Genomes and Genes
Summary: Carrier of aroma of butter, vinegar, coffee, and other foods.
Publications226 found, 100 shown here
- Aluminium causes variable responses in actin filament cytoskeleton of the root tip cells of Triticum turgidumG Frantzios
Department of Botany, Faculty of Biology, University of Athens, Athens, Greece
Protoplasma 225:129-40. 2005..These results favour the hypothesis that the actomyosin system in the Al-treated cells, among other roles, participates in the mechanism controlling callose deposition...
- Cytosolic localization of acetohydroxyacid synthase Ilv2 and its impact on diacetyl formation during beer fermentationSuvarna Dasari
Institut für Lebensmittelwissenschaft und Biotechnologie, Fg Gärungstechnologie 150f, Universitat Hohenheim, Garbenstr 23, D 70599 Stuttgart, Germany
Appl Environ Microbiol 77:727-31. 2011b>Diacetyl (2,3-butanedione) imparts an unpleasant "butterscotch-like" flavor to alcoholic beverages such as beer, and therefore its concentration needs to be reduced below the sensory threshold before packaging...
- Biocatalytic production of (2S,3S)-2,3-butanediol from diacetyl using whole cells of engineered Escherichia coliLixiang Li
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China
Bioresour Technol 115:111-6. 2012..Whole cells of the recombinant E. coli was used to produce (2S,3S)-2,3-BD from diacetyl. Under optimal conditions, high-optical-purity (2S,3S)-2,3-BD (purity >99%) was obtained with concentrations ..
- Production of S-acetoin from diacetyl by Escherichia coli transformant cells that express the diacetyl reductase gene of Paenibacillus polymyxa ZJ-9J Gao
Schol of Chemical and Biological Engineering, Yancheng Institute of Technology, Yancheng, China
Lett Appl Microbiol 57:274-81. 2013..In the current work, a gene encoding diacetyl reductase (DAR) from a Paenibacillus polymyxa strain ZJ-9 was cloned and expressed in Escherichia coli...
- Production of (3S)-acetoin from diacetyl by using stereoselective NADPH-dependent carbonyl reductase and glucose dehydrogenaseChao Gao
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China
Bioresour Technol 137:111-5. 2013..carbonyl reductase (Gox0644) from Gluconobacter oxydans DSM 2003 was confirmed to have a good ability to reduce diacetyl (DA) to produce (3S)-AC. In this work, the NADPH-dependent carbonyl reductase was expressed and purified...
- Determination of organic acids, sugars, diacetyl, and acetoin in cheese by high-performance liquid chromatographyG Zeppa
Dipartimento Valorizzazione e Protezione Risorse Agroforestali Settore Microbiologia e Industrie agrarie, Universita di Torino, Via L da Vinci 44, 10095, Grugliasco, Torino, Italy
J Agric Food Chem 49:2722-6. 2001..and hippuric), some free fatty acids (formic, acetic, propionic, butyric, isobutyric, valeric, and isovaleric), diacetyl, and acetoin were separated on an Aminex HPX-87H column using a simple isocratic HPLC method and identified by ..
- Genetic manipulation of the pathway for diacetyl metabolism in Lactococcus lactisS R Swindell
Institute of Food Research, Colney, United Kingdom
Appl Environ Microbiol 62:2641-3. 1996b>Diacetyl is an important food flavor compound produced by certain strains of citrate-metabolizing lactic acid bacteria. Citrate is converted to pyruvate, from which diacetyl is produced via intermediate alpha-acetolactate...
- Identification of Sc-type ILV6 as a target to reduce diacetyl formation in lager brewers' yeastC T Duong
Department of Microbiology and Genetics, Berlin University of Technology, Seestr 13, D 13353 Berlin, Germany
Metab Eng 13:638-47. 2011b>Diacetyl causes an unwanted buttery off-flavor in lager beer. It is spontaneously generated from α-acetolactate, an intermediate of yeast's valine biosynthesis released during the main beer fermentation...
- Separation of propulsive and adhesive traction stresses in locomoting keratocytesT Oliver
Department of Mechanical Engineering and Materials Science, Duke University, Durham, North Carolina 27708 0300, USA
J Cell Biol 145:589-604. 1999....
- Activation of the diacetyl/acetoin pathway in Lactococcus lactis subsp. lactis bv. diacetylactis CRL264 by acidic growthNieves García-Quintáns
Departamento de Ciencia de Proteínas, Centro de Investigaciones Biologicas, Ramiro de Maeztu 9, 28040 Madrid, Spain
Appl Environ Microbiol 74:1988-96. 2008..a fermentative microorganism, which cometabolizes glucose and citrate to yield aroma compounds through the diacetyl/acetoin biosynthetic pathway. Our previous results have shown that under acidic growth Lactococcus bv...
- Lactococcus lactis as a cell factory for high-level diacetyl productionJ Hugenholtz
Wageningen Centre for Food Sciences and NIZO Food Research, 6710 BA Ede, The Netherlands
Appl Environ Microbiol 66:4112-4. 2000We report the engineering of Lactococcus lactis for the efficient conversion of sugar into diacetyl by combining NADH-oxidase overproduction and alpha-acetolactate decarboxylase inactivation...
- The 'buttery' attribute of wine--diacetyl--desirability, spoilage and beyondEveline J Bartowsky
The Australian Wine Research Institute, PO Box 197, Glen Osmond, SA 5064, Australia
Int J Food Microbiol 96:235-52. 2004The diketone, diacetyl, is a major flavour metabolite produced by lactic acid bacteria (LAB)...
- Diacetyl and alpha-acetolactate overproduction by Lactococcus lactis subsp. lactis biovar diacetylactis mutants that are deficient in alpha-acetolactate decarboxylase and have a low lactate dehydrogenase activityC Monnet
Laboratoire de Génie et Microbiologie des Procédés Alimentaires, Institut National de la Recherche Agronomique, 78850 Thiverval Grignon, France
Appl Environ Microbiol 66:5518-20. 2000..biovar diacetylactis strains are utilized in several industrial processes for producing the flavoring compound diacetyl or its precursor alpha-acetolactate...
