myosin heavy chains

Summary

Summary: The larger subunits of MYOSINS. The heavy chains have a molecular weight of about 230 kDa and each heavy chain is usually associated with a dissimilar pair of MYOSIN LIGHT CHAINS.

Top Publications

  1. ncbi Spatiotemporal gene expression targeting with the TARGET and gene-switch systems in Drosophila
    Sean E McGuire
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Sci STKE 2004:pl6. 2004
  2. ncbi Plasma membrane compartmentalization in yeast by messenger RNA transport and a septin diffusion barrier
    P A Takizawa
    Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, CA 94143, USA
    Science 290:341-4. 2000
  3. ncbi Ca2+ transients activate calcineurin/NFATc1 and initiate fast-to-slow transformation in a primary skeletal muscle culture
    Hans Peter Kubis
    Zentrum Physiologie, Medizinische Hochschule Hannover, D 30625 Hannover, Germany
    Am J Physiol Cell Physiol 285:C56-63. 2003
  4. ncbi Alpha-myosin heavy chain: a sarcomeric gene associated with dilated and hypertrophic phenotypes of cardiomyopathy
    Elisa Carniel
    Familial Cardiomyopathy Registry Research Group, USA
    Circulation 112:54-9. 2005
  5. pmc A family of microRNAs encoded by myosin genes governs myosin expression and muscle performance
    Eva van Rooij
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9148, USA
    Dev Cell 17:662-73. 2009
  6. pmc Association of trypanolytic ApoL1 variants with kidney disease in African Americans
    Giulio Genovese
    Division of Nephrology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA
    Science 329:841-5. 2010
  7. ncbi Localization of ASH1 mRNA particles in living yeast
    E Bertrand
    Department of Anatomy and Structural Biology and Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Mol Cell 2:437-45. 1998
  8. ncbi Control of stress-dependent cardiac growth and gene expression by a microRNA
    Eva van Rooij
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9148, USA
    Science 316:575-9. 2007
  9. ncbi Mutations in myosin heavy chain 11 cause a syndrome associating thoracic aortic aneurysm/aortic dissection and patent ductus arteriosus
    Limin Zhu
    Assistance Publique Hopitaux de Paris, Hopital Europeen Georges Pompidou, Département de génétique, 75015 Paris, France
    Nat Genet 38:343-9. 2006
  10. pmc MicroRNA let-7f inhibits tumor invasion and metastasis by targeting MYH9 in human gastric cancer
    Shuli Liang
    State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital of Digestive Diseases, Xi an, China
    PLoS ONE 6:e18409. 2011

Detail Information

Publications415 found, 100 shown here

  1. ncbi Spatiotemporal gene expression targeting with the TARGET and gene-switch systems in Drosophila
    Sean E McGuire
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Sci STKE 2004:pl6. 2004
    ..In this protocol, we provide guidelines for the use of these two novel systems, which should have general utility in studying Drosophila biology and in using the fly as a model for human disease...
  2. ncbi Plasma membrane compartmentalization in yeast by messenger RNA transport and a septin diffusion barrier
    P A Takizawa
    Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, CA 94143, USA
    Science 290:341-4. 2000
    ..These results indicate that yeast creates distinct plasma membrane compartments, as has been described in neurons and epithelial cells...
  3. ncbi Ca2+ transients activate calcineurin/NFATc1 and initiate fast-to-slow transformation in a primary skeletal muscle culture
    Hans Peter Kubis
    Zentrum Physiologie, Medizinische Hochschule Hannover, D 30625 Hannover, Germany
    Am J Physiol Cell Physiol 285:C56-63. 2003
    ....
  4. ncbi Alpha-myosin heavy chain: a sarcomeric gene associated with dilated and hypertrophic phenotypes of cardiomyopathy
    Elisa Carniel
    Familial Cardiomyopathy Registry Research Group, USA
    Circulation 112:54-9. 2005
    ..In failing human hearts, downregulation of alphaMyHC mRNA or protein has been correlated with systolic dysfunction. We hypothesized that mutations in alphaMyHC could also lead to pleiotropic cardiac phenotypes, including HCM and DCM...
  5. pmc A family of microRNAs encoded by myosin genes governs myosin expression and muscle performance
    Eva van Rooij
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9148, USA
    Dev Cell 17:662-73. 2009
    ....
  6. pmc Association of trypanolytic ApoL1 variants with kidney disease in African Americans
    Giulio Genovese
    Division of Nephrology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA
    Science 329:841-5. 2010
    ..We speculate that evolution of a critical survival factor in Africa may have contributed to the high rates of renal disease in African Americans...
  7. ncbi Localization of ASH1 mRNA particles in living yeast
    E Bertrand
    Department of Anatomy and Structural Biology and Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Mol Cell 2:437-45. 1998
    ..Video microscopy demonstrated that She1p/Myo4p moved particles to the bud tip at 200-440 nm/sec. Therefore, the ASH1 3'UTR-dependent particle serves as a marker for RNA transport and localization...
  8. ncbi Control of stress-dependent cardiac growth and gene expression by a microRNA
    Eva van Rooij
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9148, USA
    Science 316:575-9. 2007
    ..Thus, the alphaMHC gene, in addition to encoding a major cardiac contractile protein, regulates cardiac growth and gene expression in response to stress and hormonal signaling through miR-208...
  9. ncbi Mutations in myosin heavy chain 11 cause a syndrome associating thoracic aortic aneurysm/aortic dissection and patent ductus arteriosus
    Limin Zhu
    Assistance Publique Hopitaux de Paris, Hopital Europeen Georges Pompidou, Département de génétique, 75015 Paris, France
    Nat Genet 38:343-9. 2006
    ..Human MYH11 gene mutations provide the first example of a direct change in a specific SMC protein leading to an inherited arterial disease...
  10. pmc MicroRNA let-7f inhibits tumor invasion and metastasis by targeting MYH9 in human gastric cancer
    Shuli Liang
    State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital of Digestive Diseases, Xi an, China
    PLoS ONE 6:e18409. 2011
    ..In this study, we investigate whether let-7f acts as a tumor suppressor to inhibit invasion and metastasis in gastric cancers...
  11. pmc Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast
    Jian Qiu Wu
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    J Cell Biol 174:391-402. 2006
    ..Their absence delays late steps in cytokinesis, including septum formation and cell separation...
  12. ncbi Muscle fibre type adaptation in the elderly human muscle
    Jesper L Andersen
    Copenhagen Muscle Research Center, Department of Molecular Muscle Biology, Rigshospitalet, Copenhagen, Denmark
    Scand J Med Sci Sports 13:40-7. 2003
    ....
  13. pmc Involvement of an actomyosin contractile ring in Saccharomyces cerevisiae cytokinesis
    E Bi
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    J Cell Biol 142:1301-12. 1998
    ..cerevisiae. In its absence, cytokinesis can still be completed by a process (possibly localized cell-wall synthesis leading to septum formation) that appears to require septin function and to be facilitated by F-actin...
  14. ncbi Identification of separate slow and fast muscle precursor cells in vivo, prior to somite formation
    S H Devoto
    Institute of Neuroscience, University of Oregon, Eugene 97403, USA
    Development 122:3371-80. 1996
    ..Thus, slow and fast muscle fiber types in zebrafish axial muscle arise from distinct populations of cells in the segmental plate that develop in different cellular environments and display distinct behaviors...
