Summary: A highly reactive aldehyde gas formed by oxidation or incomplete combustion of hydrocarbons. In solution, it has a wide range of uses: in the manufacture of resins and textiles, as a disinfectant, and as a laboratory fixative or preservative. Formaldehyde solution (formalin) is considered a hazardous compound, and its vapor toxic. (From Reynolds, Martindale The Extra Pharmacopoeia, 30th ed, p717)

Top Publications

  1. pmc FAIRE (Formaldehyde-Assisted Isolation of Regulatory Elements) isolates active regulatory elements from human chromatin
    Paul G Giresi
    Department of Biology and the Carolina Center for Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3280, USA
    Genome Res 17:877-85. 2007
  2. ncbi Nociceptive-specific activation of ERK in spinal neurons contributes to pain hypersensitivity
    R R Ji
    Neural Plasticity Research Group, Department of Anesthesia and Critical Care, Massachusetts General Hospital and Harvard Medical School, 149 13th Street, Rm 4309, Charlestown, Massachusetts 02129, USA
    Nat Neurosci 2:1114-9. 1999
  3. pmc TRPA1 mediates formalin-induced pain
    Colleen R McNamara
    Hydra Biosciences, Inc, 790 Memorial Drive, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 104:13525-30. 2007
  4. pmc Using formaldehyde-assisted isolation of regulatory elements (FAIRE) to isolate active regulatory DNA
    Jeremy M Simon
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    Nat Protoc 7:256-67. 2012
  5. ncbi RNA expression analysis from formalin fixed paraffin embedded tissues
    Susan M Farragher
    Almac Diagnostics, Craigavon, 19 Seagoe Industrial Estate, Armagh BT635QD, UK
    Histochem Cell Biol 130:435-45. 2008
  6. pmc Isolation of active regulatory elements from eukaryotic chromatin using FAIRE (Formaldehyde Assisted Isolation of Regulatory Elements)
    Paul G Giresi
    Department of Biology and Carolina Center for the Genome Sciences, University of North Carolina at Chapel Hill, CB 3280, 408 Fordham Hall, Chapel Hill, NC 27599 3280, USA
    Methods 48:233-9. 2009
  7. pmc Whole-genome gene expression profiling of formalin-fixed, paraffin-embedded tissue samples
    Craig April
    Illumina, Inc, San Diego, California, United States of America
    PLoS ONE 4:e8162. 2009
  8. ncbi Histone demethylation mediated by the nuclear amine oxidase homolog LSD1
    Yujiang Shi
    Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Cell 119:941-53. 2004
  9. ncbi AlkB-mediated oxidative demethylation reverses DNA damage in Escherichia coli
    Pål Ø Falnes
    Centre for Molecular Biology and Neuroscience, and Institute of Medical Microbiology, University of Oslo, National Hospital, 0027 Oslo, Norway
    Nature 419:178-82. 2002
  10. pmc Formaldehyde-based whole-mount in situ hybridization method for planarians
    Bret J Pearson
    Department of Neurobiology and Anatomy, Howard Hughes Medical Institute, University of Utah School of Medicine, Salt Lake City, Utah, USA
    Dev Dyn 238:443-50. 2009

Detail Information

Publications323 found, 100 shown here

  1. pmc FAIRE (Formaldehyde-Assisted Isolation of Regulatory Elements) isolates active regulatory elements from human chromatin
    Paul G Giresi
    Department of Biology and the Carolina Center for Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3280, USA
    Genome Res 17:877-85. 2007
    ..demonstrate a simple procedure for the isolation of nucleosome-depleted DNA from human chromatin, termed FAIRE (Formaldehyde-Assisted Isolation of Regulatory Elements)...
  2. ncbi Nociceptive-specific activation of ERK in spinal neurons contributes to pain hypersensitivity
    R R Ji
    Neural Plasticity Research Group, Department of Anesthesia and Critical Care, Massachusetts General Hospital and Harvard Medical School, 149 13th Street, Rm 4309, Charlestown, Massachusetts 02129, USA
    Nat Neurosci 2:1114-9. 1999
    ..ERK signaling within the spinal cord is therefore involved in generating pain hypersensitivity. Because of its rapid activation, this effect probably involves regulation of neuronal excitability without changes in transcription...
  3. pmc TRPA1 mediates formalin-induced pain
    Colleen R McNamara
    Hydra Biosciences, Inc, 790 Memorial Drive, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 104:13525-30. 2007
  4. pmc Using formaldehyde-assisted isolation of regulatory elements (FAIRE) to isolate active regulatory DNA
    Jeremy M Simon
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    Nat Protoc 7:256-67. 2012
    ..FAIRE (formaldehyde-assisted isolation of regulatory elements) is an alternative approach to identify these genomic regions and has ..
  5. ncbi RNA expression analysis from formalin fixed paraffin embedded tissues
    Susan M Farragher
    Almac Diagnostics, Craigavon, 19 Seagoe Industrial Estate, Armagh BT635QD, UK
    Histochem Cell Biol 130:435-45. 2008
    ..This paper reviews the progress in this field and discusses the unique opportunities that exist for the application of these techniques in the development of personalized medicine...
  6. pmc Isolation of active regulatory elements from eukaryotic chromatin using FAIRE (Formaldehyde Assisted Isolation of Regulatory Elements)
    Paul G Giresi
    Department of Biology and Carolina Center for the Genome Sciences, University of North Carolina at Chapel Hill, CB 3280, 408 Fordham Hall, Chapel Hill, NC 27599 3280, USA
    Methods 48:233-9. 2009
    ..a simple procedure for the genome-wide isolation of nucleosome-depleted DNA from human chromatin, termed FAIRE (Formaldehyde Assisted Isolation of Regulatory Elements)...
  7. pmc Whole-genome gene expression profiling of formalin-fixed, paraffin-embedded tissue samples
    Craig April
    Illumina, Inc, San Diego, California, United States of America
    PLoS ONE 4:e8162. 2009
    ..We have developed a gene expression assay (Whole-Genome DASL), capable of generating whole-genome gene expression profiles from degraded samples such as formalin-fixed, paraffin-embedded (FFPE) specimens...
  8. ncbi Histone demethylation mediated by the nuclear amine oxidase homolog LSD1
    Yujiang Shi
    Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Cell 119:941-53. 2004
    ..Lysine demethylation occurs via an oxidation reaction that generates formaldehyde. Importantly, RNAi inhibition of LSD1 causes an increase in H3 lysine 4 methylation and concomitant ..
  9. ncbi AlkB-mediated oxidative demethylation reverses DNA damage in Escherichia coli
    Pål Ø Falnes
    Centre for Molecular Biology and Neuroscience, and Institute of Medical Microbiology, University of Oslo, National Hospital, 0027 Oslo, Norway
    Nature 419:178-82. 2002
    ..This mechanism represents a new pathway for DNA repair and the third type of DNA damage reversal mechanism so far discovered...
  10. pmc Formaldehyde-based whole-mount in situ hybridization method for planarians
    Bret J Pearson
    Department of Neurobiology and Anatomy, Howard Hughes Medical Institute, University of Utah School of Medicine, Salt Lake City, Utah, USA
    Dev Dyn 238:443-50. 2009
    ..Here, we describe a formaldehyde-based WISH method for the freshwater planarian Schmidtea mediterranea developed by systematically comparing and ..
  11. pmc Targeted high throughput sequencing in clinical cancer settings: formaldehyde fixed-paraffin embedded (FFPE) tumor tissues, input amount and tumor heterogeneity
    Martin Kerick
    Max Planck Institute for Molecular Genetics, Ihnestr, Berlin, Germany
    BMC Med Genomics 4:68. 2011
    ..However, these technologies need to be further improved with regard to reproducibility and applicability to clinical samples and settings...
  12. ncbi Generation of high-quality protein extracts from formalin-fixed, paraffin-embedded tissues
    Maria Filippa Addis
    Porto Conte Ricerche Srl, Tramariglio, Alghero, Sassari, Italy
    Proteomics 9:3815-23. 2009
    ..An evaluation of the extent of modifications introduced on proteins by formalin fixation and crosslink reversal, and their impact on quality of MS results, is also reported...
