- How mitochondria produce reactive oxygen species
Michael P Murphy
MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB20XY, UK
Biochem J 417:1-13. 2009
- Genomic expression programs in the response of yeast cells to environmental changes
A P Gasch
Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5428, USA
Mol Biol Cell 11:4241-57. 2000
..in transcript levels over time for almost every yeast gene, as cells responded to temperature shocks, hydrogen peroxide, the superoxide-generating drug menadione, the sulfhydryl-oxidizing agent diamide, the disulfide-reducing ..
- Oxidative stress, antioxidants and stress tolerance
Dept of Botany, Plant Sciences Institute, 353 Bessey Hall, Iowa State University, Ames, IA 50011, USA
Trends Plant Sci 7:405-10. 2002
..These raise several intriguing questions about the relationships between ROI signaling, ROI stress and the production and scavenging of ROIs in the different cellular compartments...
- A common mechanism of cellular death induced by bactericidal antibiotics
Michael A Kohanski
Center for BioDynamics and Center for Advanced Biotechnology, Boston University, Boston, MA 02215, USA
Cell 130:797-810. 2007
- A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish
Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02114, USA
Nature 459:996-9. 2009
..Owing to their fast diffusion and versatile biological activities, reactive oxygen species, including hydrogen peroxide (H(2)O(2)), are interesting candidates for wound-to-leukocyte signalling...
- Genetically encoded fluorescent indicator for intracellular hydrogen peroxide
Vsevolod V Belousov
Laboratory of Genes for Regeneration, Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho Maklaya Street 16 10, Moscow 117997, Russia
Nat Methods 3:281-6. 2006
We developed a genetically encoded, highly specific fluorescent probe for detecting hydrogen peroxide (H(2)O(2)) inside living cells...
- Cellular defenses against superoxide and hydrogen peroxide
James A Imlay
Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
Annu Rev Biochem 77:755-76. 2008
..They also developed mechanisms to defend themselves against superoxide (O(2)()) and hydrogen peroxide, partially reduced oxygen species that are generated as inadvertent by-products of aerobic metabolism...
- Global transcriptional responses of fission yeast to environmental stress
The Wellcome Trust Sanger Institute, Cambridge CB10 1SA, United Kingdom
Mol Biol Cell 14:214-29. 2003
..profiles of all known and predicted genes in response to five stress conditions: oxidative stress caused by hydrogen peroxide, heavy metal stress caused by cadmium, heat shock caused by temperature increase to 39 degrees C, osmotic ..
- Mammalian thioredoxin is a direct inhibitor of apoptosis signal-regulating kinase (ASK) 1
Department of Biochemistry, The Cancer Institute, Tokyo, Japanese Foundation for Cancer Research, 1 37 1 Kami Ikebukuro, Toshima ku, Tokyo 170, Japan
EMBO J 17:2596-606. 1998
..Treatment of cells with N-acetyl-L-cysteine also inhibited serum withdrawal-, TNF-alpha- and hydrogen peroxide-induced activation of ASK1 as well as apoptosis...
- Myeloperoxidase: friend and foe
Seymour J Klebanoff
Department of Medicine, University of Washington School of Medicine, Seattle, WA 98195 7185, USA
J Leukoc Biol 77:598-625. 2005
..is one consisting of myeloperoxidase (MPO), released into the phagosome during the degranulation process, hydrogen peroxide (H2O2), formed by the respiratory burst and a halide, particularly chloride...
- Salidroside protects human erythrocytes against hydrogen peroxide-induced apoptosis
Erin Wei Qian
Programme of Biochemistry, School of Life Sciences, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, People s Republic of China
J Nat Prod 75:531-7. 2012
..Compound 1 is a protective agent in human erythrocytes against oxidative stress and may be a good adaptogen to enhance the body's resistance to stress and fatigue...
- Cell signaling. H2O2, a necessary evil for cell signaling
Sue Goo Rhee
Institute of Molecular Life Science and Technology, Ewha Women s University, Seoul 120 750, South Korea
Science 312:1882-3. 2006
- NF-kappaB activation by reactive oxygen species: fifteen years later
Center for Biomedical Integrated Genoproteomics CBIG, Virology and Immunology Unit, University of Liege, 4000 Liege, Belgium
Biochem Pharmacol 72:1493-505. 2006
..Finally, we critically review the recent data highlighting the role of ROS in NF-kappaB activation by proinflammatory cytokines (TNF-alpha and IL-1beta) and lipopolysaccharide (LPS), two major components of innate immunity...
- Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader
Neuroscience Laboratory, USDA ARS, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA 02111, USA
Free Radic Biol Med 27:612-6. 1999
..This method is reliable and efficient for evaluating the potency of pro-oxidants and can be used to evaluate the efficacy of antioxidants against OS in cells...
