ethylnitrosourea

Summary

Summary: A nitrosourea compound with alkylating, carcinogenic, and mutagenic properties.

Top Publications

  1. pmc Massively parallel sequencing of the mouse exome to accurately identify rare, induced mutations: an immediate source for thousands of new mouse models
    T D Andrews
    Immunogenomics Laboratory, Australian National University, GPO Box 334, Canberra City, Australian Capital Territory, 2601, Australia
    Open Biol 2:120061. 2012
  2. ncbi ENU mutagenesis, a way forward to understand gene function
    Abraham Acevedo-Arozena
    MRC Mammalian Genetics Unit, Harwell, Oxfordshire, OX11 0RD, United Kingdom
    Annu Rev Genomics Hum Genet 9:49-69. 2008
  3. pmc Mutagenesis and mapping of a mouse gene, Clock, essential for circadian behavior
    M H Vitaterna
    Department of Neurobiology and Physiology, Northwestern University, Evanston, IL 60208
    Science 264:719-25. 1994
  4. ncbi Mouse mutagenesis with the chemical supermutagen ENU
    Frank J Probst
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA
    Methods Enzymol 477:297-312. 2010
  5. pmc The ataxia3 mutation in the N-terminal cytoplasmic domain of sodium channel Na(v)1.6 disrupts intracellular trafficking
    Lisa M Sharkey
    Department of Human Genetics, University of Michigan, Ann Arbor, Michigan 48109 5618, USA
    J Neurosci 29:2733-41. 2009
  6. ncbi ENU-based gene-driven mutagenesis in the mouse: a next-generation gene-targeting system
    Yoichi Gondo
    Mutagenesis and Genomics Team, RIKEN BioResource Center, Ibaraki, Japan
    Exp Anim 59:537-48. 2010
  7. ncbi Genome-wide ENU mutagenesis to reveal immune regulators
    K A Nelms
    Australian Cancer Research Foundation Genetics Laboratory, Medical Genome Centre, John Curtin School of Medical Research, Canberra ACT 2601, Australia
    Immunity 15:409-18. 2001
  8. pmc A novel ENU-mutation in ankyrin-1 disrupts malaria parasite maturation in red blood cells of mice
    Andreas Greth
    The Menzies Research Institute of Tasmania, University of Tasmania, Hobart, Australia
    PLoS ONE 7:e38999. 2012
  9. pmc ENU-induced phenovariance in mice: inferences from 587 mutations
    Carrie N Arnold
    Department of Genetics, The Scripps Research Institute, La Jolla, CA 92037, USA
    BMC Res Notes 5:577. 2012
  10. ncbi Genome-wide, large-scale production of mutant mice by ENU mutagenesis
    M H Hrabe de Angelis
    Institute of Experimental Genetics, GSF Research Center for Environment and Health, Neuherberg, Germany
    Nat Genet 25:444-7. 2000

Detail Information

Publications248 found, 100 shown here

  1. pmc Massively parallel sequencing of the mouse exome to accurately identify rare, induced mutations: an immediate source for thousands of new mouse models
    T D Andrews
    Immunogenomics Laboratory, Australian National University, GPO Box 334, Canberra City, Australian Capital Territory, 2601, Australia
    Open Biol 2:120061. 2012
    ..This approach transforms genetic screens in mice, establishes a general strategy for analysing rare DNA variants and opens up a large new source for experimental models of human disease...
  2. ncbi ENU mutagenesis, a way forward to understand gene function
    Abraham Acevedo-Arozena
    MRC Mammalian Genetics Unit, Harwell, Oxfordshire, OX11 0RD, United Kingdom
    Annu Rev Genomics Hum Genet 9:49-69. 2008
    ..N-ethyl-N-nitrosourea (ENU) mutagenesis has been widely used for both approaches to generate mouse mutants. Here we review progress in ENU mutagenesis screening, with an emphasis on creating mouse models for human disorders...
  3. pmc Mutagenesis and mapping of a mouse gene, Clock, essential for circadian behavior
    M H Vitaterna
    Department of Neurobiology and Physiology, Northwestern University, Evanston, IL 60208
    Science 264:719-25. 1994
    ..The power of ENU mutagenesis combined with the ability to clone murine genes by map position provides a generally applicable approach to study complex behavior in mammals...
  4. ncbi Mouse mutagenesis with the chemical supermutagen ENU
    Frank J Probst
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA
    Methods Enzymol 477:297-312. 2010
    ....
  5. pmc The ataxia3 mutation in the N-terminal cytoplasmic domain of sodium channel Na(v)1.6 disrupts intracellular trafficking
    Lisa M Sharkey
    Department of Human Genetics, University of Michigan, Ann Arbor, Michigan 48109 5618, USA
    J Neurosci 29:2733-41. 2009
    ..The data demonstrate that the cytoplasmic N-terminal domain of the sodium channel is required for anterograde transport from the Golgi complex to the plasma membrane...
  6. ncbi ENU-based gene-driven mutagenesis in the mouse: a next-generation gene-targeting system
    Yoichi Gondo
    Mutagenesis and Genomics Team, RIKEN BioResource Center, Ibaraki, Japan
    Exp Anim 59:537-48. 2010
    ..In the near future, next-generation re-sequencing systems should drastically enhance the utility of the ENU-based gene-driven mutagenesis not only for the mouse but also for other species...
  7. ncbi Genome-wide ENU mutagenesis to reveal immune regulators
    K A Nelms
    Australian Cancer Research Foundation Genetics Laboratory, Medical Genome Centre, John Curtin School of Medical Research, Canberra ACT 2601, Australia
    Immunity 15:409-18. 2001
    ..Our recent experience indicates that genome-wide chemical mutagenesis in the mouse is an extremely powerful methodology for the identification of genes required for complex immunological processes...
  8. pmc A novel ENU-mutation in ankyrin-1 disrupts malaria parasite maturation in red blood cells of mice
    Andreas Greth
    The Menzies Research Institute of Tasmania, University of Tasmania, Hobart, Australia
    PLoS ONE 7:e38999. 2012
    ..05). We conclude that increased malaria resistance due to ankyrin-1 deficiency is caused by the intraerythrocytic death of P. chabaudi parasites...
  9. pmc ENU-induced phenovariance in mice: inferences from 587 mutations
    Carrie N Arnold
    Department of Genetics, The Scripps Research Institute, La Jolla, CA 92037, USA
    BMC Res Notes 5:577. 2012
    ....
  10. ncbi Genome-wide, large-scale production of mutant mice by ENU mutagenesis
    M H Hrabe de Angelis
    Institute of Experimental Genetics, GSF Research Center for Environment and Health, Neuherberg, Germany
    Nat Genet 25:444-7. 2000
    ..Our mutant lines are freely accessible to non-commercial users (for information, see http://www.gsf.de/ieg/groups/enu-mouse.html)...
  11. pmc Analysis of an ethylnitrosourea-generated mouse mutation defines a cell intrinsic role of nuclear factor kappaB2 in regulating circulating B cell numbers
    Lisa A Miosge
    Australian Cancer Research Foundation Genetics Laboratory and Medical Genome Centre, John Curtin School of Medical Research, Australian National University, Canberra ACT 2601, Australia
    J Exp Med 196:1113-9. 2002
    ..These findings establish the feasibility of analyzing immune regulation by genome wide mutant screens and demonstrates an intrinsic requirement for NF-kappaB2 in regulating circulating follicular B cell numbers...
