Genomes and Genes
Summary: A fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity.
Publications299 found, 100 shown here
- Diacylglycerol is required for the formation of COPI vesicles in the Golgi-to-ER transport pathwayInés Fernández-Ulibarri
Departament de Biologia Cellular i Anatomia Patologica, Facultat de Medicina and Institut d Investigacions Biomèdiques August Pi i Sunyer, Universitat de Barcelona, 08036 Barcelona, Spain
Mol Biol Cell 18:3250-63. 2007..Our findings implicate diacylglycerol in the retrograde transport of proteins from Golgi to the ER and suggest that it plays a critical role at a late stage of COPI vesicle formation...
- The Arabidopsis GNOM ARF-GEF mediates endosomal recycling, auxin transport, and auxin-dependent plant growthNiko Geldner
ZMBP, Entwicklungsgenetik, Universitat Tubingen, Auf der Morgenstelle 3, D 72076, Tubingen, Germany
Cell 112:219-30. 2003..The Arabidopsis protein GNOM is a brefeldin A (BFA) sensitive ARF-GEF that is required for the proper polar localization of PIN1, a candidate transporter of ..
- Synaptic vesicle recycling at CNS snapses without AP-2Sung Hyun Kim
Department of Biochemistry, Weill Cornell Medical College, New York, New York 10065, USA
J Neurosci 29:3865-74. 2009..These results demonstrate that in the absence of AP-2, SV proteins still become endocytosed, and synaptic vesicle recycling remains operational...
- Human papillomavirus type 16 infection of human keratinocytes requires clathrin and caveolin-1 and is brefeldin a sensitiveValerie Laniosz
Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064, USA
J Virol 83:8221-32. 2009..An understanding of the steps involved in HPV16 sorting and trafficking opens up the possibility of developing novel approaches to interfere with HPV16 infection and reduce the burden of papillomavirus diseases including cervical cancer...
- bfr1+, a novel gene of Schizosaccharomyces pombe which confers brefeldin A resistance, is structurally related to the ATP-binding cassette superfamilyK Nagao
Department of Biotechnology, University of Tokyo, Japan
J Bacteriol 177:1536-43. 1995..a Schizosaccharomyces pombe gene, bfr1+, which on a multicopy plasmid vector, pDB248', confers resistance to brefeldin A (BFA), an inhibitor of intracellular protein transport...
- Crystal structure of ARF1*Sec7 complexed with Brefeldin A and its implications for the guanine nucleotide exchange mechanismElena Mossessova
Howard Hughes Medical Institute, Structural Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
Mol Cell 12:1403-11. 2003..b>Brefeldin A (BFA) is a fungal metabolite that binds to the ARF1*GDP*Sec7 complex and blocks GEF activity at an early stage ..
- GBF1, a guanine nucleotide exchange factor for ADP-ribosylation factors, is localized to the cis-Golgi and involved in membrane association of the COPI coatKazumasa Kawamoto
Institute of Biological Sciences and Gene Research Center, University of Tsukuba, Tsukuba Science City, Ibaraki 305 8572, Japan
Traffic 3:483-95. 2002..This process is blocked by brefeldin A, which is an inhibitor of guanine nucleotide exchange factors for ADP-ribosylation factor...
- Brefeldin A: deciphering an enigmatic inhibitor of secretionAndreas Nebenführ
Department of Botany, University of Tennessee, Knoxville, Tennessee 37996 1100, USA
Plant Physiol 130:1102-8. 2002
- Poliovirus proteins induce membrane association of GTPase ADP-ribosylation factorGeorge A Belov
Laboratory of Infectious Diseases, NIAID, NIH, Building 50, Room 6120, Bethesda, MD 20892 8011, USA
J Virol 79:7207-16. 2005..Replication of poliovirus is sensitive to brefeldin A (BFA), a fungal metabolite known to prevent normal function of the ADP-ribosylation factor (ARF) family of ..
- Intracellular localization and in vivo trafficking of p24A and p23R Blum
Institute of Physiology II, Medical Biochemistry and Department of Anatomy, University of the Saarland, D 66421 Homburg Saar, Germany
J Cell Sci 112:537-48. 1999..This study therefore gives new insights into the localization and trafficking behavior of the two COPI-binding proteins p24A and p23...
- An ARF-GEF acting at the Golgi and in selective endocytosis in polarized plant cellsOoi Kock Teh
Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK
Nature 448:493-6. 2007..at the Golgi but is also required for selective internalization from the plasma membrane in the presence of brefeldin A (BFA)...
- Purification and cloning of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP-ribosylation factorsA Togawa
Pulmonary Critical Care Medicine Branch, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 274:12308-15. 1999..Two major families of ARF GEPs are known: approximately 200-kDa molecules that are inhibited by brefeldin A (BFA), a fungal metabolite that blocks protein secretion and causes apparent disintegration of Golgi structure, ..
- Intracellular trafficking of Potato leafroll virus movement protein in transgenic ArabidopsisFlorian Vogel
Friedrich Alexander Universitat Erlangen Nurnberg, Staudtstrasse 5, 91058 Erlangen, Germany
Traffic 8:1205-14. 2007..Interestingly, inhibition of the 26S proteasome led to aggregation of MP17 in aggresome-like structures. Formation of these structures could be inhibited by colchicine, as was shown for aggresomes in mammalian cells...
- The endosomal system of plants: charting new and familiar territoriesDavid G Robinson
Heidelberg Institute of Plant Sciences, University of Heidelberg, 69120 Heidelberg, Germany
Plant Physiol 147:1482-92. 2008
- A GFP-based assay reveals a role for RHD3 in transport between the endoplasmic reticulum and Golgi apparatusHuanquan Zheng
Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK
Plant J 37:398-414. 2004..Inhibition of secGFP transport between the ER and plasma membrane (PM), either by Brefeldin A (BFA) treatment or by genetic intervention results in increased intracellular secGFP accumulation accompanied ..
- Brefeldin A sensitivity and resistance in Schizosaccharomyces pombe. Isolation of multiple genes conferring resistanceT G Turi
Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510
J Biol Chem 269:24229-36. 1994The fungal metabolite brefeldin A (BFA) causes the inhibition of protein secretion and the disruption of the structure and function of the Golgi complex in mammalian cells...
- Organization and dynamics of the Aspergillus nidulans Golgi during apical extension and mitosisAreti Pantazopoulou
Departamento de Microbiologia Molecular, Centro de Investigaciones Biologicas C S I C, Madrid 28040, Spain
Mol Biol Cell 20:4335-47. 2009..b>Brefeldin A transiently collapses late and early GEs into distinct aggregates containing Sec7/mRFP-PH(OSBP) and GrhA-GFP, ..
- Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46Lionel Breuza
Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
J Biol Chem 279:47242-53. 2004..we have developed a novel procedure for the purification of ERGIC membranes from HepG2 cells treated with brefeldin A, a drug known to accumulate cycling proteins in the ERGIC...
- PINOID positively regulates auxin efflux in Arabidopsis root hair cells and tobacco cellsSang Ho Lee
Department of Biology, Chungnam National University, Daejeon 305 764, Korea
Plant Cell 18:1604-16. 2006..Localization of PID or PIN3 at the cell boundary was disrupted by brefeldin A and staurosporine...
