Genomes and Genes
Summary: A diverse superfamily of proteins that function as translocating proteins. They share the common characteristics of being able to bind ACTINS and hydrolyse MgATP. Myosins generally consist of heavy chains which are involved in locomotion, and light chains which are involved in regulation. Within the structure of myosin heavy chain are three domains: the head, the neck and the tail. The head region of the heavy chain contains the actin binding domain and MgATPase domain which provides energy for locomotion. The neck region is involved in binding the light-chains. The tail region provides the anchoring point that maintains the position of the heavy chain. The superfamily of myosins is organized into structural classes based upon the type and arrangement of the subunits they contain.
Publications376 found, 100 shown here
- Contraction and polymerization cooperate to assemble and close actomyosin rings around Xenopus oocyte woundsC A Mandato
Department of Zoology, University of Wisconsin, Madison, WI 53706, USA
J Cell Biol 154:785-97. 2001....
- Recombinant motor domain constructs of Chara corallina myosin display fast motility and high ATPase activityKohji Ito
Department of Biology, Chiba University, Inage Ku, 263 8522, Chiba, Japan
Biochem Biophys Res Commun 312:958-64. 2003..Our results provide an excellent system to dissect the specific structural and functional features that distinguish the myosin responsible for fast cytoplasmic streaming...
- Myosin-dependent endoplasmic reticulum motility and F-actin organization in plant cellsHaruko Ueda
Department of Botany, Graduate School of Science, Kyoto University, Kyoto 606 8502, Japan
Proc Natl Acad Sci U S A 107:6894-9. 2010..Although it was long assumed that the ER dynamics is actomyosin-driven, the responsible myosins were not identified, and the ER streaming was not characterized quantitatively...
- Drawing the tree of eukaryotic life based on the analysis of 2,269 manually annotated myosins from 328 speciesFlorian Odronitz
Department of NMR Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg, 37077 Goettingen, Germany
Genome Biol 8:R196. 2007..Incongruence between phylogenetic trees can result from the violation of the orthology assumption and stochastic and systematic errors...
- Analysis of the actin-myosin II system in fish epidermal keratocytes: mechanism of cell body translocationT M Svitkina
Laboratory of Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
J Cell Biol 139:397-415. 1997..We propose that the forward translocation of the cell body and retrograde flow in the lamellipodia are both driven by contraction of an actin-myosin network in the lamellipodial/cell body transition zone...
- Involvement of an actomyosin contractile ring in Saccharomyces cerevisiae cytokinesisE Bi
Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
J Cell Biol 142:1301-12. 1998..cerevisiae. In its absence, cytokinesis can still be completed by a process (possibly localized cell-wall synthesis leading to septum formation) that appears to require septin function and to be facilitated by F-actin...
- Myosins: a diverse superfamilyJ R Sellers
National Heart, Lung and Blood Institute, National Institutes of Health, Building 10, Room 8N202, Bethesda, MD 20892, USA
Biochim Biophys Acta 1496:3-22. 2000b>Myosins constitute a large superfamily of actin-dependent molecular motors. Phylogenetic analysis currently places myosins into 15 classes. The conventional myosins which form filaments in muscle and non-muscle cells form class II...
- Diaphanous regulates myosin and adherens junctions to control cell contractility and protrusive behavior during morphogenesisCatarina C F Homem
Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3280, USA
Development 135:1005-18. 2008..These effects also are mediated through coordinated effects on myosin activity and adhesion, suggesting a common mechanism for Diaphanous action during morphogenesis...
- folded gastrulation, cell shape change and the control of myosin localizationRachel E Dawes-Hoang
Department of Molecular Biology, Howard Hughes Medical Institute, Princeton University, NJ 08544, USA
Development 132:4165-78. 2005..Our analysis of fog function therefore provides a direct link from patterning to cell shape change...
- Targeted genomic capture and massively parallel sequencing to identify genes for hereditary hearing loss in Middle Eastern familiesZippora Brownstein
Department of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
Genome Biol 12:R89. 2011..Our subjects for genome analysis are Israeli Jewish and Palestinian Arab families with hearing loss that varies in mode of inheritance and severity...
- Molecular basis of human Usher syndrome: deciphering the meshes of the Usher protein network provides insights into the pathomechanisms of the Usher diseaseJan Reiners
Institute of Zoology, Department of Cell and Matrix Biology, Johannes Gutenberg University of Mainz, Müllerweg 6, D 55099 Mainz, Germany
Exp Eye Res 83:97-119. 2006....
- New insights into myosin evolution and classificationBernardo J Foth
Department of Microbiology and Molecular Medicine, Centre Medical Universitaire, University of Geneva, 1 rue Michel Servet, 1211 Geneva, Switzerland
Proc Natl Acad Sci U S A 103:3681-6. 2006b>Myosins are eukaryotic actin-dependent molecular motors important for a broad range of functions like muscle contraction, vision, hearing, cell motility, and host cell invasion of apicomplexan parasites...
- The many different cellular functions of MYO7A in the retinaDavid S Williams
Department of Neurobiology and Ophthalmology, UCLA School of Medicine, Los Angeles, CA 90024, USA
Biochem Soc Trans 39:1207-10. 2011..It is likely that the progressive retinal degeneration that occurs in Usher syndrome 1B patients results from a combination of cellular defects in the RPE and photoreceptor cells...
- An actin-myosin complex on actively transcribing genesNathalie Fomproix
Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institute, Stockholm 171 77, Sweden
Exp Cell Res 294:140-8. 2004....
- Mechanism of force generation by myosin heads in skeletal muscleGabriella Piazzesi
Universita di Firenze, Viale G B Morgagni 63, I 50134 Firenze, Italy
Nature 415:659-62. 2002....
- Myosin VIIa and sans localization at stereocilia upper tip-link density implicates these Usher syndrome proteins in mechanotransductionM Hamed Grati
Laboratory of Cell Structure and Dynamics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 108:11476-81. 2011..We propose that MYO7A, sans, and harmonin-b form the core components of the UTLD molecular complex. In this complex, MYO7A is likely the motor element that pulls on CDH23 to exert tension on the tip-link...
- Specific nuclear localizing sequence directs two myosin isoforms to the cell nucleus in calmodulin-sensitive mannerRastislav Dzijak
Department of Biology of the Cell Nucleus, Institute of Molecular Genetics of the ASCR, v v i, Prague, Czech Republic
PLoS ONE 7:e30529. 2012..NM1 differs from the "cytoplasmic" myosin 1c only by additional 16 amino acids at the N-terminus of the molecule. This amino acid stretch was therefore suggested to direct NM1 into the nucleus...
- ROCK and Dia have opposing effects on adherens junctions downstream of RhoErik Sahai
Cancer Research UK Centre for Cell and Molecular Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK
Nat Cell Biol 4:408-15. 2002..The ability of Dia1 to regulate the actin network is crucial for the localization of adherens junction components to the cell periphery...
- Identification of the membrane receptor of a class XIV myosin in Toxoplasma gondiiElizabeth Gaskins
Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, 108 Taylor Hall, CB 7090, Chapel Hill, NC 27599, USA
J Cell Biol 165:383-93. 2004..These results provide the first molecular description of an integral membrane protein as a specific receptor for a myosin motor, and further our understanding of the motile apparatus underlying gliding motility in apicomplexan parasites...
- Cortical recruitment of nonmuscle myosin II in early syncytial Drosophila embryos: its role in nuclear axial expansion and its regulation by Cdc2 activityAnne Royou
Centre National de la Recherche Scientifique, Centre de Genetique Moleculaire, 91198 Gif sur Yvette, France
J Cell Biol 158:127-37. 2002..These studies suggest a model in which cycles of myosin-mediated contraction and relaxation, tightly linked to Cdc2 and Rho kinase activity, are directly responsible for the axial expansion of the syncytial nuclei...
