diamide

Summary

Summary: A sulfhydryl reagent which oxidizes sulfhydryl groups to the disulfide form. It is a radiation-sensitizing agent of anoxic bacterial and mammalian cells.

Top Publications

  1. ncbi Regulation of mitochondrial NADP+-dependent isocitrate dehydrogenase activity by glutathionylation
    In Sup Kil
    Department of Biochemistry, College of Natural Sciences, Kyungpook National University, Taegu 702 701, Korea
    J Biol Chem 280:10846-54. 2005
  2. ncbi Identification of a critical region in the Drosophila ryanodine receptor that confers sensitivity to diamide insecticides
    Yong Tao
    Department of Microbiology and Biotechnology, Institute of Microbiology, Chinese Academy of Sciences, 1 West Beichen Road, Chaoyang District, Beijing 100101, China
    Insect Biochem Mol Biol 43:820-8. 2013
  3. pmc The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes
    Diana Nakunst
    Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie, Universitat Bielefeld, 33501 Bielefeld, Germany
    J Bacteriol 189:4696-707. 2007
  4. pmc Genome-wide identification of genes directly regulated by the pleiotropic transcription factor Spx in Bacillus subtilis
    Tatiana Rochat
    INRA, UMR 1319 Micalis, France
    Nucleic Acids Res 40:9571-83. 2012
  5. pmc Diamide triggers mainly S Thiolations in the cytoplasmic proteomes of Bacillus subtilis and Staphylococcus aureus
    Dierk Christoph Pöther
    Institute of Microbiology1 and Institute of Pharmaceutical Biology, 2 Ernst Moritz Arndt University of Greifswald, F L Jahnstr 15, D 17487 Greifswald, Germany
    J Bacteriol 191:7520-30. 2009
  6. ncbi Regulation of quinone detoxification by the thiol stress sensing DUF24/MarR-like repressor, YodB in Bacillus subtilis
    Montira Leelakriangsak
    Department of Environmental and Biomolecular Systems, OGI School of Science and Engineering, Oregon Health and Science University, Beaverton, OR, USA
    Mol Microbiol 67:1108-24. 2008
  7. ncbi Functional analysis of sigH expression in Corynebacterium glutamicum
    Tae Hyun Kim
    Graduate School of Biotechnology, Korea University, Anam Dong, Sungbuk Ku, Seoul 136 701, Republic of Korea
    Biochem Biophys Res Commun 331:1542-7. 2005
  8. pmc Identification by redox proteomics of glutathionylated proteins in oxidatively stressed human T lymphocytes
    Maddalena Fratelli
    Mario Negri Institute for Pharmacological Research, 20157 Milan, Italy
    Proc Natl Acad Sci U S A 99:3505-10. 2002
  9. pmc Global characterization of disulfide stress in Bacillus subtilis
    Lars Ingo Ole Leichert
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, 17487 Greifswald, Germany
    J Bacteriol 185:1967-75. 2003
  10. ncbi Modulation of the expression of ryanodine receptor mRNA from Plutella xylostella as a result of diamide insecticide application
    Lina Sun
    Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China
    Gene 511:265-73. 2012

Detail Information

Publications245 found, 100 shown here

  1. ncbi Regulation of mitochondrial NADP+-dependent isocitrate dehydrogenase activity by glutathionylation
    In Sup Kil
    Department of Biochemistry, College of Natural Sciences, Kyungpook National University, Taegu 702 701, Korea
    J Biol Chem 280:10846-54. 2005
    ..HEK293 cells and intact respiring mitochondria treated with oxidants inducing GSH oxidation such as H(2)O(2) or diamide showed a decrease in IDPm activity and the accumulation of glutathionylated enzyme...
  2. ncbi Identification of a critical region in the Drosophila ryanodine receptor that confers sensitivity to diamide insecticides
    Yong Tao
    Department of Microbiology and Biotechnology, Institute of Microbiology, Chinese Academy of Sciences, 1 West Beichen Road, Chaoyang District, Beijing 100101, China
    Insect Biochem Mol Biol 43:820-8. 2013
    ..of the Drosophila C-terminus with that from Meloidogyne results in a functional RyR which lack sensitivity to diamide insecticides...
  3. pmc The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes
    Diana Nakunst
    Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie, Universitat Bielefeld, 33501 Bielefeld, Germany
    J Bacteriol 189:4696-707. 2007
    ..glutamicum cells more sensitive to heat, cold, and the presence of the thiol oxidant diamide. Transcription of the sigM gene increased under different stress conditions, including heat shock, cold shock, ..
  4. pmc Genome-wide identification of genes directly regulated by the pleiotropic transcription factor Spx in Bacillus subtilis
    Tatiana Rochat
    INRA, UMR 1319 Micalis, France
    Nucleic Acids Res 40:9571-83. 2012
    ..Three quarters of these sites were located near Sigma(A)-dependent promoters, and upon diamide treatment, the fraction of the Spx-RNAP complex increased in parallel with the number and occupancy of DNA sites...
  5. pmc Diamide triggers mainly S Thiolations in the cytoplasmic proteomes of Bacillus subtilis and Staphylococcus aureus
    Dierk Christoph Pöther
    Institute of Microbiology1 and Institute of Pharmaceutical Biology, 2 Ernst Moritz Arndt University of Greifswald, F L Jahnstr 15, D 17487 Greifswald, Germany
    J Bacteriol 191:7520-30. 2009
    ..subtilis after treatment of cells with the disulfide-generating electrophile diamide. S cysteinylation is thought to protect protein thiols against irreversible oxidation to sulfinic and sulfonic ..
  6. ncbi Regulation of quinone detoxification by the thiol stress sensing DUF24/MarR-like repressor, YodB in Bacillus subtilis
    Montira Leelakriangsak
    Department of Environmental and Biomolecular Systems, OGI School of Science and Engineering, Oregon Health and Science University, Beaverton, OR, USA
    Mol Microbiol 67:1108-24. 2008
    ..and the azoreductase encoding yvaB (azoR2) gene in response to thiol-specific stress conditions, such as diamide, catechol and 2-methylhydroquinone (MHQ)...
  7. ncbi Functional analysis of sigH expression in Corynebacterium glutamicum
    Tae Hyun Kim
    Graduate School of Biotechnology, Korea University, Anam Dong, Sungbuk Ku, Seoul 136 701, Republic of Korea
    Biochem Biophys Res Commun 331:1542-7. 2005
    ..glutamicum cells sensitive to the thiol-specific oxidant diamide. In the sigH mutant strain, the activity of thioredoxin reductase markedly decreased, suggesting that the trxB ..
  8. pmc Identification by redox proteomics of glutathionylated proteins in oxidatively stressed human T lymphocytes
    Maddalena Fratelli
    Mario Negri Institute for Pharmacological Research, 20157 Milan, Italy
    Proc Natl Acad Sci U S A 99:3505-10. 2002
    ..To this purpose, we radiolabeled cellular glutathione with (35)S, exposed T cells to oxidants (diamide or hydrogen peroxide), and performed nonreducing, two-dimensional electrophoresis followed by detection of ..