  15. ncbi Targeted localization of Inn1, Cyk3 and Chs2 by the mitotic-exit network regulates cytokinesis in budding yeast
    Franz Meitinger
    German Cancer Research Centre, DKFZ ZMBH Alliance, Molecular Biology of Centrosomes and Cilia Unit, Im Neuenheimer Feld 581, 69120 Heidelberg, Germany
    J Cell Sci 123:1851-61. 2010
    ..This defect was partially suppressed by increased levels of Cyk3. We therefore propose that the MEN directly controls cytokinesis via targeting of Inn1, Cyk3 and Chs2 to the bud neck...
  16. pmc Missense mutations in the APOL1 gene are highly associated with end stage kidney disease risk previously attributed to the MYH9 gene
    Shay Tzur
    Ruth and Bruce Rappaport Faculty of Medicine and Research Institute, Technion Israel Institute of Technology, Haifa, Israel
    Hum Genet 128:345-50. 2010
    ....
  17. pmc Polymorphisms in MYH9 are associated with diabetic nephropathy in European Americans
    Jessica N Cooke
    Program in Molecular Medicine and Translational Science, Center for Genomics and Personalized Medicine Research, Wake Forest School of Medicine, Winston Salem, NC, USA
    Nephrol Dial Transplant 27:1505-11. 2012
    ..We tested for association of single nucleotide polymorphisms (SNPs) in MYH9 with T2DM-ESRD in European Americans; additionally, three APOL1 gene variants were evaluated...
  18. ncbi Recent advances in the understanding and management of MYH9-related inherited thrombocytopenias
    Carlo L Balduini
    Department of Internal Medicine, IRCCS Policlinico San Matteo Foundation, University of Pavia, Pavia, Italy
    Br J Haematol 154:161-74. 2011
    ..Results of a small clinical study suggested that a non-peptide thrombopoietin mimetic might greatly benefit both thrombocytopenia and bleeding tendency of MYH9-RD patients...
  19. ncbi Mts1 regulates the assembly of nonmuscle myosin-IIA
    Zhong hua Li
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 42:14258-66. 2003
    ..Altogether, these observations are consistent with mts1 regulating myosin IIA assembly by monomer sequestration and suggest that mts1 regulates cell shape and motility through the modulation of myosin-IIA function...
  20. pmc Myosin V transports secretory vesicles via a Rab GTPase cascade and interaction with the exocyst complex
    Yui Jin
    Life Sciences Institute, Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109 2216, USA
    Dev Cell 21:1156-70. 2011
    ..Moreover, these studies predict that for many pathways, molecular motors attach to vesicles prior to their formation and remain attached until fusion...
  21. pmc Secretory vesicle transport velocity in living cells depends on the myosin-V lever arm length
    Daniel H Schott
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    J Cell Biol 156:35-9. 2002
    ..Thus, secretory vesicle polarization is achieved through active transport by a myosin-V, and the motor mechanism is consistent with the lever arm model...
  22. pmc Uncoupling of expression of an intronic microRNA and its myosin host gene by exon skipping
    Matthew L Bell
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    Mol Cell Biol 30:1937-45. 2010
    ..Thus, concerted transcription and alternative splicing uncouple the level of expression of MYH7b and miR-499 when their coexpression is not required...
  23. ncbi Fibre types in skeletal muscle: a personal account
    S Schiaffino
    Department of Biomedical Sciences, University of Padova, Padova, Italy
    Acta Physiol (Oxf) 199:451-63. 2010
    ....
  24. ncbi Myosin VI: two distinct roles in endocytosis
    Tama Hasson
    Division of Biological Sciences, Section of Cell and Developmental Biology, University of California at San Diego, 2129 Bonner Hall, MC 0368, 9500 Gilman Drive, La Jolla, CA 92093 0368, USA
    J Cell Sci 116:3453-61. 2003
    ..Three distinct adapter proteins--GIPC, Dab2 and SAP97--that associate with the cargo-binding tail domain of myosin VI have been identified. These proteins may recruit myosin VI to its sites of action...
  25. pmc MYH9 is associated with nondiabetic end-stage renal disease in African Americans
    W H Linda Kao
    Department of Epidemiology, School of Medicine and Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21287, USA
    Nat Genet 40:1185-92. 2008
    ....
  26. pmc Factors controlling cardiac myosin-isoform shift during hypertrophy and heart failure
    Mahesh P Gupta
    Department of Surgery, Basic Science Division, MC5040, University of Chicago, 5841 S Maryland Avenue, Chicago, IL 60637, USA
    J Mol Cell Cardiol 43:388-403. 2007
    ..This article reviews the published data describing the role of myosin isoforms in the heart and highlighting the importance of various factors shown to influence myosin isofrom shift during physiology and disease states of the heart...
  27. pmc Elevated miR-499 levels blunt the cardiac stress response
    Joseph T C Shieh
    Gladstone Institute of Cardiovascular Disease, San Francisco, California, United States of America
    PLoS ONE 6:e19481. 2011
    ..Here we investigate miR-499, a microRNA embedded within a ventricular-specific myosin heavy chain gene, which is expressed in heart and skeletal muscle...
  28. pmc The yeast class V myosins, Myo2p and Myo4p, are nonprocessive actin-based motors
    S L Reck-Peterson
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    J Cell Biol 153:1121-6. 2001
    ..These studies suggest that if Myo2p functions in organelle transport, at least five molecules of Myo2p must be present per organelle to promote directed movement...
  29. pmc Evidence of MyomiR network regulation of beta-myosin heavy chain gene expression during skeletal muscle atrophy
    John J McCarthy
    Department of Physiology, College Health Sciences, University of Kentucky, Lexington, Kentucky 40536 0298, USA
    Physiol Genomics 39:219-26. 2009
    ..These results further expand the role of miRs in adult skeletal muscle and are consistent with a model in which the MyomiR network regulates slow myosin expression during muscle atrophy...
  30. pmc Myosin-Va transports the endoplasmic reticulum into the dendritic spines of Purkinje neurons
    Wolfgang Wagner
    Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, MD 20892, USA
    Nat Cell Biol 13:40-8. 2011
    ..Thus, we have established that an actin-based motor moves ER within animal cells, and have uncovered the mechanism for ER localization to Purkinje neuron spines, a prerequisite for synaptic plasticity...
  31. pmc Myosin VI and its binding partner optineurin are involved in secretory vesicle fusion at the plasma membrane
    Lisa M Bond
    Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB20XY, United Kingdom
    Mol Biol Cell 22:54-65. 2011
    ..These results suggest a novel role for myosin VI and optineurin in regulation of fusion pores formed between secretory vesicles and the plasma membrane during the final stages of secretion...
  32. ncbi Myosin heavy chain isoform expression following reduced neuromuscular activity: potential regulatory mechanisms
    R J Talmadge
    Department of Human Nutrition, Foods and Exercise, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061 0430, USA
    Muscle Nerve 23:661-79. 2000
    ....
  33. ncbi Myosin VI rewrites the rules for myosin motors
    H Lee Sweeney
    Department of Physiology, University of Pennsylvania School of Medicine, B700 Richards Building, 3700 Hamilton Walk, Philadelphia, PA 19104 6085, USA
    Cell 141:573-82. 2010
    ..Given that other classes of myosins may share some of these features, understanding the design principles of myosin VI will help guide the study of the functions of myosins that adopt similar strategies...
