proline

Summary

Summary: A non-essential amino acid that is synthesized from GLUTAMIC ACID. It is an essential component of COLLAGEN and is important for proper functioning of joints and tendons.

Top Publications

  1. pmc Boceprevir for previously treated chronic HCV genotype 1 infection
    Bruce R Bacon
    Saint Louis University School of Medicine, St Louis, Mo 63110 0250, USA
    N Engl J Med 364:1207-17. 2011
  2. doi Proline: a multifunctional amino acid
    Laszlo Szabados
    Institute of Plant Biology, Biological Research Center, Temesvari krt 62, H 6726 Szeged, Hungary
    Trends Plant Sci 15:89-97. 2010
  3. ncbi Duplicated P5CS genes of Arabidopsis play distinct roles in stress regulation and developmental control of proline biosynthesis
    Gyongyi Szekely
    Institute of Plant Biology, Biological Research Center, H 6726 Szeged, Hungary
    Plant J 53:11-28. 2008
  4. doi EF-P is essential for rapid synthesis of proteins containing consecutive proline residues
    Lili K Doerfel
    Department of Physical Biochemistry, Max Planck Institute for Biophysical Chemistry, Goettingen, Germany
    Science 339:85-8. 2013
  5. ncbi Homer binds a novel proline-rich motif and links group 1 metabotropic glutamate receptors with IP3 receptors
    J C Tu
    Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Neuron 21:717-26. 1998
  6. doi Efficacy of boceprevir, an NS3 protease inhibitor, in combination with peginterferon alfa-2b and ribavirin in treatment-naive patients with genotype 1 hepatitis C infection (SPRINT-1): an open-label, randomised, multicentre phase 2 trial
    Paul Y Kwo
    Department of Medicine, Division of Gastroenterology Hepatology, Indiana University School of Medicine, Indianapolis, IN 46202 5121, USA
    Lancet 376:705-16. 2010
  7. pmc Enhancing Arabidopsis salt and drought stress tolerance by chemical priming for its abscisic acid responses
    Gabor Jakab
    Institute of Botany, Biochemistry, University of Neuchatel, Switzerland
    Plant Physiol 139:267-74. 2005
  8. ncbi Mechanisms of high salinity tolerance in plants
    Narendra Tuteja
    Plant Molecular Biology, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India
    Methods Enzymol 428:419-38. 2007
  9. doi Proline metabolism and transport in plant development
    Silke Lehmann
    Institute of Plant Sciences, University of Bern, Switzerland
    Amino Acids 39:949-62. 2010
  10. pmc A proline-rich region in the coxsackievirus 3A protein is required for the protein to inhibit endoplasmic reticulum-to-golgi transport
    Els Wessels
    Department of Medical Microbiology, Nijmegen Center for Molecular Life Sciences, University Medical Center Nijmegen, P O Box 9101, 6500 HB Nijmegen, The Netherlands
    J Virol 79:5163-73. 2005

Detail Information

Publications356 found, 100 shown here

  1. pmc Boceprevir for previously treated chronic HCV genotype 1 infection
    Bruce R Bacon
    Saint Louis University School of Medicine, St Louis, Mo 63110 0250, USA
    N Engl J Med 364:1207-17. 2011
    ..Boceprevir, a protease inhibitor that binds to the HCV nonstructural 3 (NS3) active site, has been suggested as an additional treatment...
  2. doi Proline: a multifunctional amino acid
    Laszlo Szabados
    Institute of Plant Biology, Biological Research Center, Temesvari krt 62, H 6726 Szeged, Hungary
    Trends Plant Sci 15:89-97. 2010
    b>Proline accumulates in many plant species in response to environmental stress. Although much is now known about proline metabolism, some aspects of its biological functions are still unclear...
  3. ncbi Duplicated P5CS genes of Arabidopsis play distinct roles in stress regulation and developmental control of proline biosynthesis
    Gyongyi Szekely
    Institute of Plant Biology, Biological Research Center, H 6726 Szeged, Hungary
    Plant J 53:11-28. 2008
    Delta-1-pyrroline-5-carboxylate synthetase enzymes, which catalyse the rate-limiting step of proline biosynthesis, are encoded by two closely related P5CS genes in Arabidopsis...
  4. doi EF-P is essential for rapid synthesis of proteins containing consecutive proline residues
    Lili K Doerfel
    Department of Physical Biochemistry, Max Planck Institute for Biophysical Chemistry, Goettingen, Germany
    Science 339:85-8. 2013
    ..ribosome from stalling during synthesis of proteins containing consecutive prolines, such as PPG, PPP, or longer proline strings, in natural and engineered model proteins...
  5. ncbi Homer binds a novel proline-rich motif and links group 1 metabotropic glutamate receptors with IP3 receptors
    J C Tu
    Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Neuron 21:717-26. 1998
    ..A novel proline-rich "Homer ligand" (PPXXFr) is identified in group 1 mGluRs and IP3R, and these receptors coimmunoprecipitate as ..
  6. doi Efficacy of boceprevir, an NS3 protease inhibitor, in combination with peginterferon alfa-2b and ribavirin in treatment-naive patients with genotype 1 hepatitis C infection (SPRINT-1): an open-label, randomised, multicentre phase 2 trial
    Paul Y Kwo
    Department of Medicine, Division of Gastroenterology Hepatology, Indiana University School of Medicine, Indianapolis, IN 46202 5121, USA
    Lancet 376:705-16. 2010
    ..We tested the efficacy of boceprevir, an NS3 hepatitis C virus oral protease inhibitor, when added to peginterferon alfa-2b and ribavirin...
  7. pmc Enhancing Arabidopsis salt and drought stress tolerance by chemical priming for its abscisic acid responses
    Gabor Jakab
    Institute of Botany, Biochemistry, University of Neuchatel, Switzerland
    Plant Physiol 139:267-74. 2005
    ..Here, we show a possibility to increase plant tolerance for these abiotic stresses through effective priming of the preexisting defense pathways without resorting to genetic alterations...
  8. ncbi Mechanisms of high salinity tolerance in plants
    Narendra Tuteja
    Plant Molecular Biology, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India
    Methods Enzymol 428:419-38. 2007
    ..ion pumps, calcium, SOS pathways, ABA, transcription factors, mitogen-activated protein kinases, glycine betaine, proline, reactive oxygen species, and DEAD-box helicases in salinity stress tolerance...
  9. doi Proline metabolism and transport in plant development
    Silke Lehmann
    Institute of Plant Sciences, University of Bern, Switzerland
    Amino Acids 39:949-62. 2010
    b>Proline fulfils diverse functions in plants. As amino acid it is a structural component of proteins, but it also plays a role as compatible solute under environmental stress conditions...
  10. pmc A proline-rich region in the coxsackievirus 3A protein is required for the protein to inhibit endoplasmic reticulum-to-golgi transport
    Els Wessels
    Department of Medical Microbiology, Nijmegen Center for Molecular Life Sciences, University Medical Center Nijmegen, P O Box 9101, 6500 HB Nijmegen, The Netherlands
    J Virol 79:5163-73. 2005
    ..CVB 3A contains a conserved proline-rich region in its N terminus...
  11. pmc Critical roles of phosphorylation and actin binding motifs, but not the central proline-rich region, for Ena/vasodilator-stimulated phosphoprotein (VASP) function during cell migration
    Joseph J Loureiro
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 4307, USA
    Mol Biol Cell 13:2533-46. 2002
    ..Interestingly, expression of the C-terminal EVH2 domain alone is sufficient to complement loss of Ena/VASP function in random cell motility...
  12. pmc Independent function of two destruction domains in hypoxia-inducible factor-alpha chains activated by prolyl hydroxylation
    N Masson
    The Henry Wellcome Building of Genomic Medicine, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK
    EMBO J 20:5197-206. 2001
    ..The definition of two independent destruction domains implicates a more complex system of pVHL-HIF-alpha interactions, but reinforces the role of prolyl hydroxylation as an oxygen-dependent destruction signal...
  13. ncbi Stress-induced synthesis of proline confers tolerance to water deficit in transgenic wheat
    Eliane Cristina Gruszka Vendruscolo
    Universidade Federal do Paraná Campus Palotina, Rua Pioneiro, 2153, CEP 85950 000 Palotina Pr, Brazil
    J Plant Physiol 164:1367-76. 2007
    ..Accumulation of proline appears to be a promising approach to maintain the productivity of plants under stress condition...
  14. ncbi Folding of helical membrane proteins: the role of polar, GxxxG-like and proline motifs
    Alessandro Senes
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6059, USA
    Curr Opin Struct Biol 14:465-79. 2004
    ..Three types of transmembrane motifs have recently seen interesting developments: the GxxxG motif and its like; polar and hydrogen bonding motifs; and proline motifs.
