diacetyl

Summary

Summary: Carrier of aroma of butter, vinegar, coffee, and other foods.

Top Publications

  1. ncbi Aluminium causes variable responses in actin filament cytoskeleton of the root tip cells of Triticum turgidum
    G Frantzios
    Department of Botany, Faculty of Biology, University of Athens, Athens, Greece
    Protoplasma 225:129-40. 2005
  2. pmc Cytosolic localization of acetohydroxyacid synthase Ilv2 and its impact on diacetyl formation during beer fermentation
    Suvarna Dasari
    Institut für Lebensmittelwissenschaft und Biotechnologie, Fg Gärungstechnologie 150f, Universitat Hohenheim, Garbenstr 23, D 70599 Stuttgart, Germany
    Appl Environ Microbiol 77:727-31. 2011
  3. ncbi Biocatalytic production of (2S,3S)-2,3-butanediol from diacetyl using whole cells of engineered Escherichia coli
    Lixiang Li
    State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China
    Bioresour Technol 115:111-6. 2012
  4. ncbi Production of S-acetoin from diacetyl by Escherichia coli transformant cells that express the diacetyl reductase gene of Paenibacillus polymyxa ZJ-9
    J Gao
    Schol of Chemical and Biological Engineering, Yancheng Institute of Technology, Yancheng, China
    Lett Appl Microbiol 57:274-81. 2013
  5. ncbi Production of (3S)-acetoin from diacetyl by using stereoselective NADPH-dependent carbonyl reductase and glucose dehydrogenase
    Chao Gao
    State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China
    Bioresour Technol 137:111-5. 2013
  6. ncbi Determination of organic acids, sugars, diacetyl, and acetoin in cheese by high-performance liquid chromatography
    G Zeppa
    Dipartimento Valorizzazione e Protezione Risorse Agroforestali Settore Microbiologia e Industrie agrarie, Universita di Torino, Via L da Vinci 44, 10095, Grugliasco, Torino, Italy
    J Agric Food Chem 49:2722-6. 2001
  7. pmc Genetic manipulation of the pathway for diacetyl metabolism in Lactococcus lactis
    S R Swindell
    Institute of Food Research, Colney, United Kingdom
    Appl Environ Microbiol 62:2641-3. 1996
  8. ncbi Identification of Sc-type ILV6 as a target to reduce diacetyl formation in lager brewers' yeast
    C T Duong
    Department of Microbiology and Genetics, Berlin University of Technology, Seestr 13, D 13353 Berlin, Germany
    Metab Eng 13:638-47. 2011
  9. pmc Separation of propulsive and adhesive traction stresses in locomoting keratocytes
    T Oliver
    Department of Mechanical Engineering and Materials Science, Duke University, Durham, North Carolina 27708 0300, USA
    J Cell Biol 145:589-604. 1999
  10. pmc Activation of the diacetyl/acetoin pathway in Lactococcus lactis subsp. lactis bv. diacetylactis CRL264 by acidic growth
    Nieves García-Quintáns
    Departamento de Ciencia de Proteínas, Centro de Investigaciones Biologicas, Ramiro de Maeztu 9, 28040 Madrid, Spain
    Appl Environ Microbiol 74:1988-96. 2008

Detail Information

Publications226 found, 100 shown here

  1. ncbi Aluminium causes variable responses in actin filament cytoskeleton of the root tip cells of Triticum turgidum
    G Frantzios
    Department of Botany, Faculty of Biology, University of Athens, Athens, Greece
    Protoplasma 225:129-40. 2005
    ..These results favour the hypothesis that the actomyosin system in the Al-treated cells, among other roles, participates in the mechanism controlling callose deposition...
  2. pmc Cytosolic localization of acetohydroxyacid synthase Ilv2 and its impact on diacetyl formation during beer fermentation
    Suvarna Dasari
    Institut für Lebensmittelwissenschaft und Biotechnologie, Fg Gärungstechnologie 150f, Universitat Hohenheim, Garbenstr 23, D 70599 Stuttgart, Germany
    Appl Environ Microbiol 77:727-31. 2011
    b>Diacetyl (2,3-butanedione) imparts an unpleasant "butterscotch-like" flavor to alcoholic beverages such as beer, and therefore its concentration needs to be reduced below the sensory threshold before packaging...
  3. ncbi Biocatalytic production of (2S,3S)-2,3-butanediol from diacetyl using whole cells of engineered Escherichia coli
    Lixiang Li
    State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China
    Bioresour Technol 115:111-6. 2012
    ..Whole cells of the recombinant E. coli was used to produce (2S,3S)-2,3-BD from diacetyl. Under optimal conditions, high-optical-purity (2S,3S)-2,3-BD (purity >99%) was obtained with concentrations ..
  4. ncbi Production of S-acetoin from diacetyl by Escherichia coli transformant cells that express the diacetyl reductase gene of Paenibacillus polymyxa ZJ-9
    J Gao
    Schol of Chemical and Biological Engineering, Yancheng Institute of Technology, Yancheng, China
    Lett Appl Microbiol 57:274-81. 2013
    ..In the current work, a gene encoding diacetyl reductase (DAR) from a Paenibacillus polymyxa strain ZJ-9 was cloned and expressed in Escherichia coli...
  5. ncbi Production of (3S)-acetoin from diacetyl by using stereoselective NADPH-dependent carbonyl reductase and glucose dehydrogenase
    Chao Gao
    State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China
    Bioresour Technol 137:111-5. 2013
    ..carbonyl reductase (Gox0644) from Gluconobacter oxydans DSM 2003 was confirmed to have a good ability to reduce diacetyl (DA) to produce (3S)-AC. In this work, the NADPH-dependent carbonyl reductase was expressed and purified...
  6. ncbi Determination of organic acids, sugars, diacetyl, and acetoin in cheese by high-performance liquid chromatography
    G Zeppa
    Dipartimento Valorizzazione e Protezione Risorse Agroforestali Settore Microbiologia e Industrie agrarie, Universita di Torino, Via L da Vinci 44, 10095, Grugliasco, Torino, Italy
    J Agric Food Chem 49:2722-6. 2001
    ..and hippuric), some free fatty acids (formic, acetic, propionic, butyric, isobutyric, valeric, and isovaleric), diacetyl, and acetoin were separated on an Aminex HPX-87H column using a simple isocratic HPLC method and identified by ..
  7. pmc Genetic manipulation of the pathway for diacetyl metabolism in Lactococcus lactis
    S R Swindell
    Institute of Food Research, Colney, United Kingdom
    Appl Environ Microbiol 62:2641-3. 1996
    b>Diacetyl is an important food flavor compound produced by certain strains of citrate-metabolizing lactic acid bacteria. Citrate is converted to pyruvate, from which diacetyl is produced via intermediate alpha-acetolactate...
