myosins

Summary

Summary: A diverse superfamily of proteins that function as translocating proteins. They share the common characteristics of being able to bind ACTINS and hydrolyse MgATP. Myosins generally consist of heavy chains which are involved in locomotion, and light chains which are involved in regulation. Within the structure of myosin heavy chain are three domains: the head, the neck and the tail. The head region of the heavy chain contains the actin binding domain and MgATPase domain which provides energy for locomotion. The neck region is involved in binding the light-chains. The tail region provides the anchoring point that maintains the position of the heavy chain. The superfamily of myosins is organized into structural classes based upon the type and arrangement of the subunits they contain.

Top Publications

  1. pmc Contraction and polymerization cooperate to assemble and close actomyosin rings around Xenopus oocyte wounds
    C A Mandato
    Department of Zoology, University of Wisconsin, Madison, WI 53706, USA
    J Cell Biol 154:785-97. 2001
  2. ncbi Recombinant motor domain constructs of Chara corallina myosin display fast motility and high ATPase activity
    Kohji Ito
    Department of Biology, Chiba University, Inage Ku, 263 8522, Chiba, Japan
    Biochem Biophys Res Commun 312:958-64. 2003
  3. pmc Myosin-dependent endoplasmic reticulum motility and F-actin organization in plant cells
    Haruko Ueda
    Department of Botany, Graduate School of Science, Kyoto University, Kyoto 606 8502, Japan
    Proc Natl Acad Sci U S A 107:6894-9. 2010
  4. pmc Drawing the tree of eukaryotic life based on the analysis of 2,269 manually annotated myosins from 328 species
    Florian Odronitz
    Department of NMR Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg, 37077 Goettingen, Germany
    Genome Biol 8:R196. 2007
  5. pmc Analysis of the actin-myosin II system in fish epidermal keratocytes: mechanism of cell body translocation
    T M Svitkina
    Laboratory of Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Cell Biol 139:397-415. 1997
  6. pmc Involvement of an actomyosin contractile ring in Saccharomyces cerevisiae cytokinesis
    E Bi
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    J Cell Biol 142:1301-12. 1998
  7. ncbi Myosins: a diverse superfamily
    J R Sellers
    National Heart, Lung and Blood Institute, National Institutes of Health, Building 10, Room 8N202, Bethesda, MD 20892, USA
    Biochim Biophys Acta 1496:3-22. 2000
  8. doi Diaphanous regulates myosin and adherens junctions to control cell contractility and protrusive behavior during morphogenesis
    Catarina C F Homem
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3280, USA
    Development 135:1005-18. 2008
  9. ncbi folded gastrulation, cell shape change and the control of myosin localization
    Rachel E Dawes-Hoang
    Department of Molecular Biology, Howard Hughes Medical Institute, Princeton University, NJ 08544, USA
    Development 132:4165-78. 2005
  10. pmc Targeted genomic capture and massively parallel sequencing to identify genes for hereditary hearing loss in Middle Eastern families
    Zippora Brownstein
    Department of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    Genome Biol 12:R89. 2011

Detail Information

Publications376 found, 100 shown here

  1. pmc Contraction and polymerization cooperate to assemble and close actomyosin rings around Xenopus oocyte wounds
    C A Mandato
    Department of Zoology, University of Wisconsin, Madison, WI 53706, USA
    J Cell Biol 154:785-97. 2001
    ....
  2. ncbi Recombinant motor domain constructs of Chara corallina myosin display fast motility and high ATPase activity
    Kohji Ito
    Department of Biology, Chiba University, Inage Ku, 263 8522, Chiba, Japan
    Biochem Biophys Res Commun 312:958-64. 2003
    ..Our results provide an excellent system to dissect the specific structural and functional features that distinguish the myosin responsible for fast cytoplasmic streaming...
  3. pmc Myosin-dependent endoplasmic reticulum motility and F-actin organization in plant cells
    Haruko Ueda
    Department of Botany, Graduate School of Science, Kyoto University, Kyoto 606 8502, Japan
    Proc Natl Acad Sci U S A 107:6894-9. 2010
    ..Although it was long assumed that the ER dynamics is actomyosin-driven, the responsible myosins were not identified, and the ER streaming was not characterized quantitatively...
  4. pmc Drawing the tree of eukaryotic life based on the analysis of 2,269 manually annotated myosins from 328 species
    Florian Odronitz
    Department of NMR Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg, 37077 Goettingen, Germany
    Genome Biol 8:R196. 2007
    ..Incongruence between phylogenetic trees can result from the violation of the orthology assumption and stochastic and systematic errors...
  5. pmc Analysis of the actin-myosin II system in fish epidermal keratocytes: mechanism of cell body translocation
    T M Svitkina
    Laboratory of Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Cell Biol 139:397-415. 1997
    ..We propose that the forward translocation of the cell body and retrograde flow in the lamellipodia are both driven by contraction of an actin-myosin network in the lamellipodial/cell body transition zone...
  6. pmc Involvement of an actomyosin contractile ring in Saccharomyces cerevisiae cytokinesis
    E Bi
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    J Cell Biol 142:1301-12. 1998
    ..cerevisiae. In its absence, cytokinesis can still be completed by a process (possibly localized cell-wall synthesis leading to septum formation) that appears to require septin function and to be facilitated by F-actin...
  7. ncbi Myosins: a diverse superfamily
    J R Sellers
    National Heart, Lung and Blood Institute, National Institutes of Health, Building 10, Room 8N202, Bethesda, MD 20892, USA
    Biochim Biophys Acta 1496:3-22. 2000
    b>Myosins constitute a large superfamily of actin-dependent molecular motors. Phylogenetic analysis currently places myosins into 15 classes. The conventional myosins which form filaments in muscle and non-muscle cells form class II...
  8. doi Diaphanous regulates myosin and adherens junctions to control cell contractility and protrusive behavior during morphogenesis
    Catarina C F Homem
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3280, USA
    Development 135:1005-18. 2008
    ..These effects also are mediated through coordinated effects on myosin activity and adhesion, suggesting a common mechanism for Diaphanous action during morphogenesis...
  9. ncbi folded gastrulation, cell shape change and the control of myosin localization
    Rachel E Dawes-Hoang
    Department of Molecular Biology, Howard Hughes Medical Institute, Princeton University, NJ 08544, USA
    Development 132:4165-78. 2005
    ..Our analysis of fog function therefore provides a direct link from patterning to cell shape change...
  10. pmc Targeted genomic capture and massively parallel sequencing to identify genes for hereditary hearing loss in Middle Eastern families
    Zippora Brownstein
    Department of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    Genome Biol 12:R89. 2011
    ..Our subjects for genome analysis are Israeli Jewish and Palestinian Arab families with hearing loss that varies in mode of inheritance and severity...
  11. ncbi Molecular basis of human Usher syndrome: deciphering the meshes of the Usher protein network provides insights into the pathomechanisms of the Usher disease
    Jan Reiners
    Institute of Zoology, Department of Cell and Matrix Biology, Johannes Gutenberg University of Mainz, Müllerweg 6, D 55099 Mainz, Germany
    Exp Eye Res 83:97-119. 2006
    ....
  12. pmc New insights into myosin evolution and classification
    Bernardo J Foth
    Department of Microbiology and Molecular Medicine, Centre Medical Universitaire, University of Geneva, 1 rue Michel Servet, 1211 Geneva, Switzerland
    Proc Natl Acad Sci U S A 103:3681-6. 2006
    b>Myosins are eukaryotic actin-dependent molecular motors important for a broad range of functions like muscle contraction, vision, hearing, cell motility, and host cell invasion of apicomplexan parasites...
  13. pmc The many different cellular functions of MYO7A in the retina
    David S Williams
    Department of Neurobiology and Ophthalmology, UCLA School of Medicine, Los Angeles, CA 90024, USA
    Biochem Soc Trans 39:1207-10. 2011
    ..It is likely that the progressive retinal degeneration that occurs in Usher syndrome 1B patients results from a combination of cellular defects in the RPE and photoreceptor cells...
  14. ncbi An actin-myosin complex on actively transcribing genes
    Nathalie Fomproix
    Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institute, Stockholm 171 77, Sweden
    Exp Cell Res 294:140-8. 2004
    ....
  15. ncbi Mechanism of force generation by myosin heads in skeletal muscle
    Gabriella Piazzesi
    Universita di Firenze, Viale G B Morgagni 63, I 50134 Firenze, Italy
    Nature 415:659-62. 2002
    ....
