actins

Summary

Summary: Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.

Top Publications

  1. ncbi Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion
    Akira Orimo
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Cell 121:335-48. 2005
  2. pmc Lifeact: a versatile marker to visualize F-actin
    Julia Riedl
    Max Planck Institute of Biochemistry, Independent Junior Research Group Cellular Dynamics and Cell Patterning, Am Klopferspitz 18, 82152 Martinsried, Germany
    Nat Methods 5:605-7. 2008
  3. pmc ADF/cofilin: a functional node in cell biology
    Barbara W Bernstein
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523 1870, USA
    Trends Cell Biol 20:187-95. 2010
  4. ncbi The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors
    A J Ridley
    Institute for Cancer Research, Chester Beatty Laboratories, London, England
    Cell 70:389-99. 1992
  5. ncbi The molecular motor toolbox for intracellular transport
    Ronald D Vale
    Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA
    Cell 112:467-80. 2003
  6. pmc Structural basis of long-term potentiation in single dendritic spines
    Masanori Matsuzaki
    Department of Cell Physiology, National Institute for Physiological Sciences and The Graduate University of Advanced Studies Sokendai, Myodaiji, Okazaki 444 8787, Japan
    Nature 429:761-6. 2004
  7. pmc T cell activation
    Jennifer E Smith-Garvin
    Abramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Annu Rev Immunol 27:591-619. 2009
  8. ncbi Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase
    M Maekawa
    Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto 606 8315, Japan
    Science 285:895-8. 1999
  9. ncbi The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly
    R Rohatgi
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 97:221-31. 1999
  10. pmc A high precision survey of the molecular dynamics of mammalian clathrin-mediated endocytosis
    Marcus J Taylor
    Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom
    PLoS Biol 9:e1000604. 2011

Research Grants

  1. Mapping for Specialized Domains for FCeRI Signaling &Internalization
    Bridget S Wilson; Fiscal Year: 2013
  2. Development of a Nonpsychotropic Cannabinoid Agonist for the Treatment of Sclerod
    Robert B Zurier; Fiscal Year: 2012
  3. Gamma-Synuclein-Mediated Regulation of Norepinephrine Transporter
    Anita Sidhu; Fiscal Year: 2010
  4. ENEIQUE A MESRI; Fiscal Year: 2016
  5. Regulation of B Cell Function by Membrane-Cytoskeletal Remodeling
    Neetu Gupta; Fiscal Year: 2012
  6. Michael Demetriou; Fiscal Year: 2014
  7. Maria Diakonova; Fiscal Year: 2014
  8. Regulation of epithelial cell homeostasis by actin microfilaments
    Seema Khurana; Fiscal Year: 2010
  9. MARK L DELL'ACQUA; Fiscal Year: 2016
  10. Mary K Sheats; Fiscal Year: 2016

Detail Information

Publications469 found, 100 shown here

  1. ncbi Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion
    Akira Orimo
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Cell 121:335-48. 2005
    ..Our findings indicate that fibroblasts within invasive breast carcinomas contribute to tumor promotion in large part through the secretion of SDF-1...
  2. pmc Lifeact: a versatile marker to visualize F-actin
    Julia Riedl
    Max Planck Institute of Biochemistry, Independent Junior Research Group Cellular Dynamics and Cell Patterning, Am Klopferspitz 18, 82152 Martinsried, Germany
    Nat Methods 5:605-7. 2008
    ..Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells...
  3. pmc ADF/cofilin: a functional node in cell biology
    Barbara W Bernstein
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523 1870, USA
    Trends Cell Biol 20:187-95. 2010
    ..We argue that this ability to respond to physiological changes by modulating those same changes makes the ADF/cofilin protein family a homeostatic regulator or 'functional node' in cell biology...
  4. ncbi The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors
    A J Ridley
    Institute for Cancer Research, Chester Beatty Laboratories, London, England
    Cell 70:389-99. 1992
    ..These data imply that rho is essential specifically for the coordinated assembly of focal adhesions and stress fibers induced by growth factors...
  5. ncbi The molecular motor toolbox for intracellular transport
    Ronald D Vale
    Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA
    Cell 112:467-80. 2003
    ..Remarkably, fungi, parasites, plants, and animals have distinct subsets of Toolbox motors in their genomes, suggesting an underlying diversity of strategies for intracellular transport...
  6. pmc Structural basis of long-term potentiation in single dendritic spines
    Masanori Matsuzaki
    Department of Cell Physiology, National Institute for Physiological Sciences and The Graduate University of Advanced Studies Sokendai, Myodaiji, Okazaki 444 8787, Japan
    Nature 429:761-6. 2004
    ..They further suggest that small spines are preferential sites for long-term potentiation induction, whereas large spines might represent physical traces of long-term memory...
  7. pmc T cell activation
    Jennifer E Smith-Garvin
    Abramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Annu Rev Immunol 27:591-619. 2009
    ..complex (MHC) complex pSMAC: peripheral supramolecular activation cluster PTK: protein tyrosine kinase Signal transduction: biochemical events linking surface receptor engagement to cellular responses TCR: T cell antigen receptor..
  8. ncbi Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase
    M Maekawa
    Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto 606 8315, Japan
    Science 285:895-8. 1999
    ..These results indicate that phosphorylation of LIM-kinase by ROCK and consequently increased phosphorylation of cofilin by LIM-kinase contribute to Rho-induced reorganization of the actin cytoskeleton...
  9. ncbi The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly
    R Rohatgi
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 97:221-31. 1999
    ..Therefore, N-WASP and the Arp2/3 complex comprise a core mechanism that directly connects signal transduction pathways to the stimulation of actin polymerization...
  10. pmc A high precision survey of the molecular dynamics of mammalian clathrin-mediated endocytosis
    Marcus J Taylor
    Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom
    PLoS Biol 9:e1000604. 2011
    ....
  11. ncbi Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase)
    K Kimura
    Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma 630 01, Japan
    Science 273:245-8. 1996
    ..Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase...
  12. ncbi Rho GTPases and the control of cell behaviour
    A Hall
    MRC Laboratory for Molecular Cell Biology and Department of Biochemistry and Molecular Biology, Cancer Research UK Oncogene and Signal Transduction Group, University College London, London WC1E 6BT, UK
    Biochem Soc Trans 33:891-5. 2005
    ..We have been analysing the biochemical contributions of Rho GTPases in cell movement and have found that Rac controls cell protrusion, while Cdc42 controls cell polarity...
  13. pmc Actin and alpha-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner
    Colin K Choi
    Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia 22908, USA
    Nat Cell Biol 10:1039-50. 2008
    ..From these studies, we have developed a model for adhesion assembly that clarifies the relative contributions of myosin II and actin polymerization and organization...