  8. ncbi Identification of Sc-type ILV6 as a target to reduce diacetyl formation in lager brewers' yeast
    C T Duong
    Department of Microbiology and Genetics, Berlin University of Technology, Seestr 13, D 13353 Berlin, Germany
    Metab Eng 13:638-47. 2011
    b>Diacetyl causes an unwanted buttery off-flavor in lager beer. It is spontaneously generated from α-acetolactate, an intermediate of yeast's valine biosynthesis released during the main beer fermentation...
  9. pmc Separation of propulsive and adhesive traction stresses in locomoting keratocytes
    T Oliver
    Department of Mechanical Engineering and Materials Science, Duke University, Durham, North Carolina 27708 0300, USA
    J Cell Biol 145:589-604. 1999
    ....
  10. pmc Activation of the diacetyl/acetoin pathway in Lactococcus lactis subsp. lactis bv. diacetylactis CRL264 by acidic growth
    Nieves García-Quintáns
    Departamento de Ciencia de Proteínas, Centro de Investigaciones Biologicas, Ramiro de Maeztu 9, 28040 Madrid, Spain
    Appl Environ Microbiol 74:1988-96. 2008
    ..a fermentative microorganism, which cometabolizes glucose and citrate to yield aroma compounds through the diacetyl/acetoin biosynthetic pathway. Our previous results have shown that under acidic growth Lactococcus bv...
  11. pmc Lactococcus lactis as a cell factory for high-level diacetyl production
    J Hugenholtz
    Wageningen Centre for Food Sciences and NIZO Food Research, 6710 BA Ede, The Netherlands
    Appl Environ Microbiol 66:4112-4. 2000
    We report the engineering of Lactococcus lactis for the efficient conversion of sugar into diacetyl by combining NADH-oxidase overproduction and alpha-acetolactate decarboxylase inactivation...
  12. ncbi The 'buttery' attribute of wine--diacetyl--desirability, spoilage and beyond
    Eveline J Bartowsky
    The Australian Wine Research Institute, PO Box 197, Glen Osmond, SA 5064, Australia
    Int J Food Microbiol 96:235-52. 2004
    The diketone, diacetyl, is a major flavour metabolite produced by lactic acid bacteria (LAB)...
  13. pmc Diacetyl and alpha-acetolactate overproduction by Lactococcus lactis subsp. lactis biovar diacetylactis mutants that are deficient in alpha-acetolactate decarboxylase and have a low lactate dehydrogenase activity
    C Monnet
    Laboratoire de Génie et Microbiologie des Procédés Alimentaires, Institut National de la Recherche Agronomique, 78850 Thiverval Grignon, France
    Appl Environ Microbiol 66:5518-20. 2000
    ..biovar diacetylactis strains are utilized in several industrial processes for producing the flavoring compound diacetyl or its precursor alpha-acetolactate...
  14. ncbi Ca2+-independent alterations in diastolic sarcomere length and relaxation kinetics in a mouse model of lipotoxic diabetic cardiomyopathy
    Thomas P Flagg
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    Circ Res 104:95-103. 2009
    ....
  15. ncbi Production of L-2,3-butanediol by a new pathway constructed in Escherichia coli
    S Ui
    Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Kofu, Yamanashi, Japan
    Lett Appl Microbiol 39:533-7. 2004
    ..To rectify this situation, we attempted to produce L-BD from diacetyl (DA) by producing simultaneous expression of diacetyl reductase (DAR) and L-2,3-butanediol dehydrogenase (BDH) ..
  16. ncbi Diacetyl and acetoin production from whey permeate using engineered Lactobacillus casei
    Inmaculada Nadal
    Laboratorio de Bacterias Lacticas y Probioticos, Instituto de Agroquimica y Tecnologia de Alimentos CSIC, 46100 Burjassot, Valencia, Spain
    J Ind Microbiol Biotechnol 36:1233-7. 2009
    The capability of Lactobacillus casei to produce the flavor-related compounds diacetyl and acetoin from whey permeate has been examined by a metabolic engineering approach. An L...
  17. ncbi Effects of mechanical uncouplers, diacetyl monoxime, and cytochalasin-D on the electrophysiology of perfused mouse hearts
    Linda C Baker
    Dept of Cell Biology and Physiology, S314 Biomedical Science Tower, University of Pittsburgh School of Medicine, 3500 Terrace Street, Pittsburgh, PA 15261, USA
    Am J Physiol Heart Circ Physiol 287:H1771-9. 2004
    Chemical uncouplers diacetyl monoxime (DAM) and cytochalasin D (cyto-D) are used to abolish cardiac contractions in optical studies, yet alter intracellular Ca(2+) concentration ([Ca(2+)](i)) handling and vulnerability to arrhythmias in a ..
  18. ncbi Induction of chemotaxis to sodium chloride and diacetyl and thermotaxis defects by microcystin-LR exposure in nematode Caenorhabditis elegans
    Yunhui Li
    Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Nanjing 210009, China
    J Environ Sci (China) 21:971-9. 2009
    ..In the present study, we provided evidence to indicate the neurotoxicity on chemotaxis to NaCl and diacetyl, and thermotaxis from MC-LR exposure to C. elegans...
  19. ncbi odr-10 encodes a seven transmembrane domain olfactory receptor required for responses to the odorant diacetyl
    P Sengupta
    Howard Hughes Medical Institute, Programs in Developmental Biology, Neuroscience, and Genetics, University of California, San Francisco, 94143 0452, USA
    Cell 84:899-909. 1996
    ..We show that the C. elegans odr-10 gene is likely to encode a receptor for the odorant diacetyl. odr-10 mutants have a specific defect in chemotaxis to diacetyl, one of several odorants detected by the AWA ..
  20. pmc Fine tuning of the lactate and diacetyl production through promoter engineering in Lactococcus lactis
    Tingting Guo
    State Key Laboratory of Microbial Technology, Shandong University, Jinan, People s Republic of China
    PLoS ONE 7:e36296. 2012
    ..For fine tuning of the distribution of glycolytic flux at the pyruvate branch from lactate to diacetyl and balancing the production of the two metabolites under aerobic conditions, a constitutive promoter library was ..
  21. pmc Engineering of cofactor regeneration enhances (2S,3S)-2,3-butanediol production from diacetyl
    Yu Wang
    1 State Key Laboratory of Microbial Metabolism, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People s Republic of China 2 State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People s Republic of China
    Sci Rep 3:2643. 2013
    ..Introduction of FDH resulted in higher (2S,3S)-2,3-BD concentration, productivity and yield from diacetyl, and large increase in the intracellular NADH concentration...
  22. ncbi New insights into Lactococcus lactis diacetyl- and acetoin-producing strains isolated from diverse origins
    Delphine Passerini
    Universite de Toulouse, Universite Paul Sabatier, 118 route de Narbonne, Toulouse, France
    Int J Food Microbiol 160:329-36. 2013
    ..lactis biovar diacetylactis strains are used in the dairy industry for generating acetoin and notably diacetyl which imparts a high level of buttery flavor notes...