- Topology of superoxide production from different sites in the mitochondrial electron transport chain
MRC Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, England
J Biol Chem 277:44784-90. 2002
..By measuring hydrogen peroxide production in the absence and presence of exogenous superoxide dismutase, we established the topology of ..
- Mediation of poly(ADP-ribose) polymerase-1-dependent cell death by apoptosis-inducing factor
Seong Woon Yu
Department of Neurology and Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
Science 297:259-63. 2002
..These data support a model in which PARP-1 activation signals AIF release from mitochondria, resulting in a caspase-independent pathway of programmed cell death...
- Hydrogen peroxide regulation of endothelial function: origins, mechanisms, and consequences
Section of Cardiology, Department of Medicine, The Division of Biological Sciences and Pritzker School of Medicine, The University of Chicago, 5841 S Maryland Ave, MC6088, Chicago, IL 60637, USA
Cardiovasc Res 68:26-36. 2005
..O2(*-) rapidly degrades by reacting with nitric oxide (NO*), the O2*- signal preserved by dismutation into hydrogen peroxide (H2O2) exerts prolonged signaling effects...
- Specific aquaporins facilitate the diffusion of hydrogen peroxide across membranes
Gerd P Bienert
Department of Agricultural Sciences, Faculty of Life Science, Copenhagen University, DK 1871 Frederiksberg C, Denmark
J Biol Chem 282:1183-92. 2007
..g. to cross membranes. Here we present evidence that aquaporins can channel hydrogen peroxide (H2O2)...
- Pharmacologic ascorbic acid concentrations selectively kill cancer cells: action as a pro-drug to deliver hydrogen peroxide to tissues
Molecular and Clinical Nutrition Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 102:13604-9. 2005
..These findings give plausibility to i.v. ascorbic acid in cancer treatment, and have unexpected implications for treatment of infections where H(2)O(2) may be beneficial...
- Mitochondrial complex III is required for hypoxia-induced ROS production and cellular oxygen sensing
Robert D Guzy
Department of Medicine, The University of Chicago, Chicago, Illinois 60637, USA
Cell Metab 1:401-8. 2005
..These results demonstrate that mitochondria function as O(2) sensors and signal hypoxic HIF-1 alpha and HIF-2 alpha stabilization by releasing ROS to the cytosol...
- Distinct roles of Nox1 and Nox4 in basal and angiotensin II-stimulated superoxide and hydrogen peroxide production
Sergey I Dikalov
Division of Cardiology, Department of Medicine, Emory University School of Medicine, 1639 Pierce Drive, Atlanta, GA 30322, USA
Free Radic Biol Med 45:1340-51. 2008
NADPH oxidases are major sources of superoxide (O2*-) and hydrogen peroxide (H2O2) in vascular cells...
- Arabidopsis gp91phox homologues AtrbohD and AtrbohF are required for accumulation of reactive oxygen intermediates in the plant defense response
Miguel Angel Torres
Department of Biology and Curriculum in Genetics and Molecular Biology, CB 3280, 108 Coker Hall, University of North Carolina, Chapel Hill, NC 27599 3280, USA
Proc Natl Acad Sci U S A 99:517-22. 2002
..parasitica. Paradoxically, although atrbohD mutation eliminated the majority of total ROI production, atrbohF mutation exhibited the strongest effect on cell death...
- Exposure to hydrogen peroxide induces oxidation and activation of AMP-activated protein kinase
Jaroslaw W Zmijewski
Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294 0012, USA
J Biol Chem 285:33154-64. 2010
..The present findings also imply that AMPK activation, in addition to being a response to alterations in intracellular metabolic pathways, is directly influenced by cellular redox status...
- In vaginal fluid, bacteria associated with bacterial vaginosis can be suppressed with lactic acid but not hydrogen peroxide
Deirdre E O'Hanlon
Mucosal Protection Laboratory, Thomas C Jenkins Department of Biophysics, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
BMC Infect Dis 11:200. 2011
b>Hydrogen peroxide (H2O2) produced by vaginal lactobacilli is generally believed to protect against bacteria associated with bacterial vaginosis (BV), and strains of lactobacilli that can produce H2O2 are being developed as vaginal ..
- Myeloperoxidase associated with neutrophil extracellular traps is active and mediates bacterial killing in the presence of hydrogen peroxide
Department of Pathology, University of Otago Christchurch, 2 Riccarton Ave, Christchurch, New Zealand
J Leukoc Biol 91:369-76. 2012
..Our results show that the enzymatic activity of MPO on NETs could contribute to antimicrobial activity or tissue injury when NETs are released from neutrophils at sites of infection or inflammation...
- Lyn is a redox sensor that mediates leukocyte wound attraction in vivo
Sa Kan Yoo
Program in Cellular and Molecular Biology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
Nature 480:109-12. 2011
..Wounds and tumours--a type of 'unhealed wound'--generate hydrogen peroxide (H(2)O(2)) through an NADPH oxidase (NOX)...