- Ca2+-independent alterations in diastolic sarcomere length and relaxation kinetics in a mouse model of lipotoxic diabetic cardiomyopathyThomas P Flagg
Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
Circ Res 104:95-103. 2009....
- Production of L-2,3-butanediol by a new pathway constructed in Escherichia coliS Ui
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Kofu, Yamanashi, Japan
Lett Appl Microbiol 39:533-7. 2004..To rectify this situation, we attempted to produce L-BD from diacetyl (DA) by producing simultaneous expression of diacetyl reductase (DAR) and L-2,3-butanediol dehydrogenase (BDH) ..
- Diacetyl and acetoin production from whey permeate using engineered Lactobacillus caseiInmaculada Nadal
Laboratorio de Bacterias Lacticas y Probioticos, Instituto de Agroquimica y Tecnologia de Alimentos CSIC, 46100 Burjassot, Valencia, Spain
J Ind Microbiol Biotechnol 36:1233-7. 2009The capability of Lactobacillus casei to produce the flavor-related compounds diacetyl and acetoin from whey permeate has been examined by a metabolic engineering approach. An L...
- Effects of mechanical uncouplers, diacetyl monoxime, and cytochalasin-D on the electrophysiology of perfused mouse heartsLinda C Baker
Dept of Cell Biology and Physiology, S314 Biomedical Science Tower, University of Pittsburgh School of Medicine, 3500 Terrace Street, Pittsburgh, PA 15261, USA
Am J Physiol Heart Circ Physiol 287:H1771-9. 2004Chemical uncouplers diacetyl monoxime (DAM) and cytochalasin D (cyto-D) are used to abolish cardiac contractions in optical studies, yet alter intracellular Ca(2+) concentration ([Ca(2+)](i)) handling and vulnerability to arrhythmias in a ..
- Induction of chemotaxis to sodium chloride and diacetyl and thermotaxis defects by microcystin-LR exposure in nematode Caenorhabditis elegansYunhui Li
Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Nanjing 210009, China
J Environ Sci (China) 21:971-9. 2009..In the present study, we provided evidence to indicate the neurotoxicity on chemotaxis to NaCl and diacetyl, and thermotaxis from MC-LR exposure to C. elegans...
- odr-10 encodes a seven transmembrane domain olfactory receptor required for responses to the odorant diacetylP Sengupta
Howard Hughes Medical Institute, Programs in Developmental Biology, Neuroscience, and Genetics, University of California, San Francisco, 94143 0452, USA
Cell 84:899-909. 1996..We show that the C. elegans odr-10 gene is likely to encode a receptor for the odorant diacetyl. odr-10 mutants have a specific defect in chemotaxis to diacetyl, one of several odorants detected by the AWA ..
- Fine tuning of the lactate and diacetyl production through promoter engineering in Lactococcus lactisTingting Guo
State Key Laboratory of Microbial Technology, Shandong University, Jinan, People s Republic of China
PLoS ONE 7:e36296. 2012..For fine tuning of the distribution of glycolytic flux at the pyruvate branch from lactate to diacetyl and balancing the production of the two metabolites under aerobic conditions, a constitutive promoter library was ..
- Engineering of cofactor regeneration enhances (2S,3S)-2,3-butanediol production from diacetylYu Wang
1 State Key Laboratory of Microbial Metabolism, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People s Republic of China 2 State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People s Republic of China
Sci Rep 3:2643. 2013..Introduction of FDH resulted in higher (2S,3S)-2,3-BD concentration, productivity and yield from diacetyl, and large increase in the intracellular NADH concentration...
- New insights into Lactococcus lactis diacetyl- and acetoin-producing strains isolated from diverse originsDelphine Passerini
Universite de Toulouse, Universite Paul Sabatier, 118 route de Narbonne, Toulouse, France
Int J Food Microbiol 160:329-36. 2013..lactis biovar diacetylactis strains are used in the dairy industry for generating acetoin and notably diacetyl which imparts a high level of buttery flavor notes...
- A proposal for a safe exposure level for diacetylDavid S Egilman
Never Again Consulting, Attleboro, MA, USA
Int J Occup Environ Health 17:122-34. 2011b>Diacetyl is a naturally occurring compound that has been used in concentrated form as a food additive, particularly in butter flavorings...
- Reaction of the butter flavorant diacetyl (2,3-butanedione) with N-α-acetylarginine: a model for epitope formation with pulmonary proteins in the etiology of obliterative bronchiolitisJames M Mathews
RTI International, P O Box 12194, Research Triangle Park, North Carolina 27709, United States
J Agric Food Chem 58:12761-8. 2010The butter flavorant diacetyl (2,3-butanedione) is implicated in causing obliterative bronchiolitis in microwave popcorn plant workers...
- Blood and urinary levels of ethanol, acetaldehyde, and C4 compounds such as diacetyl, acetoin, and 2,3-butanediol in normal male students after ethanol ingestionM Otsuka
Faculty of Pharmaceutical Sciences, Okayama University, Japan
Alcohol 17:119-24. 1999..The student with the inactive form of ALDH2 was flushed and his levels of 2,3-butanediol and acetaldehyde in blood and urine were found to be the highest...
- Construction of self-cloning bottom-fermenting yeast with low vicinal diketone production by the homo-integration of ILV5K Kusunoki
Research Laboratories for Brewing, Asahi Breweries Ltd, Ibaraki, Japan
Yeast 29:435-42. 2012The vicinal diketones (VDK), such as diacetyl and 2,3-pentandione, impart an unpleasant butter-like flavour to beer...
- Correcting diacetyl concentrations from air samples collected with NIOSH method 2557Jean Cox-Ganser
Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, 1095 Willowdale Road, Morgantown, WV 26505 2888, USA
J Occup Environ Hyg 8:59-70. 2011b>Diacetyl (2,3-butanedione), a diketone chemical used to impart a buttery taste in many flavoring mixtures, has been associated with bronchiolitis obliterans in several industrial settings...