  23. ncbi A proposal for a safe exposure level for diacetyl
    David S Egilman
    Never Again Consulting, Attleboro, MA, USA
    Int J Occup Environ Health 17:122-34. 2011
    b>Diacetyl is a naturally occurring compound that has been used in concentrated form as a food additive, particularly in butter flavorings...
  24. pmc Reaction of the butter flavorant diacetyl (2,3-butanedione) with N-α-acetylarginine: a model for epitope formation with pulmonary proteins in the etiology of obliterative bronchiolitis
    James M Mathews
    RTI International, P O Box 12194, Research Triangle Park, North Carolina 27709, United States
    J Agric Food Chem 58:12761-8. 2010
    The butter flavorant diacetyl (2,3-butanedione) is implicated in causing obliterative bronchiolitis in microwave popcorn plant workers...
  25. ncbi Blood and urinary levels of ethanol, acetaldehyde, and C4 compounds such as diacetyl, acetoin, and 2,3-butanediol in normal male students after ethanol ingestion
    M Otsuka
    Faculty of Pharmaceutical Sciences, Okayama University, Japan
    Alcohol 17:119-24. 1999
    ..The student with the inactive form of ALDH2 was flushed and his levels of 2,3-butanediol and acetaldehyde in blood and urine were found to be the highest...
  26. ncbi Construction of self-cloning bottom-fermenting yeast with low vicinal diketone production by the homo-integration of ILV5
    K Kusunoki
    Research Laboratories for Brewing, Asahi Breweries Ltd, Ibaraki, Japan
    Yeast 29:435-42. 2012
    The vicinal diketones (VDK), such as diacetyl and 2,3-pentandione, impart an unpleasant butter-like flavour to beer...
  27. ncbi Correcting diacetyl concentrations from air samples collected with NIOSH method 2557
    Jean Cox-Ganser
    Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, 1095 Willowdale Road, Morgantown, WV 26505 2888, USA
    J Occup Environ Hyg 8:59-70. 2011
    b>Diacetyl (2,3-butanedione), a diketone chemical used to impart a buttery taste in many flavoring mixtures, has been associated with bronchiolitis obliterans in several industrial settings...
  28. ncbi Method for the simultaneous assay of diacetyl and acetoin in the presence of alpha-acetolactate: application in determining the kinetic parameters for the decomposition of alpha-acetolactate
    Ken Kobayashi
    National Research Institute of Brewing, 3 7 1 Kagamiyama, Higashi Hiroshima, Hiroshima 739 0046, Japan
    J Biosci Bioeng 99:502-7. 2005
    A simultaneous assay method for diacetyl and acetoin was developed to investigate the formation of diacetyl during the brewing of alcoholic beverages...
  29. ncbi Influence of additives on the properties of Bis-GMA/Bis-GMA analog comonomers and corresponding copolymers
    Anuradha Prakki
    Department of Operative Dentistry, Endodontics and Dental Materials, University of Sao Paulo, Bauru, Sao Paulo, Brazil
    Dent Mater 23:1199-204. 2007
    ....
  30. pmc Inhalation dosimetry of diacetyl and butyric acid, two components of butter flavoring vapors
    John B Morris
    Toxicology Program, Department of Pharmaceutical Sciences, University of Connecticut, Storrs, Connecticut 06269, USA
    Toxicol Sci 108:173-83. 2009
    ..The goal of the current study was to characterize inhalation dosimetric patterns of diacetyl and butyric acid, two components of BFV, and to develop a hybrid computational fluid dynamic-physiologically ..
  31. ncbi Role of diacetyl metabolite in alcohol toxicity and addiction via electron transfer and oxidative stress
    Peter Kovacic
    Department of Chemistry, San Diego State University, San Diego, CA, USA
    Arch Toxicol 79:123-8. 2005
    ..Metabolism affords mainly acetic acid via acetaldehyde. A minor metabolite, diacetyl (an alpha-dicarbonyl), arises from the aldehyde...
  32. ncbi Developmental changes in chemotactic response and choice of two attractants, sodium acetate and diacetyl, in the nematode Caenorhabditis elegans
    Tetsuya Matsuura
    Department of Welfare Engineering, Faculty of Engineering, Iwate University, 4 3 5 Ueda Morioka 020 8551, Japan
    Comp Biochem Physiol A Mol Integr Physiol 147:920-7. 2007
    ..behavior of the nematode Caenorhabditis elegans to chemical attractants, water-soluble sodium acetate and odorant diacetyl, was investigated using nematodes at various developmental stages to examine the effects of postembryonic ..
  33. ncbi Formation of diacetyl and acetoin by Lactococcus lactis via aspartate catabolism
    D Le Bars
    Unité de Biochimie Bactérienne, UR477, INRA, Jouy en Josas, France
    J Appl Microbiol 104:171-7. 2008
    To verify whether diacetyl can be produced by Lactococcus lactis via amino acid catabolism, and to investigate the impact of the pH on the conversion.
  34. ncbi Use of diacetyl to reduce the load of Vibrio vulnificus in the Eastern oyster, Crassostrea virginica
    J M Birkenhauer
    Department of Biology, University of North Carolina at Charlotte, 9201 University City Boulevard, Charlotte, North Carolina 28223, USA
    J Food Prot 66:38-43. 2003
    ..conducted in our laboratory have examined various generally recognized as safe compounds and have determined that diacetyl, a component of butter, is among the most effective of these compounds in reducing loads of V...
  35. ncbi In vitro wear, surface roughness and hardness of propanal-containing and diacetyl-containing novel composites and copolymers based on bis-GMA analogs
    Anuradha Prakki
    Department of Operative Dentistry, Endodontics and Dental Materials, University of Sao Paulo, Bauru Dental School, Bauru, São Paulo 17043 170, Brazil
    Dent Mater 24:410-7. 2008
    ....
  36. ncbi Bronchiolitis obliterans syndrome in chemical workers producing diacetyl for food flavorings
    Frits G B G J van Rooy
    Netherlands Expertise Centre for Occupational Respiratory Disorders, Utrecht, The Netherlands
    Am J Respir Crit Care Med 176:498-504. 2007
    Workers in microwave popcorn plants are at risk of developing bronchiolitis obliterans associated with exposure to butter flavoring volatiles, including diacetyl.
  37. ncbi Localised depletion of polymerised actin at the front of Walker carcinosarcoma cells increases the speed of locomotion
    Hansuli Keller
    Institute of Pathology, University of Bern, Bern, Switzerland
    Cell Motil Cytoskeleton 53:189-202. 2002
    ..They are best compatible with a cortical contraction/frontal expansion model, but they are not compatible with models implying that actin polymerisation or actomyosin contraction at the front drive locomotion of the cells studied...
