acrylamide

Summary

Summary: A colorless, odorless, highly water soluble vinyl monomer formed from the hydration of acrylonitrile. It is primarily used in research laboratories for electrophoresis, chromatography, and electron microscopy and in the sewage and wastewater treatment industries.

Top Publications

  1. ncbi Concentrations of free amino acids and sugars in nine potato varieties: effects of storage and relationship with acrylamide formation
    Nigel G Halford
    Plant Biology and Crop Science Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom
    J Agric Food Chem 60:12044-55. 2012
  2. pmc Birth weight, head circumference, and prenatal exposure to acrylamide from maternal diet: the European prospective mother-child study (NewGeneris)
    Marie Pedersen
    Centre for Research in Environmental Epidemiology CREAL, Barcelona, Spain
    Environ Health Perspect 120:1739-45. 2012
  3. ncbi Analysis of acrylamide, a carcinogen formed in heated foodstuffs
    Eden Tareke
    Department of Environmental Chemistry, Stockholm University, S 106 91 Stockholm, Sweden
    J Agric Food Chem 50:4998-5006. 2002
  4. ncbi Neuroprotective effect of crocin on acrylamide-induced cytotoxicity in PC12 cells
    Soghra Mehri
    Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
    Cell Mol Neurobiol 32:227-35. 2012
  5. ncbi Analysis of hemoglobin adducts from acrylamide, glycidamide, and ethylene oxide in paired mother/cord blood samples from Denmark
    Hans von Stedingk
    Department of Materials and Environmental Chemistry, Environmental Chemistry Unit, Arrhenius Laboratory, Stockholm University, SE 106 91 Stockholm, Sweden
    Chem Res Toxicol 24:1957-65. 2011
  6. ncbi Tuber-specific silencing of asparagine synthetase-1 reduces the acrylamide-forming potential of potatoes grown in the field without affecting tuber shape and yield
    Rekha Chawla
    Simplot Plant Sciences, J R Simplot Company, Boise, ID, USA
    Plant Biotechnol J 10:913-24. 2012
  7. ncbi Acrylamide is formed in the Maillard reaction
    Donald S Mottram
    School of Food Biosciences, The University of Reading, Whiteknights, Reading RG6 6AP, UK
    Nature 419:448-9. 2002
  8. ncbi Acrylamide from Maillard reaction products
    Richard H Stadler
    Nestle Research Center, Lausanne, Switzerland
    Nature 419:449-50. 2002
  9. ncbi Acrylamide-responsive genes in the nematode Caenorhabditis elegans
    Koichi Hasegawa
    Institute for Biological Function, Chubu University, Kasugai 487 8501, Japan
    Toxicol Sci 101:215-25. 2008
  10. ncbi Prenatal exposure to polychlorinated biphenyls and dioxins is associated with increased risk of wheeze and infections in infants
    Solvor Berntsen Stølevik
    Norwegian Institute of Public Health, NO 0403 Oslo, Norway
    Food Chem Toxicol 49:1843-8. 2011

Detail Information

Publications311 found, 100 shown here

  1. ncbi Concentrations of free amino acids and sugars in nine potato varieties: effects of storage and relationship with acrylamide formation
    Nigel G Halford
    Plant Biology and Crop Science Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom
    J Agric Food Chem 60:12044-55. 2012
    b>Acrylamide forms during cooking and processing predominately from the reaction of free asparagine and reducing sugars in the Maillard reaction...
  2. pmc Birth weight, head circumference, and prenatal exposure to acrylamide from maternal diet: the European prospective mother-child study (NewGeneris)
    Marie Pedersen
    Centre for Research in Environmental Epidemiology CREAL, Barcelona, Spain
    Environ Health Perspect 120:1739-45. 2012
    b>Acrylamide is a common dietary exposure that crosses the human placenta. It is classified as a probable human carcinogen, and developmental toxicity has been observed in rodents.
  3. ncbi Analysis of acrylamide, a carcinogen formed in heated foodstuffs
    Eden Tareke
    Department of Environmental Chemistry, Stockholm University, S 106 91 Stockholm, Sweden
    J Agric Food Chem 50:4998-5006. 2002
    Reaction products (adducts) of acrylamide with N termini of hemoglobin (Hb) are regularly observed in persons without known exposure...
  4. ncbi Neuroprotective effect of crocin on acrylamide-induced cytotoxicity in PC12 cells
    Soghra Mehri
    Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
    Cell Mol Neurobiol 32:227-35. 2012
    b>Acrylamide (ACR) is a potent neurotoxic in human and animal models. In this study, the effect of crocin, main constituent of Crocus sativus L...
  5. ncbi Analysis of hemoglobin adducts from acrylamide, glycidamide, and ethylene oxide in paired mother/cord blood samples from Denmark
    Hans von Stedingk
    Department of Materials and Environmental Chemistry, Environmental Chemistry Unit, Arrhenius Laboratory, Stockholm University, SE 106 91 Stockholm, Sweden
    Chem Res Toxicol 24:1957-65. 2011
    The knowledge about fetal exposure to acrylamide/glycidamide from the maternal exposure through food is limited...
  6. ncbi Tuber-specific silencing of asparagine synthetase-1 reduces the acrylamide-forming potential of potatoes grown in the field without affecting tuber shape and yield
    Rekha Chawla
    Simplot Plant Sciences, J R Simplot Company, Boise, ID, USA
    Plant Biotechnol J 10:913-24. 2012
    ..of asparagine synthetase (Ast)-1 and -2 limits asparagine (ASN) formation and, consequently, reduces the acrylamide-forming potential of tubers...
  7. ncbi Acrylamide is formed in the Maillard reaction
    Donald S Mottram
    School of Food Biosciences, The University of Reading, Whiteknights, Reading RG6 6AP, UK
    Nature 419:448-9. 2002
    Reports of the presence of acrylamide in a range of fried and oven-cooked foods have caused worldwide concern because this compound has been classified as probably carcinogenic in humans...
  8. ncbi Acrylamide from Maillard reaction products
    Richard H Stadler
    Nestle Research Center, Lausanne, Switzerland
    Nature 419:449-50. 2002
    The discovery of the adventitious formation of the potential cancer-causing agent acrylamide in a variety of foods during cooking has raised much concern, but the chemical mechanism(s) governing its production are unclear...
  9. ncbi Acrylamide-responsive genes in the nematode Caenorhabditis elegans
    Koichi Hasegawa
    Institute for Biological Function, Chubu University, Kasugai 487 8501, Japan
    Toxicol Sci 101:215-25. 2008
    As acrylamide is a known neurotoxin for many animals and potential carcinogen for humans, it came as a surprise when the Swedish National Food Agency and Stockholm University reported in 2002 that it is formed during the frying or baking ..
  10. ncbi Prenatal exposure to polychlorinated biphenyls and dioxins is associated with increased risk of wheeze and infections in infants
    Solvor Berntsen Stølevik
    Norwegian Institute of Public Health, NO 0403 Oslo, Norway
    Food Chem Toxicol 49:1843-8. 2011
    ..We here report on the environmental pollutants polychlorinated biphenyls (PCBs) and dioxins, as well as acrylamide generated in food during heat treatment...
  11. ncbi The acrylamide problem: a plant and agronomic science issue
    Nigel G Halford
    Department of Plant Science, Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK
    J Exp Bot 63:2841-51. 2012
    b>Acrylamide, a chemical that is probably carcinogenic in humans and has neurological and reproductive effects, forms from free asparagine and reducing sugars during high-temperature cooking and processing of common foods...
  12. ncbi Acrylamide in French fries: influence of free amino acids and sugars
    Adam Becalski
    Food Research Division, Address Locator 2203D, Health Products and Food Branch, Health Canada, Ottawa, Ontario, Canada K1A 0L2
    J Agric Food Chem 52:3801-6. 2004
    ..5 min using a restaurant fryer. The final product was blast frozen and analyzed for acrylamide. Acrylamide was detected in all samples, but its concentration varied significantly from 50 to 1800 ng/g...
