phosphoenolpyruvate sugar phosphotransferase system

Summary

Summary: The bacterial sugar phosphotransferase system (PTS) that catalyzes the transfer of the phosphoryl group from phosphoenolpyruvate to its sugar substrates (the PTS sugars) concomitant with the translocation of these sugars across the bacterial membrane. The phosphorylation of a given sugar requires four proteins, two general proteins, Enzyme I and HPr and a pair of sugar-specific proteins designated as the Enzyme II complex. The PTS has also been implicated in the induction of synthesis of some catabolic enzyme systems required for the utilization of sugars that are not substrates of the PTS as well as the regulation of the activity of adenylate cyclase. EC 2.7.1.-.

Top Publications

  1. pmc How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria
    Josef Deutscher
    Microbiologie et Genetique Moleculaire, INRA CNRS INA PG UMR 2585, Thiverval Grignon, France
    Microbiol Mol Biol Rev 70:939-1031. 2006
  2. pmc Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria
    P W Postma
    E C Slater Institute, University of Amsterdam, The Netherlands
    Microbiol Rev 57:543-94. 1993
  3. ncbi The mechanisms of carbon catabolite repression in bacteria
    Josef Deutscher
    Laboratorie de Microbiologie et Génétique Moléculaire, INRA AgroParisTech CNRS, F 78850 Thiverval Grignon, France
    Curr Opin Microbiol 11:87-93. 2008
  4. pmc Metabolic engineering for the production of shikimic acid in an evolved Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system
    Adelfo Escalante
    Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico UNAM, Av, Universidad 2001, Col, Chamilpa, Cuernavaca, Morelos, 62210, Mexico
    Microb Cell Fact 9:21. 2010
  5. pmc Consequences of phosphoenolpyruvate:sugar phosphotranferase system and pyruvate kinase isozymes inactivation in central carbon metabolism flux distribution in Escherichia coli
    Eugenio Meza
    Cellular Engineering and Biocatalysis Department, Biotechnology Institute, Universidad Nacional Autonoma de Mexico, Apdo Postal 510 3, Cuernavaca, Morelos 62210, Mexico
    Microb Cell Fact 11:127. 2012
  6. ncbi Induction of the Bacillus subtilis ptsGHI operon by glucose is controlled by a novel antiterminator, GlcT
    J Stülke
    Unité de Biochimie Microbienne, Institut Pasteur, URA 1300 du Centre National de la Recherche Scientifique, Paris, France
    Mol Microbiol 25:65-78. 1997
  7. ncbi Expression of ptsG, the gene for the major glucose PTS transporter in Escherichia coli, is repressed by Mlc and induced by growth on glucose
    J Plumbridge
    Institut de Biologie Physico Chimique UPR9073, Paris, France
    Mol Microbiol 29:1053-63. 1998
  8. pmc The PTS transporters of Lactobacillus gasseri ATCC 33323
    Alyssa L Francl
    Department of Food Science and Human Nutrition, University of Illinois, 905 S, Goodwin Ave, Urbana, IL, USA
    BMC Microbiol 10:77. 2010
  9. pmc Seryl-phosphorylated HPr regulates CcpA-independent carbon catabolite repression in conjunction with PTS permeases in Streptococcus mutans
    Lin Zeng
    Department of Oral Biology, University of Florida, Gainesville, FL 32610, USA
    Mol Microbiol 75:1145-58. 2010
  10. pmc Lipoarabinomannan biosynthesis in Corynebacterineae: the interplay of two α(1→2)-mannopyranosyltransferases MptC and MptD in mannan branching
    Arun K Mishra
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    Mol Microbiol 80:1241-59. 2011

Detail Information

Publications236 found, 100 shown here

  1. pmc How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria
    Josef Deutscher
    Microbiologie et Genetique Moleculaire, INRA CNRS INA PG UMR 2585, Thiverval Grignon, France
    Microbiol Mol Biol Rev 70:939-1031. 2006
    ..As we shall see, the PTS regulation network not only controls carbohydrate uptake and metabolism but also interferes with the utilization of nitrogen and phosphorus and the virulence of certain pathogens...
  2. pmc Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria
    P W Postma
    E C Slater Institute, University of Amsterdam, The Netherlands
    Microbiol Rev 57:543-94. 1993
    ..In gram-positive bacteria, it has been demonstrated that HPr can be phosphorylated by ATP on a serine residue and this modification may perform a regulatory function...
  3. ncbi The mechanisms of carbon catabolite repression in bacteria
    Josef Deutscher
    Laboratorie de Microbiologie et Génétique Moléculaire, INRA AgroParisTech CNRS, F 78850 Thiverval Grignon, France
    Curr Opin Microbiol 11:87-93. 2008
    ..PTS-independent CCR mechanisms are operative in several other bacteria...
  4. pmc Metabolic engineering for the production of shikimic acid in an evolved Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system
    Adelfo Escalante
    Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico UNAM, Av, Universidad 2001, Col, Chamilpa, Cuernavaca, Morelos, 62210, Mexico
    Microb Cell Fact 9:21. 2010
    ..Derivatives of PB12 strain were constructed to determine the effects of inactivating aroK, aroL, pykF or pykA and the expression of plasmid-coded genes aroGfbr, tktA, aroB and aroE, on SA synthesis...
  5. pmc Consequences of phosphoenolpyruvate:sugar phosphotranferase system and pyruvate kinase isozymes inactivation in central carbon metabolism flux distribution in Escherichia coli
    Eugenio Meza
    Cellular Engineering and Biocatalysis Department, Biotechnology Institute, Universidad Nacional Autonoma de Mexico, Apdo Postal 510 3, Cuernavaca, Morelos 62210, Mexico
    Microb Cell Fact 11:127. 2012
    ..To characterize the effects of these modifications on cell physiology, carbon flux distribution and aromatics production capacity were determined...
  6. ncbi Induction of the Bacillus subtilis ptsGHI operon by glucose is controlled by a novel antiterminator, GlcT
    J Stülke
    Unité de Biochimie Microbienne, Institut Pasteur, URA 1300 du Centre National de la Recherche Scientifique, Paris, France
    Mol Microbiol 25:65-78. 1997
    ..A deletion of the terminator alleviates the need for GlcT. The activity of GlcT is negatively regulated by the glucose permease...
