    School of Sport and Exercise Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
    Int J Sport Nutr Exerc Metab 19:485-503. 2009
    ..75 +/- 0.09 m) completed two 16-km outdoor-runs. In Study 1 gels were administered to provide 1.0 or 1.4 g CHO/min with ad libitum water intake every 3.2 km...
  83. doi Injectable thermally responsive mucoadhesive gel for sustained protein delivery
    Laura Mayol
    Department of Pharmaceutical and Toxicological Chemistry, University of Naples Federico II, via D Montesano, 49, 800131 Naples, Italy
    Biomacromolecules 12:28-33. 2011
    Poloxamer thermoresponsive gels are widely explored in controlled drug delivery. Nevertheless, these gels possess inadequate mechanical properties, poor bioadhesiveness, and high permeability to water...
  84. pmc Increased brain radioactivity by intranasal P-labeled siRNA dendriplexes within in situ-forming mucoadhesive gels
    Ana Paula Perez
    Programa de Nanomedicinas, Departamento de Ciencia y Tecnologia, Universidad Nacional de Quilmes, Roque Saenz Pena 352, Bernal, Buenos Aires, Argentina
    Int J Nanomedicine 7:1373-85. 2012
    ..In situ-forming mucoadhesive gels would increase the residence time of intranasal material, favoring the nose-to-brain delivery...
  85. ncbi Fibrillar beta-lactoglobulin gels: Part 1. Fibril formation and structure
    Walraj S Gosal
    Department of Life Sciences, King s College London, Franklin Wilkins Building, 150 Stamford Street, London SE1 9NN, United Kingdom
    Biomacromolecules 5:2408-19. 2004
    As a prelude to experimental and theoretical work on the mechanical properties of fibrillar beta-lactoglobulin gels, this paper reports the structural characterization of beta-lactoglobulin fibrils by electron and atomic force microscopy (..
  86. doi Anion-switchable supramolecular gels for controlling pharmaceutical crystal growth
    Jonathan A Foster
    Department of Chemistry, Durham University, South Road, Durham DH1 3LE, UK
    Nat Chem 2:1037-43. 2010
    We describe the use of low-molecular-weight supramolecular gels as media for the growth of molecular crystals. Growth of a range of crystals of organic compounds, including pharmaceuticals, was achieved in bis(urea) gels...
  87. ncbi Apatite-forming ability of carboxyl group-containing polymer gels in a simulated body fluid
    M Kawashita
    Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Biomaterials 24:2477-84. 2003
    Carboxymethylated chitin, gellan gum, and curdlan gels were soaked in a simulated body fluid (SBF) having ion concentrations nearly equal to those of human blood plasma...
  88. doi Thermoreversible mucoadhesive ophthalmic in situ hydrogel: Design and optimization using a combination of polymers
    Divyesh H Shastri
    Department of Pharmaceutics, K B Institute of Pharmaceutical Education and Research, Gandhinagar, Gujarat 382023, India
    Acta Pharm 60:349-60. 2010
    ..The quadratic mathematical model developed is applicable to predicting formulations with desired gelation temperature, gel strength, bioadhesion force and drug release properties...
  89. doi The role of vehicle-nanoparticle interactions in topical drug delivery
    Mojgan Moddaresi
    Pharmaceutical Science Division, King s College, London 150 Stamford St, London, SE1 9NH, United Kingdom
    Int J Pharm 400:176-82. 2010
    ....
  90. doi Poly(2-acrylamido-2-methylpropanesulfonic acid) gel induces articular cartilage regeneration in vivo: comparisons of the induction ability between single- and double-network gels
    Munehiro Ogawa
    Department of Sports Medicine and Joint Surgery, Graduate School of Medicine, Hokkaido University, Sapporo, Japan
    J Biomed Mater Res A 100:2244-51. 2012
    ..of the defect treated with PAMPS/PDMAAm DN gel was significantly higher than those treated with PAMPS and PDMAAm SN gels, and the untreated defect (p = 0.0408, p < 0.0001, and p < 0...