- ABA-induced NO generation and stomatal closure in Arabidopsis are dependent on H2O2 synthesis
Centre for Research in Plant Science, Genomics Research Institute, University of the West of England, UWE, Bristol, Frenchay Campus, Coldharbour Lane, UK
Plant J 45:113-22. 2006
Nitric oxide (NO) and hydrogen peroxide (H(2)O(2)) are key signalling molecules produced in response to various stimuli and involved in a diverse range of plant signal transduction processes...
- Inactivation of a peroxiredoxin by hydrogen peroxide is critical for thioredoxin-mediated repair of oxidized proteins and cell survival
Alison M Day
Institute for Cell and Molecular Biosciences, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, Tyne and Wear, UK
Mol Cell 45:398-408. 2012
..Here we demonstrate that, in cells treated with hydrogen peroxide, the Prx Tpx1 is a major substrate for thioredoxin in the fission yeast Schizosaccharomyces pombe and, as ..
- Duox, Flotillin-2, and Src42A are required to activate or delimit the spread of the transcriptional response to epidermal wounds in Drosophila
Michelle T Juarez
Section of Cell and Developmental Biology, Division of Biology, University of California San Diego, La Jolla, California, United States of America
PLoS Genet 7:e1002424. 2011
..One gene required to activate epidermal wound reporters encodes Dual oxidase, an enzyme that produces hydrogen peroxide. We also find that four biochemical treatments (a serine protease, a Src kinase inhibitor, methyl-ß-..
- Hydrogen peroxide sensing and signaling
Elizabeth A Veal
Institute for Cell and Molecular Biosciences, Newcastle University, Framlington Place, Newcastle upon Tyne, Tyne and Wear, UK
Mol Cell 26:1-14. 2007
..contain antioxidant enzymes that limit oxidative stress by detoxifying reactive oxygen species, including hydrogen peroxide. However, in eukaryotes, hydrogen peroxide also has important roles as a signaling molecule in the ..
- A caspase-related protease regulates apoptosis in yeast
Institute for Physiological Chemistry, University of Tubingen, Hoppe Seyler Strasse 4, 72076 Tubingen, Germany
Mol Cell 9:911-7. 2002
..b>Hydrogen peroxide treatment induces apoptosis together with a caspase-like enzymatic activity in yeast...
- Complex III releases superoxide to both sides of the inner mitochondrial membrane
Florian L Muller
Department of Cellular Biology, University of Texas Health Science Center, San Antonio, TX 78284 7762, USA
J Biol Chem 279:49064-73. 2004
..In the present work, using measurements of hydrogen peroxide (Amplex red) as well as superoxide (modified Cypridina luciferin analog and aconitase), we demonstrate that ..
- Nitric oxide, stomatal closure, and abiotic stress
Centre for Research in Plant Science, Faculty of Health and Life Sciences, University of the West of England, Bristol BS16 1QY, UK
J Exp Bot 59:165-76. 2008
..These are two processes that both involve NO as a key signalling intermediate...
- Catabolite control protein A controls hydrogen peroxide production and cell death in Streptococcus sanguinis
Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
J Bacteriol 193:516-26. 2011
Streptococcus sanguinis is a commensal oral bacterium producing hydrogen peroxide (H₂O₂) that is dependent on pyruvate oxidase (Spx) activity...
- Oxidative stress, caloric restriction, and aging
R S Sohal
Department of Biological Sciences, Southern Methodist University, Dallas, TX 75275, USA
Science 273:59-63. 1996
..inversely correlate with the rates of mitochondrial generation of the superoxide anion radical (O2) and hydrogen peroxide. (iii) Restriction of caloric intake lowers steady-state levels of oxidative stress and damage, retards age-..
- Reversible inactivation of the tumor suppressor PTEN by H2O2
Seung Rock Lee
Laboratory of Cell Signaling, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 8015, USA
J Biol Chem 277:20336-42. 2002
- Oxidation of tetrahydrobiopterin leads to uncoupling of endothelial cell nitric oxide synthase in hypertension
Division of Cardiology, Emory University School of Medicine and Atlanta Veterans Administration Hospital, 1639 Pierce Drive, Atlanta, GA 30322, USA
J Clin Invest 111:1201-9. 2003
..is only minimally altered in vessels of mice with DOCA-salt hypertension but seems to be mediated by hydrogen peroxide released from uncoupled eNOS, since it is inhibited by catalase...
- Hydrogen peroxide: a metabolic by-product or a common mediator of ageing signals?
Marco Giorgio, Enrica Migliaccio and Pier Giuseppe Pelicci are at the Department of Experimental Oncology, IEO European Institute of Oncology, and IFOM FIRC Institute of Molecular Oncology, 20139 Milan, Italy
Nat Rev Mol Cell Biol 8:722-8. 2007
The reactive oxygen species that are generated by mitochondrial respiration, including hydrogen peroxide (H2O2), are potent inducers of oxidative damage and mediators of ageing...