  13. ncbi DNA damage and DNA adduct formation in rat tissues following oral administration of acrylamide
    Isabelle Manière
    Agence Francaise de Securite Sanitaire des Aliments, Laboratoire d Etudes et de Recherches sur les Médicaments Vétérinaires et les Désinfectants, BP 90203, 35302 Fougeres Cedex, France
    Mutat Res 580:119-29. 2005
    b>Acrylamide is present as a contaminant in the human diet in heated food products. It has been found to be carcinogenic in laboratory rats and has been classified as probably carcinogenic in humans...
  14. ncbi Kinetic model for the formation of acrylamide during the finish-frying of commercial french fries
    Jane K Parker
    Department of Food and Nutritional Sciences, University of Reading, Whiteknights, Reading RG6 6AP, United Kingdom
    J Agric Food Chem 60:9321-31. 2012
    b>Acrylamide is formed from reducing sugars and asparagine during the preparation of French fries...
  15. ncbi Acrylamide formation mechanism in heated foods
    David V Zyzak
    The Procter and Gamble Company, Winton Hill Business Center, 6210 Center Hill Avenue, Cincinnati, Ohio 45224, USA
    J Agric Food Chem 51:4782-7. 2003
    Recent findings of a potential human carcinogen, acrylamide, in foods have focused research on the possible mechanisms of formation...
  16. ncbi Effects of genotype and environment on free amino acid levels in wheat grain: implications for acrylamide formation during processing
    Tanya Y Curtis
    Plant Science Department, Rothamsted Research, Harpenden, Hertfordshire, United Kingdom
    J Agric Food Chem 57:1013-21. 2009
    b>Acrylamide forms from free asparagine and reducing sugars during cooking, with asparagine concentration being the key parameter determining the formation in foods produced from wheat flour...
  17. ncbi Influence of fertilization on acrylamide formation during frying of potatoes harvested in 2003
    Tineke De Wilde
    Laboratory of Food Chemistry and Human Nutrition, Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B 9000 Ghent, Belgium
    J Agric Food Chem 54:404-8. 2006
    ..Consequently these factors may influence the acrylamide formation during the preparation of French fries...
  18. pmc The presence of a single N-terminal histidine residue enhances the fusogenic properties of a Membranotropic peptide derived from herpes simplex virus type 1 glycoprotein H
    Stefania Galdiero
    Department of Experimental Medicine, II University of Naples, Via De Crecchio 7, Napoli 80138, Italy
    J Biol Chem 285:17123-36. 2010
    ..Nuclear magnetic resonance (NMR) studies have shown that the addition of the N-terminal His contributes to the formation and stabilization of an alpha-helical domain with high fusion propensity...
  19. ncbi Chemistry, biochemistry, and safety of acrylamide. A review
    Mendel Friedman
    Western Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 800 Buchanan Street, Albany, California 94710, USA
    J Agric Food Chem 51:4504-26. 2003
    b>Acrylamide (CH2=CH-CONH2), an industrially produced alpha,beta-unsaturated (conjugated) reactive molecule, is used worldwide to synthesize polyacrylamide...
  20. pmc Molecular mechanism of glyceraldehyde-3-phosphate dehydrogenase inactivation by α,β-unsaturated carbonyl derivatives
    Christopher J Martyniuk
    Center for Environmental and Human Toxicology, University of Florida, Gainesville, Florida 32601, USA
    Chem Res Toxicol 24:2302-11. 2011
    ..To address these data gaps, we incubated purified human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) with acrylamide (ACR), acrolein, or methylvinyl ketone (MVK)...
  21. ncbi Protective effect of acorus calamus against acrylamide induced neurotoxicity
    Pradeep K Shukla
    Industrial Toxicology Research Centre, Lucknow, India
    Phytother Res 16:256-60. 2002
    Exposure of rats to acrylamide (ACR) caused hind limb paralysis in 58% of the animals on day 10 and decreased behavioural parameters, namely distance travelled, ambulatory time, stereotypic time and basal stereotypic movements compared ..
  22. pmc Diet-induced obesity in male mice is associated with reduced fertility and potentiation of acrylamide-induced reproductive toxicity
    Burhan I Ghanayem
    Laboratory of Pharmacology, Biostatistics Branch, and Cellular and Molecular Pathology Branch, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Biol Reprod 82:96-104. 2010
    ..2, 1.6, and 7.2-fold, respectively. The fertility of male mice was compared through mating with control females. Acrylamide (AA)-induced reproductive toxicity was assessed in obese or lean males treated with water or 25 mg AA kg(-1) day(..
  23. ncbi Rugged LC-MS/MS survey analysis for acrylamide in foods
    John A G Roach
    Center for Food Safety and Applied Nutrition, US Food and Drug Administration, 5100 Paint Branch Parkway, College Park, Maryland 20740, USA
    J Agric Food Chem 51:7547-54. 2003
    The described liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the detection of acrylamide in food entails aqueous room temperature extraction, SPE cleanup, and analysis by LC-MS/MS...
  24. ncbi Dietary acrylamide intake is not associated with gastrointestinal cancer risk
    Janneke G F Hogervorst
    Department of Epidemiology, GROW School for Oncology and Developmental Biology, Maastricht University, Maastricht 6200 MD, The Netherlands
    J Nutr 138:2229-36. 2008
    b>Acrylamide is a probable human carcinogen that was detected in several heat-treated foods, such as French fries and crisps, in 2002. Prospective studies are needed on acrylamide and human cancer risk...
  25. ncbi Toxicity of acrylamide and evaluation of its exposure in baby foods
    Pinar Erkekoglu
    Department of Toxicology, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey
    Nutr Res Rev 23:323-33. 2010
    ..b>Acrylamide, an α,β-unsaturated (conjugated) reactive molecule, can be detected as a contaminant in several foodstuffs ..
  26. ncbi Dietary acrylamide intake and risk of colorectal cancer in a prospective cohort of men
    Susanna C Larsson
    Division of Nutritional Epidemiology, The National Institute of Environmental Medicine, Karolinska Institutet, PO Box 210, SE 17177 Stockholm, Sweden
    Eur J Cancer 45:513-6. 2009
    b>Acrylamide is a probable human carcinogen that causes cancer at multiple sites in animal models. However, whether dietary acrylamide intake increases the risk of colorectal cancer in humans is unclear...
  27. pmc A novel system to accelerate the progression of nerve degeneration in transgenic mouse models of neuropathies
    Osefame Ewaleifoh
    Department of Neurology, Johns Hopkins University, Baltimore, MD USA
    Exp Neurol 237:153-9. 2012
    ..Here, we present a novel technique using acrylamide, an axonal neurotoxin, to provoke rapid axonal degeneration in murine models of neuropathies...
  28. ncbi Acrylamide: review of toxicity data and dose-response analyses for cancer and noncancer effects
    A Shipp
    ENVIRON International Corporation, 602 East Georgia Street, Ruston, LA 07290, USA
    Crit Rev Toxicol 36:481-608. 2006
    b>Acrylamide (ACR) is used in the manufacture of polyacrylamides and has recently been shown to form when foods, typically containing certain nutrients, are cooked at normal cooking temperatures (e.g., frying, grilling or baking)...
  29. ncbi Influence of sulfur fertilization on the amounts of free amino acids in wheat. correlation with baking properties as well as with 3-aminopropionamide and acrylamide generation during baking
    Michael Granvogl
    Chair for Food Chemistry, Technical University of Munich, Lichtenbergstrasse 4, D 85748 Garching, Germany
    J Agric Food Chem 55:4271-7. 2007
    ..the high amount of Asn in flours 1 and 2, heating of both flours led to the generation of very high amounts of acrylamide (1.7-3.1 mg/kg) as well as of 3-aminopropionamide (40-76 mg/kg)...
