intermediate filament proteins

Summary

Summary: Filaments 7-11 nm in diameter found in the cytoplasm of all cells. Many specific proteins belong to this group, e.g., desmin, vimentin, prekeratin, decamin, skeletin, neurofilin, neurofilament protein, and glial fibrillary acid protein.

Top Publications

  1. ncbi The cornified envelope: a model of cell death in the skin
    Eleonora Candi
    Biochemistry Laboratory, IDI IRCCS, c o Department of Experimental Medicine, University of Rome Tor Vergata, 00133 Rome, Italy
    Nat Rev Mol Cell Biol 6:328-40. 2005
  2. ncbi Common loss-of-function variants of the epidermal barrier protein filaggrin are a major predisposing factor for atopic dermatitis
    Colin N A Palmer
    Population Pharmacogenetics Group, Biomedical Research Centre, University of Dundee, Ninewells Hospital and Medical School, Dundee DD1 9SY, UK
    Nat Genet 38:441-6. 2006
  3. ncbi Neural stem and progenitor cells in nestin-GFP transgenic mice
    John L Mignone
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Comp Neurol 469:311-24. 2004
  4. pmc Lessons from loricrin-deficient mice: compensatory mechanisms maintaining skin barrier function in the absence of a major cornified envelope protein
    P J Koch
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Cell Biol 151:389-400. 2000
  5. ncbi Turning blood into brain: cells bearing neuronal antigens generated in vivo from bone marrow
    E Mezey
    Basic Neuroscience Program, Laboratory of Developmental Neurogenetics, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    Science 290:1779-82. 2000
  6. ncbi Filaggrin mutations, atopic eczema, hay fever, and asthma in children
    Stephan Weidinger
    Department of Dermatology and Allergy, Technical University Munich, Munich, Germany
    J Allergy Clin Immunol 121:1203-1209.e1. 2008
  7. ncbi Nestin expression--a property of multi-lineage progenitor cells?
    C Wiese
    In Vitro Differentiation Group, Institute of Plant Genetics and Crop Plant Research IPK Gatersleben, Corrensstrasse 3, 06466, Gatersleben, Germany
    Cell Mol Life Sci 61:2510-22. 2004
  8. ncbi Stratum corneum lipids, skin barrier function and filaggrin mutations in patients with atopic eczema
    J M Jungersted
    Department of Dermatology, Roskilde Hospital, University of Copenhagen, Roskilde, Denmark
    Allergy 65:911-8. 2010
  9. pmc Nestin expression in hair follicle sheath progenitor cells
    Lingna Li
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Proc Natl Acad Sci U S A 100:9958-61. 2003
  10. pmc Epidermal barrier dysfunction and cutaneous sensitization in atopic diseases
    Akiharu Kubo
    Department of Dermatology, Keio University School of Medicine, Shinanomachi 35, Shinjuku, Tokyo 160 8582, Japan
    J Clin Invest 122:440-7. 2012

Detail Information

Publications296 found, 100 shown here

  1. ncbi The cornified envelope: a model of cell death in the skin
    Eleonora Candi
    Biochemistry Laboratory, IDI IRCCS, c o Department of Experimental Medicine, University of Rome Tor Vergata, 00133 Rome, Italy
    Nat Rev Mol Cell Biol 6:328-40. 2005
    ....
  2. ncbi Common loss-of-function variants of the epidermal barrier protein filaggrin are a major predisposing factor for atopic dermatitis
    Colin N A Palmer
    Population Pharmacogenetics Group, Biomedical Research Centre, University of Dundee, Ninewells Hospital and Medical School, Dundee DD1 9SY, UK
    Nat Genet 38:441-6. 2006
    ..These variants also show highly significant association with asthma occurring in the context of atopic dermatitis. This work establishes a key role for impaired skin barrier function in the development of atopic disease...
  3. ncbi Neural stem and progenitor cells in nestin-GFP transgenic mice
    John L Mignone
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Comp Neurol 469:311-24. 2004
    ....
  4. pmc Lessons from loricrin-deficient mice: compensatory mechanisms maintaining skin barrier function in the absence of a major cornified envelope protein
    P J Koch
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Cell Biol 151:389-400. 2000
    ....
  5. ncbi Turning blood into brain: cells bearing neuronal antigens generated in vivo from bone marrow
    E Mezey
    Basic Neuroscience Program, Laboratory of Developmental Neurogenetics, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    Science 290:1779-82. 2000
    ..These findings raise the possibility that bone marrow-derived cells may provide an alternative source of neurons in patients with neurodegenerative diseases or central nervous system injury...
  6. ncbi Filaggrin mutations, atopic eczema, hay fever, and asthma in children
    Stephan Weidinger
    Department of Dermatology and Allergy, Technical University Munich, Munich, Germany
    J Allergy Clin Immunol 121:1203-1209.e1. 2008
    ..However, their role in the development of other atopic diseases such as asthma and rhinitis has not yet been clarified in large population-based studies...
  7. ncbi Nestin expression--a property of multi-lineage progenitor cells?
    C Wiese
    In Vitro Differentiation Group, Institute of Plant Genetics and Crop Plant Research IPK Gatersleben, Corrensstrasse 3, 06466, Gatersleben, Germany
    Cell Mol Life Sci 61:2510-22. 2004
    ....
  8. ncbi Stratum corneum lipids, skin barrier function and filaggrin mutations in patients with atopic eczema
    J M Jungersted
    Department of Dermatology, Roskilde Hospital, University of Copenhagen, Roskilde, Denmark
    Allergy 65:911-8. 2010
    ..Until now, no studies have analysed stratum corneum (SC) lipids combined with skin barrier parameters in subjects of known FLG genotype...
  9. pmc Nestin expression in hair follicle sheath progenitor cells
    Lingna Li
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Proc Natl Acad Sci U S A 100:9958-61. 2003
    ..The expression of the unique protein, nestin, in both neural stem cells and hair follicle stem cells suggests their possible relation...
  10. pmc Epidermal barrier dysfunction and cutaneous sensitization in atopic diseases
    Akiharu Kubo
    Department of Dermatology, Keio University School of Medicine, Shinanomachi 35, Shinjuku, Tokyo 160 8582, Japan
    J Clin Invest 122:440-7. 2012
    ..Clarification of the molecular events underpinning epidermal barrier function and dysfunction should lead to a better understanding of the pathophysiological mechanisms of atopic diseases...
  11. ncbi CNS stem cells express a new class of intermediate filament protein
    U Lendahl
    Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge 02142
    Cell 60:585-95. 1990
    ..These observations extend a model in which transitions in intermediate filament gene expression reflect major steps in the pathway of neural differentiation...
  12. pmc A homozygous frameshift mutation in the mouse Flg gene facilitates enhanced percutaneous allergen priming
    Padraic G Fallon
    Institute of Molecular Medicine, Trinity College Dublin, Ireland
    Nat Genet 41:602-8. 2009
    ....
  13. ncbi Isolation and characterization of multipotent skin-derived precursors from human skin
    Jean G Toma
    Department of Developmental Biology, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada M5G 1X8
    Stem Cells 23:727-37. 2005
    ..Together, these data indicate that SKPs are an endogenous multipotent precursor cell present in human skin that can be isolated and expanded and differentiate into both neural and mesodermal cell types...