  91. doi Clinical evaluation of lasers and sodium fluoride gel in the treatment of dentine hypersensitivity
    Sebnem Dirikan Ipci
    Department of Periodontology, Yeditepe University Dental Faculty, Istanbul, Turkey
    Photomed Laser Surg 27:85-91. 2009
    ..The aim of the present study was to evaluate and compare the efficacy of CO(2) and Er:YAG lasers alone and in combination with topical sodium fluoride (NaF) in the management of dentine hypersensitivity...
  92. doi Effects of topically applied agents on intra-oral wound healing in a rat model: a clinical and histomorphometric study
    H M Hammad
    Department of Oral Medicine and Surgery, Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan
    Int J Dent Hyg 9:9-16. 2011
    ..This study investigated and compared the influence of these agents on the healing of intra-oral excisional wounds with large epithelial and connective tissue defects...
  93. pmc Stimuli-responsive smart gels realized via modular protein design
    Tijana Z Grove
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511, United States
    J Am Chem Soc 132:14024-6. 2010
    Smart gels have a variety of applications, including tissue engineering and controlled drug delivery...
  94. ncbi Duodenal levodopa infusion monotherapy vs oral polypharmacy in advanced Parkinson disease
    D Nyholm
    Department of Neuroscience, Neurology, Uppsala University Hospital, SE 75185 Uppsala, Sweden
    Neurology 64:216-23. 2005
    ..To compare daytime intraduodenal levodopa/carbidopa infusion as monotherapy with individually optimized conventional combination therapies in patients with advanced Parkinson disease (PD) for motor fluctuations and quality of life (QoL)...
  95. doi Application of a spacer gel to optimize three-dimensional conformal and intensity modulated radiotherapy for prostate cancer
    Michael Pinkawa
    Department of Radiation Oncology, RWTH Aachen University, Germany
    Radiother Oncol 100:436-41. 2011
    ..The aim was to evaluate the impact of a spacer gel on the dose distribution, applying three-dimensional conformal (3D CRT) and intensity modulated radiotherapy (IMRT) planning techniques...
  96. doi Ganciclovir ophthalmic gel 0.15%: in acute herpetic keratitis (dendritic ulcers)
    Jamie D Croxtall
    Adis, a Wolters Kluwer Business, Auckland, New Zealand
    Drugs 71:603-10. 2011
    ..Ganciclovir ophthalmic gel 0.15% was generally well tolerated and was associated with a significantly lower incidence of visual disturbances than aciclovir ointment 3% in the phase III trial...
  97. doi Biocompatibility of surfactant-templated polyurea-nanoencapsulated macroporous silica aerogels with plasma platelets and endothelial cells
    Wei Yin
    School of Mechanical and Aerospace Engineering, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    J Biomed Mater Res A 92:1431-9. 2010
    ..These observations suggest that X-aerogels have a suitable biocompatibility toward platelets, plasma, and vascular endothelial cells, and they have potential for use in blood implantable devices...
  98. pmc PLGA-chitosan/PLGA-alginate nanoparticle blends as biodegradable colloidal gels for seeding human umbilical cord mesenchymal stem cells
    Qun Wang
    Department of Chemical and Petroleum Engineering, University of Kansas, Lawrence, Kansas 66047, USA
    J Biomed Mater Res A 96:520-7. 2011
    ..Rheological studies were employed to probe the differences in plasticity and shear sensitivity of colloidal gels. Viability tests of hUCMSCs seeded on the colloidal gels also demonstrated the negligible cytotoxicity of the ..
  99. ncbi Alginate based new materials
    K I Draget
    Department of Biotechnology, Norwegian University of Science and Technology, Trondheim
    Int J Biol Macromol 21:47-55. 1997
    ..The properties of alginate solutions and gels suggest biomedical and pharmaceutical uses...