  15. pmc Proline-rich (PxxP) motifs in HIV-1 Nef bind to SH3 domains of a subset of Src kinases and are required for the enhanced growth of Nef+ viruses but not for down-regulation of CD4
    K Saksela
    Rockefeller University, New York, NY 10021
    EMBO J 14:484-91. 1995
    ..A unique cooperative role of a distant proline is also observed...
  16. doi Proline accumulation in plants: a review
    Nathalie Verbruggen
    Laboratoire de Physiologie et de Génétique Moléculaire des Plantes, Universite Libre de Bruxelles, Campus Plaine CP242, Bd du Triomphe, 1050, Brussels, Belgium
    Amino Acids 35:753-9. 2008
    b>Proline (Pro) accumulation is a common physiological response in many plants in response to a wide range of biotic and abiotic stresses. Controversy has surrounded the possible role(s) of proline accumulation...
  17. ncbi Proline cis-trans isomerization and protein folding
    William J Wedemeyer
    Baker Laboratory of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853 1301, USA
    Biochemistry 41:14637-44. 2002
    b>Proline cis-trans isomerization plays a key role in the rate-determining steps of protein folding...
  18. ncbi The importance of being proline: the interaction of proline-rich motifs in signaling proteins with their cognate domains
    B K Kay
    Department of Pharmacology, University of Wisconsin Madison, Madison, Wisconsin 53706 1532, USA
    FASEB J 14:231-41. 2000
    ..Among the primary structures of many ligands for protein-protein interactions, the amino acid proline is critical...
  19. ncbi Does proline accumulation play an active role in stress-induced growth reduction?
    Albino Maggio
    Center for Plant Environmental Stress Physiology, Purdue University, 1165 Horticulture Bldg, West Lafayette, Indiana 47907 1165, USA
    Plant J 31:699-712. 2002
    ..To examine this possibility more closely, intracellular proline level was manipulated by expressing mutated derivatives of tomPRO2 (a Delta(1)-pyrroline-5-carboxylate synthetase,..
  20. ncbi Identification of a proline-rich Akt substrate as a 14-3-3 binding partner
    Kristina S Kovacina
    Department of Molecular Pharmacology, Stanford University, California 94305 5174, USA
    J Biol Chem 278:10189-94. 2003
    ..This 40-kDa protein, designated PRAS40, is a proline-rich Akt substrate...
  21. pmc Reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses regulated by oncogenic transcription factor c-MYC
    Wei Liu
    Metabolism and Cancer Susceptibility Section, Basic Research Laboratory, National Cancer Institute, Frederick, MD 21702, USA
    Proc Natl Acad Sci U S A 109:8983-8. 2012
    ..Less well-recognized, glutamate can also be converted to proline through Δ(1)-pyrroline-5-carboxylate (P5C) and vice versa...
  22. ncbi Prolyl cis-trans isomerization as a molecular timer
    Kun Ping Lu
    Cancer Biology Program, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 77 Avenue Louis Pasteur, NRB 1030, Boston, Massachusetts 02215, USA
    Nat Chem Biol 3:619-29. 2007
    b>Proline is unique in the realm of amino acids in its ability to adopt completely distinct cis and trans conformations, which allows it to act as a backbone switch that is controlled by prolyl cis-trans isomerization...
  23. pmc Impaired insulin/IGF1 signaling extends life span by promoting mitochondrial L-proline catabolism to induce a transient ROS signal
    Kim Zarse
    Department of Human Nutrition, Institute of Nutrition, University of Jena, Jena, Germany
    Cell Metab 15:451-65. 2012
    ..IIIS upregulates mitochondrial L-proline catabolism, and impairment of the latter impairs the life span-extending capacity of iIIS while L-proline ..
  24. doi Loss of function of a proline-containing protein confers durable disease resistance in rice
    Shuichi Fukuoka
    QTL Genomics Research Center, National Institute of Agrobiological Sciences, Kannondai 2 1 2, Tsukuba, Ibaraki 305 8602, Japan
    Science 325:998-1001. 2009
    ..Pi21 encodes a proline-rich protein that includes a putative heavy metal-binding domain and putative protein-protein interaction motifs...
  25. ncbi Sequence-specific and phosphorylation-dependent proline isomerization: a potential mitotic regulatory mechanism
    M B Yaffe
    Department of Medicine Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    Science 278:1957-60. 1997
    ..Pin1 is here shown to be a phosphorylation-dependent PPIase that specifically recognizes the phosphoserine-proline or phosphothreonine-proline bonds present in mitotic phosphoproteins...
  26. ncbi The common PPARgamma Pro12Ala polymorphism is associated with decreased risk of type 2 diabetes
    D Altshuler
    Whitehead Institute MIT Center for Genome Research, Cambridge, Massachusetts, USA
    Nat Genet 26:76-80. 2000
    ..25-fold) but significant (P=0.002) increase in diabetes risk associated with the more common proline allele (85% frequency). Moreover, our results resolve a controversy about common variation in PPARgamma...
  27. pmc Proline dehydrogenase is essential for proline protection against hydrogen peroxide-induced cell death
    Sathish Kumar Natarajan
    Department of Biochemistry and Redox Biology Center, University of Nebraska Lincoln, Lincoln, NE 68588, USA
    Free Radic Biol Med 53:1181-91. 2012
    b>Proline metabolism has an underlying role in apoptotic signaling that influences tumorigenesis. Proline is oxidized to glutamate in the mitochondria, with the rate-limiting step catalyzed by proline dehydrogenase (PRODH)...
  28. pmc A novel proteolytic cascade generates an extracellular matrix-derived chemoattractant in chronic neutrophilic inflammation
    Amit Gaggar
    Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Immunol 180:5662-9. 2008
    ..In this study, we demonstrate that a novel extracellular matrix-derived neutrophil chemoattractant, proline-glycine-proline (PGP), acts through CXC receptors 1 and 2 on neutrophils, similar to N-acetylated proline-glycine-..
  29. ncbi A novel proline-rich protein, EspF, is secreted from enteropathogenic Escherichia coli via the type III export pathway
    B P McNamara
    Department of Medicine, University of Maryland School of Medicine, Baltimore 21201, USA
    FEMS Microbiol Lett 166:71-8. 1998
    ..In this study, we describe a proline-rich protein, EspF, encoded by the LEE that is secreted by the EPEC type III secretion apparatus...
  30. doi The proline biosynthetic genes P5CS1 and P5CS2 play overlapping roles in Arabidopsis flower transition but not in embryo development
    Roberto Mattioli
    Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Genetica e Biologia Molecolare, Universita di Roma La Sapienza, P le Aldo Moro 5, 00185, Roma, Italy
    Physiol Plant 137:72-85. 2009
    Overexpression of the proline biosynthetic gene P5CS1 results in early flowering in Arabidopsis...
  31. doi Regulation of proline metabolism in mycobacteria and its role in carbon metabolism under hypoxia
    Michael Berney
    Department of Microbiology and Immunology, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand
    Mol Microbiol 84:664-81. 2012
    Genes with a role in proline metabolism are strongly expressed when mycobacterial cells are exposed to nutrient starvation and hypoxia...
  32. pmc Osmotic control of opuA expression in Bacillus subtilis and its modulation in response to intracellular glycine betaine and proline pools
    Tamara Hoffmann
    Philipps University Marburg, Department of Biology, Laboratory for Microbiology, Marburg, Germany
    J Bacteriol 195:510-22. 2013
    ..The amassing of glycine betaine caused repression of the formation of an osmostress-adaptive pool of proline, the only osmoprotectant that B. subtilis can synthesize de novo...
  33. pmc Purification and characterization of Put1p from Saccharomyces cerevisiae
    Srimevan Wanduragala
    Department of Biochemistry, University of Nebraska Lincoln, Lincoln, NE 68588, USA
    Arch Biochem Biophys 498:136-42. 2010
    In Saccharomyces cerevisiae, the PUT1 and PUT2 genes are required for the conversion of proline to glutamate. The PUT1 gene encodes Put1p, a proline dehydrogenase (PRODH) enzyme localized in the mitochondrion...
  34. pmc Intron-mediated alternative splicing of Arabidopsis P5CS1 and its association with natural variation in proline and climate adaptation
    Ravi Kesari
    Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan
    Proc Natl Acad Sci U S A 109:9197-202. 2012
    Drought-induced proline accumulation is widely observed in plants but its regulation and adaptive value are not as well understood...
  35. pmc Structural and energetic basis of ALS-causing mutations in the atypical proline-tyrosine nuclear localization signal of the Fused in Sarcoma protein (FUS)
    Zi Chao Zhang
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 109:12017-21. 2012
    Mutations in the proline/tyrosine-nuclear localization signal (PY-NLS) of the Fused in Sarcoma protein (FUS) cause amyotrophic lateral sclerosis (ALS)...