  14. ncbi Rho GTPases and the actin cytoskeleton
    A Hall
    Medical Research Council Laboratory for Molecular Cell Biology, Cancer Research Campaign Oncogene and Signal Transduction Group, University College London, Gower Street, London WC1E 6BT, UK
    Science 279:509-14. 1998
    ....
  15. ncbi Actin dynamics control SRF activity by regulation of its coactivator MAL
    Francesc Miralles
    Transcription Laboratory, Room 401, Cancer Research UK London Research Institute, Lincolns Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Cell 113:329-42. 2003
    ..Constitutively cytoplasmic MAL derivatives interfere with MAL redistribution and Rho-actin signaling to SRF. MAL associates with several SRF target promoters regulated via the Rho-actin pathway...
  16. doi Rapid leukocyte migration by integrin-independent flowing and squeezing
    Tim Lämmermann
    Department of Molecular Medicine, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    Nature 453:51-5. 2008
    ..Myosin II-dependent contraction is only required on passage through narrow gaps, where a squeezing contraction of the trailing edge propels the rigid nucleus...
  17. ncbi Proteins of the ADF/cofilin family: essential regulators of actin dynamics
    J R Bamburg
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins 80523, USA
    Annu Rev Cell Dev Biol 15:185-230. 1999
    ..ADF/cofilins are essential for cytokinesis, phagocytosis, fluid phase endocytosis, and other cellular processes dependent upon actin dynamics...
  18. ncbi ARF proteins: roles in membrane traffic and beyond
    CRISLYN D'SOUZA-SCHOREY
    Department of Biological Sciences and the Walther Cancer Institute, University of Notre Dame, Notre Dame, Indiana, USA
    Nat Rev Mol Cell Biol 7:347-58. 2006
    ....
  19. pmc Vinculin controls focal adhesion formation by direct interactions with talin and actin
    Jonathan D Humphries
    Wellcome Trust Centre for Cell Matrix Research, University of Manchester, Manchester M13 9PT, England, UK
    J Cell Biol 179:1043-57. 2007
    ..The vinculin tail, however, is responsible for the functional link of FAs to the actin cytoskeleton. We propose a new model that explains how vinculin orchestrates FAs...
  20. pmc Rho-kinase/ROCK: A key regulator of the cytoskeleton and cell polarity
    Mutsuki Amano
    Department of Cell Pharmacology, Nagoya University, Showa Ku, Japan
    Cytoskeleton (Hoboken) 67:545-54. 2010
    ..This review focuses on the structure, function, and modes of activation and action of Rho-kinase...
  21. pmc Induction of epithelial-mesenchymal transition in alveolar epithelial cells by transforming growth factor-beta1: potential role in idiopathic pulmonary fibrosis
    Brigham C Willis
    Department of Anesthesiology Critical Care Medicine, Childrens Hospital Los Angeles, California, USA
    Am J Pathol 166:1321-32. 2005
    ..These results suggest that AECs undergo EMT when chronically exposed to TGF-beta1, raising the possibility that epithelial cells may serve as a novel source of myofibroblasts in IPF...
  22. ncbi Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
    S A Bustin
    Academic Department of Surgery, St Bartholomew s and the Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, London E1 1BB, UK
    J Mol Endocrinol 25:169-93. 2000
    ..It illustrates the usefulness of these assays by demonstrating the significantly different levels of transcription between individuals of the housekeeping gene family, glyceraldehyde-3-phosphate-dehydrogenase (GAPDH)...
  23. ncbi Myofibroblasts and mechano-regulation of connective tissue remodelling
    James J Tomasek
    Department of Cell Biology, University of Oklahoma Health Sciences Center, P O Box 26901, Oklahoma City, OK 73190, USA
    Nat Rev Mol Cell Biol 3:349-63. 2002
    ....
  24. pmc The 'ins' and 'outs' of podosomes and invadopodia: characteristics, formation and function
    Danielle A Murphy
    Sanford Burnham Medical Research Institute, 10901 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Rev Mol Cell Biol 12:413-26. 2011
    ..Recently, progress has been made in our understanding of the regulatory and functional aspects of podosome and invadopodium biology and their role in human disease...
  25. pmc A nucleator arms race: cellular control of actin assembly
    Kenneth G Campellone
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
    Nat Rev Mol Cell Biol 11:237-51. 2010
    ..Formin nucleators with additional biochemical and cellular activities have also been uncovered. Finally, the Spire, cordon-bleu and leiomodin nucleators have revealed new ways of overcoming the kinetic barriers to actin polymerization...
  26. pmc Focal contacts as mechanosensors: externally applied local mechanical force induces growth of focal contacts by an mDia1-dependent and ROCK-independent mechanism
    D Riveline
    Laboratory of Physical Spectrometry (CNRS, UMR 5588, Joseph Fourier University, French National Center for Scientific Research, BP87, , France
    J Cell Biol 153:1175-86. 2001
    ..Our experiments show that integrin-containing focal complexes behave as individual mechanosensors exhibiting directional assembly in response to local force...
  27. ncbi Nuclear actin regulates dynamic subcellular localization and activity of the SRF cofactor MAL
    Maria K Vartiainen
    Transcription Laboratory, Cancer Research UK, London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Science 316:1749-52. 2007
    ..Fluorescence resonance energy transfer analysis demonstrates that the MAL-actin interaction responds to extracellular signals. Serum-induced signaling is thus communicated to nuclear actin to control a transcriptional regulator...
  28. ncbi Multi-step pericellular proteolysis controls the transition from individual to collective cancer cell invasion
    Katarina Wolf
    Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine and Department of Dermatology, University of Wurzburg, Josef Schneider Strasse 2, 97080 Wurzburg, Germany
    Nat Cell Biol 9:893-904. 2007
    ..Thus, invasive migration and proteolytic ECM remodelling are interdependent processes that control tissue micropatterning and macropatterning and, consequently, individual and collective cell migration...
  29. ncbi The extracellular matrix guides the orientation of the cell division axis
    Manuel Thery
    Biologie du cycle cellulaire et de la motilité, UMR144, CNRS, Institut Curie, 26 rue d Ulm 75248 Paris Cedex 05, France
    Nat Cell Biol 7:947-53. 2005
    ..This segregation is further maintained on the cortex of mitotic cells and used for spindle orientation...
  30. pmc HIV envelope-CXCR4 signaling activates cofilin to overcome cortical actin restriction in resting CD4 T cells
    Alyson Yoder
    Department of Molecular and Microbiology, George Mason University, Manassas, VA 20110, USA
    Cell 134:782-92. 2008
    ..Conversely, induction of active cofilin greatly facilitates it. These findings shed light on viral exploitation of cellular machinery in resting T cells, where chemokine receptor signaling becomes obligatory...