  30. ncbi Pre-diagnostic acrylamide exposure and survival after breast cancer among postmenopausal Danish women
    Anja Olsen
    Danish Cancer Society Research Center, Danish Cancer Society, Copenhagen, Denmark
    Toxicology 296:67-72. 2012
    b>Acrylamide is a probable human carcinogen, with industrial contact, tobacco smoking and foods processed at high temperatures as the main routes of exposure...
  31. ncbi Urinary mercapturic acids and a hemoglobin adduct for the dosimetry of acrylamide exposure in smokers and nonsmokers
    Michael Urban
    ABF Analytisch biologisches Forschungslabor GmbH, Munchen, Germany
    Inhal Toxicol 18:831-9. 2006
    b>Acrylamide, used in the manufacture of polyacrylamide and grouting agents, is also present in the diet and tobacco smoke. It is a neurotoxin and a probable human carcinogen...
  32. ncbi Fast axonal transport: a site of acrylamide neurotoxicity?
    Dale W Sickles
    Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta 30912 2000, USA
    Neurotoxicology 23:223-51. 2002
    The cellular and molecular site and mode of action of acrylamide (ACR) leading to neurotoxicity has been investigated for four decades, without resolution...
  33. pmc Analysis of a membrane interacting region of herpes simplex virus type 1 glycoprotein H
    Stefania Galdiero
    Department of Biological Sciences, Division of Biostructures, University of Naples Federico II, Via Mezzocannone 16, 80134, Napoli, Italy
    J Biol Chem 283:29993-30009. 2008
    ....
  34. ncbi Investigations of factors that influence the acrylamide content of heated foodstuffs
    Per Rydberg
    Department of Environmental Chemistry, Stockholm University, S 106 91 Stockholm, Sweden
    J Agric Food Chem 51:7012-8. 2003
    The acrylamide content of heated foodstuffs should be considered to be the net result of complex reactions leading to the formation and elimination/degradation of this compound...
  35. ncbi Effects of fried potato chip supplementation on mouse pregnancy and fetal development
    Hassan I El-Sayyad
    Department of Zoology, Faculty of Science, Mansoura University, Mansoura, Egypt
    Nutrition 27:343-50. 2011
    b>Acrylamide (ACR), a proven rodent carcinogen, is present at significantly high quantities in commonly consumed foods such as potato chips, raising a health concern worldwide.
  36. ncbi Dietary acrylamide exposure and hemoglobin adducts--National Health and Nutrition Examination Survey (2003-04)
    N L Tran
    E x ponent, Inc, Washington, DC 20036, USA
    Food Chem Toxicol 48:3098-108. 2010
    ..Measured acrylamide (AA-Hb) and glycidamide (Gly-Hb) hemoglobin adducts for over 7000 participants >3 years, 24-h dietary recall, ..
  37. ncbi Exploring tryptophan dynamics in acid-induced molten globule state of bovine alpha-lactalbumin: a wavelength-selective fluorescence approach
    Devaki A Kelkar
    Centre for Cellular and Molecular Biology, Council of Scientific and Industrial Research, Uppal Road, Hyderabad, India
    Eur Biophys J 39:1453-63. 2010
    ..Quenching of BLA tryptophan fluorescence using quenchers of varying polarity (acrylamide and trichloroethanol) reveals varying degrees of accessibility of tryptophan residues, characteristic of native ..
  38. ncbi Acrylamide axonopathy revisited
    R M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, 111 E 210th St, Bronx, NY 10467, USA
    Toxicol Appl Pharmacol 188:135-53. 2003
    ..of axons in the CNS and PNS have been considered to be the characteristic neuropathological features of acrylamide (ACR) neuropathy...
  39. ncbi NTP-CERHR expert panel report on the reproductive and developmental toxicity of acrylamide
    Jeanne Manson
    University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Birth Defects Res B Dev Reprod Toxicol 74:17-113. 2005
  40. ncbi A review of mechanisms of acrylamide carcinogenicity
    Ahmad Besaratinia
    Division of Biology, Beckman Research Institute of the City of Hope National Medical Center, 1450 East Duarte Road, Duarte, CA 91010, USA
    Carcinogenesis 28:519-28. 2007
    The fact that acrylamide, a proven rodent carcinogen, is present in significant quantities (up to several mg/kg of foodstuff) in a wide range of commonly consumed human foods is alarming...
  41. ncbi Trans-placental exposure of neonates to acrylamide--a pilot study
    T Schettgen
    Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, University of Erlangen Nuremberg, Schillerstrasse 25 29, 91054 Erlangen, Germany
    Int Arch Occup Environ Health 77:213-6. 2004
    b>Acrylamide (AA) (CAS No 79-06-1) has most recently been identified as a food-borne toxicant generated during the heating process of starch-containing foods...
  42. ncbi Nerve terminals as the primary site of acrylamide action: a hypothesis
    R M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, NY 10467, USA
    Neurotoxicology 23:43-59. 2002
    b>Acrylamide (ACR) is considered to be prototypical among chemicals that cause a central-peripheral distal axonopathy...
  43. ncbi Acrylamide neuropathy. II. Spatiotemporal characteristics of nerve cell damage in brainstem and spinal cord
    E J Lehning
    Department of Anesthesiology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, NY 10467, USA
    Neurotoxicology 23:415-29. 2002
    Previous studies of acrylamide (ACR) neuropathy in rat PNS [Toxicol. Appl. Pharmacol...
  44. ncbi Evidence-based dose-response assessment for thyroid tumorigenesis from acrylamide
    Michael Dourson
    Toxicology Excellence for Risk Assessment TERA N A, 2300 Montana Ave Suite 409, Cincinnati, OH 45211, USA
    Regul Toxicol Pharmacol 52:264-89. 2008
    b>Acrylamide is commonly found in various foods. Cancer studies in rats have reported increases in tumors of the thyroid, mammary tissues, tunica vaginalis of the testis, and sometimes tumors at other sites...
  45. ncbi Genotoxicity of acrylamide in human hepatoma G2 (HepG2) cells
    Liping Jiang
    China Japanese Joint Institute for Medical and Pharmaceutical Science, Dalian Medical University, 465 Zhongshan Road, Dalian 116027, Liaoning, China
    Toxicol In Vitro 21:1486-92. 2007
    The recent finding that acrylamide (AA), a carcinogen in animal experiments and a probable human carcinogen, is formed in foods during cooking raises human health concerns...
  46. pmc The acrylamide (S)-2 as a positive and negative modulator of Kv7 channels expressed in Xenopus laevis oocytes
    Sigrid Marie Blom
    Section of Early Target Pharmacology and Physiology, H Lundbeck A S, Copenhagen, Denmark
    PLoS ONE 4:e8251. 2009
    ....
  47. ncbi Soxhlet extraction of acrylamide from potato chips
    Jörgen R Pedersen
    Arealia AB, Göteborgsv 91B, SE 43137 Mölndal, Sweden
    Analyst 128:332-4. 2003
    The problem of complete extraction of acrylamide from potato chips was investigated. A method was developed based on the Soxhlet extraction technique...
  48. ncbi Formation of high levels of acrylamide during the processing of flour derived from sulfate-deprived wheat
    Nira Muttucumaru
    Crop Performance and Improvement Division, Rothamsted Research, Harpenden, Hertfordshire, United Kingdom
    J Agric Food Chem 54:8951-5. 2006
    ..In baked cereal products, asparagine is the precursor of the suspect carcinogen acrylamide, and when flours from the sulfate-deprived wheat were heated at 160 degrees C for 20 min, levels of acrylamide ..
  49. pmc Axonal protective effects of the myelin-associated glycoprotein
    Thien Nguyen
    Department of Neurology, The Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
    J Neurosci 29:630-7. 2009
    ..Exploiting this pathway may lead to therapeutic strategies for neurological diseases characterized by axonal loss...
  50. ncbi Acrylamide and cancer risk--expert risk assessments and the public debate
    C Rudén
    Philosophy Unit, Royal Institute of Technology, Teknikringen 78B, S 100 44 Stockholm, Sweden
    Food Chem Toxicol 42:335-49. 2004
    This study has two parts. In the first part, fourteen carcinogen risk assessments of acrylamide made by different expert groups during the years 1976-2002 are compared in terms of their overall conclusions and their use of primary data...