  9. pmc Global characterization of disulfide stress in Bacillus subtilis
    Lars Ingo Ole Leichert
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, 17487 Greifswald, Germany
    J Bacteriol 185:1967-75. 2003
    ..To induce disulfide stress, we used the specific thiol oxidant diamide. After addition of 1 mM diamide to an exponentially growing culture, cell growth stopped until the medium was ..
  10. ncbi Modulation of the expression of ryanodine receptor mRNA from Plutella xylostella as a result of diamide insecticide application
    Lina Sun
    Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China
    Gene 511:265-73. 2012
    ..With the development of diamide insecticides that exhibit a novel mode of action on the RyRs from Lepidoptera, research on insect RyRs has become ..
  11. ncbi Effects of cyantraniliprole, a novel anthranilic diamide insecticide, against Asian citrus psyllid under laboratory and field conditions
    Siddharth Tiwari
    Entomology and Nematology Department, Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, USA
    Pest Manag Sci 69:1066-72. 2013
    ..citri has been documented. There is an urgent need to develop and integrate novel tools for the successful management of D. citri and also to prevent the development of insecticide resistance...
  12. ncbi Reactive oxygen species induced by diamide inhibit insulin-induced ATP-sensitive potassium channel activation in cultured vascular smooth muscle cells
    Kazuaki Mawatari
    Department of Metabolism and Nutrition, University of Tokushima Graduate School, 3 18 15 Kuramoto cho, Tokushima, 770 8503, Japan
    Asia Pac J Clin Nutr 17:162-6. 2008
    ..Here we examined the effects of ROS induced by a thol-oxidizing agent, diamide, on the insulin signalling pathway and KATP channel activities in cultured VSMCs (A10 cells)...
  13. ncbi Tolerance of the mouse sperm nuclei to freeze-drying depends on their disulfide status
    Takehito Kaneko
    Institute for Biogenesis Research, Department of Anatomy and Reproductive Biology, John A Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96822, USA
    Biol Reprod 69:1859-62. 2003
    ..However, immature spermatozoa became resistant to freeze-drying after their treatment with diamide, which oxidizes free -SH groups...
  14. pmc Staphylococcus aureus NfrA (SA0367) is a flavin mononucleotide-dependent NADPH oxidase involved in oxidative stress response
    Karin Streker
    Institut für Molekulare Infektionsbiologie, Rontgenring 11, D 97070 Wurzburg, Germany
    J Bacteriol 187:2249-56. 2005
    ..Transcription was strongly induced by ethanol, diamide, and nitrofurantoin. Hydrogen peroxide induced nfrA transcription only at high concentrations...
  15. ncbi In cystic fibrosis homozygotes and heterozygotes, neutrophil apoptosis is delayed and modulated by diamide or roscovitine: evidence for an innate neutrophil disturbance
    Sandra Moriceau
    INSERM U845, Necker Hospital, Paris, France
    J Innate Immun 2:260-6. 2010
    ..b>Diamide, a direct thiol-oxidizing agent, potentiated PMN apoptosis in controls and CF patients, resulting in similar ..
  16. pmc RNA polymerase mapping during stress responses reveals widespread nonproductive transcription in yeast
    Tae Soo Kim
    Department of Biological Chemistry and Molecular Pharmacology, Harvard University, 240 Longwood Avenue, Boston, MA 02115, USA
    Genome Biol 11:R75. 2010
    ..However, changes in mRNA abundance reflect the combined effect of changes in RNA production, processing, and degradation, and thus, mRNA levels provide an occluded view of transcriptional regulation...
  17. pmc Comparison of gene expression signatures of diamide, H2O2 and menadione exposed Aspergillus nidulans cultures--linking genome-wide transcriptional changes to cellular physiology
    Istvan Pocsi
    Department of Microbiology and Biotechnology, Faculty of Science, University of Debrecen, PO Box 63, H 4010 Debrecen, Hungary
    BMC Genomics 6:182. 2005
    ....
  18. pmc Dual negative control of spx transcription initiation from the P3 promoter by repressors PerR and YodB in Bacillus subtilis
    Montira Leelakriangsak
    OGI School of Science and Engineering, Oregon Health and Science University, Beaverton, OR 97229, USA
    J Bacteriol 189:1736-44. 2007
    ..Induction of spx transcription in response to treatment with the thiol-specific oxidant diamide is the result of transcription initiation at the P(3) promoter located upstream of the spx coding sequence...
  19. ncbi Use of diamide-acridine orange fluorescence staining to detect aberrant protamination of human-ejaculated sperm nuclei
    Haruo Katayose
    Department of Obstetrics and Gynecology, School of Medicine, Fukushima Medical University, Fukushima, Japan
    Fertil Steril 79:670-6. 2003
    ..To investigate the influence of human sperm nuclear chromatin on fertilization...
  20. ncbi Proteomic signatures uncover thiol-specific electrophile resistance mechanisms in Bacillus subtilis
    Haike Antelmann
    Institute of Microbiology, Ernst Moritz Arndt University of Greifswald, F L Jahn Str 15, D 17487 Greifswald, Germany
    Expert Rev Proteomics 5:77-90. 2008
    ..Catechol, 2-MHQ and diamide displayed a common mode of action, as revealed by the induction of the thiol-specific oxidative stress response...
  21. pmc Genomic expression programs in the response of yeast cells to environmental changes
    A P Gasch
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5428, USA
    Mol Biol Cell 11:4241-57. 2000
    ..temperature shocks, hydrogen peroxide, the superoxide-generating drug menadione, the sulfhydryl-oxidizing agent diamide, the disulfide-reducing agent dithiothreitol, hyper- and hypo-osmotic shock, amino acid starvation, nitrogen ..
  22. ncbi The sigmaR regulon of Streptomyces coelicolor A32 reveals a key role in protein quality control during disulphide stress
    Dimitris Kallifidas
    Department of Chemistry and Biochemistry, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK
    Microbiology 156:1661-72. 2010
    b>Diamide is an artificial disulphide-generating electrophile that mimics an oxidative shift in the cellular thiol-disulphide redox state (disulphide stress)...
  23. ncbi Importance of ligand bioactive conformation in the discovery of potent indole-diamide inhibitors of the hepatitis C virus NS5B
    Steven R LaPlante
    Department of Chemistry, Boehringer Ingelheim Canada Ltd, 2100 Cunard St, Laval, Quebec, Canada, H7S2G5
    J Am Chem Soc 132:15204-12. 2010
    ....
  24. ncbi The redox-sensing regulator YodB senses quinones and diamide via a thiol-disulfide switch in Bacillus subtilis
    Bui Khanh Chi
    Institute for Microbiology, Ernst Moritz Arndt University of Greifswald, Greifswald, Germany
    Proteomics 10:3155-64. 2010
    ..the azoreductase AzoR1, the nitroreductase YodC and the redox-sensing regulator Spx in response to quinones and diamide in Bacillus subtilis...