- Abnormal phagocytosis by retinal pigmented epithelium that lacks myosin VIIa, the Usher syndrome 1B proteinDaniel Gibbs
Department of Pharmacology, School of Medicine, University of California at San Diego, La Jolla 92093 0912, USA
Proc Natl Acad Sci U S A 100:6481-6. 2003..Because the phagocytosis of photoreceptor disks by the RPE has been shown to be critical for photoreceptor cell viability, this defect likely contributes to the progressive blindness in USH1B...
- Class XI myosins are required for development, cell expansion, and F-Actin organization in ArabidopsisValera V Peremyslov
Department of Botany and Plant Pathology and Center for Genome Research and Biocomputing, Oregon State University, Corvallis, Oregon 97331, USA
Plant Cell 22:1883-97. 2010..Although F-actin is essential for cell growth and plant development, roles of the associated myosins are poorly understood...
- A comparative study of the involvement of 17 Arabidopsis myosin family members on the motility of Golgi and other organellesDror Avisar
Institute of Plant Sciences, Volcani Center, Bet Dagan 50250, Israel
Plant Physiol 150:700-9. 2009..Truncated fragments of all 17 annotated Arabidopsis myosins containing either the IQ tail or tail domains only were fused to fluorescent markers and coexpressed with a Golgi ..
- Phosphorylation and activation of myosin by Rho-associated kinase (Rho-kinase)M Amano
Division of Signal Transduction, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma 630 01
J Biol Chem 271:20246-9. 1996..The activated Rho-kinase subsequently phosphorylates MLC. This may partly account for the mechanism by which Rho regulates cytokinesis, cell motility, or smooth muscle contraction...
- Mutant myosin VIIa causes defective melanosome distribution in the RPE of shaker-1 miceX Liu
Nat Genet 19:117-8. 1998
- Unconventional myosins acting unconventionallySarah Woolner
Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester, UK
Trends Cell Biol 19:245-52. 2009Unconventional myosins are proteins that bind actin filaments in an ATP-regulated manner...
- Planar polarization of the atypical myosin Dachs orients cell divisions in DrosophilaYanlan Mao
Apoptosis and Proliferation Control Laboratory, Cancer Research UK, London Research Institute, London WC2A 3PX, United Kingdom
Genes Dev 25:131-6. 2011..Planar polarization of Dachs is ultimately oriented by long-range gradients emanating from compartment boundaries, and is therefore a mechanism linking these gradients with the control of tissue shape...
- Role of cortical tension in bleb growthJean Yves Tinevez
Max Planck Institute of Molecular Cell Biology and Genetics, 01 307 Dresden, Germany
Proc Natl Acad Sci U S A 106:18581-6. 2009..Together, our observations and model allow us to relate bleb shape parameters to the underlying cellular mechanics and provide insights as to how bleb formation can be biochemically regulated during cell motility...
- A Role of myosin Vb and Rab11-FIP2 in the aquaporin-2 shuttlePavel I Nedvetsky
Leibniz Institut fur Molekulare Pharmakologie FMP, Campus Berlin Buch, 13125 Berlin, Germany
Traffic 8:110-23. 2007..In summary, we identified myosin Vb as a motor protein involved in AQP2 recycling and show that myosin Vb- and Rab11-FIP2-dependent recycling of AQP2 is an integral part of the AQP2 shuttle...
- Differing requirements for actin and myosin by plant viruses for sustained intercellular movementPhillip A Harries
Plant Biology Division, The Samuel Roberts Noble Foundation, Inc 2510 Sam Noble Parkway, Ardmore, OK 73401, USA
Proc Natl Acad Sci U S A 106:17594-9. 2009..Here we determine the importance of microfilaments and myosins for the sustained intercellular movement of a group of RNA-based plant viruses...
- The ubiquitin-selective chaperone CDC-48/p97 links myosin assembly to human myopathyPhilipp Christoph Janiesch
Centre for Molecular Neurobiology ZMNH, University of Hamburg, Falkenried 94, 20251 Hamburg, Germany
Nat Cell Biol 9:379-90. 2007....
- RhoA is required for monocyte tail retraction during transendothelial migrationR A Worthylake
Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
J Cell Biol 154:147-60. 2001..Finally, we find that p160ROCK signaling negatively regulates integrin adhesions and that inhibition of RhoA results in an accumulation of beta2 integrin in the unretracted tails...
- Chaperone-mediated folding and assembly of myosin in striated muscleRajani Srikakulam
Department of Pathology and Laboratory Medicine, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, NJ 08854, USA
J Cell Sci 117:641-52. 2004..We conclude that multimeric complexes of nascent myosin filaments associated with Hsc70 and Hsp90 are intermediates in the folding and assembly pathway of muscle myosin...
- Human myosin-Vc is a novel class V myosin expressed in epithelial cellsOlga C Rodriguez
Department of Cell and Molecular Physiology, Medical Science Research Building, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
J Cell Sci 115:991-1004. 2002Class V myosins are one of the most ancient and widely distributed groups of the myosin superfamily and are hypothesized to function as motors for actin-dependent organelle transport...
- The unconventional myosin-VIIa associates with lysosomesLily E Soni
Section of Cell and Developmental Biology, Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093 0368, USA
Cell Motil Cytoskeleton 62:13-26. 2005..Myosin-VIIa copurified with lysosomes on density gradients, and fractionation and extraction experiments suggested that it was tightly associated with the lysosome surface. These studies suggest that myosin-VIIa is a lysosome motor...
- Utilization of myosin and actin bundles for the transport of molecular cargoHideyo Takatsuki
Laboratory of Molecular Physiology, Robert C Byrd Biotechnology Science Center, Department of Biological Sciences, Marshall University, Huntington, WV 25755 1090, USA
Small 6:452-7. 2010..These data suggest that actin bundling may significantly improve the applicability of the myosin motor for future nanotechnological applications...
- Functional characterization of the human myosin-7a motor domainSarah M Heissler
Institut für Biophysikalische Chemie, Medizinische Hochschule Hannover, Hannover, Germany
Cell Mol Life Sci 69:299-311. 2012..Our results suggest that in a cellular environment, compartment-specific fluctuations in free Mg(2+) ions can mediate the conditional switching of myosin-7a between cargo moving and tension bearing modes...
- A myosin XI tail domain homologous to the yeast myosin vacuole-binding domain interacts with plastids and stromules in Nicotiana benthamianaAmir Sattarzadeh
Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
Mol Plant 2:1351-8. 2009..The involvement of myosins in chloroplast positioning and mitochondrial movement was demonstrated by expression of a virus-induced gene ..
- Build it up-Tear it down: protein quality control in the cardiac sarcomereMonte S Willis
Carolina Cardiovascular Biology Center, University of North Carolina, 8200 Medical Biomolecular Research Bldg, 103 Mason Farm Road, Chapel Hill, NC 27599 7126, USA
Cardiovasc Res 81:439-48. 2009..In this review, we highlight the dynamic interplay between sarcomere-specific chaperones and ubiquitin-dependent degradation of sarcomere proteins that is necessary in order to maintain structure and function of the cardiac sarcomere...
- Nuclear actin and myosins: life without filamentsPrimal de Lanerolle
Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, Illinois 60612, USA
Nat Cell Biol 13:1282-8. 2011..were traditionally thought to function only in the cytoplasm, it is now well accepted that actin and multiple myosins are found in the nucleus...
- Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1K Ebnet
Institute of Cell Biology, ZMBE, University of Muenster, D 48149 Muenster, Germany
J Biol Chem 275:27979-88. 2000..Our results suggest that JAM can be recruited to intercellular junctions by its interaction with the PDZ domain-containing proteins AF-6 and possibly ZO-1...