  38. ncbi Butanedione monoxime increases the viability and yield of adult cardiomyocytes in primary cultures
    T Thum
    Fraunhofer Institute of Toxicology and Aerosol Research, Center for Drug Research and Medical Biotechnology, 30625 Hannover, Germany
    Cardiovasc Toxicol 1:61-72. 2001
    ..05%) when BDM was added to the isolation and cultivation buffer. The results obtained with BDM warrant further investigation into its cytoprotective potential during ischemia and damage to the cytoskeleton...
  39. ncbi Effect of propionaldehyde or 2,3-butanedione additives on the mechanical properties of Bis-GMA analog-based composites
    Anuradha Prakki
    Center for Oral and Systemic Diseases, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7455, USA
    Dent Mater 25:26-32. 2009
    ....
  40. ncbi Blebbistatin: use as inhibitor of muscle contraction
    Gerrie P Farman
    Center for Cardiovascular Research, Department of Physiology and Biophysics M C 901, University of Illinois at Chicago, College of Medicine Chicago, Chicago, IL 60612 7342, USA
    Pflugers Arch 455:995-1005. 2008
    ....
  41. ncbi Industry-wide medical surveillance of California flavor manufacturing workers: Cross-sectional results
    Thomas J Kim
    Division of Environmental and Occupational Disease Control, California Department of Public Health CDPH, Richmond, California, USA
    Am J Ind Med 53:857-65. 2010
    ..Two cases of bronchiolitis obliterans in flavor manufacturing workers prompted California health and labor agencies to initiate industry-wide surveillance...
  42. pmc Respiratory toxicity of diacetyl in C57BL/6 mice
    Daniel L Morgan
    Respiratory Toxicology, Laboratory of Molecular Toxicology, National Toxicology Program National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Toxicol Sci 103:169-80. 2008
    b>Diacetyl, a component of artificial butter flavoring, is a potential etiological agent of obliterative bronchiolitis (OB); however, the toxic dose and mechanisms of toxicity remain controversial...
  43. ncbi Respiratory toxicologic pathology of inhaled diacetyl in sprague-dawley rats
    Ann F Hubbs
    Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, West Virginia 26505, USA
    Toxicol Pathol 36:330-44. 2008
    ..b>Diacetyl, an alpha-diketone, is a major component of these vapors...
  44. ncbi Airway obstruction related to diacetyl exposure at microwave popcorn production facilities
    J E Lockey
    Department of Environmental Health, University of Cincinnati, 3223 Eden Ave ML 0056, Cincinnati, OH 45267 0056, USA
    Eur Respir J 34:63-71. 2009
    ..Butter flavourings including diacetyl have been associated with these findings...
  45. ncbi 2,3-Butanedione monoxime (BDM) as a myosin inhibitor
    E Michael Ostap
    The Pennsylvania Muscle Institute, Department of Physiology, University of Pennsylvania School of Medicine, B400 Richards Building, Philadelphia, PA 19104 6085, USA
    J Muscle Res Cell Motil 23:305-8. 2002
    ..Therefore, BDM is not a general inhibitor of the myosin ATPase. BDM has a broad effect on many non-myosin proteins (many uncharacterized), and thus should not be used in whole-cell experiments as a myosin inhibitor...
  46. ncbi Effects of diacetyl monoxime and cytochalasin D on ventricular fibrillation in swine right ventricles
    M H Lee
    Division of Cardiology, Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, California 90048, USA
    Am J Physiol Heart Circ Physiol 280:H2689-96. 2001
    Whether or not the excitation-contraction (E-C) uncoupler diacetyl monoxime (DAM) and cytochalacin D (Cyto D) alter the ventricular fibrillation (VF) activation patterns is unclear...
  47. ncbi Clinical bronchiolitis obliterans in workers at a microwave-popcorn plant
    Kathleen Kreiss
    Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, WVa 26505, USA
    N Engl J Med 347:330-8. 2002
    ..No recognized cause was identified in the plant. Therefore, we medically evaluated current employees and assessed their occupational exposures...
  48. ncbi Presence of cytoskeleton proteins in parotid glands and their roles during secretion
    Tomoko Nashida
    Department of Biochemistry, School of Dentistry at Niigata, The Nippon Dental University, 1 8 Hamaura cho, Niigata 951 8580, Japan
    Arch Oral Biol 49:975-82. 2004
    ..We concluded from this study that the cytoskeleton played different roles in the beta-adrenergic and the muscarinic-cholinergic secretory processes...
  49. pmc Actin and non-muscle myosin II facilitate apical exocytosis of tear proteins in rabbit lacrimal acinar epithelial cells
    Galina V Jerdeva
    Department of Pharmaceutical Sciences, University of Southern California, 1985 Zonal Avenue, PSC 406A, Los Angeles, CA 90033, USA
    J Cell Sci 118:4797-812. 2005
    ....
  50. ncbi Myosin localization during meiosis I of crane-fly spermatocytes gives indications about its role in division
    Rosalind V Silverman-Gavrila
    Department of Biology, York University, Toronto, Ontario, Canada
    Cell Motil Cytoskeleton 55:97-113. 2003
    ....
  51. ncbi The association of peroxisomes with the developing cell plate in dividing onion root cells depends on actin microfilaments and myosin
    David A Collings
    Plant Cell Biology Group, Research School of Biological Sciences, Australian National University, GPO Box 475, ACT 2601 Canberra, Australia
    Planta 218:204-16. 2003
    ..Heynh., may reflect differences within the primary cell walls of these plants...
  52. ncbi Protection of ischemic myocardium in dogs using intracoronary 2,3-butanedione monoxime (BDM)
    Laurent Sebbag
    Pole de transplantation, Hopital Cardiologique Louis Pradel, BP Lyon Montchat, 69394 Lyon Cedex 3, France
    J Mol Cell Cardiol 35:165-76. 2003
    ..We tested whether 2,3-butanedione monoxime (BDM), a reversible inhibitor of actomyosin ATPase, given before coronary occlusion, limits infarct size in anesthetized open-chest dogs...
  53. pmc Myosin motors and not actin comets are mediators of the actin-based Golgi-to-endoplasmic reticulum protein transport
    Juan M Duran
    Departament de Biologia Cel lular i Anatomia Patològica, Facultat de Medicina, Institut d Investigacions Biomediques August Pi i Sunyer, Universitat de Barcelona, Spain
    Mol Biol Cell 14:445-59. 2003
    ....
  54. ncbi Two-photon molecular excitation imaging of Ca2+ transients in Langendorff-perfused mouse hearts
    Michael Rubart
    Wells Center for Pediatric Research, Indianapolis, Indiana 46202, USA
    Am J Physiol Cell Physiol 284:C1654-68. 2003
    ....
  55. ncbi Does 2,3-butanedione monoxime inhibit nonmuscle myosin?