  16. pmc Myosin VIIa and sans localization at stereocilia upper tip-link density implicates these Usher syndrome proteins in mechanotransduction
    M Hamed Grati
    Laboratory of Cell Structure and Dynamics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 108:11476-81. 2011
    ..We propose that MYO7A, sans, and harmonin-b form the core components of the UTLD molecular complex. In this complex, MYO7A is likely the motor element that pulls on CDH23 to exert tension on the tip-link...
  17. pmc Specific nuclear localizing sequence directs two myosin isoforms to the cell nucleus in calmodulin-sensitive manner
    Rastislav Dzijak
    Department of Biology of the Cell Nucleus, Institute of Molecular Genetics of the ASCR, v v i, Prague, Czech Republic
    PLoS ONE 7:e30529. 2012
    ..NM1 differs from the "cytoplasmic" myosin 1c only by additional 16 amino acids at the N-terminus of the molecule. This amino acid stretch was therefore suggested to direct NM1 into the nucleus...
  18. ncbi ROCK and Dia have opposing effects on adherens junctions downstream of Rho
    Erik Sahai
    Cancer Research UK Centre for Cell and Molecular Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK
    Nat Cell Biol 4:408-15. 2002
    ..The ability of Dia1 to regulate the actin network is crucial for the localization of adherens junction components to the cell periphery...
  19. pmc Identification of the membrane receptor of a class XIV myosin in Toxoplasma gondii
    Elizabeth Gaskins
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, 108 Taylor Hall, CB 7090, Chapel Hill, NC 27599, USA
    J Cell Biol 165:383-93. 2004
    ..These results provide the first molecular description of an integral membrane protein as a specific receptor for a myosin motor, and further our understanding of the motile apparatus underlying gliding motility in apicomplexan parasites...
  20. pmc Cortical recruitment of nonmuscle myosin II in early syncytial Drosophila embryos: its role in nuclear axial expansion and its regulation by Cdc2 activity
    Anne Royou
    Centre National de la Recherche Scientifique, Centre de Genetique Moleculaire, 91198 Gif sur Yvette, France
    J Cell Biol 158:127-37. 2002
    ..These studies suggest a model in which cycles of myosin-mediated contraction and relaxation, tightly linked to Cdc2 and Rho kinase activity, are directly responsible for the axial expansion of the syncytial nuclei...
  21. pmc Abnormal phagocytosis by retinal pigmented epithelium that lacks myosin VIIa, the Usher syndrome 1B protein
    Daniel Gibbs
    Department of Pharmacology, School of Medicine, University of California at San Diego, La Jolla 92093 0912, USA
    Proc Natl Acad Sci U S A 100:6481-6. 2003
    ..Because the phagocytosis of photoreceptor disks by the RPE has been shown to be critical for photoreceptor cell viability, this defect likely contributes to the progressive blindness in USH1B...
  22. pmc Class XI myosins are required for development, cell expansion, and F-Actin organization in Arabidopsis
    Valera V Peremyslov
    Department of Botany and Plant Pathology and Center for Genome Research and Biocomputing, Oregon State University, Corvallis, Oregon 97331, USA
    Plant Cell 22:1883-97. 2010
    ..Although F-actin is essential for cell growth and plant development, roles of the associated myosins are poorly understood...
  23. pmc A comparative study of the involvement of 17 Arabidopsis myosin family members on the motility of Golgi and other organelles
    Dror Avisar
    Institute of Plant Sciences, Volcani Center, Bet Dagan 50250, Israel
    Plant Physiol 150:700-9. 2009
    ..Truncated fragments of all 17 annotated Arabidopsis myosins containing either the IQ tail or tail domains only were fused to fluorescent markers and coexpressed with a Golgi ..
  24. ncbi Phosphorylation and activation of myosin by Rho-associated kinase (Rho-kinase)
    M Amano
    Division of Signal Transduction, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma 630 01
    J Biol Chem 271:20246-9. 1996
    ..The activated Rho-kinase subsequently phosphorylates MLC. This may partly account for the mechanism by which Rho regulates cytokinesis, cell motility, or smooth muscle contraction...
  25. ncbi Mutant myosin VIIa causes defective melanosome distribution in the RPE of shaker-1 mice
    X Liu
    Nat Genet 19:117-8. 1998
  26. doi Unconventional myosins acting unconventionally
    Sarah Woolner
    Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester, UK
    Trends Cell Biol 19:245-52. 2009
    Unconventional myosins are proteins that bind actin filaments in an ATP-regulated manner...
  27. pmc Planar polarization of the atypical myosin Dachs orients cell divisions in Drosophila
    Yanlan Mao
    Apoptosis and Proliferation Control Laboratory, Cancer Research UK, London Research Institute, London WC2A 3PX, United Kingdom
    Genes Dev 25:131-6. 2011
    ..Planar polarization of Dachs is ultimately oriented by long-range gradients emanating from compartment boundaries, and is therefore a mechanism linking these gradients with the control of tissue shape...
  28. pmc Role of cortical tension in bleb growth
    Jean Yves Tinevez
    Max Planck Institute of Molecular Cell Biology and Genetics, 01 307 Dresden, Germany
    Proc Natl Acad Sci U S A 106:18581-6. 2009
    ..Together, our observations and model allow us to relate bleb shape parameters to the underlying cellular mechanics and provide insights as to how bleb formation can be biochemically regulated during cell motility...
  29. ncbi A Role of myosin Vb and Rab11-FIP2 in the aquaporin-2 shuttle
    Pavel I Nedvetsky
    Leibniz Institut fur Molekulare Pharmakologie FMP, Campus Berlin Buch, 13125 Berlin, Germany
    Traffic 8:110-23. 2007
    ..In summary, we identified myosin Vb as a motor protein involved in AQP2 recycling and show that myosin Vb- and Rab11-FIP2-dependent recycling of AQP2 is an integral part of the AQP2 shuttle...
  30. pmc Differing requirements for actin and myosin by plant viruses for sustained intercellular movement
    Phillip A Harries
    Plant Biology Division, The Samuel Roberts Noble Foundation, Inc 2510 Sam Noble Parkway, Ardmore, OK 73401, USA
    Proc Natl Acad Sci U S A 106:17594-9. 2009
    ..Here we determine the importance of microfilaments and myosins for the sustained intercellular movement of a group of RNA-based plant viruses...
  31. ncbi The ubiquitin-selective chaperone CDC-48/p97 links myosin assembly to human myopathy
    Philipp Christoph Janiesch
    Centre for Molecular Neurobiology ZMNH, University of Hamburg, Falkenried 94, 20251 Hamburg, Germany
    Nat Cell Biol 9:379-90. 2007
    ....
  32. pmc RhoA is required for monocyte tail retraction during transendothelial migration
    R A Worthylake
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Cell Biol 154:147-60. 2001
    ..Finally, we find that p160ROCK signaling negatively regulates integrin adhesions and that inhibition of RhoA results in an accumulation of beta2 integrin in the unretracted tails...
  33. ncbi Chaperone-mediated folding and assembly of myosin in striated muscle
    Rajani Srikakulam
    Department of Pathology and Laboratory Medicine, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, NJ 08854, USA
    J Cell Sci 117:641-52. 2004
    ..We conclude that multimeric complexes of nascent myosin filaments associated with Hsc70 and Hsp90 are intermediates in the folding and assembly pathway of muscle myosin...
  34. ncbi Human myosin-Vc is a novel class V myosin expressed in epithelial cells
    Olga C Rodriguez
    Department of Cell and Molecular Physiology, Medical Science Research Building, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Cell Sci 115:991-1004. 2002
    Class V myosins are one of the most ancient and widely distributed groups of the myosin superfamily and are hypothesized to function as motors for actin-dependent organelle transport...
  35. pmc The unconventional myosin-VIIa associates with lysosomes
    Lily E Soni
    Section of Cell and Developmental Biology, Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093 0368, USA
    Cell Motil Cytoskeleton 62:13-26. 2005
    ..Myosin-VIIa copurified with lysosomes on density gradients, and fractionation and extraction experiments suggested that it was tightly associated with the lysosome surface. These studies suggest that myosin-VIIa is a lysosome motor...
  36. doi Utilization of myosin and actin bundles for the transport of molecular cargo
    Hideyo Takatsuki
    Laboratory of Molecular Physiology, Robert C Byrd Biotechnology Science Center, Department of Biological Sciences, Marshall University, Huntington, WV 25755 1090, USA
    Small 6:452-7. 2010
    ..These data suggest that actin bundling may significantly improve the applicability of the myosin motor for future nanotechnological applications...