  100. pmc Boundary stiffness regulates fibroblast behavior in collagen gels
    Jeffrey John
    Department of Biomedical Engineering, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA 01609, USA
    Ann Biomed Eng 38:658-73. 2010
    ..048-0.64 N m(-1)) to tune the boundary stiffness of suspended collagen gels in between the commonly utilized free and fixed conditions (zero and infinite stiffness boundary stiffness)...
  101. doi Tolerability of topical diclofenac sodium 1% gel for osteoarthritis in seniors and patients with comorbidities
    Herbert S B Baraf
    Center for Rheumatology and Bone Research, a division of Arthritis and Rheumatism Associates, PC, Wheaton, Maryland, USA
    Am J Geriatr Pharmacother 10:47-60. 2012
    ..Topical NSAIDs produce lower systemic NSAID exposure compared with oral NSAIDs, offering potential benefits...

Research Grants75

  1. Chymase-Mediated MMP Activation in Ishemia Reperfusion Injury
    LOUIS JOSEPH DELL'ITALIA; Fiscal Year: 2013
    ..After IEF, proteins on agarose gels will be separated using 2D zymography...
  2. Suppression of Ocular Scarring - Controlled Delivery of an ALK-5 Inhibitor
    Hiroshi Nakamura; Fiscal Year: 2013
    ..for ocular application including GFS because of its stable and longer drug release properties compared to other gels. We will use this novel and promising drug delivery system, PEG-PCL-PLA-PCL-PEG thermoreversible gel, in this ..
  3. Click chemistry for novel antimicrobials against periodontal pathogens
    Valery V Fokin; Fiscal Year: 2010
    ..presence of bacteria in the periodontal region offers itself for topical antibiotic treatment, and antibiotic gels have been used with some success...
  4. Endothelial-reactive antibodies: a diagnostic test for Susac's syndrome
    WILLIAM JAMES WALDMAN; Fiscal Year: 2011
    ..Protein targets will then be identified by mass spectrometry following resolution of EC protein extracts on 2-D gels and localization of reactive proteins by immunoblot with patient sera...
  5. 3D Differentiation of Mesenchymal Stem Cells in Peptide Amphiphile Matrices
    John B Matson; Fiscal Year: 2011
    ..Once a Dex-releasing PA has been synthesized, Dex release will be measured from the PA gels into buffer in the absence of cells...
  6. Molecular Regulation of Vascular Regression
    Roberto Nicosia; Fiscal Year: 2013
    ..We have reproduced the process of vascular regression by culturing rat or mouse aortic rings in collagen gels under chemically defined culture conditions...
  7. Environmental and genetic regulation of copy number variation (CNV)
    THOMAS PETES; Fiscal Year: 2010
    ..the strains with CNV events using DNA microarrays and a gel system that separates intact chromosomal DNAs (CHEF gels)...
  8. Development of Cytokine-Inhibiting Gels for Treating Chronic Wounds
    Newell R Washburn; Fiscal Year: 2010
    ..The gels are composed of monoclonal antibodies that neutralize pro-inflammatory cytokines, such as tumor necrosis factor- 1, ..
  9. Phase 2 Study of rAAV1-CB-hAAT for Treatment of Alpha-1 Antitrypsin Deficiency
    Jeffrey D Chulay; Fiscal Year: 2013
    ..of serum concentrations of M-specific AAT and total AAT and serum AAT phenotype determined on isoelectric focusing gels. Additional information to be collected will include presence of the vector in blood or semen, changes in serum ..
  10. CHLORIDE CHANNELS IN LUNG DEVELOPMENT
    Pamela L Zeitlin; Fiscal Year: 2012
    ..HSP90 interacts with ClC-2 and we have developed the technology to identify ClC-2 and its binding partners on 2D gels followed by MALDI-TOF. The goal is to discover the factors that maximize surface ClC-2 expression and function...
