Genomes and Genes
Summary: A standard reagent for the determination of reactive sulfhydryl groups by absorbance measurements. It is used primarily for the determination of sulfhydryl and disulfide groups in proteins. The color produced is due to the formation of a thio anion, 3-carboxyl-4-nitrothiophenolate.
Publications176 found, 100 shown here
- Inactivation of hantaviruses by N-ethylmaleimide preserves virion integrityTomas Strandin
Peptide and Protein Laboratory, Infection Biology Research Program, Haartman Institute, University of Helsinki, Helsinki, Finland
J Gen Virol 92:1189-98. 2011..In contrast to hantaviruses, NEM did not abolish Uukuniemi phlebovirus infectivity to the same extent. This indicates differences in the use of free thiols in virus entry among members of the family Bunyaviridae...
- The catalytic activity of protein disulfide isomerase is involved in human immunodeficiency virus envelope-mediated membrane fusion after CD4 cell bindingE Fenouillet
Centre National de la Recherche Scientifique, Faculte de Medecine Nord, Marseille, France
J Infect Dis 183:744-52. 2001..The fact that the PDI catalytic activity present on lymphocytes is required for fusion supports the hypothesis that catalysts assist post-CD4 cell binding conformational changes within Env...
- Thiol/disulfide exchange is required for membrane fusion directed by the Newcastle disease virus fusion proteinSurbhi Jain
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
J Virol 81:2328-39. 2007..In the presence of HN protein, only the reduced form may proceed to refold into additional intermediates, leading to the fusion of membranes...
- Inhibition of human immunodeficiency virus infection by agents that interfere with thiol-disulfide interchange upon virus-receptor interactionH J Ryser
Department of Pathology, Boston University School of Medicine, MA 02118
Proc Natl Acad Sci U S A 91:4559-63. 1994..These findings suggest additional approaches to impede cell infection by HIV...
- Molecular docking studies of dithionitrobenzoic acid and its related compounds to protein disulfide isomerase: computational screening of inhibitors to HIV-1 entryUthaman Gowthaman
Bioinformatics Centres of BTISnet at Pondicherry University and IIT Delhi, India
BMC Bioinformatics 9:S14. 2008..This study attempts to perceive the mode of binding of dithionitrobenzoic acid (DTNB), and its structurally related compounds on PDI enzyme.
- Antiviral propierties of 5,5'-dithiobis-2-nitrobenzoic acid and bacitracin against T-tropic human immunodeficiency virus type 1Humberto H Lara
Laboratorio de Inmunología y Virología, Departamento de Microbiologia e Inmunologia, Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico
Virol J 8:137. 2011..These drugs proved to be leading compounds for further studies against HIV showing antiviral characteristics of interest...
- Reversible reaction of 5,5'-dithiobis(2-nitrobenzoate) with the CysF9beta sulfhydryl groups of the hemoglobins of the domestic cat: variation of the equilibrium and reverse rate constants with pHKehinde Onwochei Okonjo
Department of Chemistry, University of Ibadan, Ibadan, Nigeria
Biophys Chem 121:65-73. 2006..2+/-0.2 and 9.4+/-0.1 in the major hemoglobin and 6.7+/-0.3 and 8.4+/-0.1 in the minor hemoglobin...
- On the nature of conformational openings: native and unfolded-state hydrogen and thiol-disulfide exchange studies of ferric aquomyoglobinZ Feng
Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 E. Adams Street, Syracuse, NY 13210, USA
J Mol Biol 314:153-66. 2001..The C73 thiol group is exposed by a low-energy fluctuation that does not expose its amide group to exchange...
- Active site of dihydroorotate dehydrogenase A from Lactococcus lactis investigated by chemical modification and mutagenesisO Bjornberg
Center for Enzyme Research, Institute of Molecular Biology, and Centre for Crystallograpic Studies, Department of Chemistry, University of Copenhagen, Copenhagen, Denmark
Biochemistry 36:16197-205. 1997..The possible acid-base chemistry of Cys 130 is compared to previous work on mammalian dihydropyrimidine dehydrogenases, flavoenzymes, which catalyze the reversed reaction, namely the reduction of pyrimidine bases...
- Regulation of PTP1B via glutathionylation of the active site cysteine 215W C Barrett
Laboratory of Biochemistry, Section of Metabolic Regulation, National Heart, Lung and Blood Institute, Bethesda, Maryland 20892, USA
Biochemistry 38:6699-705. 1999..Glutathionylation of the cysteine sulfenic derivative will prevent the enzyme from further oxidation to its irreversible forms, and constitutes an efficient regulatory mechanism...
- Mass spectrometric determination of the cleavage sites in Escherichia coli dihydroorotase induced by a cysteine-specific reagentR Daniel
Laboratoire de Bioorganique et Biotechnologies associé au Centre National de la Recherche Scientifique, Ecole Nationale Superieure de Chimie de Paris, 11 rue P and M Curie, 75231 Paris Cedex 05, France
J Biol Chem 272:26934-9. 1997..Consistent with literature data, we assume that cysteine 221 and cysteine 265, along with the neighboring cysteines 263 and 268 arranged in cluster, are potential ligands of structural zinc of E. coli dihydroorotase...
- Roles for cysteine residues in the regulatory CXXC motif of human mitochondrial branched chain aminotransferase enzymeMyra E Conway
Department of Biochemistry, Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, North Carolina 27157, USA
Biochemistry 43:7356-64. 2004..Thus, C315 appears to be the sensor for redox regulation of BCAT activity, whereas C318 acts as the "resolving cysteine", allowing for reversible formation of a disulfide bond...