- Regulation of stability and function of the epithelial Na+ channel (ENaC) by ubiquitinationO Staub
Hospital for Sick Children, Division of Respiratory Research, Toronto, Ontario, Canada
EMBO J 16:6325-36. 1997..retention or recycling of ENaC and incorporation of new channels at the plasma membrane, as determined by Brefeldin A treatment...
- Munc18 interacting proteins: ADP-ribosylation factor-dependent coat proteins that regulate the traffic of beta-Alzheimer's precursor proteinKaren Hill
Department of Biochemistry, School of Medicine, Emory University, Atlanta, Georgia 30322 3050, USA
J Biol Chem 278:36032-40. 2003..We propose that MINTs are a family of Arf-dependent, vesicle-coat proteins that can regulate the traffic of beta-APP...
- Dissection of brefeldin A-sensitive and -insensitive steps in apicoplast protein targetingAmy DeRocher
Seattle Biomedical Research Institute, 307 Westlake Avenue N, Suite 500 Seattle, WA 98109, USA
J Cell Sci 118:565-74. 2005..Incubation with brefeldin A, which blocks ER-to-Golgi trafficking by inhibiting a GTP exchange factor required for retrograde trafficking, ..
- A critical role of a cellular membrane traffic protein in poliovirus RNA replicationGeorge A Belov
National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
PLoS Pathog 4:e1000216. 2008..Virus replication is inhibited by brefeldin A (BFA), implicating some components(s) of the cellular secretory pathway in virus growth...
- Functional diversification of closely related ARF-GEFs in protein secretion and recyclingSandra Richter
ZMBP, Entwicklungsgenetik, Tubingen, Germany
Nature 448:488-92. 2007....
- Dissection of membrane dynamics of the ARF-guanine nucleotide exchange factor GBF1Tomasz Szul
Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35924, USA
Traffic 6:374-85. 2005..of an inactive (E794K) GBF1 mutant, expression of the ARF1 (T31N) mutant with decreased affinity for GTP and Brefeldin A treatment], we show that GBF1 is stabilized on membranes when in a complex with ARF-GDP...
- Brefeldin A, but not monensin, completely blocks CD69 expression on mouse lymphocytes: efficacy of inhibitors of protein secretion in protocols for intracellular cytokine staining by flow cytometryS Nylander
Cell Biology and Biochemistry, Preclinical R and D, ASTRA Hässle, Molndal, Sweden
J Immunol Methods 224:69-76. 1999..The two most widely used reagents are monensin and brefeldin A (BFA). These reagents differ somewhat in their mode of action, which might explain their different effects...
- The brefeldin A resistance protein Bfr1p is a component of polyribosome-associated mRNP complexes in yeastB D Lang
Graduate Program in Biochemistry, Emory University School of Medicine, 1462 Clifton Road NE, Atlanta, GA 30322, USA
Nucleic Acids Res 29:2567-74. 2001The yeast gene BFR1 was originally isolated from a genetic screen for high-copy suppressors of brefeldin A-induced lethality in Saccharomyces cerevisiae...
- Partitioning of the matrix fraction of the Golgi apparatus during mitosis in animal cellsJoachim Seemann
Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, Post Office Box 208002, New Haven, CT 06520 8002, USA
Science 295:848-51. 2002..We fractionated the Golgi apparatus in vivo using the drug brefeldin A or a dominant-negative mutant of the Sar1p protein...
- Differential effects of the brefeldin A analogue (6R)-hydroxy-BFA in tobacco and ArabidopsisMarkus Langhans
Department of Cell Biology, Heidelberg Institute for Plant Sciences, University of Heidelberg, D 69120 Heidelberg, Germany
J Exp Bot 62:2949-57. 2011The effects of two brefeldin A (BFA) analogues (BFA lactam; 6(R)-hydroxy-BFA) on plant cells were tested...
- Ganglioside GD3 traffics from the trans-Golgi network to plasma membrane by a Rab11-independent and brefeldin A-insensitive exocytic pathwayPilar Maria Crespo
Centro de Investigaciones en Quimica Biologica de Cordoba, CIQUIBIC UNC CONICET, Departamento de Quimica Biologica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, 5000 Cordoba, Argentina
J Biol Chem 279:47610-8. 2004..did not partition into GEM domains in the Golgi complex and trafficked from TGN to plasma membrane by a brefeldin A-insensitive exocytic pathway...
- Involvement of caspase-2 and caspase-9 in endoplasmic reticulum stress-induced apoptosis: a role for the IAPsHerman H Cheung
Apoptosis Research Centre, Children s Hospital of Eastern Ontario Research Institute II, Ottawa, Ontario, Canada K1H 8L1
Exp Cell Res 312:2347-57. 2006..Here we show that brefeldin A and tunicamycin-mediated ER stress lead to caspase-dependent apoptosis involving caspase-2...
- The antibody to GD3 ganglioside, R24, is rapidly endocytosed and recycled to the plasma membrane via the endocytic recycling compartment. Inhibitory effect of brefeldin A and monensinRamiro Iglesias-Bartolomé
Centro de Investigaciones en Quimica Biologica de Cordoba, CIQUIBIC UNC CONICET, Departamento de Quimica Biologica, Universidad Nacional de Cordoba, Argentina
FEBS J 273:1744-58. 2006..exits the recycling compartment en route to the plasma membrane by a dynamin 2-dependent pathway sensitive to brefeldin A and monensin...
- The SPCA1 Ca2+ pump and intracellular membrane traffickingMassimo Micaroni
Laboratory of Intracellular Traffic, Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, Via Nazionale 8 A, 66030 Santa Maria Imbaro Chieti, Italy
Traffic 11:1315-33. 2010..and delays retrograde redistribution of the GA glycosylation enzymes into the endoplasmic reticulum caused by brefeldin A; however, exit of these enzymes from the endoplasmic reticulum is not affected...
- Transmembrane domain length determines intracellular membrane compartment localization of syntaxins 3, 4, and 5R T Watson
Department of Physiology and Biophysics, The University of Iowa, Iowa City, Iowa 52242, USA
Am J Physiol Cell Physiol 281:C215-23. 2001..These data support a model wherein short transmembrane domains (< or =17 amino acids) direct the cis-Golgi localization of syntaxins, whereas long transmembrane domains (> or =23 amino acids) direct plasma membrane localization...
- ADP-ribosylation factor 6 as a target of guanine nucleotide exchange factor GRP1S E Langille
Program in Molecular Medicine, Worcester, Massachusetts 01605, USA
J Biol Chem 274:27099-104. 1999..GTP loading of ARF6 in both control cells and in response to GRP1 or cytohesin-1 was insensitive to brefeldin A, consistent with previous data on endogenous ARF6 exchange activity...
- PDMP blocks brefeldin A-induced retrograde membrane transport from golgi to ER: evidence for involvement of calcium homeostasis and dissociation from sphingolipid metabolismJ W Kok
Department of Physiological Chemistry, University of Groningen, Groningen Institute for Drug Studies GIDS, 9713 AV Groningen, The Netherlands
J Cell Biol 142:25-38. 1998..of sphingolipid biosynthesis, D,L-threo-1-phenyl-2- decanoylamino-3-morpholino-1-propanol (PDMP), inhibits brefeldin A (BFA)-induced retrograde membrane transport from Golgi to endoplasmic reticulum (ER)...
