2 acetylaminofluorene

Summary

Summary: A hepatic carcinogen whose mechanism of activation involves N-hydroxylation to the aryl hydroxamic acid followed by enzymatic sulfonation to sulfoxyfluorenylacetamide. It is used to study the carcinogenicity and mutagenicity of aromatic amines.

Top Publications

  1. ncbi Identification of adult hepatic progenitor cells capable of repopulating injured rat liver
    Mladen I Yovchev
    Marion Bessin Liver Research Center, Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Hepatology 47:636-47. 2008
  2. ncbi Novel hepatic progenitor cell surface markers in the adult rat liver
    Mladen I Yovchev
    Marion Bessin Liver Research Center, Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Hepatology 45:139-49. 2007
  3. ncbi Localization of DNA repair synthesis by human cell extracts to a short region at the site of a lesion
    J Hansson
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, United Kingdom
    J Biol Chem 264:21788-92. 1989
  4. ncbi Bone marrow as a potential source of hepatic oval cells
    B E Petersen
    Department of Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Science 284:1168-70. 1999
  5. ncbi Mechanism for N-acetyl-2-aminofluorene-induced frameshift mutagenesis by Escherichia coli DNA polymerase I (Klenow fragment)
    Joshua P Gill
    Department of Chemistry, Wayne State University, Detroit, Michigan 48202, USA
    Biochemistry 44:15387-95. 2005
  6. pmc Site-specific incorporation of N-(deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-AAF) into oligonucleotides using modified 'ultra-mild' DNA synthesis
    Ludovic C J Gillet
    Institute for Molecular Cancer Research, University of Zürich August Forel Strasse 7, 8008 Zurich, Switzerland
    Nucleic Acids Res 33:1961-9. 2005
  7. ncbi Recognition of DNA adducts by human nucleotide excision repair. Evidence for a thermodynamic probing mechanism
    D Gunz
    Institute of Pharmacology and Toxicology, University of Zurich Tierspital, Winterthurerstrasse 260, 8057 Zurich, Switzerland
    J Biol Chem 271:25089-98. 1996
  8. ncbi Mutagenic events in Escherichia coli and mammalian cells generated in response to acetylaminofluorene-derived DNA adducts positioned in the Nar I restriction enzyme site
    Xingzhi Tan
    Laboratory of Chemical Biology, Department of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, New York 11794 8651, USA
    Biochemistry 41:14255-62. 2002
  9. pmc Crystal structures of 2-acetylaminofluorene and 2-aminofluorene in complex with T7 DNA polymerase reveal mechanisms of mutagenesis
    Shuchismita Dutta
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 101:16186-91. 2004
  10. pmc Role of connective tissue growth factor in oval cell response during liver regeneration after 2-AAF/PHx in rats
    Liya Pi
    Department of Pathology, Immunology and Laboratory Medicine, University of Florida, College of Medicine, Gainesville 32610 0275, USA
    Gastroenterology 128:2077-88. 2005

Research Grants

Detail Information

Publications217 found, 100 shown here

  1. ncbi Identification of adult hepatic progenitor cells capable of repopulating injured rat liver
    Mladen I Yovchev
    Marion Bessin Liver Research Center, Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Hepatology 47:636-47. 2008
    ..It is shown that Thy-1(+) cells are mesenchymal cells with characteristics of myofibroblasts/activated stellate cells. Transplantation experiments reveal that EpCAM(+) cells are true progenitors capable of repopulating injured rat liver...
  2. ncbi Novel hepatic progenitor cell surface markers in the adult rat liver
    Mladen I Yovchev
    Marion Bessin Liver Research Center, Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Hepatology 45:139-49. 2007
    ..Moreover, study of progenitor cells purified with Ep-CAM antibodies from D-galactosamine injured rat liver, a noncarcinogenic model of progenitor cell activation, verified that progenitor cells expressed these markers...
  3. ncbi Localization of DNA repair synthesis by human cell extracts to a short region at the site of a lesion
    J Hansson
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, United Kingdom
    J Biol Chem 264:21788-92. 1989
    ..DNA repair synthesis induced by the 2-(acetyl-amino)fluorene adduct took place in the damaged strand and was confined to a region within a 31-base pair restriction fragment around the adduct...
  4. ncbi Bone marrow as a potential source of hepatic oval cells
    B E Petersen
    Department of Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Science 284:1168-70. 1999
    ..From these cells, a proportion of the regenerated hepatic cells were shown to be donor-derived. Thus, a stem cell associated with the bone marrow has epithelial cell lineage capability...
  5. ncbi Mechanism for N-acetyl-2-aminofluorene-induced frameshift mutagenesis by Escherichia coli DNA polymerase I (Klenow fragment)
    Joshua P Gill
    Department of Chemistry, Wayne State University, Detroit, Michigan 48202, USA
    Biochemistry 44:15387-95. 2005
    ....
  6. pmc Site-specific incorporation of N-(deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-AAF) into oligonucleotides using modified 'ultra-mild' DNA synthesis
    Ludovic C J Gillet
    Institute for Molecular Cancer Research, University of Zürich August Forel Strasse 7, 8008 Zurich, Switzerland
    Nucleic Acids Res 33:1961-9. 2005
    ..Our synthetic approach should facilitate comprehensive studies of the mechanisms of repair and mutagenesis induced by dG-AAF adducts in DNA and should be of general use for the incorporation of base-labile functionalities into DNA...
  7. ncbi Recognition of DNA adducts by human nucleotide excision repair. Evidence for a thermodynamic probing mechanism
    D Gunz
    Institute of Pharmacology and Toxicology, University of Zurich Tierspital, Winterthurerstrasse 260, 8057 Zurich, Switzerland
    J Biol Chem 271:25089-98. 1996
    ..Thus, an early step of DNA damage recognition involves thermodynamic probing of the duplex...
  8. ncbi Mutagenic events in Escherichia coli and mammalian cells generated in response to acetylaminofluorene-derived DNA adducts positioned in the Nar I restriction enzyme site
    Xingzhi Tan
    Laboratory of Chemical Biology, Department of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, New York 11794 8651, USA
    Biochemistry 41:14255-62. 2002
    ..3-21%, the higher value at G(2). We conclude from this study that the mutation potential of dG-AAF and dG-AF depends on the structure of the adduct, the sequence context of the lesion, and the host cell used for the experiment...
  9. pmc Crystal structures of 2-acetylaminofluorene and 2-aminofluorene in complex with T7 DNA polymerase reveal mechanisms of mutagenesis
    Shuchismita Dutta
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 101:16186-91. 2004
    ..In contrast, the dG-AF base at the templating position is not well defined by the electron density, consistent with weak binding to the polymerase and a possible interchange of this adduct between the syn and anti conformations...
  10. pmc Role of connective tissue growth factor in oval cell response during liver regeneration after 2-AAF/PHx in rats
    Liya Pi
    Department of Pathology, Immunology and Laboratory Medicine, University of Florida, College of Medicine, Gainesville 32610 0275, USA
    Gastroenterology 128:2077-88. 2005
    ..To understand the molecular mechanism underlying this process, we investigated the role of connective tissue growth factor (CTGF), one of the candidate genes differentially expressed in Thy-1+ oval cells, in this liver injury model...