    Arthur Forer
    Biology Department, York University, Toronto, Ontario, Canada
    Protoplasma 225:1-4. 2005
    ..We discuss how the two sets of apparently contradictory results might be resolved, and we suggest possible experiments that might clarify the contradictory interpretations...
  56. ncbi Skeletal muscle myosin cross-bridge cycling is necessary for myofibrillogenesis
    Indu Ramachandran
    Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri, 5100 Rockhill Road, Kansas City, MO 64110, USA
    Cell Motil Cytoskeleton 55:61-72. 2003
    ..Thus, our convergent results with BDM and BTS suggest that sarcomere assembly depends on active regulation of tension in the forming myofibril...
  57. ncbi Neurofilament transport is dependent on actin and myosin
    Cheolwha Jung
    Center for Cellular Neurobiology and Neurodegeneration Research, Departments of Biological Sciences and Biochemistry, University of Massachusetts Lowell, Lowell, Massachusetts 01854, USA
    J Neurosci 24:9486-96. 2004
    ..These findings suggest that some NF subunits may undergo axonal transport via myosin-mediated interactions with the actin cortex...
  58. pmc Interplay of troponin- and Myosin-based pathways of calcium activation in skeletal and cardiac muscle: the use of W7 as an inhibitor of thin filament activation
    Bishow B Adhikari
    Muscle Proteomics and Nanotechnology Section, Laboratory of Muscle Biology, National Institutes of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biophys J 86:359-70. 2004
    ..W7 holds much promise as a potent and reversible inhibitor of thin filament-mediated calcium activation of skeletal and cardiac muscle contraction...
  59. ncbi The contribution of the actomyosin motor to intracellular water transport in Elodea canadensis
    A V Anisimov
    Institute of Biochemistry and Biophysics, Kazan Scientific Center, Russian Academy of Sciences, Kazan, Tatarstan, Russia
    Dokl Biochem Biophys 392:292-3. 2003
  60. ncbi Involvement of myosin in intracellular motility and cytomorphogenesis in Micrasterias
    Anke Oertel
    Institute of Plant Physiology, University of Salzburg, Hellbrunnerstrasse 34, A 5020 Salzburg, Austria
    Cell Biol Int 27:977-86. 2003
    ..Together with the fact that other microtubules not connected to the F-actin system remained uninfluenced by BDM, this observation is evidence of an integrative function of myosin between the cytoskeleton elements...
  61. ncbi Role of adhesion and contraction in Rac 1-regulated endothelial barrier function in vivo and in vitro
    J Waschke
    Institute of Anatomy and Cell Biology, University of Wurzburg, Wurzburg, Germany
    Am J Physiol Heart Circ Physiol 287:H704-11. 2004
    ....
  62. ncbi Modulation of acto-myosin contractility in skeletal muscle myoblasts uncouples growth arrest from differentiation
    Jyotsna Dhawan
    Center for Cellular and Molecular Biology, Uppal Road, Hyderabad, 500 007, India
    J Cell Sci 117:3735-48. 2004
    ..We suggest that in myoblasts, the Rho pathway and regulation of acto-myosin contractility may define a control point for conditional uncoupling of differentiation and the cell cycle...
  63. ncbi Effects of BDM, [Ca2+]o, and temperature on the dynamic stiffness of quiescent cardiac trabeculae from rat
    R S Kirton
    Bioengineering Institute, University of Auckland, 70 Symonds St, Auckland, New Zealand
    Am J Physiol Heart Circ Physiol 288:H1662-7. 2005
    ..Experiments must be performed at sufficiently high temperatures (>25 degrees C) to ensure a low resting concentration of intracellular Ca(2+) or in the presence of an inhibitor of cross-bridge cycling...
  64. ncbi Modulation of actomyosin contractility by myosin light chain phosphorylation/dephosphorylation through Rho GTPases signaling specifies axon formation in neurons
    Yujin Kim
    Department of Life Science, Gwangju Institute of Science and Technology, 1 Oryong dong Buk gu, Gwangju 500 712, Republic of Korea
    Biochem Biophys Res Commun 318:579-87. 2004
    ..Our results suggest that MLC phosphorylation/dephosphorylation through Rho GTPases signaling modulates the actomyosin contractility, and then in turn provides a physiological tension in neurons to induce axon...
  65. ncbi The electrophysiological and mechanical effects of 2,3-butane-dione monoxime and cytochalasin-D in the Langendorff perfused rabbit heart
    S Kettlewell
    Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, Scotland, UK
    Exp Physiol 89:163-72. 2004
    ..This study has examined the electrophysiological consequences of a range of extracellular [Ca2+] (0.7-2.5 mmol l(-1)), 2,3-butane-dione monoxime (BDM; 1-20 mmol l(-1)) and cytochalasin-D (Cyto-D; 1-5 micromol l(-1))...
  66. ncbi Dynamic rearrangements of transvacuolar strands in BY-2 cells imply a role of myosin in remodeling the plant actin cytoskeleton
    Anja Hoffmann
    Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado, USA
    Protoplasma 224:201-10. 2004
    ..All effects of BDM on TVS dynamics were reversible upon removal of the drug. These results suggest a role for myosin motors in the rearrangement of TVS, which is likely to occur through their interaction with actin filaments...
  67. ncbi Caveat experimentor--is your myosin really inhibited?
    Margareta A Titus
    Nat Cell Biol 5:95. 2003
  68. ncbi Cytoskeletal scaffolds regulate riboflavin endocytosis and recycling in placental trophoblasts
    Vanessa M D'Souza
    Department of Pharmaceutical Sciences, University of Maryland, Baltimore, MD 21201, USA
    J Nutr Biochem 17:821-9. 2006
    ..In conclusion, apical RF trafficking in placental cells is tightly regulated by microtubules and supported by accessory actin involvement...
  69. pmc The role of myosin in vesicle transport during bovine chromaffin cell secretion
    Patricia Neco
    Instituto de Neurociencias, Centro Mixto CSIC Universidad Miguel Hernández, Campus de San Juan, 03550 Alicante, Spain
    Biochem J 368:405-13. 2002
    ....
  70. ncbi Specific changes to the mechanism of cell locomotion induced by overexpression of beta-actin
    M Peckham
    School of Biomedical Sciences, Worsley Building, University of Leeds, Leeds LS2 9JT, UK
    J Cell Sci 114:1367-77. 2001
    ....
  71. ncbi Dynamics of integrin clustering at focal contacts of endothelial cells studied by multimode imaging microscopy
    K Kawakami
    Department of Physiology, Nagoya University Graduate School of Medicine, 65 Tsurumai Showa-ku, Nagoya Aichi 4668550, Japan
    J Cell Sci 114:3125-35. 2001
    ..These results suggest that the clustering of integrins is mediated by actin-fiber-dependent translocation of integrins to the extending tip of focal contacts...
