macromolecular substances

Summary

Summary: Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kD in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.

Top Publications

  1. ncbi RING domains: master builders of molecular scaffolds?
    K L Borden
    Department of Physiology and Biophysics, Mt Sinai School of Medicine, New York, NY, 10029, USA
    J Mol Biol 295:1103-12. 2000
  2. ncbi Integrating diverse data for structure determination of macromolecular assemblies
    Frank Alber
    Department of Biopharmaceutical Sciences, and California Institute for Quantitative Biosciences, University of California at San Francisco, CA 94158 2330, USA
    Annu Rev Biochem 77:443-77. 2008
  3. ncbi Macromolecular crowding: obvious but underappreciated
    R J Ellis
    Dept of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK
    Trends Biochem Sci 26:597-604. 2001
  4. pmc Iterative model building, structure refinement and density modification with the PHENIX AutoBuild wizard
    Thomas C Terwilliger
    Los Alamos National Laboratory, Mailstop M888, Los Alamos, NM 87545, USA
    Acta Crystallogr D Biol Crystallogr 64:61-9. 2008
  5. ncbi Optimal determination of particle orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy
    Peter B Rosenthal
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    J Mol Biol 333:721-45. 2003
  6. ncbi Functional organization of the yeast proteome by systematic analysis of protein complexes
    Anne Claude Gavin
    Cellzome AG, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nature 415:141-7. 2002
  7. pmc EMDataBank.org: unified data resource for CryoEM
    Catherine L Lawson
    Department of Chemistry and Chemical Biology and Research Collaboratory for Structural Bioinformatics, Rutgers, The State University of New Jersey, 610 Taylor Road Piscataway, NJ 08854, USA
    Nucleic Acids Res 39:D456-64. 2011
  8. ncbi Macromolecular crowding and protein stability
    Yaqiang Wang
    Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Am Chem Soc 134:16614-8. 2012
  9. ncbi Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution
    R B Sutton
    The Howard Hughes Medical Institute, Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Nature 395:347-53. 1998
  10. ncbi Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry
    Yuen Ho
    MDS Proteomics, 251 Attwell Drive, Toronto, Canada M9W 7H4, and Staermosegaardsvej 6, DK 5230 Odense M, Denmark
    Nature 415:180-3. 2002