  37. pmc Functional characterization of the human myosin-7a motor domain
    Sarah M Heissler
    Institut für Biophysikalische Chemie, Medizinische Hochschule Hannover, Hannover, Germany
    Cell Mol Life Sci 69:299-311. 2012
    ..Our results suggest that in a cellular environment, compartment-specific fluctuations in free Mg(2+) ions can mediate the conditional switching of myosin-7a between cargo moving and tension bearing modes...
  38. doi A myosin XI tail domain homologous to the yeast myosin vacuole-binding domain interacts with plastids and stromules in Nicotiana benthamiana
    Amir Sattarzadeh
    Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
    Mol Plant 2:1351-8. 2009
    ..The involvement of myosins in chloroplast positioning and mitochondrial movement was demonstrated by expression of a virus-induced gene ..
  39. pmc Build it up-Tear it down: protein quality control in the cardiac sarcomere
    Monte S Willis
    Carolina Cardiovascular Biology Center, University of North Carolina, 8200 Medical Biomolecular Research Bldg, 103 Mason Farm Road, Chapel Hill, NC 27599 7126, USA
    Cardiovasc Res 81:439-48. 2009
    ..In this review, we highlight the dynamic interplay between sarcomere-specific chaperones and ubiquitin-dependent degradation of sarcomere proteins that is necessary in order to maintain structure and function of the cardiac sarcomere...
  40. doi Nuclear actin and myosins: life without filaments
    Primal de Lanerolle
    Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, Illinois 60612, USA
    Nat Cell Biol 13:1282-8. 2011
    ..were traditionally thought to function only in the cytoplasm, it is now well accepted that actin and multiple myosins are found in the nucleus...
  41. ncbi Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1
    K Ebnet
    Institute of Cell Biology, ZMBE, University of Muenster, D 48149 Muenster, Germany
    J Biol Chem 275:27979-88. 2000
    ..Our results suggest that JAM can be recruited to intercellular junctions by its interaction with the PDZ domain-containing proteins AF-6 and possibly ZO-1...
  42. pmc Myosin vb is associated with plasma membrane recycling systems
    L A Lapierre
    Departments of Medicine, Surgery, and Cellular Biology and Anatomy, Institute of Molecular Medicine and Genetics, Medical College of Georgia and the Augusta VA Medical Center, Augusta, Georgia 30912, USA
    Mol Biol Cell 12:1843-57. 2001
    ..The dominant negative effects of the myosin Vb tail chimera indicate that this unconventional myosin is required for transit out of plasma membrane recycling systems...
  43. pmc Sequential assembly of myosin II, an IQGAP-like protein, and filamentous actin to a ring structure involved in budding yeast cytokinesis
    J Lippincott
    Department of Cell Biology, Harvard Medical School, Boston, Massachussetts 02115, USA
    J Cell Biol 140:355-66. 1998
    ....
  44. pmc Tobacco mosaic virus infection spreads cell to cell as intact replication complexes
    Shigeki Kawakami
    Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, MO 63132, USA
    Proc Natl Acad Sci U S A 101:6291-6. 2004
    ..We propose a model whereby cell-cell spread of tobamovirus infection is accomplished by subviral replication complexes that initiate TMV replication immediately after entry to adjacent cells...
  45. ncbi Update on Usher syndrome
    Zubin Saihan
    UCL Institute of Ophthalmology and Moorfields Eye Hospital, London, UK
    Curr Opin Neurol 22:19-27. 2009
    ..The present review addresses the mechanisms, genetics and pathogenesis of Usher syndrome...
  46. pmc Expression in cochlea and retina of myosin VIIa, the gene product defective in Usher syndrome type 1B
    T Hasson
    Department of Biology, Yale University, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 92:9815-9. 1995
    ....
  47. ncbi Specificity of blebbistatin, an inhibitor of myosin II
    John Limouze
    Laboratory of Molecular Cardiology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Muscle Res Cell Motil 25:337-41. 2004
    ..Blebbistatin potently inhibits several striated muscle myosins as well as vertebrate nonmuscle myosin IIA and IIB with IC50 values ranging from 0.5 to 5 microM...
  48. pmc Afadin: A novel actin filament-binding protein with one PDZ domain localized at cadherin-based cell-to-cell adherens junction
    K Mandai
    Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c o JCR Pharmaceuticals Co, Ltd, Kobe 651 22, Japan
    J Cell Biol 139:517-28. 1997
    ..These results suggest that l-afadin serves as a linker of the actin cytoskeleton to the plasma membrane at cell-to-cell AJ...
  49. pmc A high precision survey of the molecular dynamics of mammalian clathrin-mediated endocytosis
    Marcus J Taylor
    Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom
    PLoS Biol 9:e1000604. 2011
    ....
  50. ncbi Functional analysis of the Drosophila diaphanous FH protein in early embryonic development
    K Afshar
    Department of Biology, Center for Molecular Genetics, University of California, San Diego, La Jolla CA 92093 0634, USA
    Development 127:1887-97. 2000
    ..Based on known biochemical functions of FH proteins, we propose that Diaphanous serves as a mediator between signaling molecules and actin organizers at specific phases of the cell cycle...
  51. pmc Phosphorylation switches specific for the cardiac isoform of myosin binding protein-C: a modulator of cardiac contraction?
    M Gautel
    European Molecular Biology Laboratory, Heidelberg, Germany
    EMBO J 14:1952-60. 1995
    ..2 in humans, and is therefore in a region of physical linkage to subsets of familial hypertrophic cardiomyopathy (FHC). This makes cardiac MyBP-C a candidate gene for chromosome 11-associated FHC...
  52. pmc Class VIII myosins are required for plasmodesmatal localization of a closterovirus Hsp70 homolog
    Dror Avisar
    Department of Botany and Plant Pathology, Oregon State University, Cordley Hall 2082, Corvallis, OR 97331, USA
    J Virol 82:2836-43. 2008
    ..I. Prokhnevsky, V. V. Peremyslov, and V. V. Dolja, J. Virol. 79:14421-14428, 2005). Myosins are a diverse category of molecular motors that possess a motor domain and a tail domain involved in cargo ..
  53. pmc Myosin XI is essential for tip growth in Physcomitrella patens
    Luis Vidali
    Department of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, Massachusetts 01609, USA
    Plant Cell 22:1868-82. 2010
    Class XI myosins are plant specific and responsible for cytoplasmic streaming. Because of the large number of myosin XI genes in angiosperms, it has been difficult to determine their precise role, particularly with respect to tip growth...
  54. pmc Dynamics and regulation of contractile actin-myosin networks in morphogenesis
    Karen E Kasza
    Howard Hughes Medical Institute, Developmental Biology Program, Sloan Kettering Institute, 1275 York Avenue, New York, NY 10065, USA
    Curr Opin Cell Biol 23:30-8. 2011
    ..Analysis of the complex behaviors of actin-myosin networks will be crucial for understanding force generation in actively remodeling cells and the coordination of cell shape and movement at the tissue level...
  55. pmc Expression, splicing, and evolution of the myosin gene family in plants
    Valera V Peremyslov
    Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331, USA
    Plant Physiol 155:1191-204. 2011
    Plants possess two myosin classes, VIII and XI. The myosins XI are implicated in organelle transport, filamentous actin organization, and cell and plant growth...
  56. pmc In the thick of it: HCM-causing mutations in myosin binding proteins of the thick filament
    Samantha P Harris
    Department of Neurobiology, Physiology, and Behavior College of Biological Sciences, University of California, One Shields Ave, Davis, CA 95616, USA
    Circ Res 108:751-64. 2011
    ..Additional work is still needed to determine the mechanisms by which individual mutations induce hypertrophic phenotypes...
  57. ncbi Reduced climbing and increased slipping adaptation in cochlear hair cells of mice with Myo7a mutations
    C J Kros
    School of Biological Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK
    Nat Neurosci 5:41-7. 2002
    ..We conclude that myosin VIIA participates in anchoring and holding membrane-bound elements to the actin core of the stereocilium. Myosin VIIA is therefore required for the normal gating of transducer channels...
  58. pmc Myosin XVa localizes to the tips of inner ear sensory cell stereocilia and is essential for staircase formation of the hair bundle
    Inna A Belyantseva
    Laboratory of Molecular Genetics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, 5 Research Court, Rockville, MD 20850, USA
    Proc Natl Acad Sci U S A 100:13958-63. 2003
    ..We propose that myosin XVa is essential for the graded elongation of stereocilia during their functional maturation...