  11. Redox Control of Apoptosis Signaling
    MARGARET BRIEHL; Fiscal Year: 2009
    ..2) To use immunoblots and 2D gels with proteomic analyses to identify key differences in mitochondrial proteins between glucocorticoid-sensitive and ..
  12. Biomimetic scaffolds for targeted peripheral nerve regeneration
    DAVID SHREIBER; Fiscal Year: 2009
    ..We will evaluate neurite growth from isolated mouse spinal cord neurons through gels grafted with either HNK-1 or PSA mimics, and then optimize the dual presentation of the mimics in vitro...
  13. Multiplex Fluorescent Zdyes for Differential Glycomic Detection
    EDWARD DRATZ; Fiscal Year: 2009
    ..Most PTMs cause shifts in 2D gel spot positions and thus 2D gels provide global pictures of PTM patterns...
  14. ROLE OF MONONUCLEAR LEUKOCYTES IN IMMUNITY
    Samuel Silverstein; Fiscal Year: 2009
    We have used three-dimensional fibrin and collagen I gels to compare neutrophil (PMN) bactericidal activity in tissue-like environments vs...
  15. HIV integrase/DNA complexes and concerted integration
    Duane P Grandgenett; Fiscal Year: 2010
    ..We recently identified a nucleoprotein complex on native agarose gels where IN non-covalently juxtaposes two viral DNA ends that produces the synaptic complex (SC) that possesses ..
  16. Targeting stromal reaction in pancreatic cancer to overcome chemo-resistance
    Hidayatullah G Munshi; Fiscal Year: 2013
    ..This hypothesis is based on our preliminary data demonstrating that PDAC cels grown in 3D collagen gels have increased histone H3 acetylation;decreased expression of heterochromatin protein 11 (HP11);and are protected ..
  17. Field Validation of New XRF Analyzers: Assessing Personal Exposure to Metals
    PATRICK JEREMY PARSONS; Fiscal Year: 2012
    ..lotions and gels that are applied to the body (makeup, perfume, sun block, antiperspirant, shampoo, soap, toothpaste, and other ..
  18. Tissue Engineered Aortic Heart Valves: Scaffolds and Stem Cells
    DAN TEODOR SIMIONESCU; Fiscal Year: 2013
    ..In Specific Aim 2, we will prepare human mesenchymal stem cells. Stem cells will be then seeded onto collagen gels and subjected to controlled load regimes in a FlexerCell system...
  19. Sex, sex steroids and dry eye syndromes
    David A Sullivan; Fiscal Year: 2013
    ..g. gene chips), real-time-PCR, cell cultures, immunoassays, 2D gels, enzyme assays, histology, image analysis and hormone reconstitution experiments...
  20. Engineering Developmental Microenvironments: Cartilage Formation and Maturation
    Robert L Mauck; Fiscal Year: 2012
    ..These gels, coupled with physiologic loading and temporal application of relevant soluble factors will re-create developmental ..
  21. PTHrP/IHH Microenvironment Control for Growth Plate Tissue Engineering
    JUAN MANUEL TABOAS; Fiscal Year: 2013
    ..uniaxial gradients of fluorescently tagged "idealized" morphogens across poly(ethylene glycol) diacrylate (PEGDA) gels housed within our bioreactor...
  22. Nanocapsule-mediated topical delivery of VLP-generating Dengue DNA vaccine
    Gretchen M Unger; Fiscal Year: 2013
    ..a separate study arm, we will compound the s50 DENV-2 DNA vaccine in a panel of semisolid vehicles (lotions and/or gels) to obtain maximum and uniform dose delivery, and execute a proof-of-principle study in mice with the best ..
  23. Optimizing Therapeutic Revascularization by Endothelial Cell Transplantation
    W Mark Saltzman; Fiscal Year: 2013
    ..the past 4 years we have optimized systems in which primary human endothelial cells (ECs), suspended in protein gels, self assemble into vascular conduits in vitro...