  11. pmc Treatment with 2-AAF blocks the small hepatocyte-like progenitor cell response in retrorsine-exposed rats
    D Hunter Best
    Department of Pathology and Laboratory Medicine, Curriculum in Toxicology, UNC Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    J Hepatol 46:1055-63. 2007
    ....
  12. ncbi Bone marrow-derived hepatic oval cells differentiate into hepatocytes in 2-acetylaminofluorene/partial hepatectomy-induced liver regeneration
    Seh Hoon Oh
    Department of Pathology, Immunology and Laboratory Medicine, University of Florida College of Medicine, Gainesville, Florida 32610, USA
    Gastroenterology 132:1077-87. 2007
    ..The present study analyzed whether the bone marrow cells are a source of hepatic oval cells involved in rat liver regeneration induced by 2-acetylaminofluorene (2-AAF) and 70% partial hepatectomy (PHx)...
  13. ncbi Influence of flanking sequence context on the mutagenicity of acetylaminofluorene-derived DNA adducts in mammalian cells
    S Shibutani
    Laboratory of Chemical Biology, Department of Pharmacological Sciences, State University of New York at Stony Brook, 11794 8651, USA
    Biochemistry 40:3717-22. 2001
    ..The mutational frequency and specificity of dG-AF vary significantly, depending on the nature of the bases flanking the lesion...
  14. ncbi Bone marrow progenitors are not the source of expanding oval cells in injured liver
    Anuradha Menthena
    Marion Bessin Liver Research Center, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Stem Cells 22:1049-61. 2004
    ..These data demonstrate that the sources of oval cells and small hepatocytes in the injured liver are endogenous liver progenitors and that they do not arise through transdifferentiation from BM cells...
  15. pmc Translesion synthesis of acetylaminofluorene-dG adducts by DNA polymerase zeta is stimulated by yeast Rev1 protein
    Dongyu Guo
    Graduate Center for Toxicology, University of Kentucky, Lexington, KY 40536, USA
    Nucleic Acids Res 32:1122-30. 2004
    ..Consistent with the in vitro results, both Polzeta and Rev1 were found to be equally important for error-prone translesion synthesis across from AAF-dG DNA adducts in yeast cells...
  16. ncbi Mutagenic specificity of (acetylamino)fluorene-derived DNA adducts in mammalian cells
    S Shibutani
    Department of Pharmacological Sciences, State University of New York at Stony Brook 11794 8651, USA
    Biochemistry 37:12034-41. 1998
    ..dG-AAF was 3.8 times more mutagenic than dG-AF (11% vs 2.9%) in COS cells. We conclude from this study that dG-AAF and dG-AF produce G --> T transversions and, to a much lesser degree, G --> A transitions in mammalian cells...
  17. ncbi Translesional synthesis past acetylaminofluorene-derived DNA adducts catalyzed by human DNA polymerase kappa and Escherichia coli DNA polymerase IV
    N Suzuki
    Laboratory of Chemical Biology, Department of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, New York 11794-8651, USA
    Biochemistry 40:15176-83. 2001
    ..et al. (2001) Biochemistry 40, 3717-3722], supporting a model in which pol kappa plays a role in translesion synthesis past acetylaminofluorene-derived lesions in mammalian cells...
  18. pmc Origin and structural evolution of the early proliferating oval cells in rat liver
    S Paku
    Joint Research Organization of the Hungarian Academy of Sciences and Semmelweis University of Medicine, Budapest, Hungary
    Am J Pathol 158:1313-23. 2001
    ....
  19. ncbi Mutagenic properties of 3-(deoxyguanosin-N2-yl)-2-acetylaminofluorene, a persistent acetylaminofluorene-derived DNA adduct in mammalian cells
    Manabu Yasui
    Laboratory of Chemical Biology, Department of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, New York 11794 8651, USA
    Biochemistry 43:15005-13. 2004
    ....
  20. ncbi Mechanism of frameshift (deletion) generated by acetylaminofluorene-derived DNA adducts in vitro
    Shinya Shibutani
    Laboratory of Chemical Biology, Department of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, New York 11794 8651, USA
    Biochemistry 43:15929-35. 2004
    ..Taken together, these results support the molecular mechanism for frameshift deletion proposed earlier by Shibutani and Grollman in which direct base insertion precedes misalignment [(1993) J. Biol. Chem. 268, 11703]...
  21. ncbi Induction of mutations by N-acetoxy-N-acetyl-2-aminofluorene modified M13 viral DNA
    P K Gupta
    Department of Chemical Carcinogenesis, Michigan Cancer Foundation, Detroit 48201
    Carcinogenesis 12:819-24. 1991
    ..Molecular mechanisms for the induction of these mutations by DNA perturbations produced by the G-8-AAF adducts are discussed...
  22. pmc Pleiotrophin/heparin-binding growth-associated molecule as a mitogen of rat hepatocytes and its role in regeneration and development of liver
    Kinji Asahina
    Hiroshima Tissue Regeneration Project, Hiroshima Prefecture Collaboration of Regional Entities for the Advancement of Technological Excellence, Japan Science and Technology Corporation, Hiroshima Prefectural Institute of Industrial Science and Technology
    Am J Pathol 160:2191-205. 2002
    ..We suggest that PTN is secreted from activated adult HSCs and embryonic mesenchymal cells as a mitogen of parenchymal cells in adult and embryonic liver, respectively...
  23. ncbi Characterization of cytokeratin 19-positive hepatocyte foci in the regenerating rat liver after 2-AAF/CCl4 injury
    Chien Chang Chiu
    The Graduate Institute of Applied Science and Engineering, Fu Jen Catholic University, No 510 Chung Cheng Rd, Hsinchuang, Taipei County, 24205, and Department of Internal Medicine, National Taiwan University Hospital, Taiwan
    Histochem Cell Biol 128:217-26. 2007
    ..In conclusions, oval cells appear to differentiate to CK-19+ foci and then to CK-19- foci in the high dose 2-AAF/CCl4 model...
  24. ncbi Vanadium suppresses sister-chromatid exchange and DNA-protein crosslink formation and restores antioxidant status and hepatocellular architecture during 2-acetylaminofluorene-induced experimental rat hepatocarcinogenesis
    Tridib Chakraborty
    Division of Biochemistry, Department of Pharmaceutical Technology, Jadavpur University, Calcutta, West Bengal, India
    J Exp Ther Oncol 3:346-62. 2003
    ....
  25. ncbi Activation of NF-kappaB and STAT3 in rat oval cells during 2-acetylaminofluorene/partial hepatectomy-induced liver regeneration
    Aránzazu Sánchez
    Laboratory of Experimental Carcinogenesis, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Hepatology 39:376-85. 2004
    ..The differential induction of NF-kappaB and STAT3 point to a distinct role for these transcription factors at different stages of hepatic stem cell differentiation...