  13. ncbi Urine formaldehyde level is inversely correlated to mini mental state examination scores in senile dementia
    Zhiqian Tong
    State Key Lab of Brain and Cognitive Sciences, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
    Neurobiol Aging 32:31-41. 2011
    It is widely known that exogenous formaldehyde exposure induces human cognitive impairment and animal memory loss; and recent studies show that formaldehyde at pathological levels induces Aβ deposition and misfolding of tau protein to ..
  14. pmc Determinants of RNA quality from FFPE samples
    Silke von Ahlfen
    QIAGEN GmbH, Hilden, Germany
    PLoS ONE 2:e1261. 2007
    ..The results are particularly relevant for any application relying on cDNA synthesis as an initial step of the procedure, such as RT-PCR, and microarray analysis...
  15. ncbi Effect of inhaled formaldehyde on learning and memory of mice
    Z Lu
    College of Life Science, Central China Normal University, Wuhan, China
    Indoor Air 18:77-83. 2008
    In this study, we investigated the effect of inhaled formaldehyde on learning and memory capacity...
  16. ncbi Activation of p38 mitogen-activated protein kinase in spinal microglia is a critical link in inflammation-induced spinal pain processing
    Camilla I Svensson
    Department of Anesthesiology, University of California, San Diego, La Jolla, California, USA
    J Neurochem 86:1534-44. 2003
    ..Taken together, these results indicate that spinal p38 MAPK is involved in inflammation-induced pain and that activated spinal microglia play a direct role in spinal nociceptive processing...
  17. pmc Is exposure to formaldehyde in air causally associated with leukemia?--A hypothesis-based weight-of-evidence analysis
    Lorenz R Rhomberg
    Gradient, Cambridge, Massachusetts 02138, USA
    Crit Rev Toxicol 41:555-621. 2011
    Recent scientific debate has focused on the potential for inhaled formaldehyde to cause lymphohematopoietic cancers, particularly leukemias, in humans...
  18. pmc Reproductive and developmental toxicity of formaldehyde: a systematic review
    Anh Duong
    School of Public Health, University of California, Berkeley, CA 94720, USA
    Mutat Res 728:118-38. 2011
    b>Formaldehyde, the recently classified carcinogen and ubiquitous environmental contaminant, has long been suspected of causing adverse reproductive and developmental effects, but previous reviews were inconclusive, due in part, to ..
  19. pmc Formaldehyde-inactivated whole-virus vaccine protects a murine model of enterovirus 71 encephalomyelitis against disease
    Kien Chai Ong
    Department of Pathology, University of Malaya, 50603 Kuala Lumpur, Malaysia
    J Virol 84:661-5. 2010
    ..b>Formaldehyde-inactivated whole-virus vaccines derived from EV71 clinical isolates and a mouse-adapted virus (MAV) were ..
  20. pmc miRNA in situ hybridization in formaldehyde and EDC-fixed tissues
    John T G Pena
    Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, New York 10065, USA
    Nat Methods 6:139-41. 2009
    ..We showed that in situ hybridization (ISH) using conventional formaldehyde fixation results in substantial microRNA loss from mouse tissue sections, which can be prevented by fixation ..
  21. pmc Gene expression profiles from formalin fixed paraffin embedded breast cancer tissue are largely comparable to fresh frozen matched tissue
    Lorenza Mittempergher
    Divisions of Experimental Therapy, Molecular Biology, Surgical Oncology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    PLoS ONE 6:e17163. 2011
    ..To this end we profiled, in duplicate, 20 FFPE tissues and 20 matched FF tissues and evaluated the concordance of the DASL results from FFPE and matched FF material...
  22. pmc Limited copy number-high resolution melting (LCN-HRM) enables the detection and identification by sequencing of low level mutations in cancer biopsies
    Hongdo Do
    Department of Pathology, University of Melbourne, Parkville, Victoria, 3010, Australia
    Mol Cancer 8:82. 2009
    ..The dilutions used (based on Ct values) are chosen such that mutations, if present, can be detected by the direct sequencing of amplicons with aberrant melting patterns...
  23. ncbi Domestic exposure to formaldehyde significantly increases the risk of asthma in young children
    K B Rumchev
    Eur Respir J 20:403-8. 2002
    Concern has arisen in recent years about indoor air pollution as a risk factor for asthma. Formaldehyde exposure was examined in relation to asthma among young children (between 6 months and 3 yrs old) in a population-based control study ..
  24. pmc Optimization of formaldehyde cross-linking for protein interaction analysis of non-tagged integrin beta1
    Cordula Klockenbusch
    The Biomedical Research Centre, University of British Columbia, Vancouver, BC V6T1Z3, Canada
    J Biomed Biotechnol 2010:927585. 2010
    b>Formaldehyde cross-linking of protein complexes combined with immunoprecipitation and mass spectrometry analysis is a promising technique for analysing protein-protein interactions, including those of transient nature...
  25. ncbi Identification of formaldehyde-induced modifications in proteins: reactions with model peptides
    Bernard Metz
    Unit Research and Development, The Netherlands Vaccine Institute, 3720 AL Bilthoven, The Netherlands
    J Biol Chem 279:6235-43. 2004
    b>Formaldehyde is a well known cross-linking agent that can inactivate, stabilize, or immobilize proteins. The purpose of this study was to map the chemical modifications occurring on each natural amino acid residue caused by formaldehyde...
  26. pmc Epigenetic changes induced by air toxics: formaldehyde exposure alters miRNA expression profiles in human lung cells
    Julia E Rager
    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Environ Health Perspect 119:494-500. 2011
    Exposure to formaldehyde, a known air toxic, is associated with cancer and lung disease. Despite the adverse health effects of formaldehyde, the mechanisms underlying formaldehyde-induced disease remain largely unknown...
  27. pmc Systematic analysis of microRNA expression of RNA extracted from fresh frozen and formalin-fixed paraffin-embedded samples
    Yaguang Xi
    Cancer Genomics Laboratory, Mitchell Cancer Institute, Mobile, Alabama 36688, USA
    RNA 13:1668-74. 2007
    ..There are no significant differences of representative miRNA expression among 40 colorectal cancer FFPE specimens. This study provides a foundation for miRNA investigation using FFPE samples in cancer and other types of diseases...
  28. ncbi Induction of endoplasmic reticulum stress and the modulation of thioredoxin-1 in formaldehyde-induced neurotoxicity
    Fu Cheng Luo
    Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China
    Neurotoxicology 33:290-8. 2012
    b>Formaldehyde (FA), a common environmental pollutant, has toxic effects on central nervous system. The detailed mechanisms on FA-induced neurotoxicity have not been fully elucidated...
  29. pmc Formaldehyde exposure and asthma in children: a systematic review
    Gerald McGwin
    Department of Epidemiology, School of Public Health, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Environ Health Perspect 118:313-7. 2010
    Despite multiple published studies regarding the association between formaldehyde exposure and childhood asthma, a consistent association has not been identified...
  30. pmc Validation and reproducibility of a microarray-based gene expression test for tumor identification in formalin-fixed, paraffin-embedded specimens
    Raji Pillai
    Pathwork Diagnostics, Inc, Redwood City, California 94063 4737, USA
    J Mol Diagn 13:48-56. 2011
    ..3% concordance between laboratories. The Tissue of Origin Test makes the benefits of microarray-based gene expression tests for tumor diagnosis available for use with the most common type of histology specimen (ie, FFPE)...
  31. pmc Tumor tissue-derived formaldehyde and acidic microenvironment synergistically induce bone cancer pain
    Zhiqian Tong
    Neuroscience Research Institute, Peking University, Beijing, China
    PLoS ONE 5:e10234. 2010
    ..Accumulated evidence shows that endogenous formaldehyde concentrations are elevated in the blood or urine of patients with breast, prostate or bladder cancer...