- ROS, mitochondria and the regulation of autophagy
Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, 76100 Israel
Trends Cell Biol 17:422-7. 2007
..We then discuss the relationship between mitochondria and autophagosomes and propose that mitochondria have an essential role in autophagosome biogenesis...
- Reactive oxygen species: a breath of life or death?
John P Fruehauf
Department of Medicine, Chao Family Comprehensive Cancer Center, University of California, Irvine, Orange, California 92668, USA
Clin Cancer Res 13:789-94. 2007
..This review will cover recent work in ROS-mediated signaling in cancer cells and its potential as a target for developmental therapeutics...
- DNA microarray-mediated transcriptional profiling of the Escherichia coli response to hydrogen peroxide
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
J Bacteriol 183:4562-70. 2001
The genome-wide transcription profile of Escherichia coli cells treated with hydrogen peroxide was examined with a DNA microarray composed of 4,169 E. coli open reading frames...
- Hydrogen peroxide and nitric oxide as signalling molecules in plants
Steven J Neill
Centre for Research in Plant Science, Faculty of Applied Sciences, University of the West of England, Coldharbour Lane, Bristol BS16 1QY, UK
J Exp Bot 53:1237-47. 2002
It is now clear that hydrogen peroxide (H(2)O(2)) and nitric oxide (NO) function as signalling molecules in plants. A wide range of abiotic and biotic stresses results in H(2)O(2) generation, from a variety of sources...
- The E-loop is involved in hydrogen peroxide formation by the NADPH oxidase Nox4
Institut für Kardiovaskuläre Physiologie, Goethe Universitat, 60596 Frankfurt am Main, Germany
J Biol Chem 286:13304-13. 2011
In contrast to the NADPH oxidases Nox1 and Nox2, which generate superoxide (O(2)(·-)), Nox4 produces hydrogen peroxide (H(2)O(2))...
- Rapamycin sensitive mTOR activation mediates nerve growth factor (NGF) induced cell migration and pro-survival effects against hydrogen peroxide in retinal pigment epithelial cells
Guo Fan Cao
The Affiliated Eye Hospital of Nanjing Medical University, Nanjing 210029, China
Biochem Biophys Res Commun 414:499-505. 2011
..human retinal pigment epithelial (RPE) cells, we demonstrate here that NGF protects RPE cells against hydrogen peroxide (H(2)O(2))-induced cell apoptosis...
- Cancer cells metabolically "fertilize" the tumor microenvironment with hydrogen peroxide, driving the Warburg effect: implications for PET imaging of human tumors
Ubaldo E Martinez-Outschoorn
The Jefferson Stem Cell Biology and Regenerative Medicine Center, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, USA
Cell Cycle 10:2504-20. 2011
..Interestingly, using this approach, we directly show that cancer cells initially secrete hydrogen peroxide that then triggers oxidative stress in neighboring fibroblasts...
- Mitochondrial free radical generation, oxidative stress, and aging
Department of Molecular Pharmacology and Toxicology, School of Pharmacy University of Southern California, Los Angeles 90033, USA
Free Radic Biol Med 29:222-30. 2000
..chain with molecular oxygen directly generate the superoxide anion radical (O2*-), which dismutates to form hydrogen peroxide (H2O2), which can further react to form the hydroxyl radical (HO*)...
- Ginsenoside Rg1 protects against hydrogen peroxide-induced cell death in PC12 cells via inhibiting NF-κB activation
Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing, PR China
Neurochem Int 58:119-25. 2011
..However, its molecular mechanism against oxidative stress induced by exogenous hydrogen peroxide (H(2)O(2)) remained unclear...
- Hydrogen peroxide: a signaling messenger
James R Stone
Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
Antioxid Redox Signal 8:243-70. 2006
b>Hydrogen peroxide (H2O2) is a well-documented component of living cells. It plays important roles in host defense and oxidative biosynthetic reactions...
- Towards a unifying, systems biology understanding of large-scale cellular death and destruction caused by poorly liganded iron: Parkinson's, Huntington's, Alzheimer's, prions, bactericides, chemical toxicology and others as examples
Douglas B Kell
School of Chemistry and The Manchester Interdisciplinary Biocentre, The University of Manchester, Manchester M1 7DN, UK
Arch Toxicol 84:825-89. 2010
..A variety of systems biology approaches, that I summarise, can predict both the mechanisms involved in these cell death pathways and the optimal sites of action for nutritional or pharmacological interventions...
- Oxidative stress and nuclear factor-kappaB activation: a reassessment of the evidence in the light of recent discoveries
Department of Biochemistry, Trinity College, Dublin, Ireland
Biochem Pharmacol 59:13-23. 2000
..In particular, the activation of NFkappaB by hydrogen peroxide is cell-specific and distinct from physiological activators such as IL-1 and TNF, while inhibition by ..