  31. doi Life at the leading edge
    Anne J Ridley
    Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, London SE1 1UL, United Kingdom
    Cell 145:1012-22. 2011
    ..In addition, these different protrusion methods likely act in concert to move cells through complex environments in vivo...
  32. ncbi The small GTP-binding protein rac regulates growth factor-induced membrane ruffling
    A J Ridley
    Institute for Cancer Research, Chester Beatty Laboratories, London, England
    Cell 70:401-10. 1992
    ..We propose that rac and rho are essential components of signal transduction pathways linking growth factors to the organization of polymerized actin...
  33. ncbi Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion
    Tony Yeung
    Institute for Medicine and Engineering, University of Pennsylvania, PA 19104, USA
    Cell Motil Cytoskeleton 60:24-34. 2005
    ..These results support the hypothesis that mechanical factors impact different cell types in fundamentally different ways, and can trigger specific changes similar to those stimulated by soluble ligands...
  34. ncbi A modular design for the clathrin- and actin-mediated endocytosis machinery
    Marko Kaksonen
    Department of Molecular and Cell Biology, University of California, Berkeley, 94720, USA
    Cell 123:305-20. 2005
    ....
  35. pmc Pulsed contractions of an actin-myosin network drive apical constriction
    Adam C Martin
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Nature 457:495-9. 2009
    ..Our results suggest a new model for apical constriction in which a cortical actin-myosin cytoskeleton functions as a developmentally controlled subcellular ratchet to reduce apical area incrementally...
  36. doi Actin dynamics and turnover in cell motility
    Klemens Rottner
    Helmholtz Centre for Infection Research, Inhoffen Strasse 7, 38124 Braunschweig, Germany
    Curr Opin Cell Biol 23:569-78. 2011
    ..Here we will draft the future steps essential for achieving true progress towards the goal to increase our understanding of actin cytoskeleton dynamics driving cell migration...
  37. pmc T cell receptor-proximal signals are sustained in peripheral microclusters and terminated in the central supramolecular activation cluster
    Rajat Varma
    Program in Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine, Department of Pathology, New York University School of Medicine, 540 First Avenue, New York, New York 10016, USA
    Immunity 25:117-27. 2006
    ..Our studies reveal a role for F-actin in TCR signaling beyond microcluster formation...
  38. pmc Nanoscale architecture of integrin-based cell adhesions
    Pakorn Kanchanawong
    National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 468:580-4. 2010
    ..The composite multilaminar protein architecture provides a molecular blueprint for understanding focal adhesion functions...
  39. pmc HIV-1 cell to cell transfer across an Env-induced, actin-dependent synapse
    Clare Jolly
    The Sir William Dunn School of Pathology, University of Oxford, UK
    J Exp Med 199:283-93. 2004
    ..We propose that receptor engagement by Env directs the rapid, actin-dependent recruitment of HIV receptors and adhesion molecules to the interface, resulting in a stable adhesive junction across which HIV infects the target cell...
  40. ncbi A selective block of nuclear actin export stabilizes the giant nuclei of Xenopus oocytes
    Markus T Bohnsack
    Zentrum für Molekulare Biologie der Universität Heidelberg ZMBH, INF 282, D 69120 Heidelberg, Germany
    Nat Cell Biol 8:257-63. 2006
    ..Apparently, their mechanical integrity cannot be maintained by chromatin and the associated nuclear matrix, but instead requires an intranuclear actin-scaffold...
  41. pmc The interaction of IQGAP1 with the exocyst complex is required for tumor cell invasion downstream of Cdc42 and RhoA
    Mika Sakurai-Yageta
    Institut Curie, Centre de Recherche, Paris F 75248, France
    J Cell Biol 181:985-98. 2008
    ..Based on these results, we propose that invadopodia function in tumor cells relies on the coordination of cytoskeletal assembly and exocytosis downstream of Rho guanosine triphosphatases...
  42. pmc A FAM21-containing WASH complex regulates retromer-dependent sorting
    Timothy S Gomez
    Department of Immunology, Division of Oncology Research and Schulze Center for Novel Therapeutics, College of Medicine, Mayo Clinic, Rochester, MN 55905, USA
    Dev Cell 17:699-711. 2009
    ....
  43. pmc Actin, microtubules, and vimentin intermediate filaments cooperate for elongation of invadopodia
    Marie Schoumacher
    Unite Mixte de Recherche 144, Centre National de la Recherche Scientifique, Paris, Cedex 05, France
    J Cell Biol 189:541-56. 2010
    ..These findings provide a link between the epithelial to mesenchymal transition and BM transmigration...
  44. pmc A dichotomy in cortical actin and chemotactic actin activity between human memory and naive T cells contributes to their differential susceptibility to HIV-1 infection
    Weifeng Wang
    National Center for Biodefense and Infectious Diseases, Department of Molecular and Microbiology, George Mason University, Manassas, Virginia 20110, USA
    J Biol Chem 287:35455-69. 2012
    ..These results suggest a key role of chemotactic actin activity in facilitating HIV-1 latent infection of these T cell subsets...
  45. ncbi Spatial regulation of beta-actin translation by Src-dependent phosphorylation of ZBP1
    Stefan Huttelmaier
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Nature 438:512-5. 2005
    ..These sequential events provide both temporal and spatial control over beta-actin mRNA translation, which is important for cell migration and neurite outgrowth...
  46. pmc Actin in dendritic spines: connecting dynamics to function
    Pirta Hotulainen
    Neuroscience Center, University of Helsinki, 00014 Helsinki, Finland
    J Cell Biol 189:619-29. 2010
    ..Therefore, specific mechanisms of actin regulation are integral to the formation, maturation, and plasticity of dendritic spines and to learning and memory...
  47. doi Regulation of actin assembly associated with protrusion and adhesion in cell migration
    Christophe Le Clainche
    Laboratoire d Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, Gif sur Yvette, France
    Physiol Rev 88:489-513. 2008
    ..III) focuses on the function and the regulation of major proteins (vinculin, talin, tensin, and alpha-actinin) that control the nucleation, the binding, and the barbed-end growth of actin filaments in adhesions...
  48. pmc Actin, a central player in cell shape and movement
    Thomas D Pollard
    Department of Molecular, Cellular and Developmental Biology, Yale University, Post Office Box 208103, New Haven, CT 06520 8103, USA
    Science 326:1208-12. 2009
    ..Comparisons of quantitative measurements of reactions in live cells with computer simulations of mathematical models will also help generate meaningful insights...
  49. ncbi Dynamic interactions of cortactin and membrane type 1 matrix metalloproteinase at invadopodia: defining the stages of invadopodia formation and function
    Vira V Artym
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical School, Washington, District of Columbia 20057 1469, USA
    Cancer Res 66:3034-43. 2006
    ..Based on these results, we propose a stepwise model of invadopodia formation and function...