- Determination of glutathione, cysteine and N-acetylcysteine in rabbit eye tissues using high-performance liquid chromatography and post-column derivatization with 5,5'-dithiobis(2-nitrobenzoic acid)M J Nozal
Department of Analytical Chemistry, Faculty of Sciences, University of Valladolid, Spain
J Chromatogr A 778:347-53. 1997..The detection limits are 0.21, 0.92 and 0.61 mumol/g wet sample for glutathione, cysteine and N-acetylcysteine, respectively...
- Purification, tandem mass characterization, and inhibition studies of oxidosqualene-lanosterol cyclase enzyme from bovine liverTung Kung Wu
Department of Biological Science and Technology, National Chiao Tung University, 300 Hsin Chu, Taiwan, China
Arch Biochem Biophys 421:42-53. 2004..The stoichiometry of histidine modification and the extent of inactivation showed that two essential histidine residues per active site are necessary for complete bovine liver OSC activity...
- Synthesis and characterization of a novel reagent containing dansyl group, which specifically alkylates sulfhydryl group: an example of application for protein chemistryGo Hasegawa
Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama 226 8501, Japan
J Biochem Biophys Methods 63:33-42. 2005..These data showed that IADC is a useful reagent to specifically identify the positions of a Cys residue(s) in proteins...
- Calcium-dependent cysteine reactivities in the neuronal calcium sensor guanylate cyclase-activating protein 1J Y Hwang
, , Germany
FEBS Lett 508:355-9. 2001..9 microM for Ca(2+) binding to this site with a fast off-rate (t approximately 2 ms). We conclude that the rapid dissociation of Ca(2+) from EF-hand 3 in GCAP-1 triggers activation of guanylate cyclase in rod cells...
- Kinetics of inactivation of glutamate decarboxylase by cysteine-specific reagentsS J McCormick
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Evansville, IN 47712, USA
Acta Biochim Pol 48:573-8. 2001..The characteristics of the inhibition by the three thiol-group reagents supports the idea that cysteinyl residues at the binding sites for the cofactor and/or the substrate are important for enzyme activity in E. coli...
- Disulfiram irreversibly aggregates betaine aldehyde dehydrogenase--a potential target for antimicrobial agents against Pseudomonas aeruginosaRoberto Velasco-García
Laboratorio de Osmorregulación, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autonoma de Mexico, Avenida de Los Barrios, Tlalnepantla, Estado de Mexico, 54090 Mexico, Mexico
Biochem Biophys Res Commun 341:408-15. 2006..methyl-methanethiosulfonate inactivated the enzymes without affecting their stability while the bulkier dithionitrobenzoic acid (DTNB) and bis[diethylthiocarbamyl] disulfide (disulfiram) induced enzyme dissociation--at 23 degrees C--..
- Implications of the role of reactive cystein in arginine kinase: reactivation kinetics of 5,5'-dithiobis-(2-nitrobenzoic acid)-modified arginine kinase reactivated by dithiothreitolJi Cheng Pan
Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, PR China
Biochem Biophys Res Commun 317:539-44. 2004..The reactive cysteine of arginine kinase may play an important role not in the binding to the transition-state analog but in the conformational changes caused by the transition-state analog...
- Identification of essential residues in 2',3'-cyclic nucleotide 3'-phosphodiesterase. Chemical modification and site-directed mutagenesis to investigate the role of cysteine and histidine residues in enzymatic activityJ Lee
Department of Biochemistry, McGill University, Montreal, Quebec H3G 1Y6, Canada
J Biol Chem 276:14804-13. 2001..Taken together, these results demonstrate that both histidines assume critical roles for catalysis...
- Modifications of cysteine residues in the solution and membrane-associated conformations of phosphatidylinositol transfer protein have differential effects on lipid transfer activityJ M Tremblay
Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, Kansas 66160 7421, USA
Biochemistry 40:9151-8. 2001..Furthermore, the inhibition of PITP activity by thiol-modifying reagents is a result of steric hindrance of phospholipid substrate binding...
- Different metabolizing ability of thiol reactants in human and rat blood: biochemical and pharmacological implicationsR Rossi
Department of Neuroscience, Pharmacology Unit, Via A. Moro 4, University of Siena, Italy
J Biol Chem 276:7004-10. 2001..This is thought to influence significantly the biochemical, pharmacological, and toxicological effects of some drugs...
- Structural and functional studies of vertebrate metallothioneins: cross-talk between domains in the absence of physical contactClemente Capasso
CNR, Institute of Protein Biochemistry, via P Castellino 111 I 80131 Naples, Italy
Biochem J 391:95-103. 2005..Taken together, these results provide a strong argument in favour of the interaction of the two domains in the MT molecule, in spite of the elusive evidence provided by the structural analyses...
- Changes in sulfhydryl content of egg white proteins due to heat and pressure treatmentIesel Van der Plancken
Laboratory of Food Technology, Department of Food and Microbial Technology, Faculty of Applied Bioscience and Engineering, Katholieke Universiteit Leuven, Kasteelpark Arenberg 22, B 3001 Heverlee, Belgium
J Agric Food Chem 53:5726-33. 2005..Treatment conditions resulting in extensive protein insolubilization were accompanied by a transfer of free sulfhydryl groups from the soluble to the insoluble protein fraction. These SH groups were mainly accessible to DTNB...