- Localization and recycling of gp27 (hp24gamma3): complex formation with other p24 family membersJ Fullekrug
Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
Mol Biol Cell 10:1939-55. 1999..Second, treatment with brefeldin A caused gp27 to relocate into peripheral structures positive for both KDEL receptor and COPII...
- The ARF exchange factors Gea1p and Gea2p regulate Golgi structure and function in yeastA Peyroche
Service de Biochimie et Génétique Moléculaire, Bat 142, Departement de Biologie Cellulaire et Moleculaire, CEA Saclay, 91191 Gif sur Yvette, France
J Cell Sci 114:2241-53. 2001..In addition, the structure of Golgi elements was severely perturbed in gea mutants. We conclude that Gea1p and Gea2p play an important role in the structure and functioning of the Golgi apparatus in yeast...
- Isolation of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP ribosylation factor (ARF) 1 and ARF3 that contains a Sec7-like domainN Morinaga
Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 93:12856-60. 1996b>Brefeldin A (BFA) inhibited the exchange of ADP ribosylation factor (ARF)-bound GDP for GTP by a Golgi-associated guanine nucleotide-exchange protein (GEP) [Helms, J.B. & Rothman, J.E...
- Characterization of class I and II ADP-ribosylation factors (Arfs) in live cells: GDP-bound class II Arfs associate with the ER-Golgi intermediate compartment independently of GBF1Justin Chun
Department of Cell Biology, University of Alberta, Edmonton, AB T6G 2H7, Canada
Mol Biol Cell 19:3488-500. 2008..Live cell imaging detected Arfs on peripheral puncta that also contained Golgi-specific brefeldin A (BFA) resistance factor (GBF) 1 and the ERGIC marker p58...
- Overexpression of an ADP-ribosylation factor-guanine nucleotide exchange factor, BIG2, uncouples brefeldin A-induced adaptor protein-1 coat dissociation and membrane tubulationChisa Shinotsuka
Institute of Biological Sciences and Gene Research Center, University of Tsukuba, 1 1 1 Tennohdai, Tsukuba Science City, Ibaraki 305 8572, Japan
J Biol Chem 277:9468-73. 2002..A fungal metabolite, brefeldin A (BFA), inhibits ARF-GEFs and leads to redistribution of coat proteins from membranes to the cytoplasm and ..
- Identification of multivesicular bodies as prevacuolar compartments in Nicotiana tabacum BY-2 cellsYu Chung Tse
Department of Biology, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
Plant Cell 16:672-93. 2004..b>Brefeldin A induced the YFP-labeled Golgi stacks but not the YFP-marked PVCs to form typical enlarged structures...
- Accumulation of caveolin in the endoplasmic reticulum redirects the protein to lipid storage dropletsA G Ostermeyer
Department of Biochemistry and Cell Biology, State University of New York at Stony Brook, Stony Brook, New York 11794, USA
J Cell Biol 152:1071-8. 2001..Third, incubation of cells with brefeldin A caused caveolin-1 to accumulate in the droplets...
- Caveolin-2 is targeted to lipid droplets, a new "membrane domain" in the cellT Fujimoto
Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, Nagoya 466 8550, Japan
J Cell Biol 152:1079-85. 2001..b>Brefeldin A treatment induced further accumulation of caveolin-2 along with caveolin-1 in LD...
- Auxin transport inhibitors block PIN1 cycling and vesicle traffickingN Geldner
, , Auf der Morgenstelle 3, , Germany
Nature 413:425-8. 2001..In support of our conclusion, the vesicle-trafficking inhibitor brefeldin A mimics physiological effects of auxin transport inhibitors.
- Differential requirements for COPI coats in formation of replication complexes among three genera of PicornaviridaeElena V Gazina
Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria 3004, Australia
J Virol 76:11113-22. 2002..b>Brefeldin A (BFA) has been shown to strongly inhibit RNA replication of poliovirus but not of encephalomyocarditis virus (..
- BFA-induced compartments from the Golgi apparatus and trans-Golgi network/early endosome are distinct in plant cellsSheung Kwan Lam
Department of Biology and Molecular Biotechnology Program, Centre for Cell and Developmental Biology, State China Key Laboratory for Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
Plant J 60:865-81. 2009b>Brefeldin A (BFA) is a useful tool for studying protein trafficking and identifying organelles in the plant secretory and endocytic pathways...
- Trafficking of the human transferrin receptor in plant cells: effects of tyrphostin A23 and brefeldin AElena Ortiz-Zapater
Departamento de Bioquimica y Biologia Molecular, Facultad de Farmacia, Universidad de Valencia, Avda Vicente Andres Estelles s n, 46100 Burjassot Valencia, Spain
Plant J 48:757-70. 2006..In contrast, treatment with brefeldin A, which inhibits recycling from endosomes back to the plasma membrane in plant cells, leads to the accumulation ..
- Dynamics of GBF1, a Brefeldin A-sensitive Arf1 exchange factor at the GolgiTing Kuang Niu
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Mol Biol Cell 16:1213-22. 2005..The drug brefeldin A (BFA), an uncompetitive inhibitor of the exchange reaction that binds to an Arf-GDP-Arf GEF complex, stabilizes ..
- Effects of brefeldin A on the localization of Tobamovirus movement protein and cell-to-cell movement of the virusYuko Tagami
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba 3 8 1, Meguro, Tokyo 153 8902, Japan
Virology 361:133-40. 2007..Treatment with brefeldin A (BFA), which disrupts ER-to-Golgi transport, inhibited the formation of irregularly shaped and filamentous ..
- Exo1: a new chemical inhibitor of the exocytic pathwayYan Feng
Institute of Chemistry and Cell Biology, Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 100:6469-74. 2003..Like Brefeldin A (BFA), Exo1 induces the rapid release of ADP-ribosylation factor (ARF) 1 from Golgi membranes but has less ..
- The Intra- and intercellular movement of Melon necrotic spot virus (MNSV) depends on an active secretory pathwayAinhoa Genoves
Instituto Biologia Molecular y Celular de Plantas, Universidad Politecnica, Universidad Politecnica de Valencia CSIC, Avenida de los Naranjos s n, 46022 Valencia, Spain
Mol Plant Microbe Interact 23:263-72. 2010..Hence, the secretory pathway inhibitor, Brefeldin A (BFA), and the overexpression of the GTPase-defective mutant of Sar1p, Sar1[H74L], fully retained the p7B ..
- Brefeldin A acts to stabilize an abortive ARF-GDP-Sec7 domain protein complex: involvement of specific residues of the Sec7 domainA Peyroche
Service de Biochimie et Génétique Moléculaire, CEA Saclay, Gif sur Yvette, France
Mol Cell 3:275-85. 1999We demonstrate that the major in vivo targets of brefeldin A (BFA) in the secretory pathway of budding yeast are the three members of the Sec7 domain family of ARF exchange factors: Gea1p and Gea2p (functionally interchangeable) and ..