  14. pmc Filaggrin in the frontline: role in skin barrier function and disease
    Aileen Sandilands
    Epithelial Genetics Group, Division of Molecular Medicine, Colleges of Life Sciences and Medicine, Dentistry and Nursing, University of Dundee, Dundee DD1 5EH, UK
    J Cell Sci 122:1285-94. 2009
    ..Filaggrin is therefore in the frontline of defence, and protects the body from the entry of foreign environmental substances that can otherwise trigger aberrant immune responses...
  15. ncbi Intermediate filaments: structure, dynamics, function, and disease
    E Fuchs
    Howard Hughes Medical Institute, University of Chicago, Illinois 60637
    Annu Rev Biochem 63:345-82. 1994
  16. pmc Postnatal NG2 proteoglycan-expressing progenitor cells are intrinsically multipotent and generate functional neurons
    Shibeshih Belachew
    Center for Neuroscience Research, Children s Research Institute, Children s National Medical Center, Washington, DC 20010 2970, USA
    J Cell Biol 161:169-86. 2003
    ..These data show that at least a subpopulation of postnatal NG2-expressing cells are CNS multipotent precursors that may underlie adult hippocampal neurogenesis...
  17. ncbi Comprehensive analysis of the gene encoding filaggrin uncovers prevalent and rare mutations in ichthyosis vulgaris and atopic eczema
    Aileen Sandilands
    Epithelial Genetics Group, Human Genetics Unit, Division of Pathology and Neuroscience, University of Dundee, Ninewells Hospital and Medical School, Dundee DD1 9SY, UK
    Nat Genet 39:650-4. 2007
    ..i. = 20-1,136)). We found three additional rare null mutations in this case series, suggesting that the genetic architecture of filaggrin-related atopic dermatitis consists of both prevalent and rare risk alleles...
  18. ncbi Plectin and IFAP-300K are homologous proteins binding to microtubule-associated proteins 1 and 2 and to the 240-kilodalton subunit of spectrin
    H Herrmann
    J Biol Chem 262:1320-5. 1987
    ..Similar characteristics were revealed for corresponding 300-kDa components of various other cell lines, supporting the concept that plectin is a general cytoskeletal cross-linking element, probably of multiple function...
  19. pmc The C terminus of peripherin/rds participates in rod outer segment targeting and alignment of disk incisures
    Beatrice M Tam
    Department of Neuroscience, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    Mol Biol Cell 15:2027-37. 2004
    ..This work describes novel roles for the C terminus of peripherin/rds in targeting and maintaining ROS structure and its potential involvement in inherited retinal degenerations...
  20. ncbi Hornerin is a component of the epidermal cornified cell envelopes
    Julie Henry
    Centre National de Recherche Scientifique CNRS Toulouse III University UMR5165, InstitutFédératif de Recherche 150 INSERM CNRS Université Paul Sabatier Centre HospitalierUniversitaire, Toulouse, France
    FASEB J 25:1567-76. 2011
    ..These data demonstrate that HRNR is a component of cornified cell envelopes of human epidermis. Its reduced expression in AD may contribute to the epidermal barrier defect observed in the disease...
  21. ncbi Loss-of-function variations within the filaggrin gene predispose for atopic dermatitis with allergic sensitizations
    Stephan Weidinger
    Department of Dermatology and Allergy Biederstein, Technical University Munich, Biedersteiner Strasse 29, 80802 Munich, Germany
    J Allergy Clin Immunol 118:214-9. 2006
    ..One of the characteristic features of AD and causative factor for the disease is an impaired epidermal skin barrier based on a primary defect of epidermal differentiation...
  22. ncbi Meta-analysis of filaggrin polymorphisms in eczema and asthma: robust risk factors in atopic disease
    Elke Rodriguez
    Division of Environmental Dermatology and Allergy, Helmholtz Zentrum Munich and ZAUM Center for Allergy and Environment, Technische Universitat Munchen, Munich, Germany
    J Allergy Clin Immunol 123:1361-70.e7. 2009
    ..However, published studies demonstrate differences concerning design and effect size, and conflicting results for asthma have been reported...
  23. pmc Intermediate filaments: primary determinants of cell architecture and plasticity
    Harald Herrmann
    Group Functional Architecture of the Cell, German Cancer Research Center, Heidelberg, Germany
    J Clin Invest 119:1772-83. 2009
    ..Here, we highlight the basic structural and functional properties of IFs and derive a concept of how mutations may affect cellular architecture and thereby tissue construction and physiology...
  24. ncbi The burden of disease associated with filaggrin mutations: a population-based, longitudinal birth cohort study
    John Henderson
    Department of Community Based Medicine, University of Bristol, Bristol, United Kingdom
    J Allergy Clin Immunol 121:872-7.e9. 2008
    ..Atopic disease is a major health problem. Mutations in the filaggrin gene (FLG) confer major susceptibility to eczema and related asthma...
  25. ncbi Intermediate filament protein partnership in astrocytes
    C Eliasson
    Department of Medical Biochemistry, University of Gothenburg, SE 405 30 Gothenburg, Sweden
    J Biol Chem 274:23996-4006. 1999
    ..The function of these structures and the requirement for different types of intermediate filament proteins by individual cells are only partly understood...
  26. pmc Apoptotic photoreceptor cell death in mouse models of retinitis pigmentosa
    C Portera-Cailliau
    Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD 21287 9257
    Proc Natl Acad Sci U S A 91:974-8. 1994
    ..These findings raise the possibility that retinal degenerations may be slowed by interfering with the apoptotic mechanism itself...
  27. ncbi CNS microvascular pericytes exhibit multipotential stem cell activity
    Paula Dore-Duffy
    Department of Neurology, Division of Neuroimmunology, Multiple Sclerosis Clinical Research Center, Wayne State University School of Medicine, Detroit Medical Center, Detroit, Michigan 48201, USA
    J Cereb Blood Flow Metab 26:613-24. 2006
    ..With prolonged culture fully differentiated cells of neural lineage were present. Results indicate that adult CNS microvascular pericytes have neural stem cell capability...
  28. ncbi Intermediate filaments: molecular structure, assembly mechanism, and integration into functionally distinct intracellular Scaffolds
    Harald Herrmann
    Department of Cell Biology, German Cancer Research Center, D 69120 Heidelberg, Germany
    Annu Rev Biochem 73:749-89. 2004
    ..Recent structural work has now begun to shed some light onto the complex fine tuning of structure and function in these fibrous, coiled coil forming multidomain proteins and their contribution to cellular physiology and gene regulation...
  29. ncbi Knockdown of filaggrin impairs diffusion barrier function and increases UV sensitivity in a human skin model
    Michael Mildner
    Department of Dermatology, Medical University of Vienna, Vienna, Austria
    J Invest Dermatol 130:2286-94. 2010
    ..In addition, our results challenge the role of filaggrin in intermediate filament aggregation and establish a link between filaggrin and endogenous UVB protection...
  30. pmc Introducing intermediate filaments: from discovery to disease
    John E Eriksson
    Department of Biology, Abo Akademi University, Turku, Finland
    J Clin Invest 119:1763-71. 2009
    ..e., maintenance of structural and mechanical integrity of cells and tissues. However, many disease conditions seem to link to the nonmechanical functions of IFs, many of which have been defined only in the past few years...
  31. pmc Rapid, widespread, and longlasting induction of nestin contributes to the generation of glial scar tissue after CNS injury
    J Frisen
    Department of Neuroscience, Karolinska Institute, Stockholm, Sweden
    J Cell Biol 131:453-64. 1995
    ....