  32. ncbi DNA extraction from archival formalin-fixed, paraffin-embedded tissue sections based on the antigen retrieval principle: heating under the influence of pH
    Shan Rong Shi
    Department of Pathology, University of Southern California Keck School of Medicine, Los Angeles, California 90033, USA
    J Histochem Cytochem 50:1005-11. 2002
    ..These findings may lead to a more effective protocol for DNA extraction from archival paraffin-embedded tissues and may also provide enhanced understanding of changes that occur during formalin-induced modification of nucleic acids...
  33. pmc A multiplex PCR predictor for aCGH success of FFPE samples
    E H van Beers
    Division of Experimental Therapy, Netherlands Cancer Institute NKI AvL, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Br J Cancer 94:333-7. 2006
  34. ncbi Evidence for the involvement of spinal cord glia in subcutaneous formalin induced hyperalgesia in the rat
    L R Watkins
    Department of Psychology, University of Colorado at Boulder, 80309, USA
    Pain 71:225-35. 1997
    ..Taken together, the present series of experiments suggest an important role for spinal glial cells in the cascade of events that are initiated by descending signals following s.c. administration of formalin...
  35. ncbi Quantitative protein analysis from formalin-fixed tissues: implications for translational clinical research and nanoscale molecular diagnosis
    K F Becker
    Technische Universitat, Institut fur Pathologie, Munchen, Germany
    J Pathol 211:370-8. 2007
    ..This technique may also be used to identify, characterize, and validate known and new protein markers in a variety of human diseases...
  36. pmc Genome-wide massively parallel sequencing of formaldehyde fixed-paraffin embedded (FFPE) tumor tissues for copy-number- and mutation-analysis
    Michal R Schweiger
    Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Berlin, Germany
    PLoS ONE 4:e5548. 2009
    ..However, FFPE preparation is incompatible with many down-stream molecular biology techniques such as PCR based amplification methods and gene expression studies...
  37. ncbi Proteomic analysis of formalin-fixed prostate cancer tissue
    Brian L Hood
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute, Frederick, MD 21702, USA
    Mol Cell Proteomics 4:1741-53. 2005
  38. ncbi Tissue handling and specimen preparation in surgical pathology: issues concerning the recovery of nucleic acids from formalin-fixed, paraffin-embedded tissue
    Stephen M Hewitt
    Tissue Array Research Program, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 4605, USA
    Arch Pathol Lab Med 132:1929-35. 2008
    ..High-quality nucleic acids can be obtained from formalin-fixed, paraffin-embedded tissue, but greater attention to all steps in the process of tissue handling and preparation is required...
  39. pmc A temporal threshold for formaldehyde crosslinking and fixation
    Lars Schmiedeberg
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, United Kingdom
    PLoS ONE 4:e4636. 2009
    b>Formaldehyde crosslinking is in widespread use as a biological fixative for microscopy and molecular biology...
  40. ncbi Real-time PCR analysis of DNA and RNA extracted from formalin-fixed and paraffin-embedded biopsies
    U Lehmann
    Institute of Pathology, Medizinische Hochschule Hannover, Carl Neuberg Strasse 1, D 30625 Hannover, Germany
    Methods 25:409-18. 2001
    ..We provide a number of protocols to assist in the application of these techniques...
  41. pmc Mortality from lymphohematopoietic malignancies and brain cancer among embalmers exposed to formaldehyde
    Michael Hauptmann
    Department of Bioinformatics and Statistics, Netherlands Cancer Institute, Amsterdam, The Netherlands
    J Natl Cancer Inst 101:1696-708. 2009
    ..found in surveys of anatomists, pathologists, and funeral industry workers, all of whom may have worked with formaldehyde. We investigated the relation of mortality to work practices and formaldehyde exposure levels among these ..
  42. ncbi Gene expression profiling of formalin-fixed, paraffin-embedded familial breast tumours using the whole genome-DASL assay
    Nic Waddell
    The Queensland Institute of Medical Research, Brisbane, Australia
    J Pathol 221:452-61. 2010
  43. pmc Equivalence of protein inventories obtained from formalin-fixed paraffin-embedded and frozen tissue in multidimensional liquid chromatography-tandem mass spectrometry shotgun proteomic analysis
    Robert W Sprung
    Jim Ayers Institute for Precancer Detection and Diagnosis, Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 8575, USA
    Mol Cell Proteomics 8:1988-98. 2009
    ..No covalent peptide modifications attributable to formaldehyde chemistry were detected by analyses of the MS/MS datasets, which suggests that undetected, cross-linked ..
  44. pmc Quantitative expression profiling in formalin-fixed paraffin-embedded samples by affymetrix microarrays
    Diana Abdueva
    Department of Pathology, Children s Hospital Los Angeles, Research Institute and Keck School of Medicine, University of Southern California, Los Angeles, California, USA
    J Mol Diagn 12:409-17. 2010
    ..This study provides guidelines for application of these methods in the discovery, validation, and clinical application of microarray expression profiling with FFPE material...
  45. pmc Optimization of RNA extraction from FFPE tissues for expression profiling in the DASL assay
    Mark Abramovitz
    VM Institute of Research, Montreal, Quebec, Canada
    Biotechniques 44:417-23. 2008
  46. ncbi Direct cancer tissue proteomics: a method to identify candidate cancer biomarkers from formalin-fixed paraffin-embedded archival tissues
    S I Hwang
    Department of Cell Biology, Center for Vascular Biology, University of Connecticut School of Medicine, Farmington, CT 06030, USA
    Oncogene 26:65-76. 2007
    ..We propose that this general strategy provides a roadmap for successful identification of critical molecular targets of multiple cancer types...
  47. pmc Multiple formaldehyde oxidation/detoxification pathways in Burkholderia fungorum LB400
    Christopher J Marx
    Department of Microbiology, University of Washington, Seattle, Washington 98195 2180, USA
    J Bacteriol 186:2173-8. 2004
    ..The genome of B. fungorum LB400 is predicted to encode three different pathways for formaldehyde oxidation: an NAD-linked, glutathione (GSH)-independent formaldehyde dehydrogenase; an NAD-linked, GSH-..
  48. ncbi Disturbances of soluble N-ethylmaleimide-sensitive factor attachment proteins in hippocampal synaptosomes contribute to cognitive impairment after repetitive formaldehyde inhalation in male rats
    Y Liu
    Neuroscience Research Institute and Department of Neurobiology, Key Laboratory for Neuroscience, Ministry of Education, Ministry of Public Health, Peking University Health Science Center, Beijing 100191, PR China
    Neuroscience 169:1248-54. 2010
    ..In the present study, we evaluated the effects of formaldehyde (FA) inhalation (13.5+/-1...
  49. ncbi Formaldehyde and chemosensory irritation in humans: a controlled human exposure study
    Isabelle Lang
    Institute and Out patient Clinic of Occupational and Social Medicine, University of Heidelberg, Vossstrasse 2, 69115 Heidelberg, Germany
    Regul Toxicol Pharmacol 50:23-36. 2008 examine the possible occurrence of sensory irritation and subjective symptoms in human volunteers exposed to formaldehyde concentrations relevant to the workplace...
  50. ncbi Pharmacological assessment of the rat formalin test utilizing the clinically used analgesic drugs gabapentin, lamotrigine, morphine, duloxetine, tramadol and ibuprofen: influence of low and high formalin concentrations
    Gordon Munro
    Department of Pharmacology, Neurosearch A S, Ballerup, Denmark
    Eur J Pharmacol 605:95-102. 2009
  51. ncbi Metabolic mechanisms of methanol/formaldehyde in isolated rat hepatocytes: carbonyl-metabolizing enzymes versus oxidative stress
    Stephanie L Macallister
    Department of Pharmaceutical Science, Faculty of Pharmacy, University of Toronto, Toronto, Ontario, Canada
    Chem Biol Interact 191:308-14. 2011
    ..Hepatocyte protein carbonylation induced by formaldehyde (HCHO) formation was also increased about 4-fold, when CH(3)OH was oxidized by the Fenton-like system, Fe(II)/H(..