- The role of cysteine residues in tellurite resistance mediated by the TehAB determinantM Dyllick-Brenzinger
Structural Biology Research Group, University of Calgary, Calgary, Alberta, T2N 1N4, Canada
Biochem Biophys Res Commun 277:394-400. 2000..Studies using native and SDS denaturing PAGE under reducing and oxidizing conditions suggested that a cysteine in TehB is involved in binding tellurite...
- The kinetic study of arginine kinase from the sea cucumber Stichopus japonicus with 5,5'-dithiobis-(2-nitrobenzoic acid)Zhao Feng
Department of Biological Science and Biotechnology, School of Life Science and Engineering, Tsinghua University, Beijing 100084, China
Int J Biol Macromol 36:184-90. 2005..The reactive cysteine of AK, which was proposed to be Cys274, may play an important role not in the binding of the transition-state analog but in the conformational changes caused by the transition-state analog...
- Cleavage of disulfide-linked fetuin-bisphosphonate conjugates with three physiological thiolsSufeng Zhang
Department of Chemical and Materials Engineering, Faculty of Engineering, University of Alberta, Edmonton, AB T6G 2G6, Canada
Biomacromolecules 6:2800-8. 2005..In conclusion, disulfide-linked fetuin-thiolBP conjugates exhibited a high affinity to HA, which was readily lost upon cleavage with thiols found in physiological milieu...
- Reversible reaction of 5,5'-dithiobis(2-nitrobenzoate) with the hemoglobins of the domestic cat: acetylation of NH3+ terminal group of the beta chain transforms the complex pH dependence of the forward apparent second order rate constant to a simple formKehinde O Okonjo
Department of Chemistry, University of Ibadan, Ibadan, Nigeria
Biophys Chem 119:196-204. 2006..Analyses of the simple profiles gave pKas of 6.33 +/- 0.17 and 8.54 +/- 0.5 for HisH21beta and other histidines within a distance of 2 nm of the sulfhydryl, and CysF9beta of the minor hemoglobin, respectively...
- Zinc transfer potentials of the alpha - and beta-clusters of metallothionein are affected by domain interactions in the whole moleculeL J Jiang
Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Seeley G Mudd Building, 250 Longwood Avenue, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 97:2503-8. 2000..The two-domain structure of the whole molecule is important for its interaction with ligands and for control of its reactivity and overall conformation...
- Development and application of serum cholinesterase activity measurement using benzoylthiocholine iodideSusumu Osawa
School of Medicine, Biological Sciences and Technology, Department of Health Sciences, Kyushu University, Higashi, Fukuoka City 812 8582, Japan
Clin Chim Acta 351:65-72. 2005..Many procedures using various artificial substrates have been developed but suffer from problems with substrate specificity and interference by endogenous substances...
- Perturbation of microtubule polymerization by quercetin through tubulin binding: a novel mechanism of its antiproliferative activityKamlesh Gupta
BJM School of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, Powai, Mumbai 400 076, India
Biochemistry 41:13029-38. 2002..The data suggest that quercetin inhibits cancer cells proliferation at least in part by perturbing microtubule functions through tubulin binding...
- A thiol labelling competition experiment as a probe for sidechain packing in the kinetic folding intermediate of N-PGKMatthew J Cliff
Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK
J Mol Biol 364:810-23. 2006..The success of the competition technique in characterizing this kinetic intermediate invites application to other model systems...
- Exploration of the polar microenvironment around the reactive cysteine in rabbit muscle creatine kinaseHua Wei He
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, PR China
Int J Biol Macromol 41:361-8. 2007..Furthermore, the findings in this study may provide a novel explanation for the low pKa value of Cys283...
- Critical flocculation concentrations, binding isotherms, and ligand exchange properties of peptide-modified gold nanoparticles studied by UV-visible, fluorescence, and time-correlated single photon counting spectroscopiesHuan Xie
Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
Anal Chem 75:5797-805. 2003..Full characterization of the composition and stability of peptide-modified metal nanoparticles is an important step in their use as intracellular delivery vectors and imaging agents...
- Antiviral activity of RhoA-derived peptides against respiratory syncytial virus is dependent on formation of peptide dimersPhilip J Budge
Department of Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
Antimicrob Agents Chemother 47:3470-7. 2003....
- Protein disulfide isomerase, a component of the estrogen receptor complex, is associated with Chlamydia trachomatis serovar E attached to human endometrial epithelial cellsC H Davis
Department of Microbiology and Immunology, School of Medicine, University of North Carolina Chapel Hill, Chapel Hill, North Carolina, USA
Infect Immun 70:3413-8. 2002..trachomatis serovar E entry...
- Actin S-glutathionylation: evidence against a thiol-disulphide exchange mechanismIsabella Dalle-Donne
Department of Biology, University of Milan, Milan, Italy
Free Radic Biol Med 35:1185-93. 2003....
- Role of cysteine 306 in the catalytic mechanism of Ascaris suum phosphoenolpyruvate carboxykinaseSandra E Rios
Department of Chemistry and Biochemistry, University of Notre Dame, IN 46556, USA
Arch Biochem Biophys 404:25-37. 2002..Alternatively, mutation of this residue could cause protein changes that interfere with the proper conformation of the nucleotides for optimal catalysis to take place...