  50. ncbi Differential transmission of actin motion within focal adhesions
    Ke Hu
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Science 315:111-5. 2007
    ....
  51. pmc The trip of the tip: understanding the growth cone machinery
    Laura Anne Lowery
    Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Rev Mol Cell Biol 10:332-43. 2009
    ..The understanding of the functions and regulation of the vehicle and navigator provides new insights into the cell biology of growth cone guidance...
  52. pmc Mechanical integration of actin and adhesion dynamics in cell migration
    Margaret L Gardel
    Institute for Biophysical Dynamics, University of Chicago, Chicago, Illinois 60637, USA
    Annu Rev Cell Dev Biol 26:315-33. 2010
    ....
  53. pmc Linking actin dynamics and gene transcription to drive cellular motile functions
    Eric N Olson
    Department of Molecular Biology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390 9148, USA
    Nat Rev Mol Cell Biol 11:353-65. 2010
    ..This insight stimulated research to better understand the actin-MRTF-SRF circuit and to identify alternative mechanisms that link cytoskeletal dynamics and genome activity...
  54. doi Assembly and biological role of podosomes and invadopodia
    Mario Gimona
    Unit of Actin Cytoskeleton Regulation, Consorzio Mario Negri Sud, Department of Cell Biology and Oncology, Via Nazionale 8a, 66030 Santa Maria Imbaro Chieti, Italy
    Curr Opin Cell Biol 20:235-41. 2008
    ..The stage is now set to unravel their roles in the physiology and pathology of tissue plasticity and repair...
  55. pmc EPLIN mediates linkage of the cadherin catenin complex to F-actin and stabilizes the circumferential actin belt
    Kentaro Abe
    RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Proc Natl Acad Sci U S A 105:13-9. 2008
    ....
  56. pmc Compensation mechanism in tumor cell migration: mesenchymal-amoeboid transition after blocking of pericellular proteolysis
    Katarina Wolf
    Department of Dermatology, University of Wurzburg, 97080 Wurzburg, Germany
    J Cell Biol 160:267-77. 2003
    ....
  57. ncbi Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization
    N Yang
    Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan
    Nature 393:809-12. 1998
    ..Furthermore, insulin and an active form of Rac increase the activity of LIMK-1. Taken together, our results indicate that LIMK-1 participates in Rac-mediated actin cytoskeletal reorganization, probably by phosphorylating cofilin...
  58. doi Actin in action: the interplay between the actin cytoskeleton and synaptic efficacy
    Lorenzo A Cingolani
    MRC Laboratory for Molecular Cell Biology and MRC Cell Biology Unit, University College London, Gower Street, London, WC1E 6BT, UK
    Nat Rev Neurosci 9:344-56. 2008
    ..The aim of this Review is to highlight some of the recent progress in elucidating the role of the actin cytoskeleton in synaptic function...
  59. doi Actin dynamics at the leading edge: from simple machinery to complex networks
    Robert H Insall
    CRUK Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, Scotland
    Dev Cell 17:310-22. 2009
    ..Future progress depends on connecting these processes and components to the dynamic behavior of the whole cell in three dimensions...
  60. pmc Adherens and tight junctions: structure, function and connections to the actin cytoskeleton
    Andrea Hartsock
    Departments of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305 5430, USA
    Biochim Biophys Acta 1778:660-9. 2008
    ....
  61. ncbi Rapid and persistent modulation of actin dynamics regulates postsynaptic reorganization underlying bidirectional plasticity
    Ken ichi Okamoto
    RIKEN MIT Neuroscience Research Center, The Picower Center for Learning and Memory, Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Neurosci 7:1104-12. 2004
    ..This bidirectional regulation of actin is actively involved in protein assembly and disassembly and provides a substrate for bidirectional synaptic plasticity...
  62. doi A two-tiered mechanism for stabilization and immobilization of E-cadherin
    Matthieu Cavey
    Institut de Biologie du Développment de Marseille Luminy, UMR 6216 CNRS Université de la Méditerranée, Campus de Luminy Case 907, 13288 Marseille Cedex 09, France
    Nature 453:751-6. 2008
    ..Uncoupling stability and mobility of E-cadherin complexes suggests that stable epithelia may remodel through the regulated mobility of very stable adhesive foci...
  63. pmc p27Kip1 modulates cell migration through the regulation of RhoA activation
    Arnaud Besson
    Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Division of Basic Science, Seattle, WA 98109, USA
    Genes Dev 18:862-76. 2004
    ..Together, the data suggest a novel role for p27(Kip1) in regulating cell migration via modulation of the Rho pathway...
  64. ncbi Rho GTPases and actin dynamics in membrane protrusions and vesicle trafficking
    Anne J Ridley
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, 91 Riding House Street, London W1W 7BS, UK
    Trends Cell Biol 16:522-9. 2006
    ....
  65. doi The nature of the globular- to fibrous-actin transition
    Toshiro Oda
    X ray Structural Analysis Research Team, RIKEN SPring 8 Center, RIKEN Harima Institute, 1 1 1, Kouto, Sayo, Hyogo 679 5148, Japan
    Nature 457:441-5. 2009
    ..The flat form is essential for the formation of stable, helical F-actin. Our F-actin structure model provides the basis for understanding actin polymerization as well as its molecular interactions with actin-binding proteins...
  66. pmc Direct dynamin-actin interactions regulate the actin cytoskeleton
    Changkyu Gu
    Nephrology Division, Department of Medicine, Harvard Medical School and Massachusetts General Hospital, Charlestown, MA 02129, USA
    EMBO J 29:3593-606. 2010
    ..Together, our results support a model in which assembled dynamin, generated through interactions with short actin filaments, promotes actin polymerization via displacement of actin-CPs...
  67. doi Direct visualization of secondary structures of F-actin by electron cryomicroscopy
    Takashi Fujii
    Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
    Nature 467:724-8. 2010
    ....
  68. ncbi Imaging actin and dynamin recruitment during invagination of single clathrin-coated pits
    Christien J Merrifield
    Vollum Institute L 474, Oregon Health and Sciences University, 3181 SW Sam Jackson Park Rd, Portland, OR 97201, USA
    Nat Cell Biol 4:691-8. 2002
    ..Therefore, dynamin may provide the trigger and actin may provide the force for movement into the cytosol...
  69. pmc Ezrin self-association involves binding of an N-terminal domain to a normally masked C-terminal domain that includes the F-actin binding site
    R Gary
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA
    Mol Biol Cell 6:1061-75. 1995
    ..The conformationally inaccessible C-terminal region included the F-actin binding site, suggesting that this activity is likewise regulated by masking...