  51. ncbi Hemoglobin adducts of ethylene oxide, propylene oxide, acrylonitrile and acrylamide-biomarkers in occupational and environmental medicine
    Thomas Schettgen
    Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, Friedrich Alexander University of Erlangen Nurnberg, Schillerstrasse 25 29, D 91054 Erlangen, Germany
    Toxicol Lett 134:65-70. 2002
    ..The levels of the adducts of acrylonitrile (ACN) and acrylamide (AA) were also determined, though they were mainly accounted for by smoking and diet...
  52. ncbi More stable structure of wheat germ lipase at low pH than its native state
    Ejaz Ahmad
    Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India
    Biochimie 92:885-93. 2010
    ..Although enzymatically inactive, acid-induced state at pH 0.8 was found to be structurally more stable than native lipase, as shown by chemical and thermal denaturation profiles...
  53. ncbi Chronic intake of potato chips in humans increases the production of reactive oxygen radicals by leukocytes and increases plasma C-reactive protein: a pilot study
    Marek Naruszewicz
    National Institute of Food and Nutrition, Warsaw, Poland
    Am J Clin Nutr 89:773-7. 2009
    Relatively high concentrations of acrylamide in commonly ingested food products, such as French fries, potato chips, or cereals, may constitute a potential risk to human health.
  54. ncbi The acrylamide intake via some common baby food for children in Sweden during their first year of life--an improved method for analysis of acrylamide
    P Fohgelberg
    National Food Administration, Toxicology Division, Box 622, 75126 Uppsala, Sweden
    Food Chem Toxicol 43:951-9. 2005
    The acrylamide levels in breast milk and the main categories of Swedish baby food products, i.e. breast milk substitute (infant formula), gruel, porridge and canned baby food, have been analysed...
  55. ncbi Inhibition of acrylamide toxicity in mice by three dietary constituents
    Qunying Xie
    Department of Chemical Biology, College of Chemistry and Molecular Engineering, State Key Laboratory of Nuclear Physics and Technology, Peking University, Beijing, People s Republic of China
    J Agric Food Chem 56:6054-60. 2008
    The inhibitory effects of three dietary constituents, tea polyphenols, resveratrol, and diallyl trisulfide, on acrylamide-biomacromolecule (liver DNA, protamine, and hemoglobin) adduct formation at human exposure level were studied by ..
  56. ncbi Acrylamide neurotoxicity: neurological, morhological and molecular endpoints in animal models
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, 111 E 210th St, Bronx, NY 10467, USA
    Adv Exp Med Biol 561:21-37. 2005
    b>Acrylamide (AA) monomer is used in numerous chemical industries and is a contaminant in potato- and grain-based foods prepared at high temperatures...
  57. ncbi The carcinogenicity of acrylamide
    Jerry M Rice
    Department of Oncology, Georgetown University Medical Center, Lombardi Comprehensive Cancer Center, PO Box 571465, 3800 Reservoir Road, NW Washington, DC 20057 1465, USA
    Mutat Res 580:3-20. 2005
    b>Acrylamide is carcinogenic to experimental mice and rats, causing tumors at multiple organ sites in both species when given in drinking water or by other means...
  58. ncbi Toxicokinetics of acrylamide and glycidamide in Fischer 344 rats
    Daniel R Doerge
    National Center for Toxicological Research, Jefferson, AR 72079, USA
    Toxicol Appl Pharmacol 208:199-209. 2005
    b>Acrylamide (AA) is a widely studied industrial chemical that is neurotoxic, mutagenic to somatic and germ cells, and carcinogenic in rodents...
  59. ncbi Enhancement by acrylamide of N-methyl-N-nitrosourea-induced rat mammary tumor development-possible application for a model to detect co-modifiers of carcinogenesis
    Toshio Imai
    Division of Pathology, National Institute of Health Sciences, 1 18 1 Kamiyoga, Setagaya Ku, Tokyo 158 8501, Japan
    Cancer Lett 230:25-32. 2005
    b>Acrylamide (AA) has recently been reported to be spontaneously formed in fried and baked foods with various concentrations...
  60. ncbi Determination of acrylamide level in commercial baby foods and an assessment of infant dietary exposure
    Hanna Mojska
    National Food and Nutrition Institute, 02 903 Warsaw, Poland
    Food Chem Toxicol 50:2722-8. 2012
    b>Acrylamide is a monomer that can form in heated starchy food as a result of Maillard reaction. The adverse effects of acrylamide in humans are neurotoxicity and carcinogenicity...
  61. ncbi Acrylamide-induced changes in the neurofilament protein of rat cerebrum fractions
    Sufang Yu
    Institute of Toxicology, Shandong University, 250012, Jinan, P R China
    Neurochem Res 30:1079-85. 2005
    b>Acrylamide (ACR) is known to produce central-peripheral distal axonopathy, which is characterized by distal swellings and secondary degeneration both in experimental animals and human...
  62. ncbi Dietary exposure to acrylamide in adolescents from a Canadian urban center
    Louise Normandin
    Institut National de Sante Publique du Quebec, Quebec, Quebec, Canada
    Food Chem Toxicol 57:75-83. 2013
    The distribution of acrylamide in food items frequently consumed by Canadian adolescents was determined along with estimates of their contribution to the overall dietary intake of acrylamide...
  63. ncbi Evidence of acrylamide induced oxidative stress and neurotoxicity in Drosophila melanogaster - its amelioration with spice active enrichment: relevance to neuropathy
    Sathya N Prasad
    Department of Biochemistry and Nutrition, CSIR Central Food Technological Research Institute CFTRI, Mysore 570020, Karnataka, India
    Neurotoxicology 33:1254-64. 2012
    b>Acrylamide (ACR) intoxication in its monomeric form leads to neuronal damage in both experimental animals and humans. Oxidative stress is one of the principle mechanisms related to the neurotoxicity of ACR exposure...
  64. ncbi Thermally generated 3-aminopropionamide as a transient intermediate in the formation of acrylamide
    Michael Granvogl
    Chair for Food Chemistry, Technical University of Munich, Garching, Germany
    J Agric Food Chem 54:5933-8. 2006
    ..The results of model studies revealed that, besides acrylamide, 3-aminopropionamide was also formed in amounts of 0.1-0...
  65. ncbi Toxicokinetics of acrylamide in humans after ingestion of a defined dose in a test meal to improve risk assessment for acrylamide carcinogenicity
    Uwe Fuhr
    Department of Pharmacology, University Hospital, University of Cologne, Gleueler Strasse 24, 50931 Cologne, Germany
    Cancer Epidemiol Biomarkers Prev 15:266-71. 2006
    High amounts of acrylamide in some foods result in an estimated daily mean intake of 50 microg for a western style diet. Animal studies have shown the carcinogenicity of acrylamide upon oral exposure...
  66. ncbi Tumorigenicity of acrylamide and its metabolite glycidamide in the neonatal mouse bioassay
    Linda S Von Tungeln
    Division of Biochemical Toxicology, National Center for Toxicological Research, Jefferson, AR 72079, USA
    Int J Cancer 131:2008-15. 2012
    b>Acrylamide is a high-volume industrial chemical, a component of cigarette smoke, and a product formed in certain foods prepared at high temperatures...
  67. ncbi Dietary acrylamide does not increase colon aberrant crypt foci formation in male F344 rats
    Jayadev Raju
    Toxicology Research Division, Bureau of Chemical Safety, Food Directorate, Health Products and Food Branch Health Canada, Ottawa, Ontario, Canada
    Food Chem Toxicol 49:1373-80. 2011
    b>Acrylamide, a known rodent and a probable human carcinogen, is spontaneously formed in foods cooked at high temperature...