- The roles of thiols in the bacterial organomercurial lyase (MerB)Keith E Pitts
Department of Microbiology and Center for Metalloenzyme Studies, The University of Georgia, Athens, Georgia 30602, USA
Biochemistry 41:10287-96. 2002..On the basis of the foregoing we refine the previously proposed S(E)2 mechanism for protonolysis by MerB...
- Single-molecule covalent chemistry with spatially separated reactantsTudor Luchian
Department of Medical Biochemistry and Genetics, The Texas A and M University System Health Science Center, 440 Reynolds Medical Building, TAMU 1114, College Station, TX 77843 1114, USA
Angew Chem Int Ed Engl 42:3766-71. 2003
- Kinetic studies of rat liver hexokinase D ('glucokinase') in non-co-operative conditions show an ordered mechanism with MgADP as the last product to be releasedOctavio Monasterio
Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
Biochem J 371:29-38. 2003..In addition, the straight-line dependence observed when the reciprocal value of the inactivation constant was plotted against the sugar-ligand concentration supports the view that there is just one sugar-binding site in hexokinase D...
- Studies on regulatory functions of malic enzymes. IV. Effects of sulfhydryl group modification on the catalytic function of NAD-linked malic enzyme from Escherichia coliM Yamaguchi
J Biochem 86:325-33. 1979..Based on the above results, the mechanism of the alteration of enzyme activities by sulfhydryl group modification is discussed...
- Studies on regulatory functions of malic enzymes. VII. Structural and functional characteristics of sulfhydryl groups in NADP-linked malic enzyme from Escherichia coli WM Iwakura
J Biochem 86:1239-49. 1979..The protective effect of NADP+ was increased by the addition of Mg2+. Based on the above results, the effects of Mg2+ on the SH-group modification are discussed from the viewpoint of conformational alteration of the enzyme...
- Enzymatically catalyzed disulfide exchange is required for platelet adhesion to collagen via integrin alpha2beta1Judith Lahav
The Coagulation Laboratory, Rabin Medical Center Beilinson Campus, Zabotinski St, Petah Tiqva 49100, Israel
Blood 102:2085-92. 2003..These data suggest that the enzymatically catalyzed adhesion-associated reorganization of disulfide bonds is common to members of the integrin family and specific to this family...
- Nature and environment of the sulfhydryls of membrane-associated D-lactate dehydrogenase of Escherichia coliS R Dowd
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA
Biochim Biophys Acta 1252:278-83. 1995..Cysteine 492 has been identified as containing the sulfhydryl that is accessible to the reagent under both conditions...
- Sulfhydryl groups of glucosamine-6-phosphate isomerase deaminase from Escherichia coliM M Altamirano
Departamento de Bioquimica, Facultad de Medicina, Universidad Nacional Autonoma de Mexico, Mexico City, D F
Arch Biochem Biophys 258:95-100. 1987..The results obtained show that sulfhydryls are not essential for catalysis or allosteric behavior of glucosamine-6-phosphate deaminase...
- Contribution of redox status to hepatitis C virus E2 envelope protein function and antigenicityEmmanuel Fenouillet
UMR 6184, CNRS, Université Aix Marseille II, F 13015, France
J Biol Chem 283:26340-8. 2008..Although E2 function is surprisingly independent of its redox status, its disulfide bonds mask antigenic domains. E2 redox manipulation may contribute to improved vaccine design...
- Characterization of chitosan thiolation and application to thiol quantification onto nanoparticle surfaceI Bravo-Osuna
Laboratoire de Physicochimie, Pharmacotechnie et Biopharmacie, UMR CNRS 8612, Universite Paris Sud 11, Faculte de Pharmacie, 5, rue J B Clement, 92296 Chatenay Malabry, France
Int J Pharm 340:173-81. 2007..It was successfully applied to the quantification of active thiol groups on the surface of poly(isobutyl cyanoacrylates) nanoparticles coated with thiol chitosan...
- Cysteine reactivity and thiol-disulfide interchange pathways in AhpF and AhpC of the bacterial alkyl hydroperoxide reductase systemThomas J Jönsson
Department of Biochemistry, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
Biochemistry 46:5709-21. 2007..In addition, two new constructs have been generated, an AhpF-AhpC complex and a "twisted" form of AhpF, in which redox centers are locked together by stable disulfide bonds which mimic catalytic intermediates...
- Simple shifts in redox/thiol balance that perturb blood coagulationHenry K Bayele
Department of Haematology, Royal Free and University College Medical School, Royal Free Campus, London NW3 2PF, UK
FEBS Lett 510:67-70. 2002..These data also suggest that blood clotting may be influenced by unidentified redox or thiol equilibria...
- Identification and characterization of a critical region in the glycogen synthase from Escherichia coliAlejandra Yep
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, USA
J Biol Chem 279:8359-67. 2004..The results of this work indicate that this region is critical for catalysis and substrate binding...
- Reduced redox state allows prolonged survival of axotomized neonatal retinal ganglion cellsL K Geiger
Department of Ophthalmology and Visual Sciences, University of Wisconsin Medical School, 600 Highland Avenue, Madison, WI 53792, USA
Neuroscience 109:635-42. 2002..Understanding the mechanisms by which ROS signal neuronal death could result in strategies for increasing their long-term survival after axonal injury...
- Role of cysteine residues in 4-oxalomesaconate hydratase from Pseudomonas ochraceae NGJ1Suhong Li
Department of Biomolecular Science, Faculty of Engineering, Gifu University, Gifu, Japan
Biosci Biotechnol Biochem 71:449-57. 2007....