  32. pmc Filaggrin gene defects and risk of developing allergic sensitisation and allergic disorders: systematic review and meta-analysis
    Rosanne A H M van den Oord
    Allergy and Respiratory Research Group, Centre for Population Health Sciences, University of Edinburgh, Edinburgh EH8 9DX
    BMJ 339:b2433. 2009
    ..To investigate whether filaggrin gene defects, present in up to one in 10 western Europeans and North Americans, increase the risk of developing allergic sensitisation and allergic disorders...
  33. pmc Cytokine modulation of atopic dermatitis filaggrin skin expression
    Michael D Howell
    Department of Pediatrics, National Jewish Medical and Research Center, Denver, CO 80206, USA
    J Allergy Clin Immunol 120:150-5. 2007
    ..Atopic dermatitis (AD) is a chronic inflammatory skin disease that is characterized by a defective skin barrier function. Recent studies have reported mutations of the skin barrier gene encoding filaggrin in a subset of patients with AD...
  34. pmc Disruption of muscle architecture and myocardial degeneration in mice lacking desmin
    D J Milner
    Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Cell Biol 134:1255-70. 1996
    ..The present data demonstrate the essential role of desmin in the maintenance of myofibril, myofiber, and whole muscle tissue structural and functional integrity, and show that the absence of desmin leads to muscle degeneration...
  35. ncbi Neurogenesis in the ependymal layer of the adult rat 3rd ventricle
    Yi Xu
    Department of Anatomy and Neurobiology, Kyoto University Graduate School of Medicine, Yoshidakonoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Exp Neurol 192:251-64. 2005
    ..These results indicate that neural progenitor cells exist in the ependymal layer of the adult rat 3rd ventricle and that they may differentiate into neurons functioning in the hypothalamus...
  36. ncbi Filaggrin mutations in the onset of eczema, sensitization, asthma, hay fever and the interaction with cat exposure
    M L A Schuttelaar
    Departments of Dermatology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
    Allergy 64:1758-65. 2009
    ..Filaggrin gene (FLG) mutations contribute to the development of eczema and asthma, but their contribution to sensitization and hay fever remains unclear...
  37. ncbi Analysis of Taiwanese ichthyosis vulgaris families further demonstrates differences in FLG mutations between European and Asian populations
    C K Hsu
    Department of Dermatology, Hokkaido University Graduate School of Medicine, North 15 West 7, Kita ku, Sapporo 060 8638, Japan
    Br J Dermatol 161:448-51. 2009
    ..Mutations in the gene encoding filaggrin (FLG) were identified to underlie ichthyosis vulgaris (IV) and also shown to predispose to atopic eczema. Until now, no FLG mutations have been described in the Taiwanese population...
  38. ncbi Increased expression of stem cell markers in malignant melanoma
    Walter M Klein
    Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA
    Mod Pathol 20:102-7. 2007
    ..Further studies and characterization of these pathways may also reveal new prognostic markers for a disease whose prognosis in advanced stages is dismal...
  39. ncbi Plasticity of cultured mesenchymal stem cells: switch from nestin-positive to excitable neuron-like phenotype
    Sabine Wislet-Gendebien
    Centre for Cellular and Molecular Neurobiology, University of Liege, 17 Place Delcour, B 4020 Liege, Belgium
    Stem Cells 23:392-402. 2005
    ..We conclude that nestin-positive MSCs can differentiate in vitro into excitable neuron-like cells...
  40. ncbi Clinical severity correlates with impaired barrier in filaggrin-related eczema
    Ikue Nemoto-Hasebe
    Department of Dermatology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    J Invest Dermatol 129:682-9. 2009
    ..64, P<0.05) in filaggrin-related AD, but not in non-filaggrin AD. All these data suggest that experimentally demonstrable skin barrier defects due to FLG mutations may play a crucial role in the pathogenesis of AD...
  41. pmc Abnormal skin barrier in the etiopathogenesis of atopic dermatitis
    Peter M Elias
    Veterans Affairs Medical Center, Department of Dermatology, University of California, San Francisco, 94121, USA
    Curr Opin Allergy Clin Immunol 9:437-46. 2009
    ..We will review here emerging evidence that the inflammation in atopic dermatitis results from inherited and acquired insults to the barrier and the therapeutic implications of this new paradigm...
  42. ncbi Regulation of neural markers nestin and GFAP expression by cultivated bone marrow stromal cells
    S Wislet-Gendebien
    Center for Cellular and Molecular Neurobiology, University of Liege, 17 Place Delcour, B 4020 Liege, Belgium
    J Cell Sci 116:3295-302. 2003
    ....
  43. ncbi TNF-α downregulates filaggrin and loricrin through c-Jun N-terminal kinase: role for TNF-α antagonists to improve skin barrier
    Byung Eui Kim
    Department of Pediatrics, National Jewish Health, Denver, Colorado 80206, USA
    J Invest Dermatol 131:1272-9. 2011
    ..Our current study suggests that TNF inhibits barrier protein expression, and TNF-α antagonists may contribute to clinical improvement in patients with psoriasis by improving barrier protein expression...
  44. ncbi Nestin structure and predicted function in cellular cytoskeletal organisation
    K Michalczyk
    School of Biomedical and Sports Science, Edith Cowan University, Joondalup, Western Australia, Australia
    Histol Histopathol 20:665-71. 2005
    ..Upon differentiation, nestin becomes downregulated and is replaced by tissue-specific intermediate filament proteins. Interestingly, nestin expression is reinduced in the adult during pathological situations, such as ..
  45. pmc Filaggrin genotype in ichthyosis vulgaris predicts abnormalities in epidermal structure and function
    Robert Gruber
    Department of Dermatology, Innsbruck Medical University, Innsbruck, Austria
    Am J Pathol 178:2252-63. 2011
    ..Thus, FLG deficiency provokes alterations in keratinocyte architecture that influence epidermal functions localizing to the extracellular matrix. These results clarify how FLG mutations impair epidermal permeability barrier function...
  46. ncbi Wide spectrum of filaggrin-null mutations in atopic dermatitis highlights differences between Singaporean Chinese and European populations
    H Chen
    Institute of Medical Biology, Immunos, 8A Biomedical Grove, Singapore 138648, Singapore
    Br J Dermatol 165:106-14. 2011
    ..Cohort studies in Europe and Japan have reported an FLG mutation carrier frequency of between 14% and 56%, but the prevalent European FLG mutations are rare or absent in Chinese patients with IV and AD...
  47. ncbi A novel cell-cell junction system: the cortex adhaerens mosaic of lens fiber cells
    Beate K Straub
    Division of Cell Biology, German Cancer Research Center, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
    J Cell Sci 116:4985-95. 2003
    ..We here discuss its possible functional importance for the maintenance of lens structure and functions, notably clear and sharp vision...
  48. pmc Isolation of retinal progenitor and stem cells from the porcine eye
    Ping Gu
    Centre for Vision Sciences, Queens University Belfast, United Kingdom
    Mol Vis 13:1045-57. 2007
    ..However, little is known about porcine RPCs and RSCs. We aimed to identify and characterize in vitro RPCs and RSCs from porcine ocular tissues...
  49. pmc A role for intermediate filaments in determining and maintaining the shape of nerve cells
    Brian T Helfand
    Department of Cell and Molecular Biology, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611, USA
    Mol Biol Cell 14:5069-81. 2003
    ..Furthermore, the results of these experiments demonstrate that peripherin IF are critical determinants of the overall shape and architecture of neurons...