- Regulation of Arf activation: the Sec7 family of guanine nucleotide exchange factorsJames E Casanova
Department of Cell Biology, University of Virginia Health System, Charlottesville, VA 22908 0732, USA
Traffic 8:1476-85. 2007..Here we review recent progress in our understanding of the structure, localization and biology of the different classes of Arf GEFs...
- ADP-Ribosylation factor 1 (ARF1) regulates recruitment of the AP-3 adaptor complex to membranesC E Ooi
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health NIH, Bethesda, Maryland 20892, USA
J Cell Biol 142:391-402. 1998..Association of AP-3 with membranes in vitro was enhanced by GTPgammaS and inhibited by brefeldin A (BFA), an inhibitor of ARF1 guanine nucleotide exchange...
- Protein domains involved in assembly in the endoplasmic reticulum promote vacuolar delivery when fused to secretory GFP, indicating a protein quality control pathway for degradation in the plant vacuoleOmbretta Foresti
Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, via Bassini 15, 20133 Milano, Italy, EU
Mol Plant 1:1067-76. 2008..is delivered to the vacuole and fragmented, in a process that is inhibited by the secretory traffic inhibitor brefeldin A. Moreover, a fusion between GFP and a domain of the maize storage protein gamma-zein involved in zein ..
- Brefeldin A action and recovery in Chlamydomonas are rapid and involve fusion and fission of Golgi cisternaeE Hummel
Department of Cell Biology, Heidelberg Institute for Plant Sciences HIP, University of Heidelberg, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany
Plant Biol (Stuttg) 9:489-501. 2007..These structures then divide by vertical fission to produce Golgi stacks of normal size and morphology roughly 60 min after drug wash-out...
- Plant N-glycan processing enzymes employ different targeting mechanisms for their spatial arrangement along the secretory pathwayClaude Saint-Jore-Dupas
Centre National de la Recherche Scientifique, Unité Mixte de Recherche 6037, IFRMP 23, GDR 2590, UFR des Sciences, Universite de Rouen, 76821 Mont Saint Aignan Cedex, France
Plant Cell 18:3182-200. 2006..We conclude that the localization of N-glycan processing enzymes corresponds to an assembly line in the early secretory pathway and depends on both TMD length and signals in the cytoplasmic tail...
- p200 ARF-GEP1: a Golgi-localized guanine nucleotide exchange protein whose Sec7 domain is targeted by the drug brefeldin AS J Mansour
Department of Cell Biology, University of Alberta, MSB 5 14, Edmonton, AB T6G 2H7, Canada
Proc Natl Acad Sci U S A 96:7968-73. 1999The drug brefeldin A (BFA) disrupts protein traffic and Golgi morphology by blocking activation of ADP ribosylation factors (ARFs) through an unknown mechanism...
- Cell surface delivery and structural re-organization by pharmacological chaperones of an oligomerization-defective alpha(1b)-adrenoceptor mutant demonstrates membrane targeting of GPCR oligomersMeritxell Canals
Molecular Pharmacology Group, Neuroscience and Molecular Pharmacology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow G128QQ, Scotland, UK
Biochem J 417:161-72. 2009....
- Characterization of Aspergillus nidulans RabC/Rab6Areti Pantazopoulou
Department of Molecular and Cellular Medicine, Centro de Investigaciones Biologicas CSIC, Ramiro de Maeztu 9, Madrid 28040, Spain
Traffic 12:386-406. 2011..rabCΔ mutants, showing markedly impaired apical extension, have conspicuously fragmented, brefeldin A-insensitive early and late GEs, indicating that the Golgi network organization requires RabC...
- Proteomics on brefeldin A-treated Arabidopsis roots reveals profilin 2 as a new protein involved in the cross-talk between vesicular trafficking and the actin cytoskeletonTomáš Takáč
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Department of Cell Biology, Palacký University, Olomouc, Czech Republic
J Proteome Res 10:488-501. 2011..Here, proteomic and cell biology approaches were applied to study effects of brefeldin A (BFA), an inhibitor of vesicle recycling and secretion, in Arabidopsis roots...
- Epac mediates PACAP-dependent long-term depression in the hippocampusJeanne Ster
CNRS, UMR 5203, Institut de Genomique Fonctionnelle, Montpellier, France
J Physiol 587:101-13. 2009..Altogether these results provided evidence for a new form of hippocampal LTD...
- ADP-ribosylation factor 1 of Arabidopsis plays a critical role in intracellular trafficking and maintenance of endoplasmic reticulum morphology in ArabidopsisMi Hee Lee
Center for Plant Intracellular Trafficking, Pohang University of Science and Technology, Pohang, 790 784, Korea
Plant Physiol 129:1507-20. 2002..The treatment of protoplasts with brefeldin A had exactly the same effect as Arf1[T31N] on various intracellular trafficking pathways...
- Different effects of aluminum on the actin cytoskeleton and brefeldin A-sensitive vesicle recycling in root apex cells of two maize varieties differing in root elongation rate and aluminum toleranceMontse Amenós
Institut für Zelluläre und Molekulare Botanik, Universitat Bonn, Kirschallee 1, D 53115 Bonn, Germany
Plant Cell Physiol 50:528-40. 2009..Visualization of boron-cross-linked rhamnogalacturonan II (RGII)-containing brefeldin A (BFA) compartments revealed that Al inhibited the formation of these compartments, especially in variety 16x36...
- TNO1 is involved in salt tolerance and vacuolar trafficking in ArabidopsisSang Jin Kim
Department of Genetics, Iowa State University, Ames, Iowa 50011, USA
Plant Physiol 156:514-26. 2011..The tno1 mutant had delayed formation of the brefeldin A (BFA) compartment in cotyledons upon application of BFA, suggesting less efficient membrane fusion processes in ..
- Tunicamycin and Brefeldin A induce in plant cells a programmed cell death showing apoptotic featuresP Crosti
Dipartimento di Biotecnologie e Bioscienze, , Via Celoria, 26, I-20133 Milano, Italy
Protoplasma 216:31-8. 2001..In this paper we describe the effects of Tunicamycin, an inhibitor of N-linked protein glycosylation, and Brefeldin A, an inhibitor of protein trafficking from the Golgi apparatus, on sycamore (Acer pseudoplatanus L...
- Mouse hepatitis coronavirus RNA replication depends on GBF1-mediated ARF1 activationMonique H Verheije
Virology Division, Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands
PLoS Pathog 4:e1000088. 2008..We now show that MHV replication is sensitive to brefeldin A (BFA)...
- Evaluation of the primary effect of brefeldin A treatment upon herpes simplex virus assemblyA Dasgupta
Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
J Gen Virol 82:1561-7. 2001Addition of the drug brefeldin A (BFA) to cells infected by herpes simplex virus (HSV) type 1 is known to result in a complex pattern of defects in particle assembly...
- cis-Golgi proteins accumulate near the ER exit sites and act as the scaffold for Golgi regeneration after brefeldin A treatment in tobacco BY-2 cellsYoko Ito
Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo, Japan
Mol Biol Cell 23:3203-14. 2012..expressing multiple Golgi markers tagged by different fluorescent proteins and observed their responses to brefeldin A (BFA) treatment and BFA removal...