- Myosin vb is associated with plasma membrane recycling systemsL A Lapierre
Departments of Medicine, Surgery, and Cellular Biology and Anatomy, Institute of Molecular Medicine and Genetics, Medical College of Georgia and the Augusta VA Medical Center, Augusta, Georgia 30912, USA
Mol Biol Cell 12:1843-57. 2001..The dominant negative effects of the myosin Vb tail chimera indicate that this unconventional myosin is required for transit out of plasma membrane recycling systems...
- Sequential assembly of myosin II, an IQGAP-like protein, and filamentous actin to a ring structure involved in budding yeast cytokinesisJ Lippincott
Department of Cell Biology, Harvard Medical School, Boston, Massachussetts 02115, USA
J Cell Biol 140:355-66. 1998....
- Tobacco mosaic virus infection spreads cell to cell as intact replication complexesShigeki Kawakami
Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, MO 63132, USA
Proc Natl Acad Sci U S A 101:6291-6. 2004..We propose a model whereby cell-cell spread of tobamovirus infection is accomplished by subviral replication complexes that initiate TMV replication immediately after entry to adjacent cells...
- Update on Usher syndromeZubin Saihan
UCL Institute of Ophthalmology and Moorfields Eye Hospital, London, UK
Curr Opin Neurol 22:19-27. 2009..The present review addresses the mechanisms, genetics and pathogenesis of Usher syndrome...
- Expression in cochlea and retina of myosin VIIa, the gene product defective in Usher syndrome type 1BT Hasson
Department of Biology, Yale University, New Haven, CT 06520, USA
Proc Natl Acad Sci U S A 92:9815-9. 1995....
- Specificity of blebbistatin, an inhibitor of myosin IIJohn Limouze
Laboratory of Molecular Cardiology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Muscle Res Cell Motil 25:337-41. 2004..Blebbistatin potently inhibits several striated muscle myosins as well as vertebrate nonmuscle myosin IIA and IIB with IC50 values ranging from 0.5 to 5 microM...
- Afadin: A novel actin filament-binding protein with one PDZ domain localized at cadherin-based cell-to-cell adherens junctionK Mandai
Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c o JCR Pharmaceuticals Co, Ltd, Kobe 651 22, Japan
J Cell Biol 139:517-28. 1997..These results suggest that l-afadin serves as a linker of the actin cytoskeleton to the plasma membrane at cell-to-cell AJ...
- A high precision survey of the molecular dynamics of mammalian clathrin-mediated endocytosisMarcus J Taylor
Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom
PLoS Biol 9:e1000604. 2011....
- Functional analysis of the Drosophila diaphanous FH protein in early embryonic developmentK Afshar
Department of Biology, Center for Molecular Genetics, University of California, San Diego, La Jolla CA 92093 0634, USA
Development 127:1887-97. 2000..Based on known biochemical functions of FH proteins, we propose that Diaphanous serves as a mediator between signaling molecules and actin organizers at specific phases of the cell cycle...
- Phosphorylation switches specific for the cardiac isoform of myosin binding protein-C: a modulator of cardiac contraction?M Gautel
European Molecular Biology Laboratory, Heidelberg, Germany
EMBO J 14:1952-60. 1995..2 in humans, and is therefore in a region of physical linkage to subsets of familial hypertrophic cardiomyopathy (FHC). This makes cardiac MyBP-C a candidate gene for chromosome 11-associated FHC...
- Class VIII myosins are required for plasmodesmatal localization of a closterovirus Hsp70 homologDror Avisar
Department of Botany and Plant Pathology, Oregon State University, Cordley Hall 2082, Corvallis, OR 97331, USA
J Virol 82:2836-43. 2008..I. Prokhnevsky, V. V. Peremyslov, and V. V. Dolja, J. Virol. 79:14421-14428, 2005). Myosins are a diverse category of molecular motors that possess a motor domain and a tail domain involved in cargo ..
- Myosin XI is essential for tip growth in Physcomitrella patensLuis Vidali
Department of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, Massachusetts 01609, USA
Plant Cell 22:1868-82. 2010Class XI myosins are plant specific and responsible for cytoplasmic streaming. Because of the large number of myosin XI genes in angiosperms, it has been difficult to determine their precise role, particularly with respect to tip growth...
- Dynamics and regulation of contractile actin-myosin networks in morphogenesisKaren E Kasza
Howard Hughes Medical Institute, Developmental Biology Program, Sloan Kettering Institute, 1275 York Avenue, New York, NY 10065, USA
Curr Opin Cell Biol 23:30-8. 2011..Analysis of the complex behaviors of actin-myosin networks will be crucial for understanding force generation in actively remodeling cells and the coordination of cell shape and movement at the tissue level...
- Expression, splicing, and evolution of the myosin gene family in plantsValera V Peremyslov
Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331, USA
Plant Physiol 155:1191-204. 2011Plants possess two myosin classes, VIII and XI. The myosins XI are implicated in organelle transport, filamentous actin organization, and cell and plant growth...
- In the thick of it: HCM-causing mutations in myosin binding proteins of the thick filamentSamantha P Harris
Department of Neurobiology, Physiology, and Behavior College of Biological Sciences, University of California, One Shields Ave, Davis, CA 95616, USA
Circ Res 108:751-64. 2011..Additional work is still needed to determine the mechanisms by which individual mutations induce hypertrophic phenotypes...
- Reduced climbing and increased slipping adaptation in cochlear hair cells of mice with Myo7a mutationsC J Kros
School of Biological Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK
Nat Neurosci 5:41-7. 2002..We conclude that myosin VIIA participates in anchoring and holding membrane-bound elements to the actin core of the stereocilium. Myosin VIIA is therefore required for the normal gating of transducer channels...
- Myosin XVa localizes to the tips of inner ear sensory cell stereocilia and is essential for staircase formation of the hair bundleInna A Belyantseva
Laboratory of Molecular Genetics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, 5 Research Court, Rockville, MD 20850, USA
Proc Natl Acad Sci U S A 100:13958-63. 2003..We propose that myosin XVa is essential for the graded elongation of stereocilia during their functional maturation...
- Shaker-1 mutations reveal roles for myosin VIIA in both development and function of cochlear hair cellsT Self
MRC Institute of Hearing Research, University Park, Nottingham NG7 2RD, UK
Development 125:557-66. 1998..These findings suggest that myosin VIIA is required for normal stereocilia bundle organisation and has a role in the function of cochlear hair cells...
- Myosin-10 and actin filaments are essential for mitotic spindle functionSarah Woolner
Department of Zoology, University of Wisconsin Madison, Madison, WI 53706, USA
J Cell Biol 182:77-88. 2008..in mitotic spindles are well established, whether or not actin filaments (F-actin) and F-actin-based motors (myosins) are required components of mitotic spindles has long been controversial...
- Movement and remodeling of the endoplasmic reticulum in nondividing cells of tobacco leavesI Sparkes
School of Life Sciences, Oxford Brookes University, Oxford OX3 0BP, United Kingdom
Plant Cell 21:3937-49. 2009..Expression of a subset of truncated class XI myosins, which interfere with the activity of native class XI myosins, and drug-induced actin depolymerization produce a ..
- Myosin Vs organize actin cables in fission yeastLibera Lo Presti
Department of Fundamental Microbiology, University of Lausanne, CH 1015 Lausanne, Switzerland
Mol Biol Cell 23:4579-91. 2012..Together these in vivo data reveal elements of a self-organizing system in which the motors shape their own tracks by transporting cargoes and exerting physical pulling forces...