  36. doi Detection and diversity of a putative novel heterogeneous polymorphic proline-glycine repeat (Pgr) protein in the footrot pathogen Dichelobacter nodosus
    Leo A Calvo-Bado
    Department of Biological Sciences, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK
    Vet Microbiol 147:358-66. 2011
    ..The aim of this study was to determine the diversity of the pgr gene, which encodes a putative proline-glycine repeat protein (Pgr). The pgr gene was present in all 100 isolates of D...
  37. ncbi Regulatory role of arginase I and II in nitric oxide, polyamine, and proline syntheses in endothelial cells
    H Li
    Department of Animal Science, Texas A and M University, College Station, Texas 77843, USA
    Am J Physiol Endocrinol Metab 280:E75-82. 2001
    ..Basal nitric oxide (NO) production was reduced by 60% in AI-EC and by 47% in AII-EC. Glutamate and proline production from arginine increased by 164 and 928% in AI-EC and by 79 and 295% in AII-EC, respectively, compared ..
  38. doi Tobacco leaves and roots differ in the expression of proline metabolism-related genes in the course of drought stress and subsequent recovery
    Jana Dobra
    Institute of Experimental Botany, v v i, Academy of Sciences of the Czech Republic, Rozvojova 263, 165 02 Prague 6, Lysolaje, Czech Republic
    J Plant Physiol 168:1588-97. 2011
    ..Genes encoding proline dehydrogenase (PDH), the key enzyme in proline degradation, and the proline biosynthetic enzyme, Δ(1)-pyrroline-..
  39. pmc Deletion of v7-3 (SLC6A15) transporter allows assessment of its roles in synaptosomal proline uptake, leucine uptake and behaviors
    Jana Drgonova
    Molecular Neurobiology Branch, NIDA, NIH, 333 Cassell Drive, Baltimore, MD 21224, USA
    Brain Res 1183:10-20. 2007
    ..While our studies were in progress, v7-3 expression was reported to confer transport of proline and branched-chain amino acids in in vitro expression systems [Takanaga, H., Mackenzie, B., Peng, J.B...
  40. doi Deficiency in plastidic glutamine synthetase alters proline metabolism and transcriptomic response in Lotus japonicus under drought stress
    Pedro Diaz
    Departamento de Bioquimica Vegetal y Biologia Molecular, Facultad de Quimica, Universidad de Sevilla, Calle Profesor García González 1, 41012 Sevilla, Spain
    New Phytol 188:1001-13. 2010
    The role of plastidic glutamine synthetase (GS2) in proline biosynthesis and drought stress responses in Lotus japonicus was investigated using the GS2 mutant, Ljgln2-2...
  41. pmc Essential role of tissue-specific proline synthesis and catabolism in growth and redox balance at low water potential
    Sandeep Sharma
    Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan
    Plant Physiol 157:292-304. 2011
    To better define the still unclear role of proline (Pro) metabolism in drought resistance, we analyzed Arabidopsis (Arabidopsis thaliana) Δ(1)-pyrroline-5-carboxylate synthetase1 (p5cs1) mutants deficient in stress-induced Pro synthesis ..
  42. ncbi Cleavage N-terminal to proline: analysis of a database of peptide tandem mass spectra
    Linda A Breci
    Department of Chemistry, University of Arizona, Tucson 85721, USA
    Anal Chem 75:1963-71. 2003
    ....
  43. ncbi Modulation of intracellular proline levels affects flowering time and inflorescence architecture in Arabidopsis
    Roberto Mattioli
    Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Genetica e Biologia Molecolare, Universita di Roma La Sapienza, P le Aldo Moro 5, Rome 00185, Italy
    Plant Mol Biol 66:277-88. 2008
    ..in Nicotiana tabacum, and encodes ornithine cyclodeaminase, an enzyme catalysing the conversion of ornithine to proline. To investigate on the possible role of proline in flowering, we altered the expression of AtP5CS1, encoding the ..
  44. pmc Structural biology of proline catabolism
    John J Tanner
    Departments of Chemistry and Biochemistry, University of Missouri, Columbia, MO 65211, USA
    Amino Acids 35:719-30. 2008
    The proline catabolic enzymes proline dehydrogenase and Delta(1)-pyrroline-5-carboxylate dehydrogenase catalyze the 4-electron oxidation of proline to glutamate...
  45. pmc Proline modulates the intracellular redox environment and protects mammalian cells against oxidative stress
    Navasona Krishnan
    Department of Biochemistry, University of Nebraska Lincoln, Lincoln, NE 68588, USA
    Free Radic Biol Med 44:671-81. 2008
    The potential of proline to suppress reactive oxygen species (ROS) and apoptosis in mammalian cells was tested by manipulating intracellular proline levels exogenously and endogenously by overexpression of proline metabolic enzymes...
  46. pmc Slow peptide bond formation by proline and other N-alkylamino acids in translation
    Michael Y Pavlov
    Department of Cell and Molecular Biology, Uppsala University, BMC, Box 596, 751 24 Uppsala, Sweden
    Proc Natl Acad Sci U S A 106:50-4. 2009
    Proteins are made from 19 aa and, curiously, one N-alkylamino acid ("imino acid"), proline (Pro)...
  47. ncbi Evidence that differential gene expression between the halophyte, Thellungiella halophila, and Arabidopsis thaliana is responsible for higher levels of the compatible osmolyte proline and tight control of Na+ uptake in T. halophila
    Surya Kant
    Albert Katz Department of Dryland Biotechnologies, Jacob Blaustein Institutes for Desert Research, Ben Gurion University of the Negev, Midreshet Ben Gurion 84990, Israel
    Plant Cell Environ 29:1220-34. 2006
    ..thaliana. Thellungiella halophila contained higher unstressed levels of the compatible osmolyte proline than A. thaliana, while under salt stress, T. halophila accumulated more proline mainly in shoots...
  48. pmc HIF-1alpha binding to VHL is regulated by stimulus-sensitive proline hydroxylation
    F Yu
    Department of Pathology and Laboratory Medicine, University of Pennsylvania Cancer Center, University of Pennsylvania School of Medicine, Philadelphia 19104, USA
    Proc Natl Acad Sci U S A 98:9630-5. 2001
    ..The data furthermore show that this proline hydroxylation is the primary regulator of VHL binding.
  49. ncbi WW and SH3 domains, two different scaffolds to recognize proline-rich ligands
    Maria J Macias
    Structural and Computational Biology Program, EMBL Heidelberg, Heidelberg, Germany
    FEBS Lett 513:30-7. 2002
    ..These domains fold as a stable, triple stranded beta-sheet and recognize proline-containing ligands...
  50. ncbi Effect of experimental elevation of free fatty acids on insulin secretion and insulin sensitivity in healthy carriers of the Pro12Ala polymorphism of the peroxisome proliferator--activated receptor-gamma2 gene
    N Stefan
    Medizinische Klinik, Abteilung für Endokrinologie, Stoffwechsel und Pathobiochemie, Eberhard Karls Universitat, Tubingen, Germany
    Diabetes 50:1143-8. 2001
    ..8). In conclusion, these results provide evidence that the Pro12Ala polymorphism in the PPAR-gamma2 gene might be involved in a differential regulation of insulin secretion in response to increased FFAs in humans...
  51. ncbi Point mutations in the proline-rich region of p22phox are dominant inhibitors of Nox1- and Nox2-dependent reactive oxygen generation
    Tsukasa Kawahara
    Department of Pathology and Experimental Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 280:31859-69. 2005
    ..p22phox associates with gp91phox and, through its proline-rich C terminus, provides a binding site for the tandem Src homology 3 domains of the activating subunit p47phox...
  52. pmc Mitochondrial adaptations to NaCl. Complex I is protected by anti-oxidants and small heat shock proteins, whereas complex II is protected by proline and betaine
    E W Hamilton
    Department of Biology, Syracuse University, 130 College Place, Syracuse, New York 13244 1220, USA
    Plant Physiol 126:1266-74. 2001
    ..NaCl stress on maize (Zea mays) mitochondrial electron transport and its relative protection by osmoprotectants (proline, betaine, and sucrose), antioxidants (ascorbate, glutathione, and alpha-tocopherol), antioxidant enzymes (..
  53. ncbi A generic mechanism of beta2-microglobulin amyloid assembly at neutral pH involving a specific proline switch
    Timo Eichner
    Astbury Centre for Structural Molecular Biology and Institute of Molecular Cellular Biology University of Leeds, Leeds LS2 9JT, UK
    J Mol Biol 386:1312-26. 2009
    ..Here, we have used site-directed mutagenesis to create five proline to glycine variants in the naturally amyloidogenic protein beta2-microglobulin (beta2m)...