  34. pmc Structural basis for myosin V discrimination between distinct cargoes
    Natasha Pashkova
    Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
    EMBO J 25:693-700. 2006
    ....
  35. pmc Binding of internalized receptors to the PDZ domain of GIPC/synectin recruits myosin VI to endocytic vesicles
    Samia N Naccache
    Section of Cell and Developmental Biology, Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 103:12735-40. 2006
    ....
  36. ncbi Sox6 is required for normal fiber type differentiation of fetal skeletal muscle in mice
    Nobuko Hagiwara
    University of California, Davis, Division of Cardiovascular Medicine Rowe Program in Human Genetics, Davis, California 95616, USA
    Dev Dyn 236:2062-76. 2007
    ..Together, our results suggest that Sox6 plays a critical role in the fiber type differentiation of fetal skeletal muscle...
  37. pmc Myo6 facilitates the translocation of endocytic vesicles from cell peripheries
    Laura Aschenbrenner
    Section of Cell and Developmental Biology, Division of Biological Sciences, University of California at San Diego, La Jolla, California 92093, USA
    Mol Biol Cell 14:2728-43. 2003
    ....
  38. ncbi Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport
    Mitsunori Fukuda
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 277:12432-6. 2002
    ..The discovery that myosin Va indirectly recognizes its cargo through Slac2-a, a novel Rab27A/B effector, should shed light on molecular recognition of its specific cargo by class V myosin...
  39. ncbi Mutation in myosin heavy chain 6 causes atrial septal defect
    Yung Hao Ching
    Institute of Genetics, University of Nottingham, Queen s Medical Centre, Nottingham NG7 2UH, UK
    Nat Genet 37:423-8. 2005
    ..These data provide evidence for a link between a transcription factor, a structural protein and congenital heart disease...
  40. ncbi Mutations in sarcomere protein genes in left ventricular noncompaction
    Sabine Klaassen
    Max Delbruck Center for Molecular Medicine, Robert Roessle Strasse 10, 13092 Berlin, Germany
    Circulation 117:2893-901. 2008
    ..We speculated that mutations in sarcomere protein genes known to cause hypertrophic cardiomyopathy and dilated cardiomyopathy may be associated with left ventricular noncompaction...
  41. pmc African ancestry allelic variation at the MYH9 gene contributes to increased susceptibility to non-diabetic end-stage kidney disease in Hispanic Americans
    Doron M Behar
    Molecular Medicine Laboratory, Rambam Health Care Campus, Haifa 31096, Israel
    Hum Mol Genet 19:1816-27. 2010
    ....
  42. pmc The MYH9/APOL1 region and chronic kidney disease in European-Americans
    Conall M O'Seaghdha
    National Heart, Lung and Blood Institute s Framingham Heart Study and Center for Population Studies, Framingham, MA, USA
    Hum Mol Genet 20:2450-6. 2011
    ..These data suggest that the MYH9 polymorphism rs4821480 is associated with an increased risk of non-diabetic CKD in individuals of European ancestry...
  43. ncbi Expression of multiple slow myosin heavy chain genes reveals a diversity of zebrafish slow twitch muscle fibres with differing requirements for Hedgehog and Prdm1 activity
    Stone Elworthy
    MRC Centre for Developmental and Biomedical Genetics and Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Development 135:2115-26. 2008
    ..The adult trunk slow fibres express smyhc2 and smyhc3, but lack smyhc1 expression. The different slow fibres in the craniofacial muscles variously express smyhc1, smyhc2 and smyhc3, and all differentiate independently of Prdm1...
  44. ncbi Tropomyosin-containing actin cables direct the Myo2p-dependent polarized delivery of secretory vesicles in budding yeast
    D W Pruyne
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA
    J Cell Biol 143:1931-45. 1998
    ..Therefore, actin cables respond to polarity cues independently of the overall distribution of cortical patches and are able to directly target the Myo2p-dependent delivery of secretory vesicles and polarization of growth...
  45. ncbi Spatial coordination of cytokinetic events by compartmentalization of the cell cortex
    Jeroen Dobbelaere
    Institute of Biochemistry, Swiss Federal Institute of Technology, ETH Honggerberg, 8093 Zurich, Switzerland
    Science 305:393-6. 2004
    ..In turn, such factors were required for actomyosin ring function and membrane abscission. Thus, a specialized cortical compartment ensures the spatial coordination of cytokinetic events...
  46. pmc Cardiac hypertrophy and thyroid hormone signaling
    Wolfgang Dillmann
    University of California, San Diego, USA
    Heart Fail Rev 15:125-32. 2010
    ..The failing heart may develop a "hypothyroid" status contributing to diminished cardiac contractile function...
  47. ncbi The mouse Snell's waltzer deafness gene encodes an unconventional myosin required for structural integrity of inner ear hair cells
    K B Avraham
    Mammalian Genetics Laboratory, NCI Frederick Cancer Research and Development Center, Maryland 21702, USA
    Nat Genet 11:369-75. 1995
    ..The requirement for myosin VI in hearing makes this gene an excellent candidate for a human deafness disorder...
  48. ncbi Position of nonmuscle myosin heavy chain IIA (NMMHC-IIA) mutations predicts the natural history of MYH9-related disease
    Alessandro Pecci
    Department of Internal Medicine, University of Pavia and Istituto di Ricovero e Cura a Carattere Scientifico IRCCS, Policlinico San Matteo Foundation, Pavia, Italy
    Hum Mutat 29:409-17. 2008
    ..These findings are relevant not only to patients' clinical management but also to the elucidation of the pathogenesis of the disease...
  49. ncbi The role of myosin V in exocytosis and synaptic plasticity
    Rudiger Rudolf
    Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Eggenstein Leopoldshafen, Germany
    J Neurochem 116:177-91. 2011
    ..Myosin V is thus discussed as an important modulator of synaptic plasticity...
  50. pmc Mutations of MYO6 are associated with recessive deafness, DFNB37
    Zubair M Ahmed
    Section on Human Genetics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Rockville, MD 20850, USA
    Am J Hum Genet 72:1315-22. 2003
    ....
  51. ncbi Alpha-cardiac myosin heavy chain (MYH6) mutations affecting myofibril formation are associated with congenital heart defects
    Javier T Granados-Riveron
    Institute of Genetics, School of Biology, Queen s Medical Centre, University of Nottingham, Nottingham, UK
    Hum Mol Genet 19:4007-16. 2010
    ..Our data indicate that functional variants of MYH6 are associated with cardiac malformations in addition to ASD and provide a novel potential mechanism. Such phenotypic heterogeneity has been observed in other genes mutated in CHD...
  52. pmc Ablation of nonmuscle myosin II-B and II-C reveals a role for nonmuscle myosin II in cardiac myocyte karyokinesis
    Xuefei Ma
    Laboratory of Molecular Cardiology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1583, USA
    Mol Biol Cell 21:3952-62. 2010
    ..Our study shows that NM II is involved in regulating cardiac myocyte karyokinesis by affecting microtubule dynamics...
  53. pmc Myo4p is a monomeric myosin with motility uniquely adapted to transport mRNA
    Brian D Dunn
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA
    J Cell Biol 178:1193-206. 2007
    ..Our results suggest that the oligomeric state of Myo4p is important for its motility and ability to transport mRNA...