  72. ncbi Myosin inhibitors block accumulation movement of chloroplasts in Arabidopsis thaliana leaf cells
    H Paves
    Department of Gene Technology, Tallinn University of Technology, Tallinn, Estonia
    Protoplasma 230:165-9. 2007
    ..The results suggest that myosins have a role in A. thaliana chloroplast photorelocation...
  73. ncbi Actin dysfunction activates ERK1/2 and delays entry into mitosis in mammalian cells
    Kyunghee Lee
    Department of Biochemistry and Institute of Life Science and Biotechnology, College of Science, Yonsei University, Seoul, South Korea
    Cell Cycle 6:1487-95. 2007
    ....
  74. ncbi Actomyosin promotes cell plate alignment and late lateral expansion in Tradescantia stamen hair cells
    Tasha M Molchan
    The University of Massachusetts at Amherst, Biology Department, 221 Morrill Science Center III, Amherst, MA 01003, USA
    Planta 214:683-93. 2002
    ..Taken together, these results implicate myosin in the control of cell plate expansion and alignment...
  75. ncbi [Effect of actomyosin contractility on focal contacts of myofibroblasts and structure of stress fibers]
    V B Dugina
    A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Russia
    Tsitologiia 44:48-55. 2002
    ..Thus, actin-myosin contractility controls the structure of "supermature" focal contacts of myofibroblasts and alpha-SMA expression in stress fibers...
  76. ncbi Effects of anti-myosin drugs on anaphase chromosome movement and cytokinesis in crane-fly primary spermatocytes
    R V Silverman-Gavrila
    Biology Department, York University, Toronto, Ontario, Canada
    Cell Motil Cytoskeleton 50:180-97. 2001
    ..H-7 had no effect. These results confirm that the effects of BDM are on myosin and indicate that the myosin used for cytokinesis is activated starting from autosomal anaphase and continuing throughout cytokinesis...
  77. ncbi The myosin ATPase inhibitor 2,3-butanedione-2-monoxime disorganizes microtubules as well as F-actin in Saccharomyces cerevisiae
    K Chon
    Department of Biology, College of Science, Yonsei University, Seoul, Korea
    Cell Biol Toxicol 17:383-93. 2001
    ..cerevisiae...
  78. ncbi Actin and myosin inhibitors block elongation of kinetochore fibre stubs in metaphase crane-fly spermatocytes
    A Forer
    Biology Department, York University, Toronto, Ontario, Canada
    Protoplasma 232:79-85. 2007
    ..We suggest that actin and myosin act in concert with a spindle matrix to propel kinetochore fibres poleward, thereby causing stub elongation and generating anaphase chromosome movement in nonirradiated cells...
  79. ncbi Effects of deletion of muscle LIM protein on myocyte function
    Z Su
    Division of Cardiology, University of Utah Health Science Center, Salt Lake City, Utah 84132, USA
    Am J Physiol Heart Circ Physiol 280:H2665-73. 2001
    ..Thus a reduced [Ca2+]-induced shortening may be involved in the pathogenesis of myocardial dysfunction in this genetic model of heart failure...
  80. ncbi Calyculin-A, an inhibitor for protein phosphatases, induces cortical contraction in unfertilized sea urchin eggs
    Y Asano
    Graduate Program in Biophysics and Biochemistry, School of Science, University of Tokyo, Tokyo, Japan
    Cell Motil Cytoskeleton 48:245-61. 2001
    ..The phosphorylation of MRLC was also confirmed by a (32)P in vivo labeling experiment. The CL-A-induced cortical contraction may be a good model system for studying the mechanism of cytokinesis...
  81. ncbi Apparent elastic modulus and hysteresis of skeletal muscle cells throughout differentiation
    Amy M Collinsworth
    Department of Biomedical Engineering, 136 Hudson Hall, Duke University, Durham, NC 27708, USA
    Am J Physiol Cell Physiol 283:C1219-27. 2002
    ..Major contributors to changes in the transverse elastic modulus during differentiation were actin and myosin...
  82. ncbi Dopamine treatment of postischemic contractile dysfunction rapidly induces calcium-dependent pro-apoptotic signaling
    Christof Stamm
    Department of Cardiac Surgery, Children s Hospital, Harvard Medical School, Boston, Mass 02115, USA
    Circulation 106:I290-8. 2002
    ..We hypothesized that in a model of contractile dysfunction following ischemia, a commonly used catecholamine such as dopamine augments cardiomyocyte apoptosis via activation of calcium-dependent signaling cascades...
  83. ncbi Interaction of non-adherent suspended neutrophils to complement opsonized pathogens: a new assay using optical traps
    Tomoko Suzuki
    Department of Geriatric and Respiratory Medicine, Tohoku University School of Medicine, Sendai 980 8574, Japan
    Cell Res 16:887-94. 2006
    ....
  84. ncbi Mechanisms underlying ischemic diastolic dysfunction: relation between rigor, calcium homeostasis, and relaxation rate
    Niraj Varma
    Boston University School of Medicine, Boston 02118, USA
    Am J Physiol Heart Circ Physiol 284:H758-71. 2003
    ..Calcium resequestration capacity was preserved, and reduced relaxation velocity was not linked to upward arrow DCS...
  85. ncbi Myosin-based contraction is not necessary for cardiac c-looping in the chick embryo
    Mathieu C Rémond
    Department of Biomedical Engineering, Washington University, Campus Box 1097, St Louis, MO 63130, USA
    Anat Embryol (Berl) 211:443-54. 2006
    ..None of these drugs significantly affected looping during the studied stages. These results suggest that active contraction is not required for normal c-looping of the embryonic chick heart between stages 10 and 12...
  86. ncbi Living markers for actin block myosin-dependent motility of plant organelles and auxin
    Carola L Holweg
    Universitat Karlsruhe, Botanisches Institut I, Karlsruhe, Germany
    Cell Motil Cytoskeleton 64:69-81. 2007
    ....
  87. ncbi Formation of reactive oxygen species at increased contraction frequency in rat cardiomyocytes
    Frank R Heinzel
    Institut fur Pathophysiologie, Universitatsklinikum Essen, Hufelandstrasse 55, 45122 Essen, Germany
    Cardiovasc Res 71:374-82. 2006
    ..We studied ROS formation during increases in contraction frequency in isolated cardiomyocytes...
  88. ncbi Cell shape change precedes staurosporine-induced stabilization and accumulation of p27kip1
    Kayoko Nishi
    Department of Biological Chemistry Schol of Medicine, University of Davis, Davis, California 95616, USA
    Exp Cell Res 280:233-43. 2002
    ..The results indicate that the cell shape change caused by staurosporine correlates with the accumulation of p27kip1 and that staurosporine interferes with the p27kip1-specific proteolysis activity...
  89. ncbi Myosin light chain phosphorylation and growth cone motility
    John T Schmidt
    Department of Biological Sciences and Neuroscience Research Center, University at Albany SUNY, New York 12222, USA
    J Neurobiol 52:175-88. 2002
    ....