  54. pmc Structure and kinetics of monofunctional proline dehydrogenase from Thermus thermophilus
    Tommi A White
    Department of Biochemistry, University of Missouri Columbia, Columbia, MO 65211, USA
    J Biol Chem 282:14316-27. 2007
    b>Proline dehydrogenase (PRODH) and Delta(1)-pyrroline-5-carboxylate dehydrogenase (P5CDH) catalyze the two-step oxidation of proline to glutamate...
  55. ncbi Yes-associated protein (YAP65) is a proline-rich phosphoprotein that binds to the SH3 domain of the Yes proto-oncogene product
    M Sudol
    Laboratory of Molecular Oncology, Rockefeller University, New York, NY 10021
    Oncogene 9:2145-52. 1994
    ..is of 65 kiloDalton (kDa) molecular mass, it is phosphorylated in vivo on serine and is particularly rich in proline. We named it YAP65 for Yes-Associated Protein of 65 kDa...
  56. ncbi Proline isomerization of histone H3 regulates lysine methylation and gene expression
    Christopher J Nelson
    Gurdon Institute and Department of Pathology, Tennis Court Road, Cambridge, CB2 1QR, UK
    Cell 126:905-16. 2006
    The cis-trans isomerization of proline serves as a regulatory switch in signaling pathways...
  57. doi Proline-rich antimicrobial peptides: converging to a non-lytic mechanism of action
    Marco Scocchi
    Dipartimento di Scienze della Vita, Universita di Trieste, Via Giorgieri 1, 34127, Trieste, Italy
    Cell Mol Life Sci 68:2317-30. 2011
    b>Proline-rich antimicrobial peptides are a group of cationic host defense peptides of vertebrates and invertebrates characterized by a high content of proline residues, often associated with arginine residues in repeated motifs...
  58. ncbi Overexpression of proline oxidase induces proline-dependent and mitochondria-mediated apoptosis
    Chien an A Hu
    Department of Biochemistry and Molecular Biology, University of New Mexico School of Medicine, Albuquerque, NM, USA
    Mol Cell Biochem 295:85-92. 2007
    b>Proline oxidase (POX), a mitochondrial inner-membrane protein, catalyzes the rate-limiting oxidation of proline to pyrroline- 5-carboxylate (P5C)...
  59. pmc The modified wobble nucleoside uridine-5-oxyacetic acid in tRNAPro(cmo5UGG) promotes reading of all four proline codons in vivo
    S Joakim Nasvall
    Department of Molecular Biology, Umea University, S 90 187 Umeå, Sweden
    RNA 10:1662-73. 2004
    ..In the proline family codon box, one (tRNAProcmo5UGG) of the three tRNAs that reads the four proline codons has cmo5U34...
  60. ncbi Allosteric regulation of Hsp70 chaperones by a proline switch
    Markus Vogel
    Zentrum für Molekulare Biologie der Universität Heidelberg ZMBI, Heidelberg, Germany
    Mol Cell 21:359-67. 2006
    ..Here we provide evidence that a universally conserved proline in the ATPase domain constitutes the switch that assumes alternate conformations in response to ATP binding and ..
  61. ncbi Pinning down proline-directed phosphorylation signaling
    Kun Ping Lu
    Cancer Biology Program, Division of Hematology Oncology, Dept of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, HIM 1047, Boston, MA 02215, USA
    Trends Cell Biol 12:164-72. 2002
    The reversible phosphorylation of proteins on serine or threonine residues preceding proline (Ser/Thr-Pro) is a major cellular signaling mechanism...
  62. pmc The WW domain of Yes-associated protein binds a proline-rich ligand that differs from the consensus established for Src homology 3-binding modules
    H I Chen
    Laboratory of Molecular Oncology, Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 92:7819-23. 1995
    ..Peptide sequence comparison between the two partial clones revealed a homologous region consisting of a proline-rich domain followed by a tyrosine residue (with the shared sequence PPPPY), which we shall call the PY motif...
  63. ncbi Proline and glycine control protein self-organization into elastomeric or amyloid fibrils
    Sarah Rauscher
    Molecular Structure and Function Programme, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada
    Structure 14:1667-76. 2006
    ..including those of elastin, amyloids, spider silks, wheat gluten, and insect resilin, reveals a threshold in proline and glycine composition above which amyloid formation is impeded and elastomeric properties become apparent...
  64. pmc A critical role for LTA4H in limiting chronic pulmonary neutrophilic inflammation
    Robert J Snelgrove
    Division of Pulmonary, Allergy and Critical Care Medicine, University of Alabama at Birmingham Lung Health Center, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Science 330:90-4. 2010
    ..activity with unknown substrate and physiological importance; we identified the neutrophil chemoattractant proline-glycine-proline (PGP) as this physiological substrate...
  65. ncbi Correlation between the induction of a gene for delta 1-pyrroline-5-carboxylate synthetase and the accumulation of proline in Arabidopsis thaliana under osmotic stress
    Y Yoshiba
    Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research RIKEN, Ibaraki, Japan
    Plant J 7:751-60. 1995
    ..a cDNA for delta 1-pyrroline-5-carboxylate (P5C) synthetase (cAtP5CS), an enzyme involved in the biosynthesis of proline, from a cDNA library prepared from a dehydrated rosette plant of Arabidopsis thaliana...
  66. pmc Proline antagonizes GABA-induced quenching of quorum-sensing in Agrobacterium tumefaciens
    E Haudecoeur
    Centre National de la Recherche Scientifique, Institut des Sciences du Vegetal, Gif sur Yvette 91 198, France
    Proc Natl Acad Sci U S A 106:14587-92. 2009
    Plants accumulate free L-proline (Pro) in response to abiotic stresses (drought and salinity) and presence of bacterial pathogens, including the tumor-inducing bacterium Agrobacterium tumefaciens...
  67. doi Proline as a stress protectant in yeast: physiological functions, metabolic regulations, and biotechnological applications
    Hiroshi Takagi
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara, 630 0192, Japan
    Appl Microbiol Biotechnol 81:211-23. 2008
    b>Proline is an important amino acid in terms of its biological functions and biotechnological applications. In response to osmotic stress, proline is accumulated in many bacterial and plant cells as an osmoprotectant...
  68. doi The metabolism of proline, a stress substrate, modulates carcinogenic pathways
    James M Phang
    Laboratory of Comparative Carcinogenesis, Center for Cancer Research, Building 538, Room 115, NCI Frederick, Frederick, MD 21702, USA
    Amino Acids 35:681-90. 2008
    The resurgence of interest in tumor metabolism has led investigators to emphasize the metabolism of proline as a "stress substrate" and to suggest this pathway as a potential anti-tumor target. Proline oxidase, a.k.a...
  69. pmc Conformation of a group 2 late embryogenesis abundant protein from soybean. Evidence of poly (L-proline)-type II structure
    Jose L Soulages
    Department of Biochemistry and Molecular Biology, 355 Noble Research Center, Oklahoma State University, Stillwater, Oklahoma 74078 0454, USA
    Plant Physiol 131:963-75. 2003
    ..in equilibrium between two extended conformational states: unordered and left-handed extended helical or poly (L-proline)-type II structures...
  70. doi Enhancing the thermostability of alpha-glucosidase from Thermoanaerobacter tengcongensis MB4 by single proline substitution
    Cheng Zhou
    State Key Lab of Microbial Resources, Institute of Microbiology, Chinese Academy of Science, Beijing 100101, China
    J Biosci Bioeng 110:12-7. 2010
    ..on the amino acid sequence alignment, four sites (Leu152, Asn208, Lys285, and Thr430) of TtGluA were chosen for proline substitution to improve its thermostability...
  71. pmc Actions of a proline analogue, L-thiazolidine-4-carboxylic acid (T4C), on Trypanosoma cruzi
    Anahi Magdaleno
    Departamento de Parasitologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, Brazil
    PLoS ONE 4:e4534. 2009
    It is well established that L-proline has several roles in the biology of trypanosomatids...
  72. doi Evidence that biosynthesis of the neurotoxic alkaloids anatoxin-a and homoanatoxin-a in the cyanobacterium Oscillatoria PCC 6506 occurs on a modular polyketide synthase initiated by L-proline
    Annick Méjean
    Laboratoire de Biochimie des Microorganismes Enzymologie, Métabolisme et Antibiotiques UMR CNRS 7223, ENSCP, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
    J Am Chem Soc 131:7512-3. 2009
    ..b>Proline is first loaded on an acyl carrier protein and its five-membered cycle oxidized to the pyrroline oxidation state...