Detail Information

Publications385 found, 100 shown here

  1. ncbi RING domains: master builders of molecular scaffolds?
    K L Borden
    Department of Physiology and Biophysics, Mt Sinai School of Medicine, New York, NY, 10029, USA
    J Mol Biol 295:1103-12. 2000
    ..Here, new developments, in the context of previous results, are discussed in an attempt to establish a unifying theory for RING function...
  2. ncbi Integrating diverse data for structure determination of macromolecular assemblies
    Frank Alber
    Department of Biopharmaceutical Sciences, and California Institute for Quantitative Biosciences, University of California at San Francisco, CA 94158 2330, USA
    Annu Rev Biochem 77:443-77. 2008
    ..With these tools, we are poised to integrate structural information gathered at multiple levels of the biological hierarchy--from atoms to cells--into a common framework...
  3. ncbi Macromolecular crowding: obvious but underappreciated
    R J Ellis
    Dept of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK
    Trends Biochem Sci 26:597-604. 2001
    ..Positive results of crowding include enhancing the collapse of polypeptide chains into functional proteins, the assembly of oligomeric structures and the efficiency of action of some molecular chaperones and metabolic pathways...
  4. pmc Iterative model building, structure refinement and density modification with the PHENIX AutoBuild wizard
    Thomas C Terwilliger
    Los Alamos National Laboratory, Mailstop M888, Los Alamos, NM 87545, USA
    Acta Crystallogr D Biol Crystallogr 64:61-9. 2008
    ..1 to 3.2 A, resulting in a mean R factor of 0.24 and a mean free R factor of 0.29. The R factor of the final model is dependent on the quality of the starting electron density and is relatively independent of resolution...
  5. ncbi Optimal determination of particle orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy
    Peter B Rosenthal
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    J Mol Biol 333:721-45. 2003
    ..7A resolution, according to a new objective criterion for resolution assessment proposed here...
  6. ncbi Functional organization of the yeast proteome by systematic analysis of protein complexes
    Anne Claude Gavin
    Cellzome AG, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nature 415:141-7. 2002
    ..This higher-order map contains fundamental biological information and offers the context for a more reasoned and informed approach to drug discovery...
  7. pmc EMDataBank.org: unified data resource for CryoEM
    Catherine L Lawson
    Department of Chemistry and Chemical Biology and Research Collaboratory for Structural Bioinformatics, Rutgers, The State University of New Jersey, 610 Taylor Road Piscataway, NJ 08854, USA
    Nucleic Acids Res 39:D456-64. 2011
    ....
  8. ncbi Macromolecular crowding and protein stability
    Yaqiang Wang
    Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Am Chem Soc 134:16614-8. 2012
    ..Our results show that both chemical interactions and hard-core repulsions must be considered when assessing the effects of crowding and help explain previous observations about protein stability and dynamics in cells...
  9. ncbi Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution
    R B Sutton
    The Howard Hughes Medical Institute, Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Nature 395:347-53. 1998
    ..These characteristics may be important for membrane fusion and for the binding of regulatory factors affecting neurotransmission...
  10. ncbi Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry
    Yuen Ho
    MDS Proteomics, 251 Attwell Drive, Toronto, Canada M9W 7H4, and Staermosegaardsvej 6, DK 5230 Odense M, Denmark
    Nature 415:180-3. 2002
    ..Given the high degree of connectivity observed in this study, even partial HMS-PCI coverage of complex proteomes, including that of humans, should allow comprehensive identification of cellular networks...
  11. ncbi Crystal structure of Arp2/3 complex
    R C Robinson
    Structural Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 294:1679-84. 2001
    ..ARPC3 p21 and ARPC5 p16 are globular alpha-helical subunits. We predict that WASp/Scar proteins activate Arp2/3 complex by bringing Arp2 into proximity with Arp3 for nucleation of a branch on the side of a preexisting actin filament...
  12. ncbi ZDOCK: an initial-stage protein-docking algorithm
    Rong Chen
    Bioinformatics Program, Boston University, Boston, Massachusetts, USA
    Proteins 52:80-7. 2003
    ..All four scoring functions have been integrated into our Fast Fourier Transform based docking algorithm ZDOCK, which is freely available to academic users at //zlab.bu.edu/~ rong/dock...
  13. pmc MOLPROBITY: structure validation and all-atom contact analysis for nucleic acids and their complexes
    Ian W Davis
    Department of Biochemistry, Duke University, Durham, NC 27710 3711, USA
    Nucleic Acids Res 32:W615-9. 2004
    ..This service is available free to all users at //kinemage.biochem.duke.edu...
  14. ncbi Vesicle tethering complexes in membrane traffic
    James R C Whyte
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    J Cell Sci 115:2627-37. 2002
    ..Moreover, the diversity of tethering complexes in the cell suggests that not all tethering events occur through the same mechanisms...
  15. ncbi Cell biology: join the crowd
    R John Ellis
    Nature 425:27-8. 2003
  16. ncbi Macromolecular crowding: qualitative and semiquantitative successes, quantitative challenges
    Damien Hall
    Section on Physical Biochemistry, Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD 20892 0830, USA
    Biochim Biophys Acta 1649:127-39. 2003
    ..Recently suggested novel approaches to quantitative analysis of crowding phenomena, which may help to overcome some of the limitations of current theory, are summarized...
  17. ncbi Structure of the cell-puncturing device of bacteriophage T4
    Shuji Kanamaru
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907 1392, USA
    Nature 415:553-7. 2002
    ..The amino-terminal, antiparallel beta-barrel domain of gp5 is inserted into a cylinder formed by three gp27 monomers, which may serve as a channel for DNA ejection...
  18. pmc Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin
    K A Knudsen
    Lankenau Medical Research Center, Wynnewood, Pennsylvania 19096, USA
    J Cell Biol 130:67-77. 1995
    ..We therefore propose that cadherin/catenin complexes are linked to the actin cytoskeleton via a direct association between alpha-actinin and alpha-catenin...