  24. Injectable Anabolic Biomaterials for Bone Regeneration
    LAKSHMI SREEDHARAN NAIR; Fiscal Year: 2013
    ..designed to evaluate in vitro the anti apoptotic, mitogenic and osteogenic effect of recombinant human lactoferrin gels (rhLfG) to rat bone marrow derived mesenchymal stem cells...
  25. Controlled Chemotaxis and Differentiation of Embryonic Cardiac Progenitor Cells
    Gregory M Fomovsky; Fiscal Year: 2013
    ..observation of cell migration, differentiation, and complex organization in the 3D environment of collagen gels. To study the migratory behavior and differentiation potential of Nkx2...
  26. Identification of Binding Proteins of the Xenopus Morphogenetic Factor Tumorhead
    Edwin E Traverso; Fiscal Year: 2012
    ..Proteins that co-purify with TH from embryo cell extracts will be visualized in polyacrylamide gels, and will be identified by mass spectrometry...
  27. Vaginal Ring for Preventing Bacterial Vaginosis
    THOMAS RAY MOENCH; Fiscal Year: 2010
    ..for expecting it to be effective: Lactic acid potently inactivates BV-associated bacteria in vitro, and vaginal gels with lactic acid, when applied frequently over many weeks, have been shown clinically to reduce BV relapse...
  28. Cell-Matrix Interactions in Epithelial Polarization
    Karl S Matlin; Fiscal Year: 2010
    ..and signaling in both two-dimensional cultures on permeable supports and in three-dimensional collagen gels. In Specific Aim 3 we will determine which integrin receptors for laminin are involved in the polarization process ..
  29. Safety of Non-Medicated Intravaginal Rings for Microbicide Delivery
    Marla J Keller; Fiscal Year: 2010
    ..release of hormones for contraception and the difficulties with adherence to coitally-dependent microbicide gels suggest that IVRs may prove to be a superior option for microbicide delivery...
  30. STRUCTURE AND BEHAVIOR OF YEAST TELOMERES
    Virginia A Zakian; Fiscal Year: 2013
    ..monitored by measuring DNA polymerase occupancy and by visualizing replication intermediates with two dimensional gels of telomeric DNA in Rif1-depleted cells...
  31. MULTILAYER VASCULAR GRAFTS BASED ON COLLAGEN-MIMETIC HYDROGELS
    Mariah S Hahn; Fiscal Year: 2013
    ..We propose to circumvent the limitations associated with native biopolymer gels by generating novel bioactive hydrogels using the collagen-mimetic protein Scl2.28 (Scl2)...
  32. Trigeminal Nerve Stimulation for Epilepsy
    Christopher DeGiorgio; Fiscal Year: 2012
    ..1. Evaluate the effects of a series of stimulation parameters and electrode gels in an ISO compliant skin irritation model in rabbits for optimal tolerability of the system for patients. 2...
  33. PHOSPHORYLATION OF GAP JUNCTION PROTEINS
    Paul D Lampe; Fiscal Year: 2013
    ..events is in itself important but given that drugs to affect Cx43-related cardiac function and Cx43 anti-sense gels to speed wound healing are being tested in humans, we need to fully define these medically important biological ..
  34. Emp2: Potential Control Of Pathology In Retinal Pigment Epithelium
    Lynn Gordon; Fiscal Year: 2009
    ..the effect of EMP2 blockade on cell survival, cell signal transduction mechanisms, and ability to contract collagen gels. In addition, this aim tests the prediction that dose synergy with reduced toxicity could be achieved through ..
  35. Suppression of the Immune Response in Spaceflight and Aging
    Millie Hughes-Fulford; Fiscal Year: 2011
    ..3) Analyze the protein synthesis of the upregulated/downregulated target genes (SDS PAGE gels and Western Blot proteomics) that are affected by T-cell activation under normal gravity and microgravity ..