  59. ncbi Shaker-1 mutations reveal roles for myosin VIIA in both development and function of cochlear hair cells
    T Self
    MRC Institute of Hearing Research, University Park, Nottingham NG7 2RD, UK
    Development 125:557-66. 1998
    ..These findings suggest that myosin VIIA is required for normal stereocilia bundle organisation and has a role in the function of cochlear hair cells...
  60. pmc Myosin-10 and actin filaments are essential for mitotic spindle function
    Sarah Woolner
    Department of Zoology, University of Wisconsin Madison, Madison, WI 53706, USA
    J Cell Biol 182:77-88. 2008
    ..in mitotic spindles are well established, whether or not actin filaments (F-actin) and F-actin-based motors (myosins) are required components of mitotic spindles has long been controversial...
  61. pmc Movement and remodeling of the endoplasmic reticulum in nondividing cells of tobacco leaves
    I Sparkes
    School of Life Sciences, Oxford Brookes University, Oxford OX3 0BP, United Kingdom
    Plant Cell 21:3937-49. 2009
    ..Expression of a subset of truncated class XI myosins, which interfere with the activity of native class XI myosins, and drug-induced actin depolymerization produce a ..
  62. pmc Myosin Vs organize actin cables in fission yeast
    Libera Lo Presti
    Department of Fundamental Microbiology, University of Lausanne, CH 1015 Lausanne, Switzerland
    Mol Biol Cell 23:4579-91. 2012
    ..Together these in vivo data reveal elements of a self-organizing system in which the motors shape their own tracks by transporting cargoes and exerting physical pulling forces...
  63. ncbi Wound-induced assembly and closure of an actomyosin purse string in Xenopus oocytes
    W M Bement
    Department of Zoology, Program in Cellular and Molecular Biology, University of Wisconsin Madison, 1117 West Johnson Street, Madison, Wisconsin 53706, USA
    Curr Biol 9:579-87. 1999
    ....
  64. pmc Functional roles for myosin 1c in cellular signaling pathways
    Lisa M Bond
    Cambridge Institute for Medical Research, University of Cambridge, Wellcome Trust MRC Building, Hills Road, Cambridge, CB2 0XY, United Kingdom
    Cell Signal 25:229-35. 2013
    ..This review provides an overview of the functional involvement of myosin 1c in cellular signaling and discusses the possible potential for myosin 1c as a target for drug-based treatments for human diseases...
  65. ncbi Inhibition of myosin light chain kinase by p21-activated kinase
    L C Sanders
    Departments of Immunology and Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Science 283:2083-5. 1999
    ..These data indicate that MLCK is a target for PAKs and that PAKs may regulate cytoskeletal dynamics by decreasing MLCK activity and MLC phosphorylation...
  66. pmc Kinetic and equilibrium analysis of the myosin ATPase
    Enrique M De La Cruz
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, USA
    Methods Enzymol 455:157-92. 2009
    ..ATPase pathway vary significantly across the myosin superfamily, resulting in kinetic differences that that allow myosins to carry out diverse mechanical functions...
  67. pmc Missense mutations in the beta-myosin heavy-chain gene cause central core disease in hypertrophic cardiomyopathy
    L Fananapazir
    Cardiology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 90:3993-7. 1993
    ..This work demonstrates that (i) MYH7-associated HCM is often a disease of striated muscle but with predominant cardiac involvement and (ii) a subset of HCM patients with MYH7 gene missense mutations have CCD...
  68. ncbi Chromatin remodelling and transcription: be-WICHed by nuclear myosin 1
    Piergiorgio Percipalle
    Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institute, P O Box 285, Stockholm SE 171 77, Sweden
    Curr Opin Cell Biol 18:267-74. 2006
    ..NoRC produces a repressive chromatin state; B-WICH, together with nuclear myosin 1, activates pol I transcription directly on chromatin templates and might also function in the maintenance of ribosomal chromatin structure...
  69. ncbi Rigor-like structures from muscle myosins reveal key mechanical elements in the transduction pathways of this allosteric motor
    Yuting Yang
    Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454, USA
    Structure 15:553-64. 2007
    ..The overall arrangements of subdomains in the motor are relatively conserved in each of the known contractile states, and we explore qualitatively the energetics of these states...
  70. pmc Myosin-X: a MyTH-FERM myosin at the tips of filopodia
    Michael L Kerber
    Department of Cell and Molecular Physiology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7545, USA
    J Cell Sci 124:3733-41. 2011
    ..At the cellular and organismal level, growing evidence demonstrates that Myo10 has crucial functions in numerous processes ranging from invadopodia formation to cell migration...
  71. pmc Requirement of myosin Vb.Rab11a.Rab11-FIP2 complex in cholesterol-regulated translocation of NPC1L1 to the cell surface
    Bei Bei Chu
    State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China
    J Biol Chem 284:22481-90. 2009
    ..These results suggest that the efficient transport of NPC1L1 to the PM is dependent on the microfilament-associated myosin Vb.Rab11a.Rab11-FIP2 triple complex...
  72. pmc A mechanical model of actin stress fiber formation and substrate elasticity sensing in adherent cells
    Sam Walcott
    Mechanical Engineering and Johns Hopkins Physical Science Oncology Center, Johns Hopkins University, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 107:7757-62. 2010
    ..As these large bundles of actin, called stress fibers, appear preferentially on stiff surfaces, our mechanical model provides a mechanism for stress fiber formation and stiffness sensing in cells adhered to a compliant surface...
  73. pmc Role of phosphatidylinositol 4-phosphate (PI4P) and its binding protein GOLPH3 in hepatitis C virus secretion
    Bryan Bishe
    Division of Infectious Diseases, Moores Cancer Center, University of California, San Diego, La Jolla, California 92093, USA
    J Biol Chem 287:27637-47. 2012
    ..These studies establish the role of PI4P and its interacting protein GOLPH3 in HCV secretion and strengthen the significance of the Golgi secretory pathway in this process...
  74. ncbi Localization of the rat myosin I molecules myr 1 and myr 2 and in vivo targeting of their tail domains
    C Ruppert
    Friedrich Miescher Laboratorium der Max Planck Gesellschaft, Tubingen, Germany
    J Cell Sci 108:3775-86. 1995
    ..These results suggest that the tail domains of myr 1 and myr 2 are sufficient for subcellular targeting but that their head domains also contribute significantly to maintaining a proper subcellular localization...
  75. pmc Coupling actin flow, adhesion, and morphology in a computational cell motility model
    Danying Shao
    Center for Theoretical Biological Physics and Department of Physics, University of California, San Diego, La Jolla, CA 92093 0374, USA
    Proc Natl Acad Sci U S A 109:6851-6. 2012
    ..Our model suggests that the pattern of the actin flow inside the cell, the cell velocity, and the cell morphology are determined by the integration of actin polymerization, myosin contraction, adhesion forces, and membrane forces...
  76. pmc Comparative kinetic and functional characterization of the motor domains of human nonmuscle myosin-2C isoforms
    Sarah M Heissler
    Institute for Biophysical Chemistry, Hannover Medical School, 30625 Hannover, Germany
    J Biol Chem 286:21191-202. 2011
    Nonmuscle myosins are widely distributed and play important roles in the maintenance of cell morphology and cytokinesis...
  77. doi A novel actin binding site of myosin required for effective muscle contraction
    Boglárka H Várkuti
    Department of Biochemistry, Eotvos Lorand University, Budapest, Hungary
    Nat Struct Mol Biol 19:299-306. 2012
    ..We conclude that the binding of actin to myosin's activation loop specifically increases the ratio of mechanically productive to futile myosin heads, leading to efficient muscle contraction...
  78. pmc Control of actin moving trajectory by patterned poly(methylmethacrylate) tracks
    H Suzuki
    Kansai Advanced Research Center, Communications Research Laboratory, Kobe, Japan
    Biophys J 72:1997-2001. 1997
    ..The mean velocity of actin movement on the PMMA was 4.5 mm/s at 25 degrees C, and it was comparable to that on a conventionally used nitrocellulose film...
  79. pmc Domain structure of a mammalian myosin I beta
    O Reizes
    Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas 75235
    Proc Natl Acad Sci U S A 91:6349-53. 1994
    ..5-kDa domain that contains the ATP- and actin-binding sites, followed by an 8.5-kDa domain with three calmodulin-binding sequences and a basic 30-kDa carboxyl-terminal tail segment that binds to anionic phospholipids and membranes...
  80. ncbi Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA
    R M Long
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Science 277:383-7. 1997
    ..A cis-acting element in the 3'-untranslated region of ASH1 mRNA is sufficient to localize a chimeric RNA to daughter cells. These results suggest that localization of mRNA may have been an early property of the eukaryotic lineage...