  50. pmc Prevalence of mutations causing retinitis pigmentosa and other inherited retinopathies
    M M Sohocki
    Human Genetics Center, School of Public Health, University of Texas Houston Health Science Center, Houston, Texas, USA
    Hum Mutat 17:42-51. 2001
    ..These data are useful in estimating the frequency of specific mutations and in selecting individuals and families for mutation-specific studies...
  51. ncbi Null mutations in the filaggrin gene (FLG) determine major susceptibility to early-onset atopic dermatitis that persists into adulthood
    Jonathan N W N Barker
    St John s Institute of Dermatology, King s College London, St Thomas s Hospital, London, UK
    J Invest Dermatol 127:564-7. 2007
    ..This study helps to further define the nature of the AD phenotype associated with FLG null alleles...
  52. pmc cDNA sequencing of nuclear lamins A and C reveals primary and secondary structural homology to intermediate filament proteins
    D Z Fisher
    Proc Natl Acad Sci U S A 83:6450-4. 1986
    ..striking homology to a corresponding alpha-helical rod domain that is the structural hallmark of all intermediate filament proteins. However, the lamin alpha-helical domain is 14% larger than that of the intermediate filament proteins...
  53. pmc A neurotoxic peripherin splice variant in a mouse model of ALS
    Janice Robertson
    Centre for Research in Neurosciences, Research Institute of the McGill University Health Centre, McGill University, Montreal, Quebec, H3G 1A4, Canada
    J Cell Biol 160:939-49. 2003
    ..This evidence suggests that expression of neurotoxic splice variants of peripherin may contribute to the neurodegenerative mechanism in ALS...
  54. ncbi Neurogenic subventricular zone stem/progenitor cells are Notch1-dependent in their active but not quiescent state
    Onur Basak
    Department of Molecular Embryology, Max Planck Institute of Immunobiology and Epigenetics, D 79108 Freiburg, Germany
    J Neurosci 32:5654-66. 2012
    ..Therefore, we confirm the importance of Notch signaling for maintaining NSCs and neurogenesis in the adult SVZ and reveal that NSCs display a selective reliance on Notch1 that may be dictated by mitotic state...
  55. pmc Filaggrin loss-of-function mutations are associated with enhanced expression of IL-1 cytokines in the stratum corneum of patients with atopic dermatitis and in a murine model of filaggrin deficiency
    Sanja Kezic
    Coronel Institute of Occupational Health, Academic Medical Center, Amsterdam, The Netherlands
    J Allergy Clin Immunol 129:1031-9.e1. 2012
    ....
  56. ncbi Altered stratum corneum barrier and enhanced percutaneous immune responses in filaggrin-null mice
    Hiroshi Kawasaki
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Allergy Clin Immunol 129:1538-46.e6. 2012
    ..Although various reports suggest a critical role for filaggrin in stratum corneum (SC) barrier formation, the lack of filaggrin-null (Flg(-/-)) mice has hampered detailed in vivo analysis of filaggrin's functions...
  57. ncbi The pathological characteristics of glioma stem cell niches
    Hu He
    Department of Neurosurgery, Anhui Provincial Hospital Affiliated to Anhui Medical University, 17 Lujiang Road, Hefei, Anhui Province 230001, China
    J Clin Neurosci 19:121-7. 2012
    ..These findings provide a new insight into the biology of BTSC and open a new perspective for targeted therapy against the brain tumors...
  58. pmc Deimination of human filaggrin-2 promotes its proteolysis by calpain 1
    Chiung Yueh Hsu
    UMR5165 CNRS, F 31059 Toulouse, France
    J Biol Chem 286:23222-33. 2011
    ..Altogether, these data demonstrate that calpain 1 is essential for the proteolytic processing of FLG2 and that deimination accelerates this process...
  59. pmc The relevance of citrullinated vimentin in the production of antibodies against citrullinated proteins and the pathogenesis of rheumatoid arthritis
    Katleen Van Steendam
    Laboratory for Pharmaceutical Biotechnology, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium
    Rheumatology (Oxford) 50:830-7. 2011
    ....
  60. ncbi Doublecortin expression in the adult rat telencephalon
    J Nacher
    Laboratory of Neuroendocrinology, The Rockefeller University, New York, NY 10021, USA
    Eur J Neurosci 14:629-44. 2001
    ..Given these results, we hypothesize that DCX expression in differentiated neurons could be related to its capacity for microtubule reorganization and that this fact could be linked to axonal outgrowth or synaptogenesis...
  61. ncbi High levels of Cre expression in neuronal progenitors cause defects in brain development leading to microencephaly and hydrocephaly
    Paolo E Forni
    Department of Anatomy, Pharmacology, and Forensic Medicine, University of Turin, 10126 Turin, Italy
    J Neurosci 26:9593-602. 2006
    ..Furthermore, they underscore the potential risks for developmental studies of high levels of nuclear Cre in neurogenic cells...
  62. ncbi Nestin, a neuroectodermal stem cell marker molecule, is expressed in Leydig cells of the human testis and in some specific cell types from human testicular tumours
    Maria V T Lobo
    Departamento de Biología Celular y Genética, Universidad de Alcala, 28871 Alcala de Henares, Madrid, Spain
    Cell Tissue Res 316:369-76. 2004
    ..Thus, nestin is expressed in a population of normal and hyperplastic Leydig cells and in Sertoli cells in the presence of intratubular germ-cell neoplasia. Nestin may be a good marker for identifying components of testicular teratomas...
  63. ncbi Anterior-posterior patterning of neural differentiated embryonic stem cells by canonical Wnts, Fgfs, Bmp4 and their respective antagonists
    Marijke Hendrickx
    Vrije Universiteit Brussel VUB, Lab for Cell Genetics, B 1050 Brussels, Belgium
    Dev Growth Differ 51:687-98. 2009
    ..These data confirm that in the mouse, two steps are involved in neural patterning and we show that while Fgf4, Fgf8 and Wnt1 have no strong patterning effect, Fgf2, Wnt3a and Bmp4 are strong posteriorizing factors...
  64. ncbi Nestin-linked green fluorescent protein transgenic nude mouse for imaging human tumor angiogenesis
    Yasuyuki Amoh
    AntiCancer, Inc, San Diego, California 92111, USA
    Cancer Res 65:5352-7. 2005
    ..These results suggest that this model is useful for the imaging of the angiogenesis of human as well as rodent tumors and visualization of the efficacy of angiogenetic inhibitors...
  65. pmc Nascent blood vessels in the skin arise from nestin-expressing hair-follicle cells
    Yasuyuki Amoh
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Proc Natl Acad Sci U S A 101:13291-5. 2004
    ..This model displays very early events in skin angiogenesis and can serve for rapid antiangiogenesis drug screening...
  66. pmc Nestin is a novel target for suppressing pancreatic cancer cell migration, invasion and metastasis
    Yoko Matsuda
    Department of Pathology, Nippon Medical School, Bunkyo ku, Tokyo, Japan
    Cancer Biol Ther 11:512-23. 2011
    ..Thus, nestin plays important roles in pancreatic cancer cell migration, invasion and metastasis by selectively modulating the expression of actin and cell adhesion molecules, and may therefore be a novel therapeutic target in PDAC...