- BFR1, a multicopy suppressor of brefeldin A-induced lethality, is implicated in secretion and nuclear segregation in Saccharomyces cerevisiaeC L Jackson
Departement de Biologie Cellulaire et Moleculaire, Centre d Etudes de Saclay, Gif sur Yvette, France
Genetics 137:423-37. 1994b>Brefeldin A (BFA) blocks protein transport out of the Golgi apparatus and causes disassembly of this organelle in mammalian cells. The primary effect of BFA is the release of the non-clathrin coat from Golgi membranes and vesicles...
- Brefeldin A inhibits pestivirus release from infected cells, without affecting its assembly and infectivityAlina Macovei
Institute of Biochemistry, Splaiul Independentei, 296, Sector 6, Bucharest 77700, Romania
Biochem Biophys Res Commun 346:1083-90. 2006..Here, we analyzed the intracellular localization, N-glycan processing and secretion of BVDV using brefeldin A (BFA), which blocks protein export from the endoplasmic reticulum (ER) and causes disruption of the Golgi ..
- Distinct membrane domains on endosomes in the recycling pathway visualized by multicolor imaging of Rab4, Rab5, and Rab11B Sönnichsen
Max Planck Institute for Molecular Cell Biology and Genetics, 01307 Dresden, Germany
J Cell Biol 149:901-14. 2000..We propose that endosomes are organized as a mosaic of different Rab domains created through the recruitment of specific effector proteins, which cooperatively act to generate a restricted environment on the membrane...
- Arf3 is activated uniquely at the trans-Golgi network by brefeldin A-inhibited guanine nucleotide exchange factorsFlorin Manolea
Department of Cell Biology, University of Alberta, Edmonton, AB, Canada
Mol Biol Cell 21:1836-49. 2010..These results suggest that Arf3 plays a unique function at the TGN that likely involves recruitment by a specific receptor...
- Identification of the protein storage vacuole and protein targeting to the vacuole in leaf cells of three plant speciesMisoon Park
Center for Plant Intracellular Trafficking, Pohang University of Science and Technology, Pohang, 790 784, Korea
Plant Physiol 134:625-39. 2004..In Arabidopsis, phaseolin contained N-glycans modified by Golgi enzymes and its traffic was sensitive to brefeldin A. However, trafficking of alpha-TIP was insensitive to brefeldin A treatment and was not affected by the ..
- Characterization of a novel Schizosaccharomyces pombe multidrug resistance transporter conferring brefeldin A resistanceT G Turi
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
Biochem Biophys Res Commun 213:410-8. 1995b>Brefeldin A disrupts protein secretion and causes the redistribution of the Golgi complex to endoplasmic reticulum in both mammalian cells and wild type Schizosaccharomyces pombe...
- Salicylic acid stimulates secretion of the normally symplastic enzyme mannitol dehydrogenase: a possible defense against mannitol-secreting fungal pathogensFang yi Cheng
Department of Horticultural Science, North Carolina State University, Raleigh, NC 27695 7609, USA
Planta 230:1093-103. 2009..After SA treatment, MTD was not detected in the Golgi apparatus, and its SA-induced secretion was resistant to brefeldin A, an inhibitor of Golgi-mediated protein transport...
- Inclusion of Brefeldin A during dendritic cell isolation allows in vitro detection of cross-presented self-antigensDirk Benke
Department of Cardiology, University clinic of the Rheinisch Westfälische Technische Hochschule, Aachen, Germany
J Immunol Methods 310:12-9. 2006..Here, we demonstrate that inclusion of Brefeldin A (BfA), an agent reported to block MHC class I-peptide complex turnover on the cell surface, during DC isolation ..
- Plasma membrane residence of hyaluronan synthase is coupled to its enzymatic activityKirsi Rilla
Department of Anatomy, University of Kuopio, FIN 70211 Kuopio, Finland
J Biol Chem 280:31890-7. 2005..time on the plasma membrane was less than 2 h in experiments with a relief and introduction, respectively, of brefeldin A. Constructs of HAS3 with 16- and 45-amino-acid C-terminal deletions mostly stayed within the ER, whereas a ..
- Interstitial adenosine triphosphate modulates muscle afferent nerve-mediated pressor reflexJianhua Li
Penn State Heart and Vascular Institute, Milton S Hershey Medical Center, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
Muscle Nerve 38:972-7. 2008..05 vs. control). In contrast, glibenclamide (an inhibitor of the ATP-binding cassette protein), monensin, and brefeldin A, which interfere with vesicular formation (or trafficking) and inhibit exocytosis, did not significantly affect ..
- Beta-COP localizes mainly to the cis-Golgi side in exocrine pancreasA Oprins
Department of Cell Biology, Medical School, Utrecht University, The Netherlands
J Cell Biol 121:49-59. 1993..After 10 min of Brefeldin A treatment 91% of beta-COP was cytoplasmic and only 7% associated with membranes of the Golgi complex...
- Regulation of brefeldin A-induced ER stress and apoptosis by mitochondrial NADP⁺-dependent isocitrate dehydrogenaseJung Lee Moon
School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Taegu 702 701, Republic of Korea
Biochem Biophys Res Commun 417:760-4. 2012b>Brefeldin A (BFA), an endoplasmic reticulum (ER)-Golgi transport inhibitor, has been shown to cause accumulation of proteins in the ER, ER stress, and ultimately apoptosis...
- On the action of Brefeldin A on Sec7-stimulated membrane-recruitment and GDP/GTP exchange of Arf proteinsJ Cherfils
Laboratoire d Enzymologie et Biochimie Structurales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France
Biochem Soc Trans 33:635-8. 2005..The drug BFA (Brefeldin A) was used to trap the complex that initiates the exchange reaction, providing a structural basis for its ..
- Dissection of COPI and Arf1 dynamics in vivo and role in Golgi membrane transportJohn F Presley
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
Nature 417:187-93. 2002..This role for Arf1/coatomer might provide a model for investigating the behaviour of other coat protein systems within cells...
- Brefeldin A-induced neurotoxicity in cultured spinal cord neuronsSeiji Kikuchi
Department of Neurology, Hokkaido University School of Medicine, Sapporo, Hokkaido, Japan
J Neurosci Res 71:591-9. 2003b>Brefeldin A (BFA) is a fungus metabolite that is known to cause the disassembly of the Golgi complex and apoptosis in exposed cells, both of which have been suggested as playing roles in the pathogenesis of neurodegenerative diseases, ..
- Brefeldin a inhibits circadian remodeling of chloroplast structure in the dinoflagellate gonyaulaxNasha Nassoury
Institut de recherche en biologie végétale, Departement de sciences biologiques, Universite de Montreal, 4101 Sherbrooke est, Montreal, Quebec, Canada H1X2B2
Traffic 6:548-61. 2005..Rubisco transit through the Golgi in dinoflagellates; hence, we blocked protein import into the plastids using Brefeldin A (BFA) to explore the mechanism for plastid remodeling...
- Quantitative ER <--> Golgi transport kinetics and protein separation upon Golgi exit revealed by vesicular integral membrane protein 36 dynamics in live cellsT Dahm
Light Microscopy Group, European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
Mol Biol Cell 12:1481-98. 2001....