  12. pmc Novel roles for erythroid Ankyrin-1 revealed through an ENU-induced null mouse mutant
    Gerhard Rank
    Rotary Bone Marrow Research Laboratory, Melbourne Health Research Directorate, Parkville, Australia
    Blood 113:3352-62. 2009
    ..These findings provide novel insights into the role of Ank-1, and define an ideal model for the study of HS and malarial resistance...
  13. pmc Lampe1: an ENU-germline mutation causing spontaneous hepatosteatosis identified through targeted exon-enrichment and next-generation sequencing
    Rachel Sheridan
    Department of Pathology and Laboratory Medicine, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio, United States of America
    PLoS ONE 6:e21979. 2011
    ..The current approach provides a highly efficient and affordable method to identify causative mutations induced by ENU mutagenesis and animal models relevant to human pathology...
  14. ncbi ENU mutagenesis in the mouse: application to human genetic disease
    Patrick M Nolan
    Neurobehavioural Genetics Group, Medical Research Council Mammalian Genetics Unit, Harwell, Didcot, UK
    Brief Funct Genomic Proteomic 1:278-89. 2002
    ..In this paper, progress with existing phenotypic screening programmes is discussed and opportunities for the development of new mouse disease models are presented...
  15. ncbi Aberrant nestin expression during ethylnitrosourea-(ENU)-induced neurocarcinogenesis
    Taichang Jang
    Department of Surgery Neurosurgery, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Neurobiol Dis 15:544-52. 2004
    ..that nestin would be a sensitive marker of early neoplasia after transplacental exposure of rats to ethylnitrosourea (ENU)...
  16. ncbi Unraveling innate immunity using large scale N-ethyl-N-nitrosourea mutagenesis
    K Hoebe
    Department of Immunology IMM 31, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Tissue Antigens 65:395-401. 2005
    ..In this review, we will discuss the potential of the forward genetic approach and ENU mutagenesis to identify new genes and new functions of known genes related to innate immunity...
  17. ncbi Phenotypic characterization of a new Grin1 mutant mouse generated by ENU mutagenesis
    Tamio Furuse
    Technology and Development Team for Mouse Phenotype Analysis Japan Mouse Clinic, RIKEN BioResource Center, 3 1 1 Koyadai, Tsukuba, Ibaraki 305 0074, Japan
    Eur J Neurosci 31:1281-91. 2010
    ..The results of immunohistochemical and biochemical analyses suggested that impaired interaction between the glutamatergic pathway and dopaminergic pathway may underlie the behavioral phenotypes of the Grin1(Rgsc174) mutant...
  18. ncbi A hearing and vestibular phenotyping pipeline to identify mouse mutants with hearing impairment
    Rachel E Hardisty-Hughes
    MRC Harwell, Mammalian Genetics Unit, Harwell Science and Innovation Campus, Harwell, UK
    Nat Protoc 5:177-90. 2010
    ..5 h per mouse. These protocols have been applied for the identification of mouse models of human deafness and are a key component for investigating the genes and genetic pathways involved in hereditary deafness...
  19. pmc An ENU-mutagenesis screen in the mouse: identification of novel developmental gene functions
    Carolien Wansleeben
    Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Centre Utrecht, Utrecht, The Netherlands
    PLoS ONE 6:e19357. 2011
    ..Here we describe a phenotype-based screen for recessive mutations affecting embryonic development...
  20. ncbi Enamelin (Enam) is essential for amelogenesis: ENU-induced mouse mutants as models for different clinical subtypes of human amelogenesis imperfecta (AI)
    Hiroshi Masuya
    Mouse Functional Genomics Research Group, Tsukuba, Ibaraki, Japan
    Hum Mol Genet 14:575-83. 2005
    ..Thus, we report here that the Enam gene is essential for amelogenesis, and that mice with different point mutations at Enam may provide good animal models to study the different clinical subtypes of AI...
  21. pmc Mutational pattern and frequency of induced nucleotide changes in mouse ENU mutagenesis
    K Ryo Takahasi
    Population and Quantitative Genomics Team, RIKEN Genomic Sciences Center, Yokohama, Japan
    BMC Mol Biol 8:52. 2007
    ....
  22. pmc Melody, an ENU mutation in Caspase 3, alters the catalytic cysteine residue and causes sensorineural hearing loss in mice
    Andrew Parker
    MRC Mammalian Genetics Unit, MRC Harwell, Harwell Science and Innovation Campus, Oxfordshire, OX11 0RD, UK
    Mamm Genome 21:565-76. 2010
    ..The melody line provides a new model for studying the role of Caspase 3 in deafness and a number of other pathways and systems...
  23. pmc ENU mutagenesis reveals a novel phenotype of reduced limb strength in mice lacking fibrillin 2
    Gaynor Miller
    Mammalian Genetics of Disease Unit, School of Medicine, University of Sheffield, Sheffield, United Kingdom
    PLoS ONE 5:e9137. 2010
    ..In the case of Fbn2, four different mutant alleles have been described in the mouse and in all cases syndactyly was reported as the defining phenotypic feature of homozygotes...
  24. pmc Cytopenia induction by 5-fluorouracil identifies thrombopoietic mutants in sensitized ENU mutagenesis screens
    Nicole M Anderson
    Institute of Medical Science, Faculty of Medicine, University of Toronto, Toronto, Ont, Canada
    Exp Hematol 40:48-60. 2012
    ....
  25. ncbi Spectrum of ENU-induced mutations in phenotype-driven and gene-driven screens in the mouse
    Ivana Barbaric
    Department of Biomedical Science, University of Sheffield, Sheffield, United Kingdom
    Environ Mol Mutagen 48:124-42. 2007
    ..Knowledge of the spectrum of mutations that ENU elicits will assist in positional cloning of ENU-induced mutations by allowing prioritization of candidate genes based on some of their inherent features...
  26. pmc A novel ENU-generated truncation mutation lacking the spectrin-binding and C-terminal regulatory domains of Ank1 models severe hemolytic hereditary spherocytosis
    Michael R Hughes
    The Biomedical Research Centre, University of British Columbia, Vancouver, BC, Canada
    Exp Hematol 39:305-20, 320.e1-2. 2011
    ..We investigated a novel mouse model in which the heterozygotes present with the diagnostic hallmarks of mild HS and surviving homozygotes phenocopy severe hemolytic HS...
  27. ncbi A novel dominant-negative mutation in Gdf5 generated by ENU mutagenesis impairs joint formation and causes osteoarthritis in mice
    Hiroshi Masuya
    Mouse Functional Genomics Research Group, RIKEN GSC, Tsukuba, Ibaraki, Japan
    Hum Mol Genet 16:2366-75. 2007
    ..This study further highlights a critical role of GDF5 in joint formation and the development of OA, and this mouse should serve as a good model for OA...
  28. pmc Unlocking the bottleneck in forward genetics using whole-genome sequencing and identity by descent to isolate causative mutations
    Katherine R Bull
    Nuffield Department of Medicine and Wellcome Trust Centre for Human Genetics, Oxford University, Oxford, United Kingdom
    PLoS Genet 9:e1003219. 2013
    ..The introduction of this approach will accelerate the discovery of causal variants, permit broader and more informative lethal screens to be used, reduce animal costs, and herald a new era for ENU mutagenesis...
  29. ncbi N-ethyl-N-nitrosourea (ENU) increased brain mutations in prenatal and neonatal mice but not in the adults
    William Slikker
    College of Letters and Science, University of California, Los Angeles 90024, USA
    Toxicol Sci 81:112-20. 2004
    ..These results demonstrate a differential mutagenic effect of ENU on the mouse brain depending on the stages of development and suggest an enhanced susceptibility of brain cancer hazard for perinatal exposure to genotoxicants...