  54. pmc MYH9 is a major-effect risk gene for focal segmental glomerulosclerosis
    Jeffrey B Kopp
    Kidney Disease Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Genet 40:1175-84. 2008
    ..2, 95% CI = 1.5-3.4; n = 433), but not type 2 diabetic ESKD (n = 476). Genetic variation at the MYH9 locus substantially explains the increased burden of FSGS and hypertensive ESKD among African Americans...
  55. ncbi Molecular regulation of individual skeletal muscle fibre types
    E E Spangenburg
    Department of Biomedical Sciences and Department of Medical Pharmacology and Physiology, Dalton Cardiovascular Institute, University of Missouri, Columbia, MO 65211, USA
    Acta Physiol Scand 178:413-24. 2003
    ....
  56. ncbi Regulation of chamber-specific gene expression in the developing heart by Irx4
    Z Z Bao
    Howard Hughes Medical Institute and Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Science 283:1161-4. 1999
    ..Thus, Irx4 may play a critical role in establishing chamber-specific gene expression in the developing heart...
  57. ncbi Human cardiac myosin heavy chain isoforms in fetal and failing adult atria and ventricles
    P J Reiser
    Department of Oral Biology, Ohio State University, Columbus 43210, Ohio, USA
    Am J Physiol Heart Circ Physiol 280:H1814-20. 2001
    ..Furthermore, the marked alteration in atrial MHC isoform composition, associated with cardiomyopathy, does not represent a regression to a pattern that is uniquely characteristic of the fetal stage...
  58. ncbi Mutations in MYH9 result in the May-Hegglin anomaly, and Fechtner and Sebastian syndromes. The May-Heggllin/Fechtner Syndrome Consortium
    M Seri
    Laboratory of Molecular Genetics, Institute G Gaslini, Genoa, Italy
    Nat Genet 26:103-5. 2000
    ..Thus, our results suggest that mutations in MYH9 result in three megakaryocyte/platelet/leukocyte syndromes and are important in the pathogenesis of sensorineural deafness, cataracts and nephritis...
  59. ncbi Immunofluorescence analysis of neutrophil nonmuscle myosin heavy chain-A in MYH9 disorders: association of subcellular localization with MYH9 mutations
    Shinji Kunishima
    Japanese Red Cross Aichi Blood Center, Seto, Japan
    Lab Invest 83:115-22. 2003
    ..Immunofluorescence analysis of neutrophil NMMHCA is useful as a screening test for the clear hematopathologic classification of MYH9 disorders...
  60. pmc Myosin VI contributes to synaptic transmission and development at the Drosophila neuromuscular junction
    Marta Kisiel
    Department of Biology, University of Toronto Mississauga, 3359 Mississauga Rd, Mississauga, ON L5L1C6, Canada
    BMC Neurosci 12:65. 2011
    ..We previously recovered jar in a screen for genes that modify neuromuscular junction (NMJ) development and here we report on the genetic analysis of Myosin VI in synaptic development and function using loss of function jar alleles...
  61. ncbi Myofibrillar myopathy caused by a mutation in the motor domain of mouse MyHC IIb
    Ramakrishna Kurapati
    MRC Mammalian Genetics Unit, Harwell OX11 0RD, UK
    Hum Mol Genet 21:1706-24. 2012
    ..Recapitulation of the L342Q change in the functional equivalent of mouse MYH4 in human muscles, MYH1, results in a more aggregate-prone protein...
  62. pmc Coding sequence rare variants identified in MYBPC3, MYH6, TPM1, TNNC1, and TNNI3 from 312 patients with familial or idiopathic dilated cardiomyopathy
    Ray E Hershberger
    University of Miami Miller School of Medicine, Fla 33136, USA
    Circ Cardiovasc Genet 3:155-61. 2010
    ..We have previously resequenced 9 genes in a cohort of idiopathic or familial DCM probands for rare variants, and now we report resequencing results for 5 more genes with established relationships to DCM...
  63. pmc Single-molecule reconstitution of mRNA transport by a class V myosin
    Thomas E Sladewski
    Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, Vermont, USA
    Nat Struct Mol Biol 20:952-7. 2013
    ..Building the complexity of single-molecule in vitro assays is necessary to understand how these complexes function within cells...
  64. ncbi Impact of beta-myosin heavy chain isoform expression on cross-bridge cycling kinetics
    Veronica L M Rundell
    Center for Cardiovascular Research, Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, Illinois 60612, USA
    Am J Physiol Heart Circ Physiol 288:H896-903. 2005
    ..05 (ANOVA)] Thus cross-bridge cycling, under high strain, for alpha-MHC is three times higher than for beta-MHC. Furthermore, under isometric conditions, alpha-MHC and beta-MHC cross bridges hydrolyze ATP independently of one another...
  65. pmc Notochord induction of zebrafish slow muscle mediated by Sonic hedgehog
    C S Blagden
    Developmental Biology Research Centre, The Randall Institute, King s College London, UK
    Genes Dev 11:2163-75. 1997
    ..We suggest that Shh acts to induce myoblasts committed to slow muscle differentiation from uncommitted presomitic mesoderm...
  66. pmc Immobile myosin-II plays a scaffolding role during cytokinesis in budding yeast
    Carsten Wloka
    Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA
    J Cell Biol 200:271-86. 2013
    ..displayed Myo1-dependent immobility during cytokinesis, suggesting that Myo1 plays a scaffolding role in the assembly of a cytokinesis machine...
  67. pmc Molecular consequences of the R453C hypertrophic cardiomyopathy mutation on human β-cardiac myosin motor function
    Ruth F Sommese
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 110:12607-12. 2013
    ..Loaded in vitro motility assay confirms that the net force in the ensemble is indeed increased. Overall, this study suggests that the R453C mutation should result in a hypercontractile state in the heart muscle. ..
  68. ncbi Developmental expression and cardiac transcriptional regulation of Myh7b, a third myosin heavy chain in the vertebrate heart
    Andrew S Warkman
    Department of Cellular and Molecular Medicine, University of Arizona Health Sciences Center, Tucson, Arizona 85724, USA
    Cytoskeleton (Hoboken) 69:324-35. 2012
    ..Finally, the Myh7b gene shows a transcriptional response similar to Myh6 in response to cardiac hypertrophy...
  69. ncbi Polymorphisms in the nonmuscle myosin heavy chain 9 gene (MYH9) are associated with the progression of IgA nephropathy in Chinese
    Wenrong Cheng
    Renal Division, Peking University First Hospital, Peking University Institute of Nephrology, and Key Laboratory of Renal Disease, Ministry of Health of China, Beijing 100034, People s Republic of China
    Nephrol Dial Transplant 26:2544-9. 2011
    ..Thus, in the present study, we aim to clarify whether MYH9 confers a shared mechanism among different causes of ESRD and to seek possible further insight into our understanding of IgAN by applying GWAS data from ESRD to IgAN...
  70. ncbi Fast skeletal muscle myosin heavy chain gene cluster of medaka Oryzias latipes enrolled in temperature adaptation
    Chun Shi Liang
    Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    Physiol Genomics 29:201-14. 2007
    ....