- Peroxiredoxin evolution and the regulation of hydrogen peroxide signaling
Zachary A Wood
Department of Biochemistry and Biophysics, Oregon State University, Corvallis, OR 97333, USA
Science 300:650-3. 2003
Eukaryotic 2-Cys peroxiredoxins (2-Cys Prxs) not only act as antioxidants, but also appear to regulate hydrogen peroxide-mediated signal transduction...
- Generating disulfides enzymatically: reaction products and electron acceptors of the endoplasmic reticulum thiol oxidase Ero1p
Department of Structural Biology, The Weizmann Institute of Science, Rehovot 76100, Israel
Proc Natl Acad Sci U S A 103:299-304. 2006
..Under aerobic conditions, reduction of molecular oxygen by Ero1p yielded stoichiometric hydrogen peroxide. Remarkably, we found that reduced Ero1p can transfer electrons to a variety of small and macromolecular ..
- Could heat shock transcription factors function as hydrogen peroxide sensors in plants?
Department of Biochemistry and Molecular Biology, University of Nevada, Mail Stop 200, Reno, NV 89557, USA
Ann Bot 98:279-88. 2006
- Activated granulocytes and granulocyte-derived hydrogen peroxide are the underlying mechanism of suppression of t-cell function in advanced cancer patients
Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
Cancer Res 61:4756-60. 2001
..This action is abrogated by the addition of the hydrogen peroxide (H(2)O(2)) scavenger, catalase, implicating H(2)O(2) as the effector molecule...
- Superoxide dismutase in redox biology: the roles of superoxide and hydrogen peroxide
Garry R Buettner
Free Radical and Radiation Biology, The University of Iowa, Iowa City, 52242 1181, USA
Anticancer Agents Med Chem 11:341-6. 2011
..These observations indicated that SOD, superoxide, and hydrogen peroxide affected the basic biology of cells and tissues, not just via damaging oxidation reactions...
- Rutin inhibits hydrogen peroxide-induced apoptosis through regulating reactive oxygen species mediated mitochondrial dysfunction pathway in human umbilical vein endothelial cells
Institute for Nanobiomedical Technology and Membrane Biology, State Key Lab of Biotherapy of Human Diseases, Cancer Center, West China Hospital, West China Medical School, Sichuan University, Sichuan, PR China
Eur J Pharmacol 628:27-35. 2010
..protective effects of rutin against apoptosis of human umbilical vein endothelial cells (HUVECs) induced by hydrogen peroxide (H(2)O(2)) and the associated signaling pathways...
- Hydrogen peroxide production by Lactobacillus johnsonii NCC 533 and its role in anti-Salmonella activity
Raymond David Pridmore
Department of Nutrition and Health, Nestle Research Center, Vers chez les Blancs, Lausanne, Switzerland
FEMS Microbiol Lett 283:210-5. 2008
..identified three potential gene products that may catalyze the synthesis of the known antimicrobial factor hydrogen peroxide, H(2)O(2). In this work, we confirmed the ability of NCC 533 as well as eight different L...
- Association of oxidative stress with postmenopausal osteoporosis and the effects of hydrogen peroxide on osteoclast formation in human bone marrow cell cultures
Ki Hyun Baek
Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea
Calcif Tissue Int 87:226-35. 2010
..In addition, RANKL and M-CSF stimulation induced by oxidative stress may participate in osteoclastogenesis in human bone...
- Human umbilical cord blood-derived mesenchymal stem cells undergo cellular senescence in response to oxidative stress
Department of Biological Sciences, Seoul National University, Seoul, Korea
Stem Cells Dev 21:1877-86. 2012
..Therefore, the proliferation and fate of hUCB-MSCs can be controlled by exposure to oxidative stresses...
- A hydrogen peroxide-inactivated virus vaccine elicits humoral and cellular immunity and protects against lethal West Nile virus infection in aged mice
Amelia K Pinto
Department of Medicine, Washington University School of Medicine, St Louis, Missouri, USA
J Virol 87:1926-36. 2013
..Here, we describe the generation and preclinical efficacy of a hydrogen peroxide (H(2)O(2))-inactivated WNV Kunjin strain (WNV-KUNV) vaccine as a candidate for further development...
- A Mitogen-activated protein kinase kinase kinase mediates reactive oxygen species homeostasis in Arabidopsis
Department of Plant Molecular Biology, Max F Perutz Laboratories, University of Vienna, Dr Bohrgasse 9, A 1030 Vienna, Austria
J Biol Chem 281:38697-704. 2006
..Overall, our data defines MPK4 as downstream target of MEKK1 and show that MEKK1 functions in integrating ROS homeostasis with plant development and hormone signaling...
- Molecular evolution of hydrogen peroxide degrading enzymes
Division of Biochemistry, Department of Chemistry, Vienna Institute of Biotechnology at BOKU University of Natural Resources and Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
Arch Biochem Biophys 525:131-44. 2012
For efficient removal of intra- and/or extracellular hydrogen peroxide by dismutation to harmless dioxygen and water (2H(2)O(2) → O(2) + 2H(2)O), nature designed three metalloenzyme families that differ in oligomeric organization, ..