  25. ncbi Expression of macrophage inflammatory protein 1 alpha in the endothelial cells exposed to diamide
    Limin Yang
    Department of Pathology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030
    J Huazhong Univ Sci Technolog Med Sci 23:219-22, 233. 2003
    In order to study whether the endothelial cells (ECs) with lipid peroxidation induced by diamide can express and secrete macrophage inflammatory protein 1 alpha (MIP-1 alpha), the expression of MIP-1 alpha protein in the cells was ..
  26. ncbi Ineffective GSH regeneration enhances G6PD-knockdown Hep G2 cell sensitivity to diamide-induced oxidative damage
    Li Ping Gao
    Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, People s Republic of China
    Free Radic Biol Med 47:529-35. 2009
    ..Hep G2 cells expressing shRNA against G6PD (Gi) were more susceptible to diamide-induced cytotoxicity than control cells expressing scrambled control shRNA (Sc)...
  27. ncbi A novel NADPH:diamide oxidoreductase activity in arabidopsis thaliana P1 zeta-crystallin
    J Mano
    Research Institute for Food Science, Kyoto University, Uji, Japan
    Eur J Biochem 267:3661-71. 2000
    ..of Arabidopsis thaliana, known to confer tolerance toward the oxidizing drug 1,1'-azobis(N, N-dimethylformamide) (diamide) to yeast [Babiychuk, E., Kushnir, S., Belles-Boix, E., Van Montagu, M. & Inzé, D. (1995) J. Biol. Chem...
  28. ncbi Effector-induced Syk-mediated phosphorylation in human erythrocytes
    Luciana Bordin
    Department of Biological Chemistry, University of Padua, Viale G Colombo 3, 35121 Padua, Italy
    Biochim Biophys Acta 1745:20-8. 2005
    ..tyrosine phosphorylated following treatment of intact cells with protein tyrosine phosphatase inhibitors such as diamide, pervanadate, vanadate, or N-ethylmaleimide (NEM)...
  29. pmc Requirement of the zinc-binding domain of ClpX for Spx proteolysis in Bacillus subtilis and effects of disulfide stress on ClpXP activity
    Ying Zhang
    Environmental and Biomolecular Systems, OGI School of Science and Engineering, Oregon Health and Science University, 20000 NW Walker Rd, Beaverton, OR 97006, USA
    J Bacteriol 189:7669-80. 2007
    ..The effect of disulfide stress on ClpXP activity was examined using the thiol-specific oxidant diamide. ClpXP-catalyzed degradation of either Spx or a green fluorescent protein derivative bearing an SsrA tag ..
  30. ncbi Arabidopsis thaliana NADPH oxidoreductase homologs confer tolerance of yeasts toward the thiol-oxidizing drug diamide
    E Babiychuk
    Laboratorium voor Genetica, Universiteit Gent, Belgium
    J Biol Chem 270:26224-31. 1995
    ..approximately 10(5) primary transformants were subjected to a tolerance screen toward the thiol-oxidizing drug diamide, which depletes the reduced glutathione in the cell. Four types of Arabidopsis cDNAs were isolated...
  31. pmc Flexibility in the receptor-binding domain of the enzymatic colicin E9 is required for toxicity against Escherichia coli cells
    Christopher N Penfold
    School of Molecular Medical Sciences and Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University of Nottingham, Nottingham NG7 2RD, United Kingdom
    J Bacteriol 186:4520-7. 2004
    ..On oxidation with diamide, no significant biological activity was seen with the ColE9 L359C-F412C and the ColE9 Y324C-L447C mutant proteins;..
  32. ncbi Regulation of cAMP-dependent protein kinase activity by glutathionylation
    Kenneth M Humphries
    Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, The University of California, San Diego, La Jolla, California 92093 0654, USA
    J Biol Chem 277:43505-11. 2002
    ..In vitro studies demonstrated the susceptibility of kinase activity to the sulfhydryl oxidant diamide, which inhibited by promoting an intramolecular disulfide bond between cysteines 199 and 343...
  33. ncbi Oxidative stress inhibits ionomycin-mediated cell death in cortical neurons
    Adrian T McCollum
    Department of Physiology, University of Kentucky, Lexington, Kentucky 40536, USA
    J Neurosci Res 76:104-9. 2004
    ..To test this hypothesis, cultured rat cortical neurons were treated with the oxidizing agent diamide or doxorubicin in the presence or absence of the calcium ionophore ionomycin...
  34. ncbi Glycogenolysis is directed towards ascorbate synthesis by glutathione conjugation
    Tom S Chan
    Faculty of Pharmacy, University of Toronto, 19 Russell St Rm 522, Toronto, Ont, Canada M5S 2S2
    Biochem Biophys Res Commun 317:149-56. 2004
    ..These data suggest that GSH depletion can stimulate ascorbate synthesis independently of glucose synthesis and that hepatocytes can direct glycogenolysis towards ascorbate synthesis during GSH conjugation...
  35. ncbi Regulation of annexin A2 by reversible glutathionylation
    Jennifer F Caplan
    Cancer Biology Research Group, Department of Biochemistry, University of Calgary, Calgary, Alberta T2N 4N1, Canada
    J Biol Chem 279:7740-50. 2004
    ..Oxidation of AIIt by diamide resulted in a time- and concentration-dependent loss of the ability of AIIt to interact with phospholipid ..
  36. ncbi Dual effect of oxidative stress on NF-kappakB activation in HeLa cells
    Mi Sun Byun
    Department of Biochemistry, College of Medicine, The Catholic University of Korea, 505 Banpo Dong, Seocho Gu, Seoul 137 701, Korea
    Exp Mol Med 34:332-9. 2002
    ..such conflicting effect of oxidative stress on NF- kappakB activation, HeLa cells were incubated with H(2)O(2) or diamide and TNF-induced expression of NF-kappaB reporter gene was measured...
  37. ncbi Actin S-glutathionylation: evidence against a thiol-disulphide exchange mechanism
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Milan, Italy
    Free Radic Biol Med 35:1185-93. 2003
    ..Instead, actin with the exposed cysteine thiol activated by diamide or 5,5'-dithiobis(2-nitrobenzoic acid) reacts with physiological levels of GSH, incorporating about 0...
  38. ncbi Visualization of the compartmentalization of glutathione and protein-glutathione mixed disulfides in cultured cells
    Therese Söderdahl
    Division of Biochemical Toxicology, Karolinska Institutet, Stockholm Sweden
    FASEB J 17:124-6. 2003
    ..initial visualization of the compartmentalization of protein-GSH mixed disulfides formed in A549 cells exposed to diamide. Discontinuous staining was again evident, with heavy staining in membrane blebs and in the nuclear region...
  39. ncbi Targeted disruption of specific steps of the ubiquitin-proteasome pathway by oxidation in lens epithelial cells
    Mathew R Hosler
    Department of Biochemistry and Molecular Biology, 185 South Orange Avenue, Newark, NJ 07101, USA
    Int J Biochem Cell Biol 35:685-97. 2003
    ..several steps of the ubiquitin-proteasome pathway with an alternative agent: the thiol-specific oxidant, diamide. The 20S core proteasome, de-ubiquitinating, and ATP-dependent 26S proteasome activities all showed decreases 10 ..