  52. ncbi An in vitro approach to assess the toxicity of inhaled tobacco smoke components: nicotine, cadmium, formaldehyde and urethane
    Dominique Balharry
    Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, South Glamorgan, CF10 3US, UK
    Toxicology 244:66-76. 2008
    ..toxicants, the EpiAirway tissue model (ETM) was treated apically with tobacco smoke components (nicotine, formaldehyde, cadmium, urethane) which are known to induce a variety of toxic effects (e.g...
  53. ncbi Role of peripheral 5-HT(4), 5-HT(6), and 5-HT(7) receptors in development and maintenance of secondary mechanical allodynia and hyperalgesia
    Beatriz Godínez-Chaparro
    Departamento de Farmacobiologia, Centro de Investigación y de Estudios Avanzados Cinvestav, Sede Sur, Mexico DF, Mexico
    Pain 152:687-97. 2011
  54. pmc Treatment of inflammatory and neuropathic pain by uncoupling Src from the NMDA receptor complex
    Xue Jun Liu
    Program in Neurosciences and Mental Health, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada
    Nat Med 14:1325-32. 2008
  55. ncbi The antidepressant phenelzine protects neurons and astrocytes against formaldehyde-induced toxicity
    Mee Sook Song
    Neurochemical Research Unit, Department of Psychiatry, University of Alberta, Edmonton, AB, Canada
    J Neurochem 114:1405-13. 2010
    ..Increased formaldehyde (FA) and up-regulation of semicarbazide-sensitive amine oxidase, which forms FA from methylamine, have been ..
  56. pmc In organello formaldehyde crosslinking of proteins to mtDNA: identification of bifunctional proteins
    B A Kaufman
    Department of Molecular Biology, and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9148, USA
    Proc Natl Acad Sci U S A 97:7772-7. 2000 nucleoid proteins contribute to mtDNA organization and inheritance, we have developed an in organello formaldehyde crosslinking procedure to identify proteins associated with mtDNA...
  57. pmc Descending serotonergic facilitation of spinal ERK activation and pain behavior
    Camilla I Svensson
    Department of Anesthesiology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0818, USA
    FEBS Lett 580:6629-34. 2006
    ..These findings reveal that primary afferent-evoked activation of spinal ERK requires the input from an excitatory 5-HT descending pathway...
  58. pmc Extreme loss of immunoreactive p-Akt and p-Erk1/2 during routine fixation of primary breast cancer
    Isabel F Pinhel
    Department of Academic Biochemistry, Institute of Cancer Research, London, UK
    Breast Cancer Res 12:R76. 2010
    ..The aim of this study was to characterize the differences in immunoreactivity of common biomarkers that may occur (a) due to tissue handling at surgery and (b) between core-cuts and resected tumours...
  59. ncbi Influence of indoor factors in dwellings on the development of childhood asthma
    Joachim Heinrich
    Helmholtz Zentrum Munchen, National Research Center for Environmental Health, Institute of Epidemiology, Munich, Germany
    Int J Hyg Environ Health 214:1-25. 2011
    ..More research is also needed to clarify the potential risk for exposure to volatile and semi-volatile organic compounds due to renovation activities, phthalates and chlorine chemicals due to cleaning...
  60. ncbi Formaldehyde induces neurotoxicity to PC12 cells involving inhibition of paraoxonase-1 expression and activity
    Xiao Qing Tang
    Department of Physiology, Medical College, University of South China, Hengyang, Hunan, China
    Clin Exp Pharmacol Physiol 38:208-14. 2011
    1. Formaldehyde (FA) has been found to cause toxicity to neurons. However, its neurotoxic mechanisms have not yet been clarified...
  61. ncbi Role of altered rpoB alleles in Bacillus subtilis sporulation and spore resistance to heat, hydrogen peroxide, formaldehyde, and glutaraldehyde
    Ralf Moeller
    Institute of Aerospace Medicine, German Aerospace Center, Linder Hoehe, 51147 Cologne, Germany
    Arch Microbiol 194:759-67. 2012
  62. ncbi Biological role of formaldehyde, and cycles related to methylation, demethylation, and formaldehyde production
    Huba Kalasz
    Department of Pharmacology and Pharmacotherapy, Semmelweis University, H 1089 Budapest, Nagyvarad ter 4, Hungary
    Mini Rev Med Chem 3:175-92. 2003
    An overview is given on the analysis, formation, role and occurrence of formaldehyde in living organisms. Various methods have been used for the determination of formaldehyde in tissues and body fluids...
  63. ncbi Occupational exposures and cancer of the larynx-systematic review and meta-analysis
    Sophie Paget-Bailly
    INSERM, Center for Research in Epidemiology and Population Health, UMRS 1018, Occupational and Environmental Determinants of Health and Versailles Saint Quentin University, UMRS 1018, Villejuif, France
    J Occup Environ Med 54:71-84. 2012
    ..To review epidemiologic data on occupational exposures and laryngeal cancer...
  64. pmc Occupational exposure to formaldehyde and alterations in lymphocyte subsets
    H Dean Hosgood
    Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, Maryland 20892 7240, USA
    Am J Ind Med 56:252-7. 2013
    b>Formaldehyde is used in many occupational settings, most notably in manufacturing, health care, and embalming. Formaldehyde has been classified as a human carcinogen, but its mechanism of action remains uncertain.
  65. pmc Formaldehyde exposure and lower respiratory infections in infants: findings from the PARIS cohort study
    Célina Roda
    Universite Paris Descartes, Département Santé Publique et Biostatistiques EA 4064, Laboratoire Santé Publique et Environnement, Paris, France
    Environ Health Perspect 119:1653-8. 2011
    ..Although formaldehyde (FA) is one of the most common air pollutants found in indoor environments, its impact on infant health is ..
  66. ncbi Hydrogen sulfide prevents formaldehyde-induced neurotoxicity to PC12 cells by attenuation of mitochondrial dysfunction and pro-apoptotic potential
    Xiao Qing Tang
    Key Laboratory for Arteriosclerology of Hunan Province, University of South China, Hengyang 421001, Hunan, PR China
    Neurochem Int 61:16-24. 2012
    ..Numerous studies have demonstrated that exposure to formaldehyde (FA) causes neuronal damage and that oxidative stress is one of the most critical effects of FA exposure...
  67. pmc Identifying an indoor air exposure limit for formaldehyde considering both irritation and cancer hazards
    Robert Golden
    ToxLogic LLC, Potomac, Maryland 20854, USA
    Crit Rev Toxicol 41:672-721. 2011
    b>Formaldehyde is a well-studied chemical and effects from inhalation exposures have been extensively characterized in numerous controlled studies with human volunteers, including asthmatics and other sensitive individuals, which provide a ..
  68. ncbi Occupational exposure to formaldehyde: genotoxic risk evaluation by comet assay and micronucleus test using human peripheral lymphocytes
    Solange Costa
    National Institute of Health, Environmental Health Department, Porto, Portugal
    J Toxicol Environ Health A 74:1040-51. 2011
    b>Formaldehyde (FA) is a world high-production compound with numerous applications ranging from production of resins to medicines...
  69. ncbi Interaction between histamine and morphine at the level of the hippocampus in the formalin-induced orofacial pain in rats
    Esmaeal Tamaddonfard
    Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia 57153 1177, Iran
    Pharmacol Rep 63:423-32. 2011
    ..In addition, both histamine H(1) and H(2) receptors, as well as opioid receptors may be involved in the interaction between histamine and morphine in producing analgesia...
  70. pmc Primary central nervous system lymphomas: a validation study of array-based comparative genomic hybridization in formalin-fixed paraffin-embedded tumor specimens
    Esteban Braggio
    Department of Biochemistry, Mayo Clinic, Scottsdale, Arizona, USA
    Clin Cancer Res 17:4245-53. 2011
  71. ncbi Incomplete follow-up in the National Cancer Institute's formaldehyde worker study and the impact on subsequent reanalyses and causal evaluations
    Gary M Marsh
    Center for Occupational Biostatistics and Epidemiology and Department of Biostatistics, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Regul Toxicol Pharmacol 58:233-6. 2010
    ..performed reanalyses of the National Cancer Institute cohort study on nasopharyngeal cancer (NPC) risk among formaldehyde exposed workers (Hauptmann et al., 2004). Both reanalyses (Marsh and Youk, 2005; Marsh et al...