  71. ncbi Changes in myosin heavy chain mRNA and protein expression in human skeletal muscle with age and endurance exercise training
    Kevin R Short
    Division of Endocrinology, Department of Internal Medicine, Mayo Clinic School of Medicine, 200 First St SW, Rochester, MN 55905, USA
    J Appl Physiol 99:95-102. 2005
    ..In response to endurance exercise, regulation of MHC isoform transcripts remains robust in older muscle, but this did not result in corresponding changes in MHC protein expression...
  72. pmc Comparative kinetic and functional characterization of the motor domains of human nonmuscle myosin-2C isoforms
    Sarah M Heissler
    Institute for Biophysical Chemistry, Hannover Medical School, 30625 Hannover, Germany
    J Biol Chem 286:21191-202. 2011
    ..The greatest responsiveness of the NMHC-2C isoforms is observed in the physiological range from 0.2 to 1.5 mM free Mg(2+) ions, affecting their duty ratio, velocity, and tension-bearing properties...
  73. ncbi Skeletal muscle function and fibre types: the relationship between occlusal function and the phenotype of jaw-closing muscles in human
    J J Sciote
    Department of Orthodontics and Dentofacial Orthopedics, School of Dental Medicine, University of Pittsburgh, Salk Hall 3501 Terrace St, Pittsburgh PA 15261 1932, USA
    J Orthod 27:15-30. 2000
    ..Healthy adult human limb skeletal muscle contains type I, IIA, IIB, and IIX myosin heavy chains. The jaw-closing muscles of most carnivores and primates have tissue-specific expression of the type IIM or ..
  74. pmc Knockdown of embryonic myosin heavy chain reveals an essential role in the morphology and function of the developing heart
    Catrin Sian Rutland
    School of Biomedical Sciences, University of Nottingham, Queens Medical Centre, Derby Road, Nottingham, NG7 2UH, UK
    Development 138:3955-66. 2011
    ..This is despite the knowledge that other structural proteins, such as alpha and beta myosin heavy chains and cardiac alpha actin, play crucial roles in atrial septal development and cardiac function...
  75. pmc MYH9 and APOL1 are both associated with sickle cell disease nephropathy
    Allison E Ashley-Koch
    Center for Human Genetics, Duke University Medical Center, Durham, NC 27710, USA allison ashleykoch duke edu
    Br J Haematol 155:386-94. 2011
    ..Our data provide insight into the pathobiology of renal dysfunction in SCD, suggesting that MYH9 and APOL1 are both associated with risk...
  76. ncbi Switch between large hand-over-hand and small inchworm-like steps in myosin VI
    So Nishikawa
    Graduate School of Frontier Biosciences, Osaka University, Yamadaoka, Suita, Osaka 565 0871, Japan
    Cell 142:879-88. 2010
    ..Switching between these two mechanisms in a strain-sensitive, ADP-dependent manner allows myosin VI to fulfill its multiple cellular tasks including vesicle transport and membrane anchoring...
  77. pmc Two distinct myosin light chain structures are induced by specific variations within the bound IQ motifs-functional implications
    Mohammed Terrak
    Boston Biomedical Research Institute, 64 Grove Street, Watertown, MA 02472, USA
    EMBO J 22:362-71. 2003
    ..The resulting 'free' N-lobes of myosin light chains in the extended conformation could mediate the formation of ternary complexes during protein localization and/or partner recruitment...
  78. ncbi Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA
    R M Long
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Science 277:383-7. 1997
    ..A cis-acting element in the 3'-untranslated region of ASH1 mRNA is sufficient to localize a chimeric RNA to daughter cells. These results suggest that localization of mRNA may have been an early property of the eukaryotic lineage...
  79. ncbi Leading edge-secreted Dpp cooperates with ACK-dependent signaling from the amnioserosa to regulate myosin levels during dorsal closure
    Baharak Zahedi
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada
    Dev Dyn 237:2936-46. 2008
    ..ACK function in the amnioserosa may generate a signal cooperating with Dpp secreted from the epidermis in driving zip expression in these two tissues, ensuring that cell shape changes in dorsal closure occur in a coordinated manner...
  80. ncbi Mother cell-specific HO expression in budding yeast depends on the unconventional myosin myo4p and other cytoplasmic proteins
    R P Jansen
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 84:687-97. 1996
    ..In an accompanying paper, we show that SHE genes are needed for the accumulation in daughter nuclei of Ash1p, a repressor of HO...
  81. ncbi Advances in the understanding of MYH9 disorders
    Shinji Kunishima
    Department of Advanced Diagnosis, Clinical Research Center, National Hospital Organization Nagoya Medical Center, Nagoya, Japan
    Curr Opin Hematol 17:405-10. 2010
    ..The present review summarizes the recent advances in genetic diagnosis and our understanding of the pathogenetic mechanisms of MYH9 mutations and the development of nonhematological complications...
  82. ncbi A protein-protein interaction of stress-responsive myosin VI endowed to inhibit neural progenitor self-replication with RNA binding protein, TLS, in murine hippocampus
    Takeshi Takarada
    Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences, Kanazawa University Graduate School of Natural Science and Technology, Kakuma machi, Kanazawa, Ishikawa 920 1192, Japan
    J Neurochem 110:1457-68. 2009
    ..These results suggest that Myo6 may play a pivotal role in the mechanism underlying the suppressed adult neurogenesis after traumatic stress in association with TLS...
  83. ncbi Training affects myosin heavy chain phenotype in the trapezius muscle of women
    F Kadi
    Department of Anatomy, Umea University, Sweden
    Histochem Cell Biol 112:73-8. 1999
    ..The presence of small-sized muscle fibres expressing the developmental isoforms of MyHC suggests that strength and endurance training induced the formation of new muscle fibres...
  84. ncbi Myopathies associated with myosin heavy chain mutations
    A Oldfors
    Department of Pathology, Sahlgrenska University Hospital, Goteborg, Sweden
    Acta Myol 23:90-6. 2004
    ..These patients showed slowly progressive muscle weakness but no overt cardiomyopathy. These two muscle diseases, which are caused by mutations in MyHC, form the basis of a novel entity: "Myosin myopathies"...
  85. ncbi Role of myosin VI in the differentiation of cochlear hair cells
    T Self
    MRC Institute of Hearing Research, University Park, Nottingham, NG7 2RD, United Kingdom
    Dev Biol 214:331-41. 1999
    ....
  86. ncbi Hypertrophic cardiomyopathy: distribution of disease genes, spectrum of mutations, and implications for a molecular diagnosis strategy
    Pascale Richard
    UF de Cardiogénétique et Myogénétique, Service de Biochimie B, Hopital de la Salpetriere, 47 Bld de l Hopital, 75651 Paris Cedex 13, France
    Circulation 107:2227-32. 2003
    ..The aim of the present study was to perform a systematic screening of these genes in a large population, to evaluate the distribution of the disease genes, and to determine the best molecular strategy in clinical practice...
  87. pmc Myosin Vc is a molecular motor that functions in secretory granule trafficking
    Damon T Jacobs
    Department of Cell and Molecular Physiology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Biol Cell 20:4471-88. 2009
    ..These results provide the first live-cell imaging of Myo5c and indicate that Myo5c functions in secretory granule trafficking...