  36. Measurements of BPDE-DNA adducts by solid state nonopore and deep sequencing (PQ
    Farhad Kosari; Fiscal Year: 2013
    ..Such investigations would require laborious techniques involving highly radioactive reagents and sequencing gels. Novel non-intensive methodologies for estimating BPDE concentrations on the genome could provide new tools for ..
  37. Vasculogenesis and hyperoxia in the developing retina.
    GERARD ANTHONY LUTTY; Fiscal Year: 2013
    ..in vivo by immunohistochemistry and in vitro using GC and Muller cells co-cultured with angioblasts in 3-D gels. Coated nanofibers will be used to examine the influence of individual matrix components and guidance molecules (..
  38. Delivery of Particle Vaccines to Control Trafficking Patterns of T Cells
    James Jaehyun Moon; Fiscal Year: 2013
    ..against influenza virus? (2) Can we develop infection- mimicking materials using ICMVs loaded in injectable gels and apply this new vaccine delivery approach to confer long-term memory response in mucosal tissues? The results ..
  39. Evaluation of Recombinant Human Lactoferrin as an Osteogenic Biomaterial
    Ashley A Amini; Fiscal Year: 2013
    ..We propose to first investigate the physical properties and characterize the rhLF gels to be further employed as a regenerative skeletal biomaterial...
  40. Virulence Factors of Francisella tularensis
    Barbara J Mann; Fiscal Year: 2010
    ..DsbA/DsbB pathway protein profiles of the dsbB mutants will be compared to wild-type bacteria using polyacrylamide gels and mass spectrometry techniques. Several substrates will be selected for further study...
  41. Factors that influence susceptibility to acute streptococcal pharyngitis
    Patricia A Ryan; Fiscal Year: 2012
    ..sialic acid, in commercial available preparations of salivary mucin (the major glycoprotein component of mucus gels), and that the binding to sialic acid is also important in adherence to pharyngeal epithelial cells...
  42. Novel Determination of Microbicide PK in Women's Reproductive Health
    DAVID FRANK KATZ; Fiscal Year: 2013
    ..APIs (using confocal multimodal Raman spectroscopy) delivered to luminal fluids and underlying tissues by gels and rings...
  43. Trapping HIV in mucus with IgG antibodies
    SAMUEL LAI; Fiscal Year: 2012
    ..of specific IgG1can trap Herpes Simplex Virus and virus-like particles that otherwise rapidly penetrate mucus gels. Trapping HIV in mucus will likely reduce or block any effective contact between HIV and target cells...
  44. Creating Platforms for the Proteomics and Membrane Proteins
    PAUL CREMER; Fiscal Year: 2012
    ..proteins are removed from their native biomembrane environment in order to perform electrophoretic separation in gels. This can be unsuitable due to protein aggregation...
  45. Auto-Antibodies as Serum Biomarkers for Age-Related Macular Degeneration
    Alessandro Iannaccone; Fiscal Year: 2010
    ..Homogenate proteins reacting against serum auto-Abs will be immunoprecipitated and separated on 2D gels. The identity of the most common gel spots observed uniquely and/or more intensely in AMD samples will be ..
  46. Zosteric Acid Integrated Thermo-reversible Gels for Preventing Surgical Adhesions
    Bi min Newby; Fiscal Year: 2012
    ....
  47. Scleraxis-directed stem cell differentiation for ligament tissue engineering
    LINDA ANN DAHLGREN; Fiscal Year: 2010
    ..stem cell line will be engineered to overexpress scleraxis and cells will be cultured in three-dimensional collagen gels under conditions of static strain (tension)...
  48. SIGNIFICANCE OF GGT EXPRESSION IN TUMORS
    MARIE HANIGAN; Fiscal Year: 2009
    ..carcinoma (HCC) have tumor-specific isoforms of GGT in their serum that can be resolved on native polyacrylamide gels. GGT is a type II transmembrane protein...