  40. pmc Failure to increase glucose consumption through the pentose-phosphate pathway results in the death of glucose-6-phosphate dehydrogenase gene-deleted mouse embryonic stem cells subjected to oxidative stress
    Stefania Filosa
    IGB Adriano Buzzati Traverso CNR, Via G Marconi 12, 80125 Napoli, Italy
    Biochem J 370:935-43. 2003
    ..Moreover, bacterial G6PD can substitute for the human enzyme, strongly suggesting that a relatively simple mechanism of enzyme kinetics underlies this phenomenon...
  41. ncbi MRP1-transfected cells do not show increased resistance against oxidative stress
    Aneta Balcerczyk
    Department of Molecular Biophysics, University of Lodz, 90 237 Lodz, Poland
    Free Radic Res 37:189-95. 2003
    ..Cells overexpressing MRP1 did not show increased resistance to tert-butyl hydroperoxide, diamide, paraquat, menadione, dichromate and carmustine as estimated by cell survival and DNA damage assessed by comet ..
  42. ncbi Thiol oxidation-mediated cell death in Aplysia cultured sensory neurons
    Deok Jin Chang
    National Research Laboratory, Institute of Molecular Biology and Genetics, School of Biological Sciences, College of Natural Sciences, Seoul National University, San 56 1 Silim dong Kwanak gu, Seoul 151 742, South Korea
    Brain Res 1007:71-7. 2004
    ..In this study, we found that a thiol oxidant, diamide, induced morphological changes such as cell swelling, membrane blebbing, and chromatin condensation in Aplysia ..
  43. ncbi Redox sensitivity of the ryanodine receptor interaction with FK506-binding protein
    Spyros Zissimopoulos
    Wales Heart Research Institute, Department of Cardiology, Cardiff University School of Medicine, Cardiff CF14 4XN, United Kingdom
    J Biol Chem 282:6976-83. 2007
    ..6, we found that the sulfydryl-oxidizing agents, H(2)O(2) and diamide, result in diminished RyR2-FKBP12.6 binding...
  44. pmc Targeted disruption of the glutaredoxin 1 gene does not sensitize adult mice to tissue injury induced by ischemia/reperfusion and hyperoxia
    Ye Shih Ho
    Institute of Environmental Health Sciences and Department of Biochemistry and Molecular Biology, Wayne State University, Detroit, MI 48201, USA
    Free Radic Biol Med 43:1299-312. 2007
    ..diquat and paraquat, but they were not more susceptible to cell death induced by hydrogen peroxide (H(2)O(2)) and diamide. A deficiency in Grx1 also sensitized MEFs to protein S-glutathionylation in response to H(2)O(2) treatment and ..
  45. pmc Mitochondrial thioredoxin in regulation of oxidant-induced cell death
    Yan Chen
    Department of Medicine, Division of Pulmonary, Allergy and Critical Care Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA
    FEBS Lett 580:6596-602. 2006
    ..Results showed that mtTrx was oxidized after exposure to peroxides and diamide. Overexpression of mtTrx protected against diamide-induced oxidation and cytotoxicity...
  46. ncbi Transcriptional activation of dehalorespiration. Identification of redox-active cysteines regulating dimerization and DNA binding
    Stelian M Pop
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Biol Chem 281:26382-90. 2006
    ..When incubated with oxygen or diamide, CprK undergoes inactivation; subsequent treatment with dithiothreitol restores activity...
  47. ncbi Oxidized forms of glutathione in peripheral blood as biomarkers of oxidative stress
    Ranieri Rossi
    Department of Neuroscience, Pharmacology Section, University of Siena, Siena, Italy
    Clin Chem 52:1406-14. 2006
    ..We evaluated the importance of correct sample handling during the preanalytical phase for GSH, GSSG, and PSSG measurement...
  48. ncbi Reducing and oxidizing agents sensitize heat-activated vanilloid receptor (TRPV1) current
    Klara Susankova
    Department of Cellular Neurophysiology, Institute of Physiology AS CR, Videnska 1083, 142 20 Prague 4, Czech Republic
    Mol Pharmacol 70:383-94. 2006
    ..The sensitizing effects of the membrane-permeable oxidizing agents diamide (1 mM), chloramine-T (1 mM), and the copper-o-complex (100:400 microM) were not reversed by washout, consistent ..
  49. ncbi Enhanced oxidative stress by L-ascorbic acid within cells challenged by hydrogen peroxide
    Yoko Inai
    Department of Biochemistry, Wakayama Medical University, Kimiidera 811 1, Wakayama 641 0012, Japan
    J Nutr Sci Vitaminol (Tokyo) 51:398-405. 2005
    ....
  50. ncbi Band 3 tyr-phosphorylation in normal and glucose-6-phospate dehydrogenase-deficient human erythrocytes
    Luciana Bordin
    Department of Biological Chemistry, University of Padova, Viale G Colombo, 3, 35121, Padova, Italy
    Mol Membr Biol 22:411-20. 2005
    ..show that this sensitivity is connected to an early membrane band 3 Tyr-phosphorylation in the presence of diamide. However, since both Syk, and Lyn kinases, and SHP-2 phosphatase, mostly implicated in the band 3 P-Tyr level ..
  51. ncbi Fluorescence thiol modification assay: oxidatively modified proteins in Bacillus subtilis
    Falko Hochgräfe
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat Greifswald, F L Jahn Strasse 15, D 17487 Greifswald, Germany
    Mol Microbiol 58:409-25. 2005
    ..To detect proteins that are particularly sensitive to oxidative stress we exposed growing B. subtilis cells to diamide, hydrogen peroxide or to the superoxide generating agent paraquat...
  52. ncbi A novel thiol oxidation-based mechanism for adriamycin-induced cell injury in human macrophages
    Reto Asmis
    Division of Cardiovascular Medicine and Graduate Center for Nutritional Sciences, University of Kentucky, USA
    FASEB J 19:1866-8. 2005
    ..Macrophages treated with either adriamycin or the thiol oxidant diamide showed elevated levels of glutathione disulfide and increased protein-S-glutathionylation prior to cell injury, ..
  53. ncbi Biphasic lindane-induced oxidation of glutathione and inhibition of gap junctions in myometrial cells
    Rita Loch Caruso
    Toxicology Program, Department of Environmental Health, University of Michigan, 1420 Washington Heights, Ann Arbor, Michigan 48109 2029, USA
    Toxicol Sci 86:417-26. 2005
    ..Furthermore, treatments that promoted accumulation of GSSG (50 microM diamide and 25 microM 1,3-bis(2-chloroethyl)-1-nitrosourea [BCNU]) inhibited Lucifer yellow dye transfer between ..
  54. ncbi S-glutathionylation in human platelets by a thiol-disulfide exchange-independent mechanism
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Via Celoria 26, I 20133 Milan, Italy
    Free Radic Biol Med 38:1501-10. 2005
    ..in vitro by exposure of human platelets to the thiol-specific oxidant azodicarboxylic acid-bis-dimethylamide (diamide)...