  72. pmc Use of LC-MS/MS and stable isotopes to differentiate hydroxymethyl and methyl DNA adducts from formaldehyde and nitrosodimethylamine
    Kun Lu
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Chem Res Toxicol 25:664-75. 2012
    b>Formaldehyde is a known human and animal carcinogen that forms DNA adducts, and causes mutations. While there is widespread exposure to formaldehyde in the environment, formaldehyde is also an essential biochemical in all living cells...
  73. pmc Random DNA fragmentation allows detection of single-copy, single-exon alterations of copy number by oligonucleotide array CGH in clinical FFPE samples
    Galen Hostetter
    Translational Genomics Research Institute, 445 N 5th Street, Phoenix, AZ 85004, USA
    Nucleic Acids Res 38:e9. 2010
    ..3. Finally we present data on derivative of log ratio, a particular sensitive detector of measurement variance, for 216 sequential hybridizations to assess protocol reliability over a wide range of FFPE samples...
  74. ncbi Formaldehyde and tobacco smoke as alkylating agents: the formation of N-methylenvaline in pathologists and in plastic laminate workers
    Roberto Bono
    Department of Public Health and Microbiology, University of Torino, Torino, Italy
    Sci Total Environ 414:701-7. 2012
    Aim of this study was to investigate the relationships between the concentration of formaldehyde in air and the alkylation of hemoglobin to form a terminal N-methylenvaline residue in three occupationally exposed groups: a) technicians of ..
  75. pmc Proteomic analysis of formalin-fixed paraffin-embedded tissue by MALDI imaging mass spectrometry
    Rita Casadonte
    Mass Spectrometry Research Center, Department of Biochemistry, Vanderbilt University, Nashville, Tennessee, USA
    Nat Protoc 6:1695-709. 2011
    ..For example, TMA analysis of 300 FFPE cores would typically require 6 h of total time through data acquisition, not including data analysis...
  76. pmc Mortality from lymphohematopoietic malignancies among workers in formaldehyde industries: the National Cancer Institute Cohort
    Laura E Beane Freeman
    Occupational and Environmental Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    J Natl Cancer Inst 101:751-61. 2009
    b>Formaldehyde exposure is associated with leukemia in some epidemiological studies...
  77. pmc Multicentre validation study of nucleic acids extraction from FFPE tissues
    Serena Bonin
    ACADEM Department University of Trieste and ICGEB, University of Trieste, Cattinara Hospital, 34149 Trieste, Italy
    Virchows Arch 457:309-17. 2010
    ..The results of the study emphasize the need for quality control of the nucleic acid extracts with standardised methods to prevent false negative results and to allow data comparison among different diagnostic laboratories...
  78. ncbi Producing reverse phase protein microarrays from formalin-fixed tissues
    Claudia Wolff
    Institut fur Pathologie, Technische Universitat Munchen, Munich, Germany
    Methods Mol Biol 785:123-40. 2011
    ..In this chapter, we describe a protein extraction protocol established in our laboratory combined with the use of reverse phase protein microarray...
  79. ncbi Proteome, phosphoproteome, and N-glycoproteome are quantitatively preserved in formalin-fixed paraffin-embedded tissue and analyzable by high-resolution mass spectrometry
    Paweł Ostasiewicz
    Department for Proteomics and Signal Transduction at Max Planck Institute for Biochemistry, 82152 Martinsried, Germany
    J Proteome Res 9:3688-700. 2010
    ..Thus, FFPE biobank material can be analyzed by quantitative proteomics at the level of proteins and post-translational modifications...
  80. ncbi Combined analysis of chromosomal aberrations and glutathione S-transferase M1 and T1 polymorphisms in pathologists occupationally exposed to formaldehyde
    Alfredo Santovito
    Department of Animal and Human Biology, University of Torino, Via Accademia Albertina n 13, 10123, Turin, Italy
    Arch Toxicol 85:1295-302. 2011
    The formaldehyde (FA) genotoxic potential in occupationally exposed individuals is conflicting...
  81. ncbi Genotoxicity biomarkers in occupational exposure to formaldehyde--the case of histopathology laboratories
    Carina Ladeira
    Escola Superior de Tecnologia da Saúde de Lisboa, Instituto Politécnico de Lisboa, Portugal
    Mutat Res 721:15-20. 2011
    b>Formaldehyde, classified by the IARC as carcinogenic in humans and experimental animals, is a chemical agent that is widely used in histopathology laboratories...
  82. ncbi Histology without formalin?
    René J Buesa
    Ann Diagn Pathol 12:387-96. 2008
    ..With a fast penetration, formaldehyde is a slow and reversible fixative that requires 24 to 48 hours to completely bind to tissue; thus, any surgical ..
  83. pmc Evaluation and validation of total RNA extraction methods for microRNA expression analyses in formalin-fixed, paraffin-embedded tissues
    Martina Doleshal
    Asuragen, Inc, Austin, TX 78744, USA
    J Mol Diagn 10:203-11. 2008
  84. pmc Genome-wide comparison of paired fresh frozen and formalin-fixed paraffin-embedded gliomas by custom BAC and oligonucleotide array comparative genomic hybridization: facilitating analysis of archival gliomas
    Gayatry Mohapatra
    Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
    Acta Neuropathol 121:529-43. 2011
    ..In combination, these advances should facilitate genome-wide analysis of rare, small and/or histologically heterogeneous gliomas from FFPE tissues...
  85. ncbi Treatment of maternal blood samples with formaldehyde does not alter the proportion of circulatory fetal nucleic acids (DNA and mRNA) in maternal plasma
    Satheesh Kumar Reddy Chinnapapagari
    Department of Research, University Women s Hospital, Basel, Switzerland
    Clin Chem 51:652-5. 2005
  86. ncbi Lack of dramatic enrichment of fetal DNA in maternal plasma by formaldehyde treatment
    Grace T Y Chung
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong, China
    Clin Chem 51:655-8. 2005
  87. pmc Enhanced formaldehyde detoxification by overexpression of glutathione-dependent formaldehyde dehydrogenase from Arabidopsis
    Hakima Achkor
    Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias, Universidad Autonoma de Barcelona, 08193 Bellaterra, Barcelona, Spain
    Plant Physiol 132:2248-55. 2003
    The ADH2 gene codes for the Arabidopsis glutathione-dependent formaldehyde dehydrogenase (FALDH), an enzyme involved in formaldehyde metabolism in eukaryotes...
  88. ncbi The generation of formaldehyde in cigarettes--Overview and recent experiments
    Richard R Baker
    Group Research and Development, British American Tobacco, Regent s Park Road, Southampton SO15 8TL, UK
    Food Chem Toxicol 44:1799-822. 2006
    ..One exception to this generalisation is formaldehyde, which is generated from saccharides used as tobacco ingredients...
  89. ncbi Clinical proteomic applications of formalin-fixed paraffin-embedded tissues
    Josip Blonder
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, National Cancer Institute at Frederick, 1050 Boyles Street, Frederick, MD 21702, USA
    Clin Lab Med 29:101-13. 2009
    ..The almost inexhaustible supply of these tissues will enable proteomic laboratories access to clinically important specimens that will undoubtedly lead to a number of important discoveries in the near future...
  90. ncbi Whole genome SNP arrays using DNA derived from formalin-fixed, paraffin-embedded ovarian tumor tissue
    Ella R Thompson
    VBCRC Cancer Genetics Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia
    Hum Mutat 26:384-9. 2005
    ..This advance will open up vast archival tissue resources to high-resolution genetic analysis and unlock a wealth of biological information...
  91. pmc Quality assessment of DNA derived from up to 30 years old formalin fixed paraffin embedded (FFPE) tissue for PCR-based methylation analysis using SMART-MSP and MS-HRM
    Lasse S Kristensen
    Institute of Human Genetics, University of Aarhus, The Bartholin Building, Aarhus C, Denmark
    BMC Cancer 9:453. 2009
    ..Sensitive Melting Analysis after Real Time - Methylation Specific PCR (SMART-MSP) and Methylation Sensitive - High Resolution Melting (MS-HRM) are two methods for single locus DNA methylation detection based on HRM...