  88. ncbi Fiber type composition of four hindlimb muscles of adult Fisher 344 rats
    R S Staron
    Department of Biomedical Sciences, College of Osteopathic Medicine, Ohio University, Athens 45701, USA
    Histochem Cell Biol 111:117-23. 1999
    ..5+/-2.8%) and IIDB (5.2+/-2.3%). In conclusion, hybrid fibers make up a considerable portion of normal rat limb musculature and are an important population that should not be ignored...
  89. pmc Loss of myosin VI no insert isoform (NoI) induces a defect in clathrin-mediated endocytosis and leads to caveolar endocytosis of transferrin receptor
    Claudia Puri
    Cambridge Institute for Medical Research, University of Cambridge, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 284:34998-5014. 2009
    ..Moreover the clathrin adaptor protein, AP-2, necessary for TfR internalization, follows the receptor and relocalizes in caveolae in Snell's waltzer fibroblasts...
  90. ncbi Sarcomere protein gene mutations in hypertrophic cardiomyopathy of the elderly
    Hideshi Niimura
    Howard Hughes Medical Institute and Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Circulation 105:446-51. 2002
    ..Hypertrophic cardiomyopathy of the elderly has similar clinical features but, notably, a later age of onset and noncontributory family history. Causes of elderly-onset hypertrophic cardiomyopathy are unknown...
  91. ncbi Thermal preconditioning prevents fiber type transformation of the unloading induced-atrophied muscle in rats
    Isao Takeda
    Department of Physical Therapy, Himeji Dokkyo University, Himeji, Japan
    J Muscle Res Cell Motil 30:145-52. 2009
    ..Therefore, thermal preconditioning provides a new approach to prevent disuse-induced fiber type transformation of skeletal muscle...
  92. ncbi Decreased expression of myogenic transcription factors and myosin heavy chains in Caenorhabditis elegans muscles developed during spaceflight
    Akira Higashibata
    Japan Aerospace Exploration Agency, 2 1 1, Sengen, Tsukuba, Ibaraki 305 8505, Japan
    J Exp Biol 209:3209-18. 2006
    ....
  93. pmc Myo2p, a class V myosin in budding yeast, associates with a large ribonucleic acid-protein complex that contains mRNAs and subunits of the RNA-processing body
    Wakam Chang
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
    RNA 14:491-502. 2008
    ..P-body disassembly is delayed in the motor mutant, myo2-66, indicating that Myo2p may facilitate the release of mRNAs from the P-body...
  94. pmc The structure of the Myo4p globular tail and its function in ASH1 mRNA localization
    Alexander Heuck
    Institute of Structural Biology, Helmholtz Zentrum Munchen, German Research Center for Environmental Health, Munich, Germany
    J Cell Biol 189:497-510. 2010
    ..The structure shows pronounced similarities to membrane-tethering complexes and indicates that Myo4p may not undergo auto-inhibition of its motor domain...
  95. ncbi Human embryonic stem cells develop into multiple types of cardiac myocytes: action potential characterization
    Jia Qiang He
    Department of Anatomy, University of Wisconsin, Madison, Wis 53792 3248, USA
    Circ Res 93:32-9. 2003
    ..Thus, hES provide a renewable source of distinct types of human cardiac myocytes for basic research, pharmacological testing, and potentially therapeutic applications...
  96. ncbi Asymmetric loading of Kar9 onto spindle poles and microtubules ensures proper spindle alignment
    Dimitris Liakopoulos
    Institute of Biochemistry, Swiss Federal Institute of Technology, ETH Hoenggerberg, HPM, CH 8093 Zurich, Switzerland
    Cell 112:561-74. 2003
    ..In turn, asymmetric distribution of Kar9 ensures that only one pole orients toward the bud. Our results indicate that Cdk1-dependent spindle asymmetry ensures proper alignment of the mitotic spindle with the cell division axis...
  97. pmc Coiled-coil-mediated dimerization is not required for myosin VI to stabilize actin during spermatid individualization in Drosophila melanogaster
    Tatsuhiko Noguchi
    Center for Developmental Biology, RIKEN, Kobe, Japan
    Mol Biol Cell 20:358-67. 2009
    ..We conclude that myosin VI does not need to dimerize via the predicted coiled coil to stabilize actin in vivo...
  98. ncbi Kinetic mechanism and regulation of myosin VI
    E M De La Cruz
    Department of Physiology, Pennsylvania Muscle Institute, University of Pennsylvania School of Medicine, 3700 Hamilton Walk, Philadelphia, Pennsylvania 19104-6085, USA
    J Biol Chem 276:32373-81. 2001
    ..We propose an alternating site model for the stepping and processivity of two-headed high duty ratio myosins...
  99. ncbi Hereditary myosin myopathies
    Anders Oldfors
    Department of Pathology, Sahlgrenska University Hospital, S 413 45 Goteborg, Sweden
    Neuromuscul Disord 17:355-67. 2007
    ..Clinical findings, muscle morphology and molecular genetics in hereditary myosin myopathies are summarized in this review...
  100. ncbi Loaded shortening and power output in cardiac myocytes are dependent on myosin heavy chain isoform expression
    T J Herron
    Department of Physiology, University of Missouri School of Medicine, Columbia, Missouri 65212, USA
    Am J Physiol Heart Circ Physiol 281:H1217-22. 2001
    ..These results indicate that MHC expression is a primary determinant of the shape of force-velocity relationships, velocity of loaded shortening, and overall power output-generating capacity of individual cardiac myocytes...
  101. ncbi The apolipoprotein L1 (APOL1) gene and nondiabetic nephropathy in African Americans
    Barry I Freedman
    Section on Nephrology, Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, NC 27157 1053, USA
    J Am Soc Nephrol 21:1422-6. 2010
    ..This association likely explains racial disparities in nondiabetic nephropathy as a result of the high prevalence of risk alleles in individuals of African ancestry...

Research Grants64

  1. Cellular and Molecular Regulation of Thrombopoiesis
    Ramesh A Shivdasani; Fiscal Year: 2012
    ..Characterizing key molecular pathways of platelet formation should lead to better control of circulating platelet counts and to implementation of less toxic treatments for cancer. ..
  2. Impact of AMP-activated kinase on sex differences in hypertrophic cardiomyopathy
    John P Konhilas; Fiscal Year: 2013
    ..Therefore, it is of major clinical significance that the mechanistic link be determined between cardiac disease, sex/gender differences and energetic regulators in the heart. ) ..
  3. Autoimmunity in the Mediation of Infectious Myocarditis
    Jay Reddy; Fiscal Year: 2013
    ..These studies may then create opportunities to derive immunotherapies in the form of altered peptide ligands and tolerogens as antigen-specific treatment modalities, including personalized therapies, for DCM patients.] ..
  4. NFATc3 in chronic hypoxic pulmonary hypertension
    Laura V Gonzalez Bosc; Fiscal Year: 2012
    ..A better understanding of this mechanisms in PAH will lead to the development of novel therapeutic approaches to prevent and cure this debilitating disease. ..
  5. Silencing cardiac-specific microRNAs to prevent pathological remodeling in conges
    MATTHEW SWAN LAWRENCE; Fiscal Year: 2011
    ..In addition, we will comprehensively characterize miR-208-relevant pathophysiology in a monkey model of congestive heart failure to allow critical pre-clinical efficacy studies to be completed in phase II. ..
  6. Cellular Mechanisms of Lymphatic Muscle Contractility
    MICHAEL JOHN DAVIS; Fiscal Year: 2012
    ..These studies will investigate which proteins allow these vessels to contract so that specific therapeutic agents can be developed to correct lymphatic pump dysfunction and drainage of edematous tissues. ..