  49. Spatiotemporal Delivery of miRNA Anatgomir for Promoting Vascular Self-Assembly
    W Mark Saltzman; Fiscal Year: 2012
    ..that well differentiated human endothelial cells (ECs) will self assemble into vascular conduits in protein gels both in vitro and in vivo after implantation into immunodeficient mouse hosts...
  50. Linking Biological, Optical, and Mechanical Properties in the Airway Mucosa
    STEVEN CARL GEORGE; Fiscal Year: 2012
    ..relationship between optical endpoints and the mechanical properties of both acellular and cellularized collagen gels in which collagen content, microstructure, and transforming growth factor-2 are systematically altered;3) quantify ..
  51. A combined drug and neural stem cell delivery approach for treating stroke
    Kyle J Lampe; Fiscal Year: 2013
    ..This hydrogel has the unique ability to flow as a liquid during injection, but gels to form a scaffold for cell growth once inside the brain...
  52. MOLECULAR PROBES AND METHODS FOR FLUORESCENCE LIFETIME TOMOGRAPHY AND MICROSCOPY
    Samuel Achilefu; Fiscal Year: 2012
    ..Uniquely, OI provides high throughput screening of molecular targets from analytical assays in solution and gels through cells and small animals, and eventual translation to humans...
  53. Nitric oxide eluting therapies for vascular surgery
    MELINA RAE KIBBE; Fiscal Year: 2010
    ..Specific Aim 1: Evaluate the effect of novel NO-eluting gels on the development of neointimal hvperplasia following arterial injury and vein bypass grafting...
  54. Suppression of the Immune Response in Spaceflight and Aging
    Millie Hughes-Fulford; Fiscal Year: 2013
    ..3) Analyze the protein synthesis of the upregulated/downregulated target genes (SDS PAGE gels and Western Blot proteomics) that are affected by T-cell activation under normal gravity and microgravity ..
  55. Injectable, In Situ Forming Hydrogels for Craniofacial Tissue Engineering
    Tiffany Vo; Fiscal Year: 2013
    ..to develop an injectable, in situ forming hydrogel that utilizes a unique dual gelation mechanism to rapidly form gels at physiological temperature, chemically crosslink to maintain hydrogel stability, and biodegrade for full tissue ..
  56. SAM Domains
    James U Bowie; Fiscal Year: 2012
    ..The SAM domains will be screened for aggregate formation by native gels and/or gel filtration chromatography...
  57. Transdermal Cannabinoid Prodrug Treatment for Cannabis Withdrawal and Dependence
    Lynn Webster; Fiscal Year: 2010
    ..Transdermal delivery systems offer a number of improvements over other delivery systems. Patches and gels do not require swallowing, eliminating oral side effects and bitter taste concerns;nor do they require skin ..
  58. Regulation of histone H3 dephosphorylation in breast cancer cells
    PEDRO RODRIGUEZ COLLAZO RODRIGUEZ-COLLAZO; Fiscal Year: 2011
    ..Analysis of histones by TAU and AU-gels. Knowing the mechanisms of how a variety of drugs provoke the lost of phosphate from histones which in turn leads ..
  59. Soft Hydrogels for Expansion of Quiescent Human MSCs
    Sarah Atzet; Fiscal Year: 2010
    ..We will compare MP-attached gels with the gelatin-containing HyStem-C hydrogel to identify conditions that maintain quiescence of mesenchymal stem ..
  60. Novel Biomaterials as Sealants for Applications in Annulus Fibrosus Repair
    Clare Canal Guterl; Fiscal Year: 2012
    ..The two gels proposed are a genipin cross- linked fibrin gel (fib-gen) and carboxymethylcellulose hydrogel (CMC)...
  61. Bioactive Gels that Promote Long-Term Islet Survival and Function
    Kristi S Anseth; Fiscal Year: 2012
    ..The biophysical properties (e.g., crosslinking density) of the gels will be varied, and the relative role of hypoxia will be examined...