  73. pmc Proline substitutions and threonine pseudophosphorylation of the SH3 ligand of 18.5-kDa myelin basic protein decrease its affinity for the Fyn-SH3 domain and alter process development and protein localization in oligodendrocytes
    Graham S T Smith
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada
    J Neurosci Res 90:28-47. 2012
    ..The classic 18.5-kDa MBP isoform has a proline-rich region comprising amino acids 92-99 (murine sequence -T(92)PRTPPPS(99)-) that contains a minimal SH3 ligand ..
  74. pmc Proline-dependent regulation of Clostridium difficile Stickland metabolism
    Laurent Bouillaut
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA, USA
    J Bacteriol 195:844-54. 2013
    ..Reduction of the electron acceptors proline and glycine requires the d-proline reductase (PR) and the glycine reductase (GR) enzyme complexes, respectively...
  75. ncbi Full-sized RanBPM cDNA encodes a protein possessing a long stretch of proline and glutamine within the N-terminal region, comprising a large protein complex
    H Nishitani
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
    Gene 272:25-33. 2001
    ..Thus, full-sized RanBPM cDNA encodes a long stretch of proline and glutamine residues in the N-terminal region. It comprises a protein complex of more than 670 kDa...
  76. doi Ornithine-delta-aminotransferase and proline dehydrogenase genes play a role in non-host disease resistance by regulating pyrroline-5-carboxylate metabolism-induced hypersensitive response
    Muthappa Senthil-Kumar
    Plant Biology Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Pkwy, Ardmore, Oklahoma 73402, USA
    Plant Cell Environ 35:1329-43. 2012
    ..b>Proline catabolism reactions, especially pyrroline-5-carboxylate (P5C) accumulation, are known to produce ROS and ..
  77. pmc Proline oxidase promotes tumor cell survival in hypoxic tumor microenvironments
    Wei Liu
    Metabolism and Cancer Susceptibility Section, Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, Maryland 21702, USA
    Cancer Res 72:3677-86. 2012
    b>Proline is a readily released stress substrate that can be metabolized by proline oxidase (POX) to generate either reactive oxygen species (ROS) to induce apoptosis or autophagy or ATP during times of nutrient stress...
  78. doi Structural determinants stabilizing helical distortions related to proline
    Julien Rey
    CNRS UMR 6214 INSERM U771, Universite d Angers, Faculte de Medecine, 3 rue Haute de Reculée, 49045 Angers, France
    J Struct Biol 171:266-76. 2010
    The distortions related to proline in a alpha-helix can be accommodated by different structural elements...
  79. pmc Serine phosphorylation and proline isomerization in RNAP II CTD control recruitment of Nrd1
    Karel Kubicek
    CEITEC Central European Institute of Technology, Masaryk University, Brno, 62500, Czech Republic
    Genes Dev 26:1891-6. 2012
    ..These findings underpin the interplay between covalent and noncovalent changes in the CTD structure that constitute the CTD code...
  80. ncbi Proline-induced distortions of transmembrane helices
    Frank S Cordes
    Laboratory of Molecular Biophysics, The Rex Richards Building, Department of Biochemistry, University of Oxford, South Parks Road, UK
    J Mol Biol 323:951-60. 2002
    b>Proline residues in the transmembrane (TM) alpha-helices of integral membrane proteins have long been suspected to play a key role for helix packing and signal transduction by inducing regions of helix distortion and/or dynamic ..
  81. ncbi The proline-rich, extensin-like receptor kinase-1 (PERK1) gene is rapidly induced by wounding
    Nancy F Silva
    Department of Botany, University of Toronto, Ontario, Canada
    Plant Mol Biol 50:667-85. 2002
    We report the isolation and characterization of PERKI (Proline Extensin-like Receptor Kinase 1), a novel plant RLK from Brassica napus that is predicted to consist of a proline-rich extracellular domain with sequence similarity to ..
  82. pmc Computational identification and characterization of novel genes from legumes
    Michelle A Graham
    Department of Plant Biology, University of Minnesota, St Paul, Minnesota 55108, USA
    Plant Physiol 135:1179-97. 2004
    ..Evolutionary analyses of the genomic sequences of several CCPs in M. truncatula suggest that this family has evolved by local duplications and divergent selection...
  83. doi The conformation and the aggregation kinetics of α-synuclein depend on the proline residues in its C-terminal region
    Jessika Meuvis
    Laboratory of Biomolecular Dynamics, Katholieke Universiteit Leuven, Celestijnenlaan 200G, Leuven, Belgium
    Biochemistry 49:9345-52. 2010
    ..The C-terminal domain of α-syn is characterized by the presence of 15 acidic amino acids and all five proline residues of the protein (P108, P117, P120, P128, and P138)...
  84. ncbi Enhancement of ornithine production in proline-supplemented Corynebacterium glutamicum by ornithine cyclodeaminase
    Soo Youn Lee
    Department of Bioprocess Engineering, Chonbuk National University, Jeonju 561 756, Korea
    J Microbiol Biotechnol 20:127-31. 2010
    ..In this study, Corynebacterium glutamicum and its derived mutants were used to demonstrate the relationship between proline, glutamate and ornithine. The maximum ornithine production was shown in the culture medium (3295...
  85. ncbi Overexpression of OsCOIN, a putative cold inducible zinc finger protein, increased tolerance to chilling, salt and drought, and enhanced proline level in rice
    Kaimao Liu
    Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Science, Beijing 100093, China
    Planta 226:1007-16. 2007
    ..Over-expression of OsCOIN in transgenic rice lines significantly enhanced their tolerance to cold, salt and drought, accompanied by an up-regulation of OsP5CS expression and an increase of cellular proline level.
  86. ncbi The refined crystal structure of Bacillus cereus oligo-1,6-glucosidase at 2.0 A resolution: structural characterization of proline-substitution sites for protein thermostabilization
    K Watanabe
    Department of Agricultural Chemistry, Kyoto Prefectural University, Shimogamo, Sakyo, Japan
    J Mol Biol 269:142-53. 1997
    ..The refined structure reveals the locations of 21 proline-substitution sites that are expected to be critical to protein thermostabilization from a sequence comparison ..
  87. pmc Proline accumulation is inhibitory to Arabidopsis seedlings during heat stress
    Wei Tao Lv
    Research Center for Molecular and Developmental Biology, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, People s Republic of China
    Plant Physiol 156:1921-33. 2011
    The effect of proline (Pro) accumulation on heat sensitivity was investigated using transgenic Arabidopsis (Arabidopsis thaliana) plants ectopically expressing the Δ(1)-pyrroline-5-carboxylate synthetase 1 gene (AtP5CS1) under the ..
  88. ncbi Regulation of levels of proline as an osmolyte in plants under water stress
    Y Yoshiba
    Advanced Research Laboratory, Hitachi Ltd, Saitama, Japan
    Plant Cell Physiol 38:1095-102. 1997
    Compatible osmolytes are potent osmoprotectants that play a role in counteracting the effects of osmotic stress. Proline (Pro) is one of the most common compatible osmolytes in water-stressed plants...
  89. ncbi The osmoprotectant proline betaine is a major substrate for the binding-protein-dependent transport system ProU of Escherichia coli K-12
    M Haardt
    University of Konstanz, Department of Biology, Federal Republic of Germany
    Mol Gen Genet 246:783-6. 1995
    ..Here we report that both ProP and ProU are involved in the transport of the potent osmoprotectant proline betaine. A set of isogenic E. coli strains carrying deletions in either the proP or proU loci was constructed...
  90. pmc Role of proline under changing environments: a review
    Shamsul Hayat
    Department of Botany and Microbiology, Faculty of Science, King Saudi University, Riyadh, Saudi Arabia
    Plant Signal Behav 7:1456-66. 2012
    ..A large body of data suggests a positive correlation between proline accumulation and plant stress...
  91. doi Interaction of proline, sugars, and anthocyanins during photosynthetic acclimation of Arabidopsis thaliana to drought stress
    Ilektra Sperdouli
    Department of Botany, School of Biology, Aristotle University of Thessaloniki, University Campus, 54124 Thessaloniki, Greece
    J Plant Physiol 169:577-85. 2012
    The relationships among photosynthetic acclimation, proline (Pro), soluble sugar (SS), and anthocyanin (An) accumulation in Arabidopsis thaliana leaves to the onset of drought stress (OnDS), mild (MiDS) and moderate drought stress (MoDS),..
  92. pmc Synthesis, release, and recapture of compatible solute proline by osmotically stressed Bacillus subtilis cells
    Tamara Hoffmann
    Philipps University Marburg, Department of Biology, Laboratory for Microbiology, Marburg, Germany
    Appl Environ Microbiol 78:5753-62. 2012
    Bacillus subtilis synthesizes large amounts of the compatible solute proline as a cellular defense against high osmolarity to ensure a physiologically appropriate level of hydration of the cytoplasm and turgor...