  7. ncbi Expression of ptsG, the gene for the major glucose PTS transporter in Escherichia coli, is repressed by Mlc and induced by growth on glucose
    J Plumbridge
    Institut de Biologie Physico Chimique UPR9073, Paris, France
    Mol Microbiol 29:1053-63. 1998
    ..The Mlc protein binds to two sites centred at -6 and -175 upstream of the major ptsG transcript. CAP binds at -40.5 compared with this site, typical of class II CAP-regulated promoters, and the binding of CAP and Mlc is co-operative...
  8. pmc The PTS transporters of Lactobacillus gasseri ATCC 33323
    Alyssa L Francl
    Department of Food Science and Human Nutrition, University of Illinois, 905 S, Goodwin Ave, Urbana, IL, USA
    BMC Microbiol 10:77. 2010
    ..The currently available annotation of the L. gasseri ATCC 33323 genome describes numerous putative genes involved in carbohydrate utilization, yet the specific functions of many of these genes remain unknown...
  9. pmc Seryl-phosphorylated HPr regulates CcpA-independent carbon catabolite repression in conjunction with PTS permeases in Streptococcus mutans
    Lin Zeng
    Department of Oral Biology, University of Florida, Gainesville, FL 32610, USA
    Mol Microbiol 75:1145-58. 2010
    ..Thus, in S. mutans, serine-phosphorylated HPr functions in concert with particular PTS permeases to prioritize carbohydrate utilization by modulating sugar transport and transcription of catabolic operons...
  10. pmc Lipoarabinomannan biosynthesis in Corynebacterineae: the interplay of two α(1→2)-mannopyranosyltransferases MptC and MptD in mannan branching
    Arun K Mishra
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    Mol Microbiol 80:1241-59. 2011
    ..Overall, our study enhances our understanding of complex lipoglycan biosynthesis in Corynebacterineae and sheds further light on the structural and functional relationship of these classes of polysaccharides...
  11. pmc Characterization of homologs of the small RNA SgrS reveals diversity in function
    Caryn S Wadler
    Department of Microbiology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Nucleic Acids Res 37:5477-85. 2009
    ..Genetic evidence suggests that a secondary structure in the E. coli SgrS 5' region inhibits sgrT translation. This structure is not present in S. typhimurium SgrS, which explains its higher level of SgrT production...
  12. ncbi Protein kinase-dependent HPr/CcpA interaction links glycolytic activity to carbon catabolite repression in gram-positive bacteria
    J Deutscher
    , CNRS, Lyon, France
    Mol Microbiol 15:1049-53. 1995
    ..The sensitivity of this complex formation to phosphorylation of HPr at His-15 also suggests a link between carbon catabolite repression and PTS transport activity...
  13. ncbi Adaptation for fast growth on glucose by differential expression of central carbon metabolism and gal regulon genes in an Escherichia coli strain lacking the phosphoenolpyruvate:carbohydrate phosphotransferase system
    Noemí Flores
    Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Apdo Postal 510 3, Cuernavaca, Morelos 62271, Mexico
    Metab Eng 7:70-87. 2005
    ..In PB12, glk, pgi, the TCA cycle and certain respiratory genes are also upregulated. A mutation in arcB in PB12 is apparently responsible for the upregulation of the TCA cycle and certain respiratory genes...
  14. ncbi A single V317A or V317M substitution in Enzyme II of a newly identified beta-glucoside phosphotransferase and utilization system of Corynebacterium glutamicum R extends its specificity towards cellobiose
    Pavel Kotrba
    Research Institute of Innovative Technology for the Earth, 9 2 Kizugawadai, Kizu, Soraku, Kyoto 619 0292, Japan
    Microbiology 149:1569-80. 2003
    ..Such results strengthen the evidence that the IIC domains can be regarded as selectivity filters of the PTS...
  15. pmc The Escherichia coli glucose transporter enzyme IICB(Glc) recruits the global repressor Mlc
    T W Nam
    School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 151 742, Korea
    EMBO J 20:491-8. 2001
    ..Therefore, the glucose induction of Mlc-regulated genes is caused by dephosphorylation of the membrane-bound transporter enzyme IICB(Glc), which directly recruits Mlc to derepress its regulon...
  16. ncbi Phylogeny of phosphoryl transfer proteins of the phosphoenolpyruvate-dependent sugar-transporting phosphotransferase system
    Kuang Yu Hu
    Division of Biology, University of California at San Diego, La Jolla 92093 0116, USA
    Res Microbiol 153:405-15. 2002
    ..Our findings define the evolutionary histories of these important bacterial proteins and provide guides for functional assignment of PTS-related proteins encoded by genes revealed by genome sequencing...
  17. ncbi Stimulation of the potassium sensor KdpD kinase activity by interaction with the phosphotransferase protein IIA(Ntr) in Escherichia coli
    Denise Lüttmann
    Georg August Universitat Gottingen, Abteilung für Allgemeine Mikrobiologie, Institut fur Mikrobiologie und Genetik, Grisebachstrasse 8, D 37077 Gottingen, Germany
    Mol Microbiol 72:978-94. 2009
    ..Taken together, the data show that the Ntr-PTS has an important role in maintaining K+ homeostasis and links K+ uptake to carbohydrate metabolism...
  18. ncbi Phosphoenolpyruvate phosphotransferase system and N-acetylglucosamine metabolism in Bacillus sphaericus
    Alejandro F Alice
    Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires 1428, Argentina
    Microbiology 149:1687-98. 2003
    ..sphaericus. They also open new possibilities for genetic improvements in industrial applications...
  19. pmc Global analysis of carbohydrate utilization by Lactobacillus acidophilus using cDNA microarrays
    Rodolphe Barrangou
    Genomic Sciences Graduate Program, North Carolina State University, Raleigh, NC 27695, USA
    Proc Natl Acad Sci U S A 103:3816-21. 2006
    ..L. acidophilus's adaptability to environmental conditions likely contributes to its competitive ability for limited carbohydrate sources available in the human gastrointestinal tract...
  20. ncbi The PTS(Ntr) system globally regulates ATP-dependent transporters in Rhizobium leguminosarum
    J Prell
    Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK
    Mol Microbiol 84:117-29. 2012
    ..ABC transport may be inactivated at low energy charge, conserving ATP for essential processes including K(+) homeostasis...