- Purification and characterization of N-acetylneuraminic acid-9-phosphate synthase from rat liverHao Chen
Institute of Molecular Biology and Biochemistry, Free University of Berlin, Arnimallee 22, 14195 Berlin Dahlem, Germany
Glycobiology 12:65-71. 2002....
- Chemical modification studies on arginine kinase: essential cysteine and arginine residues at the active siteWen Jing Zhu
College of Life Science, Shandong Agricultural University, Shandong Taian 271018, People s Republic of China
Int J Biol Macromol 41:564-71. 2007..Using the specific chemical modifier phenylglyoxal (PG) demonstrated that only one Arg, located near the ATP binding site, is essential for the activity of AK...
- Recombinant lysine:N(6)-hydroxylase: effect of cysteine-->alanine replacements on structural integrity and catalytic competenceScott Dick
Department of Chemistry, University of Waterloo, ON, Canada
Biochim Biophys Acta 1594:219-33. 2002..A possible role of rIucD's Cys51 in the modulation of its mono-oxygenase function is discussed...
- Surface accessibility of the 70-kilodalton Chlamydia trachomatis heat shock protein following reduction of outer membrane protein disulfide bondsJane E Raulston
Department of Microbiology, James H Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee 37614 0579, USA
Infect Immun 70:535-43. 2002..The accessibility of the Hsp70 substrate-binding domain may support the hypothesis that this protein plays a role in events following the initial stage of attachment instead of serving as a primary, surface-displayed adhesin...
- DNA damage, apoptosis and cell cycle changes induced by fluoride in rat oral mucosal cells and hepatocytesLing Fei He
Department of Dental Medicine, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei Province, China
World J Gastroenterol 12:1144-8. 2006..To study the effect of fluoride on oxidative stress, DNA damage and apoptosis as well as cell cycle of rat oral mucosal cells and hepatocytes...
- Comparative inhibition by hard and soft metal ions of steroid-binding capacity of renal mineralocorticoid receptor cross-linked to the 90-kDa heat-shock protein heterocomplexM D Galigniana
Departamento de Qu approximately ímica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, and PHROM CONICET, 1428 Buenos Aires, Argentina
Biochem J 341:585-92. 1999..role played by these residues in steroid binding was confirmed by chemical modification of cysteines with dithionitrobenzoic acid, histidines with diethyl pyrocarbonate and acidic amino acids with Woodward's reagent (N-ethyl-5-..
- Glutathione content of in vivo and in vitro matured canine oocytes collected from different reproductive stagesMin Kyu Kim
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea
J Vet Med Sci 69:627-32. 2007..GSH concentrations were determined by the dithionitrobenzoic acid-glutathione disulfide (DTNB-GSSG) reductase recycling assay...
- Irreversible activation of cyclic nucleotide-gated ion channels by sulfhydryl-reactive derivatives of cyclic GMPR L Brown
Neurological Sciences Institute, Oregon Health Sciences University, 1120 NW 20th Avenue, Portland, Oregon 97209, USA
Biochemistry 39:432-41. 2000..Pretreatment of the patch with the sulfhydryl-blocking reagents N-ethylmaleimide (NEM) and bis-dithionitrobenzoic acid (DTNB) prevented covalent activation; the effect of DTNB was reversed by reduction with DTT...
- Effect of oxidative stress on the structure and function of human serum albuminM Anraku
Faculty of Pharmaceutical Sciences, Kumamoto University, Japan
Pharm Res 18:632-9. 2001..In contrast, both the ligand binding property of site II and the esterase-like activity of oxidized HSAs are decreased, most probably due to conformational changes in subdomain IIIA...
- Purification and characterization of acetylcholinesterase from cotton aphid (Aphis gossypii Glover)Fei Li
Key Laboratory of Monitoring and Management of Plant Diseases and Pests, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China
Arch Insect Biochem Physiol 51:37-45. 2002..43 and 11.73 times, respectively. We infer that one or more factors in the crude extract from the resistance strain have more influence on AChE sensitivity. Further study is needed to investigate the basis of these observations...
- Interaction between catalytic and regulatory sites of the pyruvate dehydrogenase from Escherichia coli studied by the ESR techniqueK Graupe
Physiologisch Chemisches Institut, Universitat Tubingen, F R G
Biochim Biophys Acta 999:176-82. 1989..The implications of these results for the allosteric regulation of the pyruvate dehydrogenase complex are discussed...
- Identification of an essential cysteine residue in pyridoxal phosphatase from human erythrocytesG Gao
Department of Biochemistry, University of Louisville School of Medicine, Kentucky 40292
J Biol Chem 269:8234-9. 1994..Residues 5-12 and 15 of this peptide are identical with a sequence of a yeast alkaline p-nitrophenylphosphatase, and the peptide has little homology with other mammalian phosphatases...
- Characterization of a fatty acyl-CoA reductase from Marinobacter aquaeolei VT8: a bacterial enzyme catalyzing the reduction of fatty acyl-CoA to fatty alcoholRobert M Willis
Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322, United States
Biochemistry 50:10550-8. 2011..The substrate cis-11-hexadecenal was reduced in a cooperative manner with an apparent K(m) of ~50 μM, V(max) of ~8 μmol NADP(+) min(-1) (mg protein)(-1), and n ~ 2...