  93. doi Differential levels of transcription of p53-regulated genes by the arginine/proline polymorphism: p53 with arginine at codon 72 favors apoptosis
    Byeong Seon Jeong
    Cancer Institute of New Jersey, University of Medicine and Dentistry of New Jersey, New Brunswick, New Jersey, USA
    FASEB J 24:1347-53. 2010
    ..the transcriptional pattern of p53-regulated genes, the human isogenic cell lines harboring p53 with either the proline or arginine at codon 72 were employed to activate p53-mediated transcription...
  94. doi Exogenous proline and glycinebetaine increase antioxidant enzyme activities and confer tolerance to cadmium stress in cultured tobacco cells
    Mohammad Muzahidul Islam
    Graduate School of Natural Science and Technology, Okayama University, Okayama 700 8530, Japan
    J Plant Physiol 166:1587-97. 2009
    ..Up-regulation of the antioxidant defense system induced by proline and glycinebetaine (betaine) alleviates the damaging effects of oxidative stress in plants...
  95. doi Exogenous proline and trehalose promote recovery of rice seedlings from salt-stress and differentially modulate antioxidant enzymes and expression of related genes
    Noppawan Nounjan
    Genomics and Proteomics Research Group for Improvement of Salt tolerant Rice, Department of Biology, Khon Kaen University, Khon Kaen 40002, Thailand
    J Plant Physiol 169:596-604. 2012
    b>Proline (Pro) and Trehalose (Tre) function as compatible solutes and are upregulated in plants under abiotic stress...
  96. doi Increasing nitric oxide content in Arabidopsis thaliana by expressing rat neuronal nitric oxide synthase resulted in enhanced stress tolerance
    Hai Tao Shi
    Key Lab of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, PR China
    Plant Cell Physiol 53:344-57. 2012
    ..by changes in physiological parameters including reduced water loss rate, reduced stomatal aperture, and altered proline and malondialdehyde content...
  97. ncbi Salt stress increases the expression of p5cs gene and induces proline accumulation in cactus pear
    Claudia O Silva-Ortega
    División de Biologia Molecular, Instituto Potosino de Investigacion Cientifica y Tecnologica, Camino a la Presa de San José 2055, San Luis Potosi, Mexico
    Plant Physiol Biochem 46:82-92. 2008
    b>Proline (Pro) is one of the most accumulated osmolytes in salinity and water deficit conditions in plants...
  98. ncbi Proline accumulation and transcriptional regulation of proline biosynthesis and degradation in Brassica napus
    Xingning Xue
    Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, China
    BMB Rep 42:28-34. 2009
    To understand the molecular mechanism underlying proline accumulation in Brassica napus, cDNAs encoding Delta(1)-pyrroline-5-carboxylate synthetase (BnP5CS), ornithine delta-aminotransferase (BnOAT) and proline dehydrogenase (BnPDH) were ..
  99. ncbi Isolation and characterisation of proline/arginine-rich cathelicidin peptides from ovine neutrophils
    Rachel C Anderson
    Biotechnology Group, Institute of Technology and Engineering, Massey University, Private Bag 11 222, Palmerston North, New Zealand
    Biochem Biophys Res Commun 312:1139-46. 2003
    ..In this work numerous proline/arginine-rich cathelicidin peptides were purified, including the originally predicted OaBac5 and another OaBac5 ..
  100. doi A single conserved proline residue determines the membrane topology of stomatin
    Ivan Kadurin
    Institute of Physiology II, University of Wurzburg, Röntgenring 9, Wurzburg, Germany
    Biochem J 418:587-94. 2009
    ..It has been proposed that a highly conserved proline residue in the middle of the hydrophobic domain directly binds cholesterol and that cholesterol binding is ..
  101. ncbi Possible involvement of put A gene in Helicobacter pylori colonization in the stomach and motility
    Kazuhiko Nakajima
    Department of Infection Control and Prevention, Hyogo College of Medicine, Hyogo, Japan
    Biomed Res 29:9-18. 2008
    ..pylori in the stomach remains to be clarified. H. pylori shows rather high respiratory activity with L-proline and significantly large amounts of L-proline are present in the gastric juice from H. pylori infected patients...

Research Grants72

  1. Discovery metabolite profiling of the prolyl peptidases
    Alan Saghatelian; Fiscal Year: 2009
    ..Furthermore, the application of DMP to peptidases will demonstrate the generality of this approach for the future characterization of medically relevant enzymes and signaling pathways. ..
  2. Targeting Galectin-3 with novel drugs for treatment of eczema
    Fu Tong Liu; Fiscal Year: 2010
    ..is unique among others in possessing chimeric structure consisting of an N-terminal region that contains proline-rich repeats and a C-terminal carbohydrate-recognition domain...
  3. Impact of the P479L Variant in CPT1A on Infant Mortality in Alaska
    David M Koeller; Fiscal Year: 2011
    ..1436C?T), which results in a proline to leucine substitution at amino acid 479 (p.P479L)...
  4. REGULATION AND FUNCTION OF STE20-RELATED PROTEIN KINASES
    Melanie H Cobb; Fiscal Year: 2012
    ..The Ste20 protein kinase oxidative stress responsive 1 (OSR1) and its homolog the serine, proline, alanine-rich kinase (SPAK) bind to WNK1 in cells, are WNK1 substrates, and are activated by WNK1 phosphorylation...
  5. Induction and maintenance of regulatory T cells
    Mitchell Kronenberg; Fiscal Year: 2013
    ..Cbl-b is composed of an N-terminal tyrosine kinase binding domain, a RING finger, and C-terminal proline-rich sequences...
  6. Alternative Macrophage Activation Limits Immunopathology
    DEBROSKI R HERBERT; Fiscal Year: 2011
    ..AAMF selectively express arginases (isoforms I and II) to metabolize L-arginine into polyamines and proline, implicating AAMF as regulators of cellular proliferation and fibrosis during the wound healing response and ..
  7. Genetic &Functional Analyses of Hypertension Susceptibility Genes
    YEN PEI CHRISTY CHANG; Fiscal Year: 2013
    ..indicated that members of this network, such as with no lysine kinases (WNKs) and STK39-encoded Ste20-related proline-alanine-rich kinase (SPAK), might have unknown isoforms that are renal-segment specific and the distribution and ..
  8. International Symposium on Proline Metabolism and Human Health
    Donald F Becker; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): The main goals of holding a meeting on proline metabolism is to cover a diverse breadth of topics related to human health and disease and to increase the network of researchers in the field...
  9. VEGF Regulation of Hepatic Erythropoietin Synthesis
    Calvin Kuo; Fiscal Year: 2009
    ..signals involving the hormone erythropoietin (Epo), and sensing of hypoxia through inhibition of both HIF-1alpha proline hydroxylation and its subsequent interaction with the Von Hippel-Lindau (VHL) protein...
  10. MIF AND THE HOST RESPONSE TO INFECTION
    RICHARD J BUCALA; Fiscal Year: 2013
    ..to infection of a genetically-engineered "knockin" mouse that produces an MIF protein in which the catalytic proline that mediates tautomerase activity has been replaced by glycine (P1G-MIF)...
  11. Using Mass Spectroscopy to Characterize Transcription Proteins and F2-Isoprostane
    BETTYE HENNINTON; Fiscal Year: 2011
    ..A comparison of the sequences of MLL3 and Gcrlp indicates that MLL3 also has serine-proline-rich regions in similar areas of the amino acid sequence...
  12. Regulation of Lung Fibrosis by Alternatively Activated Macrophages and Arginase-1
    ROBERT MATTHEW TIGHE; Fiscal Year: 2013
    ..Arginase-1 is responsible for the conversion of L-arginine to proline and polyamines. Polyamines are involved in cell proliferation while proline is a direct precursor of collagen...
  13. The consequences of loricrin deficiency on epidermal barrier function
    DENNIS ROOP; Fiscal Year: 2013
    ..We discovered that several known CE components, such as the small proline rich proteins (Sprrs) and repetin were induced to compensate for the loss of loricrin...
  14. Myosin 15:Genetics, Pathology and Therapeutic Potential
    Sally A Camper; Fiscal Year: 2013
    ..isoforms of MYO15 that are generated by alternative splicing, including the presence or absence of a large proline-rich region N-terminal to the motor domain of MYO15...
  15. Immunomodulatory effects of arginine supplementation in colitis and colon cancer
    Keith T Wilson; Fiscal Year: 2013
    ..by either ornithine decarboxylase (ODC) to generate polyamines, or ornithine aminotransferase (OAT) to generate L-proline (L-Pro)...