  21. ncbi The phosphotransferase protein EIIA(Ntr) modulates the phosphate starvation response through interaction with histidine kinase PhoR in Escherichia coli
    Denise Lüttmann
    Department of General Microbiology, Institute of Microbiology and Genetics, Georg August University, Grisebachstrasse 8, 37077 Gottingen, Germany
    Mol Microbiol 86:96-110. 2012
    ..This interaction increases the amount of phosphorylated response regulator PhoB. Thus, EIIA(Ntr) is an accessory protein that modulates the activities of two distinct sensor kinases, KdpD and PhoR, in E. coli...
  22. ncbi Metabolic engineering of Escherichia coli for improving L-3,4-dihydroxyphenylalanine (L-DOPA) synthesis from glucose
    Ana Joyce Muñoz
    Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Apdo Postal 510 3, 62210 Cuernavaca, Morelos, Mexico
    J Ind Microbiol Biotechnol 38:1845-52. 2011
    ..6 mg/g/h, L-DOPA yield from glucose of 51.7 mg/g and a final L-DOPA titer of 320 mg/l. In a batch fermentor culture in rich medium this strain produced 1.51 g/l of L-DOPA in 50 h...
  23. ncbi High-level resistance to class IIa bacteriocins is associated with one general mechanism in Listeria monocytogenes
    Anne Gravesen
    Department of Dairy and Food Science, Centre for Advanced Food Studies, LMC, The Royal Veterinary and Agricultural University, Rolighedsvej 30, DK 1958 Frederiksberg C, Denmark
    Microbiology 148:2361-9. 2002
    ..The changes in expression of the beta-glucoside-specific and the mannose-specific PTS are both influenced by this mechanism. The current understanding of the actual cause of class IIa resistance is discussed...
  24. pmc Interference of components of the phosphoenolpyruvate phosphotransferase system with the central virulence gene regulator PrfA of Listeria monocytogenes
    Sonja Mertins
    Lehrstuhl fur Mikrobiologie, Biozentrum, Universitat Wurzburg, Am Hubland, D 97074 Wurzburg, Germany
    J Bacteriol 189:473-90. 2007
    ..These data suggest that it is not the component(s) of the CCR or the common PTS pathway but, rather, the component(s) of subsequent steps that seem to be involved in the modulation of PrfA activity...
  25. pmc Class IIa bacteriocin resistance in Enterococcus faecalis V583: the mannose PTS operon mediates global transcriptional responses
    Mona Opsata
    Laboratory of Microbial Gene Technology and Food Microbiology, Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Norway
    BMC Microbiol 10:224. 2010
    ..To gain insight into bacteriocin resistance we studied mutants of E. faecalis V583 resistant to pediocin PA-1 by use of transcriptomic analyses...
  26. pmc Utilization of lactose and galactose by Streptococcus mutans: transport, toxicity, and carbon catabolite repression
    Lin Zeng
    Department of Oral Biology, University of Florida, College of Dentistry, P O Box 100424, Gainesville, FL 32610, USA
    J Bacteriol 192:2434-44. 2010
    ..Collectively, the results reveal a remarkably high degree of complexity in the regulation of lactose/galactose catabolism...
  27. ncbi Solution structure of the phosphoryl transfer complex between the signal-transducing protein IIAGlucose and the cytoplasmic domain of the glucose transporter IICBGlucose of the Escherichia coli glucose phosphotransferase system
    Mengli Cai
    Laboratory of Chemical Physics, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 278:25191-206. 2003
    ..IIBGlc and the two upstream complexes of the glucose phosphotransferase system (EI.HPr and IIAGlc.HPr) reveal a cascade in which highly overlapping binding sites on HPr and IIAGlc recognize structurally diverse proteins...
  28. pmc A novel role for enzyme I of the Vibrio cholerae phosphoenolpyruvate phosphotransferase system in regulation of growth in a biofilm
    Laetitia Houot
    Division of Infectious Disease, Children s Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA
    J Bacteriol 190:311-20. 2008
    ..As the PTS is highly conserved among bacteria, the enzyme I regulatory pathway may be relevant to a number of biofilm-based infections...
  29. ncbi Bacterial PEP-dependent carbohydrate: phosphotransferase systems couple sensing and global control mechanisms
    Joseph W Lengeler
    Max Planck Institut fur Dynamik komplexer technischer Systeme, Magdeburg, Germany
    Contrib Microbiol 16:65-87. 2009
    ..The analogy between the PTS and other prokaryotic systems, and more complex sensory systems from eukaryotic organisms which share elements with regulatory systems is obvious...
  30. ncbi Expression of ptsG encoding the major glucose transporter is regulated by ArcA in Escherichia coli
    Jin Young Jeong
    Laboratory of Macromolecular Interactions, School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 151 742, Korea
    J Biol Chem 279:38513-8. 2004
    ..These results suggest that the response regulator ArcA regulates expression of enzyme IICB(Glc) mediating the first step of glucose metabolism in response to the redox conditions of growth in E. coli...
  31. ncbi Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq
    Hiroshi Kawamoto
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Mol Microbiol 61:1013-22. 2006
    ..We also showed in vitro that SgrS forms a stable duplex with the ptsG mRNA, and that Hfq markedly facilitates the rate of duplex formation...
  32. pmc Multiple sugar: phosphotransferase system permeases participate in catabolite modification of gene expression in Streptococcus mutans
    Lin Zeng
    Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL 32610, USA
    Mol Microbiol 70:197-208. 2008
    ..Collectively, the results reveal the existence of a global regulatory network in S. mutans that governs the utilization of non-preferred carbohydrates in response to the availability and source of multiple preferred carbohydrates...
  33. ncbi Characterization of the individual glucose uptake systems of Lactococcus lactis: mannose-PTS, cellobiose-PTS and the novel GlcU permease
    Rute Castro
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Rua da Quinta Grande 6, Oeiras, Portugal
    Mol Microbiol 71:795-806. 2009
    ..This work completes the identification of the glucose transport systems in L. lactis MG1363...
  34. pmc A dual function for a bacterial small RNA: SgrS performs base pairing-dependent regulation and encodes a functional polypeptide
    Caryn S Wadler
    Department of Microbiology, University of Illinois at Urbana Champaign, 601 South Goodwin Avenue, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 104:20454-9. 2007
    ....
  35. pmc Transcriptional analysis of bglPH expression in Bacillus subtilis: evidence for two distinct pathways mediating carbon catabolite repression
    S Kruger
    Institut fur Mikrobiologie und Molekularbiologie, Ernst Moritz Arndt Universitat Greifswald, Germany
    J Bacteriol 178:2637-44. 1996
    ..Evidence that the antitermination protein LicT is a crucial element for this type of regulation is provided...