  26. pmc Granulocyte-colony stimulating factor promotes liver repair and induces oval cell migration and proliferation in rats
    Anna C Piscaglia
    Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, Florida 32610, USA
    Gastroenterology 133:619-31. 2007
    ..In the present study, we assessed the involvement of G-CSF during oval cell activation...
  27. ncbi Inhibition of liver carcinogenesis in Wistar rats by consumption of an aqueous extract from leaves of Ardisia compressa
    E Gonzalez de Mejia
    Department of Food Science and Human Nutrition, University of Illinois, Urbana Champaign, 228 ERML, M C 051, 1201 W Gregory Drive, Urbana, IL 61801, USA
    Food Chem Toxicol 42:509-16. 2004
    ..01). The striking observation of this study was that consumption of A. compressa tea resulted in complete inhibition of the chemically-induced hepatocarcinogenesis in Wistar rats...
  28. ncbi Cloning and expression of the catalytic domain from rat hepatoma H35 cell GDP-fucose:GM1 alpha 1-->2fucosyltransferase, an enzyme which is activated during early stages of chemical carcinogenesis in rat liver
    A L Sherwood
    Department of Cell Surface Biochemistry, Northwest Hospital, Pacific Northwest Cancer Foundation, 120 Northgate Plaza, Suite 218, Seattle, Washington, 98125, USA
    Arch Biochem Biophys 355:215-21. 1998
    ..The H35 cell alpha 1-->2fucosyltransferase was expressed in the pPROTA vector and the derived fusion protein demonstrated the ability to transfer fucose to ganglioside GM1 but not to the neolacto-series acceptor nLcOse4Cer...
  29. pmc A single dose of caffeic acid phenethyl ester prevents initiation in a medium-term rat hepatocarcinogenesis model
    Claudia Esther Carrasco-Legleu
    Departamento de Biologia Celular, Laboratorio 50, Centro de Investigación y de Estudios Avanzados del IPN Cinvestav Ave IPN 2508 Col San Pedro Zacatenco, C P 07360, Mexico, D F, Mexico
    World J Gastroenterol 12:6779-85. 2006
    ..To study of the effect of caffeic acid phenethyl ester (CAPE) on the initiation period in a medium-term assay of hepatocarcinogenesis...
  30. ncbi Critique of dose response in carcinogenesis
    W J Waddell
    Department of Pharmacology and Toxicology, School of Medicine, University of Louisville, KY, USA
    Hum Exp Toxicol 25:413-36. 2006
    ..Experiments showing that cumulative dose is a better metric than daily dose may require reevaluating almost all carcinogenicity studies...
  31. ncbi Initiating activity of 4-chlorobiphenyl metabolites in the resistant hepatocyte model
    Parvaneh Espandiari
    Graduate Center for Toxicology, University of Kentucky Medical Center, Lexington, Kentucky 40536, USA
    Toxicol Sci 79:41-6. 2004
    ..The results support the conclusion that 4-OH PCB 3 and 3,4-BQ PCB 3 act as proximate and ultimate carcinogenic metabolites resulting from the bioactivation of PCB 3 in rat liver...
  32. ncbi In vivo cell lineage analysis during chemical hepatocarcinogenesis in rats using retroviral-mediated gene transfer: evidence for dedifferentiation of mature hepatocytes
    Jerome Gournay
    Laboratoire de Thérapie Génique, Hotel Dieu, Centre Hospitalier Universitaire de Nantes, Nantes, France
    Lab Invest 82:781-8. 2002
    ..These results demonstrate that preneoplastic foci can originate from mature hepatocytes and are consistent with the hypothesis that dedifferentiation of mature hepatocytes may occur during the course of carcinogenic regimen...
  33. ncbi Zonal hierarchy of differentiation markers and nestin expression during oval cell mediated rat liver regeneration
    Sarah Koenig
    Department of General Surgery, University Hospital, Robert Koch Strasse 40, 37099 Goettingen, Germany
    Histochem Cell Biol 126:723-34. 2006
    ....
  34. ncbi Inhibition by acetaminophen of neoplastic initiation elicited in rat liver by the DNA-reactive hepatocarcinogen N-acetyl-2-aminofluorene
    Gary M Williams
    Department of Pathology, New York Medical College, Valhalla, New York, USA
    Eur J Cancer Prev 16:528-34. 2007
    ..Thus, acetaminophen effectively protected hepatocytes from the initiating effects of N-acetyl-2-aminofluorene, possibly through a cytoprotective effect resulting from slowing the rate of induced cell turnover...
  35. ncbi Thy-1 is an in vivo and in vitro marker of liver myofibroblasts
    Jozsef Dudas
    Department of Internal Medicine, Section of Gastroenterology and Endocrinology, Georg August University Gottingen, Robert Koch Strasse 40, 37075 Gottingen, Germany
    Cell Tissue Res 329:503-14. 2007
    ..Thy-1 is the first cell surface marker for identifying liver myofibroblasts in vivo and in vitro...
  36. ncbi Induction of preneoplastic altered hepatic foci following dietary sulphur supplementation
    Annu Arora
    Environmental Carcinogenesis Division, Industrial Toxicology Research Centre, M G Marg PO Box No 80, Lucknow 226 001, India
    Hum Exp Toxicol 23:229-34. 2004
    ..However, 1% sulphur administration failed to induce AHF up to significant levels. The results thus revealed the possible tumorigenic risk associated with the high sulphur-containing diet...
  37. ncbi Embryonic turkey liver: activities of biotransformation enzymes and activation of DNA-reactive carcinogens
    Carmen E Perrone
    Department of Pathology, New York Medical College, Valhalla, NY 10595, USA
    Arch Toxicol 78:589-98. 2004
    ..Thus, turkey embryos could be utilized to investigate potential chemical toxicity and carcinogenicity...
  38. ncbi 2-acetylaminofluorene dose-dependent differentiation of rat oval cells into hepatocytes: confocal and electron microscopic studies
    Sandor Paku
    Joint Research Organization of the Hungarian Academy of Sciences and Semmelweis University, Budapest, Hungary
    Hepatology 39:1353-61. 2004
    ..In conclusion, these results indicate the rate of oval cell differentiation into hepatocytes is context dependent and suggest that, under favorable conditions, oval cells can complete this process much faster than previously appreciated...
  39. ncbi The Liverbeads as a tool for the comet assay
    Laurence Vian
    Laboratoire de Toxicologie, Faculte de Pharmacie, BP 14491, 34093 Montpellier Cedex 5, France
    Mutat Res 519:163-70. 2002
    ..Due to the maintenance of specific functions assessed by the observed capacity to metabolize xenobiotics, Liverbeads represent a suitable tool system, easy to handle, for the detection of promutagens using the comet assay...
  40. ncbi Effect of cacao liquor extract on tumor marker enzymes during chemical hepatocarcinogenesis in rats
    I Amin
    Department of Nutrition and Health Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor Darul Ehsan, Malaysia
    J Med Food 7:7-12. 2004
    ..Based on the results obtained, polyphenol compounds present in the cacao liquor, extracted by using ethanol, have the potential in decreasing the severity of hepatocarcinogenesis...