- Wound-induced assembly and closure of an actomyosin purse string in Xenopus oocytesW M Bement
Department of Zoology, Program in Cellular and Molecular Biology, University of Wisconsin Madison, 1117 West Johnson Street, Madison, Wisconsin 53706, USA
Curr Biol 9:579-87. 1999....
- Functional roles for myosin 1c in cellular signaling pathwaysLisa M Bond
Cambridge Institute for Medical Research, University of Cambridge, Wellcome Trust MRC Building, Hills Road, Cambridge, CB2 0XY, United Kingdom
Cell Signal 25:229-35. 2013..This review provides an overview of the functional involvement of myosin 1c in cellular signaling and discusses the possible potential for myosin 1c as a target for drug-based treatments for human diseases...
- Inhibition of myosin light chain kinase by p21-activated kinaseL C Sanders
Departments of Immunology and Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Science 283:2083-5. 1999..These data indicate that MLCK is a target for PAKs and that PAKs may regulate cytoskeletal dynamics by decreasing MLCK activity and MLC phosphorylation...
- Kinetic and equilibrium analysis of the myosin ATPaseEnrique M De La Cruz
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, USA
Methods Enzymol 455:157-92. 2009..ATPase pathway vary significantly across the myosin superfamily, resulting in kinetic differences that that allow myosins to carry out diverse mechanical functions...
- Missense mutations in the beta-myosin heavy-chain gene cause central core disease in hypertrophic cardiomyopathyL Fananapazir
Cardiology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892
Proc Natl Acad Sci U S A 90:3993-7. 1993..This work demonstrates that (i) MYH7-associated HCM is often a disease of striated muscle but with predominant cardiac involvement and (ii) a subset of HCM patients with MYH7 gene missense mutations have CCD...
- Chromatin remodelling and transcription: be-WICHed by nuclear myosin 1Piergiorgio Percipalle
Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institute, P O Box 285, Stockholm SE 171 77, Sweden
Curr Opin Cell Biol 18:267-74. 2006..NoRC produces a repressive chromatin state; B-WICH, together with nuclear myosin 1, activates pol I transcription directly on chromatin templates and might also function in the maintenance of ribosomal chromatin structure...
- Rigor-like structures from muscle myosins reveal key mechanical elements in the transduction pathways of this allosteric motorYuting Yang
Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454, USA
Structure 15:553-64. 2007..The overall arrangements of subdomains in the motor are relatively conserved in each of the known contractile states, and we explore qualitatively the energetics of these states...
- Myosin-X: a MyTH-FERM myosin at the tips of filopodiaMichael L Kerber
Department of Cell and Molecular Physiology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7545, USA
J Cell Sci 124:3733-41. 2011..At the cellular and organismal level, growing evidence demonstrates that Myo10 has crucial functions in numerous processes ranging from invadopodia formation to cell migration...
- Requirement of myosin Vb.Rab11a.Rab11-FIP2 complex in cholesterol-regulated translocation of NPC1L1 to the cell surfaceBei Bei Chu
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China
J Biol Chem 284:22481-90. 2009..These results suggest that the efficient transport of NPC1L1 to the PM is dependent on the microfilament-associated myosin Vb.Rab11a.Rab11-FIP2 triple complex...
- A mechanical model of actin stress fiber formation and substrate elasticity sensing in adherent cellsSam Walcott
Mechanical Engineering and Johns Hopkins Physical Science Oncology Center, Johns Hopkins University, Baltimore, MD 21218, USA
Proc Natl Acad Sci U S A 107:7757-62. 2010..As these large bundles of actin, called stress fibers, appear preferentially on stiff surfaces, our mechanical model provides a mechanism for stress fiber formation and stiffness sensing in cells adhered to a compliant surface...
- Role of phosphatidylinositol 4-phosphate (PI4P) and its binding protein GOLPH3 in hepatitis C virus secretionBryan Bishe
Division of Infectious Diseases, Moores Cancer Center, University of California, San Diego, La Jolla, California 92093, USA
J Biol Chem 287:27637-47. 2012..These studies establish the role of PI4P and its interacting protein GOLPH3 in HCV secretion and strengthen the significance of the Golgi secretory pathway in this process...
- Localization of the rat myosin I molecules myr 1 and myr 2 and in vivo targeting of their tail domainsC Ruppert
Friedrich Miescher Laboratorium der Max Planck Gesellschaft, Tubingen, Germany
J Cell Sci 108:3775-86. 1995..These results suggest that the tail domains of myr 1 and myr 2 are sufficient for subcellular targeting but that their head domains also contribute significantly to maintaining a proper subcellular localization...
- Coupling actin flow, adhesion, and morphology in a computational cell motility modelDanying Shao
Center for Theoretical Biological Physics and Department of Physics, University of California, San Diego, La Jolla, CA 92093 0374, USA
Proc Natl Acad Sci U S A 109:6851-6. 2012..Our model suggests that the pattern of the actin flow inside the cell, the cell velocity, and the cell morphology are determined by the integration of actin polymerization, myosin contraction, adhesion forces, and membrane forces...
- Comparative kinetic and functional characterization of the motor domains of human nonmuscle myosin-2C isoformsSarah M Heissler
Institute for Biophysical Chemistry, Hannover Medical School, 30625 Hannover, Germany
J Biol Chem 286:21191-202. 2011Nonmuscle myosins are widely distributed and play important roles in the maintenance of cell morphology and cytokinesis...
- A novel actin binding site of myosin required for effective muscle contractionBoglárka H Várkuti
Department of Biochemistry, Eotvos Lorand University, Budapest, Hungary
Nat Struct Mol Biol 19:299-306. 2012..We conclude that the binding of actin to myosin's activation loop specifically increases the ratio of mechanically productive to futile myosin heads, leading to efficient muscle contraction...
- Control of actin moving trajectory by patterned poly(methylmethacrylate) tracksH Suzuki
Kansai Advanced Research Center, Communications Research Laboratory, Kobe, Japan
Biophys J 72:1997-2001. 1997..The mean velocity of actin movement on the PMMA was 4.5 mm/s at 25 degrees C, and it was comparable to that on a conventionally used nitrocellulose film...
- Domain structure of a mammalian myosin I betaO Reizes
Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas 75235
Proc Natl Acad Sci U S A 91:6349-53. 1994..5-kDa domain that contains the ATP- and actin-binding sites, followed by an 8.5-kDa domain with three calmodulin-binding sequences and a basic 30-kDa carboxyl-terminal tail segment that binds to anionic phospholipids and membranes...
- Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNAR M Long
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
Science 277:383-7. 1997..A cis-acting element in the 3'-untranslated region of ASH1 mRNA is sufficient to localize a chimeric RNA to daughter cells. These results suggest that localization of mRNA may have been an early property of the eukaryotic lineage...
- Cooperative [Ca²+]-dependent regulation of the rate of myosin binding to actin: solution data and the tropomyosin chain modelMichael Geeves
Department of Biosciences, University of Kent, Canterbury, Kent, United Kingdom
Biophys J 100:2679-87. 2011..Implications for theories of thin-filament regulation in muscle are discussed...
- Deficiency of Myo18B in mice results in embryonic lethality with cardiac myofibrillar aberrationsRieko Ajima
Biology Division, National Cancer Center Research Institute, Tokyo 104 0045, Japan
Genes Cells 13:987-99. 2008Myo18B is an unconventional myosin family protein expressed predominantly in muscle cells. Although conventional myosins are known to be localized on the A-bands and function as a molecular motor for muscle contraction, Myo18B protein ..
- A FERM domain autoregulates Drosophila myosin 7a activityYi Yang
Laboratory of Molecular Physiology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 106:4189-94. 2009..We propose that at low cellular actin concentrations, myosin 7a-FL is bent and inactive, but at high actin concentrations, it is unfolded and active because the C-terminal FERM domain binds to actin...