  81. pmc Cooperative [Ca²+]-dependent regulation of the rate of myosin binding to actin: solution data and the tropomyosin chain model
    Michael Geeves
    Department of Biosciences, University of Kent, Canterbury, Kent, United Kingdom
    Biophys J 100:2679-87. 2011
    ..Implications for theories of thin-filament regulation in muscle are discussed...
  82. doi Deficiency of Myo18B in mice results in embryonic lethality with cardiac myofibrillar aberrations
    Rieko Ajima
    Biology Division, National Cancer Center Research Institute, Tokyo 104 0045, Japan
    Genes Cells 13:987-99. 2008
    Myo18B is an unconventional myosin family protein expressed predominantly in muscle cells. Although conventional myosins are known to be localized on the A-bands and function as a molecular motor for muscle contraction, Myo18B protein ..
  83. pmc A FERM domain autoregulates Drosophila myosin 7a activity
    Yi Yang
    Laboratory of Molecular Physiology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 106:4189-94. 2009
    ..We propose that at low cellular actin concentrations, myosin 7a-FL is bent and inactive, but at high actin concentrations, it is unfolded and active because the C-terminal FERM domain binds to actin...
  84. ncbi Mother cell-specific HO expression in budding yeast depends on the unconventional myosin myo4p and other cytoplasmic proteins
    R P Jansen
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 84:687-97. 1996
    ..In an accompanying paper, we show that SHE genes are needed for the accumulation in daughter nuclei of Ash1p, a repressor of HO...
  85. pmc Myosin IXa regulates epithelial differentiation and its deficiency results in hydrocephalus
    Marouan Abouhamed
    Institute of General Zoology and Genetics, Westfalian Wilhelms University, 48149 Munster, Germany
    Mol Biol Cell 20:5074-85. 2009
    ..Moreover, Rho-kinases may represent a new target for therapeutic intervention in some forms of hydrocephalus...
  86. pmc The effect of ageing and immobilization on structure and function of human skeletal muscle fibres
    Giuseppe D'Antona
    Department of Experimental Medicine, Human Physiology Unit, University of Pavia, Pavia, Italy
    J Physiol 552:499-511. 2003
    ....
  87. ncbi Characterization of myosin-IA and myosin-IB, two unconventional myosins associated with the Drosophila brush border cytoskeleton
    N S Morgan
    Department of Genetics, School of Medicine, Yale University, New Haven, Connecticut 06520, USA
    Dev Biol 172:51-71. 1995
    The expression patterns of myosin-IA (MIA) and myosin-IB (MIB), two novel unconventional myosins from Drosophila melanogaster, have been characterized through immunoblot analysis and immunocytochemistry of embryos, larvae, and adults...
  88. pmc A Rictor-Myo1c complex participates in dynamic cortical actin events in 3T3-L1 adipocytes
    G Nana Hagan
    Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
    Mol Cell Biol 28:4215-26. 2008
    ..Taken together, our findings suggest that the Rictor-Myo1c complex is distinct from mTORC2 and that Myo1c, in conjunction with Rictor, participates in cortical actin remodeling events...
  89. pmc Mechanism and specificity of pentachloropseudilin-mediated inhibition of myosin motor activity
    Krishna Chinthalapudi
    Research Centre for Structural Analysis, Hannover Medical School, Hannover, Germany
    J Biol Chem 286:29700-8. 2011
    ..IC(50) values are in the range from 1 to 5 μm for mammalian class-1 myosins and greater than 90 μm for class-2 and class-5 myosins, and no inhibition was observed with class-6 and class-7 ..
  90. pmc Myosin XI-K Is required for rapid trafficking of Golgi stacks, peroxisomes, and mitochondria in leaf cells of Nicotiana benthamiana
    Dror Avisar
    Department of Botany and Plant Pathology and Center for Genome Research and Biocomputing, Oregon State University, Corvallis, OR 97331, USA
    Plant Physiol 146:1098-108. 2008
    ..We sequenced six complete Nicotiana benthamiana cDNAs that encode class XI and class VIII myosins. Phylogenetic analysis indicates that these two classes of myosins diverged prior to the radiation of green algae ..
  91. pmc Effects of a R133W beta-tropomyosin mutation on regulation of muscle contraction in single human muscle fibres
    Julien Ochala
    Department of Clinical Neurophysiology, Uppsala University Hospital, Entrance 85, 3rd Floor, SE 751 85 Uppsala, Sweden
    J Physiol 581:1283-92. 2007
    ....
  92. pmc Antibodies covalently immobilized on actin filaments for fast myosin driven analyte transport
    Saroj Kumar
    School of Natural Sciences, Linnaeus University, Kalmar, Sweden
    PLoS ONE 7:e46298. 2012
    ....
  93. pmc Mature mice lacking Rbl2/p130 gene have supernumerary inner ear hair cells and supporting cells
    Sonia M Rocha-Sanchez
    Department of Oral Biology, Creighton University School of Dentistry, Omaha, Nebraska 68178, USA
    J Neurosci 31:8883-93. 2011
    ..Interestingly, p130(-/-) mice exhibited nearly normal peripheral auditory sensitivity...
  94. pmc Myosin Vc is a molecular motor that functions in secretory granule trafficking
    Damon T Jacobs
    Department of Cell and Molecular Physiology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Biol Cell 20:4471-88. 2009
    Class V myosins are actin-based motor proteins that have critical functions in organelle trafficking...
  95. pmc Myosin motor Myo1c and its receptor NEMO/IKK-gamma promote TNF-alpha-induced serine307 phosphorylation of IRS-1
    Yoshitaka Nakamori
    Division of Molecular Analysis of Human Disorders, Department of Bio Signal Analysis, Yamaguchi University Graduate School of Medicine, Ube 755 8505, Japan
    J Cell Biol 173:665-71. 2006
    ..Thus, motor protein Myo1c and its receptor protein NEMO act cooperatively to form the IKK-IRS-1 complex and function in TNF-alpha-induced insulin resistance...
  96. pmc Dynamic regulation of sarcomeric actin filaments in striated muscle
    Shoichiro Ono
    Department of Pathology and Department of Cell Biology, Emory University, Atlanta, Georgia 30322, USA
    Cytoskeleton (Hoboken) 67:677-92. 2010
    ..Further investigation of the regulatory mechanism of actin dynamics in striated muscle should be a key to understanding how myofibrils develop and operate...
  97. pmc Identification of two type V myosins in fission yeast, one of which functions in polarized cell growth and moves rapidly in the cell
    F Motegi
    Division of Biology, Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Tokyo, 153-8902, Japan
    Mol Biol Cell 12:1367-80. 2001
    ..In contrast, the phenotype of myo5 null cells was indistinguishable from that of wild-type cells. This and other data suggest that Myo5 has a role distinct from that of Myo4...
  98. ncbi Involvement of actin and myosins in Plasmodium berghei ookinete motility
    Inga Siden-Kiamos
    Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Vassilika Vouton, P O Box 1385, 71110 Heraklion, Crete, Greece
    Mol Biochem Parasitol 150:308-17. 2006
    ..We also studied the expression during ookinete development of genes encoding actin and two small class XIV myosins, PbMyoA, and PbMyoB...
  99. pmc Drosophila UNC-45 accumulates in embryonic blastoderm and in muscles, and is essential for muscle myosin stability
    Chi F Lee
    Department of Biology and the Molecular Biology Institute, San Diego State University, San Diego, CA 92182, USA
    J Cell Sci 124:699-705. 2011
    ..Overall, our data indicate that dUNC-45 is important for myosin accumulation and muscle function. Furthermore, our results suggest that dUNC-45 acts post-translationally for proper myosin folding and maturation...
  100. pmc Human Myo19 is a novel myosin that associates with mitochondria
    Omar A Quintero
    Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Curr Biol 19:2008-13. 2009
    ..Defects in their localization and dynamics lead to human disease. Myosins are actin-based motors that power processes such as muscle contraction, cytokinesis, and organelle transport...
  101. ncbi Novel mutations of MYO15A associated with profound deafness in consanguineous families and moderately severe hearing loss in a patient with Smith-Magenis syndrome
    N Liburd
    Laboratory of Molecular Genetics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, 5 Research Court, Room 2A 015, Rockville, MD 20850, USA
    Hum Genet 109:535-41. 2001
    ..In addition, one hemizygous missense mutation of MYO15A was found in one of eight Smith-Magenis syndrome (del(17)p11.2) patients from North America who had moderately severe sensorineural hearing loss...