  67. ncbi Barrier function in transgenic mice overexpressing K16, involucrin, and filaggrin in the suprabasal epidermis
    Richard B Presland
    J Invest Dermatol 123:603-6. 2004
  68. ncbi Filaggrin null mutations are associated with atopic dermatitis and elevated levels of IgE in the Japanese population: a family and case-control study
    Hisako Enomoto
    Department of Medical Genetics, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba, Ibaraki, Japan
    J Hum Genet 53:615-21. 2008
    ..The combined P value for the family and case-control data was significant for the S2554X and combined null mutations. Our data further support the importance of FLG in AD development...
  69. ncbi Aberrant nestin expression during ethylnitrosourea-(ENU)-induced neurocarcinogenesis
    Taichang Jang
    Department of Surgery Neurosurgery, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Neurobiol Dis 15:544-52. 2004
    ....
  70. pmc Genetic supplementation of RDS alleviates a loss-of-function phenotype in C214S model of retinitis pigmentosa
    May Nour
    Cell Biology, University of Oklahoma Health Sciences Center, 940 Stanton L Young Blvd, Oklahoma City, OK 73104, USA
    Adv Exp Med Biol 613:129-38. 2008
  71. pmc Flaky tail mouse denotes human atopic dermatitis in the steady state and by topical application with Dermatophagoides pteronyssinus extract
    Catharina Sagita Moniaga
    Department of Dermatology, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Am J Pathol 176:2385-93. 2010
    ..These results suggest that the Flg(ft) mouse genotype has potential as an animal model of AD corresponding with filaggrin mutation in human AD...
  72. ncbi Structure and function of hemidesmosomes: more than simple adhesion complexes
    L Borradori
    Department of Dermatology, DHURDV, University Hospital of Geneva, Switzerland
    J Invest Dermatol 112:411-8. 1999
    ..The aims of this review are to highlight the recent progresses of our knowledge on the organization and assembly of hemidesmosomes, their involvement in signaling pathways as well as their participation in clinical pathologic conditions...
  73. ncbi Mutations in the filaggrin gene in Han Chinese patients with atopic dermatitis
    H Zhang
    Department of Dermatology, Xinhua Hospital, Shanghai, China
    Allergy 66:420-7. 2011
    ..This study aimed to investigate the role of FLG mutations in AD and AD-associated phenotypes in a Chinese population...
  74. ncbi Peripherin and ATF3 genes are differentially regulated in regenerating and non-regenerating primary sensory neurons
    Adam J Reid
    Blond McIndoe Research Laboratories, Tissue Injury and Repair Group, University of Manchester, UK
    Brain Res 1310:1-7. 2010
    ..The gene expression of the structural neurofilament NF-H is found to be significantly downregulated following injury in both sensory subpopulations...
  75. ncbi FLG mutation p.Lys4021X in the C-terminal imperfect filaggrin repeat in Japanese patients with atopic eczema
    I Nemoto-Hasebe
    Department of Dermatology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Br J Dermatol 161:1387-90. 2009
    ..Mutations in the gene encoding filaggrin (FLG) have been shown to predispose to atopic eczema (AE)...
  76. ncbi Axonal integrity in the absence of functional peroxisomes from projection neurons and astrocytes
    Astrid Bottelbergs
    Laboratory of Cell Metabolism, Department of Pharmaceutical Sciences, K U Leuven, B 3000 Leuven, Belgium
    Glia 58:1532-43. 2010
    ..Lack of peroxisomal metabolism in astrocytes causes increased VLCFA levels in myelin, but this has no major impact on neurological functioning...
  77. ncbi FLG mutations in ichthyosis vulgaris and atopic eczema: spectrum of mutations and population genetics
    M Akiyama
    Department of Dermatology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Br J Dermatol 162:472-7. 2010
    ..A restoration of the barrier function seems a feasible and promising strategy for treatment and prevention in individuals with filaggrin deficiency...
  78. pmc Filaggrin haploinsufficiency is highly penetrant and is associated with increased severity of eczema: further delineation of the skin phenotype in a prospective epidemiological study of 792 school children
    S J Brown
    Department of Dermatology, Royal Victoria Infirmary, Newcastle upon Tyne, UK
    Br J Dermatol 161:884-9. 2009
    ..Null mutations within the filaggrin gene (FLG) cause ichthyosis vulgaris and are associated with atopic eczema. However, the dermatological features of filaggrin haploinsufficiency have not been clearly defined...
  79. ncbi Distribution of nestin immunoreactivity in the normal adult human forebrain
    Huaiyu Gu
    Department of Neurobiology and Anatomy, Sun Yat Sen University of Medical Sciences, 74 Zhongshan 2nd Road, Guangzhou 510089, PR China
    Brain Res 943:174-80. 2002
    ..These data indicate that nestin exist in the adult human brain outside of the subependymal zone and dentate gyrus and also implies that nestin-immunoreactive cells may play a role in the modulation of basal forebrain function...
  80. ncbi A comparison of intermediate filament markers for presumptive astroglia in the developing rat neocortex: immunostaining against nestin reveals more detail, than GFAP or vimentin
    M Kalman
    Department of Anatomy, Histology and Embryology, Semmelweis University of Medicine, Tuzoltó 58, 1094, Budapest, Hungary
    Int J Dev Neurosci 19:101-8. 2001
    ..Nestin immunostaining is therefore highly recommended for studies of the glial architecture in the early post-natal brain development...
  81. ncbi Loss-of-function variants of the filaggrin gene are associated with atopic eczema and associated phenotypes in Swedish families
    Elisabeth Ekelund
    Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden
    Acta Derm Venereol 88:15-9. 2008
    ..Our results support an important role for the filaggrin gene variants R501X and 2282del4 in the development and severity of atopic eczema and indicate a possible role for the subsequent progression into eczema-associated phenotypes...
  82. ncbi Optic nerve lesion increases cell proliferation and nestin expression in the adult mouse eye in vivo
    Stefanie G Wohl
    Department of Experimental Neurology, University of Jena Medical School, Erlanger Allee 101, 07747 Jena, Germany
    Exp Neurol 219:175-86. 2009
    ..Since reactive glia cells can participate in adult neuro- and gliogenesis this may indicate a potential for regeneration after ON lesion in vivo...
  83. pmc Nestin is essential for zebrafish brain and eye development through control of progenitor cell apoptosis
    Hua Ling Chen
    Institute of Cellular and System Medicine, National Health Research Institutes, Zhunan, Taiwan
    PLoS ONE 5:e9318. 2010
    ..Nestin is expressed in neural progenitor cells (NPC) of developing brain. Despite its wide use as an NPC marker, the function of nestin in embryo development is unclear...
  84. ncbi The bacterial cytoskeleton: an intermediate filament-like function in cell shape
    Nora Ausmees
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Cell 115:705-13. 2003
    ..We propose that IF-like filaments of crescentin assemble into a helical structure, which by applying its geometry to the cell, generates a vibrioid or helical cell shape depending on the length of the cell...
  85. pmc Coordinate interaction between IL-13 and epithelial differentiation cluster genes in eosinophilic esophagitis
    Carine Blanchard
    Division of Allergy and Immunology, Department of Pediatrics, Cincinnati Children s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA
    J Immunol 184:4033-41. 2010
    ..These results establish that the epithelial response in EE involves a cooperative interaction between IL-13 and expression of EDC genes...