  92. ncbi Formaldehyde: integrating dosimetry, cytotoxicity, and genomics to understand dose-dependent transitions for an endogenous compound
    Melvin E Andersen
    Program in Chemical Safety Sciences, The Hamner Institutes for Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Toxicol Sci 118:716-31. 2010
    b>Formaldehyde (FA), an endogenous cellular aldehyde, is a rat nasal carcinogen...
  93. ncbi Quantitation of p95HER2 in paraffin sections by using a p95-specific antibody and correlation with outcome in a cohort of trastuzumab-treated breast cancer patients
    Jeff Sperinde
    diaDexus, South San Francisco, CA, USA
    Clin Cancer Res 16:4226-35. 2010
    ..We sought to show that quantitative p95HER2 levels would correlate with outcome in trastuzumab-treated HER2-positive metastatic breast cancer...
  94. pmc The function neutralizing anti-TrkA antibody MNAC13 reduces inflammatory and neuropathic pain
    Gabriele Ugolini
    Lay Line Genomics, Via di Castel Romano 100, 00128 Rome, Italy
    Proc Natl Acad Sci U S A 104:2985-90. 2007
    ..This study represents a direct demonstration that neutralizing antibodies directed against the TrkA receptor may display potent analgesic effects in inflammatory and chronic pain...
  95. ncbi Epidemiological studies of formaldehyde exposure and risk of leukemia and nasopharyngeal cancer: a meta-analysis
    Annette M Bachand
    Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, Colorado, USA
    Crit Rev Toxicol 40:85-100. 2010
    The authors conducted meta-analyses of the epidemiological literature on formaldehyde exposure and risk of leukemia and risk of nasopharyngeal cancer...
  96. ncbi Differential contributions of the basolateral and central nuclei of the amygdala in the negative affective component of chemical somatic and visceral pains in rats
    Sachi Tanimoto
    Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606 8501, Japan
    Eur J Neurosci 18:2343-50. 2003
    ..Taken together, these results suggest that activation of distinct amygdaloid nuclei could differentially contribute to chemical somatic and visceral noxious stimuli-induced negative affective, but not sensory components of pains...
  97. pmc Molecular dosimetry of N2-hydroxymethyl-dG DNA adducts in rats exposed to formaldehyde
    Kun Lu
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, North Carolina 27599, United States
    Chem Res Toxicol 24:159-61. 2011
    ..7, 2, 5.8, 9.1, or 15.2 ppm [(13)CD(2)] formaldehyde for 6 h were quantified by a highly sensitive nano-UPLC-MS/MS method...
  98. ncbi An ion channel essential for sensing chemical damage
    Lindsey J Macpherson
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Neurosci 27:11412-5. 2007
    Tissue damage and its downstream consequences are experimentally assayed by formaldehyde application, which indiscriminately modifies proteins and is presumed to cause pain through broadly acting mechanisms...
  99. ncbi DNA extraction from archival formalin-fixed, paraffin-embedded tissues: heat-induced retrieval in alkaline solution
    Shan Rong Shi
    Department of Pathology, University of Southern California Keck School of Medicine HMR 204, 2011 Zonal Avenue, Los Angeles 90033, USA
    Histochem Cell Biol 122:211-8. 2004
    ..1 M alkaline solution is a simpler and more effective heat-induced retrieval protocol for DNA extraction. Combination with some chemicals (detergents) may further significantly improve efficiency of the heat-induced retrieval technique...
  100. pmc Comparison of miRNA expression patterns using total RNA extracted from matched samples of formalin-fixed paraffin-embedded (FFPE) cells and snap frozen cells
    Jinghuan Li
    Department of Histopathology, University of Dublin, Trinity College, Dublin, Ireland
    BMC Biotechnol 7:36. 2007
    ..This study analyzed 160 miRNAs in paired snap frozen and FFPE cells to investigate if miRNAs may be successfully detected in archival specimens...
  101. pmc An array-based analysis of microRNA expression comparing matched frozen and formalin-fixed paraffin-embedded human tissue samples
    Xiao Zhang
    Department of Pathology and Molecular Medicine, Queen s Laboratory for Molecular Pathology, Kingston, ON, Canada
    J Mol Diagn 10:513-9. 2008
    ..669 to 0.815; Spearman rank correlation coefficient, 0.847 to 0.948), supporting the use of FFPE-derived miRNAs for array-based, gene expression profiling...

Research Grants78

  1. Aberrant DNA Methylation of MicroRNA Genes in Hepatocellular Carcinoma (HCC)
    Jing Shen; Fiscal Year: 2012
    ..PHS 398/2590 (Rev. 06/09) Page Continuation Format Page ..
  2. microRNAs as novel biomarkers for management of breast cancer
    LORENZO SEMPERE; Fiscal Year: 2012
    ..Our results should provide a solid foundation to develop miRNA-based clinical assays to assist physicians in making crucial decisions with regards to treatment of patients. ..
  3. Sleep in Rats: From Conceptualization to Measurement, Analysis and Interpretation
    Gayle Giboney Page; Fiscal Year: 2010
    ..The relevance of this proposed award to human health is to discern the contributions and consequences of disrupted sleep as it relates to the development of persistent pain. ..
  4. Validation and Quantification of FFPE Antigen Retrieval by Proteome Analysis
    XUPEING FANG; Fiscal Year: 2010
  5. Role of TRP Channels in Environmental Irritant-Induced Headache
    Gerry S Oxford; Fiscal Year: 2013
    ..most common symptom associated with indoor and outdoor air pollution but how environmental irritants, such as formaldehyde and acrolein, cause headache is not known...
  6. Carboxylesterase-activated Doxazolidine-prodrug for Hepatocellular Carcinoma
    Tad H Koch; Fiscal Year: 2012
    ..The therapy is based upon doxazolidine, a formaldehyde conjugate of doxorubicin that is more than an order of magnitude more active against a variety of both ..
  7. Biological &Environmental Mediators of Vitamin D and Aggressive Prostate Cancer
    ADAM BRYANT MURPHY; Fiscal Year: 2013
  8. Scleroderma Subtyping from Fresh and Archived Biopsies using NextGen Sequencing
    Michael W Fanger; Fiscal Year: 2011
    ..The goal of this project is to simplify the currently complex sample preparation protocol while improving the robustness and decreasing the cost of the genetic analyses. ..
  9. Placental Treg Activation and Pregnancy Outcome in the FIV-infected Cat Model
    GREGORY TODD PHARR; Fiscal Year: 2010
    ..The purpose of this project is to evaluate the effect of HIV infection on the function of regulatory T cells, using the feline immunodeficiency virus (FIV)-infected cat to model maternal-fetal infection and compromised pregnancy. ..
  10. A Mucosal RSV Vaccine
    Kejian Yang; Fiscal Year: 2013
  11. Environmental and genetic regulation of copy number variation (CNV)
    THOMAS PETES; Fiscal Year: 2010
    ..will use a cassette that contains two yeast genes SFA1 and CUP1 that confer gene dosage-dependent tolerance to formaldehyde and copper, respectively...
  12. Chromatin Immunoprecipitation (ChIP) Assay Development for FFPE human brain secti
    Mary Anne Jelinek; Fiscal Year: 2011
    ..Phase I proposal , we intend to develop a Clinical Chromatin ImmunoPrecipitation assay (C- ChIP), for use with formaldehyde-fixed paraffin embedded (FFPE) human brain specimens, which will enable analysis of changes in histone post-..
  13. Gene Expression Patterns in Human Tumors Identified Using Transcript Sequencing
    Charles M Perou; Fiscal Year: 2013
    ..We will use FAIRE (Formaldehyde-Assisted isolation of Regulatory Elements), a simple, low-cost method amenable to use on small quantities of ..