  70. ncbi Rho and Rac take center stage
    Keith Burridge
    Department of Cell and Developmental Biology and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Cell 116:167-79. 2004
    ..Additionally, we will discuss how there is signaling crosstalk between family members and how various bacterial pathogens have developed strategies to manipulate Rho protein activity so as to enhance their own survival...
  71. pmc Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence
    Jochen Guck
    Institute for Soft Matter Physics, Department of Physics and Geosciences, University of Leipzig, 04103 Leipzig, Germany
    Biophys J 88:3689-98. 2005
    ..This suggests using optical deformability as an inherent cell marker for basic cell biological investigation and diagnosis of disease...
  72. pmc Cells respond to mechanical stress by rapid disassembly of caveolae
    Bidisha Sinha
    Université P et M Curie CNRS UMR, Paris, France
    Cell 144:402-13. 2011
    ..Our findings support a new role for caveolae as a physiological membrane reservoir that quickly accommodates sudden and acute mechanical stresses...
  73. doi External forces control mitotic spindle positioning
    Jenny Fink
    Institut Curie, CNRS UMR 144, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Nat Cell Biol 13:771-8. 2011
    ..We propose that cells divide according to cues provided by their mechanical micro-environment, aligning daughter cells with the external force field...
  74. ncbi Interaction with IQGAP1 links APC to Rac1, Cdc42, and actin filaments during cell polarization and migration
    Takashi Watanabe
    Department of Cell Pharmacology, Nagoya University, Graduate School of Medicine, 65 Tsurumai, Showa, Nagoya, Aichi 466 8550, Japan
    Dev Cell 7:871-83. 2004
    ....
  75. ncbi Cell surface mechanics and the control of cell shape, tissue patterns and morphogenesis
    Thomas Lecuit
    Institute of Developmental Biology of Marseille Luminy, UMR6216 CNRS Université de la Méditerranée, Campus de Luminy Case 907, 13288 Marseille Cedex 09, France
    Nat Rev Mol Cell Biol 8:633-44. 2007
    ..In this context, a range of developmental phenomena can be explained by the regulation of cell surface tension...
  76. doi Unleashing formins to remodel the actin and microtubule cytoskeletons
    Melissa A Chesarone
    Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, Massachusetts 02454, USA
    Nat Rev Mol Cell Biol 11:62-74. 2010
    ..The emerging complexity in the mechanisms governing formins mirrors the wide range of essential functions that they perform in cell motility, cell division and cell and tissue morphogenesis...
  77. ncbi Serum response factor: master regulator of the actin cytoskeleton and contractile apparatus
    Joseph M Miano
    Cardiovascular Research Institute, University of Rochester School of Medicine, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Am J Physiol Cell Physiol 292:C70-81. 2007
    ..This review summarizes the recent genomic and genetic analyses of CArG-SRF that support its role as an ancient, master regulator of the actin cytoskeleton and contractile machinery...
  78. ncbi The myofibroblast in wound healing and fibrocontractive diseases
    G Gabbiani
    Department of Pathology, CMU University of Geneva, 1 rue Michel Servet, 1211 Geneva 4, Switzerland
    J Pathol 200:500-3. 2003
    ..These factors also play important roles in the development of fibrocontractive changes, such as those observed in liver cirrhosis, renal fibrosis, and stroma reaction to epithelial tumours...
  79. pmc Vinculin potentiates E-cadherin mechanosensing and is recruited to actin-anchored sites within adherens junctions in a myosin II-dependent manner
    Quint le Duc
    Hubrecht Institute, University Medical Centre Utrecht, 3584 CT Utrecht, Netherlands
    J Cell Biol 189:1107-15. 2010
    ..These findings have implications for barrier function, morphogenesis, cell migration, and invasion and may extend to all soft tissues in which classical cadherins regulate cell-cell adhesion...
  80. pmc Linear arrays of nuclear envelope proteins harness retrograde actin flow for nuclear movement
    G W Gant Luxton
    Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, USA
    Science 329:956-9. 2010
    ....
  81. pmc Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton
    Ke Xu
    Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA
    Nat Methods 9:185-8. 2012
    ..We observed two vertically separated layers of actin networks with distinct structural organizations in sheet-like cell protrusions...
  82. doi Roles for actin assembly in endocytosis
    Olivia L Mooren
    Department of Cell Biology and Physiology, Washington University School of Medicine, Saint Louis, Missouri 63110, USA
    Annu Rev Biochem 81:661-86. 2012
    ..We focus mainly on new findings on the relative importance of actin in clathrin-mediated endocytosis (CME) in yeast versus mammalian cells...
  83. ncbi Caspase-3-generated fragment of gelsolin: effector of morphological change in apoptosis
    S Kothakota
    Chiron Corporation, Emeryville, CA 94608, USA
    Science 278:294-8. 1997
    ..Thus, cleaved gelsolin may be one physiological effector of morphologic change during apoptosis...
  84. ncbi RIAM, an Ena/VASP and Profilin ligand, interacts with Rap1-GTP and mediates Rap1-induced adhesion
    Esther M Lafuente
    Department of Medical Oncology, Dana Farber Cancer Institute, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 7:585-95. 2004
    ..RIAM knockdown displaced Rap1-GTP from the plasma membrane and abrogated Rap1-induced adhesion. Thus, RIAM links Rap1 to integrin activation and plays a role in regulating actin dynamics...
  85. pmc The F-actin severing protein cofilin-1 is required for RNA polymerase II transcription elongation
    Ales Obrdlik
    Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden
    Nucleus 2:72-9. 2011
    ..We speculate that cofilin-1 performs its function in pol II transcription by regulating polymerization of gene-associated actin...
  86. doi Nuclear actin and myosins: life without filaments
    Primal de Lanerolle
    Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, Illinois 60612, USA
    Nat Cell Biol 13:1282-8. 2011
    ..Increasing evidence on their functional roles has highlighted the importance of these proteins in the nuclear compartment...
  87. pmc The Arp2/3 activator WASH regulates α5β1-integrin-mediated invasive migration
    Tobias Zech
    Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK
    J Cell Sci 124:3753-9. 2011
    ..Thus, we implicate WASH and Arp2/3-driven actin nucleation in receptor recycling leading to invasive motility...
  88. pmc Multi-isotope imaging mass spectrometry reveals slow protein turnover in hair-cell stereocilia
    Duan Sun Zhang
    Department of Neurobiology, Harvard Medical School and Howard Hughes Medical Institute, Boston, Massachusetts 02115, USA
    Nature 481:520-4. 2012
    ..Thus, rapid turnover in stereocilia occurs only at the tips and not by a treadmilling process...
  89. ncbi Regulated portals of entry into the cell
    Sean D Conner
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Nature 422:37-44. 2003
    ....
  90. pmc Bone marrow-derived progenitor cells in pulmonary fibrosis
    Naozumi Hashimoto
    Department of Pathology, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Clin Invest 113:243-52. 2004
    ..Thus the collagen-producing lung fibroblasts in pulmonary fibrosis can also be derived from BM progenitor cells...