  55. pmc Thioredoxin reductase is essential for thiol/disulfide redox control and oxidative stress survival of the anaerobe Bacteroides fragilis
    Edson R Rocha
    Department of Microbiology and Immunology, East Carolina University Brody School of Medicine, 600 Moye Blvd, Greenville, NC 27834, USA
    J Bacteriol 189:8015-23. 2007
    ..Expression of the B. fragilis trxB gene was induced following treatment with diamide or H(2)O(2) or exposure to oxygen. This inducible trxB expression was OxyR independent...
  56. ncbi Regulation of the catalytic activity and structure of human thioredoxin 1 via oxidation and S-nitrosylation of cysteine residues
    Seyed Isaac Hashemy
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    J Biol Chem 283:21890-8. 2008
    ..Human Trx1 has also three structural Cys residues in positions 62, 69, and 73 that upon diamide oxidation induce a second Cys(62)-Cys(69) disulfide as well as dimers and multimers...
  57. ncbi An altered oxidant defense system in red blood cells affects their ability to release nitric oxide-stimulating ATP
    Jamie Carroll
    Department of Chemistry, Wayne State University, 5101 Cass Ave, Detroit, MI 48202, USA
    Mol Biosyst 2:305-11. 2006
    ..These data suggest that the antioxidant status of erythrocytes is a critical determinant in the ability of these cells to release ATP, a known nitric oxide stimulus...
  58. pmc Complex I within oxidatively stressed bovine heart mitochondria is glutathionylated on Cys-531 and Cys-704 of the 75-kDa subunit: potential role of CYS residues in decreasing oxidative damage
    Thomas R Hurd
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, United Kingdom
    J Biol Chem 283:24801-15. 2008
    ..In mitochondria incubated with the thiol oxidant diamide, complex I is only glutathionylated on the 75-kDa subunit...
  59. pmc Human p53 is inhibited by glutathionylation of cysteines present in the proximal DNA-binding domain during oxidative stress
    Chinavenmeni S Velu
    Anticancer Resistance Research Group, Department of Pharmaceutical Sciences, Texas Tech University Health Sciences Center, Amarillo, Texas 79106, USA
    Biochemistry 46:7765-80. 2007
    ....
  60. ncbi Glutathione might exert an important function in caerulein-stimulated amylase release in isolated rat pancreatic acini
    Honggang Yu
    Division of Gastroenterology, Hepatology, and Infectious Diseases, Department of Medicine, Heinrich Heine University of Duesseldorf, Duesseldorf, Germany
    Pancreas 24:53-62. 2002
    ..The effect of different modes of thiol depletion on pancreatic exocrine secretory function and potential mechanisms of interference with the secretory process in pancreatic acinar cells were investigated...
  61. pmc A glutathione reductase mutant of yeast accumulates high levels of oxidized glutathione and requires thioredoxin for growth
    E G Muller
    Department of Biochemistry, University of Washington, Seattle 98195 7350, USA
    Mol Biol Cell 7:1805-13. 1996
    ..In addition, the glr1 delta mutant shows increased sensitivity to the thiol oxidant diamide. The sensitivity to diamide was suppressed by deletion of the TRX2 gene...
  62. ncbi Expression of a glutamate decarboxylase homologue is required for normal oxidative stress tolerance in Saccharomyces cerevisiae
    S T Coleman
    Department of Physiology and Biophysics, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 276:244-50. 2001
    ..bearing the glutamate decarboxylase gene (GAD1) increases resistance to two different oxidants, H(2)O(2) and diamide, in cells that contain an intact glutamate catabolic pathway. Structural similarity of the S...
  63. pmc Regulation of yAP-1 nuclear localization in response to oxidative stress
    S Kuge
    Institute of Medical Science, University of Tokyo, Minato ku, Japan
    EMBO J 16:1710-20. 1997
    ..We suggest therefore, that these cysteine residues are important in sensing the redox state of the cell and hence regulating yAP-1 activity...
  64. ncbi S-cysteinylation is a general mechanism for thiol protection of Bacillus subtilis proteins after oxidative stress
    Falko Hochgräfe
    Institute for Microbiology, Ernst Moritz Arndt University Greifswald, D 17487 Greifswald, Germany
    J Biol Chem 282:25981-5. 2007
    ..having redox-sensitive thiols are modified by cysteine in response to treatment with the thiol-specific oxidant diamide. By means of multidimensional shotgun proteomics, the sites of S-cysteinylation for six proteins could be ..
  65. ncbi Different metabolizing ability of thiol reactants in human and rat blood: biochemical and pharmacological implications
    R Rossi
    Department of Neuroscience, Pharmacology Unit, Via A. Moro 4, University of Siena, Italy
    J Biol Chem 276:7004-10. 2001
    ..protein mixed disulfides were produced in rat but not in human erythrocytes by treatment with hydroperoxides or diamide. The disappearance of all forms of glutathione (reduced, disulfide, protein mixed disulfide) was induced by ..
  66. ncbi GSH depletion, K-Cl cotransport, and regulatory volume decrease in high-K/high-GSH dog red blood cells
    H Fujise
    Laboratory of Pathobiochemistry, School of Veterinary Medicine, Azabu University, Fuchinobe, Sagamihara, Kanagawa 229, Japan
    Am J Physiol Cell Physiol 281:C2003-9. 2001
    ..chemical reactions [nitrite (NO(2))-mediated oxidation, diazene dicarboxylic acid bis-N,N-dimethylamide (diamide)-induced dithiol formation, and glutathione S-transferase (GST)-catalyzed conjugation of GSH with 1-chloro-2,4-..
  67. ncbi Induction of mild intracellular redox imbalance inhibits proliferation of CaCo-2 cells
    T Noda
    Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130-3932, USA
    FASEB J 15:2131-9. 2001
    ..Low concentrations of diamide plus 1,3-bis(2 chloroethyl)-1-nitrosourea (BCNU) increased intracellular GSSG and decreased GSH and the GSH:GSSG ..
  68. ncbi Responses of thiols to an oxidant challenge: differences between blood and tissues in the rat
    F Giannerini
    Department of Neuroscience, Pharmacology Unit, University of Siena, Via A. Moro 4, 53100, Siena, Italy
    Chem Biol Interact 134:73-85. 2001
    Treatment of rats with diamide (100 mg/kg i.p.) altered the thiol components of the blood to a very different extent than in tissues (liver, kidney, lung, spleen, heart and testis)...
  69. ncbi Resolution, detection, and characterization of redox conformers of human HSF1
    D J Manalo
    Graduate Program in Cell and Developmental Biology and Department of Cell Biology and Neuroscience, Rutgers State University, Piscataway, New Jersey 08854-8082, USA
    J Biol Chem 276:23554-61. 2001
    ..We showed that diamide, a reagent that promotes disulfide bond formation, caused a loss of immunorecognition of the monomeric hHSF1 ..