  16. Mechanisms of Hormonal Regulation of the Sodium Chloride Cotransporter
    Robert S Hoover; Fiscal Year: 2013
    ..With-No-Lysine Kinase 1 and 4 (WNK1 and WNK4), Ste20-related proline alanine-rich kinase (SPAK) and Oxidative Stress Response-1 (OSR1) are also important regulators of NCC, and there ..
  17. Regulation of Osteoblast Function by Megakaryocytes: Key Signaling Proteins
    Melissa A Kacena; Fiscal Year: 2013
    ..Recently, we discovered that MKs regulate the temporal expression of two isoforms of the proline-rich tyrosine kinase 2 (Pyk2), a key protein kinase involved in signaling downstream of activated integrins, and ..
  18. Histone proline isomerization and gene regulation
    Nobuaki Kikyo; Fiscal Year: 2010
    ..In addition, the results will facilitate the progress of nuclear reprogramming and regenerative medicine, especially creation of patients-derived rejection-free tissues for the purpose of transplantation. ..
  19. Pharmacogenetics of ACE inhibitor-associated angioedema
    NANCY JOAN BROWN; Fiscal Year: 2013
    ..a cell-surface protease, preferentially cleaves dipeptides from the amino terminus of peptides with a penultimate proline or alanine, including the incretins, vasoactive peptides such as substance P, and cytokines...
  20. Cannabinoid Mechanisms in Human Gastrointestinal Motor and Sensory Functions
    MICHAEL L CAMILLERI; Fiscal Year: 2010
    ..single nucleotide polymorphism (SNP) in the human FAAH gene (385C to A), in homozygous form, converts a conserved proline residue in FAAH to threonine (P129T), and reduces FAAH protein expression...
  21. The molecular basis for a broad range of phenotypes related to hTAS2R38
    Sarah V Lipchock; Fiscal Year: 2012
    ..Variants of the TAS2R38 gene are best-documented example. Individuals expressing the amino acid combination proline- alanine-valine (PAV) find compounds with a thiourea moiety, such as 6-n-propylthiouracil (PROP), to be extremely ..
  22. Functional Analysis of p53 Polymorphic Variants
    Maureen E Murphy; Fiscal Year: 2013
    ..A common polymorphism exists at codon 72, encoding either arginine (R72) or proline (P72)...
  23. Pin1 prolyl isomerase regulates endothelial nitric oxide synthase
    Richard C Venema; Fiscal Year: 2013
    ..Our preliminary data give rise to the hypothesis that proline-directed phosphorylation of eNOS at S116 promotes binding of the Pin1 prolyl isomerase and Pin1-induced ..
  24. Ac-SDKP for Treatment of Acute Stroke
    Zheng Gang Zhang; Fiscal Year: 2013
    ..Our preliminary data indicate that N-acetyl- seryl-aspartyl-lysyl-proline (Ac-SDKP), a peptide normally presented in human plasma, in combination with tPA reduced infarct volume by more ..
  25. Novel Approaches in Treatment of Vascular Injury Following Balloon Angioplasty
    YEVGENIYA EMRE KOSHMAN; Fiscal Year: 2013
    ..The focal adhesion kinase family of nonreceptor protein tyrosine kinases (PTKs) - focal adhesion kinase (FAK) and proline-rich tyrosine kinase-2 (PYK2) - play a central role in modulating VSMC signal transduction...
  26. Mechanistic and Structure-Function Studies of Human DNA Polymerase Lambda
    Zucai Suo; Fiscal Year: 2011
    ..nuclear localization signal motif, a breast cancer susceptibility protein BRCA1 C- terminal (BRCT) domain, a Proline-rich domain, and a C-terminal polymerase 2-like domain...
  27. Immune-based nutrient deprivation and neurodegenerative disease
    Carol Anne Colton; Fiscal Year: 2013
    ..are featured in immunosuppression;namely arginase (Arg) that uses arginine to make ornithine for polyamine and proline production and Indoleamine dioxygenase (IDO) that uses tryptophan to produce kyneurine...
  28. Resolution of Epithelial Cell Hyperplasia
    Yohannes Tesfaigzi; Fiscal Year: 2013
    ..Deleting or mutating the proline residue within the corresponding region of murine p53 had the same effect in mouse embryo fibroblasts...
  29. Functional characterization of a novel gene for non-alcoholic fatty liver disease
    ALEXIS L GORDEN; Fiscal Year: 2011
    ..This non-synonymous SNP converts serine to proline at position 92 of the neurocan (NCAN) gene...
  30. Epigenetic Changes Link COPD and Lung Cancer
    Yohannes Tesfaigzi; Fiscal Year: 2013
    ..capacity and specific genes within this pathway as determinants for gene methylation in sputum and that the proline variant at codon 72 of p53 is protective from mucous cell hyperplasia and decline of pulmonary function in the ..
  31. Lipid electrophile adduction of Pin1 and effects on substrate binding &activity
    Christopher D Aluise; Fiscal Year: 2013
    ..Furthermore, I propose that Pin1 modification by electrophiles results in an inability to catalyze the proline-directed isomerization of several Raf1 moieties necessary for dephosphorylation by protein phosphatase 2A, ..
  32. A novel proteolytic system of pulmonary inflammation
    Amit Gaggar; Fiscal Year: 2013
    ..We have previously demonstrated that proline-glycine-proline (PGP) peptides can cause a robust PMN influx into the airways of mice;this cellular specificity ..
  33. PGP, A Possible Biomarker for COPD Exacerbations and or Progression
    J Edwin Blalock; Fiscal Year: 2013
    ..MMPs) and prolyl endopeptidase (PE) which degrade collagen and generate the PMN-specific matrikine, proline-glycine-proline (PGP)...
  34. Analysis of Proline Isomerization in Epigenetics
    ROB CARL OSLUND; Fiscal Year: 2013
    ..by applicant): The proposed research in this training plan will focus on studying the effects of cis-trans proline isomerization on chromatin structure and function...
  35. Immunophilins regulate SOC entry channels in pulmonary endothelial cells
    Donna L Cioffi; Fiscal Year: 2013
    ..electrophysiology to measure calcium channel function;nuclear magnetic resonance spectroscopy to measure proline- containing peptide bond isomerization;video microscopy, permeability measurements and resistance measurements to ..
  36. Yeast Ess1, a Conserved PPlase Essential for Mitosis
    Steven D Hanes; Fiscal Year: 2010
    ..PPIases catalyze the cis/trans isomerization of the peptide bond that precedes the cyclic amino acid proline. Prolyl-isomerization results in conformational changes that affect the folding of newly synthesized proteins and ..
  37. Autocoids in Hypertension: Pathogenesis and End Organ Damage
    Oscar A Carretero; Fiscal Year: 2013
    ..In Project I, using Dahl SS rats, we will study whether N-acetyl-seryl-aspartyl-lysyl-proline protects against EOD by decreasing adaptive immunity...
  38. Glycoregulation of Skp1 in the cytoplasm and nucleus
    Christopher M West; Fiscal Year: 2012
    ..six enzymatic steps resulting in the assembly of a pentasaccharide attached to a highly conserved residue of proline. This modification, with a structural richness rivaling that of a peptide, is hypothesized to target only Skp1 ..
  39. Regulation of endocytosis by ubiquitin and ubiquitin-binding proteins
    LINDA A HICKE; Fiscal Year: 2010
    ..discovered to act as UBDs, whereas SH3 domains were previously thought to act almost exclusively by recognizing proline-rich peptides. The known ubiquitin-binding SH3 domains are components of the endocytic machinery...
  40. Role of Arginase in Inflammation and Trauma
    SIDNEY MACHEN MORRIS; Fiscal Year: 2012
    ..are of particular interest because they can affect the synthesis not only of NO but also of polyamines and proline, which are essential for cell proliferation and collagen synthesis, respectively...
  41. Cytosolic Proline Hydroxylation and Glycosylation
    Christopher M West; Fiscal Year: 2013
    ..Aim 3 will employ gene and inhibitor synthetic studies to address the linkage of Skp1 modification to ubiquitination and degradation activities in developmental regulation. ..
  42. FOXP3 Structure, Function and Regulatory Activity in T cells
    Troy Torgerson; Fiscal Year: 2009
    ..and also contains a C2H2 Zinc finger domain, a leucine zipper domain, and an amino-terminal domain rich in proline. Little is known about gene transcription events initiated by FOXP3 in T cells or about the molecular mechanisms ..