Research Grants115 found, 100 shown here

  1. Chymase-Mediated MMP Activation in Ishemia Reperfusion Injury
    LOUIS JOSEPH DELL'ITALIA; Fiscal Year: 2013
    ....
  2. Neurotrophins in the Lung
    Y S Prakash; Fiscal Year: 2012
    ..By establishing the role of neurotrophins in airway narrowing with or without inflammation, the proposed studies will the foundation for better understanding of airway diseases, and potential development of new therapeutic targets. ..
  3. Dynamics of Kinesins, Dyneins, and Myosins
    Paul R Selvin; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Molecular motors - kinesin, dynein, and myosins - play a crucial role in the maintenances and development of the organization, motility, and cell signaling, of healthy cells...
  4. Cellular and Molecular Regulation of Thrombopoiesis
    Ramesh A Shivdasani; Fiscal Year: 2012
    ..Characterizing key molecular pathways of platelet formation should lead to better control of circulating platelet counts and to implementation of less toxic treatments for cancer. ..
  5. Myosin phosphatase autoinhibition in gastrointestinal smooth muscle contraction
    Avril V Somlyo; Fiscal Year: 2012
    ..The results of the research should translate into novel treatments for targeting these diseases. ..
  6. EFFECT OF LIPIDS ON VASCULAR GRAFT HEALING
    Linda M Graham; Fiscal Year: 2013
    ..For example, a TRPC6 inhibitor could increase endothelial cell migration and promote healing of arterial injuries and vascular grafts for the benefit all patients who require cardiovascular interventions. ..
  7. Zot, Zonulin, and Pathophysiology of Intestinal Tight Junctions
    Alessio Fasano; Fiscal Year: 2013
    ..With our Aim 3 we will investigate the association of HP genotypes (HP1-1, HP1-2, and HP2-2) with serum zonulin expression and clinical severity in human disease states and transgenic murine models. ..
  8. Impact of AMP-activated kinase on sex differences in hypertrophic cardiomyopathy
    John P Konhilas; Fiscal Year: 2013
    ..Therefore, it is of major clinical significance that the mechanistic link be determined between cardiac disease, sex/gender differences and energetic regulators in the heart. ) ..
  9. Molecular control of differential peptide transmitter exocytosis.
    COREY B SMITH; Fiscal Year: 2013
    ..The data obtained will provide a molecular understanding of the key regulators of the acute sympatho-adrenal stress response as well as pathologies resulting from its improper regulation. ..
  10. Autoimmunity in the Mediation of Infectious Myocarditis
    Jay Reddy; Fiscal Year: 2013
    ..These studies may then create opportunities to derive immunotherapies in the form of altered peptide ligands and tolerogens as antigen-specific treatment modalities, including personalized therapies, for DCM patients.] ..
  11. Hypoplastic Left Heart Syndrome: Expression of RHD in the Fetus?
    Pirooz Eghtesady; Fiscal Year: 2013
    ..Tremendous resources are devoted to its medical management. Therefore, understanding why HLHS happens, the goal of the proposed research would have great significance and relevance to public health. ..
  12. Human Platelet Defects in Transcription Factor RUNX1 Haplodeficiency
    ANGARA KONETI RAO; Fiscal Year: 2013
    ..They will advance our understanding of the mechanisms in normal and abnormal platelet function. ..
  13. SMOOTH MUSCLE MYOSIN PHOSPHATASE SUBUNIT ISOFORMS
    Steven A Fisher; Fiscal Year: 2013
    ..These studies will provide novel insights regarding the role of Tra2b, and novel links between transcriptional and splicing controls, in the generation and modulation of vascular smooth muscle contractile phenotypic diversity. ..
  14. The Effect Of Deafness Associated Mutations on MyosinVIIA Function
    Eva Forgacs; Fiscal Year: 2010
    ..The biochemical, structural and motile properties of the wild type and mutant myosin VIIA proteins will be studied, to delineate the molecular mechanism of the myosin VIIA related deafness, vestibular dysfunction and blindness. ..
  15. NFATc3 in chronic hypoxic pulmonary hypertension
    Laura V Gonzalez Bosc; Fiscal Year: 2012
    ..A better understanding of this mechanisms in PAH will lead to the development of novel therapeutic approaches to prevent and cure this debilitating disease. ..
  16. Silencing cardiac-specific microRNAs to prevent pathological remodeling in conges
    MATTHEW SWAN LAWRENCE; Fiscal Year: 2011
    ..Where rodent hearts express more 1MHC, monkey hearts, like human hearts, predominantly express 2M HC. Both myosins co-express a miRNA-208 isoform that we will designate as miR- 208a (coming from 1MHC) and miR-208b (coming from ..
  17. Cardiomyocyte Zinc and its Regulation of Contraction-Relaxation Function
    Bradley M Palmer; Fiscal Year: 2012
    ..This study could therefore provide a basis for therapies for several diseases or conditions that affect a large component of the population. ..
  18. Mechanisms of Arsenic-Induced Developmental Toxicity
    Lisa Bain; Fiscal Year: 2009
    ....
  19. Endothelial Lipoxygenase Metabolites and Vascular Tone
    WILLIAM BRYSON CAMPBELL; Fiscal Year: 2013
    ..These studies will identify two new mechanisms of action for the endothelium-derived relaxing factor 11,12,15-THETA and provide a new therapeutic approach to enhancing endothelial function. ..
  20. Mechanics and regulation of chromosome dynamics in meiotic prophase
    MICHAEL EDWARD DRESSER; Fiscal Year: 2012
    ..results establish a role for the myosin type II gene MYO1 in a subset of the RPMs and suggest that other myosins influence RPMs either directly or by affecting the rate of actin filament treadmilling...
  21. Regulation of Endothelial ICAM-1 and PMN-Mediated Lung Injury by Actin Dynamics
    Fabeha Fazal; Fiscal Year: 2013
    ....
  22. Myosin 15:Genetics, Pathology and Therapeutic Potential
    Sally A Camper; Fiscal Year: 2013
    ..This team will enable us to exploit the animal models fully to understand the mechanisms of inner ear disease and has the potential to identify novel genes essential for normal hearing. ..
  23. Regulation of Intestinal Epithelial Tight Junction Barrier
    Thomas Y Ma; Fiscal Year: 2011
    ..The purpose of this grant application is to elucidate the pathogenic mechanisms involved in bacterial antigen induced disruption of intestinal barrier (or development of leaky gut). ..
  24. Understanding the biology of schistosomes in response to praziquantel
    Charles Cunningham; Fiscal Year: 2013
    ..More explicit knowledge of the binding target of PZQ and the mechanism of action of the drug will help us to devise improved assays for monitoring the emergence of resistance. ..
  25. Development of an Inhibitor of Toxoplasma gondii
    Richard D Wood; Fiscal Year: 2010
    ..gondii, as treatments for toxoplasmosis which affects HIV/AIDS patients. ..
  26. Keratin Biology and Disease Aspects in Simple Epithelia
    Bishr Omary; Fiscal Year: 2013
    ..Our ultimate goal is to better understand the function of keratins and their human disease relevance in digestive organs, and to provide fundamental knowledge on how the keratins family regulates normal digestive organ physiology. ..
  27. Cellular Mechanisms of Lymphatic Muscle Contractility
    MICHAEL JOHN DAVIS; Fiscal Year: 2012
    ..These studies will investigate which proteins allow these vessels to contract so that specific therapeutic agents can be developed to correct lymphatic pump dysfunction and drainage of edematous tissues. ..
  28. EFFECT OF R1R2 OVER-EXPRESSION ON CARDIAC FUNCTION
    Michael Regnier; Fiscal Year: 2013
    ..Results from these studies will provide valuable insight on whether sustained enhancement of myofilament contractility (with dATP) has potential for treatment of heart failure in animal models and humans. ..
  29. Impact of AMP-activated kinase on sex differences in hypertrophic cardiomyopathy
    John P Konhilas; Fiscal Year: 2013
    ..Therefore, it is of major clinical significance that the mechanistic link be determined between cardiac disease, sex/gender differences and energetic regulators in the heart. ) ..
  30. Regeneration of stereocilia of cochlear hair cells
    David Z Z He; Fiscal Year: 2010
    ....
  31. Physiological Regulation of MLCK in Intact Arteries
    Withrow Gil Wier; Fiscal Year: 2013
    ..This research will utilize a mouse model of salt-induced hypertension to provide specific new information on the role of MLCK in high blood pressure. ..