  7. Impact of AMP-activated kinase on sex differences in hypertrophic cardiomyopathy
    John P Konhilas; Fiscal Year: 2013
    ..Therefore, it is of major clinical significance that the mechanistic link be determined between cardiac disease, sex/gender differences and energetic regulators in the heart. ) ..
  8. Development of Inhibitors of the Leukemia Fusion Protein CBFbeta-SMMHC
    Roger A Rajewski; Fiscal Year: 2013
    ..These inhibitors also have the potential to be less toxic than standard chemotherapy, resulting in reduced side effects from treatment. ..
  9. Rho Kinase and Airway Hyperresponsiveness
    Stephanie A Shore; Fiscal Year: 2013
    ..Thus, the ROCK pathway may be an important and still relatively unexplored therapeutic target in asthma. Understanding the role of ROCKs in mouse models of asthma could lead to new strategies for preventing this disease. ..
  10. Functional Consequences of FHC-linked RLC Mutations.
    Danuta Szczesna-Cordary; Fiscal Year: 2012
    ....
  11. Cellular and Biochemical Studies of Myosin Assembly in Dictyostelium
    Thomas Egelhoff; Fiscal Year: 2009
    ..The studies we propose addressing the full complement of Dictyostelium alpha kinases will also provide a powerful platform for comparisons and insights into possible roles of alpha kinases in mammalian systems. ..
  12. Calcium-dependent Regulation of Smooth Muscle Phenotype
    BRIAN WAMHOFF; Fiscal Year: 2010
    ....
  13. NFAT and MEF-2 Choreograph Bladder Wall Remodeling Following Partial Outlet Obstr
    STEPHEN ANTHONY ZDERIC; Fiscal Year: 2010
    ..New therapy to preserve and enhance native bladder function will help avoid the development of the end stage bladder. ..
  14. A TG model for functional effects of FHC mutations in b-cardiac myosin
    Susan Lowey; Fiscal Year: 2013
    ..These studies should serve to increase our knowledge of the basic molecular mechanism of this disease by examining the effects of an FHC mutation at increasing levels of complexity from the molecule to the filament. ..
  15. Botulinum toxin in muscles of mastication
    Susan W Herring; Fiscal Year: 2012
    ..The results will guide clinicians in understanding functional consequences and help to validate clinical rationales for treatment. ..
  16. Chromatin mechanism of molecular motor gene regulation
    Ching Pin Chang; Fiscal Year: 2013
    ....
  17. Reactive Oxygen Species in Coronary Collateral Growth
    William M Chilian; Fiscal Year: 2013
    ..These studies will provide insight into the cell-specific locations where ROS and redox signaling modulate coronary collateral growth. ..
  18. Molecular Imaging of Stem Cell Transplantation in the Heart
    Juri G Gelovani; Fiscal Year: 2010
    ..This approach will provide insights into the fate of human stem cell transplantation into the heart. ..
  19. Identifying the earliest events in HIV-1 associated nephropathy via genome-wide
    Ali G Gharavi; Fiscal Year: 2013
    ..Identification of molecular mediators of HIVAN is expected to provide significant insight into the pathogenesis of disease and provide novel targets for therapeutic intervention. ..
  20. Molecular basis of age-dependent changes in airway smooth muscle functions
    Deepak A Deshpande; Fiscal Year: 2013
    ..Our findings may help develop tools to improve respiratory functions, and modify diagnostic and treatment regimens for obstructive pulmonary diseases in the elderly. ..
  21. Mechanistic basis and potential therapies for myosin storage myopathy
    Sanford I Bernstein; Fiscal Year: 2013
    ..This multifaceted approach will provide novel insights into the developmental and biophysical bases of MSM and yield potential therapeutic approaches that may be useful for treating MSM and other inclusion body diseases. ..
  22. In Situ Sensing of Single Myosin Function in Hypertrophy Disease
    Thomas P Burghardt; Fiscal Year: 2013
    ..RLC crystallization and structure determination will investigate the structural basis of RLC regulation of myosin as well as the impact of FHC-linked mutations on RLC structure. ..
  23. ANATOMICAL SPECIALIZATIONS OF THE HUMAN PHARYNX
    Liancai Mu; Fiscal Year: 2012
    ..The data are critical for a better understanding of the pathophysiological mechanisms of dysphagia in PD and for the development of novel therapies to treat this life-threatening disorder. ..
  24. An In Vitro Model of Stem Cell Innervation of Myotubes
    James J Hickman; Fiscal Year: 2013
    ....
  25. Cerebrovascular Myosin Light Chain Phosphorylation in Fetus, Newborn, and Adult
    William J Pearce; Fiscal Year: 2013
    ....
  26. Cellular Regulation of Dictyostelium Myosin Heavy Chain Kinases
    Paul A Steimle; Fiscal Year: 2012
    ....
  27. Characterizing early neural crest phosphoregulation using antiphosphatase targets
    Laura S Gammill; Fiscal Year: 2013
    ..Understanding neural crest development in its normal, embryonic context will allow us to decipher the abnormal function and activation of neural crest pathways in disease. ..
  28. MECHANISM OF MUSCLE WASTING IN AGING MAN
    K Sreekumaran Nair; Fiscal Year: 2013
    ..The proposed studies will apply novel and innovative methodologies developed in our laboratory to address important mechanistic questions that may reveal potential therapeutic targets to combat sarcopenia. ..
  29. Role of SIRT3 in alcoholic heart muscle disease
    Charles H Lang; Fiscal Year: 2013
    ..thereby providing a broad translational basis for our research focus and the potential for therapeutic development. ..
  30. Myosin Isoforms in Urinary Bladder Function
    Muthu Periasamy; Fiscal Year: 2010
    ..This proposal will examine the role of SM2 myosin isoform in bladder development and function using a mouse model which does not contain this protein. ..
  31. Spectroscopy of myosin light chain domain dynamics
    Osha Roopnarine; Fiscal Year: 2010
    ....
  32. Myogenic Cell Lineages and Muscle Fiber Type Formation
    JOSEPH XAVIER DIMARIO; Fiscal Year: 2013
    ..These studies will provide important insight into the molecular and cellular regulation of muscle phenotype during development and regeneration from disease states. ..
  33. Identifying colon cancer modifier genes with zebrafish
    Michael A Pack; Fiscal Year: 2010
    ..Functional assessment of these genes in mlt mutants provides a rapid, in vivo assay of their suitability for pharmacological targeting. ..
  34. Redox sensitive factors that regulate tissue architecture in the intestine
    Michael A Pack; Fiscal Year: 2012
    ..This work will thus enhance our understanding of mechanisms regulating homeostatic and injury induced tissue regeneration in the intestine, as well how intestinal cancers undergo invasive transformation. ..
  35. RNA profiling of HIV-associated nephropathy in patients with the MYH9 risk allele
    PAUL EVAN KLOTMAN; Fiscal Year: 2010
    ..Findings from the proposed research are likely to provide insights into the increased susceptibility to kidney disease and end-stage renal disease among people of African descent. ..
  36. Genetic Determinants of Susceptibility to Kidney Disease in African Americans
    Thomas M Coffman; Fiscal Year: 2010
    ..These animal models will be powerful tools for identifying new strategies for preventing ESRD in African Americans. ..