- Method for the simultaneous assay of diacetyl and acetoin in the presence of alpha-acetolactate: application in determining the kinetic parameters for the decomposition of alpha-acetolactateKen Kobayashi
National Research Institute of Brewing, 3 7 1 Kagamiyama, Higashi Hiroshima, Hiroshima 739 0046, Japan
J Biosci Bioeng 99:502-7. 2005A simultaneous assay method for diacetyl and acetoin was developed to investigate the formation of diacetyl during the brewing of alcoholic beverages...
- Influence of additives on the properties of Bis-GMA/Bis-GMA analog comonomers and corresponding copolymersAnuradha Prakki
Department of Operative Dentistry, Endodontics and Dental Materials, University of Sao Paulo, Bauru, Sao Paulo, Brazil
Dent Mater 23:1199-204. 2007....
- Inhalation dosimetry of diacetyl and butyric acid, two components of butter flavoring vaporsJohn B Morris
Toxicology Program, Department of Pharmaceutical Sciences, University of Connecticut, Storrs, Connecticut 06269, USA
Toxicol Sci 108:173-83. 2009..The goal of the current study was to characterize inhalation dosimetric patterns of diacetyl and butyric acid, two components of BFV, and to develop a hybrid computational fluid dynamic-physiologically ..
- Role of diacetyl metabolite in alcohol toxicity and addiction via electron transfer and oxidative stressPeter Kovacic
Department of Chemistry, San Diego State University, San Diego, CA, USA
Arch Toxicol 79:123-8. 2005..Metabolism affords mainly acetic acid via acetaldehyde. A minor metabolite, diacetyl (an alpha-dicarbonyl), arises from the aldehyde...
- Developmental changes in chemotactic response and choice of two attractants, sodium acetate and diacetyl, in the nematode Caenorhabditis elegansTetsuya Matsuura
Department of Welfare Engineering, Faculty of Engineering, Iwate University, 4 3 5 Ueda Morioka 020 8551, Japan
Comp Biochem Physiol A Mol Integr Physiol 147:920-7. 2007..behavior of the nematode Caenorhabditis elegans to chemical attractants, water-soluble sodium acetate and odorant diacetyl, was investigated using nematodes at various developmental stages to examine the effects of postembryonic ..
- Formation of diacetyl and acetoin by Lactococcus lactis via aspartate catabolismD Le Bars
Unité de Biochimie Bactérienne, UR477, INRA, Jouy en Josas, France
J Appl Microbiol 104:171-7. 2008To verify whether diacetyl can be produced by Lactococcus lactis via amino acid catabolism, and to investigate the impact of the pH on the conversion.
- Use of diacetyl to reduce the load of Vibrio vulnificus in the Eastern oyster, Crassostrea virginicaJ M Birkenhauer
Department of Biology, University of North Carolina at Charlotte, 9201 University City Boulevard, Charlotte, North Carolina 28223, USA
J Food Prot 66:38-43. 2003..conducted in our laboratory have examined various generally recognized as safe compounds and have determined that diacetyl, a component of butter, is among the most effective of these compounds in reducing loads of V...
- In vitro wear, surface roughness and hardness of propanal-containing and diacetyl-containing novel composites and copolymers based on bis-GMA analogsAnuradha Prakki
Department of Operative Dentistry, Endodontics and Dental Materials, University of Sao Paulo, Bauru Dental School, Bauru, São Paulo 17043 170, Brazil
Dent Mater 24:410-7. 2008....
- Bronchiolitis obliterans syndrome in chemical workers producing diacetyl for food flavoringsFrits G B G J van Rooy
Netherlands Expertise Centre for Occupational Respiratory Disorders, Utrecht, The Netherlands
Am J Respir Crit Care Med 176:498-504. 2007Workers in microwave popcorn plants are at risk of developing bronchiolitis obliterans associated with exposure to butter flavoring volatiles, including diacetyl.
- Localised depletion of polymerised actin at the front of Walker carcinosarcoma cells increases the speed of locomotionHansuli Keller
Institute of Pathology, University of Bern, Bern, Switzerland
Cell Motil Cytoskeleton 53:189-202. 2002..They are best compatible with a cortical contraction/frontal expansion model, but they are not compatible with models implying that actin polymerisation or actomyosin contraction at the front drive locomotion of the cells studied...
- Butanedione monoxime increases the viability and yield of adult cardiomyocytes in primary culturesT Thum
Fraunhofer Institute of Toxicology and Aerosol Research, Center for Drug Research and Medical Biotechnology, 30625 Hannover, Germany
Cardiovasc Toxicol 1:61-72. 2001..05%) when BDM was added to the isolation and cultivation buffer. The results obtained with BDM warrant further investigation into its cytoprotective potential during ischemia and damage to the cytoskeleton...
- Effect of propionaldehyde or 2,3-butanedione additives on the mechanical properties of Bis-GMA analog-based compositesAnuradha Prakki
Center for Oral and Systemic Diseases, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7455, USA
Dent Mater 25:26-32. 2009....
- Blebbistatin: use as inhibitor of muscle contractionGerrie P Farman
Center for Cardiovascular Research, Department of Physiology and Biophysics M C 901, University of Illinois at Chicago, College of Medicine Chicago, Chicago, IL 60612 7342, USA
Pflugers Arch 455:995-1005. 2008....
- Industry-wide medical surveillance of California flavor manufacturing workers: Cross-sectional resultsThomas J Kim
Division of Environmental and Occupational Disease Control, California Department of Public Health CDPH, Richmond, California, USA
Am J Ind Med 53:857-65. 2010..Two cases of bronchiolitis obliterans in flavor manufacturing workers prompted California health and labor agencies to initiate industry-wide surveillance...
- Respiratory toxicity of diacetyl in C57BL/6 miceDaniel L Morgan
Respiratory Toxicology, Laboratory of Molecular Toxicology, National Toxicology Program National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
Toxicol Sci 103:169-80. 2008b>Diacetyl, a component of artificial butter flavoring, is a potential etiological agent of obliterative bronchiolitis (OB); however, the toxic dose and mechanisms of toxicity remain controversial...