  68. ncbi Transmembrane transports of acrylamide and bisphenol A and effects on development of zebrafish (Danio rerio)
    Xun Chang Fei
    State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China
    J Hazard Mater 184:81-8. 2010
    b>Acrylamide (AA) and bisphenol A (BPA) are two kinds of pollutants with different structures and polarities...
  69. ncbi Influence of thermal processing conditions on acrylamide generation and browning in a potato model system
    Thomas M Amrein
    Institute of Food Science and Nutrition, ETH Zurich, Switzerland
    J Agric Food Chem 54:5910-6. 2006
    Fried potato products such as French fries and chips may contain substantial amounts of acrylamide. Numerous efforts are undertaken to minimize the acrylamide content of these products while sensory properties such as color and flavor ..
  70. ncbi Exposure to acrylamide and human cancer--a review and meta-analysis of epidemiologic studies
    C Pelucchi
    Department of Epidemiology, Mario Negri Institute for Pharmacological Research, Milan, Italy
    Ann Oncol 22:1487-99. 2011
    b>Acrylamide has been associated to cancer risk in rodents, but data on humans are inconclusive. We thus carried out a critical review and meta-analysis of studies of exposure to acrylamide and cancer.
  71. ncbi Positive association between urinary levels of 8-hydroxydeoxyguanosine and the acrylamide metabolite N-acetyl-S-(propionamide)-cysteine in adolescents and young adults
    Chien Yu Lin
    Department of Internal Medicine, En Chu Kong Hospital, New Taipei City 237, Taiwan School of Medicine, Fu Jen Catholic University, New Taipei City 242, Taiwan
    J Hazard Mater 261:372-7. 2013
    b>Acrylamide is present in mainstream cigarette smoke and in some foods prepared at high temperatures. Animal studies have shown that acrylamide exposure increases oxidative stress; however, it is not known if this also occurs in humans...
  72. ncbi Procyanidin B2 and a cocoa polyphenolic extract inhibit acrylamide-induced apoptosis in human Caco-2 cells by preventing oxidative stress and activation of JNK pathway
    Ildefonso Rodríguez-Ramiro
    Department of Metabolism and Nutrition, Instituto del Frio ICTAN, Consejo Superior de Investigaciones Cientificas, Jose Antonio Novais 10, Ciudad Universitaria, 28040 Madrid, Spain
    J Nutr Biochem 22:1186-94. 2011
    Humans are exposed to dietary acrylamide (AA) during their lifetime; it is therefore necessary to investigate the mechanisms associated with AA induced toxic effects...
  73. ncbi Association of CYP2E1, GST and mEH genetic polymorphisms with urinary acrylamide metabolites in workers exposed to acrylamide
    Yu fang Huang
    Institute of Environmental and Occupational Health Sciences, National Yang Ming University, Taipei, Taiwan
    Toxicol Lett 203:118-26. 2011
    This study elucidates the association of acrylamide metabolites, N-acetyl-S-(2-carbamoylethyl)-cysteine (AAMA), N-acetyl-S-(1-carbamoyl-2-hydroxyethyl)-cysteine (GAMA2), and N-acetyl-S-(2-carbamoyl-2-hydroxyethyl)-cysteine (GAMA3) in ..
  74. ncbi Effects on the peripheral nervous system of tunnel workers exposed to acrylamide and N-methylolacrylamide
    Helge Kjuus
    Department of Occupational Medicine, National Institute of Occupational Health, Oslo, Norway
    Scand J Work Environ Health 30:21-9. 2004
    This study evaluates the possible toxic effects on the peripheral nervous system of tunnel workers exposed to acrylamide and N-methylolacrylamide during grouting work.
  75. ncbi Potent neuroprotective role of novel melatonin derivatives for management of central neuropathy induced by acrylamide in rats
    Hanaa H Ahmed
    Hormones Department, National Research Centre, Dokki, Cairo, Egypt
    Eur J Med Chem 45:5452-9. 2010
    b>Acrylamide (ACR) has been shown to be a neurotoxic agent for both laboratory animals and human...
  76. ncbi Why asparagine needs carbohydrates to generate acrylamide
    Varoujan A Yaylayan
    Department of Food Science and Agricultural Chemistry, McGill University, 21, 111 Lakeshore, Ste Anne de Bellevue, Quebec, Canada H9X 3V9
    J Agric Food Chem 51:1753-7. 2003
    Structural considerations dictate that asparagine alone may be converted thermally into acrylamide through decarboxylation and deamination reactions...
  77. ncbi The carcinogenicity of dietary acrylamide intake: a comparative discussion of epidemiological and experimental animal research
    Janneke G F Hogervorst
    Department of Epidemiology, GROW School for Oncology and Developmental Biology, Maastricht University, Maastricht, The Netherlands
    Crit Rev Toxicol 40:485-512. 2010
    Since 2002, it is known that the probable human carcinogen acrylamide is present in commonly consumed carbohydrate-rich foods, such as French fries and potato chips...
  78. ncbi In-depth mechanistic study on the formation of acrylamide and other vinylogous compounds by the maillard reaction
    Richard H Stadler
    Nestle Research Center, Vers chez les Blanc, 1000 Lausanne 26, Switzerland
    J Agric Food Chem 52:5550-8. 2004
    The formation of acrylamide was studied in low-moisture Maillard model systems (180 degrees C, 5 min) based on asparagine, reducing sugars, Maillard intermediates, and sugar degradation products...
  79. ncbi Extremely low dose of acrylamide decreases lifespan in Caenorhabditis elegans
    Koichi Hasegawa
    Institute for Biological Function, Chubu University, 1200 Matsumoto cho, Kasugai 487 8501, Japan
    Toxicol Lett 152:183-9. 2004
    The neurotoxic, genotoxic and carcinogenic effects of industrial exposure to acrylamide have been studied in animals and humans for more than 30 years...
  80. ncbi Determination of acrylamide in Chinese traditional carbohydrate-rich foods using gas chromatography with micro-electron capture detector and isotope dilution liquid chromatography combined with electrospray ionization tandem mass spectrometry
    Yu Zhang
    Department of Food Science and Nutrition, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, Zhejiang Province, PR China
    Anal Chim Acta 584:322-32. 2007
    The present study developed two analytical methods for quantification of acrylamide in complex food matrixes, such as Chinese traditional carbohydrate-rich foods...
  81. ncbi Expression analysis of hepatic mitochondria-related genes in mice exposed to acrylamide and glycidamide
    Taewon Lee
    Department of Information and Mathematics, Korea University, Jochiwon, Chungnam, Republic of Korea
    J Toxicol Environ Health A 75:324-39. 2012
    b>Acrylamide (AA) is an industrial chemical that has been extensively investigated for central nervous system (CNS), reproductive, and genetic toxicity...
  82. pmc Molecular mechanism of acrylamide neurotoxicity: lessons learned from organic chemistry
    Richard M LoPachin
    Department of Anesthesiology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, New York 10467, USA
    Environ Health Perspect 120:1650-7. 2012
    b>Acrylamide (ACR) produces cumulative neurotoxicity in exposed humans and laboratory animals through a direct inhibitory effect on presynaptic function.
  83. ncbi Acrylamide formation in low-fat potato snacks and its correlation with colour development
    Małgorzata A Majcher
    Department of Food Science and Nutrition, August Cieszkowski Agricultural University of Poznan, Wojska Polskiego 31, 60 624 Poznan, Poland
    Food Addit Contam 24:337-42. 2007
    ..Due to the character of the product, the level of acrylamide was analysed...
  84. pmc Role of the Nrf2-ARE pathway in acrylamide neurotoxicity
    Lihai Zhang
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, NY 10467 2490, United States
    Toxicol Lett 205:1-7. 2011
    b>Acrylamide (ACR) intoxication is associated with selective nerve terminal damage in the central and peripheral nervous systems...
  85. ncbi The fundamental radiation properties of normoxic polymer gel dosimeters: a comparison between a methacrylic acid based gel and acrylamide based gels
    Y De Deene
    Radiotherapy and Nuclear Medicine, Ghent University Hospital, De Pintelaan 185, 9000 Gent, Belgium
    Phys Med Biol 51:653-73. 2006
    ..Other combinations of monomer and gelling agent may result in more reliable normoxic polymer gel dosimeters...