- New findings about Ellman's method to determine cholinesterase activityAlena Komersová
Department of Physical Chemistry, Faculty of Chemical Technology, University of Pardubice, Nám Legií 565, 532 10 Pardubice, Czech Republic
Z Naturforsch C 62:150-4. 2007..Our experiments indicate that this influence of DTNB concentration can be explained by the inhibition of ATCH hydrolysis by DTNB...
- Effect of cadmium, mercury and copper on partially purified hepatic flavokinase of ratD Bandyopadhyay
Indian Institute of Chemical Biology, Calcutta, India
Mol Cell Biochem 167:73-80. 1997....
- Catalytic zinc site and mechanism of the metalloenzyme PR-AMP cyclohydrolaseRobert L D'Ordine
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA
Biochemistry 51:5791-803. 2012..The results are consistent with a Zn(2+)-mediated activation of H(2)O mechanism involving histidine as a general base that has features similar to but distinct from those of previously characterized purine and pyrimidine deaminases...
- Evaluation of protein disulfide conversion in vitro using a continuous flow dialysis systemXinzhao Grace Jiang
Drug Substance Development, Amgen, Thousand Oaks, CA 91320, USA
Anal Biochem 432:142-54. 2013..These are important considerations when evaluating the criticality of product quality attributes...
- Thiol-reactive compounds prevent nonspecific antibody binding in immunohistochemistryArlin B Rogers
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Lab Invest 86:526-33. 2006..These findings provide a molecular basis to improve the specificity of IHC and other immunoassays, and hold implications for antibody-based clinical diagnostics and therapeutics...
- Thiol-disulphide interchange in tubulin: kinetics and the effect on polymerizationP J Britto
Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Biochem J 389:549-58. 2005..Under such conditions, the substituted tubulin forms protofilament-based structures such as microtubules, open tubules, sheets and/or bundles...
- Purification of acetylcholinesterase by 9-amino-1,2,3,4-tetrahydroacridine from human erythrocytesHabibe Budak Kaya
Department of Chemistry, Faculty of Sciences, Ataturk University, 25240 Erzurum, Turkey
Appl Biochem Biotechnol 170:198-209. 2013..25 mM, and the V(max) was 0.090 μmol/mL/min. Maximum binding was achieved at 2 °C with pH 7.4 and an ionic strength of approximately 0.1 M. The capacity for the optimum condition was 0.07 mg protein/g gel for acetylcholinesterase...
- Nitric oxide directly activates GABA(A) receptor function through a cGMP/protein kinase-independent pathway in frog pituitary melanotrophsH Castel
European Institute for Peptide Research (IFRMP 23, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U413, UA CNRS, University of Rouen, Mont-Saint-Aignan, France
J Neuroendocrinol 13:695-705. 2001..The effect of NO can be accounted for by S-nitrosylation/oxidation of thiol groups either directly on the GABA(A) receptor subunits or on a regulatory protein tightly associated with the GABA(A) receptor...
- A colorimetric microplate assay method for high throughput analysis of lipase activitySuk Jung Choi
Department of Chemistry, Kangnung National University, Gangneung 210 702, Korea
J Biochem Mol Biol 36:417-20. 2003..01-0.1 U or 1-10 U. When assaying crude tissue extracts, the reaction of DTNB with non-specific reducing agents created a major source of error. However, this error was corrected by the use of blank samples that did not contain DMPTB...
- A simple assay of taurine concentrations in food and biological samples using taurine dioxygenaseMotoki Matsuda
Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
Anal Biochem 427:121-3. 2012..Taurine dioxygenase can be used for taurine determination in food control, biological research, and diagnoses based on urinary taurine concentration...
- HIV-1 viral load determination based on reverse transcriptase activity recovered from human plasmaAnders Malmsten
Department of Genetics and Pathology, Uppsala University, and Cavidi Tech AB, Uppsala Science Park, SE 751 83 Uppsala, Sweden
J Med Virol 71:347-59. 2003..Being insensitive to genetic drift occurring in HIV, the assay should be of particular use in resource-limited settings, where different subtypes and recombinant HIV strains occur...
- Soybean (Glycine max) urease: significance of sulfhydryl groups in urea catalysisSandeep Kumar
School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi 221005, India
Plant Physiol Biochem 48:746-50. 2010..Finally, when NEM inactivated urease was incubated with sodium fluoride, a time-dependent regain of activity was observed with higher concentrations of fluoride ion...
- Purification and characterization of camel (Camelus dromedarius) milk amylaseEsmail M El-Fakharany
Antibody Laboratory, Protein Research Department, Genetic Engineering and Biotechnology Research Institute, New Borg EL Arab, Alexandria, Egypt
Prep Biochem Biotechnol 39:105-23. 2009..It worked on starch to yield a maltose. Scanning electron microscope images demonstrated a nano-degrading ability on starch granules from various sources (potato, corn, cassava, and rice)...
- [Determination of L-cysteine in enzymatic reaction mixture with pre-column derivatization by high performance liquid chromatography]Zhong Liu
College of Life Science, Nankai University, Tianjin 300071, China
Se Pu 22:231-3. 2004..The linear range was 5-950 micromol/L. The recoveries were 99.7%-100.5% and the relative standard deviations (RSDs) were less than 1.3%. The detection limit was 0.8 micromol/L. The method is simple and accurate...
- Ascorbic acid reduction of microtubule protein disulfides and its relevance to protein S-nitrosylation assaysLisa M Landino
Department of Chemistry, The College of William and Mary, P O Box 8795, Williamsburg, VA 23187 8795, USA
Biochem Biophys Res Commun 340:347-52. 2006..Not only do we describe a new reactivity of ascorbic acid with microtubule proteins but we expose an important limitation when using the biotin switch assay to detect protein S-nitrosylation...