- Mother cell-specific HO expression in budding yeast depends on the unconventional myosin myo4p and other cytoplasmic proteinsR P Jansen
Research Institute of Molecular Pathology, Vienna, Austria
Cell 84:687-97. 1996..In an accompanying paper, we show that SHE genes are needed for the accumulation in daughter nuclei of Ash1p, a repressor of HO...
- Myosin IXa regulates epithelial differentiation and its deficiency results in hydrocephalusMarouan Abouhamed
Institute of General Zoology and Genetics, Westfalian Wilhelms University, 48149 Munster, Germany
Mol Biol Cell 20:5074-85. 2009..Moreover, Rho-kinases may represent a new target for therapeutic intervention in some forms of hydrocephalus...
- The effect of ageing and immobilization on structure and function of human skeletal muscle fibresGiuseppe D'Antona
Department of Experimental Medicine, Human Physiology Unit, University of Pavia, Pavia, Italy
J Physiol 552:499-511. 2003....
- Characterization of myosin-IA and myosin-IB, two unconventional myosins associated with the Drosophila brush border cytoskeletonN S Morgan
Department of Genetics, School of Medicine, Yale University, New Haven, Connecticut 06520, USA
Dev Biol 172:51-71. 1995The expression patterns of myosin-IA (MIA) and myosin-IB (MIB), two novel unconventional myosins from Drosophila melanogaster, have been characterized through immunoblot analysis and immunocytochemistry of embryos, larvae, and adults...
- A Rictor-Myo1c complex participates in dynamic cortical actin events in 3T3-L1 adipocytesG Nana Hagan
Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
Mol Cell Biol 28:4215-26. 2008..Taken together, our findings suggest that the Rictor-Myo1c complex is distinct from mTORC2 and that Myo1c, in conjunction with Rictor, participates in cortical actin remodeling events...
- Mechanism and specificity of pentachloropseudilin-mediated inhibition of myosin motor activityKrishna Chinthalapudi
Research Centre for Structural Analysis, Hannover Medical School, Hannover, Germany
J Biol Chem 286:29700-8. 2011..IC(50) values are in the range from 1 to 5 μm for mammalian class-1 myosins and greater than 90 μm for class-2 and class-5 myosins, and no inhibition was observed with class-6 and class-7 ..
- Myosin XI-K Is required for rapid trafficking of Golgi stacks, peroxisomes, and mitochondria in leaf cells of Nicotiana benthamianaDror Avisar
Department of Botany and Plant Pathology and Center for Genome Research and Biocomputing, Oregon State University, Corvallis, OR 97331, USA
Plant Physiol 146:1098-108. 2008..We sequenced six complete Nicotiana benthamiana cDNAs that encode class XI and class VIII myosins. Phylogenetic analysis indicates that these two classes of myosins diverged prior to the radiation of green algae ..
- Effects of a R133W beta-tropomyosin mutation on regulation of muscle contraction in single human muscle fibresJulien Ochala
Department of Clinical Neurophysiology, Uppsala University Hospital, Entrance 85, 3rd Floor, SE 751 85 Uppsala, Sweden
J Physiol 581:1283-92. 2007....
- Antibodies covalently immobilized on actin filaments for fast myosin driven analyte transportSaroj Kumar
School of Natural Sciences, Linnaeus University, Kalmar, Sweden
PLoS ONE 7:e46298. 2012....
- Mature mice lacking Rbl2/p130 gene have supernumerary inner ear hair cells and supporting cellsSonia M Rocha-Sanchez
Department of Oral Biology, Creighton University School of Dentistry, Omaha, Nebraska 68178, USA
J Neurosci 31:8883-93. 2011..Interestingly, p130(-/-) mice exhibited nearly normal peripheral auditory sensitivity...
- Myosin Vc is a molecular motor that functions in secretory granule traffickingDamon T Jacobs
Department of Cell and Molecular Physiology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Mol Biol Cell 20:4471-88. 2009Class V myosins are actin-based motor proteins that have critical functions in organelle trafficking...
- Myosin motor Myo1c and its receptor NEMO/IKK-gamma promote TNF-alpha-induced serine307 phosphorylation of IRS-1Yoshitaka Nakamori
Division of Molecular Analysis of Human Disorders, Department of Bio Signal Analysis, Yamaguchi University Graduate School of Medicine, Ube 755 8505, Japan
J Cell Biol 173:665-71. 2006..Thus, motor protein Myo1c and its receptor protein NEMO act cooperatively to form the IKK-IRS-1 complex and function in TNF-alpha-induced insulin resistance...
- Dynamic regulation of sarcomeric actin filaments in striated muscleShoichiro Ono
Department of Pathology and Department of Cell Biology, Emory University, Atlanta, Georgia 30322, USA
Cytoskeleton (Hoboken) 67:677-92. 2010..Further investigation of the regulatory mechanism of actin dynamics in striated muscle should be a key to understanding how myofibrils develop and operate...
- Identification of two type V myosins in fission yeast, one of which functions in polarized cell growth and moves rapidly in the cellF Motegi
Division of Biology, Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Tokyo, 153-8902, Japan
Mol Biol Cell 12:1367-80. 2001..In contrast, the phenotype of myo5 null cells was indistinguishable from that of wild-type cells. This and other data suggest that Myo5 has a role distinct from that of Myo4...
- Involvement of actin and myosins in Plasmodium berghei ookinete motilityInga Siden-Kiamos
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Vassilika Vouton, P O Box 1385, 71110 Heraklion, Crete, Greece
Mol Biochem Parasitol 150:308-17. 2006..We also studied the expression during ookinete development of genes encoding actin and two small class XIV myosins, PbMyoA, and PbMyoB...
- Drosophila UNC-45 accumulates in embryonic blastoderm and in muscles, and is essential for muscle myosin stabilityChi F Lee
Department of Biology and the Molecular Biology Institute, San Diego State University, San Diego, CA 92182, USA
J Cell Sci 124:699-705. 2011..Overall, our data indicate that dUNC-45 is important for myosin accumulation and muscle function. Furthermore, our results suggest that dUNC-45 acts post-translationally for proper myosin folding and maturation...
- Human Myo19 is a novel myosin that associates with mitochondriaOmar A Quintero
Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Curr Biol 19:2008-13. 2009..Defects in their localization and dynamics lead to human disease. Myosins are actin-based motors that power processes such as muscle contraction, cytokinesis, and organelle transport...
- Novel mutations of MYO15A associated with profound deafness in consanguineous families and moderately severe hearing loss in a patient with Smith-Magenis syndromeN Liburd
Laboratory of Molecular Genetics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, 5 Research Court, Room 2A 015, Rockville, MD 20850, USA
Hum Genet 109:535-41. 2001..In addition, one hemizygous missense mutation of MYO15A was found in one of eight Smith-Magenis syndrome (del(17)p11.2) patients from North America who had moderately severe sensorineural hearing loss...
Research Grants115 found, 100 shown here
- Chymase-Mediated MMP Activation in Ishemia Reperfusion InjuryLOUIS JOSEPH DELL'ITALIA; Fiscal Year: 2013....
- Neurotrophins in the LungY S Prakash; Fiscal Year: 2012..By establishing the role of neurotrophins in airway narrowing with or without inflammation, the proposed studies will the foundation for better understanding of airway diseases, and potential development of new therapeutic targets. ..
- Dynamics of Kinesins, Dyneins, and MyosinsPaul R Selvin; Fiscal Year: 2010DESCRIPTION (provided by applicant): Molecular motors - kinesin, dynein, and myosins - play a crucial role in the maintenances and development of the organization, motility, and cell signaling, of healthy cells...