- Respiratory toxicologic pathology of inhaled diacetyl in sprague-dawley ratsAnn F Hubbs
Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, West Virginia 26505, USA
Toxicol Pathol 36:330-44. 2008..b>Diacetyl, an alpha-diketone, is a major component of these vapors...
- Airway obstruction related to diacetyl exposure at microwave popcorn production facilitiesJ E Lockey
Department of Environmental Health, University of Cincinnati, 3223 Eden Ave ML 0056, Cincinnati, OH 45267 0056, USA
Eur Respir J 34:63-71. 2009..Butter flavourings including diacetyl have been associated with these findings...
- 2,3-Butanedione monoxime (BDM) as a myosin inhibitorE Michael Ostap
The Pennsylvania Muscle Institute, Department of Physiology, University of Pennsylvania School of Medicine, B400 Richards Building, Philadelphia, PA 19104 6085, USA
J Muscle Res Cell Motil 23:305-8. 2002..Therefore, BDM is not a general inhibitor of the myosin ATPase. BDM has a broad effect on many non-myosin proteins (many uncharacterized), and thus should not be used in whole-cell experiments as a myosin inhibitor...
- Effects of diacetyl monoxime and cytochalasin D on ventricular fibrillation in swine right ventriclesM H Lee
Division of Cardiology, Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, California 90048, USA
Am J Physiol Heart Circ Physiol 280:H2689-96. 2001Whether or not the excitation-contraction (E-C) uncoupler diacetyl monoxime (DAM) and cytochalacin D (Cyto D) alter the ventricular fibrillation (VF) activation patterns is unclear...
- Clinical bronchiolitis obliterans in workers at a microwave-popcorn plantKathleen Kreiss
Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, WVa 26505, USA
N Engl J Med 347:330-8. 2002..No recognized cause was identified in the plant. Therefore, we medically evaluated current employees and assessed their occupational exposures...
- Presence of cytoskeleton proteins in parotid glands and their roles during secretionTomoko Nashida
Department of Biochemistry, School of Dentistry at Niigata, The Nippon Dental University, 1 8 Hamaura cho, Niigata 951 8580, Japan
Arch Oral Biol 49:975-82. 2004..We concluded from this study that the cytoskeleton played different roles in the beta-adrenergic and the muscarinic-cholinergic secretory processes...
- Actin and non-muscle myosin II facilitate apical exocytosis of tear proteins in rabbit lacrimal acinar epithelial cellsGalina V Jerdeva
Department of Pharmaceutical Sciences, University of Southern California, 1985 Zonal Avenue, PSC 406A, Los Angeles, CA 90033, USA
J Cell Sci 118:4797-812. 2005....
- Myosin localization during meiosis I of crane-fly spermatocytes gives indications about its role in divisionRosalind V Silverman-Gavrila
Department of Biology, York University, Toronto, Ontario, Canada
Cell Motil Cytoskeleton 55:97-113. 2003....
- The association of peroxisomes with the developing cell plate in dividing onion root cells depends on actin microfilaments and myosinDavid A Collings
Plant Cell Biology Group, Research School of Biological Sciences, Australian National University, GPO Box 475, ACT 2601 Canberra, Australia
Planta 218:204-16. 2003..Heynh., may reflect differences within the primary cell walls of these plants...
- Protection of ischemic myocardium in dogs using intracoronary 2,3-butanedione monoxime (BDM)Laurent Sebbag
Pole de transplantation, Hopital Cardiologique Louis Pradel, BP Lyon Montchat, 69394 Lyon Cedex 3, France
J Mol Cell Cardiol 35:165-76. 2003..We tested whether 2,3-butanedione monoxime (BDM), a reversible inhibitor of actomyosin ATPase, given before coronary occlusion, limits infarct size in anesthetized open-chest dogs...
- Myosin motors and not actin comets are mediators of the actin-based Golgi-to-endoplasmic reticulum protein transportJuan M Duran
Departament de Biologia Cel lular i Anatomia Patològica, Facultat de Medicina, Institut d Investigacions Biomediques August Pi i Sunyer, Universitat de Barcelona, Spain
Mol Biol Cell 14:445-59. 2003....
- Two-photon molecular excitation imaging of Ca2+ transients in Langendorff-perfused mouse heartsMichael Rubart
Wells Center for Pediatric Research, Indianapolis, Indiana 46202, USA
Am J Physiol Cell Physiol 284:C1654-68. 2003....
- Does 2,3-butanedione monoxime inhibit nonmuscle myosin?Arthur Forer
Biology Department, York University, Toronto, Ontario, Canada
Protoplasma 225:1-4. 2005..We discuss how the two sets of apparently contradictory results might be resolved, and we suggest possible experiments that might clarify the contradictory interpretations...
- Skeletal muscle myosin cross-bridge cycling is necessary for myofibrillogenesisIndu Ramachandran
Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri, 5100 Rockhill Road, Kansas City, MO 64110, USA
Cell Motil Cytoskeleton 55:61-72. 2003..Thus, our convergent results with BDM and BTS suggest that sarcomere assembly depends on active regulation of tension in the forming myofibril...
- Neurofilament transport is dependent on actin and myosinCheolwha Jung
Center for Cellular Neurobiology and Neurodegeneration Research, Departments of Biological Sciences and Biochemistry, University of Massachusetts Lowell, Lowell, Massachusetts 01854, USA
J Neurosci 24:9486-96. 2004..These findings suggest that some NF subunits may undergo axonal transport via myosin-mediated interactions with the actin cortex...
- Interplay of troponin- and Myosin-based pathways of calcium activation in skeletal and cardiac muscle: the use of W7 as an inhibitor of thin filament activationBishow B Adhikari
Muscle Proteomics and Nanotechnology Section, Laboratory of Muscle Biology, National Institutes of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Biophys J 86:359-70. 2004..W7 holds much promise as a potent and reversible inhibitor of thin filament-mediated calcium activation of skeletal and cardiac muscle contraction...