  41. ncbi Pregnane X receptor-dependent and -independent effects of 2-acetylaminofluorene on cytochrome P450 3A23 expression and liver cell proliferation
    Lydie Sparfel
    INSERM U456, Faculte de Pharmacie, 2 Avenue du Pr Leon Bernard, Rennes 35043, France
    Biochem Biophys Res Commun 300:278-84. 2003
    ..Such data indicate that AAF up-regulates CYP3A23 through PXR activation but does not require PXR for exerting its carcinogenic promoting properties based on inhibition of cell growth...
  42. pmc Early resistance to cell death and to onset of the mitochondrial permeability transition during hepatocarcinogenesis with 2-acetylaminofluorene
    Peter Christian Klohn
    Department of Biomedical Sciences, University of Padua, Viale Giuseppe Colombo 3, I 35121 Padua, Italy
    Proc Natl Acad Sci U S A 100:10014-9. 2003
    ..Both events precede tumor formation, suggesting that suppression of apoptosis may contribute to the selection of a resistant phenotype, eventually increasing the probability of cell progression to the transformed state...
  43. ncbi Vitamin A and all-trans and 9-cis retinoic acids inhibit cell proliferation during the progression phase of hepatocarcinogenesis in Wistar rats
    E R Silveira
    Laboratory of Diet, Nutrition, and Cancer, Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, , , Brazil
    Nutr Cancer 39:244-51. 2001
    ..Therefore, the anticarcinogenic effects that have been attributed to these retinoids could be partially related to their capacity of inhibiting in vivo cell proliferation...
  44. ncbi Prevalidation of a rat liver foci bioassay (RLFB) based on results from 1600 rats: a study report
    Carina Ittrich
    Central Unit Biostatistics, German Cancer Research Center DKFZ, P O Box 101949, D 69009 Heidelberg, Germany
    Toxicol Pathol 31:60-79. 2003
    ..Our results suggest that this RLFB is a sensitive bioassay, providing potentially valuable information for risk assessment including the classification of carcinogenic chemicals according to their mode of action...
  45. ncbi 1Alpha,25-dihydroxyvitamin D3 inhibits hepatic chromosomal aberrations, DNA strand breaks and specific DNA adducts during rat hepatocarcinogenesis
    B K Saha
    Department of Pharmaceutical Technology, Jadavpur University, Calcutta, India
    Cell Mol Life Sci 58:1141-9. 2001
    ....
  46. ncbi Abnormalities in liver iron accumulation during N-2-fluorenylacetamide hepatocarcinogenesis that are dependent or independent of continued carcinogen action
    K Furuya
    Naylor Dana Institute for Disease Prevention, American Health Foundation, Valhalla, NY 10595, USA
    Toxicol Pathol 12:136-42. 1984
    ..Altered foci appear to represent two populations: one is characterized by reversible iron-exclusion whereas the other, like neoplasms, possesses permanent iron-exclusion...
  47. pmc Oval cell response in 2-acetylaminofluorene/partial hepatectomy rat is attenuated by short interfering RNA targeted to stromal cell-derived factor-1
    Donghang Zheng
    Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, P O Box 100275, Gainesville, FL 32610 0275, USA
    Am J Pathol 169:2066-74. 2006
    ..These results indicate that SDF-1 is an essential molecule needed in oval cell activation...
  48. ncbi Characterization and enrichment of hepatic progenitor cells in adult rat liver
    Ai Lan Qin
    Department of Infectious Diseases, Ruijin Hospital, Shanghai 200025, China
    World J Gastroenterol 10:1480-6. 2004
    ..To detect the markers of oval cells in adult rat liver and to enrich them for further analysis of characterization in vitro...
  49. pmc Hepatic oval cells have the side population phenotype defined by expression of ATP-binding cassette transporter ABCG2/BCRP1
    Koichi Shimano
    Department of Pathology, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan
    Am J Pathol 163:3-9. 2003
    ..These results indicate that oval cells have the SP phenotype defined by expression of ABCG2/BCRP1, suggesting that oval cells may represent stem cells in the liver...
  50. ncbi Inhibition by dietary hydroquinone of acetylaminofluorene induction of initiation of rat liver carcinogenesis
    Gary M Williams
    Department of Pathology, Basic Science Building, New York Medical College, Valhalla, NY 10595, USA
    Food Chem Toxicol 45:1620-5. 2007
    ..Thus, under the conditions of this experiment, HQ at both 0.05% and 0.2% in the diet diminished AAF-induced cancer initiating effects in rat liver...
  51. ncbi Persistent and remodeling hepatic preneoplastic lesions present differences in cell proliferation and apoptosis, as well as in p53, Bcl-2 and NF-kappaB pathways
    Rogério Pietro Mazzantini
    Laboratory of Diet, Nutrition and Cancer, Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil
    J Cell Biochem 103:538-46. 2008
    ..These data point to molecular pathways that may direct preneoplastic lesions to spontaneously regress or to progress to cancer. J. Cell. Biochem. 103: 538-546, 2008. (c) 2007 Wiley-Liss, Inc...
  52. ncbi Thresholds for the effects of 2-acetylaminofluorene in rat liver
    Gary M Williams
    New York Medical College, Department of Pathology, Valhalla, New York 10595, USA
    Toxicol Pathol 32:85-91. 2004
    ..Thus, mechanisms of carcinogenesis can differ between low and high doses...
  53. ncbi Evidence that the anticarcinogenic effect of caffeic acid phenethyl ester in the resistant hepatocyte model involves modifications of cytochrome P450
    Olga Beltràn-Ramírez
    Departamento de Biologia Celular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional CINVESTAV, Avenida IPN No 2508 Colonia San Pedro Zacatenco, Mexico 14, DF, CP 07360, Mexico
    Toxicol Sci 104:100-6. 2008
    ..These findings describe an alternative mechanism for understanding the ability of CAPE to protect against chemical hepatocarcinogenesis...
  54. ncbi The amoebicidal aqueous extract from Castela texana possesses antigenotoxic and antimutagenic properties
    M Reyes-López
    Departamento de Biologia Celular, Centro de Investigación y de Estudios Avanzados del IPN Apartado postal 14 740 México D F 07000, Mexico
    Toxicol In Vitro 19:91-7. 2005
    ..texana, is also antimutagenic and protects against induction of preneoplastic lesions in rat liver. These results justify further studies to extend it use to human beings...
  55. ncbi Hepatocytes isolated from preneoplastic rat livers are resistant to ethacrynic acid cytotoxicity
    Juan Pablo Parody
    Facultad de Ciencias Bioquímicas y Farmacéuticas UNR, Instituto de Fisiología Experimental IFISE CONICET, Suipacha 570, 2000 Rosario, Santa Fe, Argentina
    Arch Toxicol 81:565-73. 2007
    ..In conclusion, increased total GSH, decreased Mrp2 levels and the presence of GST P could be critical factors involved in the resistance of IP hepatocytes to the toxicity of EA...