  30. pmc ENU mutagenesis identifies mice with morbid obesity and severe hyperinsulinemia caused by a novel mutation in leptin
    Chen Jee Hong
    Institute of Brain Science, School of Medicine, National Yang Ming University, Taipei, Taiwan
    PLoS ONE 5:e15333. 2010
    ..Obesity is a multifactorial disease that arises from complex interactions between genetic predisposition and environmental factors. Leptin is central to the regulation of energy metabolism and control of body weight in mammals...
  31. ncbi A genomewide screening of N-ethyl-N-nitrosourea-mutagenized mice for musculoskeletal phenotypes
    A K Srivastava
    Musculoskeletal Disease Center, Jerry L Pettis Veterans Medical Center, and Department of Medicine, Loma Linda University, Loma Linda, CA 92357, USA
    Bone 33:179-91. 2003
    ..So far, this ENU mutagenesis program has identified four outliers that can undergo positional cloning...
  32. pmc Comprehensive behavioral analysis of ENU-induced Disc1-Q31L and -L100P mutant mice
    Hirotaka Shoji
    Division of Systems Medical Science, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi, Japan
    BMC Res Notes 5:108. 2012
    ....
  33. pmc A focused and efficient genetic screening strategy in the mouse: identification of mutations that disrupt cortical development
    Konstantinos Zarbalis
    Department of Neurology and the Ernest Gallo Clinic and Research Center, University of California at San Francisco, Emeryville, USA
    PLoS Biol 2:E219. 2004
    ..Our results also highlight the utility of focused screens in the mouse, in addition to the large-scale and broadly targeted screens that are being carried out at mutagenesis centers...
  34. pmc Mutation in archain 1, a subunit of COPI coatomer complex, causes diluted coat color and Purkinje cell degeneration
    Xinjie Xu
    Department of Medical Genetics, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS Genet 6:e1000956. 2010
    ..Our study documents for the first time the physiological consequences of the impairment of the ARCN1 function in the whole animal and demonstrates a direct association between ARCN1 and neurodegeneration...
  35. pmc High resolution mapping and positional cloning of ENU-induced mutations in the Rw region of mouse chromosome 5
    Yung Hao Ching
    Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA
    BMC Genet 11:106. 2010
    ..Previously, a large scale ENU mutagenesis screen was conducted to query the functional content of a ~50 Mb region of the mouse genome on proximal Chr 5. The majority of phenotypic mutants recovered were embryonic lethals...
  36. ncbi ENU mutagenesis as a tool for understanding lung development and disease
    Laura Yates
    MRC Harwell, Harwell, Oxfordshire OX11 0RD, UK
    Biochem Soc Trans 37:838-42. 2009
    ..Functional analyses of these alleles are currently under way...
  37. ncbi A systematic, genome-wide, phenotype-driven mutagenesis programme for gene function studies in the mouse
    P M Nolan
    MRC Mammalian Genetics Unit and Mouse Genome Centre, Harwell, UK
    Nat Genet 25:440-3. 2000
    ..Phenotype-driven approaches using the chemical mutagen ethylnitrosourea (ENU) represent a potentially efficient route for the generation of large numbers of mutant mice that can be ..
  38. pmc An ENU-induced mutation of miR-96 associated with progressive hearing loss in mice
    Morag A Lewis
    Wellcome Trust Sanger Institute, Hinxton, UK
    Nat Genet 41:614-8. 2009
    ..This is the first microRNA found associated with deafness, and diminuendo represents a model for understanding and potentially moderating progressive hair cell degeneration in hearing loss more generally...
  39. ncbi ENU mutagenesis in mice
    Philippe Georgel
    Laboratoire d Immunogénétique Moléculaire Humaine, Centre de Recherche en Immunologie et Hématologie, Faculte de Medecine, Strasbourg Cedex, France
    Methods Mol Biol 415:1-16. 2008
    ..In this chapter, we will provide a practical guideline for performing germline mutagenesis in mice. In particular, we will focus on the application of this technology to identify genes that are essential to innate immune defense...
  40. ncbi Implementation of the modified-SHIRPA protocol for screening of dominant phenotypes in a large-scale ENU mutagenesis program
    Hiroshi Masuya
    Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3 1 1 Kouyadai, Tsukubai, Ibaraki, Japan
    Mamm Genome 16:829-37. 2005
    ..They could hold great potential for the unraveling of the molecular mechanisms of certain phenotypes...
  41. ncbi An ENU-induced mutation in the Ankrd11 gene results in an osteopenia-like phenotype in the mouse mutant Yoda
    Ivana Barbaric
    MRC Mammalian Genetics Unit, Harwell
    Physiol Genomics 32:311-21. 2008
    ..This gene has not been previously associated with bone metabolism and, thus, identifies a novel genetic regulator of bone homeostasis...
  42. ncbi A series of ENU-induced single-base substitutions in a long-range cis-element altering Sonic hedgehog expression in the developing mouse limb bud
    Hiroshi Masuya
    Mouse Functional Genomics Research Group, RIKEN GSC 3 1 1 Kouyadai, Tsukuba, Ibaraki 305 0074, Japan
    Genomics 89:207-14. 2007
    ..Thus, this study shows that gene-driven screening for ENU-induced mutations is an effective approach for exploring the function of conserved, noncoding sequences and potential cis-regulatory elements...
  43. pmc Exome sequencing identifies a missense mutation in Isl1 associated with low penetrance otitis media in dearisch mice
    Jennifer M Hilton
    Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK
    Genome Biol 12:R90. 2011
    ..Genetic studies suggest an inherited component, but few of the genes that contribute to this condition are known. Mouse mutants have contributed significantly to the identification of genes predisposing to otitis media..
  44. ncbi Efficient and fast targeted production of murine models based on ENU mutagenesis
    M Augustin
    Ingenium Pharmaceuticals AG, Fraunhoferstr 13, 82152 Martinsried, Germany
    Mamm Genome 16:405-13. 2005
    ..We conclude that ENU-based targeted mutagenesis is a powerful tool for the fast and high-throughput production of murine gene-specific models for biomedical research...
  45. ncbi Genetic mapping and ENU mutagenesis
    David R Beier
    Genetics Division, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Genetica 122:65-9. 2004
    ..Determination of the map position of a mutation early in its characterization can be extremely useful. We describe how the use of interval haplotype analysis can facilitate this with even small numbers of affected progeny...
  46. ncbi Efficient generation and mapping of recessive developmental mutations using ENU mutagenesis
    Bruce J Herron
    Genetics Division, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Genet 30:185-9. 2002
    ..Using a hierarchical approach, it is possible to maximize the efficiency of this analysis so that it can be carried out easily with modest infrastructure and resources...
  47. ncbi Reduced corneal thickness and enlarged anterior chamber in a novel ColVIIIa2G257D mutant mouse
    Oliver Puk
    Institute of Developmental Genetics, Helmholtz Center Munich, German Research Center for Environmental Health, Neuherberg, Germany
    Invest Ophthalmol Vis Sci 50:5653-61. 2009
    ..The purpose of this study was the morphologic and genetic characterization of the novel eye size mutant Aca23 in the mouse...
  48. ncbi Paternal exposure to ethylnitrosourea results in transgenerational genomic instability in mice
    Yuri E Dubrova
    Department of Genetics, University of Leicester, Leicester, United Kingdom
    Environ Mol Mutagen 49:308-11. 2008
    ..Here we have analyzed the effects of paternal treatment by ethylnitrosourea, an alkylating agent which is known to form specific types of DNA adducts, on the transgenerational effects ..