- Species-specific inhibition of Giardia lamblia triosephosphate isomerase by localized perturbation of the homodimerSergio Enriquez-Flores
Laboratorio de Bioquímica Genética y Dirección de Investigación, Instituto Nacional de Pediatria, 04530 Mexico, DF, Mexico
Mol Biochem Parasitol 157:179-86. 2008..The effect of thiol reagents on human TIM was also assayed; it is 180-fold less sensitive than GlTIM. Collectively, the data illustrate GlTIM as a good target for drug design...
- Selective optical sensing of biothiols with Ellman's reagent: 5,5'-Dithio-bis(2-nitrobenzoic acid)-modified gold nanoparticlesKubilay Güçlü
Department of Chemistry, Faculty of Engineering, Istanbul University, Avcilar, 34320 Istanbul, Turkey
Anal Chim Acta 794:90-8. 2013..Under optimized conditions, cysteine (Cys) was quantified by the proposed assay, with a detection limit (LOD) of 0.57μM and acceptable linearity ranging from 0.4 to 29.0μM (r=0.998). ..
- The properties of the metal-thiolate clusters in recombinant mouse metallothionein-4Bin Cai
Chemical Biology Lab, Department of Chemistry, Fudan University, Shanghai, 200433, China
Protein J 24:327-36. 2005..But while reacting with EDTA, the metal-thiolate clusters in MT-4 are more stable than those of MT-1. We suppose the negative charge of the beta-domain of MT-4 prevents the EDTA attack to MT-4...
- Cellular disulfide-reducing capacity: an integrated measure of cell redox capacityJames M May
Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
Biochem Biophys Res Commun 344:1352-9. 2006..Together, these results show that alpha-lipoic acid-dependent DTNB reduction provides a simple method to assess the disulfide-reducing capacity of intact cells, especially as determined by pyridine nucleotide-disulfide oxidoreductases...
- Identification of a stable flavin-thiolate adduct in heterotetrameric sarcosine oxidaseRobert M G Hynson
Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102, USA
J Mol Biol 362:656-63. 2006..A model of the postulated TSOX adduct suggests that it is stabilized by nearby residues that may be important in the electron transferase/oxidase function of the coenzyme...
- Precision-cut tissue chips as an in vitro toxicology systemJ M Catania
Veterinary Integrative Biosciences, College of Veterinary Medicine, Texas A and M University, College Station, TX 77843, USA
Toxicol In Vitro 21:956-61. 2007..Importantly, the number of samples per organ/mouse was increased at least threefold and a significant time reduction per analysis was realized...
- Carboxymethylproline synthase (CarB), an unusual carbon-carbon bond-forming enzyme of the crotonase superfamily involved in carbapenem biosynthesisMark C Sleeman
Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford OX1 3TA, UK
J Biol Chem 279:6730-6. 2004..The implications of the results for the biosynthesis of both carbapenem-3-carboxylate and C-2/C-6-substituted carbapenems, such as thienamycin, are discussed...
- Contribution of cysteines to clathrin trimerization domain stability and mapping of light chain bindingJoel A Ybe
Department of Biology, Indiana University, Bloomington, IN 47405, USA
Traffic 4:850-6. 2003..EMBO J 2002; 21: 6072-6082; Pishvaee B, Munn A, Payne GS. EMBO J 1997; 16: 2227-2239)...
- Substrate and inhibitor specificities differ between human cytosolic and mitochondrial thioredoxin reductases: Implications for development of specific inhibitorsOliver Rackham
Western Australian Institute for Medical Research, Centre for Medical Research, University of Western Australia, Perth, WA 6000, Australia
Free Radic Biol Med 50:689-99. 2011..TrxR2 displayed strikingly lower activity with dithionitrobenzoic acid (DTNB), lipoamide, and the quinone substrate juglone compared to TrxR1, and TrxR2 could not reduce lipoic ..
- Towards creatine kinase aggregation due to the cysteine modification at the flexible active site and refolding pathwayHang Mu
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, PR China
Int J Biol Macromol 41:439-46. 2007....
- Reduction of aggregation of β-lactoglobulin during heating by dihydrolipoic acidHeni B Wijayanti
School of Agriculture and Food Sciences, The University of Queensland, Brisbane QLD 4072, Australia
J Dairy Res 80:383-9. 2013..However, the advantage of using DHLA over NEM and DTNB to lessen aggregation of β-Lg is that it is a food-grade compound which occurs naturally in milk. ..
- Intracellular adenosine triphosphate and glutathione concentrations in oocytes from first estrous, multi-estrous, and testosterone-treated giltsJ R Herrick
Department of Animal Sciences, The Ohio State University, 2027 Coffey Road, Columbus, OH 43210, USA
Anim Reprod Sci 78:123-31. 2003..Of the numerous potential markers of developmental potential, two were examined in the current study, and GSHi appeared to be useful for assessing porcine oocytes...
- A bicistronic expression system for bacterial production of authentic human interleukin-18Robert B Kirkpatrick
Department of Gene Expression, Protein Biochemistry, GlaxoSmithKline Pharmaceuticals, 709 Swedeland Rd, King of Prussia, PA 19406, USA
Protein Expr Purif 27:279-92. 2003....