- Cellular and Molecular Regulation of ThrombopoiesisRamesh A Shivdasani; Fiscal Year: 2012..Characterizing key molecular pathways of platelet formation should lead to better control of circulating platelet counts and to implementation of less toxic treatments for cancer. ..
- Myosin phosphatase autoinhibition in gastrointestinal smooth muscle contractionAvril V Somlyo; Fiscal Year: 2012..The results of the research should translate into novel treatments for targeting these diseases. ..
- EFFECT OF LIPIDS ON VASCULAR GRAFT HEALINGLinda M Graham; Fiscal Year: 2013..For example, a TRPC6 inhibitor could increase endothelial cell migration and promote healing of arterial injuries and vascular grafts for the benefit all patients who require cardiovascular interventions. ..
- Zot, Zonulin, and Pathophysiology of Intestinal Tight JunctionsAlessio Fasano; Fiscal Year: 2013..With our Aim 3 we will investigate the association of HP genotypes (HP1-1, HP1-2, and HP2-2) with serum zonulin expression and clinical severity in human disease states and transgenic murine models. ..
- Impact of AMP-activated kinase on sex differences in hypertrophic cardiomyopathyJohn P Konhilas; Fiscal Year: 2013..Therefore, it is of major clinical significance that the mechanistic link be determined between cardiac disease, sex/gender differences and energetic regulators in the heart. ) ..
- Molecular control of differential peptide transmitter exocytosis.COREY B SMITH; Fiscal Year: 2013..The data obtained will provide a molecular understanding of the key regulators of the acute sympatho-adrenal stress response as well as pathologies resulting from its improper regulation. ..
- Autoimmunity in the Mediation of Infectious MyocarditisJay Reddy; Fiscal Year: 2013..These studies may then create opportunities to derive immunotherapies in the form of altered peptide ligands and tolerogens as antigen-specific treatment modalities, including personalized therapies, for DCM patients.] ..
- Hypoplastic Left Heart Syndrome: Expression of RHD in the Fetus?Pirooz Eghtesady; Fiscal Year: 2013..Tremendous resources are devoted to its medical management. Therefore, understanding why HLHS happens, the goal of the proposed research would have great significance and relevance to public health. ..
- Human Platelet Defects in Transcription Factor RUNX1 HaplodeficiencyANGARA KONETI RAO; Fiscal Year: 2013..They will advance our understanding of the mechanisms in normal and abnormal platelet function. ..
- SMOOTH MUSCLE MYOSIN PHOSPHATASE SUBUNIT ISOFORMSSteven A Fisher; Fiscal Year: 2013..These studies will provide novel insights regarding the role of Tra2b, and novel links between transcriptional and splicing controls, in the generation and modulation of vascular smooth muscle contractile phenotypic diversity. ..
- The Effect Of Deafness Associated Mutations on MyosinVIIA FunctionEva Forgacs; Fiscal Year: 2010..The biochemical, structural and motile properties of the wild type and mutant myosin VIIA proteins will be studied, to delineate the molecular mechanism of the myosin VIIA related deafness, vestibular dysfunction and blindness. ..
- NFATc3 in chronic hypoxic pulmonary hypertensionLaura V Gonzalez Bosc; Fiscal Year: 2012..A better understanding of this mechanisms in PAH will lead to the development of novel therapeutic approaches to prevent and cure this debilitating disease. ..
- Silencing cardiac-specific microRNAs to prevent pathological remodeling in congesMATTHEW SWAN LAWRENCE; Fiscal Year: 2011..Where rodent hearts express more 1MHC, monkey hearts, like human hearts, predominantly express 2M HC. Both myosins co-express a miRNA-208 isoform that we will designate as miR- 208a (coming from 1MHC) and miR-208b (coming from ..
- Cardiomyocyte Zinc and its Regulation of Contraction-Relaxation FunctionBradley M Palmer; Fiscal Year: 2012..This study could therefore provide a basis for therapies for several diseases or conditions that affect a large component of the population. ..
- Mechanisms of Arsenic-Induced Developmental ToxicityLisa Bain; Fiscal Year: 2009....
- Endothelial Lipoxygenase Metabolites and Vascular ToneWILLIAM BRYSON CAMPBELL; Fiscal Year: 2013..These studies will identify two new mechanisms of action for the endothelium-derived relaxing factor 11,12,15-THETA and provide a new therapeutic approach to enhancing endothelial function. ..
- Mechanics and regulation of chromosome dynamics in meiotic prophaseMICHAEL EDWARD DRESSER; Fiscal Year: 2012..results establish a role for the myosin type II gene MYO1 in a subset of the RPMs and suggest that other myosins influence RPMs either directly or by affecting the rate of actin filament treadmilling...
- Regulation of Endothelial ICAM-1 and PMN-Mediated Lung Injury by Actin DynamicsFabeha Fazal; Fiscal Year: 2013....
- Myosin 15:Genetics, Pathology and Therapeutic PotentialSally A Camper; Fiscal Year: 2013..This team will enable us to exploit the animal models fully to understand the mechanisms of inner ear disease and has the potential to identify novel genes essential for normal hearing. ..
- Regulation of Intestinal Epithelial Tight Junction BarrierThomas Y Ma; Fiscal Year: 2011..The purpose of this grant application is to elucidate the pathogenic mechanisms involved in bacterial antigen induced disruption of intestinal barrier (or development of leaky gut). ..
- Understanding the biology of schistosomes in response to praziquantelCharles Cunningham; Fiscal Year: 2013..More explicit knowledge of the binding target of PZQ and the mechanism of action of the drug will help us to devise improved assays for monitoring the emergence of resistance. ..
- Development of an Inhibitor of Toxoplasma gondiiRichard D Wood; Fiscal Year: 2010..gondii, as treatments for toxoplasmosis which affects HIV/AIDS patients. ..
- Keratin Biology and Disease Aspects in Simple EpitheliaBishr Omary; Fiscal Year: 2013..Our ultimate goal is to better understand the function of keratins and their human disease relevance in digestive organs, and to provide fundamental knowledge on how the keratins family regulates normal digestive organ physiology. ..
- Cellular Mechanisms of Lymphatic Muscle ContractilityMICHAEL JOHN DAVIS; Fiscal Year: 2012..These studies will investigate which proteins allow these vessels to contract so that specific therapeutic agents can be developed to correct lymphatic pump dysfunction and drainage of edematous tissues. ..
- EFFECT OF R1R2 OVER-EXPRESSION ON CARDIAC FUNCTIONMichael Regnier; Fiscal Year: 2013..Results from these studies will provide valuable insight on whether sustained enhancement of myofilament contractility (with dATP) has potential for treatment of heart failure in animal models and humans. ..
- Impact of AMP-activated kinase on sex differences in hypertrophic cardiomyopathyJohn P Konhilas; Fiscal Year: 2013..Therefore, it is of major clinical significance that the mechanistic link be determined between cardiac disease, sex/gender differences and energetic regulators in the heart. ) ..
- Regeneration of stereocilia of cochlear hair cellsDavid Z Z He; Fiscal Year: 2010....
- Physiological Regulation of MLCK in Intact ArteriesWithrow Gil Wier; Fiscal Year: 2013..This research will utilize a mouse model of salt-induced hypertension to provide specific new information on the role of MLCK in high blood pressure. ..
- The Role of Myosin in Vesicle TraffickingYashomati M Patel; Fiscal Year: 2010..The proposed research will provide critical information for understanding the molecular mechanism(s) involved in the development of in type 2 diabetes. ..
- Molecular mechanics of mutant cardiac myosinJeffrey R Moore; Fiscal Year: 2013..This study will examine how FHC mutations in one of the proteins of the heart (the myosin regulatory light chain), alters the ability of the heart to generate force and power at the molecular level. ..