  91. pmc Actin-based motility drives baculovirus transit to the nucleus and cell surface
    Taro Ohkawa
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    J Cell Biol 190:187-95. 2010
    ..Temporal coordination of actin-based nuclear and surface translocation likely enables rapid transmission to neighboring cells during infection in insects and represents a distinctive evolutionary strategy for overcoming host defenses...
  92. doi Nebulin and N-WASP cooperate to cause IGF-1-induced sarcomeric actin filament formation
    Kazunori Takano
    Department of Biology, Graduate School of Science, Chiba University, 1 33 Yayoicho, Inageku, Chiba 263 8522, Japan
    Science 330:1536-40. 2010
    ..These findings present the mechanisms of IGF-1-induced actin filament formation in myofibrillogenesis required for muscle maturation and hypertrophy and a mechanism of actin nucleation...
  93. doi Endocytosis unplugged: multiple ways to enter the cell
    Sudha Kumari
    National Centre for Biological Science TIFR, Bellary Road, Bangalore 560 065, Karnataka, India
    Cell Res 20:256-75. 2010
    ..Here we provide an account of various endocytic routes, their mechanisms of operation and occurrence across phyla...
  94. ncbi Nanotubular highways for intercellular organelle transport
    Amin Rustom
    Interdisciplinary Center of Neuroscience IZN, Institute of Neurobiology, University of Heidelberg, INF 364, Heidelberg 69120, Germany
    Science 303:1007-10. 2004
    ..Accordingly, we propose a novel biological principle of cell-to-cell interaction based on membrane continuity and intercellular transfer of organelles...
  95. ncbi Control of the assembly of ATP- and ADP-actin by formins and profilin
    David R Kovar
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Cell 124:423-35. 2006
    ..These results suggest that diverse formins are mechanistically similar, but the rates of particular assembly steps vary...
  96. doi Pulsed forces timed by a ratchet-like mechanism drive directed tissue movement during dorsal closure
    Jerome Solon
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Cell 137:1331-42. 2009
    ..Our work uncovers a sophisticated mechanism of cooperative force generation between two major forces driving morphogenesis...
  97. doi Reference gene selection for quantitative real-time PCR normalization in tomato subjected to nitrogen, cold, and light stress
    Trond Løvdal
    Faculty of Science and Technology, Centre for Organelle Research, University of Stavanger, N 4036 Stavanger, Norway
    Anal Biochem 387:238-42. 2009
    ..Thus, the results highlight the need for normalizing gene expression in tomato using the geometric average of multiple carefully selected reference genes...
  98. pmc Functional genomic screen for modulators of ciliogenesis and cilium length
    Joon Kim
    Department of Neurosciences, Institute for Genomic Medicine, Howard Hughes Medical Institute, University of California San Diego, La Jolla, California 92093, USA
    Nature 464:1048-51. 2010
    ..Our results uncover a critical regulatory step that couples actin dynamics and endocytic recycling with ciliogenesis, and also provides potential target molecules for future study...
  99. ncbi Shank, a novel family of postsynaptic density proteins that binds to the NMDA receptor/PSD-95/GKAP complex and cortactin
    S Naisbitt
    Howard Hughes Medical Institute, Department of Neurobiology, Massachusetts General Hospital and Harvard Medical School, Boston 02114, USA
    Neuron 23:569-82. 1999
    ..Shank may function as a scaffold protein in the PSD, potentially cross-linking NMDA receptor/PSD-95 complexes and coupling them to regulators of the actin cytoskeleton...
  100. ncbi Roles of the fission yeast formin for3p in cell polarity, actin cable formation and symmetric cell division
    B Feierbach
    Department of Microbiology, Columbia University College of Physicians and Surgeons, 701 W 168th Street, New York, NY 10032, USA
    Curr Biol 11:1656-65. 2001
    ..Formins are proteins with conserved roles in cell polarity, cytokinesis, and the regulation of actin and microtubule cytoskeletons...
  101. ncbi The stroma reaction myofibroblast: a key player in the control of tumor cell behavior
    Alexis Desmouliere
    Groupe de Recherches pour l Etude du Foie, INSERM E0362, Institut Federatif de Recherche 66, Pathologies Infectieuses et Cancers, Universite Victor Segalen Bordeaux 2, 33076 Bordeaux Cedex, France
    Int J Dev Biol 48:509-17. 2004
    ..On this basis we suggest that the myofibroblast may represent a new important target of antitumor therapy...

Research Grants74

  1. Mapping for Specialized Domains for FCeRI Signaling &Internalization
    Bridget S Wilson; Fiscal Year: 2013
    ..Results of these analyses will contribute important new insight into the nature and assembly of membrane microdomains controlling immune cell signaling. ..
  2. Development of a Nonpsychotropic Cannabinoid Agonist for the Treatment of Sclerod
    Robert B Zurier; Fiscal Year: 2012
    ..The proposed experiments will determine in animal models the efficacy and mechanisms of action responsible for the antifibrotic effects of ajulemic acid (AJA). ..
  3. Gamma-Synuclein-Mediated Regulation of Norepinephrine Transporter
    Anita Sidhu; Fiscal Year: 2010
    ..This grant is also relevant for the ARRA in that it will immediately create 3 new professional jobs. ..
  4. ENEIQUE A MESRI; Fiscal Year: 2016
    ..So our work will allow us to not only to identify mechanisms of KS carcinogenesis but also will help to identify potential new target and new drugs for anti-KS therapy. ..
  5. Regulation of B Cell Function by Membrane-Cytoskeletal Remodeling
    Neetu Gupta; Fiscal Year: 2012
    ..Lessons learnt from understanding the role of ezrin in B cell function can be applied towards therapeutic design for B cell-dependent human diseases. ..
  6. Michael Demetriou; Fiscal Year: 2014
    ..Here we further explore the mechanisms by which protein glycosylation regulates immune function and autoimmunity. ..
  7. Maria Diakonova; Fiscal Year: 2014
    ..Understanding how these proteins work together will help to design new therapeutic approaches and possibly drugs for treatment of different human diseases and breast cancer. ..
  8. Regulation of epithelial cell homeostasis by actin microfilaments
    Seema Khurana; Fiscal Year: 2010
    ....
  9. MARK L DELL'ACQUA; Fiscal Year: 2016
    ....
  10. Mary K Sheats; Fiscal Year: 2016
    ....
  11. Christie P Thomas; Fiscal Year: 2014
    ....
  12. Glutamate receptor recruitment to new synapses in vivo
    PHILIP ERIC WASHBOURNE; Fiscal Year: 2013
    ..Our research will help understand the mechanisms by which synapses form and bring us closer to identifying the molecular deficits in individuals with autism and mental retardation. ..