- The contribution of the actomyosin motor to intracellular water transport in Elodea canadensisA V Anisimov
Institute of Biochemistry and Biophysics, Kazan Scientific Center, Russian Academy of Sciences, Kazan, Tatarstan, Russia
Dokl Biochem Biophys 392:292-3. 2003
- Involvement of myosin in intracellular motility and cytomorphogenesis in MicrasteriasAnke Oertel
Institute of Plant Physiology, University of Salzburg, Hellbrunnerstrasse 34, A 5020 Salzburg, Austria
Cell Biol Int 27:977-86. 2003..Together with the fact that other microtubules not connected to the F-actin system remained uninfluenced by BDM, this observation is evidence of an integrative function of myosin between the cytoskeleton elements...
- Role of adhesion and contraction in Rac 1-regulated endothelial barrier function in vivo and in vitroJ Waschke
Institute of Anatomy and Cell Biology, University of Wurzburg, Wurzburg, Germany
Am J Physiol Heart Circ Physiol 287:H704-11. 2004....
- Modulation of acto-myosin contractility in skeletal muscle myoblasts uncouples growth arrest from differentiationJyotsna Dhawan
Center for Cellular and Molecular Biology, Uppal Road, Hyderabad, 500 007, India
J Cell Sci 117:3735-48. 2004..We suggest that in myoblasts, the Rho pathway and regulation of acto-myosin contractility may define a control point for conditional uncoupling of differentiation and the cell cycle...
- Effects of BDM, [Ca2+]o, and temperature on the dynamic stiffness of quiescent cardiac trabeculae from ratR S Kirton
Bioengineering Institute, University of Auckland, 70 Symonds St, Auckland, New Zealand
Am J Physiol Heart Circ Physiol 288:H1662-7. 2005..Experiments must be performed at sufficiently high temperatures (>25 degrees C) to ensure a low resting concentration of intracellular Ca(2+) or in the presence of an inhibitor of cross-bridge cycling...
- Modulation of actomyosin contractility by myosin light chain phosphorylation/dephosphorylation through Rho GTPases signaling specifies axon formation in neuronsYujin Kim
Department of Life Science, Gwangju Institute of Science and Technology, 1 Oryong dong Buk gu, Gwangju 500 712, Republic of Korea
Biochem Biophys Res Commun 318:579-87. 2004..Our results suggest that MLC phosphorylation/dephosphorylation through Rho GTPases signaling modulates the actomyosin contractility, and then in turn provides a physiological tension in neurons to induce axon...
- The electrophysiological and mechanical effects of 2,3-butane-dione monoxime and cytochalasin-D in the Langendorff perfused rabbit heartS Kettlewell
Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, Scotland, UK
Exp Physiol 89:163-72. 2004..This study has examined the electrophysiological consequences of a range of extracellular [Ca2+] (0.7-2.5 mmol l(-1)), 2,3-butane-dione monoxime (BDM; 1-20 mmol l(-1)) and cytochalasin-D (Cyto-D; 1-5 micromol l(-1))...
- Dynamic rearrangements of transvacuolar strands in BY-2 cells imply a role of myosin in remodeling the plant actin cytoskeletonAnja Hoffmann
Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado, USA
Protoplasma 224:201-10. 2004..All effects of BDM on TVS dynamics were reversible upon removal of the drug. These results suggest a role for myosin motors in the rearrangement of TVS, which is likely to occur through their interaction with actin filaments...
- Caveat experimentor--is your myosin really inhibited?Margareta A Titus
Nat Cell Biol 5:95. 2003
- Cytoskeletal scaffolds regulate riboflavin endocytosis and recycling in placental trophoblastsVanessa M D'Souza
Department of Pharmaceutical Sciences, University of Maryland, Baltimore, MD 21201, USA
J Nutr Biochem 17:821-9. 2006..In conclusion, apical RF trafficking in placental cells is tightly regulated by microtubules and supported by accessory actin involvement...
- The role of myosin in vesicle transport during bovine chromaffin cell secretionPatricia Neco
Instituto de Neurociencias, Centro Mixto CSIC Universidad Miguel Hernández, Campus de San Juan, 03550 Alicante, Spain
Biochem J 368:405-13. 2002....
- Specific changes to the mechanism of cell locomotion induced by overexpression of beta-actinM Peckham
School of Biomedical Sciences, Worsley Building, University of Leeds, Leeds LS2 9JT, UK
J Cell Sci 114:1367-77. 2001....
- Dynamics of integrin clustering at focal contacts of endothelial cells studied by multimode imaging microscopyK Kawakami
Department of Physiology, Nagoya University Graduate School of Medicine, 65 Tsurumai Showa-ku, Nagoya Aichi 4668550, Japan
J Cell Sci 114:3125-35. 2001..These results suggest that the clustering of integrins is mediated by actin-fiber-dependent translocation of integrins to the extending tip of focal contacts...
- Myosin inhibitors block accumulation movement of chloroplasts in Arabidopsis thaliana leaf cellsH Paves
Department of Gene Technology, Tallinn University of Technology, Tallinn, Estonia
Protoplasma 230:165-9. 2007..The results suggest that myosins have a role in A. thaliana chloroplast photorelocation...
- Actin dysfunction activates ERK1/2 and delays entry into mitosis in mammalian cellsKyunghee Lee
Department of Biochemistry and Institute of Life Science and Biotechnology, College of Science, Yonsei University, Seoul, South Korea
Cell Cycle 6:1487-95. 2007....
- Actomyosin promotes cell plate alignment and late lateral expansion in Tradescantia stamen hair cellsTasha M Molchan
The University of Massachusetts at Amherst, Biology Department, 221 Morrill Science Center III, Amherst, MA 01003, USA
Planta 214:683-93. 2002..Taken together, these results implicate myosin in the control of cell plate expansion and alignment...
- [Effect of actomyosin contractility on focal contacts of myofibroblasts and structure of stress fibers]V B Dugina
A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Russia
Tsitologiia 44:48-55. 2002..Thus, actin-myosin contractility controls the structure of "supermature" focal contacts of myofibroblasts and alpha-SMA expression in stress fibers...