  49. pmc Diabetes insipidus in mice with a mutation in aquaporin-2
    David J Lloyd
    Genomics Institute of the Novartis Research Foundation, La Jolla, California, USA
    PLoS Genet 1:e20. 2005
    ..Through forward genetic screening of ethylnitrosourea-mutagenized mice, we report the identification and characterization of a mouse model of NDI, with an F204V ..
  50. pmc Identification of a rat model for usher syndrome type 1B by N-ethyl-N-nitrosourea mutagenesis-driven forward genetics
    Bart M G Smits
    Hubrecht Laboratory, Centre for Biomedical Genetics, 3584 CT Utrecht, The Netherlands
    Genetics 170:1887-96. 2005
    ..In addition, we demonstrate proof of principle for the generation and cloning of human disease models in rat using ENU mutagenesis, providing good perspectives for systematic phenotypic screens in the rat...
  51. ncbi Apoptotic cell death and cell proliferative activity in the rat fetal central nervous system from dams administered with ethylnitrosourea (ENU)
    K Katayama
    Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan
    Histol Histopathol 16:79-85. 2001
    b>Ethylnitrosourea (ENU), a well known DNA alkylating agent, induces anomalies in the central nervous system (CNS), craniofacial tissues and male reproductive organs, and the enhancement of apoptosis is found in these tissues immediately ..
  52. pmc An ENU-induced mutation in mouse glycyl-tRNA synthetase (GARS) causes peripheral sensory and motor phenotypes creating a model of Charcot-Marie-Tooth type 2D peripheral neuropathy
    Francesca Achilli
    Department of Neurodegenerative Disease, Institute of Neurology, University College London, London WC1N 3BG, UK
    Dis Model Mech 2:359-73. 2009
    ....
  53. ncbi Screening for novel ENU-induced rhythm, entrainment and activity mutants
    Y Bacon
    MRC Mammalian Genetics Unit, Harwell, UK
    Genes Brain Behav 3:196-205. 2004
    ..Mutant loci do not correspond with those previously associated with wheel-running behaviour. This result confirms that phenotype-driven approaches such as this should continue to provide material for mammalian gene function studies...
  54. pmc Phenotypic and genetic analysis of the cerebellar mutant tmgc26, a new ENU-induced ROR-alpha allele
    Douglas J Swanson
    Department of Medical Genetics, Centre for Molecular Medicine and Therapeutics, University of British Columbia, Vancouver, BC, Canada
    Eur J Neurosci 32:707-16. 2010
    ....
  55. ncbi Ethylnitrosourea induces neural progenitor cell apoptosis after S-phase accumulation in a p53-dependent manner
    Kei Ichi Katayama
    Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    Neurobiol Dis 18:218-25. 2005
    ..In this study, immediately after the administration of ethylnitrosourea (ENU), an alkylating agent, an accumulation of neural progenitor cells in the S phase was observed...
  56. ncbi Effect of the spin-labelled 1-ethyl-1-nitrosourea on CCNU-induced oxidative liver injury
    V Gadjeva
    Department of Chemistry and Biochemistry, Faculty of Medicine, Trakia University, Stara Zagora, Bulgaria
    Pharmazie 62:608-13. 2007
    ..This protective effect is through the scavenging of *O2- and by an increased production of *NO. Thus, a potential for developing new combination chemotherapy in cancer is seen...
  57. pmc A behavioral screen for isolating zebrafish mutants with visual system defects
    S E Brockerhoff
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 92:10545-9. 1995
    ..Histologically, the retinas of noa and poa larvae appeared normal. We conclude that noa larvae have a functional defect in the outer retina, whereas the outer retina of poa larvae is likely to be normal...
  58. pmc N-ethyl-N-nitrosourea-induced mutation in ubiquitin-specific peptidase 18 causes hyperactivation of IFN-αß signaling and suppresses STAT4-induced IFN-γ production, resulting in increased susceptibility to Salmonella typhimurium
    Etienne Richer
    Department of Human Genetics, McGill University, Montreal, Quebec, Canada
    J Immunol 185:3593-601. 2010
    ..The reduced IFN-gamma levels, along with the increased inflammation, rationalize the S. Typhimurium susceptibility in terms of increased bacterial load in target organs and cytokine-induced septic shock and death...
  59. pmc Rapid identification of a disease allele in mouse through whole genome sequencing and bulk segregation analysis
    Carrie N Arnold
    Department of Genetics, Institute for Childhood and Neglected Disease, The Scripps Research Institute, La Jolla, California 92037, USA
    Genetics 187:633-41. 2011
    ..Our study demonstrates the speed and utility of whole genome sequencing coupled with low resolution meiotic mapping as a means of identifying causative mutations induced by ENU...
  60. ncbi Identification of a novel point mutation of mouse Atp2b2 induced by N-ethyl-N-nitrosourea mutagenesis
    Lin Xu
    Department of Otolaryngology and Head and Neck Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China
    Exp Anim 60:71-8. 2011
    ..These results for the Elfin mutant confirm the role of ATP2B2 in balance, hearing and formation of otoconia and suggest it may serve as a new model of human hereditary hearing loss...
  61. pmc Catweasel mice: a novel role for Six1 in sensory patch development and a model for branchio-oto-renal syndrome
    Erika A Bosman
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton CB10 1SA, UK
    Dev Biol 328:285-96. 2009
    ..In addition Six1 has a pivotal role in early sensory patch development and may act in the same genetic pathway as Jag1...
  62. pmc An allelic series of mutations in Smad2 and Smad4 identified in a genotype-based screen of N-ethyl-N- nitrosourea-mutagenized mouse embryonic stem cells
    Jay L Vivian
    Department of Genetics, University of North Carolina, Chapel Hill 27599, USA
    Proc Natl Acad Sci U S A 99:15542-7. 2002
    ..This methodology can conceptually be applied for the generation of an allelic series of subtle mutations at any locus of interest in the mouse...
  63. ncbi ENU-mutagenesis: insight into immune function and pathology
    Matthew C Cook
    Frank Fenner Building, Australian National University, Canberra ACT 0200, Australia
    Curr Opin Immunol 18:627-33. 2006
    ..More than 20 of the phenotypes that exhibit a simple (Mendelian) pattern of inheritance have been mapped. Novel alleles have revealed new pathways of host defense, allergy and autoimmunity...
  64. ncbi Generation of ENU-induced mouse mutants with hypocholesterolemia: novel tools for dissecting plasma lipoprotein homeostasis
    Bernhard Aigner
    Institute of Molecular Animal Breeding and Biotechnology, Ludwig Maximilians University Munich, Hackerstr 27, 85764, Oberschleissheim, Germany
    Lipids 42:731-7. 2007
    ..Such findings may be subsequently used for humans and other species...
  65. ncbi Neurogenic tumors in rats induced by ethylnitrosourea
    Bernard C Zook
    Department of Pathology, The George Washington University Medical Center, Washington, DC 20037, USA
    Exp Toxicol Pathol 57:7-14. 2005
    b>Ethylnitrosourea (ENU) was injected intravenously into Sprague-Dawley rats on day 15 of gestation at doses of 0, 2.50, 6.25 and 10.00 mg/kg...
  66. pmc Massively parallel sequencing identifies the gene Megf8 with ENU-induced mutation causing heterotaxy
    Zhen Zhang
    Laboratory of Developmental Biology, National Heart Lung and Blood Institute, Bethesda, MD 20892 1583, USA
    Proc Natl Acad Sci U S A 106:3219-24. 2009
    ..Overall, through the recovery of an ENU-induced mutation, we uncovered Megf8 as an essential regulator of left-right patterning...