  86. ncbi Effects of consumer food preparation on acrylamide formation
    Lauren S Jackson
    U S Food and Drug Administration, National Center for Food Safety and Technology NCFST, 6502 S Archer Rd, Summit Argo, IL 60501, USA
    Adv Exp Med Biol 561:447-65. 2005
    b>Acrylamide is formed in high-carbohydrate foods during high temperature processes such as frying, baking, roasting and extrusion...
  87. ncbi Acrylamide-induced oxidative stress in human erythrocytes
    Betul Catalgol
    Department of Pharmaceutical Toxicology, Istanbul University, Turkey
    Hum Exp Toxicol 28:611-7. 2009
    b>Acrylamide (AA), a widely used industrial chemical, is shown to be neurotoxic, mutagenic and carcinogenic...
  88. ncbi Ethanol enhanced the genotoxicity of acrylamide in human, metabolically competent HepG2 cells by CYP2E1 induction and glutathione depletion
    Evelyn Lamy
    Institute of Indoor and Environmental Toxicology, University of Giessen, Aulweg 123, 35385 Giessen, Germany
    Int J Hyg Environ Health 211:74-81. 2008
    In the present study, the genotoxicity of acrylamide (AA) was investigated in HepG2 cells using SCGE...
  89. ncbi Toxic effects of acrylamide on survival, development and haemocytes of Musca domestica
    T Szczerbina
    Department of Cytology and Histology, Institute of Zoology, Jagiellonian University, Ingardena 6, 30 060 Krakow, Poland
    Food Chem Toxicol 46:2316-9. 2008
    The influence of acrylamide, a potentially toxic substance present in some types of food, on survival, postembryonic development and haemocytes, insect's blood cells, of the housefly was examined...
  90. ncbi Acrylamide neuropathy. III. Spatiotemporal characteristics of nerve cell damage in forebrain
    E J Lehning
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, 111 E 210th Street, Bronx, NY 10467, USA
    Neurotoxicology 24:125-36. 2003
    Previous studies of acrylamide (ACR) neuropathy in rat PNS [Toxicol. Appl. Pharmacol...
  91. ncbi Acrylamide levels in Finnish foodstuffs analysed with liquid chromatography tandem mass spectrometry
    Susanna Eerola
    Chemistry and Toxicology Unit, Finnish National Food Safety Authority, Helsinki, Finland
    Mol Nutr Food Res 51:239-47. 2007
    ..clean-up and HPLC with tandem mass spectrometric detection (LC-MS/MS) was validated for the routine analysis of acrylamide in various foodstuffs...
  92. ncbi Hemoglobin adducts as a measure of variations in exposure to acrylamide in food and comparison to questionnaire data
    Anna C Vikström
    Department of Materials and Environmental Chemistry, Environmental Chemistry Unit, Stockholm University, SE 104 05 Stockholm, Sweden
    Food Chem Toxicol 50:2531-9. 2012
    Measurement of haemoglobin (Hb) adducts from acrylamide (AA) and its metabolite glycidamide (GA) is a possibility to improve the exposure assessment in epidemiological studies of AA intake from food...
  93. ncbi Effects of acrylamide exposure on serum hormones, gene expression, cell proliferation, and histopathology in male reproductive tissues of Fischer 344 rats
    L Camacho
    Division of Biochemical Toxicology, National Center for Toxicological Research, U S Food and Drug Administration, 3900 NCTR Road, Jefferson, AR 72079, USA
    Toxicol Lett 211:135-43. 2012
    b>Acrylamide (AA) is a reactive monomer used in many technological applications, but it is the incidental formation during cooking of common starchy foods that leads to pervasive human exposure, typically in the range of 1 μg/kg body ..
  94. ncbi Effect of sub-acute exposure to acrylamide on GABAergic neurons and astrocytes in weaning rat cerebellum
    Jing Shi
    Department of Anatomy, Histology and Embryology, Basic Medical College, Guangdong Pharmaceutical University, Guangzhou, China
    Toxicol Ind Health 28:10-20. 2012
    Occupational exposure and experimental intoxication of acrylamide (ACR) can produce skeletal muscle weakness and ataxia...
  95. pmc Dietary acrylamide intake and the risk of cancer among Finnish male smokers
    T Hirvonen
    Finnish Food Safety Authority, Risk Assessment Unit, Mustialankatu 3, FI 00790 Helsinki, Finland
    Cancer Causes Control 21:2223-9. 2010
    To assess the association between dietary acrylamide intake and the risk of cancer among male smokers.
  96. ncbi Acrylamide-induced oxidative stress and biochemical perturbations in rats
    M I Yousef
    Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt
    Toxicology 219:133-41. 2006
    b>Acrylamide is neurotoxic to experimental animals and humans. Also, it has mutagenic and carcinogenic effects...
  97. ncbi Assessment of the astrogliotic responses of three human astrocytoma cell lines to ethanol, trimethyltin chloride and acrylamide
    Lindsay J Holden
    School of Life and Health Sciences, Aston University, Birmingham B4 7ET, UK
    Toxicology 241:75-83. 2007
    ..and U373-MG human astrocytoma lines were determined after exposure to ethanol, trimethyltin chloride (TMTC), and acrylamide over 4, 16, and 24h...
  98. ncbi Review of methods for the reduction of dietary content and toxicity of acrylamide
    Mendel Friedman
    Western Regional Research Center, Agricultural Research Service, U S Department of Agriculture, Albany, California 94710, USA
    J Agric Food Chem 56:6113-40. 2008
    Potentially toxic acrylamide is largely derived from heat-induced reactions between the amino group of the free amino acid asparagine and carbonyl groups of glucose and fructose in cereals, potatoes, and other plant-derived foods...
  99. ncbi Cytoplasmic desmosomes and intermediate filament disturbance following acrylamide treatment in cultured rat keratinocytes
    A H Shabana
    Laboratoire de Biologie Odontologie, Institut Biomedical des Cordeliers, France
    Tissue Cell 26:43-55. 1994
    ..of tonofilaments and desmosomes of rat lingual and epidermal keratinocytes after treatment of the cells with acrylamide in culture...
  100. pmc A prospective study on dietary acrylamide intake and the risk for breast, endometrial, and ovarian cancers
    Kathryn M Wilson
    Department of Epidemiology, Harvard School of Public Health, Boston, Massachusetts, USA
    Cancer Epidemiol Biomarkers Prev 19:2503-15. 2010
    b>Acrylamide is a probable human carcinogen formed during cooking of many common foods...
  101. ncbi In vivo doses of acrylamide and glycidamide in humans after intake of acrylamide-rich food
    Anna C Vikström
    Department of Materials and Environmental Chemistry, Environmental Chemistry Unit, Arrhenius Laboratory, Stockholm University, SE 106 91 Stockholm, Sweden
    Toxicol Sci 119:41-9. 2011
    For assessment of cancer risk from acrylamide (AA) exposure through food, the relation between intake from food in humans and the in vivo doses (area under the concentration-time curve, AUC) of AA (AUC-AA) and of its genotoxic metabolite ..

Research Grants62

  1. The Nerve Terminal as the Site of Action for Type-2 Alkenes
    RICHARD MICHAEL LOPACHIN; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Our research (yrs 1-24) has shown that subchronic systemic acrylamide (ACR) exposure causes cumulative neurotoxicity mediated by selective damage of central and peripheral nerve terminals...
  2. Copolymer hydrogel microspheres for analysis of kinase activity in cell lysates
    Sean P Palecek; Fiscal Year: 2010
    ..Then these substrates will be copolymerized with acrylamide and crosslinker in an emulsion polymerization to generate microspheres via two-phase microfluidic flow...