- Oxidative protein folding by an endoplasmic reticulum-localized peroxiredoxin
Kimmel Center for Biology and Medicine at the Skirball Institute, New York University School of Medicine, New York, NY 10016, USA
Mol Cell 40:787-97. 2010
..These observations implicate ER-localized PRDX4 in a previously unanticipated, parallel, ERO1-independent pathway that couples hydroperoxide production to oxidative protein folding in mammalian cells...
- In vivo mapping of hydrogen peroxide and oxidized glutathione reveals chemical and regional specificity of redox homeostasis
Simone C Albrecht
Division of Redox Regulation, DKFZ ZMBH Alliance, German Cancer Research Center DKFZ, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
Cell Metab 14:819-29. 2011
The glutathione redox couple (GSH/GSSG) and hydrogen peroxide (H(2)O(2)) are central to redox homeostasis and redox signaling, yet their distribution within an organism is difficult to measure...
- Hydrogen peroxide: a Jekyll and Hyde signalling molecule
D R Gough
Tumour Biology Laboratory, Biochemistry Department, Bioscience Research Institute, University College Cork, Cork, Ireland
Cell Death Dis 2:e213. 2011
..Endogenous ROS such as hydrogen peroxide (H₂O₂) have long been recognised as destructive molecules...
- Peroxiredoxin functions as a peroxidase and a regulator and sensor of local peroxides
Sue Goo Rhee
Division of Life and Pharmaceutical Sciences, Ewha Womans University, Seoul 120 750, Korea
J Biol Chem 287:4403-10. 2012
..The fact that the oxidation state of the active site cysteine of Prx can be transferred to other proteins that are less intrinsically susceptible to H(2)O(2) also allows Prx to function as an H(2)O(2) sensor...
- Oxidative stress activates a positive feedback between the gamma- and beta-secretase cleavages of the beta-amyloid precursor protein
Department of Experimental Medicine and Oncology, University of Torino, Torino, Italy
J Neurochem 104:683-95. 2008
..Our findings suggest a mechanism by which OS induces BACE1 transcription, thereby promoting production of pathological levels of amyloid beta in AD...
- Positive feedback regulation of maize NADPH oxidase by mitogen-activated protein kinase cascade in abscisic acid signalling
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People s Republic of China
J Exp Bot 60:3221-38. 2009
..The results suggest that there is a positive feedback loop involving NADPH oxidase, H(2)O(2), and ZmMPK5 in ABA signalling...
- JunD reduces tumor angiogenesis by protecting cells from oxidative stress
Unit of Gene Expression and Diseases, CNRS URA 1644, Pasteur Institute, 25 rue du Docteur Roux, 75724 Paris, Cedex 15, France
Cell 118:781-94. 2004
..Furthermore, we provide new insights into the regulation of PHD activity, allowing immediate reactive adaptation to changes in O2 or iron levels in the cell...
- Photo-irradiated titanium dioxide catalyzes site specific DNA damage via generation of hydrogen peroxide
Department of Radiation Chemistry, Life Science Research Center, Mie University, Edobashi 2 174, Tsu Mie 514 8507, Japan
Free Radic Res 38:439-47. 2004
..SOD and bathocuproine, a chelator of Cu(I), inhibited the DNA damage, suggesting the involvement of hydrogen peroxide, superoxide and Cu(I)...
- Cytosolic ascorbate peroxidase 1 is a central component of the reactive oxygen gene network of Arabidopsis
Department of Biochemistry and Molecular Biology, University of Nevada, Reno, Nevada 89557, USA
Plant Cell 17:268-81. 2005
..Our study points to a key role for the cytosol in protecting the chloroplast during light stress and provides evidence for cross-compartment protection of thylakoid and stromal/mitochondrial APXs by cytosolic APX1...
- Imaging dynamic redox changes in mammalian cells with green fluorescent protein indicators
Colette T Dooley
Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, California 92093 0647, USA
J Biol Chem 279:22284-93. 2004
..g. epidermal growth factor and lysophosphatidic acid...
- Identification of the NF-E2-related factor-2-dependent genes conferring protection against oxidative stress in primary cortical astrocytes using oligonucleotide microarray analysis
Jong Min Lee
School of Pharmacy, Molecular and Environmental Toxicology Center, University of Wisconsin, Madison, Wisconsin 53705, USA
J Biol Chem 278:12029-38. 2003
- OXI1 kinase is necessary for oxidative burst-mediated signalling in Arabidopsis
Maike C Rentel
Department of Plant Sciences, University of Oxford, Oxford OX1 3RB, UK
Nature 427:858-61. 2004
..Thus, OXI1 is an essential part of the signal transduction pathway linking oxidative burst signals to diverse downstream responses...