  67. ncbi Sequence-based analysis of mutagenized mice
    D R Beier
    Genetics Division, Brigham and Women s Hospital, 20 Shattuck Street, Boston, Massachusetts 02115, USA
    Mamm Genome 11:594-7. 2000
    Treating mice with ethylnitrosourea (ENU) is an efficient means for mutagenizing spermatogonial cells, and this treatment has been proven effective in a variety of screens for both dominant and recessive mutations...
  68. ncbi Generation of N-ethyl-N-nitrosourea-induced mouse mutants with deviations in hematological parameters
    Bernhard Aigner
    Department of Veterinary Sciences, Chair for Molecular Animal Breeding and Biotechnology, and Laboratory for Functional Genome Analysis LAFUGA, Gene Center, LMU Munich, Moorversuchsgut, Hackerstr 27, 85764, Oberschleiβheim, Munich, Germany
    Mamm Genome 22:495-505. 2011
    ..Thus, novel phenotype-driven mouse models were established to analyze the genetic components of hematological disorders...
  69. ncbi Behavioral and neuromorphological characterization of a novel Tuba1 mutant mouse
    Tamio Furuse
    Technology and Development Team for Mouse Phenotype Analysis, Japan Mouse Clinic, RIKEN BioResource Center, 3 1 1 Koyadai, Tsukuba, Ibaraki 305 0074, Japan
    Behav Brain Res 227:167-74. 2012
    ..The results of a behavioral pharmacological analysis using methylphenidate and morphological analyses of embryonic and adult brains suggested that Tuba1(Rgsc1736) is a novel animal model for neurodevelopmental disorders...
  70. ncbi Sequence characterization of ENU-induced mutants of glucose phosphate isomerase in mouse
    S R Pearce
    Wellcome Research Laboratory for Molecular Genetics, UMDS, Guy s Hospital, London, UK
    Mamm Genome 6:858-61. 1995
    ..The fourth identified mutation, Gpi1-sbm2H, is a deletion of amino acid residues Arg134 to Leu162 inclusive, which may arise from incorrect splicing of mRNA; a fifth mutation has remained undetermined...
  71. pmc hph-1: a mouse mutant with hereditary hyperphenylalaninemia induced by ethylnitrosourea mutagenesis
    V C Bode
    Division of Biology, Kansas State University, Manhattan 66506
    Genetics 118:299-305. 1988
    b>Ethylnitrosourea mutagenesis of spermatogonial stem cells and a three-generation breeding scheme were used to screen for recessive mutations that cause defects in phenylalanine metabolism leading to elevated serum levels of this amino ..
  72. pmc Discovery of candidate disease genes in ENU-induced mouse mutants by large-scale sequencing, including a splice-site mutation in nucleoredoxin
    Melissa K Boles
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA
    PLoS Genet 5:e1000759. 2009
    ..Our data show that the classical positional mapping approach of disease mutation identification can be extended to large target regions using high-throughput sequencing...
  73. ncbi Next-generation gene targeting in the mouse for functional genomics
    Yoichi Gondo
    Mutagenesis and Genomics Team, RIKEN BioResource Center, 3 1 1 Koyadai, Tsukuba, Ibaraki 305 0074, Japan
    BMB Rep 42:315-23. 2009
    ....
  74. ncbi Analysis of the MCMV resistome by ENU mutagenesis
    Karine Crozat
    Department of Immunology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, California 92037, USA
    Mamm Genome 17:398-406. 2006
    ....
  75. ncbi [Tumour induction by ethylnitrosourea in the central nervous system]
    S Bulnes-Sesma
    Laboratorio de Neurociencias Clinicas y Experimentales LaNCE, Departamento de Neurociencias, Universidad del Pais Vasco, Espana
    Rev Neurol 43:733-8. 2006
    ..Experimental central nervous system (CNS) tumours have been proposed as a useful model for the study of oncogenesis, epiphenomena related to cancer and for the design of new therapeutic strategies...
  76. ncbi Forward genetic analysis of TLR-signaling pathways: an evaluation
    Kasper Hoebe
    Cincinnati Children s Hospital Research Foundation, University of Cincinnati College of Medicine, Division of Molecular Immunology, Room S5 421, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Adv Drug Deliv Rev 60:824-9. 2008
    ..Here we review some of the more important insights developed from this approach and discuss its prospects...
  77. ncbi Two zebrafish mutants, ebony and ivory, uncover benefits of neighborhood on photoreceptor survival
    Paul Goldsmith
    Department of Anatomy, University of Cambridge, Downing Site, Cambridge, CB2 3DY, United Kingdom
    J Neurobiol 57:235-45. 2003
    ..The rescue at a distance is in keeping with a putative diffusible survival factor. We propose a density-dependent nonautonomous neighborhood effect to explain these findings...
  78. pmc Sequence analysis of N-ethyl-N-nitrosourea-induced vermilion mutations in Drosophila melanogaster
    A Pastink
    Department of Radiation Genetics, State University of Leiden, The Netherlands
    Genetics 123:123-9. 1989
    ..Although the latter arise later during development, the spectrum of mosaic mutations is also dominated by GC----AT transition mutations...
  79. ncbi Immunotherapeutic effects of T11TS/S-LFA3 against nitrosocompound mediated neural genotoxicity
    Joydeep Mukherjee
    Department of Physiology, Cellular and Molecular Immunology Lab, Dr B C Roy Post Graduate Institute of Basic Medical Sciences, Institute of Post Graduate Medical Education and Research, 244 B, J C Bose Road, Kolkata 700020, West Bengal, India
    Toxicol Lett 150:239-57. 2004
    ..Finally, formation of DNA ladder along with the FACS-based apoptosis study clearly indicated that T11TS is a potent apoptotic inducer in neoplastic neural cells...
  80. pmc An N-ethyl-N-nitrosourea mutagenesis recessive screen identifies two candidate regions for murine cardiomyopathy that map to chromosomes 1 and 15
    Liliana Fernandez
    Department of Medicine, Duke University Medical Center, DUMC, Durham, NC 27710, USA
    Mamm Genome 20:296-304. 2009
    ..The identification of the genes responsible for the observed phenotype in these families will be strong candidates for disease-causing or disease-modifying genes in patients with heart failure...
  81. pmc NG2-expressing glial precursor cells are a new potential oligodendroglioma cell initiating population in N-ethyl-N-nitrosourea-induced gliomagenesis
    Anne Briançon-Marjollet
    Inserm U873, 38054 Grenoble, France
    Carcinogenesis 31:1718-25. 2010
    ..This work may open up new perspectives for understanding biology of human gliomas...
  82. pmc Molecular analysis of mutations induced in the vermilion gene of Drosophila melanogaster by methyl methanesulfonate
    M J Nivard
    MGC Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, Sylvius Laboratories, The Netherlands
    Genetics 131:673-82. 1992
    ..A comparison of the mutational spectra of MMS with that produced by ethylnitrosourea (ENU), also in the vermilion locus of Drosophila, reveals major differences: predominantly transition ..
  83. ncbi Ethylnitrosourea-induced thymus-defective mutants identify roles of KIAA1440, TRRAP, and SKIV2L2 in teleost organ development
    Norimasa Iwanami
    Division of Experimental Immunology, Institute for Genome Research, University of Tokushima, Tokushima, Japan
    Eur J Immunol 39:2606-16. 2009
    ..To identify the genes that regulate thymus development, we previously carried out a large-scale screening for ethylnitrosourea-induced mutagenesis using medaka, Oryzias latipes, and established a panel of recessive thymus-lacking ..
  84. ncbi In vivo repair of ENU-induced oxygen alkylation damage by the nucleotide excision repair mechanism in Drosophila melanogaster
    L Tosal
    Departamento de Biologia Funcional e Instituto Universitario de Oncologia del Principado de Asturias, Universidad de Oviedo, Spain
    Mol Genet Genomics 265:327-35. 2001
    ....