  86. pmc Hedgehog signaling is required for effective regeneration of exocrine pancreas
    Volker Fendrich
    Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Gastroenterology 135:621-31. 2008
    ..Given the known role of Hh signaling in exocrine pancreatic cancer, these findings may provide a mechanistic link between injury-induced activation of pancreatic progenitors and subsequent pancreatic neoplasia...
  87. ncbi Derivation and large-scale expansion of multipotent astroglial neural progenitors from adult human brain
    Noah M Walton
    Department of Neuroscience, McKnight Brain Institute, University of Florida, Gainesville, FL 32610, USA
    Development 133:3671-81. 2006
    ..These findings suggest an unprecedented degree of inducible plasticity is retained by cells of the adult central nervous system...
  88. ncbi SOX9 and SOX10 but not BRN2 are required for nestin expression in human melanoma cells
    Anna Flammiger
    Department of Dermatology and Allergology, Ludwig Maximilian University, Munich, Germany
    J Invest Dermatol 129:945-53. 2009
    ..Downregulation of SOX9 and of SOX10 markedly decreased nestin levels in melanoma cells in a cooperative manner. Thus, SOX9 and SOX10 but not BRN2 seem to be required for nestin expression in human melanoma...
  89. ncbi Two common loss-of-function mutations within the filaggrin gene predispose for early onset of atopic dermatitis
    Susanne Stemmler
    J Invest Dermatol 127:722-4. 2007
  90. ncbi Neural stem cell markers, nestin and musashi proteins, in the progression of human glioma: correlation of nestin with prognosis of patient survival
    Tadej Strojnik
    Department of Neurosurgery, Maribor Teaching Hospital, SI 2000 Maribor, Slovenia
    Surg Neurol 68:133-43; discussion 143-4. 2007
    ..Their expression in glial tumors was evaluated by immunohistochemistry, and the histopathological scores correlated with levels of cysteine cathepsins that are known prognostic markers in several tumors...
  91. pmc Dynamic contribution of nestin-expressing stem cells to adult neurogenesis
    Diane C Lagace
    Department of Psychiatry, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9070, USA
    J Neurosci 27:12623-9. 2007
    ....
  92. pmc Nestin as a diagnostic and prognostic marker: immunohistochemical analysis of its expression in different tumours
    J Ehrmann
    J Clin Pathol 58:222-3. 2005
    ..Because of the weak and heterogeneous expression of nestin in neurinomas, phaeochromocytomas, and carcinoids, nestin detection in these lesions is of little practical use...
  93. ncbi Filaggrin's fuller figure: a glimpse into the genetic architecture of atopic dermatitis
    Aileen Sandilands
    Epithelial Genetics Group, Human Genetics Unit, University of Dundee, Dundee, UK
    J Invest Dermatol 127:1282-4. 2007
    ..The recent publication of a strategy to sequence this difficult gene identifies a spectrum of both prevalent and rare mutations that collectively have a significant impact on susceptibility to atopic disease...
  94. ncbi Loss-of-function mutations in the gene encoding filaggrin cause ichthyosis vulgaris
    Frances J D Smith
    Epithelial Genetics Group, Human Genetics Unit, Division of Pathology and Neuroscience, University of Dundee, Ninewells Hospital and Medical School, Dundee DD1 9SY, UK
    Nat Genet 38:337-42. 2006
    ..The resultant matrix is cross-linked to form a major component of the cornified cell envelope. We find that loss or reduction of this major structural protein leads to varying degrees of impaired keratinization...
  95. pmc Roles for the stem cell associated intermediate filament Nestin in prostate cancer migration and metastasis
    Wolfram Kleeberger
    Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Cancer Res 67:9199-206. 2007
    ..Further dissection of this novel Nestin migration pathway may lead to strategies to prevent and neutralize metastatic spread...
  96. ncbi Two distinct subpopulations of nestin-positive cells in adult mouse dentate gyrus
    Satoshi Fukuda
    Department of Integrated Biosciences, University of Tokyo, Kashiwa 277 8562, Japan
    J Neurosci 23:9357-66. 2003
    ....
  97. ncbi Inactivation of the glial fibrillary acidic protein gene, but not that of vimentin, improves neuronal survival and neurite growth by modifying adhesion molecule expression
    V Menet
    Institut National de la Santé et de la Recherche Médicale U336, Universite Montpellier II, F 34095 Montpellier, France
    J Neurosci 21:6147-58. 2001
    ..To conclude, our results suggest that GFAP expression is able to modulate key biochemical properties of astrocytes that are implicated in their permissivity...
  98. ncbi Risk analysis of early childhood eczema
    Hans Bisgaard
    Copenhagen Prospective Studies on Asthma in Childhood, Danish Pediatric Asthma Center, Health Sciences, University of Copenhagen, Copenhagen University Hospital, Gentofte, Copenhagen, Denmark
    J Allergy Clin Immunol 123:1355-60.e5. 2009
    ..The increasing prevalence of eczema suggests the role of environmental factors triggering a genetic predisposition...
  99. pmc Notch signaling enhances nestin expression in gliomas
    Alan H Shih
    Department of Cancer Biology and Genetics, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Neoplasia 8:1072-82. 2006
    ..However, activation of Notch alone is unable to induce this cellular expansion. These data suggest that Notch may have a contributing role in the stem-like character of glioma cells...
  100. ncbi Targeted ablation of Arnt in mouse epidermis results in profound defects in desquamation and epidermal barrier function
    Songmei Geng
    Department of Dermatology, Columbia University, New York, NY, USA
    J Cell Sci 119:4901-12. 2006
    ..The functional defects in Arnt-null mouse epidermis underscore the crucial role of Arnt in the maintenance of epidermal homeostasis, especially during the perinatal transition to the ex utero environment...
  101. doi Loss-of-function mutations in the filaggrin gene lead to reduced level of natural moisturizing factor in the stratum corneum
    Sanja Kezic
    J Invest Dermatol 128:2117-9. 2008

Research Grants63

  1. INTERACTIONS BETWEEN INTERMEDIATE FILAMENTS AND NUCLEUS
    Robert D Goldman; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Lamins are intermediate filament proteins that are found in the nuclear lamina and in the nucleoplasm...
  2. Regulation and function of nuclear envelope proteins in myelopoiesis and leukemia
    PETER CHARLES WIGRAM GAINES; Fiscal Year: 2010
    ..characteristic of Pelger-Hukt anomaly, and macrophages express certain nuclear envelope proteins and intermediate filament proteins called A-type lamins that are essentially lacking in other hematopoietic cells...
  3. Regulation and Catalysis of Human Insulin Degrading Enzyme
    Wei Jen Tang; Fiscal Year: 2013
    ..basis for the regulation of IDE by dimerization, physiologically relevant cellular factors such as intermediate filament proteins, nestin and vimentin, and by posttranslational modifications such as phosphorylation and acetylation...
  4. Mechanisms of Genetic Reversion in Ichthyosis With Confetti
    Keith A Choate; Fiscal Year: 2013
    ..We have found that mutations affecting the carboxy terminal non-helical domains of the intermediate filament proteins keratin 1 and keratin 10 (KRT1, KRT10) lead to their mis-localization to the nucleus and cause ..
  5. MECHANISMS RESPONSIBLE FOR GENOMIC INSTABILITY IN PREMATURE AGING LAMINOPATHIES
    SUSANA GONZALO HERVAS; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): A-type lamins are intermediate filament proteins that provide a scaffold for tethering chromatin and protein complexes regulating nuclear structure and function...