  13. PATHOPHYSIOLOGY OF THE ENDOTHELIUM
    Francis W Luscinskas; Fiscal Year: 2013
    ..g., heart attacks and strokes), as well as other organs and tissues of the body. These mechanistic insights may help identify novel therapeutic targets for the treatment of a broad spectrum of inflammatory diseases. ..
  14. FASEB SUMMER CONFERENCE ON PHOSPHOLIPASES
    Alex Brown; Fiscal Year: 2010
    ..The funds requested in this proposal will make an invaluable contribution to the support of this conference and in particular the development of young investigators. ..
  15. Dynamics of Kinesins, Dyneins, and Myosins
    Paul R Selvin; Fiscal Year: 2010
    ..as a function of insertion length of the cargo-binding domain of myosin VI (with L. Sweeney, UPenn.). d) Technological developments: Optics/microscopy needs to be achieved to fulfill the above goals. ..
  16. Diverse Roles for RIC8 During G Protein Signaling in Fungi
    Katherine A Borkovich; Fiscal Year: 2012
    ....
  17. Christin Carter-Su; Fiscal Year: 2016
    ....
  18. RSK-2 regulates integrin-mediated adhesion and migration
    JOE WILLIAM RAMOS; Fiscal Year: 2013
    ..This will determine if these proteins are suitable targets for the development of new adhesion specific disease therapies. In particular we will test if RSK2 can serve as a potential new therapeutic target in glioma. ..
  19. Osteoclastic Protein-Tyrosine Phosphatase and Resorption
    Kin Hing William Lau; Fiscal Year: 2013
    ..This project may allow disclose novel targets for development of more effective anti-resorptive therapies for osteoporosis and related disease. Thus, this project is highly relevant to the VA patient care mission. ..
  20. Neuropilin and semaphorin, anti-angiogenesis activities and mechanisms
    Michael Klagsbrun; Fiscal Year: 2009
    ..abstract_text> ..
  21. MINORITY PREDOCTORAL FELLOWSHIP PROGRAM
    Quentin J Baca; Fiscal Year: 2010
    ..The applicant is an excellent candidate for this award and has outstanding potential to combine basic science with clinical applications as an MD-PhD. ..
  22. Melinda Sheffield-Moore; Fiscal Year: 2014
    ..Results from this study may lead to better ways of preventing cancer-related weight loss, and improve the quality of life and survival of those affected by cervical cancer. ..
  23. MECHANISM OF DRUG TRANSPORT IN LUNG CANCER CELLS
    Sanjay Awasthi; Fiscal Year: 2011
    ....
  24. GARY ANDREW WEISMAN; Fiscal Year: 2016
    ..abstract_text> ..
  25. Marc B Hershenson; Fiscal Year: 2015
    ..Completion of this work will provide insight into the pathogenesis of BPD, lead to the development of more accurate biomarkers, and provide new information on the role of matricellular proteins in lung fibrosis. ..
  26. Identifying underlying mechanisms of intracellular changes in response to caregiv
    MELANIE SARAH FLINT; Fiscal Year: 2013
    ..The data obtained will be critical in identifying caregivers at risk for negative outcomes to improve overall caregiver health. ..
  27. Fic-mediated Adenylylation
    CAROLYN ANN WORBY; Fiscal Year: 2013
    ..This covalent addition of AMP blocks signal transduction pathways in the host that are important for combating bacterial infections. ..
  28. HANNAH CHASE KINNEY; Fiscal Year: 2015
    ..Our goal is to determine the fundamental basis of this deficiency by state-of-the-art techniques in human brainstem analysis. Such knowledge is essential to the future development of a means to identify and treat living infants at risk. ..
  29. Frances M Brodsky; Fiscal Year: 2014
    ....
  30. Alessio Fasano; Fiscal Year: 2015
    ..With our Aim 3 we will investigate the association of HP genotypes (HP1-1, HP1-2, and HP2-2) with serum zonulin expression and clinical severity in human disease states and transgenic murine models. ..
  31. Identifying Key Structural Elements in the Activation of the Arp2/3 Complex
    Patricia A Khuu; Fiscal Year: 2013
    ..Arp2/3 is also involved in metastasis as it is required in the formation of cellular structures such as lamellipodia of migrating cells and invadopodia of cancers cells [5, 6]. ..
  32. Anthrax Toxins Impair Phagocyte Actin-based Motility
    Frederick Southwick; Fiscal Year: 2010
    ....
  33. Apoptosis and Trophoblast Fusion
    NEAL STEWART ROTE; Fiscal Year: 2010
    ..We will investigate those correlations by specifically inhibiting these proteins using peptide inhibitors and gene silencing. Additional studies will evaluate the role of signal transduction through NFB. ..
  34. Maha Abdellatif; Fiscal Year: 2016
    ..2) Determine the role of TFIIB in gene transcription and the development of cardiac hypertrophy. 3) Determine the role of Cdk9 in gene transcription and the development of cardiac hypertrophy. ..
  35. Adhesion of Leptospira interrogans to Human Cell Surface Receptors
    Jenifer L Coburn; Fiscal Year: 2010
    ..abstract_text> ..
  36. ECM compliance and cell cycle control
    RICHARD ASSOIAN; Fiscal Year: 2012
    ....
  37. JONATHAN NOAH THON; Fiscal Year: 2014
    ..Neither the specific aims nor the research goals listed in this application overlap with those of my mentors, and I have recieved permission to take them with me to my own research lab. ..
  38. Thomas S Monahan; Fiscal Year: 2016
    ..Monahan is fortunate to be part of a larger vascular biology research team, whose support will contribute to his ability to achieve his long-term goal of performing clinically relevant, basic science research. ..
  39. Michael R Freeman; Fiscal Year: 2014
    ..DRF3 loss and the MAT may also be functionally important in other solid tumor systems. Consequently, these studies may provide information of more general significance to human cancer. ..
  40. Serrine S Lau; Fiscal Year: 2014
    ....
  41. TRANSFORMING GROWTH FACTOR - BETA MEDIATES EFFECTS OF HYPOXIA IN NEWBORN LUNG
    Namasivayam Ambalavanan; Fiscal Year: 2012
    ....
  42. Michael K Georgieff; Fiscal Year: 2015
    ....
  43. WI State Public Health Lab Capacity for SCID Screening
    CHARLES DENNIS BROKOPP; Fiscal Year: 2010
    ..Our SCID-NBS program will not only produce essential baseline data (i.e. incidence rates in the target population), it will detect SCID afflicted newborns born in year 01 through 03. ..
  44. Scott J Russo; Fiscal Year: 2016
    ....