- Development of Inhibitors of the Leukemia Fusion Protein CBFbeta-SMMHCRoger A Rajewski; Fiscal Year: 2013..These inhibitors also have the potential to be less toxic than standard chemotherapy, resulting in reduced side effects from treatment. ..
- Rho Kinase and Airway HyperresponsivenessStephanie A Shore; Fiscal Year: 2013..Thus, the ROCK pathway may be an important and still relatively unexplored therapeutic target in asthma. Understanding the role of ROCKs in mouse models of asthma could lead to new strategies for preventing this disease. ..
- Nitrergic Neuro-smooth Muscle Transmission in the GutRaj K Goyal; Fiscal Year: 2013..This proposal is to understand how these disorders are caused and how better treatments for these disorders can be developed. ..
- Molecular Control of Gut Permeability in TraumaMack H Wu; Fiscal Year: 2013..The research work has potential impact on the development of surgical or medical interventions against gut barrier injury in VA patients with trauma or inflammatory diseases. ..
- Contributions of Cardiac Myosin Binding Protein-C to Healthy and Failing HeartsCARL WEI CHAN TONG; Fiscal Year: 2013..Thus, funding this proposal will help to solve a vexing health problem and enable a beginning clinician scientist toward path of independent research. ..
- Visualizing Actomyosin Transients by Data MergingThomas P Burghardt; Fiscal Year: 2013..These analytical tools are next-generation methods addressing transduction and motility in muscle myosin. ..
- Enhancing Energy Expending Adipocytes in White Adipose TissueSTEPHEN ROBERT FARMER; Fiscal Year: 2013....
- Glycosyltransferase Golgi Retention MechanismPi Wan Cheng; Fiscal Year: 2013..The results will expand our fundamental understanding of the mechanism of intracellular trafficking and Golgi retention of glycosyltransferases and could help develop therapeutic interventions for these two lung diseases in veterans. ..
- Specification of actomyosin function in the cellMatthew Lord; Fiscal Year: 2013..Our use of fission yeast (with 1 Tm isoform and 5 myosins total) will overcome this complexity and provide new and novel insights into the specification of actomyosin ..
- GENETICS OF HERPESVIRUS TRANSFORMATIONDavid M Knipe; Fiscal Year: 2013..Each of these important cell biological problems will be investigated in this proposed research. New antiviral targets for inhibition of herpesviral replication may also be elucidated. ..
- Mechanotransduction in the Ciliary MuscleAndrew D Pucker; Fiscal Year: 2013..This K08-supported research will form the basis for a future R01 grant application for independent research aimed at creating targeted interventions for treating and preventing ciliary muscle related myopic growth. ..
- Cellular and Biochemical Studies of Myosin Assembly in DictyosteliumThomas Egelhoff; Fiscal Year: 2009..The studies we propose addressing the full complement of Dictyostelium alpha kinases will also provide a powerful platform for comparisons and insights into possible roles of alpha kinases in mammalian systems. ..
- Myosin ELC, a novel therapeutic target for FHCDanuta Szczesna-Cordary; Fiscal Year: 2013..These studies will help to determine the role(s) of the ELC and the N-terminal cardiac specific ELC extension in the regulation of contractile force in healthy and FHC-diseased hearts. ..
- MICROFILAMENTS IN THE YEAST SACCHAROMYCES CEREVISIAEAnthony P Bretscher; Fiscal Year: 2013..generally mediated by microtubule-based motors, and short range transport and capture by the actin-based motors, myosins. The myosin-V family of molecular motors is evolutionarily highly conserved between animals, plants and fungi, ..
- Functional Consequences of FHC-linked RLC Mutations.Danuta Szczesna-Cordary; Fiscal Year: 2012....
- LISTERIA AND SHIGELLA USE ACTIN TO SPREAD CELL TO CELLFrederick Southwick; Fiscal Year: 2013..These discoveries can be applied to all forms of cell motility, including tumor metastasis, neurite outgrowth, and platelet spreading. ..
- Interatction of Estrogen, Age and Activity on Musculoskeletal Strength in FemalesDawn A Lowe; Fiscal Year: 2013..The research described in this application will determine how estradiol treatment can benefit estrogen-deficient females by improving muscle strength. ..
- Design and Implementation of Genetically Encoded Myosin Based Force SensorsDANIEL PETER KIEHART; Fiscal Year: 2013..Because myosins play key roles in a variety of processes fundamental to human homeostasis and development, we expect these force ..
- Cardiac Myosin Binding Protein-C: Structure and FunctionSakthivel Sadayappan; Fiscal Year: 2013..abstract_text> ..
- Axial Flow Effects in Proximal TubuleTONG WANG WANG; Fiscal Year: 2012..model predictions with flow-induced changes on Na+ and HCO3- absorption in wild-type, and villin, fimbrin, myosins and cilia-deficient mice;2) To study the role of flow-induced actin cytoskeletal reorganization in modulating ..
- Structural Dynamics of Cardiac Muscle ContractionBrett A Colson; Fiscal Year: 2012..Since defects in this protein cause human heart disease, this research is likely to have substantial impact on therapeutic approaches to heart disease. ..
- Ion Channel Function in Auditory &Vestibular Hair CellsJeffrey R Holt; Fiscal Year: 2013..We expect that this project will produce information that will form the basis for development of future strategies to treat deafness and balance problems in some of the ~28 million Americans who suffer from inner ear disorders. ..
- Calcium-dependent Regulation of Smooth Muscle PhenotypeBRIAN WAMHOFF; Fiscal Year: 2010....
- Signal transduction mechanisms to myosin phosphataseJames T Stull; Fiscal Year: 2013..We will test the hypothesis that the Ca2+-independent kinase ZIPK phosphorylates MYPT1 and CPI-17 as an integral component of the Ca2+-sensitization process. ..
- Mechanism of Thin Filament Regulation of Cardiac Actomyosin HydrolysisHoward White; Fiscal Year: 2009..The combined data will be used to determine the mechanism of regulation by cardiac thin filaments and increase our understanding of mechanism of regulation of cardiac contractility. ..
- Quantitative Analysis of Chemotactic Motility Cycle of Ameboid CellsJUAN CARLOS LASHERAS; Fiscal Year: 2013....
- Genetics and Molecular Biology of Striated Muscle MyosinSanford I Bernstein; Fiscal Year: 2013..Overall our project will take advantage of an innovative experimental system to test significant questions regarding basic myosin function and disease. ..
- Structure and mechanism of the dynein motorRonald D Vale; Fiscal Year: 2013....
- HTS ASSAYS FOR INHIBITORS OF PROTEIN INTERACTIONS INCLUDING MLCK1-FKBP8Jerrold R Turner; Fiscal Year: 2012..In addition, the TR-FRET approach will yield robust high throughout screens to identify agents that modulate a wide variety of PPIs in live cells. ..
- Cytoskeletal Pliability within Cells under Stress: RBCs and Marrow-derived SCsDennis E Discher; Fiscal Year: 2012..New therapeutic strategies that could benefit more broadly include exon skipping strategies that are presently being applied to the spectrin family protein dystrophin [Goyenvalle Science 2006]. ..
- Molecular roles in active and passive mechanics in cochlear hair bundlesANTHONY WEI PENG; Fiscal Year: 2013..The technology developed will greatly enhance auditory research and likely have broader mechanics applications in the auditory field and beyond. ..
- Regulation of Cytokinesis and Tumor Formation by RhoAANN LOUISE MILLER; Fiscal Year: 2013..Finally, I will test whether cytokinesis failure induced by other Rho zone regulators, especially those that are up- or down-regulated or mutated in human tumors, promotes tumor formation. ..