- Effects of anti-myosin drugs on anaphase chromosome movement and cytokinesis in crane-fly primary spermatocytesR V Silverman-Gavrila
Biology Department, York University, Toronto, Ontario, Canada
Cell Motil Cytoskeleton 50:180-97. 2001..H-7 had no effect. These results confirm that the effects of BDM are on myosin and indicate that the myosin used for cytokinesis is activated starting from autosomal anaphase and continuing throughout cytokinesis...
- The myosin ATPase inhibitor 2,3-butanedione-2-monoxime disorganizes microtubules as well as F-actin in Saccharomyces cerevisiaeK Chon
Department of Biology, College of Science, Yonsei University, Seoul, Korea
Cell Biol Toxicol 17:383-93. 2001..cerevisiae...
- Actin and myosin inhibitors block elongation of kinetochore fibre stubs in metaphase crane-fly spermatocytesA Forer
Biology Department, York University, Toronto, Ontario, Canada
Protoplasma 232:79-85. 2007..We suggest that actin and myosin act in concert with a spindle matrix to propel kinetochore fibres poleward, thereby causing stub elongation and generating anaphase chromosome movement in nonirradiated cells...
- Effects of deletion of muscle LIM protein on myocyte functionZ Su
Division of Cardiology, University of Utah Health Science Center, Salt Lake City, Utah 84132, USA
Am J Physiol Heart Circ Physiol 280:H2665-73. 2001..Thus a reduced [Ca2+]-induced shortening may be involved in the pathogenesis of myocardial dysfunction in this genetic model of heart failure...
- Calyculin-A, an inhibitor for protein phosphatases, induces cortical contraction in unfertilized sea urchin eggsY Asano
Graduate Program in Biophysics and Biochemistry, School of Science, University of Tokyo, Tokyo, Japan
Cell Motil Cytoskeleton 48:245-61. 2001..The phosphorylation of MRLC was also confirmed by a (32)P in vivo labeling experiment. The CL-A-induced cortical contraction may be a good model system for studying the mechanism of cytokinesis...
- Apparent elastic modulus and hysteresis of skeletal muscle cells throughout differentiationAmy M Collinsworth
Department of Biomedical Engineering, 136 Hudson Hall, Duke University, Durham, NC 27708, USA
Am J Physiol Cell Physiol 283:C1219-27. 2002..Major contributors to changes in the transverse elastic modulus during differentiation were actin and myosin...
- Dopamine treatment of postischemic contractile dysfunction rapidly induces calcium-dependent pro-apoptotic signalingChristof Stamm
Department of Cardiac Surgery, Children s Hospital, Harvard Medical School, Boston, Mass 02115, USA
Circulation 106:I290-8. 2002..We hypothesized that in a model of contractile dysfunction following ischemia, a commonly used catecholamine such as dopamine augments cardiomyocyte apoptosis via activation of calcium-dependent signaling cascades...
- Interaction of non-adherent suspended neutrophils to complement opsonized pathogens: a new assay using optical trapsTomoko Suzuki
Department of Geriatric and Respiratory Medicine, Tohoku University School of Medicine, Sendai 980 8574, Japan
Cell Res 16:887-94. 2006....
- Mechanisms underlying ischemic diastolic dysfunction: relation between rigor, calcium homeostasis, and relaxation rateNiraj Varma
Boston University School of Medicine, Boston 02118, USA
Am J Physiol Heart Circ Physiol 284:H758-71. 2003..Calcium resequestration capacity was preserved, and reduced relaxation velocity was not linked to upward arrow DCS...
- Myosin-based contraction is not necessary for cardiac c-looping in the chick embryoMathieu C Rémond
Department of Biomedical Engineering, Washington University, Campus Box 1097, St Louis, MO 63130, USA
Anat Embryol (Berl) 211:443-54. 2006..None of these drugs significantly affected looping during the studied stages. These results suggest that active contraction is not required for normal c-looping of the embryonic chick heart between stages 10 and 12...
- Living markers for actin block myosin-dependent motility of plant organelles and auxinCarola L Holweg
Universitat Karlsruhe, Botanisches Institut I, Karlsruhe, Germany
Cell Motil Cytoskeleton 64:69-81. 2007....
- Formation of reactive oxygen species at increased contraction frequency in rat cardiomyocytesFrank R Heinzel
Institut fur Pathophysiologie, Universitatsklinikum Essen, Hufelandstrasse 55, 45122 Essen, Germany
Cardiovasc Res 71:374-82. 2006..We studied ROS formation during increases in contraction frequency in isolated cardiomyocytes...
- Cell shape change precedes staurosporine-induced stabilization and accumulation of p27kip1Kayoko Nishi
Department of Biological Chemistry Schol of Medicine, University of Davis, Davis, California 95616, USA
Exp Cell Res 280:233-43. 2002..The results indicate that the cell shape change caused by staurosporine correlates with the accumulation of p27kip1 and that staurosporine interferes with the p27kip1-specific proteolysis activity...
- Myosin light chain phosphorylation and growth cone motilityJohn T Schmidt
Department of Biological Sciences and Neuroscience Research Center, University at Albany SUNY, New York 12222, USA
J Neurobiol 52:175-88. 2002....
- Blebbistatin extends culture life of adult mouse cardiac myocytes and allows efficient and stable transgene expressionZhyldyz Kabaeva
Dept of Molecular and Integrative Physiology, University of Michigan, 7623A Medical Science II, Ann Arbor, MI 48109 0622, USA
Am J Physiol Heart Circ Physiol 294:H1667-74. 2008..Furthermore, the unwanted effects of BDM on adult mouse cardiac myocytes, perhaps due to its nonspecific activities or action as a chemical phosphatase, can be avoided by using blebbistatin...
- Differential localization of unconventional myosin I and nonmuscle myosin II during B cell spreadingAdriana Sumoza-Toledo
Department of Molecular Biomedicine, Centro de Investigacion y Estudios Avanzados, Av IPN 2508 Col Zacatenco CP 07360, Mexico, D F, Mexico
Exp Cell Res 312:3312-22. 2006..This work shows that class I and class II myosin are expressed in B cells, are differentially distributed, and may participate in the morphological changes of these cells...