  32. The Role of Myosin in Vesicle Trafficking
    Yashomati M Patel; Fiscal Year: 2010
    ..The proposed research will provide critical information for understanding the molecular mechanism(s) involved in the development of in type 2 diabetes. ..
  33. Molecular mechanics of mutant cardiac myosin
    Jeffrey R Moore; Fiscal Year: 2013
    ..This study will examine how FHC mutations in one of the proteins of the heart (the myosin regulatory light chain), alters the ability of the heart to generate force and power at the molecular level. ..
  34. Development of Inhibitors of the Leukemia Fusion Protein CBFbeta-SMMHC
    Roger A Rajewski; Fiscal Year: 2013
    ..These inhibitors also have the potential to be less toxic than standard chemotherapy, resulting in reduced side effects from treatment. ..
  35. Rho Kinase and Airway Hyperresponsiveness
    Stephanie A Shore; Fiscal Year: 2013
    ..Thus, the ROCK pathway may be an important and still relatively unexplored therapeutic target in asthma. Understanding the role of ROCKs in mouse models of asthma could lead to new strategies for preventing this disease. ..
  36. Nitrergic Neuro-smooth Muscle Transmission in the Gut
    Raj K Goyal; Fiscal Year: 2013
    ..This proposal is to understand how these disorders are caused and how better treatments for these disorders can be developed. ..
  37. Molecular Control of Gut Permeability in Trauma
    Mack H Wu; Fiscal Year: 2013
    ..The research work has potential impact on the development of surgical or medical interventions against gut barrier injury in VA patients with trauma or inflammatory diseases. ..
  38. Contributions of Cardiac Myosin Binding Protein-C to Healthy and Failing Hearts
    CARL WEI CHAN TONG; Fiscal Year: 2013
    ..Thus, funding this proposal will help to solve a vexing health problem and enable a beginning clinician scientist toward path of independent research. ..
  39. Visualizing Actomyosin Transients by Data Merging
    Thomas P Burghardt; Fiscal Year: 2013
    ..These analytical tools are next-generation methods addressing transduction and motility in muscle myosin. ..
  40. Enhancing Energy Expending Adipocytes in White Adipose Tissue
    STEPHEN ROBERT FARMER; Fiscal Year: 2013
    ....
  41. Glycosyltransferase Golgi Retention Mechanism
    Pi Wan Cheng; Fiscal Year: 2013
    ..The results will expand our fundamental understanding of the mechanism of intracellular trafficking and Golgi retention of glycosyltransferases and could help develop therapeutic interventions for these two lung diseases in veterans. ..
  42. Specification of actomyosin function in the cell
    Matthew Lord; Fiscal Year: 2013
    ..Our use of fission yeast (with 1 Tm isoform and 5 myosins total) will overcome this complexity and provide new and novel insights into the specification of actomyosin ..
  43. GENETICS OF HERPESVIRUS TRANSFORMATION
    David M Knipe; Fiscal Year: 2013
    ..Each of these important cell biological problems will be investigated in this proposed research. New antiviral targets for inhibition of herpesviral replication may also be elucidated. ..
  44. Mechanotransduction in the Ciliary Muscle
    Andrew D Pucker; Fiscal Year: 2013
    ..This K08-supported research will form the basis for a future R01 grant application for independent research aimed at creating targeted interventions for treating and preventing ciliary muscle related myopic growth. ..
  45. Cellular and Biochemical Studies of Myosin Assembly in Dictyostelium
    Thomas Egelhoff; Fiscal Year: 2009
    ..The studies we propose addressing the full complement of Dictyostelium alpha kinases will also provide a powerful platform for comparisons and insights into possible roles of alpha kinases in mammalian systems. ..
  46. Myosin ELC, a novel therapeutic target for FHC
    Danuta Szczesna-Cordary; Fiscal Year: 2013
    ..These studies will help to determine the role(s) of the ELC and the N-terminal cardiac specific ELC extension in the regulation of contractile force in healthy and FHC-diseased hearts. ..
  47. MICROFILAMENTS IN THE YEAST SACCHAROMYCES CEREVISIAE
    Anthony P Bretscher; Fiscal Year: 2013
    ..generally mediated by microtubule-based motors, and short range transport and capture by the actin-based motors, myosins. The myosin-V family of molecular motors is evolutionarily highly conserved between animals, plants and fungi, ..
  48. Functional Consequences of FHC-linked RLC Mutations.
    Danuta Szczesna-Cordary; Fiscal Year: 2012
    ....
  49. LISTERIA AND SHIGELLA USE ACTIN TO SPREAD CELL TO CELL
    Frederick Southwick; Fiscal Year: 2013
    ..These discoveries can be applied to all forms of cell motility, including tumor metastasis, neurite outgrowth, and platelet spreading. ..
  50. Interatction of Estrogen, Age and Activity on Musculoskeletal Strength in Females
    Dawn A Lowe; Fiscal Year: 2013
    ..The research described in this application will determine how estradiol treatment can benefit estrogen-deficient females by improving muscle strength. ..
  51. Design and Implementation of Genetically Encoded Myosin Based Force Sensors
    DANIEL PETER KIEHART; Fiscal Year: 2013
    ..Because myosins play key roles in a variety of processes fundamental to human homeostasis and development, we expect these force ..
  52. Cardiac Myosin Binding Protein-C: Structure and Function
    Sakthivel Sadayappan; Fiscal Year: 2013
    ..abstract_text> ..
  53. Axial Flow Effects in Proximal Tubule
    TONG WANG WANG; Fiscal Year: 2012
    ..model predictions with flow-induced changes on Na+ and HCO3- absorption in wild-type, and villin, fimbrin, myosins and cilia-deficient mice;2) To study the role of flow-induced actin cytoskeletal reorganization in modulating ..
  54. Structural Dynamics of Cardiac Muscle Contraction
    Brett A Colson; Fiscal Year: 2012
    ..Since defects in this protein cause human heart disease, this research is likely to have substantial impact on therapeutic approaches to heart disease. ..
  55. Ion Channel Function in Auditory &Vestibular Hair Cells
    Jeffrey R Holt; Fiscal Year: 2013
    ..We expect that this project will produce information that will form the basis for development of future strategies to treat deafness and balance problems in some of the ~28 million Americans who suffer from inner ear disorders. ..
  56. Calcium-dependent Regulation of Smooth Muscle Phenotype
    BRIAN WAMHOFF; Fiscal Year: 2010
    ....
  57. Signal transduction mechanisms to myosin phosphatase
    James T Stull; Fiscal Year: 2013
    ..We will test the hypothesis that the Ca2+-independent kinase ZIPK phosphorylates MYPT1 and CPI-17 as an integral component of the Ca2+-sensitization process. ..
  58. Mechanism of Thin Filament Regulation of Cardiac Actomyosin Hydrolysis
    Howard White; Fiscal Year: 2009
    ..The combined data will be used to determine the mechanism of regulation by cardiac thin filaments and increase our understanding of mechanism of regulation of cardiac contractility. ..
  59. Quantitative Analysis of Chemotactic Motility Cycle of Ameboid Cells
    JUAN CARLOS LASHERAS; Fiscal Year: 2013
    ....
  60. Genetics and Molecular Biology of Striated Muscle Myosin
    Sanford I Bernstein; Fiscal Year: 2013
    ..Overall our project will take advantage of an innovative experimental system to test significant questions regarding basic myosin function and disease. ..
  61. Structure and mechanism of the dynein motor
    Ronald D Vale; Fiscal Year: 2013
    ....
  62. HTS ASSAYS FOR INHIBITORS OF PROTEIN INTERACTIONS INCLUDING MLCK1-FKBP8
    Jerrold R Turner; Fiscal Year: 2012
    ..In addition, the TR-FRET approach will yield robust high throughout screens to identify agents that modulate a wide variety of PPIs in live cells. ..
  63. Cytoskeletal Pliability within Cells under Stress: RBCs and Marrow-derived SCs
    Dennis E Discher; Fiscal Year: 2012
    ..New therapeutic strategies that could benefit more broadly include exon skipping strategies that are presently being applied to the spectrin family protein dystrophin [Goyenvalle Science 2006]. ..
  64. Molecular roles in active and passive mechanics in cochlear hair bundles
    ANTHONY WEI PENG; Fiscal Year: 2013
    ..The technology developed will greatly enhance auditory research and likely have broader mechanics applications in the auditory field and beyond. ..
  65. Regulation of Cytokinesis and Tumor Formation by RhoA
    ANN LOUISE MILLER; Fiscal Year: 2013
    ..Finally, I will test whether cytokinesis failure induced by other Rho zone regulators, especially those that are up- or down-regulated or mutated in human tumors, promotes tumor formation. ..