  37. MicroRNA Control of Cardiac Gene Expression, Function and Disease
    Eric N Olson; Fiscal Year: 2013
    ..Ultimately, we hope to exploit our understanding of miRNA biology to uncover new disease mechanisms and therapeutic approaches for muscle disease. ..
  38. Microenvironmental Endogenous Reprogramming of Differentiated Smooth Muscle Cells
    MARY CM WEISER-EVANS; Fiscal Year: 2013
    ..For Aim Two, we will determine the fate of SMC-derived VPCs in response to wire-induced vascular injury. ..
  39. Myosin Isoforms in Relation to Function in Human Heart
    Richard L Moss; Fiscal Year: 2010
    ..other mammals, the maximum work capacity of myocardium in health and disease is limited by the isoforms of myosin heavy chains (MHC) expressed in the left ventricle-there is a nearly 10- fold range in actin-activated ATPase activity ..
  40. Actomyosin-Based Podocyte Contractility and Glomerular Pathobiology
    Joel M Henderson; Fiscal Year: 2012
    ..This work will serve as an important step in the effort to reduce the toll of end stage renal disease on the American public. ..
  41. Stem Cells in the Developing Heart
    Marcello Rota; Fiscal Year: 2013
    ..abstract_text> ..
  42. Role of the Inflammatory Cytokine Oncostatin-M in Promoting Atherosclerosis
    Errol Wijelath; Fiscal Year: 2013
    ..This proposed work will elucidate the role of an important biological factor, OSM, in the evolution of the atherosclerosis. It will also identify STAT-1 pathway as a therapeutic target for controlling pathological vascular remodeling. ..
  43. Single Skeletal Muscle Fiber Mechanics and Myosin Kinetics in Human Aging
    MARK STUART MILLER; Fiscal Year: 2012
    ....
  44. Molecular Characterization of Cardiomyopathy Mutations in Human Cardiac Myosin
    JAMES ANTHONY SPUDICH; Fiscal Year: 2013
    ....
  45. The role of autoimmune natural IgM in human myocardial infarction
    Ming Zhang; Fiscal Year: 2010
    ..PUBLIC HEALTH RELEVANCE This study will provide important insight of a new mechanism in ischemic myocardial injury, and may identify new markers for myocardial infarction. ..
  46. MOTOR FUNCTION OF CARDIAC B MYOSIN MUTANTS
    Mitsuo Ikebe; Fiscal Year: 1999
    ....
  47. STRUCTURE/FUNCTION OF MYOSIN IN SKELETAL MUSCLE
    Gordon Lutz; Fiscal Year: 2001
    ....
  48. MYOBLAST GROWTH AND DIFFERENTIATION DURING MUSCLE AGING
    Charlotte Peterson; Fiscal Year: 2000
    ..The feasibility of using IGFs as therapeutic agents to maintain muscle mass in the elderly can then be evaluated. ..
  49. ALTERED MECHANICAL LOADS AND SKELETAL MUSCLE PHENOTYPE
    Richard Tsika; Fiscal Year: 2009
    ..abstract_text> ..
  50. MECHANISMS OF MYOGENIC INDUCTION AND PATTERN IN SOMITES
    Frank Stockdale; Fiscal Year: 1999
    ..Myogenesis is assessed by expression of fast and slow isoforms of myosin heavy chains and expression of myogenic regulatory factors...
  51. EFFECT OF CARDIOMYOPATHY MUTATIONS ON MYOSIN AND ACTIN
    KATHLEEN TRYBUS; Fiscal Year: 2004
    ....
  52. CONTRACTILE PROTEIN ACCUMULATION IN AIRWAY SMOOTH MUSCLE
    Julian Solway; Fiscal Year: 2002
    ..abstract_text> ..
  53. FHC Tn Mutations: Functional Consequences & Mechanisms
    James Potter; Fiscal Year: 2005
    ..T, troponin I and troponin C mutations under the same experimental conditions, and thus help identify key mechanism(s) involved in the pathogenesis of Troponin-linked FHC and lead to potential therapeutic strategies ..
  54. Structure & function of cardiac contractile proteins
    Jeffrey Robbins; Fiscal Year: 2004
    ..Together with the models above, it will provide a comprehensive picture of the alpha-MHC's effects on the development of hypertrophy, dilation and failure. ..
  55. FUNCTIONAL AND MOLECULAR STUDY OF HUMAN MASSETER FIBERS
    James Sciote; Fiscal Year: 2000
    ....
  56. CELL LINEAGE COMMITMENT IN MAMMALIAN MYOGENESIS
    Jeffrey Miller; Fiscal Year: 1991
    ..Methods will include clonal cell culture, neural tissue/somite co-culture, purification of cell types from neural tissue, immunocytochemistry, Western blotting, quantitative Northern blotting, and RNase protection assays...
  57. BIOCHEMICAL BASIS OF DEVELOPMENT IN DICTYOSTELIUM
    William Loomis; Fiscal Year: 1992
    ..By directly determining the essential cis-acting sequences in transformants we hope to recognize share cis-acting sequences that integrate expression of this set of genes...
  58. MOLECULAR MECHANISM FOR ERECTILE FUNCTION & DYSFUNCTION
    Michael Disanto; Fiscal Year: 2002
    ..It will also provide and explanation for changes in contractility of smooth muscle cells seen in patients with erectile dysfunction. ..
  59. Temporal and Spatial patterns of structural proteins in extraocular muscles
    CAROLE MONCMAN; Fiscal Year: 2009
    ..Information gained in this analysis may pave the road for the development of new therapeutic treatments for both eye muscle diseases and peripheral skeletal muscle diseases. ..
  60. GORDON RESEARCH CONFERENCE--MUSCLE CONTRACTILE PROTEINS
    PETER VIBERT; Fiscal Year: 1993
    ..The integrated approach to the study of muscle function proposed in this conference should yield a better understanding of the molecular mechanisms inherent to muscle contraction...
  61. CONTROL OF MYOSIN TYPES IN INDIVIDUAL MUSCLE FIBERS
    Neal Rubinstein; Fiscal Year: 1993
    The isoforms of vertebrate sarcomeric myosin heavy chains (MHC) are encoded by the members of a multigene family. Adult skeletal muscles contain a heterogeneous population of fibers which can be characterized by these MHC isoforms...
  62. ALPHA-1 ADRENERGIC REGULATION OF CARDIAC GENE EXPRESSION
    Paul Simpson; Fiscal Year: 1999
    ..phosphorylation of specific residues on TEF-1? Are these the same residues phosphorylated by a1-AR? Is PCK unique in this respect? Finally, do alteration of TEF-1 activity change transcription in myocyte culture and in the intact animal? ..
  63. DEVELOPMENTAL AGE AND CHANGES IN MYOSIN ISOZYMES
    Frank Stockdale; Fiscal Year: 1999
    ..and mammalian development, muscle fibers form in distinct generations and express disparate isoforms of myosin heavy chains (MyHCs)...
  64. Myosin Heavy Chain Polymorphism in Hybrid Muscle Fibers
    Scott Medler; Fiscal Year: 2009
    ..An understanding of muscle plasticity is central to our understanding of adaptation to exercise and to certain diseases such as cancer cachexia and age-related sarcopenia. ..