- An Arabidopsis glutathione peroxidase functions as both a redox transducer and a scavenger in abscisic acid and drought stress responses
Henan Key Laboratory of Plant Stress Biology, Department of Biology, Henan University, Kaifeng 475001, China
Plant Cell 18:2749-66. 2006
- Kallikrein-modified mesenchymal stem cell implantation provides enhanced protection against acute ischemic kidney injury by inhibiting apoptosis and inflammation
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
Hum Gene Ther 19:807-19. 2008
..These results indicate that implantation of kallikrein-modified MSCs in the kidney provides advanced benefits in protection against ischemia-induced kidney injury by suppression of apoptosis and inflammation...
- Activation of dual oxidases Duox1 and Duox2: differential regulation mediated by camp-dependent protein kinase and protein kinase C-dependent phosphorylation
Institut de Recherche Interdisciplinaire en Biologie Humaine et Moleculaire, Université Libre Bruxelles, Brussels, Belgium
J Biol Chem 284:6725-34. 2009
..Our data provide, for the first time, detailed insights into the mechanisms controlling the activation of Duox1-2 proteins and reveal additional phosphorylation-mediated regulation...
- Hydrogen peroxide signalling
Centre for Research in Plant Science, Faculty of Applied Sciences, University of the West of England UWE, Frenchay, Bristol, BS161QY, UK
Curr Opin Plant Biol 5:388-95. 2002
Recent biochemical and genetic studies confirm that hydrogen peroxide is a signalling molecule in plants that mediates responses to abiotic and biotic stresses...
- Redox signaling: hydrogen peroxide as intracellular messenger
S G Rhee
Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Exp Mol Med 31:53-9. 1999
..The goal of this report is to review our current knowledge of ROS-regulated signal transduction and propose future directions...
- The apoplastic oxidative burst in response to biotic stress in plants: a three-component system
G Paul Bolwell
Division of Biochemistry, School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 0EX, UK
J Exp Bot 53:1367-76. 2002
..Thus it is possible that Arabidopsis is another species with the potential to mount an apoplastic oxidative burst and these transformed plant lines may be useful to identify the peroxidase that is responsible...
- Hydrogen peroxide as second messenger in lymphocyte activation
Max Planck Institute für Immunobiologie, Universitat Freiburg, Biologie III, Abteilung Molekulare Immunologie, Stuebeweg 51, Freiburg 79108, Germany
Nat Immunol 3:1129-34. 2002
..Thus, by controlling the activity of the negative regulatory phosphatases inside the cell, H2O2 can set and influence critical thresholds for lymphocyte activation...
- Hydroethidine- and MitoSOX-derived red fluorescence is not a reliable indicator of intracellular superoxide formation: another inconvenient truth
Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
Free Radic Biol Med 48:983-1001. 2010
- Regeneration of peroxiredoxins by p53-regulated sestrins, homologs of bacterial AhpD
Andrei V Budanov
Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
Science 304:596-600. 2004
Acting as a signal, hydrogen peroxide circumvents antioxidant defense by overoxidizing peroxiredoxins (Prxs), the enzymes that metabolize peroxides...
- Increased oxidative stress in obesity and its impact on metabolic syndrome
Department of Medicine and Pathophysiology, Graduate School of Medicine, Department of Organismal Biosystems, Graduate School of Frontier Biosciences, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
J Clin Invest 114:1752-61. 2004
- Trehalose accumulation during cellular stress protects cells and cellular proteins from damage by oxygen radicals
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 276:24261-7. 2001
..Therefore, trehalose accumulation in stressed cells plays a major role in protecting cellular constituents from oxidative damage...
- Angiotensin II stimulation of NAD(P)H oxidase activity: upstream mediators
Puvi N Seshiah
Division of Cardiology, Emory University, Atlanta, GA 30322, USA
Circ Res 91:406-13. 2002
- Reactive oxygen species as a signal in glucose-stimulated insulin secretion
Endocrine Biology Program, The Hamner Institutes for Health Sciences, 6 Davis Dr, Research Triangle Park, NC 27709, USA
Diabetes 56:1783-91. 2007
..Taken together, these findings suggest that H2O2 derived from glucose metabolism is one of the metabolic signals for insulin secretion, whereas oxidative stress may disturb its signaling function...
- Mitochondrial DNA mutations, oxidative stress, and apoptosis in mammalian aging
G C Kujoth
Departments of Genetics and Medical Genetics, University of Wisconsin, Madison, WI 53706, USA
Science 309:481-4. 2005
..The levels of apoptotic markers were also found to increase during aging in normal mice. Thus, accumulation of mtDNA mutations that promote apoptosis may be a central mechanism driving mammalian aging...