  62. Enhanced proteomics capabilities for quantitation of proteins with a triple quad
    KENNETH DONALD GREIS; Fiscal Year: 2010
    ..These include routine analysis of proteins and peptide, traditional proteomics approaches (comparative 2-D gels profiling, image analysis and protein identification by MS), mapping sites of protein modification, and research ..
  63. Development of Topical Neuroinflammatory Inhibitors
    MANDIP SACHDEVA; Fiscal Year: 2005
    ..The specific aims of this research proposal are: Aim #1) The preparation of topical formulations (gels, creams and lotions) of neuromodulatory agents using prototype topical vehicles and screening these formulations ..
  64. Nitric Oxide Releasing Topical Gel for Burn Wound Healing
    ANJAL SHARMA; Fiscal Year: 2007
    ..following exogenous NO has also been reported, including direct application of NO gas or application of topical gels or creams that synthesize NO and other cytotoxic nitrogen oxide species (nitrous acid, NO2)...
  65. Mucus Penetrating Nanoparticles for Vaginal Drug Delivery
    Justin Hanes; Fiscal Year: 2009
    ..Nanoparticles now used for mucosal drug delivery are mucophilic and bind tightly to mucus gels. Unfortunately, these conventional nanoparticles are rapidly cleared with luminal mucus and very few reach the ..
  66. Stimuli Responsive Topical Gels for Mechanically Assisted Wound Debridement
    Aristidis Veves; Fiscal Year: 2010
    ..technology by pursuing the following specific aims, namely (1) prepare and characterize wound debridement gels, (2) develop a comprehensive theoretical model to explain the gel volume phase transition, (3) perform ex- vivo ..
  67. IN SITU POLYMERIZABLE GELS FOR DENTAL TISSUE ENGINEERING
    Antonios Mikos; Fiscal Year: 2002
    ....
  68. DNA-crosslinked gels: New advanced biomaterials
    NOSHIR LANGRANA; Fiscal Year: 2006
    ..Choice of base sequence allows for the design of gels that are degraded by particular restriction endonucleases or by particular messenger RNA strands...
  69. Superior Near-IR Dyes for Bioimaging
    ARUNKUMAR EASWARAN; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Optical imaging is an advanced technique to image biotechnology products like gels, sensors, and micro arrays. It is also used increasingly to monitor specific molecular pathways in animals...
  70. "Smart" Materials to Engineer a More Complete Stem Cell Niche
    Adam Engler; Fiscal Year: 2009
    ..These gels, comprised of hyaluronic acid-co-acrylamide polymer, should present spatially- and temporally-controlled matrix ..
  71. IN VIVO STUDIES OF SALIVA ANTIMICROBIAL PROTEINS
    JOEL RUDNEY; Fiscal Year: 2001
    ..3.) Compare groups by image analysis of native and reducing gels to test the hypothesis that redundancy and complexing will allow salivas with different compositions to exert ..
  72. Bright and Stable Near-IR dyes for Bioimaging
    ARUNKUMAR EASWARAN; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Optical imaging is an advanced technique to image biotechnology products like gels, sensors, and micro arrays. It is also used increasingly to monitor specific molecular pathways in animals...
  73. DISTRIBUTION AND TRANSPORT OF LIPIDS IN MUCIN GELS
    Timothy Wiedmann; Fiscal Year: 2000
    ..physical chemical principles that determine the equilibrium distribution and dynamic properties of lipids in mucous gels. The diffusion coefficients of a series of p-n-alkyl benzoic acid and aniline derivatives will be measured in the ..
  74. Microbubble Infused Hydrogels for Cartilage Tissue Engineering
    Clark T Hung; Fiscal Year: 2012
    ..The microbubble dissolution process generates micropores that are homogeneously distributed within the gels, while enabling the direct ab initio immobilization of cels within the gels...
  75. BIOMEDICAL RESEARCH SUPPORT
    Peter Henson; Fiscal Year: 1991
    ..2-D gels are being widely used at this Medical Center in a broad range of projects, including efforts to understand AIDS, ..