  14. Investigating the role of Bacillithiol in metal ion homeostasis and the reactive
    Pete Chandrangsu; Fiscal Year: 2013
    ..In Aim 1, the role of BSH in the response to the reactive electrophiles, methylglyoxal (MG) and formaldehyde (FA) will be examined. MG and FA are endogenously produced products of metabolism...
  15. Implementation of phosphoprotein preservation technology for cancer biospecimens
    LANCE ALLEN LIOTTA; Fiscal Year: 2013
    ..This would increase diagnostic accuracy while substantially reducing costs. ..
  16. miRNA HD Array Platform
    Bruce E Seligmann; Fiscal Year: 2010
    ..In Phase II, this platform will be applied to identify host cell miRNA signatures useful for screening for infection by Category A, B and C pathogens, and useful as targets for drug discovery. ..
  17. Nanopore Biosensor for Kinetics of Reparative Antibodies on Plasma Membranes
    Sang Hyun Oh; Fiscal Year: 2013
    ..Fixation of any kind (methanol, formaldehyde, freezing) destroys the complex membrane antigen...
  18. Acquisition of laser capture microdissection microscope
    Marc H Symons; Fiscal Year: 2010
    ..A financial plan is in place to recover maintenance costs. Utilization of the LCM system by NIH-funded investigators will be prioritized and efforts will be made to introduce LCM technology and its application to new investigators. ..
  19. Identifying disease-related functional regulatory variants in open chromatin
    Karen L Mohlke; Fiscal Year: 2010
    ..Successful genome-wide experimental approaches to identify open chromatin include DNase hypersensitivity (DHS), formaldehyde-assisted identification of regulatory elements (FAIRE), markers of histone modification, and chromatin ..
  20. Improving Antibodies to Virion Based HIV Vaccines
    Kathie Grovit-Ferbas; Fiscal Year: 2013
    ..To this end, we have used Env modified formaldehyde and heat treated virions and pseudovirions as tools to study correlates of Ab mediated protection...
  21. Accelerating Cancer Research with Single Cell Arrays
    Heinz Ulrich Weier; Fiscal Year: 2009
    ..This will open new avenues for the analysis of small samples like those obtained by fine needle biopsies as well as the analysis of circulating or exfoliated tumor cells. ..
  22. Epigenetic regulation of vascular smooth muscle cell phenotype
    Christopher P Mack; Fiscal Year: 2013
    ..with Jason Lieb and Terry Furrey to "map" open chromatin regions in SMC using DNase hypersensitivity and Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE)...
  23. Adenovirus-vectored RSV vaccine not inhibited by maternal immunity
    JASON GRAHAM GALL; Fiscal Year: 2013
    ..In phase I we will test the hypothesis that RSV-specific immune responses induced by a novel replication-deficient adenovirus vector derived from non-human adenovirus will not be inhibited by human viral immunity. ..
  24. A high-resolution in situ proteomics atlas of salivary gland development
    Melinda Larsen; Fiscal Year: 2010
    ..These studies will provide insight into the fundamental signaling events occurring during the process of salivary gland development and differentiation. ..
  25. Automated Macrodissection Device
    Nils B Adey; Fiscal Year: 2012
    ..The new device will promote productivity and quality of results for clinicians working in cancer diagnostics, as well as diverse areas of cancer and non-cancer research requiring high throughput tissue dissection from slides. ..
  26. Synthetic nanoparticle vaccines for RSV
    THOMAS J POWELL; Fiscal Year: 2012
    ..The application of this innovative approach to RSV vaccine development will also impact vaccine development for other infectious diseases. ..
  27. miRNA Biomarkers from FFPE Tissue for Early Breast Cancer Detection
    Sidney W Fu; Fiscal Year: 2013
    ..The success of this project would have a broad impact in research of other diseases, health disparities and policy making due to the world-wide availability of FFPE samples with rich clinical data. ..
  28. Adenosine Receptors and Ethanol Withdrawal
    TRACY RENEE BUTLER; Fiscal Year: 2010
  29. New biomonitoring methodologies to measure DNA adducts in human tissues
    Robert J Turesky; Fiscal Year: 2013
    ..These novel screening techniques will be employed to measure AA-DNA adducts in human populations exposed to AA and diagnosed with upper urothelial cancer. ..
    SATHYANARAYANA N MURTHY; Fiscal Year: 2013
    ..abstract_text> ..
  31. DNA-Methylation Profiling from Fixed Melanocytic Tissues
    NANCY E contact THOMAS; Fiscal Year: 2010
    ..This study will be a first step toward the development of diagnostic methylation assays that could be used to standardize melanoma diagnosis, thereby decreasing under- and over-treatment of melanocytic lesions. ..
  32. Estrogen regulation of inflammatory mediator signaling
    William P Clarke; Fiscal Year: 2011
    ..This work will provide a basis for understanding the enhanced sensitivity of women to pain and provide new targets for improved pain pharmacotherapy. ..
  33. The Role of TLR4 and RSV F Protein in Immunity to RSV
    JORGE C BLANCO; Fiscal Year: 2013
    ..It is expected that at the completion of this grant, we will have identified strategies that may lead to development of a RSV vaccine and new therapeutics for mitigating the pathologic host response to RSV. ..
  34. Determinants of Efficacy to the Akt Inhibitor Perifosine in Colorectal Cancer
    Cathy Eng; Fiscal Year: 2012
    ..Endpoint: Pharma- codynamic biomarker changes will correlate with efficacy of perifosine therapy. ..
  35. CaMKII as a Mechanism &Intervention Target for Sickle Cell Pain
    Zaijie Jim Wang; Fiscal Year: 2013
    ..The significance of this proposal is that it may ultimately lead to pharmacological interventions that target the CaMKII-pathway. (End of Abstract) ..
  36. High-Throughput DNA-Methylation Profiling from Fixed Melanocytic Tissues
    Nancy E Thomas; Fiscal Year: 2013
  37. Biomarkers of response to Hsp90 inhibitors in triple-negative breast cancer
    Gabriela Chiosis; Fiscal Year: 2013
  38. Molecular signatures of melanoma histology and progression: A Population Based A
    Abrar A Qureshi; Fiscal Year: 2013
    ..Evaluate genome-wide (6,000 genes) association with BRAF/NRAS/MC1R status and melanoma recurrence and mortality due to melanoma ..
  39. Novel Biomarkers for Aromatase Inhibitor Therapy
    Matthew J Ellis; Fiscal Year: 2013
  40. Development of a CES 2-Activated Doxazolidine Prodrug for Pancreatic Cancer
    Tad H Koch; Fiscal Year: 2010
    ..The therapy is based upon doxazolidine, a formaldehyde conjugate of doxorubicin that is more than an order of magnitude more active against a variety of both ..
  41. Experimental evolution recapitulating adaptation after horizontal gene transfer
    JOSHUA KIERAN MICHENER; Fiscal Year: 2013
    ..DCM will suggest possible phenotypical determinants of DCM growth, such as tolerance to by-products including formaldehyde and hydrochloric acid...
  42. Quantitative molecular profiling of tumor biomarkers with multi-color monovalent
    Hongyan Liu; Fiscal Year: 2012
    ..Our research will be relevant to the mission of the NIH and be broadly interesting to researchers studying the pathology of variety diseases. ..
  43. Molecular profiling of HIV-associated lymphoma in the US and Malawi
    Satish Gopal; Fiscal Year: 2013
    ..The molecular insights gained will substantially illuminate lymphoma biology in the context of HIV, and result in improved treatments for this challenging and vulnerable population in both resource-rich and resource-limited settings. ..
  44. Comprehensive Molecular Diagnosis for Food and Waterborne Enteropathogens
    Eric R Houpt; Fiscal Year: 2011
  45. Methods and Instrumentation for Hydrostatic Pressure-Enhanced Tissue Fixation
    Alexander V Lazarev; Fiscal Year: 2011
    ..High pressure has been shown to enhance rapid and uniform formaldehyde penetration into tissue blocks...