  90. ncbi Blebbistatin extends culture life of adult mouse cardiac myocytes and allows efficient and stable transgene expression
    Zhyldyz Kabaeva
    Dept of Molecular and Integrative Physiology, University of Michigan, 7623A Medical Science II, Ann Arbor, MI 48109 0622, USA
    Am J Physiol Heart Circ Physiol 294:H1667-74. 2008
    ..Furthermore, the unwanted effects of BDM on adult mouse cardiac myocytes, perhaps due to its nonspecific activities or action as a chemical phosphatase, can be avoided by using blebbistatin...
  91. ncbi Differential localization of unconventional myosin I and nonmuscle myosin II during B cell spreading
    Adriana Sumoza-Toledo
    Department of Molecular Biomedicine, Centro de Investigacion y Estudios Avanzados, Av IPN 2508 Col Zacatenco CP 07360, Mexico, D F, Mexico
    Exp Cell Res 312:3312-22. 2006
    ..This work shows that class I and class II myosin are expressed in B cells, are differentially distributed, and may participate in the morphological changes of these cells...
  92. ncbi Effects of heart isolation, voltage-sensitive dye, and electromechanical uncoupling agents on ventricular fibrillation
    Hao Qin
    Department of Physiology and Biophysics, University of Alabama, 1670 University Boulevard, Birmingham, AL 35294, USA
    Am J Physiol Heart Circ Physiol 284:H1818-26. 2003
    ..dye di-4-ANEPPS, and six more episodes were mapped after exposure to the electromechanical uncoupling agents diacetyl monoxime (DAM; 20 mmol/l, n = 6) or cytochalasin D (CytoD; 10 micromol/l, n = 6)...
  93. ncbi Developmental reorientation of transverse cortical microtubules to longitudinal directions: a role for actomyosin-based streaming and partial microtubule-membrane detachment
    Frank Sainsbury
    School of Biological Sciences, University of Sydney, Sydney, NSW 2006, Australia
    Plant J 56:116-31. 2008
    ....
  94. ncbi Both Ca2+ -dependent and -independent pathways are involved in rat hepatic stellate cell contraction and intrahepatic hyperresponsiveness to methoxamine
    Wim Laleman
    Dept of Hepatology, Univ Hospital Gasthuisberg, K U Leuven, Leuven, Belgium
    Am J Physiol Gastrointest Liver Physiol 292:G556-64. 2007
    ....
  95. pmc Spontaneous oscillation by hair bundles of the bullfrog's sacculus
    Pascal Martin
    Howard Hughes Medical Institute and Laboratory of Sensory Neuroscience, The Rockefeller University, New York, New York 10021 6399, USA
    J Neurosci 23:4533-48. 2003
    ..These observations accord with a model in which oscillations arise from the interplay of the hair bundle's negative stiffness with the activity of adaptation motors and with Ca 2+-dependent relaxation of gating springs...
  96. ncbi Contractile arrest reveals calcium-dependent stimulation of SERCA2a mRNA expression in cultured ventricular cardiomyocytes
    Ronald Vlasblom
    Laboratory for Physiology, Institute for Cardiovascular Research ICaR VU, VU University Medical Center, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands
    Cardiovasc Res 63:537-44. 2004
    ....
  97. ncbi Crucial role of cytoskeleton reorganization in the negative inotropic effect of chromogranin A-derived peptides in eel and frog hearts
    Rosa Mazza
    Department of Cell Biology, University of Calabria, 87030 Arcavacata di Rende Cs, Italy
    Regul Pept 138:145-51. 2007
    ..These results demonstrate that changes in cytoskeletal dynamics play a crucial role in the negative inotropic influence of VSs on eel and frog hearts...
  98. pmc Hair-bundle movements elicited by transepithelial electrical stimulation of hair cells in the sacculus of the bullfrog
    D Bozovic
    The Howard Hughes Medical Institute and Laboratory of Sensory Neuroscience, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 100:958-63. 2003
    ..A model for electrically driven bundle motion that incorporates the negative stiffness of the hair bundle as well as its two mechanisms of motility captures the essential features of the measured responses...
  99. ncbi Beer spoilage bacteria and hop resistance
    Kanta Sakamoto
    Fundamental Research Laboratory, Asahi Breweries, Ltd 1 21, Midori 1 chome, Moriya shi, Ibaraki 302 0106, Japan
    Int J Food Microbiol 89:105-24. 2003
    ..They can spoil beer by turbidity, acidity and the production of unfavorable smell such as diacetyl or hydrogen sulfide...
  100. pmc Purification and characterization of acetoin:2,6-dichlorophenolindophenol oxidoreductase, dihydrolipoamide dehydrogenase, and dihydrolipoamide acetyltransferase of the Pelobacter carbinolicus acetoin dehydrogenase enzyme system
    F B Oppermann
    Institut für Mikrobiologie der Georg August Universität, Gottingen, Federal Republic of Germany
    J Bacteriol 173:757-67. 1991
    ..it catalyzed a thiamine pyrophosphate-dependent oxidative-hydrolytic cleavage of acetoin, methylacetoin, and diacetyl. The combination of purified Ao:DCPIP OR, DHLTA, and DHLDH in the presence of thiamine pyrophosphate and the ..
  101. ncbi Characterization of a (2R,3R)-2,3-butanediol dehydrogenase as the Saccharomyces cerevisiae YAL060W gene product. Disruption and induction of the gene
    E Gonzalez
    Department of Biochemistry and Molecular Biology, Faculty of Sciences, Universitat Autonoma de Barcelona, E 08193 Bellaterra Barcelona, and Spain
    J Biol Chem 275:35876-85. 2000
    ..It was unable, however, to further oxidize these acetoins to diacetyl. In the presence of NADH, it could catalyze the stereospecific reduction of racemic acetoin ((3R/3S)- acetoin; K(..

Research Grants31

  1. HYPOXIC IMAGING GUIDED INTENSITY MODULATED RADIOTHERAPY
    KS CLIFFORD CHAO; Fiscal Year: 2003
    ..Cu(II)-diacetyl-bis(N4-methylthiosemicarbazone), or (Cu-ATSM), is a newly developed hypoxic marker for detecting tumor hypoxia ..
  2. Absorption, distribution, metabolism, excretion (ADME) of chemicals in mammals
    Rochelle W Tyl; Fiscal Year: 2009
    ..identification;toxicokinetics;Mechanisms of toxicity;2,3-butanedione;diacetyl;N,N-dimethylacetoacetamide;obliterative bronchiolitis;CYP;eugenol;safrole;estragole;anethole;myristicin;..
  3. Research on inhalation toxicology of environmental chemicals
    ROBERT O apos CONNER; Fiscal Year: 2010
    ..pentoxide;bleomycin, pulmonary function;ovalbumin, cigarette smoke, diacetyl, 2,3-pentanedione, benzene...