  85. pmc An ENU-induced recessive mutation in Mpl leads to thrombocytopenia with overdominance
    E Ricky Chan
    Department of Genetics, Case Western Reserve University, Cleveland, Ohio 44106 4955, USA
    Exp Hematol 37:276-84. 2009
    ..The aim of this study was to identify and characterize the causative mutation in the thrombocytopenic mouse strain HLB219 that was generated at the Jackson Laboratory as part of a large-scale N-ethyl-N-nitrosourea mutagenesis screen...
  86. ncbi Features and strategies of ENU mouse mutagenesis
    Dian Soewarto
    Charite University Medicine Berlin, Molecular Cancer Research Center, Augustenburger Platz 1, 13393 Berlin, Germany
    Curr Pharm Biotechnol 10:198-213. 2009
    ..Finally, we provide information about the accessibility and availability of these models for academic research...
  87. ncbi Trends in large-scale mouse mutagenesis: from genetics to functional genomics
    Yoichi Gondo
    Mutagenesis and Genomics Team, RIKEN BioResource Center, 3 1 1 Koyadai, Tsukuba, Ibaraki, Japan
    Nat Rev Genet 9:803-10. 2008
    ..This article traces the origins and rationale of these large-scale mouse mutagenesis programmes...
  88. ncbi A gene-driven approach to the identification of ENU mutants in the mouse
    Emma L Coghill
    MRC Mammalian Genetics Unit and UK Mouse Genome Centre, Harwell OX11 ORD, UK
    Nat Genet 30:255-6. 2002
    ..We provide support for this approach and report the identification of mutations in the gene (Gjb2) encoding connexin 26, using archives established from the UK ENU mutagenesis program...
  89. ncbi Neural precursor cell apoptosis and glial tumorigenesis following transplacental ethyl-nitrosourea exposure
    J R Leonard
    Department of Pathology and Immunology, Division of Neuropathology, Washington University School of Medicine, St. Louis, MO 63110, USA
    Oncogene 20:8281-6. 2001
    ..These results suggest that intrauterine exposure of NPCs to certain DNA damaging agents may synergistically interact with specific genetic abnormalities (e.g. p53 deficiency) to produce glial neoplasms in the adult brain...
  90. ncbi Ethylnitrosourea induces apoptosis and growth arrest in the trophoblastic cells of rat placenta
    Kei Ichi Katayama
    Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Biol Reprod 67:431-5. 2002
    b>Ethylnitrosourea (ENU), a well known alkylating agent, induces congenital anomalies in fetuses when it is administered to pregnant animals...
  91. ncbi The mutagenic activity of ethylnitrosourea at low doses in spermatogonia of the mouse as assessed by the specific-locus test
    J Favor
    Institute of Mammalian Genetics, GSF Research Center for Environment and Health, D 85758, Neuherberg, Germany
    Mutat Res 405:221-6. 1998
    b>Ethylnitrosourea is the most efficient chemical mutagen in spermatogonial stem cells of the mouse and its mutagenic activity has been intensively studied...
  92. ncbi Genetic dissection of Toll-like receptor signaling using ENU mutagenesis
    Kasper Hoebe
    Department of Genetics, The Scripps Research Institute, La Jolla, CA 92037, USA
    Methods Mol Biol 517:239-51. 2009
    ..In this chapter I provide a practical guide for performing germline mutagenesis in mice. I focus on the application of this technology to the identification of genes involved in TLR signaling...
  93. ncbi Analysis of spontaneous, gamma ray- and ethylnitrosourea-induced hprt mutants in HL-60 cells with multiplex PCR
    Sheng Xue Liu
    Department of Healath Toxicology, Preventive Medical College, Third Military Medical University, Chongqing 400038, China
    World J Gastroenterol 9:578-83. 2003
    ..To explore the molecular spectra and mechanism of human hypoxanthine guanine phosphoribosyl transferase (hprt) gene mutation induced by ethyluitrosourea (ENU) and (60)Co gamma-rays...
  94. ncbi A genetic screen for mouse mutations with defects in serotonin responsiveness
    Karin C Weiss
    Samuel Lunenfeld Research Institute, Mt Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada M5G 1X5
    Brain Res Mol Brain Res 115:162-72. 2003
    ..Thus, it is well worth expanding on this genetic screen in its current form and by addition of further pharmacologic assays in the future...
  95. ncbi Target-selected inactivation of the zebrafish rag1 gene
    Erno Wienholds
    Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, Netherlands
    Science 297:99-102. 2002
    ..As sperm samples from all 2679 F1 fish were collected and cryopreserved, we have in principle generated a mutant library from which mutants of most zebrafish genes can be isolated...
  96. ncbi A gene-driven ENU-based approach to generating an allelic series in any gene
    Mohamed Mohideen Quwailid
    MRC Mammalian Genetics Unit, Medical Research Council, OX11 0RD, Harwell, Oxfordshire, UK
    Mamm Genome 15:585-91. 2004
    ..It is now available to academic collaborators as a community resource...
  97. ncbi Molecular characterization of ENU mouse mutagenesis and archives
    Yoshiyuki Sakuraba
    RIKEN Genomic Sciences Center, 1 7 22 Suehiro cho, Tsurumi ku, Yokohama 230 0045, Japan
    Biochem Biophys Res Commun 336:609-16. 2005
    ..The base-substitution spectra in this study were different from those of the phenotype-based mutagenesis. The ENU-based gene-driven mutagenesis in the mouse now becomes feasible and practical...
  98. ncbi Estimating the number of coding mutations in genotypic- and phenotypic-driven N-ethyl-N-nitrosourea (ENU) screens
    David A Keays
    Psychiatric Genetics Laboratory, Wellcome Trust Centre for Human Genetics, University of Oxford, UK
    Mamm Genome 17:230-8. 2006
    ..002). Where mutants are identified by genotypic methods, we show that backcrossing in the absence of marker-assisted selection is an inefficient means of eliminating linked confounding mutations...
  99. pmc Phenotype-based identification of mouse chromosome instability mutants
    Naoko Shima
    The Jackson Laboratory, Bar Harbor, Maine 04609, USA
    Genetics 163:1031-40. 2003
    ....
  100. pmc Mutation rate and predicted phenotypic target sizes in ethylnitrosourea-treated mice
    Dorothy Concepcion
    University of California San Diego School of Medicine, San Diego, California 92093 0644, USA
    Genetics 168:953-9. 2004
    ..To address these questions, we sequenced approximately 9.6 Mb of DNA from G1 progeny of ethylnitrosourea-treated mice in a large, broad-spectrum screen...
  101. pmc Random mutagenesis of the mouse genome: a strategy for discovering gene function and the molecular basis of disease
    Nhung Nguyen
    Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, Australia
    Am J Physiol Gastrointest Liver Physiol 300:G1-11. 2011
    ..This review examines the strategies and tools used in ENU mutagenesis screens to efficiently generate and identify functional mutations...

Research Grants62

  1. CLONING OF THE Hol MUTATION
    Licia Selleri; Fiscal Year: 2009
    ..To this end, we are performing a phenotype- based forward genetic screen by ethylnitrosourea (ENU) mutagenesis in the mouse to uncover novel genes important in mammalian craniofacial development...
  2. Modeling the genetic basis for human congenital heart disease in mice
    Cecilia W Lo; Fiscal Year: 2013
    ..We propose a complementary approach with forward genetic screening with ethylnitrosourea mutagenesis to recover mutations causing CHD...