  36. pmc Complex phenotypic and genotypic responses of Listeria monocytogenes strains exposed to the class IIa bacteriocin sakacin P
    Girum Tadesse Tessema
    Nofima Mat AS, Osloveien 1, N 1430 Aas, Norway
    Appl Environ Microbiol 75:6973-80. 2009
    ..The great diversity among the resistant strains exposed to the same stress conditions suggests that there are different resistance mechanisms...
  37. pmc Keeping signals straight in transcription regulation: specificity determinants for the interaction of a family of conserved bacterial RNA-protein couples
    Oliver Schilling
    Department of General Microbiology, Institute of Microbiology and Genetics, Georg August University, Göttingen Germany
    Nucleic Acids Res 34:6102-15. 2006
    ....
  38. ncbi The complete phosphotranferase system in Escherichia coli
    J H Tchieu
    Department of Biology, University of California at San Diego, La Jolla 92093-0116, USA
    J Mol Microbiol Biotechnol 3:329-46. 2001
    ..All of these proteins are briefly described, and phylogenetic data for the major families are presented...
  39. pmc Three-dimensional structure of the Escherichia coli phosphocarrier protein IIIglc
    D Worthylake
    Department of Physics, University of Oregon, Eugene 97403
    Proc Natl Acad Sci U S A 88:10382-6. 1991
    ..The hydrophobic patch forms the primary crystal contact, suggesting a mode of association of IIIglc with other components of the phosphoenolpyruvate-dependent phosphotransferase system...
  40. ncbi Structural comparison of phosphorylated and unphosphorylated forms of IIIGlc, a signal-transducing protein from Escherichia coli, using three-dimensional NMR techniques
    J G Pelton
    Bone Research Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892
    Biochemistry 31:5215-24. 1992
    ..In the case of residues Ala-76, Asp-94, and Val-96, minor differences in NOEs, corresponding to interproton distances changes of less than 1.5 A, were observed.(ABSTRACT TRUNCATED AT 400 WORDS)..
  41. ncbi Evidence for direct interaction between enzyme I(Ntr) and aspartokinase to regulate bacterial oligopeptide transport
    N D King
    Department of Biochemistry and Center for Microbial Pathogenesis, State University of New York at Buffalo, Buffalo, New York 14214, USA
    J Biol Chem 276:21311-6. 2001
    ..The findings demonstrate a role for a PTS protein in the transport of a non-sugar solute and suggest an unusual regulatory function for aspartokinase in regulating the phosphorylation state of EI(Ntr)...
  42. ncbi Peptides as inhibitors of the first phosphorylation step of the Streptomyces coelicolor phosphoenolpyruvate: sugar phosphotransferase system
    Rosa Doménech
    Instituto de Biologia Molecular y Celular, Universidad Miguel Hernandez, Elche Alicante, Spain
    Biochemistry 51:7393-402. 2012
    ..Because the affinity of intact EIN(sc) for the whole HPr(sc) is 12 μM, we suggest that the assayed peptides might be considered as good hit compounds for inhibiting the interaction between HPr(sc) and EIN(sc)...
  43. ncbi Growth recovery on glucose under aerobic conditions of an Escherichia coli strain carrying a phosphoenolpyruvate:carbohydrate phosphotransferase system deletion by inactivating arcA and overexpressing the genes coding for glucokinase and galactose permeas
    Noemí Flores
    Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos, Mexico
    J Mol Microbiol Biotechnol 13:105-16. 2007
    ..However, the highest growth rate was obtained when glk and galP were overexpressed in the arcA(-) background. These results indicated that the arcA mutation enhanced glycolytic and respiratory capacities of the engineered strain...
  44. ncbi Topography of the surface of the signal-transducing protein EIIA(Glc) that interacts with the MalK subunits of the maltose ATP-binding cassette transporter (MalFGK2) of Salmonella typhimurium
    Bettina Blüschke
    Institut für Biologie Bakterienphysiologie, Humboldt Universitat zu Berlin, Chausseestr 117, D 10115 Berlin, Germany
    J Biol Chem 281:12833-40. 2006
    ..Moreover, a synthetic peptide encompassing residues 69-91 was demonstrated to partially inhibit ATPase activity. We also show for the first time that the N-terminal domain of EIIA(Glc) is essential for inducer exclusion...
  45. pmc Solution structure of the phosphoryl transfer complex between the signal transducing proteins HPr and IIA(glucose) of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system
    G Wang
    Laboratory of Chemical Physics, Building 5, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0510, USA
    EMBO J 19:5635-49. 2000
    ....
  46. pmc Solution structure of the N-terminal amphitropic domain of Escherichia coli glucose-specific enzyme IIA in membrane-mimetic micelles
    Guangshun Wang
    Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA
    Protein Sci 12:1087-96. 2003
    ....
  47. ncbi Transcription regulators potentially controlled by HPr kinase/phosphorylase in Gram-negative bacteria
    Gregory Boel
    Microbiologie et Genetique Moleculaire, CNRS UMR2585, Thiverval Grignon, France
    J Mol Microbiol Biotechnol 5:206-15. 2003
    ..We propose that HprK/P might control the phosphorylation state of HPr and EIIAs, which in turn could control the transcription regulators...
  48. ncbi The interplay of the EIIA(Ntr) component of the nitrogen-related phosphotransferase system (PTS(Ntr)) of Pseudomonas putida with pyruvate dehydrogenase
    Katharina Pflüger-Grau
    Centro Nacional de Biotecnologia, CSIC, Madrid, Spain
    Biochim Biophys Acta 1810:995-1005. 2011
    ..nitrogen. The metabolic targets of such a system are largely unknown...
  49. ncbi Effects of mutations and truncations on the kinetic behavior of IIAGlc, a phosphocarrier and regulatory protein of the phosphoenolpyruvate phosphotransferase system of Escherichia coli
    Norman D Meadow
    Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    J Biol Chem 281:11450-5. 2006
    ..The results support the hypothesis (Wang, G., Peterkofsky, A., and Clore, G. M. (2000) J. Biol. Chem. 275, 39811-39814) that the N-terminal 18-residue domain "docks" IIAGlc to the lipid bilayer of membranes containing IICBGlc...
  50. ncbi Novel phosphotransferase-encoding genes revealed by analysis of the Escherichia coli genome: a chimeric gene encoding an Enzyme I homologue that possesses a putative sensory transduction domain
    J Reizer
    Department of Biology, University of California at San Diego, La Jolla 92093 0116, USA
    Gene 181:103-8. 1996
    ..It may thereby provide a link between carbon and nitrogen assimilatory pathways...