  66. Signaling pathways that mediate mammalian oocyte cortical mechanics
    Douglas N Robinson; Fiscal Year: 2013
    ..This project will extend understanding of the female gamete in a completely new direction - bringing insights from cellular mechanics into our view of the oocyte-to-egg and egg-to-embryo transitions. ..
  67. Regulation of Endothelial Cell Branching Morphogenesis via MCAK-targted Control
    KENNETH ALBERT MYERS; Fiscal Year: 2013
    ....
  68. Biophysical Mechanisms of Drosophila Development.
    Jennifer A Zallen; Fiscal Year: 2013
    ....
  69. Chromatin mechanism of molecular motor gene regulation
    Ching Pin Chang; Fiscal Year: 2013
    ....
  70. Molecular characteristics of the apical recycling system
    JAMES RICHARD GOLDENRING; Fiscal Year: 2013
    ....
  71. Brain Pericyte and Amyloid-beta Peptide Interaction
    Donghui Zhu; Fiscal Year: 2013
    ..Using in vitro capillary-like structures, we will idenify the rate-limiting step and key-signaling molecules in pericyte adhesion and migration affected by A[unreadable]. The rate-limiting step would be the ideal target for therapies. ..
  72. Estrogenic Effects on Aged Muscle
    Dawn A Lowe; Fiscal Year: 2013
    ..The applicant will conduct research to determine how estradiol treatment can benefit estrogen-deficient females by improving muscle strength. ..
  73. Mutations in Smooth Muscle Contractile Proteins: Pathways to Vascular Diseases
    Dianna M Milewicz; Fiscal Year: 2013
    ..Project 4 will Investigate SMC cellular pathways leading to thoracic aortic disease and SMC proliferation in these same mouse models. ..
  74. Role of Nucleo-cytoskeleton Interactions in Cell Migration
    Gregg G Gundersen; Fiscal Year: 2013
    ....
  75. Mechanism and Prevention of Female Stress Urinary Incontinence
    Tom F Lue; Fiscal Year: 2013
    ..This grant proposal will study means to stop birth-trauma mediated disruption of collagen and elastic fibers before it takes place and/or enhance repair of these fibers. ..
  76. PKD Isoforms in Heart
    Susan F Steinberg; Fiscal Year: 2013
    ..The overarching goal of these studies is to identify novel regulatory controls and cardiac actions of PKDs that can be targeted to prevent or slow the evolution of cardiac hypertrophy and heart failure phenotypes. ..
  77. Functional Relevance of CFTR Trafficking in Vivo to Intestinal Disease
    Nadia A Ameen; Fiscal Year: 2013
    ....
  78. Phosphorylation of cMyBP-C Modulates Cardiac Arrhythmias
    Ramzi J Khairallah; Fiscal Year: 2013
    ..For the PI, the investigation nicely supports his long-term plan of conducting interdisciplinary research related to cardiac function, with the prospect of broadening our understanding of the pathogenesis of heart failure. ..
  79. Functional crosstalk between myosin II &cofilin in regulation of neuronal growth
    Paul Forscher; Fiscal Year: 2013
    ..These studies are predicted to have significant clinical implications for understanding neurodegenerative disease and nerve regeneration related to brain and/or spinal cord injury. ..
  80. TNF-alpha Regulation of Intestinal Paracellular Transport
    Thomas Y Ma; Fiscal Year: 2013
    ..abstract_text> ..
  81. EM STUDIES ON MUSCLE
    KENNETH ALLEN TAYLOR; Fiscal Year: 2013
    ..abstract_text> ..
  82. Using micropost arrays to measure traction forces during dendritic cell motility
    Daniel A Hammer; Fiscal Year: 2013
    ..abstract_text> ..
  83. Mechanisms of Cytokinesis in Yeast
    Erfei Bi; Fiscal Year: 2013
    ..structure with a "kink" in the middle of its tail, bearing all the major features of non-muscle type-II myosins in animal cells...
  84. Dynamic limits on contraction of gastrointestinal smooth muscle
    MARION JOYCE SIEGMAN; Fiscal Year: 2013
    ....
  85. Nonmuscle myosins in craniofacial morphogenesis and orofacial clefting
    JEFFREY OHMANN BUSH; Fiscal Year: 2013
    ..on our preliminary data, combined with recently published human genetics studies, we believe that non-muscle myosins are excellent candidate drivers of craniofacial morphogenesis...
  86. Inflammation, miRNA and autophagy in diabetes
    Paras Kumar Mishra; Fiscal Year: 2013
    ..At translational level, the proposal will provide concrete evidence that over expression of miR-133 or ablation of MMP9 can ameliorate diabetic cardiomyopathy. ..
  87. Thick and Thin Filament Mutations that cause Distal Arthrogryposis
    ALICE WARD RACCA; Fiscal Year: 2013
    ....
  88. COMPUTER SIMULATION THEORY OF GLOBULAR PROTEIN DYNAMICS
    Jeffrey Skolnick; Fiscal Year: 2013
    ..Each Aim is part of a more comprehensive objective to increase our understanding of subcellular processes and biochemical function that will help accelerate drug discovery as well as provide general biochemical insights. ..
  89. Polarity-Mediated Suppression of Tumorigenesis
    GREGORY VINCENT SCHIMIZZI; Fiscal Year: 2013
    ..These advances will further illustrate how the loss of cell polarity contributes to tumor development and progression. ..
  90. Myo1c Participates in Podocyte Junction Formation Through Interaction with Neph1
    Deepak Nihalani; Fiscal Year: 2013
    ....
  91. Atomic resolution structure and function of the ribosome
    JAMIE H CATE; Fiscal Year: 2013
    ..regard, the ribosome is no different from other molecular machines, such as RNA and DNA polymerases, helicases, myosins and kinesins. All of these molecular machines use conformational changes as a basis for function...
  92. Genetic Modification of Cardiac Motor Proteins in Normal and Failing Myocytes
    Eric J Devaney; Fiscal Year: 2013
    ..RELEVANCE (See instructions): The overall goal of the application is to assess the results of manipulation of a cardiac motor protein both to induce cardiac dysfunction and to treat it as well. (End of Abstract) ..
  93. Mechanics of lamellipodial stability, turning and self-polarization
    Alexander Mogilner; Fiscal Year: 2013
    ..This work will result in advanced understanding of cell motility, and will also produce broadly applicable novel mathematical tools as well as mathematical model components that can be integrated with existing models of cell migration. ..
  94. Targeted Rho kinase-1 KO Mouse Model for Vascular Smooth Muscle Remodeling
    DENIZ TOKSOZ-EXLEY; Fiscal Year: 2013
    ..Overall, the Aims will generate and characterize a novel genetic ROCK animal model, and test the hypothesis in vivo that ROCK1 contributes to remodeling and pulmonary hypertensive disease. ..
  95. CYTOSKELETAL MECHANISMS OF RETINAL DEVELOPMENT
    Donald F Ready; Fiscal Year: 2013
    ..If the aims of this project are achieved, key properties of core cell functions will be established in this model. ..
  96. Structural Dynamics of Stretch Activation in Muscle
    MICHAEL KAY REEDY; Fiscal Year: 2013
    ....
  97. Allostery in myosins studied at the molecular level
    Ronald S Rock; Fiscal Year: 2013
    ..In prior studies, we discovered two myosins with new and distinct processive stepping patterns on actin...
  98. Molecular mechanisms of cell shape change in cytokinesis
    Amy Shaub Maddox; Fiscal Year: 2013
    ..In addition, because currently used anti-mitotics also have limited success against some tumor types, development of cytokinesis drugs will be a welcome expansion and diversification of our arsenal against cancers. ..
  99. Role of the Inflammatory Cytokine Oncostatin-M in Promoting Atherosclerosis
    Errol Wijelath; Fiscal Year: 2013
    ..This proposed work will elucidate the role of an important biological factor, OSM, in the evolution of the atherosclerosis. It will also identify STAT-1 pathway as a therapeutic target for controlling pathological vascular remodeling. ..
  100. A novel autophagy gene beclin 2 in the prevention of type 2 diabetes and obesity
    Congcong He; Fiscal Year: 2013
    ....
  101. Molecular Characterization of Cardiomyopathy Mutations in Human Cardiac Myosin
    JAMES ANTHONY SPUDICH; Fiscal Year: 2013
    ..Most studies to determine the functional impact of these mutations on myosin have used non-human myosins and most have not used cardiac myosins...