  56. ncbi Hepatocyte growth factor accelerates the proliferation of hepatic oval cells and possibly promotes the differentiation in a 2-acetylaminofluorene/partial hepatectomy model in rats
    Satoru Hasuike
    Department of Internal Medicine II, Faculty of Medicine, University of Miyazaki, Kihara, Kiyotake, Miyazaki, Japan
    J Gastroenterol Hepatol 20:1753-61. 2005
    ..The purpose of the present paper was to clarify the effects of HGF on the proliferation and differentiation of hepatic oval cells using a 2-acetylaminofluorene/partial hepatectomy (2-AAF/PH) model in rats...
  57. ncbi Comparison of different initiation protocols in the resistant hepatocyte model
    Parvaneh Espandiari
    Graduate Center for Toxicology, University of Kentucky, Lexington, KY 40506, USA
    Toxicology 206:373-81. 2005
    ..We conclude that the use of male neonatal rats can replace the PH in the Solt-Farber protocol. Fasting/refeeding was also effective at increasing the volume fraction of GGT-positive foci, but only at the higher dose of DEN...
  58. ncbi Identification of genes specific to "oval cells" in the rat 2-acetylaminofluorene/partial hepatectomy model
    Danko S Batusic
    Department of Internal Medicine, Section of Gastroenterology and Endocrinology, Georg August University, Gottingen, 37099, Germany
    Histochem Cell Biol 124:245-60. 2005
    ..We postulate that Ear-2 and Rab-3b may represent novel regulatory factors specifically activated in "oval cells"...
  59. ncbi Comparisons of thresholds for carcinogenicity on linear and logarithmic dosage scales
    William J Waddell
    Department of Pharmacology and Toxicology, School of Medicine, University of Louisville, Louisville, KY, USA
    Hum Exp Toxicol 24:325-32. 2005
    ..It is suggested that the high background incidence of tumours in the BALB/c StCrlfC3Hf/Nctr mouse liver may confound the interpretation of dose response from AAF carcinogenesis in mouse liver...
  60. ncbi Inhibitory effects of KAT-681, a liver-selective thyromimetic, on development of hepatocellular proliferative lesions in rats induced by 2-acetylaminofluorene and partial hepatectomy after diethylnitrosamine initiation
    Morimichi Hayashi
    Toxicology Laboratories, Research and Development, Kissei Pharmaceutical Co Ltd, 2320 1 Maki, Hotaka, Minamiazumi gun, 399 8305 Nagano, Japan
    Arch Toxicol 78:460-6. 2004
    ..25 mg/kg per day KAT (by 64%). The results of the present study thus suggest that KAT inhibits the development of altered hepatocellular foci and might be a promising chemopreventive agent for hepatocarcinogenesis...
  61. ncbi Prospero-related homeobox 1 (Prox1) is a stable hepatocyte marker during liver development, injury and regeneration, and is absent from "oval cells"
    Jozsef Dudas
    Department of Internal Medicine, Section of Gastroenterology and Endocrinology, Georg August University Gottingen, Robert Koch Strasse 40, 37075 Goettingen, Germany
    Histochem Cell Biol 126:549-62. 2006
    ..However, a few Prox1(+)/CK19(+) and a few Prox1(+)/CK7(+ )cells were identified in the liver of AAF/PH-animals, which may represent precursors of hepatocytes, or a precancerous state...
  62. ncbi Liver initiation activity of norfloxacin but not nalidixic acid, pipemidic acid, and ciprofloxacin on in vivo short-term liver initiation assay in rats
    Tadashi Itoh
    Laboratory of Veterinary Pathology, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3 5 8 Saiwai cho, Fuchu, Tokyo 183 8509, Japan
    Toxicology 222:240-6. 2006
    ..These results suggest that only NFLX has an initiation activity in rats under the conditions used in the present study...
  63. ncbi Effects of aspirin on the in vitro and in vivo acetylation of 2-aminofluorene in Sprague-Dawley rats
    Song Shei Lin
    Department of Radiological Technology, Chungtai Institute of Health Sciences and Technology, Taichung, Taiwan, ROC
    In Vivo 19:475-81. 2005
    ..This is the first demonstration of acetylsalicylic acid (Aspirin) inhibition of arylamine N-acetyltransferase activity showing decreases in the N-acetylation of carcinogens in vivo...
  64. ncbi Altered expression of cytochrome P450 and possible correlation with preneoplastic changes in early stage of rat hepatocarcinogenesis
    Lin Lin Liu
    State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China
    Acta Pharmacol Sin 26:737-44. 2005
    ..To detect the expression of carcinogen-metabolizing related microsomal P450 enzymes, namely the CYP1A1, CYP1A2, CYP2B1/2, CYP2E1, and CYP3A, we performed the medium-term bioassay of Ito's model in Sprague-Dawley rats...
  65. ncbi Early induction of TGF-beta1 through a fasting-re-feeding regimen promotes liver carcinogenesis by a sub-initiating dose of diethylnitrosamine
    L Tessitore
    Department of Chemical, Nutritional, Pharmaceutical and Pharmacological Sciences, University A Avogadro, East Piedmont, Novara, Italy
    Cell Prolif 39:105-16. 2006
    ..Avoiding fasting-re-feeding could be considered in the nutritional status of patients with liver fibrosis...
  66. pmc Oncostatin M inhibits proliferation of rat oval cells, OC15-5, inducing differentiation into hepatocytes
    Atsuhito Okaya
    Department of Pathology, Hyogo College of Medicine, 1 1, Mukogawa, Nishinomiya, Hyogo 663 8501, Japan
    Am J Pathol 166:709-19. 2005
    ....
  67. ncbi Genetic and epigenetic changes in rat preneoplastic liver tissue induced by 2-acetylaminofluorene
    Tetyana V Bagnyukova
    Division of Biochemical Toxicology, National Center for Toxicological Research, Jefferson, AR 72079, USA
    Carcinogenesis 29:638-46. 2008
    ..In conclusion, we have demonstrated that exposure of rats to genotoxic hepatocarcinogen 2-AAF, in addition to formation of 2-AAF-specific DNA lesions, resulted in substantial alterations in cellular epigenetic status...
  68. ncbi Anti-tumor effect of pegylated interferon in the rat hepatocarcinogenesis model
    Akira Miki
    Division of Molecular Medicine and Medical Genetics, International Center of Medical Research and Treatment, Kobe University Graduate School of Medicine, Kusunoki cho, Kobe 650 0017, Japan
    Int J Oncol 32:603-8. 2008
    ..The inhibitory effect of the PEG-IFN and IFN on hepatocarcinogenesis was almost the same, and this might be induced by the Fas-related apoptosis in the surrounding tissues...
  69. ncbi Gene expression dose-response of liver with a genotoxic and nongenotoxic carcinogen
    Shawn D Seidel
    Toxicology and Environmental Research and Consulting, The Dow Chemical Company, Midland, Michigan 48674, USA
    Int J Toxicol 25:57-64. 2006
    ..These results suggest that the transcriptional response of the cells to higher doses of a toxic agent is likely to be different from that of a low-dose exposure...