  70. ncbi Modulation of ICl(Ca) in vascular smooth muscle cells by oxidizing and cysteine-reactive reagents
    Iain A Greenwood
    Department of Pharmacology and Clinical Pharmacology, Cardiovascular Research Group, St Georges Hospital Medical School, London, UK
    Pflugers Arch 443:473-82. 2002
    ..through voltage-dependent Ca2+ channels was markedly and irreversibly enhanced by the non-specific oxidant, diamide (10-200 microM). Diamide also prolonged the decay of both currents...
  71. ncbi Protein glutathionylation: coupling and uncoupling of glutathione to protein thiol groups in lymphocytes under oxidative stress and HIV infection
    Pietro Ghezzi
    Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA
    Mol Immunol 38:773-80. 2002
    ..Treating the cells with the oxidant diamide induces thiolation of a series of proteins that can be detected by 2D electrophoresis when 35S-cysteine is used ..
  72. ncbi Apoptosis in mitotic competent undifferentiated cells is induced by cellular redox imbalance independent of reactive oxygen species production
    Erin K Pias
    Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130 3932, USA
    FASEB J 16:781-90. 2002
    ..Cells grown in standard DMEM containing 25 mM glucose were treated with diamide, a thiol oxidant, at a concentration that did not generate ROS...
  73. ncbi Role of the extracytoplasmic-function sigma factor sigma(H) in Mycobacterium tuberculosis global gene expression
    Riccardo Manganelli
    TB Center, The Public Health Research Institute at the International Center for Public Health, Newark, NJ 07103 3506, USA
    Mol Microbiol 45:365-74. 2002
    ..and its involvement in the global regulation of the response to heat shock and the thiol-specific oxidizing agent diamide. We identified 48 genes whose expression increased after exposure of M...
  74. ncbi [The role of thiol oxidants in inhibition of pancreatic exocrine secretory function and glutathione]
    Honggang Yu
    Department of Gastroenterology, Renmin Hospital, Wuhan University, Wuhan 430060, China
    Zhonghua Nei Ke Za Zhi 41:306-9. 2002
    ..Glutathione depletion has been described in all form of acute pancreatitis. This study evaluated the effect of glutathione depletion on pancreatic exocrine secretory function and explored the potential mechanisms...
  75. pmc Non-adrenergic, non-cholinergic relaxation of the bovine retractor penis muscle: role of S-nitrosothiols
    X Liu
    Department of Pharmacology, University of Glasgow
    Br J Pharmacol 111:1287-95. 1994
    ..2. Treatment of BRP muscle with either of two sulphydryl inactivating agents, diamide (1 mM) and N-ethylmaleimide (0...
  76. ncbi Evidence that protein disulfide isomerase (PDI) is involved in DNA-nuclear matrix anchoring
    Robert P VanderWaal
    Washington University School of Medicine, Division of Radiation and Cancer Biology, Department of Radiation Oncology, St Louis, Missouri 63108, USA
    J Cell Biochem 85:689-702. 2002
    ..Both effects on DNA supercoiling ability were reversed by diamide suggesting that they are dependent on the oxidation state of the protein thiols...
  77. ncbi Hydrogen peroxide and redox modulation sensitize primary mouse hepatocytes to TNF-induced apoptosis
    Derick Han
    University of Southern California Research Center for Liver Diseases, Keck School of Medicine, University of Southern California, 2011 Zonal Avenue, Los Angeles, CA 90089 9121, USA
    Free Radic Biol Med 41:627-39. 2006
    ..Redox changes seem to be important in sensitizing primary hepatocytes, because diamide, a thiol-oxidizing agent, and 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), an inhibitor of GSSG reductase, also ..
  78. pmc Heterogeneity of the neuropeptide Y (NPY) contractile and relaxing receptors in horse penile small arteries
    Dolores Prieto
    Departamento de Fisiologia, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain
    Br J Pharmacol 143:976-86. 2004
    ..NPY(3-36), the compound 1229U91 (Ile-Glu-Pro-Dapa-Tyr-Arg-Leu-Arg-Tyr-NH2, cyclic(2,4')diamide) and eventually NPY(13-36) relaxed penile small arteries...
  79. ncbi The relaxant activity of 4,7-dimethyl-1,2,5-oxadiazolo[3,4-d]-pyridazine 1,5,6-trioxide in the mouse corpus cavernosum
    Cemil Gocmen
    Department of Pharmacology, Cukurova University Medical School, Adana, Turkey
    J Pharmacol Exp Ther 316:753-61. 2006
    ..Thiol alkylation agent N-ethylmaleimide significantly enhanced FPTO-induced relaxation, and thiol-modifying agent diamide inhibited relaxation to FPTO...
  80. pmc Formation of disulfide-linked complexes between the three minor envelope glycoproteins (GP2b, GP3, and GP4) of equine arteritis virus
    Roeland Wieringa
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, The Netherlands
    J Virol 77:6216-26. 2003
    ..Furthermore, the conversion was almost instantaneous in the presence of the thiol oxidant diamide. In contrast, the sulfhydryl-modifying agent N-ethylmaleimide inhibited the formation of disulfide-bonded GP(2b)/..
  81. ncbi The use of bioluminescence resonance energy transfer 2 to study neuropeptide Y receptor agonist-induced beta-arrestin 2 interaction
    Magnus M Berglund
    Eli Lilly and Co, Lilly Corporate Center, Indianapolis, IN 46285, USA
    J Pharmacol Exp Ther 306:147-56. 2003
    ..at the Y4 receptor, the Y4 agonist 1229U91 [(Ile,Glu,Pro,Dpr,Tyr,Arg, Leu,Arg,Try-NH2)-2-cyclic(2,4'),(2',4)-diamide] was unable to provoke the same maximal response as human PP, suggesting that 1229U91 is a partial agonist...
  82. pmc Oxidative stress inactivates cobalamin-independent methionine synthase (MetE) in Escherichia coli
    Elise R Hondorp
    Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan, USA
    PLoS Biol 2:e336. 2004
    ..Moreover, MetE is completely oxidized in wild-type E. coli treated with the thiol-oxidizing agent diamide; reduced enzyme reappears just prior to the cells resuming normal growth. We argue that for E...
  83. ncbi Reactions of aromatic N-heterocycles with d0fn-metal alkyl complexes supported by chelating diamide ligands
    Paula L Diaconescu
    Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, USA
    Acc Chem Res 43:1352-63. 2010
    ..The comparison between yttrium and lutetium benzyl complexes supported by ferrocene- or pyridine-diamide ligands indicated that similar reactions occurred for specific substrates (1-methylimidazole, 2-picoline, and ..
  84. pmc Novel application of S-nitrosoglutathione-Sepharose to identify proteins that are potential targets for S-nitrosoglutathione-induced mixed-disulphide formation
    P Klatt
    Department of Estructura y Función de Proteínas, Centro de Investigaciones Biologicas, Instituto Reina Sofía de Investigaciones Nefrológicas, Consejo Superior de Investigaciones Cientificas, Velazquez 144, E 28006 Madrid, Spain
    Biochem J 349:567-78. 2000
    ..to this matrix correlated well with the susceptibility of the investigated proteins to undergo GSNO- but not diamide-induced mixed-disulphide formation in vitro...