  3. GENOME FUNCTION IN MOUSE WITH COMBINATION MUTAGENESIS
    John Schimenti; Fiscal Year: 2009
    ..Under previous funding, mutations were induced by randomly mutagenizing the genome with ethylnitrosourea (ENU), and selecting those on proximal Chr 5 using a breeding scheme that exploited a chromosome inversion ..
  4. Ubiquitination in erythropoiesis and the pathophysiology of anemia
    Mark D Fleming; Fiscal Year: 2013
    ..We have characterized the murine hem9 mutation from a systematic ethylnitrosourea screen for hematological mutants. hem9 homozygotes have hypochromic microcytic anemia...
  5. Dissecting Genetic Mechanisms of Hypertrophic Cardiomyopathy by ENU Mutagenesis
    Ferhaan Ahmad; Fiscal Year: 2012
    ..The NHLBI has funded a mouse ethylnitrosourea (ENU) mutagenesis program that is identifying genetic pathways contributing to congenital heart disease...
  6. Role of intrinsic apoptotic pathway in therapy-related myelodysplastic syndrome
    ELISE PETERSON LU; Fiscal Year: 2013
    ....
  7. Mutagenetic analysis of LPS responses
    BRUCE A BEUTLER; Fiscal Year: 2013
    ..Moreover, by disclosing points of host vulnerability during infection, it may suggest pharmacotherapeutic interventions helpful to all patients with sepsis. ..
  8. Study of the Genetic Basis for Fibrotic Diseases in a Mouse Model
    Weikuan Gu; Fiscal Year: 2013
    ..in the offspring of a male from the 101/H mouse strain as a result of administration of the mutagenic agent, ethylnitrosourea. Studies indicated that excess collagen production was the result of an increased number of collagen ..
  9. New Mouse Mutation Exhibiting Failed Fertilization
    Mary Ann Handel; Fiscal Year: 2006
    ..Thus, an expeditious and focused attack over a relatively short funding period is desirable to bring the project to the point of setting priorities for future research and development of the concept. ..
  10. Polyclonal Intestinal Tumors: Formation, Progression, and Significance
    RICHARD BROTT HALBERG; Fiscal Year: 2013
    ..We will analyze tumors from mice treated with either ethylnitrosourea (ENU) or azoxymethane (AOM), mice in which Apc is inactivated somatically by silencing, and mice in which ..
  11. Morphogenesis of the peripheral sensory nervous system
    MARK VOIGT; Fiscal Year: 2009
    ..We propose using these transgenic fish in a ethylnitrosourea-based mutagenesis screen in an effort to identify genes involved in any of the following three processes: ..
  12. Genetic Basis of Myeloid Neoplasia--Mutagenesis Screens
    A Look; Fiscal Year: 2005
    ..This proposal tests two linked hypotheses: (i) genome-wide ethylnitrosourea (ENU) mutagenesis screens in the zebrafish can be used to identify dominant and recessive mutations that ..
  13. Neuroblastoma Tumor Suppressor Genes in Zebrafish
    A Look; Fiscal Year: 2007
    ..This proposal tests two linked hypotheses: (i) genome-wide ethylnitrosourea (ENU) mutagenesis screens in the zebrafish can be used to identify dominant and recessive mutations that ..
  14. MOLECULAR ANALYSIS OF CARCINOGEN-INDUCED MUTATIONS
    Norman Drinkwater; Fiscal Year: 1992
    ....
  15. DIFFERENTIATION AND STROMA-INDUCTION IN NEURAL TUMORS
    LUCIEN RUBINSTEIN; Fiscal Year: 1980
    ..The tumors investigated include human neoplasms; experimental rat tumors obtained following transplacental ethylnitrosourea (ENU); an established glial rat tumor cell line (C-6); and the neuroepithelial component of a transplantable ..
  16. CHEMICAL INDUCTION OF PROSTATIC ADENOCARCINOMAS
    Maarten Bosland; Fiscal Year: 1993
    ..This is followed by a single treatment with methylnitrosourea (NMU) or ethylnitrosourea (ENU) during the maximum of the cell proliferative activity...
  17. RECEPTOR MUTANTS IN VITAMIN D RESISTANT RICKETS OF BONE
    Mark Hughes; Fiscal Year: 1991
    ..DNA contact points will be determined by alkylation interference experiments using dimethylsulfate and ethylnitrosourea. Similarly, mutations involving hormone binding affinity will be studied to define in molecular terms the ..
  18. SATURATION MUTAGENESIS OF MOUSE GENOMIC REGIONS
    Eugene Rinchik; Fiscal Year: 1992
    ....
  19. ANALYSIS OF DENDRIMER BASED MRI CONTRAST AGENTS
    Erik Wiener; Fiscal Year: 1999
    ..5 and 4.7T. This information will enable the rational design of effective passive or antigen directed MR contrast agents for the diagnosis of breast cancer and other pathologies. ..
  20. MUTANT LINES OF ZEBRAFISH HIGHLY SENSITIVE TO NEOPLASIA
    Jan Spitsbergen; Fiscal Year: 2005
    ..These lines of zebrafish will provide an efficient and inexpensive system for development of new treatment and prevention strategies for cancer, and will help identify new genes controlling growth and differentiation. ..
  21. EARLY CHANGES IN POTENTIAL BRAIN TUMOR TISSUE
    SHIRLEY SOUKUP; Fiscal Year: 1980
    b>Ethylnitrosourea (ENU) is a powerful transplacental carcinogen. A single dose to a pregnant rat produces neural tumors in adult offspring many months later...
  22. Genetics of rest:activity behavior in the mouse
    Maja Bucan; Fiscal Year: 2006
    ..Human orthologs of loci defined by these single gene mutations may represent additive or interactive contributions to the polygenic component of inherited psychiatric and sleep disorders. ..
  23. An ENU Mutagenesis Screen for Genes of Addiction
    AMY RAMSEY; Fiscal Year: 2009
    ..Saturation mutagenesis is achieved in the mouse with the alkylating agent ethylnitrosourea (ENU), which induces base pair mutations at high frequency...
  24. MOUSE GENETIC MODELS FOR PHENYLKETONURIA
    William Dove; Fiscal Year: 1993
    Potent mouse germline mutagenesis by ethylnitrosourea (ENU) is being combined with efficient breeding and screening protocols to generate an extended series of viable mouse mutants deficient in phenylalanine clearance...
  25. IDENTIFICATION OF MODIFIER GENES AFFECTING NEOPLASIA
    ANDREW THLIVERIS; Fiscal Year: 2004
    ..Strategies will search for both dominant and recessive tumor modifiers. A random germline approach using ethylnitrosourea (ENU) mutagenesis will be employed to search for such mutations...
  26. Flow cytometry-based Unscheduled DNA Synthesis (FLUDS)
    GEORGE DEGEORGE; Fiscal Year: 2005
    ..It has reliably predicted the UDS induced by ethylnitrosourea, aphidicolin, 2- acetylaminofluorene, and fluorene...
  27. SPONTANEOUS AND GENOTOXICANT INDUCED MUTATION MECHANISMS
    RICHARD SINDEN; Fiscal Year: 2003
    ..of numerous chemical and environmental genotoxicants, including UV irradiation, X-radiation, mitomycin C, ethylnitrosourea (ENU) and ethylmethane sulfonate (EMS), cisplatin, N-methyl-N'-nitro N-nitrosoguanidine (MNNG), polycyclic ..
  28. Targeting ATF5 for intervention in brain cancer
    ANGELO ARIAS; Fiscal Year: 2009
    ..will be created by retroviral injection of PDGF to demonstrate an amplified growth factor pathway, and by ethylnitrosourea (END) to simulate environmental exposure to a carcinogen...