- Signaling pathways that mediate mammalian oocyte cortical mechanicsDouglas N Robinson; Fiscal Year: 2013..This project will extend understanding of the female gamete in a completely new direction - bringing insights from cellular mechanics into our view of the oocyte-to-egg and egg-to-embryo transitions. ..
- Regulation of Endothelial Cell Branching Morphogenesis via MCAK-targted ControlKENNETH ALBERT MYERS; Fiscal Year: 2013....
- Biophysical Mechanisms of Drosophila Development.Jennifer A Zallen; Fiscal Year: 2013....
- Chromatin mechanism of molecular motor gene regulationChing Pin Chang; Fiscal Year: 2013....
- Molecular characteristics of the apical recycling systemJAMES RICHARD GOLDENRING; Fiscal Year: 2013....
- Brain Pericyte and Amyloid-beta Peptide InteractionDonghui Zhu; Fiscal Year: 2013..Using in vitro capillary-like structures, we will idenify the rate-limiting step and key-signaling molecules in pericyte adhesion and migration affected by A[unreadable]. The rate-limiting step would be the ideal target for therapies. ..
- Estrogenic Effects on Aged MuscleDawn A Lowe; Fiscal Year: 2013..The applicant will conduct research to determine how estradiol treatment can benefit estrogen-deficient females by improving muscle strength. ..
- Mutations in Smooth Muscle Contractile Proteins: Pathways to Vascular DiseasesDianna M Milewicz; Fiscal Year: 2013..Project 4 will Investigate SMC cellular pathways leading to thoracic aortic disease and SMC proliferation in these same mouse models. ..
- Role of Nucleo-cytoskeleton Interactions in Cell MigrationGregg G Gundersen; Fiscal Year: 2013....
- Mechanism and Prevention of Female Stress Urinary IncontinenceTom F Lue; Fiscal Year: 2013..This grant proposal will study means to stop birth-trauma mediated disruption of collagen and elastic fibers before it takes place and/or enhance repair of these fibers. ..
- PKD Isoforms in HeartSusan F Steinberg; Fiscal Year: 2013..The overarching goal of these studies is to identify novel regulatory controls and cardiac actions of PKDs that can be targeted to prevent or slow the evolution of cardiac hypertrophy and heart failure phenotypes. ..
- Functional Relevance of CFTR Trafficking in Vivo to Intestinal DiseaseNadia A Ameen; Fiscal Year: 2013....
- Phosphorylation of cMyBP-C Modulates Cardiac ArrhythmiasRamzi J Khairallah; Fiscal Year: 2013..For the PI, the investigation nicely supports his long-term plan of conducting interdisciplinary research related to cardiac function, with the prospect of broadening our understanding of the pathogenesis of heart failure. ..
- Functional crosstalk between myosin II &cofilin in regulation of neuronal growthPaul Forscher; Fiscal Year: 2013..These studies are predicted to have significant clinical implications for understanding neurodegenerative disease and nerve regeneration related to brain and/or spinal cord injury. ..
- TNF-alpha Regulation of Intestinal Paracellular TransportThomas Y Ma; Fiscal Year: 2013..abstract_text> ..
- EM STUDIES ON MUSCLEKENNETH ALLEN TAYLOR; Fiscal Year: 2013..abstract_text> ..
- Using micropost arrays to measure traction forces during dendritic cell motilityDaniel A Hammer; Fiscal Year: 2013..abstract_text> ..
- Mechanisms of Cytokinesis in YeastErfei Bi; Fiscal Year: 2013..structure with a "kink" in the middle of its tail, bearing all the major features of non-muscle type-II myosins in animal cells...
- Dynamic limits on contraction of gastrointestinal smooth muscleMARION JOYCE SIEGMAN; Fiscal Year: 2013....
- Nonmuscle myosins in craniofacial morphogenesis and orofacial cleftingJEFFREY OHMANN BUSH; Fiscal Year: 2013..on our preliminary data, combined with recently published human genetics studies, we believe that non-muscle myosins are excellent candidate drivers of craniofacial morphogenesis...
- Inflammation, miRNA and autophagy in diabetesParas Kumar Mishra; Fiscal Year: 2013..At translational level, the proposal will provide concrete evidence that over expression of miR-133 or ablation of MMP9 can ameliorate diabetic cardiomyopathy. ..
- Thick and Thin Filament Mutations that cause Distal ArthrogryposisALICE WARD RACCA; Fiscal Year: 2013....
- COMPUTER SIMULATION THEORY OF GLOBULAR PROTEIN DYNAMICSJeffrey Skolnick; Fiscal Year: 2013..Each Aim is part of a more comprehensive objective to increase our understanding of subcellular processes and biochemical function that will help accelerate drug discovery as well as provide general biochemical insights. ..
- Polarity-Mediated Suppression of TumorigenesisGREGORY VINCENT SCHIMIZZI; Fiscal Year: 2013..These advances will further illustrate how the loss of cell polarity contributes to tumor development and progression. ..
- Myo1c Participates in Podocyte Junction Formation Through Interaction with Neph1Deepak Nihalani; Fiscal Year: 2013....
- Atomic resolution structure and function of the ribosomeJAMIE H CATE; Fiscal Year: 2013..regard, the ribosome is no different from other molecular machines, such as RNA and DNA polymerases, helicases, myosins and kinesins. All of these molecular machines use conformational changes as a basis for function...
- Genetic Modification of Cardiac Motor Proteins in Normal and Failing MyocytesEric J Devaney; Fiscal Year: 2013..RELEVANCE (See instructions): The overall goal of the application is to assess the results of manipulation of a cardiac motor protein both to induce cardiac dysfunction and to treat it as well. (End of Abstract) ..
- Mechanics of lamellipodial stability, turning and self-polarizationAlexander Mogilner; Fiscal Year: 2013..This work will result in advanced understanding of cell motility, and will also produce broadly applicable novel mathematical tools as well as mathematical model components that can be integrated with existing models of cell migration. ..
- Targeted Rho kinase-1 KO Mouse Model for Vascular Smooth Muscle RemodelingDENIZ TOKSOZ-EXLEY; Fiscal Year: 2013..Overall, the Aims will generate and characterize a novel genetic ROCK animal model, and test the hypothesis in vivo that ROCK1 contributes to remodeling and pulmonary hypertensive disease. ..
- CYTOSKELETAL MECHANISMS OF RETINAL DEVELOPMENTDonald F Ready; Fiscal Year: 2013..If the aims of this project are achieved, key properties of core cell functions will be established in this model. ..
- Structural Dynamics of Stretch Activation in MuscleMICHAEL KAY REEDY; Fiscal Year: 2013....
- Allostery in myosins studied at the molecular levelRonald S Rock; Fiscal Year: 2013..In prior studies, we discovered two myosins with new and distinct processive stepping patterns on actin...
- Molecular mechanisms of cell shape change in cytokinesisAmy Shaub Maddox; Fiscal Year: 2013..In addition, because currently used anti-mitotics also have limited success against some tumor types, development of cytokinesis drugs will be a welcome expansion and diversification of our arsenal against cancers. ..
- Role of the Inflammatory Cytokine Oncostatin-M in Promoting AtherosclerosisErrol Wijelath; Fiscal Year: 2013..This proposed work will elucidate the role of an important biological factor, OSM, in the evolution of the atherosclerosis. It will also identify STAT-1 pathway as a therapeutic target for controlling pathological vascular remodeling. ..
- A novel autophagy gene beclin 2 in the prevention of type 2 diabetes and obesityCongcong He; Fiscal Year: 2013....
- Molecular Characterization of Cardiomyopathy Mutations in Human Cardiac MyosinJAMES ANTHONY SPUDICH; Fiscal Year: 2013..Most studies to determine the functional impact of these mutations on myosin have used non-human myosins and most have not used cardiac myosins...