  85. ncbi Redox pacing of proteome turnover: influences of glutathione and ketonemia
    Thomas D Lockwood
    Department of Pharmacology, School of Medicine, Wright State University, Cox Bldg, 3525 Southern Blvd, Kettering, OH 45429, USA
    Arch Biochem Biophys 417:183-93. 2003
    ..More than 75% of myocardial protein degradation was inhibited and defined by infusion of diamide (100 microM) under constant physiologic concentrations of complete amino acids...
  86. ncbi Reverse genetic analysis of the glutathione metabolic pathway suggests a novel role of PHGPX and URE2 genes in aluminum resistance in Saccharomyces cerevisiae
    U Basu
    Department of Biological Sciences, University of Alberta, T6G 2E9, Edmonton, Alberta, Canada
    Mol Genet Genomics 271:627-37. 2004
    ..phgpx3Delta and phgpx1Delta/2Delta/3Delta mutants were sensitive to oxidative stress (exposure to H(2)O(2) or diamide). Lipid peroxidation was also increased in the phgpx1Delta/2Delta/3Delta mutant compared to the parental strain...
  87. ncbi Induction of heme-oxygenase 1 inhibits endothelial cell activation by endotoxin and oxidant stress
    Eileen M Bulger
    Department of Surgery, University of Washington, Seattle, WA, USA
    Surgery 134:146-52. 2003
    ..We hypothesized that the induction of HO-1 protein would attenuate the proinflammatory response of endothelial cells to lipopolysaccharide and oxidant stress...
  88. ncbi Ischemia-elicited oxidative modulation of Ca2+/calmodulin-dependent protein kinase II
    Pavan K Shetty
    Developmental Neurobiology Program, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 283:5389-401. 2008
    ..Treatment of mouse brain synaptosomes with H2O2, diamide, and sodium nitroprusside caused aggregation of CaMKII through formation of disulfide and non-disulfide linkages, ..
  89. pmc Glucose regulation of hydroperoxide metabolism in rat intestinal cells. Stimulation of reduced nicotinamide adenine dinucleotide phosphate supply
    T Y Aw
    Department of Physiology and Biophysics, Louisiana State University Medical Center, Shreveport 71130
    J Clin Invest 94:2426-34. 1994
    ..The NADPH supply rate was quantified by controlled infusions of diamide, a thiol oxidant. At diamide infusion of 0...
  90. ncbi Protein thiol modifications of human red blood cells treated with t-butyl hydroperoxide
    C K Lii
    Department of Nutrition, Chung Shan Medical College, Taichung, Taiwan, ROC
    Biochim Biophys Acta 1336:147-56. 1997
    ..As red blood samples were treated with diamide or GSSG, in addition to the mixed disulfides observed in t-BH-treated cells, additional hemoglobin-GSH mixed ..
  91. ncbi The mitochondrial carnitine carrier: characterization of SH-groups relevant for its transport function
    C Indiveri
    Department of Pharmaco Biology, University of Bari, Italy
    Biochim Biophys Acta 1140:53-8. 1992
    ..of consecutive additions of various reagents including N-ethylmaleimide, mercurials, Cu(2+)-phenanthroline and diamide on the transport function revealed the presence of at least two different classes of SH-groups...
  92. ncbi The bZip transcription factor Cgap1p is involved in multidrug resistance and required for activation of multidrug transporter gene CgFLR1 in Candida glabrata
    Kuang Hua Chen
    Clinical Mycology Section, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, Maryland 20892, USA
    Gene 386:63-72. 2007
    ..suppressed the drug sensitivity of two Yap1p-regulated transporter mutants, Deltaatr1 and Deltaflr1, to diamide, 4-NQO, and cadmium...
  93. ncbi Curative activity of insecticides on Rhagoletis pomonella (Diptera: Tephritidae) in apples
    John C Wise
    Department of Entomology, Michigan State University, East Lansing, MI 48824, USA
    J Econ Entomol 102:1884-90. 2009
    ..The pyrethroid esfenvalerate, oxadiazine indoxacarb, anthranilic diamide chlorantraniliprole, and spinosyns spinosad and spinetoram did not show activity on apple maggot eggs and larvae ..
  94. ncbi Molecular recognition of synthetic siderophore analogues: a study with receptor-deficient and fhu(A-B) deletion mutants of Escherichia coli
    M Gaspar
    Centro de Quimica Estrutural, Instituto Superior Tecnico, Lisboa, Portugal
    Biometals 12:209-18. 1999
    ..In comparison with other polyamino-polyhydroxamate ligands studied, this dihydroxamate ligand has an extra diamide backbone that could be important for the interaction with the receptors or with FhuD...
  95. ncbi Poly(ADP-ribose) glycohydrolase activity mediates post-traumatic inflammatory reaction after experimental spinal cord trauma
    Salvatore Cuzzocrea
    Institute of Pharmacology, School of Medicine, University of Messina, Torre Biologica, Policlinico Universitario Via C Valeria, Gazzi, 98100 Messina, Italy
    J Pharmacol Exp Ther 319:127-38. 2006
    ..gene in mice or the pharmacological inhibition of PARG with GPI 16552 [N-bis-(3-phenyl-propyl)9-oxo-fluorene-2,7-diamide] (40 mg/kg i.p...
  96. ncbi Wild plants, Andropogon virginicus L. and Miscanthus sinensis Anders, are tolerant to multiple stresses including aluminum, heavy metals and oxidative stresses
    Bunichi Ezaki
    Research Institute for Bioresources, Okayama University, 2 20 1, Chuo, Kurashiki, Okayama 710 0046, Japan
    Plant Cell Rep 27:951-61. 2008
    ..The two high Al tolerant plants, Andropogon and/or Miscanthus, showed tolerance to Cr, Zn, diamide or hydrogen peroxide, suggesting common tolerance mechanisms among the tested stresses...
  97. ncbi 99mTc renal tubular function agents: current status
    D Eshima
    Emory University School of Medicine, Atlanta, GA
    Semin Nucl Med 20:28-40. 1990
    ..The search for a suitable 99mTc renal tubular function agent has focused on the diamide dithiolate (N2S2), the paraaminohippuric iminodiacetic acid (PAHIDA), and the triamide mercaptide (N3S) donor ..
  98. pmc The effect of oxidant and the non-oxidant alteration of cellular thiol concentration on the formation of protein mixed-disulfides in HEK 293 cells
    Jasen Lee Gilge
    Department of Chemistry, John Carroll University, University Height, Ohio, United States of America
    PLoS ONE 3:e4015. 2008
    ..proteins were demonstrated in human embryonic kidney 293 cells treated with two different oxidative reagents: diamide and hydrogen peroxide. Diamide is a chemical oxidizing agent whereas hydrogen peroxide is a physiological oxidant...
  99. ncbi Design of novel bicyclic analogues derived from a potent oxytocin antagonist
    George Flouret
    Department of Physiology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    J Pept Sci 12:412-9. 2006
    ..The [desamido(9)] PA-ethylenediamine monoamide, 6, and the dimer ([desamido(9)]-PA)(2) ethylenediamine diamide, 7, had lower potency in the uterotonic assay than PA...