- Effects of heart isolation, voltage-sensitive dye, and electromechanical uncoupling agents on ventricular fibrillationHao Qin
Department of Physiology and Biophysics, University of Alabama, 1670 University Boulevard, Birmingham, AL 35294, USA
Am J Physiol Heart Circ Physiol 284:H1818-26. 2003..dye di-4-ANEPPS, and six more episodes were mapped after exposure to the electromechanical uncoupling agents diacetyl monoxime (DAM; 20 mmol/l, n = 6) or cytochalasin D (CytoD; 10 micromol/l, n = 6)...
- Developmental reorientation of transverse cortical microtubules to longitudinal directions: a role for actomyosin-based streaming and partial microtubule-membrane detachmentFrank Sainsbury
School of Biological Sciences, University of Sydney, Sydney, NSW 2006, Australia
Plant J 56:116-31. 2008....
- Both Ca2+ -dependent and -independent pathways are involved in rat hepatic stellate cell contraction and intrahepatic hyperresponsiveness to methoxamineWim Laleman
Dept of Hepatology, Univ Hospital Gasthuisberg, K U Leuven, Leuven, Belgium
Am J Physiol Gastrointest Liver Physiol 292:G556-64. 2007....
- Spontaneous oscillation by hair bundles of the bullfrog's sacculusPascal Martin
Howard Hughes Medical Institute and Laboratory of Sensory Neuroscience, The Rockefeller University, New York, New York 10021 6399, USA
J Neurosci 23:4533-48. 2003..These observations accord with a model in which oscillations arise from the interplay of the hair bundle's negative stiffness with the activity of adaptation motors and with Ca 2+-dependent relaxation of gating springs...
- Contractile arrest reveals calcium-dependent stimulation of SERCA2a mRNA expression in cultured ventricular cardiomyocytesRonald Vlasblom
Laboratory for Physiology, Institute for Cardiovascular Research ICaR VU, VU University Medical Center, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands
Cardiovasc Res 63:537-44. 2004....
- Crucial role of cytoskeleton reorganization in the negative inotropic effect of chromogranin A-derived peptides in eel and frog heartsRosa Mazza
Department of Cell Biology, University of Calabria, 87030 Arcavacata di Rende Cs, Italy
Regul Pept 138:145-51. 2007..These results demonstrate that changes in cytoskeletal dynamics play a crucial role in the negative inotropic influence of VSs on eel and frog hearts...
- Hair-bundle movements elicited by transepithelial electrical stimulation of hair cells in the sacculus of the bullfrogD Bozovic
The Howard Hughes Medical Institute and Laboratory of Sensory Neuroscience, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
Proc Natl Acad Sci U S A 100:958-63. 2003..A model for electrically driven bundle motion that incorporates the negative stiffness of the hair bundle as well as its two mechanisms of motility captures the essential features of the measured responses...
- Beer spoilage bacteria and hop resistanceKanta Sakamoto
Fundamental Research Laboratory, Asahi Breweries, Ltd 1 21, Midori 1 chome, Moriya shi, Ibaraki 302 0106, Japan
Int J Food Microbiol 89:105-24. 2003..They can spoil beer by turbidity, acidity and the production of unfavorable smell such as diacetyl or hydrogen sulfide...
- Purification and characterization of acetoin:2,6-dichlorophenolindophenol oxidoreductase, dihydrolipoamide dehydrogenase, and dihydrolipoamide acetyltransferase of the Pelobacter carbinolicus acetoin dehydrogenase enzyme systemF B Oppermann
Institut für Mikrobiologie der Georg August Universität, Gottingen, Federal Republic of Germany
J Bacteriol 173:757-67. 1991..it catalyzed a thiamine pyrophosphate-dependent oxidative-hydrolytic cleavage of acetoin, methylacetoin, and diacetyl. The combination of purified Ao:DCPIP OR, DHLTA, and DHLDH in the presence of thiamine pyrophosphate and the ..
- Characterization of a (2R,3R)-2,3-butanediol dehydrogenase as the Saccharomyces cerevisiae YAL060W gene product. Disruption and induction of the geneE Gonzalez
Department of Biochemistry and Molecular Biology, Faculty of Sciences, Universitat Autonoma de Barcelona, E 08193 Bellaterra Barcelona, and Spain
J Biol Chem 275:35876-85. 2000..It was unable, however, to further oxidize these acetoins to diacetyl. In the presence of NADH, it could catalyze the stereospecific reduction of racemic acetoin ((3R/3S)- acetoin; K(..
- HYPOXIC IMAGING GUIDED INTENSITY MODULATED RADIOTHERAPYKS CLIFFORD CHAO; Fiscal Year: 2003..Cu(II)-diacetyl-bis(N4-methylthiosemicarbazone), or (Cu-ATSM), is a newly developed hypoxic marker for detecting tumor hypoxia ..
- Absorption, distribution, metabolism, excretion (ADME) of chemicals in mammalsRochelle W Tyl; Fiscal Year: 2009..identification;toxicokinetics;Mechanisms of toxicity;2,3-butanedione;diacetyl;N,N-dimethylacetoacetamide;obliterative bronchiolitis;CYP;eugenol;safrole;estragole;anethole;myristicin;..
- Research on inhalation toxicology of environmental chemicalsROBERT O apos CONNER; Fiscal Year: 2010..pentoxide;bleomycin, pulmonary function;ovalbumin, cigarette smoke, diacetyl, 2,3-pentanedione, benzene...
- Research on inhalation toxicology of environmental chemicalsROBERT O'CONNER; Fiscal Year: 2011..pentoxide;bleomycin, pulmonary function;ovalbumin, cigarette smoke, diacetyl, 2,3-pentanedione, benzene...