- Structural integrity of the Golgi stack is essential for normal secretory functions of rat parotid acinar cells: effects of brefeldin A and okadaic acidHideaki Tamaki
Department of Anatomy, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan
J Histochem Cytochem 50:1611-23. 2002We examined the effects of specific inhibitors, brefeldin A (BFA) and okadaic acid (OA), on the ultrastructural organization of the Golgi apparatus and distributions of amylase, Golgi-associated proteins, and cathepsin D in the rat ..
- Inhibition of Japanese encephalitis virus maturation and transport in PS cells to cell surface by brefeldin AV J Lad
National Institute of Virology, Indian Council of Medical Research, 20 A, Dr Ambedkar Road, P B No 11, Pune 411 001, India
Acta Virol 46:187-90. 2002A brefeldin A (BFA) treatment of porcine stable kidney (PS) cells resulted in inhibition of Japanese encephalitis virus (JEV) maturation and its transport to the cell surface...
- Brefeldin A and filipin distinguish two globotriaosyl ceramide/verotoxin-1 intracellular trafficking pathways involved in Vero cell cytotoxicityAye Aye Khine
Department of Laboratory Medicine and Pathobiology, University of Toronto, 555 University Avenue, Toronto, Ontario, Canada
Glycobiology 14:701-12. 2004..b>Brefeldin A (BFA), which prevents Golgi-dependent retrograde traffic, protects cells from low VT1 concentrations but not ..
- Brefeldin A, but not monensin, enables flow cytometric detection of interleukin-4 within peripheral T cells responding to ex vivo stimulation with Chlamydia trachomatisRodolfo D Vicetti Miguel
Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15224, USA
J Immunol Methods 384:191-5. 2012..trachomatis genital tract infection were used to compare the ability of brefeldin A (BFA) and monensin (MN) to concurrently trap interferon-γ (IFN-γ), tumor necrosis factor (TNF), interleukin (..
- Reevaluation of the effects of brefeldin A on plant cells using tobacco Bright Yellow 2 cells expressing Golgi-targeted green fluorescent protein and COPI antiseraChristophe Ritzenthaler
Institut de Biologie Moleculaire des Plantes, 67084 Strasbourg Cedex, France
Plant Cell 14:237-61. 2002b>Brefeldin A (BFA) causes a block in the secretory system of eukaryotic cells by inhibiting vesicle formation at the Golgi apparatus...
- Multidrug resistance phenotype conferred by overexpressing bfr2+/pad1+/sks1+ or pap1+ genes and mediated by bfr1+ gene product, a structural and functional homologue of P-glycoprotein in Schizosaccharomyces pombeM Arioka
Department of Biotechnology, University of Tokyo, Japan
Biosci Biotechnol Biochem 62:390-2. 1998..These findings suggest that bfr1+ acts as the most downstream effector of the multidrug resistance conferred by bfr2+ and pap1+ genes...
- Cytohesin-1, a cytosolic guanine nucleotide-exchange protein for ADP-ribosylation factorE Meacci
Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1590, USA
Proc Natl Acad Sci U S A 94:1745-8. 1997..Cytohesin-1 also increased ARF1 binding to a Golgi fraction, but its effect was not inhibited by brefeldin A (BFA), a drug that reversibly inhibits Golgi function...
- Brefeldin A inhibits vesicular MHC class I processing in resting but not in CpG- and disruption-activated DCLiying Chen
Microbiology, Tumor and Cell Biology MTC, Karolinska Institute, 171 77 Stockholm, Sweden
Mol Immunol 46:158-65. 2008..Here we investigate the sensitivity of this VP to Brefeldin A (BFA), a fungal metabolite which inhibits vesicular trafficking by preventing the activation of some ADP-..
- Brefeldin A influences the cell surface abundance and intracellular pools of low and high ouabain affinity Na+, K(+)-ATPase alpha subunit isoforms in articular chondrocytesA Mobasheri
Department of Biomedical Sciences, School of Biosciences, University of Westminster, London, United Kingdom
Histol Histopathol 14:427-38. 1999..05) upregulation of Na+, K(+)-ATPase density and treatment with various pharmacological inhibitors (Brefeldin A, monensin and cycloheximide) significantly (p < 0.001) blocked the upregulation...
- Plasmodium vivax and Plasmodium chabaudi: intraerythrocytic traffic of antigenically homologous proteins involves a brefeldin A-sensitive secretory pathwayC Bracho
Instituto Venezolano de Investigaciones Cientificas IVIC, Caracas, Venezula
Eur J Cell Biol 80:164-70. 2001..In the presence of brefeldin A (BFA), secretion of antigenically homologous Pc-190 into the host cell cytoplasm was inhibited and the antigen ..
- The role of de novo ceramide synthesis in the mechanism of action of the tricyclic xanthate D609Ryan J Perry
Department of Pediatrics, Atlantic Research Centre, Dalhousie University, Halifax, Nova Scotia, Canada
J Lipid Res 45:164-73. 2004..In D609-treated CHO-K1 cells, sphingomyelin synthesis was stimulated by brefeldin A, and C5-DMB-ceramide transport to the Golgi apparatus was blocked, indicating ceramide accumulation in the ..
- Brefeldin A enhances Helicobacter pylori vacuolating cytotoxin-induced vacuolation of epithelial cellsRichard H Argent
Institute of Infection, Immunity and Inflammation, and the Wolfson Digestive Diseases Centre, University of Nottingham, Queen s Medical Centre, Nottingham, NG7 2UH, UK
FEMS Microbiol Lett 237:163-70. 2004..We show that the Golgi-disturbing agent brefeldin A (BFA) enhances VacA-induced vacuolation of epithelial cells by Helicobacter pylori co-culture and, importantly, ..
- Brefeldin A disrupts asymmetric spindle positioning in mouse oocytesLing Wang
Ottawa Health Research Institute, Ottawa Hospital, Civic Campus, 1053 Carling Avenue, Ottawa, Canada
Dev Biol 313:155-66. 2008..We show here that treating denuded mouse oocytes with brefeldin A, an inhibitor of Golgi-based membrane fusion, abolished the asymmetric positioning of the metaphase I spindle ..
- Low-level doxorubicin resistance in P-glycoprotein-negative human pancreatic tumour PSN1/ADR cells implicates a brefeldin A-sensitive mechanism of drug extrusionV N Verovski
Cancer Research Unit, Department of Radiotherapy, Academic Hospital, Free University Brussels, Belgium
Br J Cancer 73:596-602. 1996..In addition, these defects were reversible by the vesicular traffic inhibitors brefeldin A, fluoroaluminate and nocodazole...
- ER stress: role in myocyte apoptosis and myocardial remodelingKrishna Singh; Fiscal Year: 2013..Well-known pharmacological ER stressors (brefeldin A, thapsigargin and tunicamycin) activated GSK-32 and increased cardiac myocyte apoptosis...
- Mechanism of Apoptosis and Inhibition of Tumor Progression and Metastasis by Par-Vivek M Rangnekar; Fiscal Year: 2012..The apoptotic activity was diminished in the CM from Par-4 transfectants treated with brefeldin A, indicating Par-4 and SAC secretion (or sPar-4 and sSAC, respectively) occurs by the classical pathway (i.e...