  51. pmc Spatial and temporal organization of the E. coli PTS components
    Livnat Lopian
    Department of Microbiology and Molecular Genetics, IMRIC, The Hebrew University Faculty of Medicine, Jerusalem, Israel
    EMBO J 29:3630-45. 2010
    ..Hence, the PTS components appear to control bgl operon expression by ushering BglG between the cellular compartments. Our results reinforce the notion that signal transduction in bacteria involves dynamic localization of proteins...
  52. ncbi Membrane localization itself but not binding to IICB is directly responsible for the inactivation of the global repressor Mlc in Escherichia coli
    Yuya Tanaka
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Mol Microbiol 53:941-51. 2004
    ..We conclude that the IICBGlc-Mlc interaction is dispensable for the inactivation of Mlc, and that membrane localization is directly responsible for the inactivation of Mlc...
  53. ncbi Growth-dependent phosphorylation of the PtsN (EIINtr) protein of Pseudomonas putida
    Katharina Pflüger
    Centro Nacional de Biotecnología Consejo Superior de Investigaciones Científicas, Campus de Cantoblanco, Madrid 28049, Spain
    J Biol Chem 282:18206-11. 2007
    ..The large variations of non-phosphorylated PtsN in different growth conditions, in contrast to the systematic excess of the phosphorylated PtsN form, suggested that the P-free PtsN is the predominant signaling species of the protein...
  54. ncbi Effect of the FruR regulator on transcription of the pts operon in Escherichia coli
    S Ryu
    Laboratory of Molecular Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892 4255
    J Biol Chem 270:2489-96. 1995
    ..These results suggest that FruR alone does not mediate the in vivo glucose effect on pts operon expression...
  55. ncbi Potassium mediates Escherichia coli enzyme IIA(Ntr) -dependent regulation of sigma factor selectivity
    Chang Ro Lee
    Department of Biophysics and Chemical Biology, Seoul National University, Seoul 151 742, Republic of Korea
    Mol Microbiol 78:1468-83. 2010
    ..Taken together, the data suggest that EIIA(Ntr) controls sigma factor selectivity by regulating the intracellular K(+) level...
  56. ncbi Non-disruptive release of Pseudomonas putida proteins by in situ electric breakdown of intact cells
    Katharina Pflüger
    Centro Nacional de Biotecnologia CSIC, Campus de Cantoblanco, 28049 Madrid, Spain
    J Microbiol Methods 71:179-85. 2007
    ..The method is applicable to other bacteria as well...
  57. pmc Glucose transport in Escherichia coli mutant strains with defects in sugar transport systems
    Sonja Steinsiek
    MPI für Dynamik Komplexer Technischer Systeme, Magdeburg, Germany
    J Bacteriol 194:5897-908. 2012
    ..Although the galactose transporters are often regarded as important for glucose uptake at micromolar concentrations, the glucose as well as mannose PTS might be sufficient for growth at this relatively low dilution rate...
  58. ncbi Microbial removal of acetate selectively from sugar mixtures
    Arun Lakshmanaswamy
    Center for Molecular BioEngineering, Department of Biological and Agricultural Engineering, University of Georgia, Athens, GA 30602, USA
    J Ind Microbiol Biotechnol 38:1477-84. 2011
    ..coli. These results demonstrate the feasibility of using a substrate-selective approach for the pre-treatment of biomass hydrolysate for microbial processes...
  59. pmc Characteristic phenotypes associated with ptsN-null mutants in Escherichia coli K-12 are absent in strains with functional ilvG
    Marshall Louis Reaves
    Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA
    J Bacteriol 193:4576-81. 2011
    ..We conclude that the ptsN gene product does not regulate organic nitrogen source utilization as previously proposed...
  60. pmc Regulation of the lic operon of Bacillus subtilis and characterization of potential phosphorylation sites of the LicR regulator protein by site-directed mutagenesis
    S Tobisch
    Institut fur Mikrobiologie und Molekularbiologie, Ernst Moritz Arndt Universitat Greifswald, D 17487 Greifswald, Germany
    J Bacteriol 181:4995-5003. 1999
    ..Moreover, the lic operon is subject to carbon catabolite repression (CCR). CCR takes place via a CcpA-dependent mechanism and a CcpA-independent mechanism in which the general PTS enzyme HPr is involved...
  61. ncbi PRD--a protein domain involved in PTS-dependent induction and carbon catabolite repression of catabolic operons in bacteria
    J Stülke
    Lehrstuhl fur Mikrobiologie, Institut fur Mikrobiologie, Biochimie und Genetik der Friedrich Alexander Universität Erlangen Nürnberg, Erlangen, Germany
    Mol Microbiol 28:865-74. 1998
    ..All regulators of this family contain two copies of PRD, which are functionally specialized in either induction or catabolite repression...
  62. ncbi Involvement of a novel transcriptional activator and small RNA in post-transcriptional regulation of the glucose phosphoenolpyruvate phosphotransferase system
    Carin K Vanderpool
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Microbiol 54:1076-89. 2004
    ..Decreased ptsG mRNA results in decreased production of glucose transport machinery, thus limiting further accumulation of glucose phosphate...
  63. pmc Common mechanisms of target cell recognition and immunity for class II bacteriocins
    Dzung B Diep
    Laboratory of Microbial Gene Technology, Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Science, N 1432 As, Norway
    Proc Natl Acad Sci U S A 104:2384-9. 2007
    ....
  64. ncbi The major PEP-phosphotransferase systems (PTSs) for glucose, mannose and cellobiose of Listeria monocytogenes, and their significance for extra- and intracellular growth
    Regina Stoll
    Lehrstuhl fur Mikrobiologie, Biozentrum, Universitat Wurzburg, D 97074 Wurzburg, Germany
    Microbiology 156:1069-83. 2010
    ....
  65. pmc The catabolite repressor/activator (Cra) protein of enteric bacteria
    M H Saier
    Department of Biology, University of California at San Diego, La Jolla, California 92093 0116, USA
    J Bacteriol 178:3411-7. 1996
  66. ncbi Accumulation of glucose 6-phosphate or fructose 6-phosphate is responsible for destabilization of glucose transporter mRNA in Escherichia coli
    Teppei Morita
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    J Biol Chem 278:15608-14. 2003
    ..We conclude that the RNase E-dependent destabilization of ptsG mRNA occurs in response to accumulation of glucose-6-P or fructose-6-P...