  70. ncbi Activation of distinct multidrug-resistance (P-glycoprotein) genes during rat liver regeneration and hepatocarcinogenesis
    L D Teeter
    Department of Molecular Pathology, University of Texas M D Anderson Cancer Center, Houston 77030
    Mol Carcinog 8:67-73. 1993
    ..These results reveal a complex hepatic gene expression pattern during hepatocarcinogenesis and hepatic proliferation for this conserved gene family in rodents...
  71. ncbi Vanadium inhibits the development of 2-acetylaminofluorene-induced premalignant phenotype in a two-stage chemical rat hepatocarcinogenesis model
    Tridib Chakraborty
    Division of Biochemistry, Department of Pharmaceutical Technology, Jadavpur University, PO Box 17028, Calcutta 700032, West Bengal, India
    Life Sci 78:2839-51. 2006
    ..We conclude the significant role of vanadium in limiting cell proliferation and chromosomal aberrations during the preneoplastic stages of hepatocarcinogenesis in rats...
  72. ncbi A potential role of somatostatin and its receptor SSTR4 in the migration of hepatic oval cells
    Youngmi Jung
    Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL 32610 0275, USA
    Lab Invest 86:477-89. 2006
    ..These results suggest a positive role for SST in the migration of HOCs, and that this effect is mediated through SSTR4...
  73. ncbi Effects of oral administration of berberine on distribution and metabolism of 2-aminofluorene in Sprague-Dawley rats
    Chin Chung Lin
    Fong Yuan Hospital, Fong Yuan City 420, Taiwan
    In Vivo 21:321-8. 2007
    ..However, berberine pre-treatment followed by 2-AF led to different levels of 2-AF and 2-AAF, but not of 3-OH-2-AAF...
  74. ncbi Metabolic activation of N-hydroxy-2-aminofluorene and N-hydroxy-2-acetylaminofluorene by monomorphic N-acetyltransferase (NAT1) and polymorphic N-acetyltransferase (NAT2) in colon cytosols of Syrian hamsters congenic at the NAT2 locus
    D W Hein
    Department of Pharmacology and Toxicology, University of North Dakota School of Medicine, Grand Forks 58203
    Cancer Res 53:509-14. 1993
    ....
  75. ncbi Carcinogen induced unscheduled DNA synthesis in mouse hepatocytes
    J E Klaunig
    Department of Pathology, Medical College of Ohio, Toledo 43699, USA
    Toxicol Pathol 12:119-25. 1984
    ....
  76. ncbi Performance of 181 chemicals in a Drosophila assay predominantly monitoring interchromosomal mitotic recombination
    E W Vogel
    Department of Radiation Genetics and Chemical Mutagenesis, Leiden, Netherlands
    Mutagenesis 8:57-81. 1993
    ..Their weak responses were always accompanied by signs of toxicity.(ABSTRACT TRUNCATED AT 400 WORDS)..
  77. ncbi Evaluation of a multi-endpoint assay in rats, combining the bone-marrow micronucleus test, the Comet assay and the flow-cytometric peripheral blood micronucleus test
    Damian E Bowen
    JSC International Ltd, Simpson House, Windsor Court, Clarence Drive, Harrogate HG1 2PE, UK
    Mutat Res 722:7-19. 2011
    ..The data generated from these investigations demonstrate the suitability of the multi-endpoint design...
  78. ncbi Activation of the carcinogens N-hydroxy-N-2-fluorenylbenzamide and N-hydroxy-N-2-fluorenylacetamide via deacylations and acetyl transfers by rat peritoneal serosa and liver
    C L Ritter
    Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis 55455
    Carcinogenesis 15:163-70. 1994
    ..The study provides first evidence for activation of N-OH-2-FBA by rat liver and of both compounds by peritoneum in vitro...
  79. ncbi Induction of a heat shock response (HSP 72) in rat embryos exposed to selected chemical teratogens
    P E Mirkes
    Department of Pediatrics, University of Washington School of Medicine, Seattle 98195
    Teratology 49:135-42. 1994
    ..Although only a small sample of chemical teratogens was studied, our results suggest that the heat shock response, characterized by the synthesis and accumulation of HSP 72, is not a general biomarker for chemical teratogens...
  80. ncbi Expression of stem cell factor and its receptor, c-kit, during liver regeneration from putative stem cells in adult rat
    K Fujio
    Laboratory of Experimental Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, Maryland
    Lab Invest 70:511-6. 1994
    ..The SCF/c-kit system may, possibly in combination with other growth factor/receptor systems, be involved in the early activation of the hepatic stem cells as well as in the expansion and differentiation of oval cells...
  81. ncbi Ability of structurally related polycyclic aromatic carcinogens to induce homologous recombination between duplicated chromosomal sequences in mouse L cells
    N P Bhattacharyya
    Department of Microbiology, Michigan State University, East Lansing 48824 1316
    Mutat Res 211:205-14. 1989
    ..In the rest, only a single copy of the Htk gene remained, reflecting a single reciprocal exchange within a chromatid or a single unequal exchange between sister chromatids...
  82. ncbi Functional characterization of the rat mdr1b encoded P-glycoprotein: not all inducing agents are substrates
    E Santoni-Rugiu
    Division of Basic Sciences, National Cancer Institute, Bethesda, MD 20892, USA
    Carcinogenesis 18:2255-63. 1997
    ..The fact that compounds which are not themselves substrates can induce P-glycoprotein expression may have implications for pharmacokinetic interactions and chemotherapy...
  83. ncbi Ex vivo modulation of chemical-induced mutagenesis by subcellular liver fractions of rats treated with rooibos (Aspalathus linearis) tea, honeybush (Cyclopia intermedia) tea, as well as green and black (Camellia sinensis) teas
    J L Marnewick
    PROMEC Unit, Medical Research Council, P O Box 19070, Tygerberg 7505, South Africa
    Mutat Res 558:145-54. 2004
    ..05) enhanced the mutagenic response of 2-AAF. None of the tea treatments significantly affected the concentration of the microsomal liver cytochrome P450...
  84. ncbi Role of DNA polymerase 3'----5' exonuclease activity in the bypass of aminofluorene lesions in DNA
    B S Strauss
    Department of Molecular Genetics and Cell Biology, University of Chicago, IL 60637
    Carcinogenesis 11:2103-9. 1990
    ....
  85. ncbi Sulfation of carcinogenic aromatic hydroxylamines and hydroxamic acids by rat and human sulfotransferases: substrate specificity, developmental aspects and sex differences
    J H Meerman
    LACDR Center for Bio Pharmaceutical Sciences, Division of Toxicology, Leiden University, The Netherlands
    Chem Biol Interact 92:321-8. 1994
    ..Sulfation activity towards these compounds was also found in fetal and neonatal liver and adrenals. These compounds probably are sulfated by several different sulfotransferases in humans...
  86. pmc Different physiology of interferon-α/-γ in models of liver regeneration in the rat
    Danko S Batusic
    Department of Gastroenterology and Endocrinology, University of Gottingen, Gottingen, Germany
    Histochem Cell Biol 136:131-44. 2011
    ..Instead, inflammatory MNP infiltrating AAF/PH-treated livers produce IFN-α. These inflammatory MNPs may be involved in the regulation of the oval cell compartment through local expression of cytokines, including IFN-α...