  6. The Nucleocytoskeleton in Progeria and Aging
    Howard J Worman; Fiscal Year: 2013
    ..HGPS is caused by mutations in the LMNA gene, which encodes the A-type nuclear lamins, intermediate filament proteins associated with the inner nuclear membrane...
  7. REQUIREMENT FOR SENSITIVE T CELL RESPONSE TO ANTIGEN
    MICHAEL LORAN DUSTIN; Fiscal Year: 2013
    ..In the context of regulatory T cells, we will study the role of intermediate filament proteins. These studies will lead to a greater understanding of sensitivity to self and foreign antigen in ..
  8. Discovery of Novel Autoantigens in Patients with Inclusion Body Myositis
    KEVIN C O'CONNOR; Fiscal Year: 2013
    ..recombinant immunoglobulin derived from these single cells will be tested for reactivity to muscle intermediate filament proteins and other candidate autoantigens...
  9. Keratin sumoylation and its function during hepatocyte stress and liver disease
    NATASHA TASHEVA SNIDER; Fiscal Year: 2013
    ..The candidate's long-term career goals are to become an expert on intermediate filament proteins and their roles in human diseases, obtain a tenure-track faculty position and become a successful ..
  10. Assembly and Axonal Transport of Neurofilaments
    Anthony Brown; Fiscal Year: 2013
    ..We will also extend our studies to other types of intermediate filament proteins to establish whether our findings are more generally applicable...
  11. Nuclear Lamin Functions and Human Disease
    Brian K Kennedy; Fiscal Year: 2013
    ..progeroid syndromes to tissue-specific dystrophies to cancer, indicating that this class of nuclear intermediate filament proteins plays a critical role in the maintenance of tissue homeostasis...
  12. Pathogenesis of Emery-Dreifuss Muscular Dystrophy
    Howard J Worman; Fiscal Year: 2013
    ..LMNA encodes A-type nuclear lamins, intermediate filament proteins associated with the inner nuclear membrane...
  13. Control of Mitotic Entry by Regulators of Cdc2
    SALLY A KORNBLUTH; Fiscal Year: 2010
    ..requires phosphorylation of both Cdc25 T138 (which reduces the affinity of 14-3-3 for Cdc25) and intermediate filament proteins (which then bind 14-3-3, thereby serving as an abundant 14-3-3 "sink")...
  14. PATHOBIOLOGY OF THE NUCLEAR ENVELOPE IN CANCER
    Howard Worman; Fiscal Year: 2000
    ..The experiments in the third specific aim will focus on the nuclear lamins, intermediate filament proteins of the nuclear lamina Because lamins A and C, which arise from the same gene by altemative splicing, ..
  15. INTERFERON EFFECTS ON INTERMEDIATE FILAMENTS IN TUMOR CE
    DIANNE BUCKMAN; Fiscal Year: 1992
    ..This will be accomplished by studying the effect of IFN on the expression of intermediate filament proteins and genes, specifically, K8 and K18 in normal and transformed human cells...
  16. Intermediate filament cytoskeleton and cell shape in Caulobacter crescentus
    Christine Jacobs Wagner; Fiscal Year: 2009
    ..blistering and muscular dystrophy to lipodystrophy and premature aging, are caused by abnormalities in intermediate filament proteins. This study of crescentin, a bacterial version of an intermediate filament protein, is likely to ..
  17. Molecular mechanisms of airway smooth muscle function
    Dale Tang; Fiscal Year: 2007
    ..Pilot studies indicate that intermediate filament proteins may play a role in mediating force transmission and contraction in airway smooth muscle...
  18. DEVELOPING AND ADULT VISUAL SYSTEM
    Steven Fisher; Fiscal Year: 1999
    ..Specifically we will determine: 1) when the upregulation of intermediate filament proteins and the downregulation of enzymatic and vitamin A binding proteins occurs in Muller cells (by using ..
  19. CHARACTERIZATION OF HAIRLESS ASSOCIATED PROTEINS
    Karima Djabali; Fiscal Year: 2004
    ..will characterize the cell-types expressing hairless protein in the mouse brain using the different intermediate filament proteins as specific markers...
  20. NEUROONCOGENESIS BY RESORPTIVE CARCINOGENS
    Adalbert Koestner; Fiscal Year: 1990
    ..Synthesis and phosphorylation of tubulin, intermediate filament proteins, actin and their associated proteins will be examined in T9 cells during exposure to NGF and GMF as ..
  21. BIOMEDICAL RESEARCH SUPPORT
    Stanley Fowler; Fiscal Year: 1991
    ..2) Site specific immunolabeling of purified intermediate filament proteins will be studied using quick frozen, deep etched preparations in order to clarify the structure of ..
  22. Keratin Function in the Cancer Cell
    Daryle DePianto; Fiscal Year: 2007
    ..The main objective of this proposal is to determine how intermediate filament proteins contribute to the cancer phenotype. Utilizing a transgenic mouse model for basal cell carcinoma (BCC...
  23. VISUAL SYSTEM SPECIFIC PROTEINS--BIOCHEMICAL STUDIES
    NISSON SCHECHTER; Fiscal Year: 1999
    ..with the goldfish the capacity for optic nerve regeneration and has an identical distribution of intermediate filament proteins. However, the zebrafish is amenable to genetic and transgenic manipulations that allow easy ..
  24. DIFFERENTIATION AND STROMA-INDUCTION IN NEURAL TUMORS
    Robert Carey; Fiscal Year: 1990
    ..2) The expression of structural and non-structural markers of differentiation, including intermediate filament proteins (glial, neuronal), enzymatic cytoplasmic proteins (neuron-specific enolase), and cell surface membrane ..
  25. Junctions, Cytoskeleton & Matrix of the Oral Epithelium
    Jonathan Jones; Fiscal Year: 2006
    ..surface interaction in oral epithelial cells" will test the hypothesis that changes in expression of intermediate filament proteins regulates cell migration and tumor cell metastasis...
  26. ANTIGEN-INDUCED CELL TYPE SPECIFIC TUMORS
    NICHOLAS COWAN; Fiscal Year: 1992
    ..For these studies, we will use the genes encoding three nervous-system specific intermediate filament proteins: the glial filament acidic protein (GFAP) and two neurofilament (NF) proteins (NF68kd and FF150kd)...
  27. Analysis of transcription in lens using tiled microarrays (ChIP on chip)
    Ales Cvekl; Fiscal Year: 2007
    ..These loci will include all lens structural genes (crystallins, membrane and intermediate filament proteins), key genes regulating lens lineage formation (e.g...
  28. DUX4 and Facioscapulohumeral Muscular Dystrophy
    Howard Worman; Fiscal Year: 2004
    ..This work could lead to the development of new diagnostic methods as well as the identification of potential protein targets for the treatment of FSHD. ..
  29. Lamin A in Adipocyte Differentation and Survival
    Howard Worman; Fiscal Year: 2004
    ..This finding implicates lamins A and C, intermediate filament proteins of the nuclear envelope, as part of a novel pathway involved in the control of body fat distribution...
  30. Lamin A Mutation and Hutchinson-Gilford Progeria
    Howard Worman; Fiscal Year: 2008
    ..aging, is caused by a dominant de novo mutation in LMNA, the gene that encodes lamins A and C, intermediate filament proteins associated with the nuclear envelope...
  31. HEMIDESMOSOMAL PROTEIN LINKAGE MAP BY TWO-HYBRID SYSTEM
    Sirpa Aho; Fiscal Year: 2002
    ..abstract_text> ..