  67. ncbi Cell-surface alterations in class IIa bacteriocin-resistant Listeria monocytogenes strains
    Viveka Vadyvaloo
    Department of Biochemistry, University of Stellenbosch, Private Bag X1, 7602 Matieland, South Africa
    Microbiology 150:3025-33. 2004
    ..The authors' findings strongly indicate that all these factors could contribute to class IIa bacteriocin resistance and that the combination and contribution of each of these factors determine the level of bacteriocin resistance...
  68. ncbi Inducer exclusion in Escherichia coli by non-PTS substrates: the role of the PEP to pyruvate ratio in determining the phosphorylation state of enzyme IIAGlc
    B M Hogema
    E C Slater Institute, BioCentrum, University of Amsterdam, Plantage Muidergracht 12, 1018 TV Amsterdam, The Netherlands
    Mol Microbiol 30:487-98. 1998
    ..The implications of these new findings for our view on catabolite repression and inducer exclusion are discussed...
  69. ncbi Corynebacterium diphtheriae: a PTS view to the genome
    S Parche
    , , Germany
    J Mol Microbiol Biotechnol 3:415-22. 2001
    ..The possible significance of these observations in carbon metabolism and the novel features of the detected genes are discussed...
  70. ncbi Understanding glucose transport by the bacterial phosphoenolpyruvate:glycose phosphotransferase system on the basis of kinetic measurements in vitro
    J M Rohwer
    Department of Biochemistry, University of Stellenbosch, Private Bag X1, 7602 Matieland, South Africa jrmaties sun ac za
    J Biol Chem 275:34909-21. 2000
    ....
  71. pmc Characterization of the ccpA gene of Enterococcus faecalis: identification of starvation-inducible proteins regulated by ccpA
    C Leboeuf
    Unité de Microbiologie de l Environnement, Unité soutenue par l INRA, IRBA, Universite de Caen, 14032 Caen Cedex, France
    J Bacteriol 182:5799-806. 2000
    ..The N-terminal amino acid sequences of four of them match sequences deduced from genes coding for L-serine dehydratase, dihydroxyacetone kinase (two genes), and a protein of unknown function from Deinococcus radiodurans...
  72. ncbi Involvement of the mpo operon in resistance to class IIa bacteriocins in Listeria monocytogenes
    Safia Arous
    MFA, Laboratoire de Chimie de l Ean et de l Environnement, UMR CNRA 6008, Universite de Poitiers, 40 Avenue du Recteur Pineau, 86022 Poitiers Cedex, France
    FEMS Microbiol Lett 238:37-41. 2004
    ..We show a cross-regulation between mpo and mpt. In particular, the mpo mutant has a defect in mpt expression that possibly could explain its intermediate resistance phenotype...
  73. ncbi Global control of sugar metabolism: a gram-positive solution
    Fritz Titgemeyer
    Lehrstuhl fur Mikrobiologie, Friedrich Alexander Universitat Erlangen Nurnberg, Staudtstr 5, 91058 Erlangen, Germany
    Antonie Van Leeuwenhoek 82:59-71. 2002
    ..This review summarises the current understanding of carbon catabolite regulation in low-GC Gram-positive bacteria with special emphasis on lactic acid bacteria...
  74. ncbi Use of catabolite repression mutants for fermentation of sugar mixtures to ethanol
    N N Nichols
    National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, Peoria, IL 61604, USA
    Appl Microbiol Biotechnol 56:120-5. 2001
    ..Based upon fermentations of sugar mixtures, a catabolite-repression mutant of ethanologenic E. coli is expected to provide more efficient fermentation of hemicellulose hydrolysates by allowing direct utilization of pentoses...
  75. pmc Vibrio cholerae phosphoenolpyruvate phosphotransferase system control of carbohydrate transport, biofilm formation, and colonization of the germfree mouse intestine
    Laetitia Houot
    Division of Infectious Disease, Children s Hospital Boston, Boston, Massachusetts 02115, USA
    Infect Immun 78:1482-94. 2010
    ..cholerae through its life cycle of pathogenesis and environmental persistence...
  76. pmc Evidence of in vivo cross talk between the nitrogen-related and fructose-related branches of the carbohydrate phosphotransferase system of Pseudomonas putida
    Katharina Pflüger
    Centro Nacional de Biotecnologia CSIC, Campus UAM Cantoblanco, Madrid 28049, Spain
    J Bacteriol 190:3374-80. 2008
    ..The data reported here thus document an unexpected connection in vivo between the sugar-dependent and sugar-independent PTSs...
  77. pmc Expression of the glucose transporter gene, ptsG, is regulated at the mRNA degradation step in response to glycolytic flux in Escherichia coli
    K Kimata
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    EMBO J 20:3587-95. 2001
    ..This is the first instance in which the glycolytic flux has been shown to affect the expression of a specific gene through mRNA stability...
  78. pmc The phosphoenolpyruvate phosphotransferase system regulates Vibrio cholerae biofilm formation through multiple independent pathways
    Laetitia Houot
    Division of Infectious Disease, Children s Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA
    J Bacteriol 192:3055-67. 2010
    ..The presence of multiple, independent biofilm regulatory circuits in the PTS supports the hypothesis that the PTS and PTS-dependent substrates have a central role in sensing environments suitable for a surface-associated existence...
  79. pmc Phosphoenolpyruvate:carbohydrate phosphotransferase system of bacteria
    P W Postma
    Microbiol Rev 49:232-69. 1985
  80. ncbi The three-dimensional structure of the nitrogen regulatory protein IIANtr from Escherichia coli
    D Bordo
    Laboratory of Biophysical Chemistry, University of Groningen, The Netherlands
    J Mol Biol 279:245-55. 1998
    ..The enzyme displays a novel fold characterized by a central mixed parallel/anti-parallel beta-sheet surrounded by six alpha-helices. The active site His73 is situated in a shallow depression on the protein surface...
  81. pmc Regulation of mannose phosphotransferase system permease and virulence gene expression in Listeria monocytogenes by the EII(t)Man transporter
    Hung Vu-Khac
    Department of Molecular Biology, University of Wyoming, 1000 E University Avenue, Dept 3944, Laramie, WY 82071 3944, USA
    Appl Environ Microbiol 75:6671-8. 2009
    ..The results show that EII(t)(Man) participates to various extents in glucose-mediated CCR of PTS operons and makes a small, albeit significant, contribution to downregulation of virulence gene transcription by glucose in strain EGD-e...