  3. DIETARY AND HORMONAL DETERMINANTS OF CANCER IN WOMEN
    Meir Stampfer; Fiscal Year: 2013
    ..will be assessed, in Project 3, we will evaluate vitamin D, melatonin, dietary factors (flavonoids, lactose and acrylamide) and plasma markers of inflammation in relation to ovarian cancer risk...
  4. Quantitative molecular profiling of tumor biomarkers with multi-color monovalent
    Hongyan Liu; Fiscal Year: 2012
    ..This gel system is highly capable of separating QDs because QDs cannot enter or be fractionated by poly-acrylamide gel...
  5. Hydrolytically stable hydrophilic CE coatings for protein characterization
    JUNIOR EMIRO SANDOVAL; Fiscal Year: 2012
    ..Finally, polymerization of an N-substituted acrylamide monomer (namely, N-acryloyl- aminopropanol), initiated at the benzyl chloride group in the presence of a Cu-..
  6. "Smart" Materials to Engineer a More Complete Stem Cell Niche
    Adam Engler; Fiscal Year: 2009
    ..These gels, comprised of hyaluronic acid-co-acrylamide polymer, should present spatially- and temporally-controlled matrix properties that mimic their presentation ..
  7. Molecular Micellar-Polyacrylamide Gel Electrophoresis (MoMi-PAGE)
    Isiah M Warner; Fiscal Year: 2010
    ..the use of molecular micelles to replace sodium dodecyl sulfate (SDS) and other conventional micelles in poly- acrylamide gel electrophoresis...
  8. GE TYPHOON 9410 VARIABLE MODE IMAGER
    JOHN RUTLEDGE BERMINGHAM; Fiscal Year: 2010
    ..perform multiplex PCR reactions for genotyping, as well as detection of proteins transferred to membranes from acrylamide gels (western blots) or from tissue sections (histoblots)...
  9. SIGNIFICANCE OF EPOXIDE GENERATED 3-ALKYL-URACIL IN DNA
    JEROME SOLOMON; Fiscal Year: 1999
    ..Acrylonitrile (AN) and acrylamide (AM) are important industrial chemicals with toxic, mutagenic and carcinogenic activity...
  10. DNA-crosslinked gels: New advanced biomaterials
    NOSHIR LANGRANA; Fiscal Year: 2006
    ..gels provide an ideal framework for studying cell interactions with substrates of varying stiffness because the acrylamide chemistry enables easy attachment of the gel to glass...
  11. Innovations to Inform Occupational Disease Etiology Using Biomonitoring Methods
    JO ANNA SHIMEK; Fiscal Year: 2009
    ..Additionally, we hypothesize that hemoglobin adducts of 1, 3-butadiene and acrylamide are valid biomarkers of exposure and can be used to validate the exposure scoring method...
  12. MUTATION IDENTIFICATION IN HERITABLE CHONDRODYSTROPHIES
    Deborah Redford Badwal; Fiscal Year: 1993
    ..Each fragment will be applied to acrylamide and denaturing gradient gel electrophoresis to identify the one containing the mutation...
  13. Targeted DNA extraction for Tuberculosis PCR from sputum
    Eric Houpt; Fiscal Year: 2007
    ..This phase I proposal will test two new targeted Tb DNA extraction methods, a bead-based or acrylamide gel-based capture of lysed Tb DNA, which we hypothesize will increase PCR sensitivity in smear-negative sputa...
  14. ENVIRONMENTAL NEUROTOXICANTS & THE AXONAL CYTOSKELETON
    Anthony DeCaprio; Fiscal Year: 1992
    ..includes n-hexane, 2,5-hexanedione (2,5-HD), carbon disulfide (CS2), beta, beta-iminodipropionitrile (IDPN), an acrylamide. These agents produce cytoskeletal disorganization and NF accumulation within susceptible nerve fibers...
  15. Improved Zwitterions for Proteomics
    LEO RODKEY; Fiscal Year: 2005
    ..Narrow range ampholytes will be synthesized directly by first altering the polyamine molecules by reaction with acrylamide or acrylonitrile followed by reaction with optimal ratios of acrylic acid...
  16. RAPID PRENATAL SEXING OF CLEAVING MOUSE EMBRYOS
    JON GORDON; Fiscal Year: 1992
    ..polymerization in the presence of 32P-dCTP, and denaturation, the PCR reaction mixture will be subjected to acrylamide gel electrophoresis...
  17. Structure-Function Studies of Annexin I and II
    Hartmut Luecke; Fiscal Year: 2006
    ..3) Employ tryptophan fluorescence spectroscopy including acrylamide quenching and steady-state anisotropy measurements to analyze conformational changes as well as membrane ..
  18. Novel Comprehensive Proteome Analyses of Metastasis
    David Speicher; Fiscal Year: 2001
    ..This method, termed microscale solution IEF (mu sol-IEF), uses small chambers separated by large pore acrylamide discs with immobilines at specific pH's to separate cell extracts into well resolved pools based upon pI's...
  19. Novel Comprehensive Proteome Analyses of Metastasis
    David Speicher; Fiscal Year: 2004
    ..This method, termed microscale solution IEF (mu sol-IEF), uses small chambers separated by large pore acrylamide discs with immobilines at specific pH's to separate cell extracts into well resolved pools based upon pI's...
  20. EFFECT OF PAROTID FUNCTION ON SALIVA & CELLS
    DORTHEA JOHNSON; Fiscal Year: 1992
    ..The composition of proteins will be determined by densitometric scanning of color positives taken of acrylamide gels following SDS-PAGE...
  21. TUMOR NECROSIS FACTOR: PURIFICATION AND MODE OF ACTION
    Saul Green; Fiscal Year: 1980
    ..glycoprotein in nature, has a molecular weight of 150,000 and it dissociates into four protein subunits during acrylamide electrophoresis...
  22. SCRUB TYPHUS VARIATION: PROTEINS AND MEMBRANE ANTIGENS
    Barbara Hanson; Fiscal Year: 1990
    ..antibodies specific for identified epitopes and by comparison of protein fragments from different strains by acrylamide gel electrophoresis and immunoblotting; (2) correlation of epitopes with specific amino acid sequences of the ..
  23. MECHANISMS OF DYING BACK NEUROPATHIES
    MOHAMED ABOU DONIA; Fiscal Year: 1993
    The goal of this proposal is to examine the mechanism(s) through which covalent binding of neurotoxicants (acrylamide, carbon disulfide (CS2), 2,5-hexanedione (2,5-HD)), to cytoskeletal proteins produces a dying back type of neuropathy...
  24. IMAGING OF THREE-DIMENSIONAL DOSE DISTRIBUTIONS NMR
    Robert Schulz; Fiscal Year: 1990
    ..phantoms from x-ray and electron beams and brachytherapy sources; f) explore other radiation-induced chemical reactions, such as the polymerization of acrylamide, that affect T1 but do not require the addition of Fricke dosimeter.
  25. TRANSIENT EFFECTS IN FLUORESCENCE QUENCHING
    Joseph Lakowicz; Fiscal Year: 1991
    ..effects we will examine short and longer-lived probes in viscous and non-viscous solvents, quenched by oxygen, acrylamide, and halogenated quenchers...
  26. GENE VARIATION IN NATURAL POPULATIONS
    Satya Prakash; Fiscal Year: 1980
    ..Structural variants at these loci will be distinguished by acrylamide gel electrophoresis of proteins and enzymes in gels of different concentrations, different pH's and by ..
  27. COLLAGEN METABOLISM IN RAT LUNG EXPLANTS
    Deborah Greenberg; Fiscal Year: 1980
    ..The different types of collagen present will be separated and quantitated, using acrylamide gel electrophoresis, ion-exchange chromatography and cyanogen bromide fragmentation of peptides...
  28. PROPERTIES OF THE HIGH-AFFINITY INSULIN RECEPTOR
    Michael Czech; Fiscal Year: 1980
    ..Since we can follow the size of the labelled receptor on acrylamide gels by autoradiography, time- and temperature-dependent proteolysis or convalent associations with other ..