- Enteropathogenic E. coli effector proteins in intestinal tight junction recoveryLILA GOLLOGLY; Fiscal Year: 2013..IEC will be transfected to express NleA, or treated with brefeldin A, and the effects on TJ recovery following calcium chelation will be measured...
- A Small Molecule Approach Toward Understanding Bacterial Toxin TransportJOSE BERNARDO SAENZ; Fiscal Year: 2010..One novel compound, B06, produces a brefeldin A-like dispersal of the Golgi, and preliminary evidence suggests that it could serve as a useful tool in ..
- Pathogenesis of Lyme Borreliosis in the Rhesus MonkeyMARIO PHILIPP; Fiscal Year: 2007..be stimulated ex vivo with lipoprotein or live spirochetes in the presence of the protein-secretion blocker Brefeldin A. After fixation and cutting of frozen sections, the cytokine/mediator-producing cell phenotype will be ..
- FUNGAL POLYKETIDE SYNTHASESCHARLES SIH; Fiscal Year: 2001..of the fungal PKSs involved in the biosynthesis of the biologically active compounds mevinolin (lovastatin) and brefeldin A. We will strive to establish how these two multifunctional proteins, which are likely to contain sets of ..
- ABNORMAL TERMINAL COLON OF THE 1S/1S MUTANT MOUSETAUBE ROTHMAN; Fiscal Year: 2000..They will use immunocytochemistry to identify these cells. They also will use a drug called brefeldin A which blocks secretion of proteins and should enhance the identification of the cells...
- ARF PROTEINS IN CELLULAR REGULATION OF PHOSPHOLIPASE DGuillermo Romero; Fiscal Year: 2000..Cells that overexpress mutated receptors do not show this behavior. Likewise, the isoprenoid Brefeldin A, an agent that disrupts intracellular membrane traffic and whose effects are linked to the inhibition of ARF ..
- PROCESSING OF PROINSULIN/INSULIN BY B-CELL ORGANELLESPhilippe Halban; Fiscal Year: 1993..b>Brefeldin A inhibits RER-Golgi transfer but allows the reverse movement of molecules (regurgitation from the Golgi)...
- Engineering Biosynthesis of Brefeldin A and Its AnalogsWeiwen Zhang; Fiscal Year: 2002..b>Brefeldin A, a macrolide polyketide produced by Eupenicilium brefeldianum, exhibits a wide range of antibiotic, antifungal ..
- ANTIVIRAL/BIOACTIVE COMPOUNDS--SYNTHESIS AND CHEMISTRYELIAS COREY; Fiscal Year: 1999..agent, pseudopterosin E; (7) synthesis of the inhibitor of protein translocation and processing, brefeldin A; (8) synthesis of the inhibitors of biosynthesis of initiation factor 4D; (9) synthesis of new serotonin uptake ..
- CELLULAR BIOPHYSICSEric Siggia; Fiscal Year: 2000..The kinetics of the adsorption of the Golgi by the ER induced by Brefeldin A treatment are not diffusive and a model involving surface tension driven flow is being pursued.
- STUDIES OF SYNAPSE FORMATIONLYNN LANDMESSER; Fiscal Year: 2007..designed to define the role of the 140 and/or 120 kD isoform of NCAM in the down regulation of the immature, brefeldin A and L-type Ca+2 channel sensitive, immature vesicle cycling/release mechanism from the axon...
- Pertussis Toxin Trafficking and Processing in CellsNICHOLAS CARBONETTI; Fiscal Year: 2005..Therefore the specific aims of this proposal are to investigate the trafficking, processing and transcytosis of PT in mammalian cells and the key features of this toxin that mediate these events. ..
- Role of Pertussis Toxin in Bordetella pertussis infectionNicholas H Carbonetti; Fiscal Year: 2010..pertussis infection will increase our understanding of the pathogenic mechanisms of this organism, and may lead to novel therapeutic approaches to combat pertussis infection and disease. ..
- Synthesis of Macrolides, Steroids, Cyclopentanoids, etc.BARRY TROST; Fiscal Year: 2009..These new synthetic methods apply to many structural types beyond those illustrated and constitutes a significant to gain access to complex molecular targets more easily. ..
- Effect of Pertussis Toxin on Cough in Guinea PigNICHOLAS CARBONETTI; Fiscal Year: 2005..abstract_text> ..
- LACTOSE SYNTHESIS REGULATION: ROLE OF GLUCOSE TRANSPORTPETER HANEY; Fiscal Year: 2002..These will be related to changes in glucose transport activity an lactose synthesis that are essential for milk production. ..
- Engineering a CFTR Deficient Porcine Model of CFSCOTT O GRADY; Fiscal Year: 2007..abstract_text> ..
- Genetics of SV40 Entry and Minichromosome TransportLEONARD NORKIN; Fiscal Year: 2008..Recent studies report evidence that SV40 infection may lead to neoplastic disease in humans, including mesotheliornas, osteosarcomas, childhood brain tumors, and non-Hodgkin's Iymphoma. ..
- Dysfuntional antigen presenting cell responses to influenza in geriatric individuDAVID CANADAY; Fiscal Year: 2008..Ag processing and presentation efficiency of B cells is important for them to receive optimal costimulation from CD4+ T helper cells. [unreadable] [unreadable] [unreadable]..
- Estrogen and Aging effects on Transvaginal TransportGeorge Gorodeski; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- RHESUS MACAQUE MODEL OF TUBERCULOSISDAVID LEWINSOHN; Fiscal Year: 2001..g., CT scans, quantitative culture of mycobacteria from BAL fluids and liver biopsies) which correlate with survival. ..
- CD8+T cell responses to Chlamydia pneumoniaeBenjamin Wizel; Fiscal Year: 2006..pneumoniae and will suggest methods of immunological intervention in infections caused by C. pneumoniae and other chlamydial pathogens. ..
- Mechanisms of MHC-II antigen processing of HIV in macrophages and dendritic cellsDAVID CANADAY; Fiscal Year: 2008..unreadable] [unreadable] [unreadable] [unreadable]..
- IRON BINDING PROTEINS AND CONTROL OF IRON METABOLISMPhilip Aisen; Fiscal Year: 2001..Understanding the fundamental chemistry of iron transport, storage and utilization will provide the rational basis for managing the multitude of diseases where aberrations of iron metabolism contribute to cellular injury and death. ..
- Connexin acetylation: Impact on gap junction function and HDAC inhibitor responsJEANNETTE CHLOE BULINSKI; Fiscal Year: 2010..In a broader view, we will also demonstrate novel roles by which protein acetylation regulates cellular functions not directly involved with gene expression. ..
- Functional Characterization of Nuclear Myosin I in TranscriptionPrimal de Lanerolle; Fiscal Year: 2010..The experiments described in this proposal investigate the role of a new protein in transcription. They have the potential to increase our understanding of the transcription process at the molecular level ..
- CFTR-Ion Conduction and Gating MechanismsDavid C Dawson; Fiscal Year: 2010....
- The Role of Arf GTPases in Endocytosis and Postendocytic TransportJames E Casanova; Fiscal Year: 2010....