  82. ncbi Control of the expression of the manXYZ operon in Escherichia coli: Mlc is a negative regulator of the mannose PTS
    J Plumbridge
    Institut de Biologie Physico Chimique UPR9073, Paris, France
    Mol Microbiol 27:369-80. 1998
    ..The mlc gene is shown to be allelic with the previously characterized dgsA mutation affecting the mannose phosphoenolpyruvate-dependent phosphotransferase system (PTS)...
  83. pmc The phosphotransferase system formed by PtsP, PtsO, and PtsN proteins controls production of polyhydroxyalkanoates in Pseudomonas putida
    Francisco Velazquez
    Centro Nacional de Biotecnologia CSIC, Campus UAM Cantoblanco, Madrid 28049, Spain
    J Bacteriol 189:4529-33. 2007
    ....
  84. pmc Correlation between growth rates, EIIACrr phosphorylation, and intracellular cyclic AMP levels in Escherichia coli K-12
    Katja Bettenbrock
    MPI für Dynamik Komplexer Technischer Systeme, Sandtorstr 1, 39106 Magdeburg, Germany
    J Bacteriol 189:6891-900. 2007
    ..Below and above this range, these parameters were increasingly uncoupled from the growth rate, which perhaps indicates an increasing role executed by other global control systems, in particular the stringent-relaxed response system...
  85. pmc Identification and characterization of a new beta-glucoside utilization system in Bacillus subtilis
    S Tobisch
    Institut fur Mikrobiologie und Molekularbiologie, Ernst Moritz Arndt Universitat, Greifswald, Germany
    J Bacteriol 179:496-506. 1997
    ..Moreover, the expression of the operon requires the general PTS components and seems to be negatively controlled by the specific lic PTS enzymes...
  86. ncbi Control of the Bacillus subtilis antiterminator protein GlcT by phosphorylation. Elucidation of the phosphorylation chain leading to inactivation of GlcT
    Matthias H Schmalisch
    Lehrstuhl fur Mikrobiologie, Friedrich Alexander Universität Erlangen Nuremberg, Staudtstrasse 5, D 91058 Erlangen, Germany
    J Biol Chem 278:51108-15. 2003
    ..This is the first in vitro demonstration of a direct phosphorylation of an antiterminator of the BglG family by the corresponding PTS permease...
  87. ncbi Analysis of carbon metabolism in Escherichia coli strains with an inactive phosphotransferase system by (13)C labeling and NMR spectroscopy
    S Flores
    Departamento de Microbiologia Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, 62250 Cuernavaca, Mexico
    Metab Eng 4:124-37. 2002
    ..The determined carbon fluxes correlate with biochemical and genetic characterizations...
  88. ncbi Molecular characteristics of phosphoenolpyruvate: mannose phosphotransferase system in Streptococcus bovis
    Narito Asanuma
    Department of Life Science, College of Agriculture, Meiji University, Higashimita, Tama ku, Kawasaki 214 8571, Japan
    Curr Microbiol 49:4-9. 2004
    ..The man operon and manO were constitutively transcribed without being affected by culture conditions, such as the sugar supplied (glucose, galactose, fructose, maltose, lactose, sucrose, or mannose), growth rate, or pH...
  89. pmc Role of ptsO in carbon-mediated inhibition of the Pu promoter belonging to the pWW0 Pseudomonas putida plasmid
    I Cases
    , Madrid 28049, Spain
    J Bacteriol 183:5128-33. 2001
    ..These results indicate that some PTS proteins have regulatory functions in P. putida that are independent of their recognized role in sugar transport in other bacteria...
  90. pmc X-ray structure of HPr kinase: a bacterial protein kinase with a P-loop nucleotide-binding domain
    S Fieulaine
    Laboratoire d Enzymologie et Biochimie Structurales, UPR 9063, CNRS, 91198 Gif sur Yvette, France
    EMBO J 20:3917-27. 2001
    ..The results confirm that HprK/P represents a new family of protein kinases, first identified in bacteria, but which may also have members in eukaryotes...
  91. ncbi Expression of galP and glk in a Escherichia coli PTS mutant restores glucose transport and increases glycolytic flux to fermentation products
    Verónica Hernández-Montalvo
    Departamento de Microbiologia Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, UNAM AP 510 3, Cuernavaca, Morelos 62271, Mexico
    Biotechnol Bioeng 83:687-94. 2003
    ....
  92. ncbi Analysis of sigma(54)-dependent genes in Enterococcus faecalis: a mannose PTS permease (EII(Man)) is involved in sensitivity to a bacteriocin, mesentericin Y105
    Y Hechard
    Laboratoire de Microbiologie Fondamentale et Appliquée, CNRS FRE 2224, IBMIG, UFR Sciences, 40 Avenue du Recteur Pineau, 86022 Poitiers Cedex, France
    Microbiology 147:1575-80. 2001
    ..faecalis surface. Finally, it is hypothesized that MptD could be responsible for the targeting specificity, via an interaction between its additional domain and mesentericin Y105...
  93. ncbi A novel beta-glucoside-specific PTS locus from Streptococcus mutans that is not inhibited by glucose
    C K Cote
    Department of Medical Microbiology and Immunology, University of South Florida College of Medicine, 12901 Bruce B Downs Boulevard, Tampa, FL 33612, USA
    Microbiology 146:1555-63. 2000
    ..When a plasmid containing the 8 kb beta-glucoside-specific regulon was transformed into E. coli CC118, the transformed strain was able to break down the beta-glucoside arbutin...
  94. ncbi The ptsI gene encoding enzyme I of the phosphotransferase system of Corynebacterium glutamicum
    P Kotrba
    Research Institute of Innovative Technology for the Earth, 9 2, Kizugawadai, Kizu cho, Soraku gun, Kyoto, 619 0292, Japan
    Biochem Biophys Res Commun 289:1307-13. 2001
    ..Cellobiose was only transported and utilized in adaptive mutants of C. glutamicum R. Cellobiose transport was also found to be PTS-dependent and repressed by PTS sugar glucose...