  45. Ellen Merrick Schill; Fiscal Year: 2015
    ..These experiments will examine if ibuprofen could be a common and avoidable exposure during pregnancy that increases HSCR risk by reducing the efficiency of ENCC migration. ..
  46. Rho-Modifying Cytotoxic Necrotizing Factor of E. coli
    ALISON DAVIS O'BRIEN; Fiscal Year: 2013
    ..attempt to reduce the extent of inflammation and damage evoked by CP9 through parenteral and/or mucosal immunization of mice with a CNF1/Hly toxoid cocktail. ..
  47. Enzymology of Chlamydia Pathogenesis
    Dewey G McCafferty; Fiscal Year: 2013
    ..abstract_text> ..
  48. INSULIN ACTION IN MUSCLE AND FAT CELLS
    JAMES CARLTON GARRISON; Fiscal Year: 2010
    ..Lentivirus will be used to overexpress rictor or to express shRNA to knockdown rictor in 3T3-L1 adipocytes. To investigate mTORC2 function in vivo, we propose to knockout rictor in adipocytes and skeletal muscle of mice. ..
  49. Targeting Tight Junctions To Treat Mucositis
    FREDERICK GARY TOBACK; Fiscal Year: 2010
    ..This project is aimed at learning how this protein fragment exerts its biological effects because when it is given to mice or hamsters with experimental oral mucositis, it protects against and speeds recovery from injury. ..
  50. SIGNALING THROUGH RHO GTP/GDP EXCHANGE FACTORS
    Philip B Wedegaertner; Fiscal Year: 2013
    ..The research in this application will provide new knowledge about the functions of RhoGEFs and thus better define how the RhoGEFs can be therapeutically inhibited in disease without affecting their critical normal functions. ..
  51. Functional analysis of mDia formins in hematopoietic stem cell engraftment and mi
    Peng Ji; Fiscal Year: 2010
    ..Understanding these processes is highly relevant for bone-marrow transplantation therapies to treat blood-related diseases. ..
  52. Mary C Beckerle; Fiscal Year: 2015
    ....
  53. Warren G Hill; Fiscal Year: 2014
    ..This project will provide new knowledge of the mechanisms by which the bladder senses filling and responds and in so doing may suggest new therapeutic options for bladder disease. ..
  54. Tengis S Pavlov; Fiscal Year: 2014
    ..The K99/R00 grant fits to my career goals providing excellent opportunity to assist in transitioning to a stable independent research position with NIH or other independent research funding. ..
  55. Kenneth R Johnson; Fiscal Year: 2016
    ..Aim 5 of this proposal is to refine the chromosomal locations and test candidate genes for three newly mapped loci that contribute to AHL in A/J mice (ahl9 on Chr 11) and NOD/ShiLtJ mice (ahl10 on Chr 1 and ahl11 on Chr 6). ..
  56. Barry M Willardson; Fiscal Year: 2016
    ..An understanding of the way these protein complexes are brought together is necessary to develop treatments that would allow the complexes to assemble and function despite the mutations. ..
  57. NFATc3 in chronic hypoxic pulmonary hypertension
    Laura V Gonzalez Bosc; Fiscal Year: 2012
    ..A better understanding of this mechanisms in PAH will lead to the development of novel therapeutic approaches to prevent and cure this debilitating disease. ..
  58. The Effect Of Deafness Associated Mutations on MyosinVIIA Function
    Eva Forgacs; Fiscal Year: 2010
    ..The biochemical, structural and motile properties of the wild type and mutant myosin VIIA proteins will be studied, to delineate the molecular mechanism of the myosin VIIA related deafness, vestibular dysfunction and blindness. ..
  59. Gadiparthi N Rao; Fiscal Year: 2016
    ....
  60. Christopher E Turner; Fiscal Year: 2016
    ....
  61. HORMONAL REGULATION OF MESSENGER RNA STABILITY
    Daniel R Schoenberg; Fiscal Year: 2013
    ....
  62. Peter J Mohler; Fiscal Year: 2016
    ..abstract_text> ..
  63. USHERIN: STRUCTURAL AND FUNCTIONAL ANALYSIS
    DOMINIC E COSGROVE; Fiscal Year: 2012
    ..This proposal tests the hypothesis that the functional connection is a process called protein translocation, which may pinpoint the defective process that causes deafness and blindness in Usher patients. ..
  64. Dianne Cox; Fiscal Year: 2014
    ....
  65. MOLECULAR MECHANISMS IN NEPHROTIC SYNDROME
    William Smoyer; Fiscal Year: 2001
    ....
  66. Molecular Function of Palladin's Ig Domains in Cell Adhesion and Motility
    Carol A Otey; Fiscal Year: 2010
    ..Thus, understanding the precise molecular function of palladin is likely to yield insights into the cellular mechanisms that control both essential functions in normal cells, and dysfunctions that result in pathological cell behavior. ..
  67. PROFILIN I--AN ESSENTIAL PROTEIN IN CELLS AND ANIMALS
    Pascal Goldschmidt Clermont; Fiscal Year: 1999
    ....
  68. Structural Comparison of Strongly-Bound Actomyosin States
    Dorit Hanein; Fiscal Year: 2009
    ..using state-of-the-art EM image reconstruction approaches and use an array of specifically selected myosins and actins to provide (a) high-resolution structure of F-actin, (b) a detailed, residue-level actomyosin interface ..
  69. ACTIN BASED MOTILITY BY RICKETTSIA RICKETTSII
    ROBERT HEINZEN; Fiscal Year: 2002
    ..Not only will these studies lead to a greater understanding of molecular mechanisms of rickettsial pathogenesis, but they will also provide insight into the dynamic control of actin polymerization. ..
  70. CAPPING ACTIN GROWTH IN ERYTHROID NONERYTHROID CELLS
    Velia Fowler; Fiscal Year: 2004
    ..hypothesis that actin filament ends are dynamic in the erythrocyte membrane skeleton, rhodamine or biotin labeled actins will be introduced into resealed ghosts and subunit exchange or polymerization evaluated by light and electron ..
  71. CRYSTALLOGRAPHY OF ACTIN AND RELATED PROTEINS
    CLARENCE SCHUTT; Fiscal Year: 2001
    ..Profilin from plant pollen is a potent allergen. Structures of plant profilin complexed with endogenous flavonols could lead to improved molecular level understanding of the IgE response in allergic individuals. ..
  72. NEURONAL POLARITY
    KENNETH KOSIK; Fiscal Year: 2000
    ..Finally, they will study the effects of other actin associated proteins on MAP organizational properties in ABP expressing; melanoma cells and in gelsolin deficient neuronal cultures. ..
  73. Protein interactions underlying actin-based motility
    David Sept; Fiscal Year: 2006
    ..This work will give perspective, not only into dendritic nucleation, but the motility of bacteria such as Listeria, and the function of the cell as a whole. ..