- Blocking late sodium current reduces hydrogen peroxide-induced arrhythmogenic activity and contractile dysfunction
Division of Cardiovascular Medicine, University of Florida, 1600 SW Archer Rd, M 411, Gainesville, FL 32610 0277, USA
J Pharmacol Exp Ther 318:214-22. 2006
- Mitochondrial alpha-ketoglutarate dehydrogenase complex generates reactive oxygen species
Anatoly A Starkov
Department of Neurology and Neuroscience, Weill Medical College, Cornell University, New York, New York 10021, USA
J Neurosci 24:7779-88. 2004
..These mitochondria also produced significantly less H(2)O(2) than mitochondria isolated from their littermate wild-type mice. The data strongly indicate that KGDHC is a primary site of ROS production in normally functioning mitochondria...
- Mitogen-activated protein kinase is involved in abscisic acid-induced antioxidant defense and acts downstream of reactive oxygen species production in leaves of maize plants
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People s Republic of China
Plant Physiol 141:475-87. 2006
..ABA-induced H(2)O(2) production activates MAPK, which in turn induces the expression and the activities of antioxidant enzymes. The activation of MAPK also enhances the H(2)O(2) production, forming a positive feedback loop...
- Generation of reactive oxygen species in the reaction catalyzed by alpha-ketoglutarate dehydrogenase
Department of Medical Biochemistry, Semmelweis University, and Neurochemistry Group, Hungarian Academy of Sciences, Budapest H 1444, Hungary
J Neurosci 24:7771-8. 2004
..Thus alpha-KGDH is not only a target of ROS but could significantly contribute to generation of oxidative stress in the mitochondria...
- Effect of miR-23 on oxidant-induced injury in human retinal pigment epithelial cells
Departments of Ophthalmology and Visual Sciences, University of Texas Medical Branch, Galveston, Texas 77555, USA
Invest Ophthalmol Vis Sci 52:6308-14. 2011
..The purpose of this study was to investigate the role of miR-23a in modulating RPE cell survival and gene expression in response to oxidative damage...
- Genome-wide analysis of hydrogen peroxide-regulated gene expression in Arabidopsis reveals a high light-induced transcriptional cluster involved in anthocyanin biosynthesis
Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, Belgium
Plant Physiol 139:806-21. 2005
In plants, reactive oxygen species and, more particularly, hydrogen peroxide (H(2)O(2)) play a dual role as toxic by-products of normal cell metabolism and as regulatory molecules in stress perception and signal transduction...
- A rice calcium-dependent protein kinase OsCPK12 oppositely modulates salt-stress tolerance and blast disease resistance
National Institute of Agrobiological Sciences, 2 1 2 Kannondai, Tsukuba, Ibaraki 305 8602, Japan
Plant J 69:26-36. 2012
..The accumulation of hydrogen peroxide (H(2) O(2) ) in the leaves was less in OsCPK12-OX plants than in wild-type (WT) plants...
- Hydrogen peroxide fuels aging, inflammation, cancer metabolism and metastasis: the seed and soil also needs "fertilizer"
Michael P Lisanti
The Jefferson Stem Cell Biology and Regenerative Medicine Center, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, USA
Cell Cycle 10:2440-9. 2011
..In this regard, a series of recent studies have elegantly shown that the production of hydrogen peroxide, by both cancer cells and cancer-associated fibroblasts, may provide the necessary "fertilizer," by driving ..
- Hydrogen peroxide-dependent DNA release and transfer of antibiotic resistance genes in Streptococcus gordonii
Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
J Bacteriol 193:6912-22. 2011
- Caloric restriction or catalase inactivation extends yeast chronological lifespan by inducing H2O2 and superoxide dismutase activity
Instituto de Investigação em Ciências da Vida e Saúde, Escola de Ciências da Saúde, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
Proc Natl Acad Sci U S A 107:15123-8. 2010
..of catalases extends chronological lifespan by inducing elevated levels of the reactive oxygen species hydrogen peroxide, which activate superoxide dismutases that inhibit the accumulation of superoxide anions...
- Characterization of hydrogen peroxide-induced DNA release by Streptococcus sanguinis and Streptococcus gordonii
Department of Diagnostic and Biological Sciences, School of Dentistry, University of Minnesota, Minneapolis, Minnesota 55455, USA
J Bacteriol 191:6281-91. 2009
..and Streptococcus gordonii release DNA in a process induced by pyruvate oxidase-dependent production of hydrogen peroxide (H(2)O(2)). Surprisingly, S. sanguinis and S...
- The impact of oxidative stress on Arabidopsis mitochondria
L J Sweetlove
Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK
Plant J 32:891-904. 2002
- The multifaceted capacity of Dps proteins to combat bacterial stress conditions: Detoxification of iron and hydrogen peroxide and DNA binding
Department of Biochemical Sciences A Rossi Fanelli, Sapienza University of Rome, Rome, Italy
Biochim Biophys Acta 1800:798-805. 2010
..The widely expressed Dps proteins, so named after the DNA-binding properties of the first characterized member of the family in Escherichia coli, are considered major players in the bacterial response to stress...