  95. ncbi HPr kinase/phosphorylase, a Walker motif A-containing bifunctional sensor enzyme controlling catabolite repression in Gram-positive bacteria
    Sandrine Poncet
    Microbiologie et Genetique Moleculaire, CNRS INRA INA PG UMR2585, F 78850 Thiverval Grignon, France
    Biochim Biophys Acta 1697:123-35. 2004
    ..The structures of the complexes of HprK/P with HPr and P-Ser-HPr have also been determined, which allowed proposing a detailed mechanism for the kinase and phosphorylase functions of HprK/P...
  96. pmc In vitro studies with purified components reveal signal recognition particle (SRP) and SecA/SecB as constituents of two independent protein-targeting pathways of Escherichia coli
    H G Koch
    Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, D 79104 Freiburg, Germany
    Mol Biol Cell 10:2163-73. 1999
    ..coli. Both targeting pathways intersect at the translocation pore because they are equally affected by a blocked translocation channel...
  97. ncbi Fructose phosphotransferase system of Xanthomonas campestris pv. campestris: characterization of the fruB gene
    V de Crecy-Lagard
    Unité de Régulation de l Expression Génétique, Institut Pasteur, Paris, France
    Microbiology 141:2253-60. 1995
    ..The three domains are separated by two flexible hinge regions rich in proline and alanine residues. The construction of a fruB mutant confirmed the role of the MTP in fructose transport and phosphorylation in X. campestris...
  98. pmc Escherichia coli enzyme IIANtr regulates the K+ transporter TrkA
    Chang Ro Lee
    Laboratory of Macromolecular Interactions, Department of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 151 742, Korea
    Proc Natl Acad Sci U S A 104:4124-9. 2007
    ..Dephosphorylated EIIA(Glc) regulates a variety of transport systems for carbon sources, whereas dephosphorylated EIIA(Ntr) regulates the transport system for K(+), which has global effects related to nitrogen metabolism...
  99. ncbi Novel proteins of the phosphotransferase system encoded within the rpoN operon of Escherichia coli. Enzyme IIANtr affects growth on organic nitrogen and the conditional lethality of an erats mutant
    B S Powell
    Laboratory of Chromosome Biology, NCI Frederick Cancer Research and Development Center, Maryland 21702 1201
    J Biol Chem 270:4822-39. 1995
    ..These results support our previous proposal of a novel mechanism linking carbon and nitrogen assimilation and relates IIANtr to the unknown process regulated by the essential GTPase Era...
  100. ncbi Binding of the catabolite repressor protein CcpA to its DNA target is regulated by phosphorylation of its corepressor HPr
    B E Jones
    University of Washington, Department of Biochemistry and Biomolecular Structure Center, Seattle, Washington 98195 7742, USA
    J Biol Chem 272:26530-5. 1997
    ..This allows CcpA to recognize the phosphorylation state of HPr, effectively linking the process of sugar import via the PTS to catabolite repression in bacilli...
  101. pmc New beta-glucoside (bgl) genes in Bacillus subtilis: the bglP gene product has both transport and regulatory functions similar to those of BglF, its Escherichia coli homolog
    D Le Coq
    Laboratoire de génétique des microorganismes, Centre National de La Recherche Scientifique URA 537, Institut National Agronomique Paris Grignon, Thiverval Grignon, France
    J Bacteriol 177:1527-35. 1995
    ..Other beta-glucoside genes homologous to bglP and bglH have been recently described in B. subtilis. Thus, B. subtilis possesses several sets of beta-glucoside genes, like E. coli, but these genes do not appear to be cryptic...

Research Grants15

  1. MECHANISM OF CARBOHYDRATE TRANSPORT IN BACTERIA
    Milton Saier; Fiscal Year: 2000
    ..coli and other enteric bacteria that will be of use in the control of these pathogens and will be universally applicable to an understanding of regulatory phenomena in all living cells. ..
  2. ADAPTIVE ACID TOLERANCE OF STREPTOCOCCUS SOBRINUS
    JOSE LEMOS; Fiscal Year: 2007
    ..sobrinus, and (ii) analysis of the acid tolerance properties of a S. sobrinus strain lacking the glucose-specific enzyme II (EIIGIc) of the PTS. [unreadable] [unreadable] [unreadable]..
  3. PHOSPHOENOLPYRUVATE: GLYCOSE PHOSPHOTRANSFERASE SYSTEM
    Saul Roseman; Fiscal Year: 2004
    ..If successful, these experiments should lead toward a molecular explanation for sugar uptake and its regulation by many pathogens. ..
  4. ATP-DEPENDENT PROTEIN KINASES IN GRAM-POSITIVE BACTERIA
    Milton Saier; Fiscal Year: 2004
    ..abstract_text> ..
  5. Glucose Signaling: Regulation of Enzyme Activity
    DONALD PETTIGREW; Fiscal Year: 2006
    ..coli. ..
  6. LOCAL AND LONG RANGE INTERACTIONS IN PROTEIN FOLDING
    J Scholtz; Fiscal Year: 2004
    ..abstract_text> ..
  7. Structural studies on the Initiator protein, IBP39.
    Maria Schumacher; Fiscal Year: 2008
    ..vaginalis suggests it is a possible target for the treatment of trichomoniasis, which afflicts over 170 million individuals each _,ear, ..
  8. Structure/function analysis of the E. coli NIR/NRII signal transduction system
    Alexander Ninfa; Fiscal Year: 2009
    ..Our studies with a model system should allow rapid progress. ..
  9. Structural mechanism of DNA segregation by the pSK41 par system
    Maria Schumacher; Fiscal Year: 2010
    ..Thus, understanding the structural basis for DNA segregation by this par system will provide several points of potential therapeutic intervention against pSK41 harboring multidrug resistant S. aureus strains. ..
  10. INTERACTIONS OF THE SIGNAL TRANSDUCTION PROTEIN GLNK
    Alexander Ninfa; Fiscal Year: 2002
    ..These experiments should elucidate the design of the signal transduction system controlling nitrogen assimilation, and the mechanisms of interaction of PII and GlnK with their receptors and small molecule effectors. ..
  11. Genetic Systems Bioengineering for Escherichia coli
    Alexander Ninfa; Fiscal Year: 2009
    ..In addition we propose designs for novel genetic clocks that test the role of various system design principles in the production of biological oscillations. ..
  12. New statistical tools for single molecule experiments
    David S Talaga; Fiscal Year: 2010
    ..Aim 3 provides useable tools for experimental design and analysis to allow other investigators to exploit these methods for their own research. ..
  13. Structural studies on the P1 plasmid partition apparatus
    Maria Schumacher; Fiscal Year: 2009
    ....