  43. Modulation of conjunctival goblet cell differentiation by immunoregulatory cells
    Cintia Sade de Paiva; Fiscal Year: 2010
    ..goblet cell and CD4+ cell density and expression of the goblet cell mucin MUC5AC and metaplasia marker small proline rich residue 2 protein (SPRR-2) in conjunctival sections from normal and dry eye mice...
  44. TOTAL SYNTHESIS AND BIOSYNTHESIS OF BIOACTIVE SUBSTANCES
    Robert Michael Williams; Fiscal Year: 2013
    ..tools of total synthesis to fully elucidate the biosynthetic pathway from the primary amino acids (isoleucine, proline, and tryptophan) and mevalonate-derived isoprene moieties to the polycyclic indole alkaloids comprising the ..
  45. PspA: A Potential Pneumococcal Vaccine Component
    David E Briles; Fiscal Year: 2012
    ..Finally, the proposal includes an expanded investigation of our observation that immunization with the proline-rich (PR) domain of PspA can elicit protection against infection...
  46. Role of WRN Protein in Cigarette Smoke-Induced Cellular Senescence and Emphysema.
    Toru Nyunoya; Fiscal Year: 2011
    ..The unique polypeptide sequences enriched in proline (P), glutamic acid (E), serine (S) and threonine (T) (PEST) residues are often targeted for protein degradation...
  47. Essential Hypertension and Human Skin Blood Flow
    Lacy M Alexander; Fiscal Year: 2013
    ..pathogenesis of hypertension-induced vessel wall remodeling through increased L-ornithine-mediated polyamine and proline synthesis...
  48. Generation of an ITK Biosensor Tool Box
    Constantine D Tsoukas; Fiscal Year: 2010
    ..One is an intramolecular fold resulting from the binding of the SH3 domain to an upstream proline-rich region, whereas the other is a reciprocal dimerization due to the interaction of the SH2 and SH3 domains...
  49. Molecular Assembly on the Cell Surface of Actinomyces
    Hung Ton-That; Fiscal Year: 2013
    ..functions, facilitating the assembly of type 1 fimbriae and directly mediating bacterial adherence to salivary proline-rich proteins known to coat the tooth surface...
  50. Structural Studies of Triple-Helical Proteins
    BARBARA M BRODSKY; Fiscal Year: 2013
    ..an existing recombinant bacterial collagen system as a model for human collagen by introducing appropriate proline hydroxylation and by establishing the capacity to form heterotrimers using a coiled coil domain for chain ..
  51. Combinatorial Post-Translational Modifications of RNA Polymerase II
    ADAM LOTHROP; Fiscal Year: 2013
    ..Much as phosphorylation of the CTD is essential for RNAP II function, proline isomerization by the peptidyl prolyl isomerase Ess1 (Pin1 in humans) is essential for yeast viability...
  52. NMR studies of the effects of mode of birth on vaginal elastin
    Gregory Boutis; Fiscal Year: 2013
    ..The first aim of the proposal is to measure the structural and dynamical properties of lysine, proline, valine, glycine and alanine rich segments of elastin of the vagina following various modes of delivery...
  53. Genes That Regulate Neuronal Cell Death
    Robert Freeman; Fiscal Year: 2009
    ..makes it an important regulator of cell signaling pathways mediated by c-Jun N-terminal kinases (JNK) and other proline-directed protein kinases...
  54. DnaK Protein Inhibitor Antimicrobials
    Michael Sturgess; Fiscal Year: 2003
    ..The best molecules to study these processes are the short, proline-rich antibacterial peptides originally isolated from insects...
  55. Reduced gluten cereal grains
    CHARLES PAUL MOEHS; Fiscal Year: 2013
    ..Both of these conditions are triggered by gluten, a complex mixture of proline and glutamine-rich seed storage proteins found in wheat, barley and rye...
  56. Phosphorylation of Prion Protein as a novel mechanism for conversion
    Andrea C LeBlanc; Fiscal Year: 2010
    ..purified recombinant human, mouse and hamster prion protein can be phosphorylated by the neuronal specific and proline-directed cyclin dependent kinase-5 (cdk5) in vitro...
  57. Mechanism of LTBP4 in muscular dystrophy
    Ermelinda Ceco; Fiscal Year: 2012
    ..We identified a DNA variant in the proline-rich region of murine LTBP4 that segregates with mild and severe forms of muscular dystrophy in mice...
  58. Analytical tools for the analysis of clustered O-glycans in clinical samples
    Matthew B Renfrow; Fiscal Year: 2013
    ..within this class of glycoproteins is analytically challenging due to the clustered serine, threonine, and often proline residues...
  59. The role of salivary proteins in taste and feeding
    Ann Marie Torregrossa; Fiscal Year: 2013
    ..Seventy percent of all salivary proteins make up a class of proteins referred to as proline-rich proteins (PRPs)...
  60. In vivo gene silencing in salivary glands
    Sven Ulrik Gorr; Fiscal Year: 2009
    ..Thus, this application is based on the findings that acidic sulfated proteoglycans and basic proline-rich proteins can regulate protein storage, apparently by a pH- dependent mechanism...
  61. The role of GABA-mimetic metabolites in neurodevelopmental and neuropsychiatric d
    Joseph A Gogos; Fiscal Year: 2013
    ..One cause of hyperprolinemia is loss-of-function mutations of the PRODH gene, which is involved in L-proline degradation. The PRODH gene maps in the 22q11.2 locus, and is heterozygously deleted in the 22q11...
  62. The Importance of Prolyl 3-hydroxylation in recessive Osteogenesis Imperfecta
    ERICA PAIGE HOMAN; Fiscal Year: 2013
    ..CRTAP and P3H1 form a complex with cyclophilin B (CypB) and this complex functions to 3-hydroxylate a single proline residue on the alpha 1 chain of type I collagen[2]...
  63. Functional and structural characterization of spliceosomal cyclophilins
    Tara L Davis; Fiscal Year: 2011
    ..understanding of splicing mechanism to include the roles of cyclophilin specific protein:protein interactions and proline isomerization within the spliceosome...
  64. Peptide Derivatives to treat Urinary Tract Infections
    Laszlo Otvos; Fiscal Year: 2003
    ..In this desperate situation the insect-derived cationic proline-rich antibacterial peptide family can come to the rescue...
  65. THE ROLE OF PIN1 IN MITOSIS WITH DESIGNED INHIBITORS
    FELICIA ETZKORN; Fiscal Year: 2002
    ..Pin 1, a newly discovered regulator of mitosis signal transduction, is an enzyme that acts on phosphoserine-proline amides...
  66. Aminoacyl-tRNA synthetases in Archaea
    Ya Ming Hou; Fiscal Year: 2006
    ..prolyl-tRNA synthetase (ProRS) of the archaeon Methanococcus jannaschii, which has the ability to activate both proline and cysteine and catalyze aminoacylation of tRNA with proline and cysteine...
  67. FUNCTIONAL ANALYSIS OF AN RNA STRUCTURAL MOTIF
    Ya Ming Hou; Fiscal Year: 2005
    ..embedded within the sequence framework of a prolyltRNA synthetase (ProRS) that has the ability to activate both proline and cysteine and catalyze aminoacylation of tRNA with proline and with cysteine...
  68. GENE EXPRESSION OF SALIVARY GLAND SPECIFIC GENES
    David Ann; Fiscal Year: 1993
    ..of the research outlined by this proposal are (1) to understand why the expression of a given set of gene (proline-rich protein genes) is limited to the salivary glands; and (2) to determine how this expression is modulated in ..
  69. MOLECULAR AND CELLULAR MECHANISMS OF CRANIOSYNOSTOSIS
    MICHAEL IGNELZI; Fiscal Year: 2000
    ..mutation in the homeodomain of MSX2 is associated with craniosynostosis, Boston type; affected individuals bear a proline to histidine substitution in position 7 (Pro7His) of the MSX2 homeobox...
  70. Proline uptake in staphylococcus aureus pathogenesis
    WILLIAM SCHWAN; Fiscal Year: 2002
    ..aureus is able to infect so many different tissues within the human body, this grant proposes to study proline transport in S. aureus as a means to study the role proline transporters play in the pathogenesis of the bacteria...
  71. MULTIPLE CHIMERIC TRANSCRIPTS IN THE T(3--21)
    Giuseppina Nucifora; Fiscal Year: 1999
    ..ETO also is a transcription factor based on proline-rich regions and two zinc finger motifs...
  72. PROFILIN I--AN ESSENTIAL PROTEIN IN CELLS AND ANIMALS
    Pascal Goldschmidt Clermont; Fiscal Year: 1999
    ..in vitro: (1) profilin binds to monomeric actin (stoichiometry; one to one): (ii) profilin binds to poly-L-proline (PLP,one profilin binds to a stretch of greater than prolines); (iii) profilin binds membranes containing ..