- Regulation of p53 by TAF1Xuan Liu; Fiscal Year: 2012..Upon DNA damage, K373/K382 diacetyl-p53 directly interacts with the double-bromodomain of TAF1 and recruits TAF1 to p21 promoter independent of other ..
- INTRA-TUMORAL METABOLIC HETEROGENEITY OF CERVICAL CANCERPERRY WAYNE GRIGSBY; Fiscal Year: 2013..with 3'-Deoxy-3'-18F-fluorothymidine (FLT) (marker of cellular proliferation) and PET with copper(II)-diacetyl-bis(N4-methylthiosemicarbazone) (Cu-ATSM) (marker of assessment of hypoxia) to identify areas of metabolic ..
- Conceptual Prediction of Drug Bioactivities in Cell-Based Assays: cell-QSARStefan Balaz; Fiscal Year: 2013..A novel surrogate solvent, diacetyl-PC (DAcPC, i.e...
- MINORITY PREDOCTORAL FELLOWSHIP PROGRAMNINITA BROWN; Fiscal Year: 2005..Uncoupling agents such as diacetyl monoxime (DAM) are used to inhibit mechanical contraction...
- Positron Emission Tomography in Prostate CancerFarrokh Dehdashti; Fiscal Year: 2008..PET may also contribute significant prognostic information that may affect the decision to administer early adjuvant therapy to delay or prevent recurrence. [unreadable] [unreadable]..
- DISTRIBUTED OCCUPATIONAL KNOWLEDGE SYSTEMPhilip Harber; Fiscal Year: 2001..The process also yields valuable surveillance data including population segments often missed by other methods and describing functional impact of work rather than just occupationally caused disease. ..
- Occupational Medicine Activities and Skills: An Empiric StudyPhilip Harber; Fiscal Year: 2009..Analyses will be both descriptive and address specific questions such as determining the impact of formal education and evaluating whether occupational preventive medicine is a homogeneous field or composed of distinct subgroups. ..
- REACTIVE INTERMEDIATES OF CARCINOGENESIS OF PAHSKENNETH LAALI; Fiscal Year: 2004..DNA binding and mammalian cell mutagenicity studies on the substituted and derivatized-PAHs proposed for stable ion work will enhance the available literature data on structure/reactivity relationships. ..
- CATCH: Mechanochemistry and Regulation in Smooth MuscleThomas Butler; Fiscal Year: 2008..4) Determination of the extent of involvement of myosin crossbridges in catch force maintenance by measurements of changes in orientation of fluorescent probes attached to the regulatory light chain. ..
- NEUROTOXIC SUPERFUND CHEMICALS AND BIOMAKERSPeter Spencer; Fiscal Year: 2007..abstract_text> ..
- Enteropathogenic E. coli Effects on Intestinal FunctionGail A Hecht; Fiscal Year: 2010..4. To evaluate the effect of EPEC infection on TJ structure and function in vivo using a mouse model and investigate the effector molecules and mechanisms involved. ..
- Fluorescence Lifetime and Steady-State SpectrometerE Ostap; Fiscal Year: 2003..The core user group has extensive training in the operation, design, and maintenance of spectroscopic equipment. ..
- Neurobiol mechanism/acupuncture therapy/alcoholismJiang Hong Ye; Fiscal Year: 2004..This new knowledge will have a major impact on our understanding of how acupuncture works. The application of this knowledge is expected to improve the effectiveness of acupuncture treatment in alcohol addiction. ..
- CORTICAL OSTEOPONTIN EXPRESSION IN HYDRONEPHROSISRUSSELL SCADUTO; Fiscal Year: 2002..In these studies the renin angiotensin system will be manipulated with the use of an ACE inhibitor, enalapril, and an AT-1 blocker, losartan. ..
- Two-Photon Absorption Imaging by Laser Pulse ShapingWarren Warren; Fiscal Year: 2004..abstract_text> ..
- Nasal dosimetry of diacetyl and butyric acid vaporsJohn Morris; Fiscal Year: 2005..Exposure to diacetyl (2,3 butadiene), a major component of butter flavoring vapors, results in necrosis of the nasal and trachea, but ..
- MECHANISM AND REGULATION OF ACTIN-BASED RETROGRADE FLOWJOHN HENSON; Fiscal Year: 2005..4. To study the inducible transformation of coelomocytes from a lamellipodial to a filopodial form. ..
- Functional assessment of donor-host cell couplingMICHAEL RUBART VAN DER LOHE; Fiscal Year: 2009..The studies proposed here will address fundamental hypotheses regarding the fate of donor cells following cellular transplantation into normal and injured hearts. ..
- Gustatory and olfactory receptors of Anopheles gambiaeHugh Robertson; Fiscal Year: 2006..This work will also contribute to our overall understanding of the molecular basis of chemoreception in insects and other animals. [unreadable] [unreadable]..
- Innate Immune Responses in Obliterative BronchiolitisScott Palmer; Fiscal Year: 2006..He will apply these skills to direct future investigations of patient oriented research problems in pulmonary medicine...
- ROS Defense Mechanisms in Brain ReoxygenationSarah Milton; Fiscal Year: 2004....
- ETHANOL EFFECTS ON GLYCINE RECEPTOR/CHANNEL FUNCTIONJiang Hong Ye; Fiscal Year: 2003....
- Intermolecular Multiple-Quantum Coherence Effects in NMRWarren Warren; Fiscal Year: 2002....
- MARINER TRANSPOSONS OF MAMMALSHugh Robertson; Fiscal Year: 2001..It will also reveal how occasionally particular copies of transposons can become integral parts of their host genetic complements. ..
- Study of Intrinsic Bladder Activity by Optical ImagingAnthony Kanai; Fiscal Year: 2007..To study the neural-chemical control of spontaneous detrusor activity; and III.) To determine the origin of detrusor overactivity in partially obstructed bladders. ..
- Ethanol and mesolimbic GABAergic neuronsJiang Hong Ye; Fiscal Year: 2007..Such results will provide valuable information on novel mechanisms underlying the addictive properties of alcohol and a firm scientific foundation for future neurobiological studies. [unreadable] [unreadable] [unreadable]..