  46. Detection of Light Chain Restriction in non-Hodgkin Lymphoma by a Novel RNA In Si
    Xiao Jun Ma; Fiscal Year: 2012
    ..Detection of restricted immunoglobulin light chain expression is a molecular ancillary tool to resolve ambiguous lymphoproliferative disorders. The proposed product based on RNAscope will provide superior sensitivity to current methods. ..
  47. Prognostic Models in Diffuse Large B-Cell Lymphoma
    Izidore S Lossos; Fiscal Year: 2010
    ..Further, establishment of a paraffin-based RNA prognostic model using the new methodology of RNA extraction could serve as a paradigm for other lymphomas and tumors. ..
  48. Single-molecule nanomagnetic assays for ultrasmall sample clinical diagnostics
    Dmitri Litvinov; Fiscal Year: 2010
    ..This research is expected to enable a new generation of highly reliable molecular diagnostic instrumentation with significantly enhanced sensitivity and high specificity. ..
  49. Neonatal Pain, Depression and Pain Susceptibility at Maturity in Rats
    Gayle Giboney Page; Fiscal Year: 2013
  50. Mechanisms of stromal fibroblast activation in esophageal tumors
    TODD JOSEPH WALDRON; Fiscal Year: 2012
    ..In aggregate, fulfillment of the aims described above will provide insight into mechanisms of activation of CAFs by MDSCs, and determine whether MDSCs are required for intiation and progression of esophageal cancer. ..
  51. Rift Valley Fever Vaccine Development of Animal Models and Optimize Production
    Clarence J Peters; Fiscal Year: 2010
    ..No clinical trials are in this proposal. ..
  52. Low-Cost Catalytic Biomass Cookstove for Improved Indoor Air Quality
    PAUL YELVINGTON; Fiscal Year: 2013
    ..Emissions of CO, unburned hydrocarbons (including air toxics like formaldehyde) and particulate matter (PM) are particularly problematic...
  53. True Direct Single Molecule RNA Sequencing
    PATRICE MARIE MILOS; Fiscal Year: 2012
    ..We will improve tDRS by: 1) screening and synthesis of modified nucleotides for superior performance, and 2) screening and mutagenesis of polymerases for optimal behavior. ..
  54. Formaldehyde and Other Pollutants from Ozone Reactions on Ventilation Systems
    Hugo Destaillats; Fiscal Year: 2010
    ..purpose of this project is to improve understanding of the formation of harmful gas- phase pollutants, such as formaldehyde, and aerosol particles in the indoor work environment...
  55. A Highly Multiplexed PCR Platform for Gene Expression Profiling from FFPE Tissue
    JORK N NOLLING; Fiscal Year: 2012
    John Taylor; Fiscal Year: 2009
    ..between HDV RNAs and proteins with host components: Cells undergoing HDV replication will be fixed with formaldehyde as an approach to detect interactions of delta protein with HDV and host RNAs...
  57. Therapeutic Opportunities for Pediatric Astrocytoma
    Rosalind A Segal; Fiscal Year: 2013
    ..with BRAF? An innovative feature of this project is recently developed methods for genetic profiling of formaldehyde-fixed, paraffin-embedded samples...
  58. Epigenetic Actions of Growth Hormone
    Dennis J Chia; Fiscal Year: 2012
    ..Standard molecular biology techniques, including chromatin immunoprecipitation (ChIP) assay and the derivative formaldehyde-assisted isolation of regulatory elements (FAIRE), will be employed on samples prepared from the livers of mice ..
  59. Molecular Diagnosis and Prognosis in Aggressive Lymphoma
    Lisa Rimsza; Fiscal Year: 2013
  60. Translation of Predictive Cancer Biomarkers into Clinical Practice
    TIMOTHY J contact TRICHE; Fiscal Year: 2010
  61. Efficacy of BCG Therapy is a Function of Bladder Tumor Immune Microenvironment
    JAMES JOSEPH LEE; Fiscal Year: 2013
    ..Our expectation is that the definition of Th2 immune responses in bladder tumors may also lead to previously untested/novel therapeutic modalities. ..
  62. CYP2D6 gene variants and effectiveness of adjuvant tamoxifen in breast cancer
    Sheila A Weinmann; Fiscal Year: 2013
    ..Our ultimate goal is to learn more about the genetic factors underlying breast cancer treatment response in order to make breast cancer treatment more targeted and more effective. ..
    GHULAM ANSARI; Fiscal Year: 1991
    ..Industrial chemicals containing functional groups such as aldehyde (acrolein, formaldehyde, and glutaraldehyde), epoxide (ethylene oxide, propylene oxide, and styrene oxide), and hydrocarbon (benzene, ..
  64. Innate immune evasion and/or antagonism by eastern equine encephalitis virus
    KATHERINE RYMAN; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
    Lora Fleming; Fiscal Year: 2001
    ..routinely exposed to a variety of carcinogenic substances, such as benzene, polycyclic aromatic hydrocarbons, formaldehyde, and asbestos...
  66. Developing an inactivated whole cell H. pylori vaccine
    GARY NABORS; Fiscal Year: 2006
    ..This trial will position the vaccine for more highly powered Phase 1 or early Phase 2 trials, and thus will move H. pylori vaccination one step closer to being a reality. ..
  67. Immunoprotective monoclonals to B. anthracis spores
    ALISON O BRIEN; Fiscal Year: 2003
    ..actually bind to the surface of spores and decrease the level of spore germination, taken with the fact that formaldehyde-inactivated spores can serve as a protective vaccine against anthrax in guinea pigs, led us to the following ..
    Marc Coltrera; Fiscal Year: 1993
    ..As none of the currently available proliferation-specific monoclonal antibodies can be used in formaldehyde-fixed, paraffin-embedded tissues, new proliferation-specific monoclonal antibodies capable of reacting with S-..
  69. Early Diagnosis of Oral Carcinoma Using Mutant PP32R1
    GERALD KOCHEVAR; Fiscal Year: 2006
    ..Identification of the intracellular protein ligands will provide a specific functional context for mutant PP32R1S. ..
  70. RSV Upper Airway Infection and Otitis Media
    JOAN E contact DURBIN; Fiscal Year: 2010
    ..Our group has found a new vaccine against this virus, and want to test whether immunity to RSV can protect against the process that causes bacterial ear infections. ..
    Bradley Taylor; Fiscal Year: 2002
    ..The results of these studies will facilitate our understanding of the mechanisms that contribute to chronic pain in the hypertensive patient. ..
    Lynn Dressler; Fiscal Year: 2005
    ..Two subcontracts are included in this proposal, one to support the CALGB Pathology Coordinating Office functions at OSU relevant to this study and the other in support of the molecular studies being performed by USC. ..
    VERONICA BIERBAUM; Fiscal Year: 2000
    ..Preliminary studies indicate that formaldehyde plays a critical role in this mechanism, and that newly synthesized anthracycline-formaldehyde conjugates ..
    Mary Lidstrom; Fiscal Year: 2007
    ..A distinguishing feature of methylotrophic metabolism is the generation and consumption of formaldehyde as a central intermediate, the starting point for all of metabolism...
  75. Develop & Validate An Ultrasound Tissue Preservation De*
    Zhenqing Zhu; Fiscal Year: 2006
    ..Long-term stability of tissue morphology and mRNA integrity in USFFPE tissues is slightly better than that in conventional FFPE tissues. [unreadable] [unreadable]..
    SHALABY SHALABY; Fiscal Year: 2000
    ..of a novel radiochemical sterilization (RC-S) process comprising a combination of a controllably released formaldehyde, and low-dose (equal to or less than 7...
  77. In vivo RNAi nanoparticle cancer gene function reagent
    Martin Woodle; Fiscal Year: 2009
    ..If successful, the resulting reagent is expect to enable rapid advances in understanding this life threatening disease that produce better targeted therapeutic treatment options for patients. ..
    R Armstrong; Fiscal Year: 1992
    ..The specific aims are to: 1) test the hypothesis that occupational exposure to specific dusts, smokes, formaldehyde, and aromatic hydrocarbons in Selangor Malaysia are risk factors for NPC; 2) to estimate risk of NPC ..