  100. ncbi Insect ryanodine receptors: molecular targets for novel pest control chemicals
    David B Sattelle
    MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK
    Invert Neurosci 8:107-19. 2008
    ..The phthalic acid diamide class has yielded flubendiamide, the first synthetic ryanodine receptor insecticide to be commercialized...
  101. ncbi Enantiomeric separation using temperature-responsive chiral polymers composed of L-valine diamide derivatives in aqueous liquid chromatography
    Kaori Kurata
    Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachiouji, Tokyo 192 0392, Japan
    J Chromatogr A 1012:47-56. 2003
    ..could not provide enantioselectivity for the amino acid derivatives in aqueous media, although the chiral valine diamide moieties were effective for enantiomer separation in non-aqueous media...

Research Grants25

  1. Mitochondrial Iron-Sulfur cluster mechanisms and therapy in Friedreich's ataxia
    Gino A Cortopassi; Fiscal Year: 2013
    ..In Aim 3 we will test the activity of the 9 most diamide-protective drugs for their ability to rescue frataxin expression, iron-sulfur cluster defects, and auranofin ..
  2. TOXICITY OF CARDIAC HYPOXIA AND REOXYGENATION
    JAMES KEHRER; Fiscal Year: 1991
    ..lysis at reoxygenation will be further studied by assessing the effect of 2 additional inhibitors of this enzyme, diamide and vanadium, on cardiac LDH release...
  3. Biomedical Polymers With Heparin-Binding End Groups
    Robert Ward; Fiscal Year: 2004
    ..A novel diamine-diamide-alcohol (PIME-SME) synthesis procedure will be optimized for high yield and low cost...
  4. MECHANISM OF SELENIUM INDUCED CATARACT
    Thomas Shearer; Fiscal Year: 2002
    ..proteolytic- insolubilization mechanism occurs in most other models of rodent cataracts (galactose, xylose, H2O2, diamide, A23187, BSO), regardless of the initial insult allowing influx of calcium, and even in normal maturation of ..
  5. CHELATE CONJUGATED RADIOLABELED MONOCLONAL ANTIBODIES
    Richard Reba; Fiscal Year: 1990
    ..The diamide dithiol derivatives are labeled with Tc-99m before and after the conjugation to antibody...
  6. REDOX REGULATION OF TRANSCRIPTION FACTOR FUNCTION
    W Moye Rowley; Fiscal Year: 2003
    ..Truncated mutant forms of yAP-1 are hyper-resistant to diamide stress but hypersensitive to H2O2...
  7. Polyurethane Biomaterials with Heparin Binding Sites
    Robert Ward; Fiscal Year: 2002
    ..A diamine-diamide-diol (PIME), which has an established ability to bind heparin, will replace some of the normal diol 'chain ..
  8. OXIDANT REGULATION OF RENAL CELL GROWTH AND VIABILITY
    CHERYL SCHEID; Fiscal Year: 1999
    ..The cells will be treated with hydrogen peroxide (a representative oxidant) or diamide (to deplete sulfhydryl groups) and then examined by northern blotting, western blotting and if necessary RT-PCR ..
  9. REGULATION AND FUNCTION OF LUNG SPECIFIC FREAC GENES
    Ye Shih Ho; Fiscal Year: 2002
    ..abstract_text> ..
  10. Structure/Function of Peroxisomes in Neuroinflammation
    Inderjit Singh; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  11. Imbalancing DNA BER to enhance Ovarian Tumor Sensitivity
    Mark Kelley; Fiscal Year: 2008
    ..abstract_text> ..
  12. SYNTHESIS OF BIOLOGICALLY ACTIVE NATURAL PRODUCTS
    BARRY SNIDER; Fiscal Year: 2009
    ..In the course of these studies new methods will be developed that are of general utility for preparing drugs more economically. ..
  13. Nitric oxide and mitochondrial biogenesis in sepsis
    Claude A Piantadosi; Fiscal Year: 2010
    ..abstract_text> ..
  14. Glutaredoxin, Macrophage Death and Atherosclerosis
    RETO H R ASMIS; Fiscal Year: 2010
    ..The proposed studies will test this hypothesis and examine possible molecular mechanisms of Grx-mediated protection of macrophages from mitochondrial dysfunction and cell death. ..
  15. Antioxidant Interaction of Selenium and Vitamins C & E
    James May; Fiscal Year: 2008
    ..Using this approach, it will be possible to relate the crucial, mitochondrial functions of selenium and vitamins C and E to the antioxidant defenses of the whole animal. ..
  16. Therapeutic/Mechanistic Role of Ape1 in Germ Cell Tumors
    Mark Kelley; Fiscal Year: 2007
    ..If any mutants are shown to sensitize the GCT cell lines to chemo-/IR agents, as expected, this will set the stage for future gene therapy approaches to sensitize GCT's to therapy. ..
  17. COAT-Platelets
    George Dale; Fiscal Year: 2005
    ..abstract_text> ..
  18. MITOCHONDRIAL PROTEIN IMPORT IN AFRICAN TRYPANOSOMES
    Minu Chaudhuri; Fiscal Year: 2002
    ..Understanding of this developmental process will elucidate one of the major aspects of trypanosomal parasitism. This in turn will help us to design rational trypanocidal chemotherapeutic strategies. ..
  19. Antioxidant Functions of Lipoic Acid
    James May; Fiscal Year: 2004
    ..Despite the widespread use of lipoic acid, these studies are necessary to establish the biological basis and rationale for the clinical use of the agent. ..
  20. POSTDOCTORAL HEMATOLOGY RESEARCH TRAINING
    George Dale; Fiscal Year: 2003
    ..Francis Fdt. (OUHSC). All members of the faculty work closely together under Specialized Center of Research (SCOR) and Program Project (PP)...
  21. Antioxidant vitamins in models of Alzheimer's Disease
    James May; Fiscal Year: 2008
    ..If antioxidant vitamins ameliorate toxicity in cell and animal models of Alzheimer's disease, then oxidant stress is involved in disease progression, and antioxidant vitamin supplements may be beneficial. ..
  22. Lung Injury Protection by Coagulation Blockade
    CLAUDE PIANTADOSI; Fiscal Year: 2008
    ..A successfully optimized strategy could greatly attenuate persistence of pulmonary inflammation and facilitate the resolution of human ARDS. ..
  23. Mechanism of Actions of Multitasking of Statins in AD
    Inderjit Singh; Fiscal Year: 2006
    ..abstract_text> ..
  24. OXIDATIVE DNA DAMAGE AND REPAIR IN CNS CELLS
    Mark Kelley; Fiscal Year: 2003
    ..abstract_text> ..
  25. DEVELOPMENT OF MORE EFFICIENT CHIRAL STATIONARY PHASES
    Tingyu Li; Fiscal Year: 2002
    ..The chemistry associated with the characterization of chiral stationary phases on silica gel will also be studied. ..