- A new approach to xenotransplantation in primateJuan Contreras; Fiscal Year: 2005..The research proposed in this grant will provide significant information to the xenotransplant research and the potential use of porcine islets as an alternative to human tissue for pancreatic islet transplantation. ..
- Intercellular signaling during terminal differentiationWilliam Loomis; Fiscal Year: 2009..Together these studies will add to the foundation for improvements in the diagnosis and treatment of neurological diseases. ..
- Analysis of apoptotic signaling induced by the mammalian endoplasmic reticulumJAY BREWSTER; Fiscal Year: 2008..of ER stress and its signaling mechanisms will offer vital insight into the cell biology of stressed cells, and will be valuable to our understanding of diseases that result from abnormal ER function [unreadable] [unreadable] [unreadable]..
- Mitogen activated protein kinases regulation-PTH/PTHrPHesham Tawfeek; Fiscal Year: 2008..Primary osteoblastic cell culture from wild type and genetically engineered mice as well as several other biochemical and pharmacological tools will be employed to achieve the goals of the study. ..
- Hormonal Therapy to Increase Islet Recovery/Engraftment From Multiorgan DonorsJuan Contreras; Fiscal Year: 2008..abstract_text> ..
- Effects of brain-death on islet recovery and functional*John Thompson; Fiscal Year: 2006..Overall, these studies will bring novel information about human islet biology and will provide c potential therapeutic strategy to enhance the therapeutic efficacy of PIT as a cure for diabetes. ..
- Antigen Processing in HIV-infected IndividualsDAVID CANADAY; Fiscal Year: 2006..e. HIV-induced deficits in both APC and T cells were potentially present), our studies will provide the first well-controlled comparison of APC function in HIV+ and HIV- persons. ..
- Strategy for islet recovery, functionality & engraftmentJuan Contreras; Fiscal Year: 2006..The proposed studies are expected to yield novel information about the effects of BD on isolated islet biology and to develop potential targets for therapeutic intervention, thereby enhancing the efficacy of PIT as a cure for diabetes. ..
- Cysteine String Protein and Acute PancreatitisGUY GROBLEWSKI; Fiscal Year: 2004..These studies will allow us to directly evaluate the role of CSP in the secretory pathway of acini under physiological and pathophysiological conditions. ..
- ANTIBODY PROBES OF CNS MITOCHONDRIAL APOPTOTIC FUNCTIONRonald Jemmerson; Fiscal Year: 2004..The feasibility of the proposed research is supported by preliminary results and by the participation of on-site collaborators with expertise in the field of neuroscience, in particular the physiology of CNS mitochondria. ..
- New Perspectives in Transporter Biology-FASEB conferenceRobert Edwards; Fiscal Year: 2003..Each oral session will be followed by a panel discussion of the topic. To promote the advancement of junior faculty, the most exciting poster submissions will be selected for short oral presentations during the regular oral sessions. ..
- CD8+ T CELLS AND MYCOPLASMA TUBERCULOSISDAVID CANADAY; Fiscal Year: 2003..Aim 3. To characterize the functional CD8+ T cell responses to specific proteins and peptides from in patients with active M. tuberculosis infection. ..
- NUCLEAR MYOSIN IPrimal de Lanerolle; Fiscal Year: 2003..This proposal will also develop a number of tools (eg: plasmids, monoclonal and anti-peptide antibodies, proteins expressed in baculovirus, knock-out cells) that will be instrumental in realizing our long range goal. ..
- MOLECULAR NETWORKS THAT COORDINATE DIFFERENTIATIONWilliam Loomis; Fiscal Year: 2003..Together these studies will help to establish a signal transduction pathway that can serve as a model for studies in other systems. ..
- IRON TRANSPORT IN MAMMALIAN CELLSPhilip Aisen; Fiscal Year: 2003..Project 5: Structure, function and dynamics in transferrin investigates the molecular mechanisms through which the uptake and release of iron and other metals by transferrin are modulated by physiological factors. ..
- ASSEMBLY AND TARGETING OF EPITHELIAL P-TYPE ATPASESCurtis Okamoto; Fiscal Year: 2001....
- MYOSIN LIGHT CHAIN KINASE IN SMOOTH MUSCLE PROLIFERATIONPrimal de Lanerolle; Fiscal Year: 2002..This, in turn could result in improved therapy for intimal hyperplasia. ..
- NOVEL USE OF ZEBRAFISH FOR APOPTOSIS ASSAYChuenlei Parng; Fiscal Year: 2004..Complementary visual and flow cytometry based assays will also be developed to provide comprehensive analysis of apoptosis in zebrafish. ..
- GTPase Regulation of Smooth Muscle ContractionPrimal de Lanerolle; Fiscal Year: 2004..These experiments represent a powerful test of our hypothesis and they will provide important insights into the molecular mechanisms that regulate the calcium sensitivity of smooth muscle contraction. ..
- TRANSPORT OF NEUROTRANSMITTERS INTO SYNAPTIC VESICLESRobert Edwards; Fiscal Year: 2005..Third, we will manipulate the expression, localization and regulation of the transporters in vivo to assess the role of these mechanisms in synaptic transmission and behavior. ..
- Nitric oxide in bladder neural-epithelial signalingLori Birder; Fiscal Year: 2005..Elucidation of mechanisms impacting on urothelial function may provide insights into the pathology of bladder dysfunction. ..
- Role of the GTPase ARF6 in Epithelial Cell MigrationJames Casanova; Fiscal Year: 2005..abstract_text> ..
- TRANSEPITHELIAL TRANSPORT OF IMMUNOGLOBULINSJames Casanova; Fiscal Year: 2002..These studies will provide insight into the mechanisms by which macromolecules are transported across epithelia, a process that is particularly important in the mucosal immunity. ..
- Proteomic Biomarkers of Health BehaviorsPaul Lampe; Fiscal Year: 2005..abstract_text> ..
- Zebrafish Screen for Neuroprotective Anti-oxidantsChuenlei Parng; Fiscal Year: 2005..Assessment of drug effects in a convenient vertebrate model, prior to evaluation in a complex system, such as mouse, can potentially streamline the drug development process and dramatically reduce costs. ..
- Functional Genomics of Dictyostelium DevelopmentWilliam Loomis; Fiscal Year: 2005..Predictions derived from such networks will be tested when microarray analyses are extended to include further developmental mutants. ..
- Cardiotoxicity Drug Screening Using ZebrafishChuenlei Parng; Fiscal Year: 2004..Zebrafish drug screens can serve as an intermediate step between cell-based and mammalian testing, streamlining the drug development timeline. ..
- ASSAY FOR DETERMINING DRUG TOXICITYChuenlei Parng; Fiscal Year: 2004..abstract_text> ..
- REGULATION OF EXTRACELLULAR SUPEROXIDE DISMUTASE BY PROTRussell Bowler; Fiscal Year: 2004..This will not only define new aspects of the pathogenesis and regulation of vascular tone and inflammation, but can open new approaches to pharmacologically control these events. ..
- MICROTUBULE/KERATIN INTERACTIONS DURING SPERMATOGENESISABRAHAM KIERSZENBAUM; Fiscal Year: 2002..Engineering mouse models in which the expression of K9 is null, is important for both reproductive biology and cancer research. ..