  87. ncbi In situ detection of acetylaminofluorene-DNA adducts in human cells using monoclonal antibodies
    Taka aki Iwamoto
    Radioisotope Research Center, Department of Dermatology, Nara Medical University, Kashihara, Nara 634 8521, Japan
    DNA Repair (Amst) 3:1475-82. 2004
    ..Thus, we succeeded in establishing novel monoclonal antibodies capable of the in situ detection of NA-AAF-induced DNA adducts in human cells...
  88. ncbi Comparison of basal gene expression profiles and effects of hepatocarcinogens on gene expression in cultured primary human hepatocytes and HepG2 cells
    Angela J Harris
    Center for Hepatotoxicity, National Center for Toxicological Research, Food and Drug Administration, 3900 NCTR Drive, Jefferson, AR 72079, USA
    Mutat Res 549:79-99. 2004
    ..This study suggests that identification of biomarkers of exposure to some chemicals may be possible in the human through microarray analysis, despite the variability in responses...
  89. ncbi Tumor promoting and co-carcinogenic effects in medium-term rat hepatocarcinogenesis are not modified by co-administration of 12 pesticides in mixture at acceptable daily intake
    Julio Isael Pérez-Carreón
    UMR 1089 Xenobiotiques, Institut INRA ENVT, 180 chemin de Tournefeuille, BP 3, F 31 300 Toulouse, France
    Food Chem Toxicol 47:540-6. 2009
    ..Altogether these data contribute to the confidence that the ADI represents a safe intake level to mixture of pesticides at dietary exposure...
  90. ncbi Lack of p53 protein expression in preneoplastic rat hepatocytes in vitro after exposure to N-acetoxy-acetylaminofluorene, X-rays or a proteasome inhibitor
    H E Van Gijssel
    Division of Toxicology, Leiden Amsterdam Center for Drug Research, Leiden University, The Netherlands
    Eur J Cancer 36:106-12. 2000
    ..Therefore, loss of p53 may favour clonal expansion of preneoplastic hepatocytes in the rat after administration of hepatocarcinogens or X-rays...
  91. ncbi Translesion synthesis in Escherichia coli: lessons from the NarI mutation hot spot
    Robert P Fuchs
    Genome Instability and Carcinogenesis, CNRS FRE 2931, 31 Chemin Joseph Aiguier, Marseille, France
    DNA Repair (Amst) 6:1032-41. 2007
    ..The beta-clamp, to which all DNA polymerases bind, plays a critical role in allowing the specialized DNA polymerases to synthesize TLS patches that are long enough to resist such "external proofreading" activities...
  92. ncbi Homodimeric and heterodimeric aryl sulfotransferases catalyze the sulfuric acid esterification of N-hydroxy-2-acetylaminofluorene
    C C Kiehlbauch
    Oklahoma Medical Research Foundation, Noble Center for Biomedical Research, Oklahoma City 73104 5046, USA
    J Biol Chem 270:18941-7. 1995
    ....
  93. pmc The sensitivity of human fibroblasts to N-acetoxy-2-acetylaminofluorene is determined by the extent of transcription-coupled repair, and/or their capability to counteract RNA synthesis inhibition
    M F van Oosterwijk
    MGC Department of Radiation Genetics and Chemical Mutagenesis, Leiden University, The Netherlands
    Nucleic Acids Res 24:4653-9. 1996
    ..This indicates that the subset of lesions which are capable of stalling RNA polymerase II, and are, therefore, a substrate for TCR, are also the lesions which are very efficiently recognized by the global genome repair system...
  94. ncbi Induction of DNA strand breaks by genotoxicants in the alga Chlamydomonas reinhardtii
    Rhiannon M David
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom
    Environ Toxicol Chem 28:1893-900. 2009
    ..In conclusion, C reinhardtii responded, albeit weakly, to selected direct- and indirect-acting genotoxicants and also exhibited measurable EROD activity...
  95. ncbi Alternatives in the induction and preparation of phenobarbital/naphthoflavone-induced S9 and their activation profiles
    S Garcia Franco
    Instituto de Farmacología y Toxicología Roemmers, Buenos Aires, Argentina
    Mutagenesis 14:323-6. 1999
    ..These results suggest that the simultaneous treatment (NaPB + betaNF) would be an adequate inducer for in vitro activation when used at 700-1000 microg protein/plate...
  96. ncbi Comparing the frequency and spectra of mutations induced when an SV-40 based shuttle vector containing covalently bound residues of structurally-related carcinogens replicates in human cells
    V M Maher
    Department of Microbiology, Michigan State University, East Lansing 48824 1316
    Mutat Res 220:83-92. 1989
    ..abstract truncated at 400 words)..
  97. ncbi Cytochrome P4501A2 constitutively expressed from transduced DNA mediates metabolic activation and DNA-adduct formation of aromatic amine carcinogens in NIH 3T3 cells
    N Battula
    Laboratory of Experimental Carcinogenesis, National Cancer Institute, Bethesda, Maryland
    Mol Carcinog 4:407-14. 1991
    ....
  98. ncbi Steroidal nonspecific esterase metabolism of N-hydroxy-2-acetylaminofluorene: evidence for selective activation by the cellular reductant NADPH
    M Lund-Pero
    Preventive Medicine Institute, Strang Clinic, Division of Biochemical Epidemiology, New York, New York
    J Leukoc Biol 51:133-9. 1992
    ..Instead, they support the direct and specific influence of NADPH as a widespread activator of esterase activity by a mechanism not yet understood...
  99. ncbi Dietary lycopene decreases the initiation of liver preneoplastic foci by diethylnitrosamine in the rat
    P Astorg
    Unite de Toxicologie Nutritionnelle, Institut National de la Recherche Agronomique, Dijon, France
    Nutr Cancer 29:60-8. 1997
    ..Lycopene does not appear to act through its antioxidant properties, but rather through its modulating effect on the liver enzyme activating DEN, cytochrome P-450 2E1...
  100. pmc Role of Nrf2 in the regulation of the Mrp2 (ABCC2) gene
    Valeska Vollrath
    Department of Gastroenterology, School of Medicine, Catholic University of Chile, Casilla 114 D, Santiago, Chile
    Biochem J 395:599-609. 2006
    ..Thus Nrf2 appears to regulate Mrp2 gene expression via an ARE element located at the proximal region of its promoter in response to exposure to xenobiotics...
  101. ncbi Evaluation of a liver micronucleus assay with 12 chemicals using young rats (II): a study by the Collaborative Study Group for the Micronucleus Test/Japanese Environmental Mutagen Society-Mammalian Mutagenicity Study Group
    Hiroshi Suzuki
    Ina Research Inc, Ina shi, Nagano, Japan
    Mutagenesis 24:9-16. 2009
    ..Based on these results, it is concluded that this technique could be effective for detecting chemical clastogenicity in hepatocytes in vivo...