  32. Protease-Mediated Events in Epidermal Differentiation
    RICHARD PRESLAND; Fiscal Year: 2006
    ..abstract_text> ..
  33. PROFILAGGRIN IN EPIDERMAL DIFFERENTIATION AND FUNCTION
    RICHARD PRESLAND; Fiscal Year: 2002
    ....
  34. Orthotopic models of tumor angiogenesis and blood flow
    Meng Yang; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  35. 2002 Gordon Research Conf. on Intermediate Filaments
    PIERRE COULOMBE; Fiscal Year: 2002
    ..In addition, there will be a special "Perspectives" session and a platform session dedicated to the discussion of posters. ..
  36. Keratin proteins and non-melanoma skin tumors
    PIERRE COULOMBE; Fiscal Year: 2008
    ..Like other keratins and all other intermediate filament proteins, K17 provides structural support...
  37. RHOMOCYCSTEINASE FOR HOMOCYSTEINE ASSAY
    Yuying Tan; Fiscal Year: 2002
    ..The tHCY enzymatic kits for these applications will be ready for commercial launch at this point. PROPOSED COMMERCIAL APPLICATION: Not Available ..
  38. Adenoviral GFP targeting of metastatic human tumors using multiple delivery route
    Hiroyuki Kishimoto; Fiscal Year: 2008
    ..Having obtained these results, we can move forward in the Phase II grant application with experiments to bring OBP-401 to the clinic for use in cancer surgery in human patients. [unreadable] [unreadable] [unreadable]..
  39. Muller glia and neuronal regeneration in the retina
    Andy Fischer; Fiscal Year: 2009
    ..This information could be applied to not only stimulate neural regeneration to treat sight-threatening diseases of the retina, but also to control gliosis that may be detrimental to the pathogenesis of retinal disorders. ..
  40. Screen for Small Molecule Inhibitors of Nuclear Protein Import
    Larry Gerace; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  41. Therapeutic hair follicle-derived neurospheres
    Meng Yang; Fiscal Year: 2007
    ..Human hair- follicle bulge cells will be further characterized and developed for therapeutic potential for nerve regeneration in Phase III. [unreadable]..
  42. Enzyme therapy for hyperhomocysteinemia
    Yuying Tan; Fiscal Year: 2005
    ..Phase II results will lead to an IND for MEGC-PEG-rMETase for end stage renal disease patients and other patients with intractable hyperhomocysteinemia and high cardiovascular disease mortality. ..
  43. Transfection of Lens Cells with Phakinin Mutants
    MARK IRELAND; Fiscal Year: 2006
    ..The results of these studies will lead to a better understanding of beaded filament function and the etiology of some forms of cataract. ..
  44. Regulation of neuronal stem cell death
    Erhard Bieberich; Fiscal Year: 2006
    ..In conclusion, this study will identify mechanisms for regulation of apoptosis by ceramide that are critical for normal brain development and/or the etiology of subsequent, pathological disorders in adulthood. ..
  45. Inhibitors of 5alpha-reductase for acne therapy
    Lingna Li; Fiscal Year: 2002
    ..PROPOSED COMMERCIAL APPLICATIONS: Liposomal 4-MA will be developed as a topical selectively targeted therapeutic for acne for which they should be a very market. ..
  46. Imageable tumor-targeting bacteria
    Ming Zhao; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  47. FEI Morgagni Transmission Electron Microscope
    Andrew Goldberg; Fiscal Year: 2003
    ..on an as-available basis, with priority given to NIH-funded projects Oakland University (OU) recognizes the immediate need for this instrument and has committed appropriate space and financial support for ongoing maintenance costs ..
  48. Discovery of novel fluorescent reporter genes
    Ming Zhao; Fiscal Year: 2004
    ..Reporters with spectral properties that can be used for whole-body imaging of tumors in the lung and their metastases will be candidates for further development for numerous applications of multi-color imaging. ..
  49. Import of Adenovirus DNA into the nucleus
    Larry Gerace; Fiscal Year: 2007
    ..Finally, it could provide the basis for developing efficient means for nonviral gene transfer, which would be useful for gene therapy and functional studies of cells. ..
  50. Targeted tumoricidal bacteria
    Ming Zhao; Fiscal Year: 2007
    ..Future human trials of the tumor-killing bacteria can be held after the Phase I and Phase II grant periods are completed. [unreadable] [unreadable] [unreadable]..
  51. Dual-color tumor-host imaging models
    Meng Yang; Fiscal Year: 2007
    ..These models have significant commercial potential for discovery and development of stroma-targeted and anti-angiogenesis drugs. [unreadable] [unreadable] [unreadable]..
  52. MECHANISMS OF NUCLEAR IMPORT
    Larry Gerace; Fiscal Year: 2006
    ..Thus, understanding basic nuclear transport mechanisms can provide a framework for developing new therapeutic approaches relevant to viral pathogenesis, cancer and immunity and other processes. ..
  53. BDNF PROMOTER ELEMENTS IN THE DEVELOPING HYPOTHALAMUS
    STEVEN KERNIE; Fiscal Year: 2005
    ..In this manner they can determine at a molecular level the function of the targeted promoter. ..
  54. Clinical Approaches to Ileal Pouch Dysfunction
    Bo Shen; Fiscal Year: 2005
    ..The AIMS of study are to 1) investigate visceral hypersensitivity using barostat examination of the pouch; 2) conduct a randomized, placebo-controlled clinical trial evaluating the use of amitriptyline in IPS. ..
  55. An Ets2 dependent stromal restriction of breast cancer
    Robert Oshima; Fiscal Year: 2007
    ..Finally, the importance of hematopoietic derived cells for tumor angiogenesis will be investigated by reciprocal bone marrow transplantation studies. ..
  56. DEVELOPMENTAL CONTROL OF CYTOKERATIN EXPRESSION
    Robert Oshima; Fiscal Year: 2002
    Keratins 8 (K8) and 18 (K18) are the first intermediate filament proteins to be expressed during mouse and human development and are subsequently restricted to a wide variety of internal, simple epithelial tissues...
  57. Lens plasma membrane-cytokeleton in selenite cataract
    CHARLES FLESCHNER; Fiscal Year: 2003
    ..Also, these studies will describe changes in the cytoskeletal network which are associated with the formation of cataracts. It is anticipated this information will be useful in the understanding of human senile cataract. ..
  58. MOLECULAR ANALYSIS OF OCULAR LENS BEADED FILAMENTS
    Paul G FitzGerald; Fiscal Year: 2010
    ..In the absence of these proteins the lens undergoes a loss of organization that leads to a loss of optical clarity that gets progressively worse with age. ..
  59. 2006 Gordon Research Conference on Intermediate Filaments
    M Omary; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  60. WNT signals in developing and postnatal teeth
    Sarah Millar; Fiscal Year: 2008
    ..These experiments will help to place WNT signals in the network of regulatory factors that control tooth development, and will test the potential use of WNT activation in strategies for tooth or enamel regeneration. ..
  61. Regulating mouse neural induction
    KATHY O SHEA; Fiscal Year: 2004
    ..abstract_text> ..
  62. Chemoprevention of IBD-Associated Colon Cancer
    Laura P Hale; Fiscal Year: 2010
    ..The proposed studies will further elucidate mechanisms that can lead to novel interventions to prevent IBD-associated colon cancers, providing great benefit to IBD patients and their families. ..