  4. Research on inhalation toxicology of environmental chemicals
    ROBERT O'CONNER; Fiscal Year: 2011
    ..pentoxide;bleomycin, pulmonary function;ovalbumin, cigarette smoke, diacetyl, 2,3-pentanedione, benzene...
  5. Regulation of p53 by TAF1
    Xuan Liu; Fiscal Year: 2012
    ..Upon DNA damage, K373/K382 diacetyl-p53 directly interacts with the double-bromodomain of TAF1 and recruits TAF1 to p21 promoter independent of other ..
  6. INTRA-TUMORAL METABOLIC HETEROGENEITY OF CERVICAL CANCER
    PERRY WAYNE GRIGSBY; Fiscal Year: 2013
    ..with 3'-Deoxy-3'-18F-fluorothymidine (FLT) (marker of cellular proliferation) and PET with copper(II)-diacetyl-bis(N4-methylthiosemicarbazone) (Cu-ATSM) (marker of assessment of hypoxia) to identify areas of metabolic ..
  7. Conceptual Prediction of Drug Bioactivities in Cell-Based Assays: cell-QSAR
    Stefan Balaz; Fiscal Year: 2013
    ..A novel surrogate solvent, diacetyl-PC (DAcPC, i.e...
  8. MINORITY PREDOCTORAL FELLOWSHIP PROGRAM
    NINITA BROWN; Fiscal Year: 2005
    ..Uncoupling agents such as diacetyl monoxime (DAM) are used to inhibit mechanical contraction...
  9. Positron Emission Tomography in Prostate Cancer
    Farrokh Dehdashti; Fiscal Year: 2008
    ..PET may also contribute significant prognostic information that may affect the decision to administer early adjuvant therapy to delay or prevent recurrence. [unreadable] [unreadable]..
  10. DISTRIBUTED OCCUPATIONAL KNOWLEDGE SYSTEM
    Philip Harber; Fiscal Year: 2001
    ..The process also yields valuable surveillance data including population segments often missed by other methods and describing functional impact of work rather than just occupationally caused disease. ..
  11. Occupational Medicine Activities and Skills: An Empiric Study
    Philip Harber; Fiscal Year: 2009
    ..Analyses will be both descriptive and address specific questions such as determining the impact of formal education and evaluating whether occupational preventive medicine is a homogeneous field or composed of distinct subgroups. ..
  12. REACTIVE INTERMEDIATES OF CARCINOGENESIS OF PAHS
    KENNETH LAALI; Fiscal Year: 2004
    ..DNA binding and mammalian cell mutagenicity studies on the substituted and derivatized-PAHs proposed for stable ion work will enhance the available literature data on structure/reactivity relationships. ..
  13. CATCH: Mechanochemistry and Regulation in Smooth Muscle
    Thomas Butler; Fiscal Year: 2008
    ..4) Determination of the extent of involvement of myosin crossbridges in catch force maintenance by measurements of changes in orientation of fluorescent probes attached to the regulatory light chain. ..
  14. NEUROTOXIC SUPERFUND CHEMICALS AND BIOMAKERS
    Peter Spencer; Fiscal Year: 2007
    ..abstract_text> ..
  15. Enteropathogenic E. coli Effects on Intestinal Function
    Gail A Hecht; Fiscal Year: 2010
    ..4. To evaluate the effect of EPEC infection on TJ structure and function in vivo using a mouse model and investigate the effector molecules and mechanisms involved. ..
  16. Fluorescence Lifetime and Steady-State Spectrometer
    E Ostap; Fiscal Year: 2003
    ..The core user group has extensive training in the operation, design, and maintenance of spectroscopic equipment. ..
  17. Neurobiol mechanism/acupuncture therapy/alcoholism
    Jiang Hong Ye; Fiscal Year: 2004
    ..This new knowledge will have a major impact on our understanding of how acupuncture works. The application of this knowledge is expected to improve the effectiveness of acupuncture treatment in alcohol addiction. ..
  18. CORTICAL OSTEOPONTIN EXPRESSION IN HYDRONEPHROSIS
    RUSSELL SCADUTO; Fiscal Year: 2002
    ..In these studies the renin angiotensin system will be manipulated with the use of an ACE inhibitor, enalapril, and an AT-1 blocker, losartan. ..
  19. Two-Photon Absorption Imaging by Laser Pulse Shaping
    Warren Warren; Fiscal Year: 2004
    ..abstract_text> ..
  20. Nasal dosimetry of diacetyl and butyric acid vapors
    John Morris; Fiscal Year: 2005
    ..Exposure to diacetyl (2,3 butadiene), a major component of butter flavoring vapors, results in necrosis of the nasal and trachea, but ..
  21. MECHANISM AND REGULATION OF ACTIN-BASED RETROGRADE FLOW
    JOHN HENSON; Fiscal Year: 2005
    ..4. To study the inducible transformation of coelomocytes from a lamellipodial to a filopodial form. ..
  22. Functional assessment of donor-host cell coupling
    MICHAEL RUBART VAN DER LOHE; Fiscal Year: 2009
    ..The studies proposed here will address fundamental hypotheses regarding the fate of donor cells following cellular transplantation into normal and injured hearts. ..
  23. Gustatory and olfactory receptors of Anopheles gambiae
    Hugh Robertson; Fiscal Year: 2006
    ..This work will also contribute to our overall understanding of the molecular basis of chemoreception in insects and other animals. [unreadable] [unreadable]..
  24. Innate Immune Responses in Obliterative Bronchiolitis
    Scott Palmer; Fiscal Year: 2006
    ..He will apply these skills to direct future investigations of patient oriented research problems in pulmonary medicine...
  25. ROS Defense Mechanisms in Brain Reoxygenation
    Sarah Milton; Fiscal Year: 2004
    ....
  26. ETHANOL EFFECTS ON GLYCINE RECEPTOR/CHANNEL FUNCTION
    Jiang Hong Ye; Fiscal Year: 2003
    ....
  27. Intermolecular Multiple-Quantum Coherence Effects in NMR
    Warren Warren; Fiscal Year: 2002
    ....
  28. MARINER TRANSPOSONS OF MAMMALS
    Hugh Robertson; Fiscal Year: 2001
    ..It will also reveal how occasionally particular copies of transposons can become integral parts of their host genetic complements. ..
  29. Study of Intrinsic Bladder Activity by Optical Imaging
    Anthony Kanai; Fiscal Year: 2007
    ..To study the neural-chemical control of spontaneous detrusor activity; and III.) To determine the origin of detrusor overactivity in partially obstructed bladders. ..
  30. Ethanol and mesolimbic GABAergic neurons
    Jiang Hong Ye; Fiscal Year: 2007
    ..Such results will provide valuable information on novel mechanisms underlying the addictive properties of alcohol and a firm scientific foundation for future neurobiological studies. [unreadable] [unreadable] [unreadable]..