Research Grants36

  1. Mechanisms of oval cell activation and differentiation
    Bryon E Petersen; Fiscal Year: 2013
    ..This knowledge will help to guide the development of strategies for the therapeutic use of stem cell transplants for the treatment of liver disease. ..
  2. Gender Disparity in Bladder Cancer and Chemopreventive Intervention
    Yuesheng Zhang; Fiscal Year: 2013
    ..Impact: The proposed studies are expected to lead to the elucidation of the molecular basis of the gender disparity in BC and the development of new strategy for effective prevention of this disease. ..
  3. HEPATOBILIARY DISPOSITION IN TOXICOLOGY
    Curtis Klaassen; Fiscal Year: 2003
    ..abstract_text> ..
  4. STRUCTURE AND FUNCTION OF EUKARYOTIC CHROMOSOMES
    Barbara Hamkalo; Fiscal Year: 1990
    ....
  5. HUMAN MISMATCH REPAIR IN CHEMICAL CARCINOGENESIS
    Guo Min Li; Fiscal Year: 2001
    ....
  6. METABOLISM OF ACETYLAMINOFLOURENE BY RAINBOW TROUT
    HARISH SIKKA; Fiscal Year: 1993
    ....
  7. INHIBITION OF CARCINOGENESIS BY SELENIUM
    Arthur Cohen; Fiscal Year: 1980
    ..In conjunction with the studies described above, these investigations should provide new insights into the ability of selenium to protect the liver against potential changes in gene function induced by FAA. ..
  8. METABOLISM OF ENVIRONMENTAL CONTAMINANTS BY FISH
    JOHN LECH; Fiscal Year: 1992
    ..The chemicals to be studied include chlordimeform, fenitrothion, aldicarb and N- acetylaminofluorene...
  9. CARCINOGENESIS & CELL EXTRACTS REACTION WITH RF-DNA
    William Taylor; Fiscal Year: 1980
    ....
  10. INITIATION--PROMOTION BIOASSAY IN MOUSE LIVER
    Michael Pereira; Fiscal Year: 1990
    ....
  11. CARCINOGEN DAMAGE AND REPAIR OF DNA IN HUMAN CHROMATIN
    Michael Smerdon; Fiscal Year: 1980
    ....
  12. REPAIR SYNTHESIS OF DNA IN PRECANCEROUS RAT LIVER
    Jay Goodman; Fiscal Year: 1980
    ..Novikoff hepatoma cells grown in suspension culture will be employed for studies on the fidelity of the DNA repair process, as assessed by changes in mutation frequency. ..
  13. Flow cytometry-based Unscheduled DNA Synthesis (FLUDS)
    GEORGE DEGEORGE; Fiscal Year: 2005
    ..The pre-validated FLUDS assay would be of considerable commercial value to customers in the pharmaceutical, chemical, cosmetic and consumer products industries. ..
  14. ERROR-PRONE DNA REPAIR IN HEPATOCARCINOGENESIS
    JAMES YAGER; Fiscal Year: 1980
    ....
  15. DNA REPAIR AND CANCER PRONE HEREDITARY HUMAN DISEASE
    Errol Friedberg; Fiscal Year: 2002
    ....
  16. REGULATION OF MDR GENE EXPRESSION IN LIVER CANCERS
    MACUS KUO; Fiscal Year: 2004
    ..We hope from this research, which includes in vitro cell culture and in vivo animal and tumor models, to gain important molecular insights into the genetic resolution of drug resistance and hepatocarcinogenesis. ..
  17. Colon and liver anticarcinogenicity by acetaminophen
    Gary Williams; Fiscal Year: 2004
    ..In addition, these experiments will provide support for the epidemiological observations of reduced cancer risk associated with the use of APAP. ..
  18. Versatile Mutation Assay Platform
    Stephen Dertinger; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  19. High Throughput Radiation Biodosimetry
    Stephen Dertinger; Fiscal Year: 2007
    ..We propose to develop such a radiation biodosimetry assay based on the extremely high analysis rates that are possible with flow cytometry instrumentation. [unreadable] [unreadable] [unreadable]..
  20. Methodological Research on Substance Use
    Heping Zhang; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  21. Rapid Screen for Genotoxicants, Chemoprotectors, and Radioprotectors
    Stephen Dertinger; Fiscal Year: 2008
    ..In addition, this assay can be used to investigate agents designed to ameliorate the effects of radiation exposure, e.g. biological response modifiers. [unreadable] [unreadable] [unreadable] [unreadable]..
  22. Sequence Effects of Arylamine-DNA Adducts: Repair and Replication
    BONGSUP CHO; Fiscal Year: 2008
    ..Such knowledge will also be of help in the development of sensible prevention and risk assessment strategies. ..
  23. Insulin producing cells generated in vitro from hepatic stem cells
    Lijun Yang; Fiscal Year: 2007
    ..abstract_text> ..
  24. Genotoxicity of Estrogen-and Anti-estrogen-DNA Adducts
    Shinya Shibutani; Fiscal Year: 2006
    ..This research should lead to a safer alternative for women undergoing breast cancer therapy and for chemoprevention. ..
  25. Autoimmunity of hepatocyte-derived insulin-producing*
    Lijun Yang; Fiscal Year: 2005
    ..The information derived from these experiments is critical for translating the results of liver to endocrine pancreas transdifferentiation into clinical application for gene and cell based therapies aimed at curing TIDM. ..
  26. PATHWAYS TO ALCOHOLISM THROUGH PSYCHOPATHOLOGY
    Heping Zhang; Fiscal Year: 2001
    ..They further state that identifying these pathways will ultimately assist in the development of an empirical basis for the primary prevention of secondary alcoholism. ..
  27. Measuring Cytogenetic Damage in Human Blood
    Stephen Dertinger; Fiscal Year: 2003
    ..abstract_text> ..
  28. Automation of the vitro Micronucleus Assay
    Stephen Dertinger; Fiscal Year: 2004
    ..abstract_text> ..
  29. In vivo mutation assay based on pig-a locus
    Stephen Dertinger; Fiscal Year: 2004
    ....
  30. Transdifferentiation of hepatocytes
    Lijun Yang; Fiscal Year: 2005
    ..abstract_text> ..
  31. STATISTICAL METHODS FOR CORRELATED SUBSTANCE USE DATA
    Heping Zhang; Fiscal Year: 2003
    ....
  32. RESEARCH TRAINING IN THE EPIDEMIOLOGY OF AGING
    Stanislav Kasl; Fiscal Year: 2008
    ..In addition, Ph.D. candidates in biostatistics who will work with our biostatistics faculty on developing new techniques for the analysis of longitudinal data on older persons will also be eligible for support. ..
  33. Liver to Endocrine Pancreas Transdifferentiation
    Lijun Yang; Fiscal Year: 2009
    ..abstract_text> ..
  34. Genotoxicity of Estrogen Compounds in Endocrine Therapy
    Shinya Shibutani; Fiscal Year: 2009
    ..This research will also provide biomarkers that can be used to identify subgroups of women at high risk of developing ERT-induced cancer. ..