  29. PHENOTYPIC SCREENS FOR CARDIOVASCULAR MUTATIONS IN MICE
    Howard Rockman; Fiscal Year: 2003
    ..to develop and experimentally validate a high throughput phenotype screening procedure, that when linked to a ethylnitrosourea (ENU) mutagenesis or a random gene-targeted program will: (1) detect mutations in genes previously ..
  30. GENETICS OF SLEEP AND REST BEHAVIOR IN THE MOUSE
    Maja Bucan; Fiscal Year: 2000
    ..end of description) ..
  31. BRONCHIOLAR CLARA CELLS: A ROLE IN TYPE 2 CELL FUNCTION
    Kenneth Palmer; Fiscal Year: 1990
    ..period, our laboratory has developed a unique approach to this problem by establishing and characterizing an ethylnitrosourea-induced (ENU) murine lung adenoma model, whose cells possess most of the structural and known biochemical ..
  32. Allelic Series of Genomic Modifications in ES Cells
    Terry Magnuson; Fiscal Year: 2006
    ....
  33. Sphinx: a new cause of hepatic neoplasia
    Kasper Hoebe; Fiscal Year: 2009
    ..In addition, using a unique mouse model identified in our laboratory, we aim to study the involvement of the immune system in the pathophysiology of hepatocellular tumor development. ..
  34. RANDOM MUTAGENESIS SCREEN FOR BEHAVIORAL MUTANTS IN MICE
    Maja Bucan; Fiscal Year: 2002
    ....
  35. INVESTIGATION OF ANGIOGENIC FUNCTION IN CANCER BY MRI
    Orhan Nalcioglu; Fiscal Year: 2001
    ..All of these goals are major issues in health care of breast-cancer patients and the successful outcome of the proposed investigation will help reduce the human and economic costs of breast cancer. ..
  36. Phenotypic screen in mouse embryonic for TGF-beta signaling mutations
    JAY VIVIAN; Fiscal Year: 2007
    ..Mutagenesis will be accomplished with ethylnitrosourea, a highly effective mutagen in ES cells...
  37. PIM-1 TRANSGENIC MICE TO DETECT ENVIRONMENTAL TOXINS
    David Jacobson Kram; Fiscal Year: 1992
    ..Eu-pim-1 mice treated with the model mutagen/carcinogen ethylnitrosourea are approximately 25-fold more sensitive to the development of lymphomas than are control mice (17)...
  38. STUDIES IN NEURO-ONCOGENESIS AND NEUROGENESIS
    IAN ZAGON; Fiscal Year: 1980
    ..This research will delineate the teratogenic action of ENU, define the role of neurogenesis during chemical carcinogenesis, and explore the etiology and pathogenesis of brain tumors. ..
  39. Genes that Mediate Tolerance to the Placental Allograft
    Bruce Beutler; Fiscal Year: 2006
    ..Fourth, circumvention of the toleragenic mechanism might be used to prevent normal gestation, leading to termination of pregnancy within a few days following implantation of the blastocyst. ..
  40. Mutagenic analysis of LPS responses
    Bruce Beutler; Fiscal Year: 2006
    ..In the case of mutations that abolish LPS tolerance, we will attempt to determine the net impact on host resistance to infection. ..
  41. Forward Genetic Analysis of the Innate Immune Contribution to MCMV Resistance
    Bruce Beutler; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  42. TLR4 as an LPS sendor and susceptibility locus
    Bruce Beutler; Fiscal Year: 2007
    ..The new mutations that are found--and those that have already been found in preliminary efforts--will be isolated by positional cloning. ..
  43. In vivo mutation assay based on pig-a locus
    Stephen Dertinger; Fiscal Year: 2004
    ....
  44. Studies on Influenza B Virus BM2 Protein Ion Channel
    LAWRENCE PINTO; Fiscal Year: 2009
    ....
  45. Measuring Cytogenetic Damage in Human Blood
    Stephen Dertinger; Fiscal Year: 2003
    ..abstract_text> ..
  46. Rapid Screen for Genotoxicants, Chemoprotectors, and Radioprotectors
    Stephen Dertinger; Fiscal Year: 2008
    ..In addition, this assay can be used to investigate agents designed to ameliorate the effects of radiation exposure, e.g. biological response modifiers. [unreadable] [unreadable] [unreadable] [unreadable]..
  47. High Throughput Radiation Biodosimetry
    Stephen Dertinger; Fiscal Year: 2007
    ..We propose to develop such a radiation biodosimetry assay based on the extremely high analysis rates that are possible with flow cytometry instrumentation. [unreadable] [unreadable] [unreadable]..
  48. Versatile Mutation Assay Platform
    Stephen Dertinger; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  49. RESPIRATORY VIRUS ION CHANNELS
    LAWRENCE PINTO; Fiscal Year: 2007
    ..5. In a separate set of experiments we will use a sensitive method to test the NB protein of influenza B virus for ion channel activity. [unreadable] [unreadable] [unreadable]..
  50. Automation of the vitro Micronucleus Assay
    Stephen Dertinger; Fiscal Year: 2004
    ..abstract_text> ..
  51. High Throughput Assays for Ion Channel Activities of Influenza A & B Viruses
    LAWRENCE PINTO; Fiscal Year: 2006
    ..These assays will make it possible to perform efficient searches for antiviral drugs against the M2 ion channels. [unreadable] [unreadable] [unreadable] [unreadable]..
  52. Mutagenesis of the Mammalian Imprinting Process(es)
    Eugene Rinchik; Fiscal Year: 2002
    ..abstract_text> ..
  53. MUTATIONAL MECHANISMS OF REPETITIVE DNA IN HUMAN CELLS
    Kristin Eckert; Fiscal Year: 2001
    ..Our long-term research objective is to test the hypothesis that mutations in repetitive DNA provide an important source of genotypic variation that drives neoplastic progression. ..
  54. Involvement of a K+ Channel in C. elegans Male Behavior
    ELIZABETH DESTASIO; Fiscal Year: 2005
    ..Lastly, this research will help delineate the connections between motility, functions of particular muscle cells, and organismal behavior. ..
  55. DNA replication, DNA repair and microsatellite stability
    Kristin Eckert; Fiscal Year: 2009
    ..Microsatellite allele lengths can directly affect gene expression. As microsatellites are polymorphic in human populations, this effect on gene regulation may be an important factor contributing to individual cancer risk. ..
  56. CHARACTERIZATION OF THE MURINE WAVED 3 MUTATION
    David Beier; Fiscal Year: 2004
    ..The combination of a positional cloning approach with a detailed characterization of the wa3 phenotype should provide insight into understanding genes that control normal skin development. ..
  57. Creation of mouse strains for the study of hematopoiesis
    Archibald Perkins; Fiscal Year: 2004
    ..The establishment of such strains will allow one to screen for mutants that have abnormal hematopoiesis. ..
  58. Mouse Mutagenesis Workshops
    David Beier; Fiscal Year: 2006
    ..Ultimately, this workshop will serve the goal of improving public health by facilitating the generation and utilization of model systems for biomedical investigation. ..
  59. Alcohol selected mice: Comparative withdrawal syndromes
    John Belknap; Fiscal Year: 2008
    ..We will use the newly available Affymetrix mouse 430 2.0 Genechip(r) representing the vast majority of all genes in the mouse genome. [unreadable] [unreadable]..
  60. Analysis of Genetic Modifiers of PKD in the Mouse
    David Beier; Fiscal Year: 2008
    ..It is therefore an aim of this proposal to develop mutant mice that develop cysts as a consequence of loss of heterozygosity, using either a spontaneous or inducible system. ..