  29. RENAL WATER AND ION TRANSPORT: ROLE OF PROTEINS
    IRWIN SINGER; Fiscal Year: 1980
    ..osmotic water flow, isotope flux, radiotracer- and fluorescence-labelling of proteins, gel chromatography, acrylamide gel electrophoresis and autoradiography...
  30. REGULATION OF IMMUNITY AT THE MOLECULAR LEVEL
    Howard Dintzis; Fiscal Year: 1980
    ..vivo immunological response was measured in mice injected with a series of size fractionated linear polymers of acrylamide substituted with hapten, this project proposes: 1...
  31. IMRT FOR GYNECOLOGICAL MALIGNANCIES
    Daniel Low; Fiscal Year: 2002
    ..Two multidimensional dosimeters, Radiochromic film and BANG (Bis, Acrylamide, Nitrogen, and Gelatin) gel, will be benchmarked and calibrated to measure the expected steep dose gradients of ..
  32. A new method to express siRNA
    Zhongting Hu; Fiscal Year: 2005
    ..To detect expressing siRNA in cells more efficiently, we have also designed a new acrylamide-gel based isolation system that can enrich siRNAs (or any small RNA molecules) easily, quickly and with high ..
  33. MECHANISM OF PHOSPHOLIPASE A2
    Ming Daw Tsai; Fiscal Year: 2000
    ..that binding of PLA2 to the interface makes Trp-3 inaccessible to fluorescence quenching by water-soluble acrylamide or succinimide...
  34. NEURONAL PERIKARYAL RESPONSES TO NEUROTOXIC CHEMICALS
    BRUCE GOLD; Fiscal Year: 1990
    ..Previous studies following chronic acrylamide administration, which produces axonal degeneration, appear to support the latter possibility...
  35. IMMUNOCONTRACEPTION: ANTIBODIES TO ZONA PELLUCIDA
    ANTHONY SACCO; Fiscal Year: 1990
    ..isolated using standard chromatographic and HPLC methodology and homogeneity verified by one-and-two dimensional acrylamide gel electrophoresis...
  36. DETECTION AND ISOLATION OF HUMAN BLADDER ANTIGENS
    David Paulson; Fiscal Year: 1980
    ..Immunological identification of separated proteins will be by migration of the acrylamide-separated proteins into agarose containing antihuman bladder sera and by radioimmunoassay (RIA) against ..
  37. MOLECULAR MECHANISMS OF HEXACARBON-INDUCED AXON ATROPHY
    RICHARD LOPACHIN; Fiscal Year: 2009
    ..Understanding the role and mechanism of axon atrophy in solvent neurotoxicity has broad-based implications for human occupational health and risk assessment. ..
  38. IMMUNOLOGIC PROTECTION AGAINST MALARIA
    ROBERT REESE; Fiscal Year: 1980
    ..sera from humans, monkeys and rabbits have been studied using immunofluorescence, immunoelectron microscopy, SDS acrylamide gel electrophoresis and the inhibition of the growth of Plasmodium falciparum in culture...
  39. DETECTION OF NEUROTOXICITY OF ENVIRONMENTAL AGENTS
    Rebecca Anderson; Fiscal Year: 1980
    ..Cats and rats will be administered acrylamide or polychlorinated biphenyls, two known neurotoxic agents, in order to evaluate the sensitivity and selectivity ..
  40. CONSEQUENCES OF PROXIMAL GIANT AXON FORMATIONS
    HERBERT LOWNDES; Fiscal Year: 1992
    ..Primary afferent terminal function will be investigated in cats with neuropathies included by acrylamide or doxorubicin...
  41. RAPID, HIGHLY EFFICIENT SUBTRACTION CDNA CLONING METHOD
    WILLIAM MACCONNELL; Fiscal Year: 1999
    ..novel and powerful new hybridization technology in which nucleic acids are electrophoresed through boundaries of acrylamide which have covalently bound DNA capture DNA probes...
  42. COMPACT, BUFFERLESS GELS FOR CLINICAL APPLICATIONS
    WILLIAM MACCONNELL; Fiscal Year: 1992
    ..abstract):The present proposal pertains to the development of an inexpensive, disposable device for rapid acrylamide or agarose gel electrophoretic separation of DNA, RNA or protein which does not require running buffer or user ..
  43. METABOLISM OF INDUSTRIAL TOXICANTS
    KENNETH LEIBMAN; Fiscal Year: 1980
    ..This includes studies of the relationship of biochemical toxicology of acrolein to its biotransformation, the mechanism of cyanide release from acrylonitrile, and the metabolism of acrylamide.
  44. PEROXIDASE IN NORMAL AND NEOPLASTIC MELANOGENESIS
    MILTON OKUN; Fiscal Year: 1980
    ..Enzymes will be purified by column chromatography, and purity of enzymes will be tested, using acrylamide gel electrophoresis...
  45. ANTIGENIC DETERMINANTS OF VARICELLA VIRUS
    Charles Grose; Fiscal Year: 1980
    ..on propagation and purification of varicella virus and then includes a major section on characterization by acrylamide gel electrophoresis...
  46. CHARACTERIZATION OF THE B PROTEINS OF SERUM LIPOPROTEINS
    Karen Kuehl; Fiscal Year: 1980
    ..protein of human low density lipoprotein exhibits heterogeniety upon SDS PAGE in gels containing a gradient of acrylamide. Quantitative yields of amino and carboxyl termini also suggest that the protein is a multimer of much lower ..
  47. DETECTING DNA DAMAGE--INDIVIDUAL GERM CELL
    ROLLAND POUST; Fiscal Year: 1990
    ..will be on the various methods used to isolate germ cells from the testis, and the kinetics of the induction of DNA damage induced by selected mutagenic agents (acrylamide, cis-platinum, cyclophosphamide, and methylmethansulphonate).
  48. LC-MS/MS Methods for Analysis of Hemoglogbin Adducts
    Timothy Fennell; Fiscal Year: 2004
    ..Preliminary studies conducted in our laboratory indicate that the analysis of hemoglobin adducts of acrylamide and glycidamide released by a modified Edman degradation can be accomplished by liquid chromatraphy-mass ..
  49. Role of Kupffer Cell in Hepatic Carcinogenesis
    James Klaunig; Fiscal Year: 2009
    ..These studies will further our understanding into the mechanisms of the development and progression of human liver cancer with potential application to prevention and therapy. ..
  50. SEROTONERGIC REGULATION OF SENSORY TRANSMISSION
    Min Zhuo; Fiscal Year: 2002
    ..The proposed studies will generate synaptic mechanisms for fast glutamatergic transmission as well as 5-HT regulation in the spinal cord. This information will be important for understanding central pain transmission and modulation. ..
  51. NEW BIOMATERIALS TO MINIMIZE SCAR TISSUE FORMATION
    Joan Sanders; Fiscal Year: 2002
    ..e. soft-tissue complications that require surgical repair through use of a biomaterial. The new materials will prevent scar tissue formation and reduce the occurrence of secondary complications. ..
  52. STRUCTURES OF ACETYLCHOLINE RECEPTORS
    Arthur Karlin; Fiscal Year: 2003
    ..The Cys will be substituted for residues known to contribute to the binding of ACh. ..
  53. Structure and Function of Apolipoproteins
    JOSE SOULAGES; Fiscal Year: 2007
    ..This includes understanding of the role of apolipoproteins in the metabolism of lipids and related pathologies, such as atherosclerosis, but it could also involve other kind of pathologies, such as Alzheimer's disease. ..
  54. INTERFACE MECHANICS, MATERIALS AND TISSUE RESPONSE
    Joan Sanders; Fiscal Year: 2007
    ..The development of methods to predict and overcome the detrimental effects of residual limb shape change and ultimately prevent soft-tissue breakdown will help to prevent secondary disability and morbidity in the amputee population. ..
  55. Development of (Non) Invasive Real-Time Glucose Sensors
    Sanford Asher; Fiscal Year: 2005
    ..abstract_text> ..