- The cysteine residues in the carboxy terminal domain of tropoelastin form an intrachain disulfide bond that stabilizes a loop structure and positively charged pocketP L Brown
Department of Cell Biology, Jewish Hospital, Washington University Medical Center, St Louis, MO 63110
Biochem Biophys Res Commun 186:549-55. 1992..The side chain of a conserved lysine residue in the disulfide-bonded loop forms the top of the pocket. This positively charged pocket may define a binding site for acidic microfibrillar proteins that mediate elastic fiber assembly...
- A broadly applicable continuous spectrophotometric assay for measuring aminoacyl-tRNA synthetase activityA J Lloyd
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
Nucleic Acids Res 23:2886-92. 1995..Finally, this novel method was used to provide direct evidence for the cooperativity of tRNA and ATP binding to GlnRS...
- Purification and characterization of the bifunctional CobU enzyme of Salmonella typhimurium LT2. Evidence for a CobU-GMP intermediateG A O'Toole
Department of Bacteriology, University of Wisconsin, Madison 53706, USA
J Biol Chem 270:23560-9. 1995..In vitro experiments are consistent with previous genetic studies which had suggested that adenosyl-cobinamide was the preferred substrate of CobU, and that CobU functioned more efficiently in the absence of oxygen...
- Effect of lecithin:cholesterol acyltransferase on distribution of apolipoprotein A-IV among lipoproteins of human plasmaC L Bisgaier
J Lipid Res 28:693-703. 1987..ApoA-IV may bind to these "gaps," where the packing density of the phospholipid head groups is reduced...
- Effects of bienzyme complex formation of cysteine synthetase from escherichia coli on some properties and kineticsK Mino
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Japan
Biosci Biotechnol Biochem 64:1628-40. 2000....
- Derivatization of phytochelatins from Silene vulgaris, induced upon exposure to arsenate and cadmium: comparison of derivatization with Ellman's reagent and monobromobimaneF E Sneller
Department of Ecology and Ecotoxicology, Vrije Universiteit Amsterdam, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands
J Agric Food Chem 48:4014-9. 2000..4% and 50.2% for PC(4). This decrease is most likely due to steric hindrance. Correction of measured thiol concentration is therefore advised for better quantification of PC concentrations in plant material...
- Chemical modification of xylanases: evidence for essential tryptophan and cysteine residues at the active siteV Deshpande
Division of Biochemical Sciences, National Chemical Laboratory, Pune, India
Biochim Biophys Acta 1041:172-7. 1990..Xylan afforded complete protection against inactivation by NBS, HNBB and PHMB, indicating the involvement of tryptophan and cysteine residues at the substrate-binding region of the enzyme...
- Calorimetry Work StationSHERWIN LEHRER; Fiscal Year: 2002..iii) measure binding constants and characterize the thermodynamics of binding equilibria. ITC avoids the unavoidable perturbation of fluorescence labels and is more quantitatively than ELISA assays of binding. ..
- TROPOMYOSIN AND THE REGULATION OF MUSCLE CONTRACTIONSHERWIN LEHRER; Fiscal Year: 2009..These measurements between specific labeled amino acid residues will provide information about the dynamics of the changes between the components involved in the regulatory process. ..
- Anesthetics block neuronal voltage-gated Ca2+ currentsSLOBODAN TODOROVIC; Fiscal Year: 2009..abstract_text> ..
- ABCA1 agonist peptides for therapeutic useJOHN BIELICKI; Fiscal Year: 2008..The research is likely to have a high impact on public health, since the therapeutics will be amenable to the widespread treatment of atherosclerosis. [unreadable] [unreadable] [unreadable] [unreadable]..
- Antioxidant Interaction of Selenium and Vitamins C & EJames May; Fiscal Year: 2008..Using this approach, it will be possible to relate the crucial, mitochondrial functions of selenium and vitamins C and E to the antioxidant defenses of the whole animal. ..
- Antioxidant vitamins in models of Alzheimer's DiseaseJames May; Fiscal Year: 2008..If antioxidant vitamins ameliorate toxicity in cell and animal models of Alzheimer's disease, then oxidant stress is involved in disease progression, and antioxidant vitamin supplements may be beneficial. ..
- ApoA-I Helical Domains and Cardiovascular AgingJOHN BIELICKI; Fiscal Year: 2005..These studies are relevant for devising novel strategies to combat cardiovascular aging. ..
- Acetylcholinesterase: A Comparative Biochemical ApproachLeo Pezzementi; Fiscal Year: 2005..abstract_text> ..
- MULTIPLE CLASSES OF CA2+ CHANNELS - SENSORY TRANSMISSIONSLOBODAN TODOROVIC; Fiscal Year: 2004..abstract_text> ..
- Antioxidant Functions of Lipoic AcidJames May; Fiscal Year: 2004..Despite the widespread use of lipoic acid, these studies are necessary to establish the biological basis and rationale for the clinical use of the agent. ..
- PRESERVATION OF HDL FUNCTION DURING EARLY ATHEROGENESISJOHN BIELICKI; Fiscal Year: 2002..Moreover, the research will uncover novel mechanisms for preserving HDL/LCAT function that can be utilized therapeutically to fight the onset of cardiovascular disease. ..
- REDOX PHARMACOLOGY OF T CHANNELS IN DRG NEURONSSLOBODAN TODOROVIC; Fiscal Year: 2009..In addition, our results suggest an unexplored avenue for the development of potential novel therapies for pain control. ..