  19. ncbi Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body
    Markus Babst
    Department of Cellular and Molecular Medicine and Howard Hughes Medical Institute, School of Medicine, University of California, San Diego, La Jolla 92093, USA
    Dev Cell 3:283-9. 2002
    ..We propose that the ESCRT complexes perform a coordinated cascade of events to select and sort MVB cargoes for delivery to the lumen of the vacuole/lysosome...
  20. ncbi RIM1alpha forms a protein scaffold for regulating neurotransmitter release at the active zone
    Susanne Schoch
    The Center for Basic Neuroscience and Department of Molecular Genetics, Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center, Dallas, Texas 75390 9111, USA
    Nature 415:321-6. 2002
    ..These data indicate that RIM1alpha has a central function in integrating active zone proteins and synaptic vesicles into a molecular scaffold that controls neurotransmitter release...
  21. ncbi Mammalian tumor susceptibility gene 101 (TSG101) and the yeast homologue, Vps23p, both function in late endosomal trafficking
    M Babst
    Division of Cellular and Molecular Medicine and Howard Hughes Medical Institute, School of Medicine, University of California at San Diego, La Jolla, CA 92093 0668, USA
    Traffic 1:248-58. 2000
    ..This may contribute to the tumorigenic phenotype exhibited by the tsg101 mutant fibroblasts...
  22. ncbi Situs: A package for docking crystal structures into low-resolution maps from electron microscopy
    W Wriggers
    Department of Pharmacology, University of California, 9500 Gilman Drive, San Diego, California 92093 0365, USA
    J Struct Biol 125:185-95. 1999
    ..The precision of the docking has allowed for the extraction of unique conformations of the macromolecules and is limited only by the reliability of the underlying structural data...
  23. ncbi How can biochemical reactions within cells differ from those in test tubes?
    Allen P Minton
    Section on Physical Biochemistry, Laboratory of Biochemical Pharmacology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health U S DHHS, Bethesda, MD 20892, USA
    J Cell Sci 119:2863-9. 2006
    ..Greater than order-of-magnitude increases in association rate and equilibrium constants attributable to background interactions have been observed in simulated and actual intracellular environments...
  24. pmc Crowding and hydrodynamic interactions likely dominate in vivo macromolecular motion
    Tadashi Ando
    Center for the Study of Systems Biology, School of Biology, Georgia Institute of Technology, 250 14th Street North West, Atlanta, GA 30318 5304, USA
    Proc Natl Acad Sci U S A 107:18457-62. 2010
    ..These qualitative differences provide a testable means of differentiating the importance of HI vs. nonspecific attractive interactions on macromolecular motion in cells...
  25. pmc The Vps4p AAA ATPase regulates membrane association of a Vps protein complex required for normal endosome function
    M Babst
    Division of Cellular Molecular Medicine and Howard Hughes Medical Institute, University of California at San Diego, School of Medicine La Jolla, CA 92093 0668, USA
    EMBO J 17:2982-93. 1998
    ..Together, the data suggest that the Vps4 ATPase catalyzes the release (uncoating) of an endosomal membrane-associated class E protein complex(es) required for normal morphology and sorting activity of the endosome...
  26. ncbi 3-D structures of macromolecules using single-particle analysis in EMAN
    Steven J Ludtke
    Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX, USA
    Methods Mol Biol 673:157-73. 2010
    ..EMAN is one of the easier to master software suites for single-particle analysis. This protocol explains how to perform an initial low-resolution reconstruction using EMAN...
  27. pmc The analysis of macromolecular interactions by sedimentation equilibrium
    Rodolfo Ghirlando
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0540, USA
    Methods 54:145-56. 2011
    ....
  28. ncbi PyTom: a python-based toolbox for localization of macromolecules in cryo-electron tomograms and subtomogram analysis
    Thomas Hrabe
    Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    J Struct Biol 178:177-88. 2012
    ..PyTom enables parallelized processing of large numbers of tomograms, but also provides a convenient, sustainable environment for algorithmic development...
  29. ncbi Macromolecular connections of active zone material to docked synaptic vesicles and presynaptic membrane at neuromuscular junctions of mouse
    Sharuna Nagwaney
    Department of Neurobiology, Stanford University School of Medicine, Stanford, California 94305, USA
    J Comp Neurol 513:457-68. 2009
    ..These secondary docked vesicles had a different relationship to the membrane and AZM macromolecules than primary docked vesicles, consistent with their having a different AZM-regulated behavior...
  30. pmc Overexpression of the dynamitin (p50) subunit of the dynactin complex disrupts dynein-dependent maintenance of membrane organelle distribution
    J K Burkhardt
    The University of Chicago, Department of Pathology, Chicago, Illinois 60637, USA
    J Cell Biol 139:469-84. 1997
    ..These results suggest that dynamitin plays a pivotal role in regulating organelle movement at the level of motor-cargo binding...
  31. ncbi Classification of cryo-electron sub-tomograms using constrained correlation
    Friedrich Förster
    Max Planck Institut fur Biochemie, Molekulare Strukturbiologie, Am Klopferspitz 18, 82152 Martinsried, Germany
    J Struct Biol 161:276-86. 2008
    ..Moreover, we apply our classification method to an experimental dataset of GroEL with and without GroES, where we achieve a distinct discrimination between the putative GroEL and GroEL/GroES complexes...
  32. ncbi Polyglutamine protein aggregates are dynamic
    Soojin Kim
    Department of Biochemistry, Molecular Biology and Cell Biology, Rice Institute for Biomedical Research, Northwestern University, Evanston, IL 60208, USA
    Nat Cell Biol 4:826-31. 2002
    ..These studies provide new insights into the composite organization and formation of protein aggregates and show that molecular chaperones are not sequestered into aggregates, but are instead transiently associated...
  33. ncbi Macromolecular therapeutics: advantages and prospects with special emphasis on solid tumour targeting
    Khaled Greish
    Department of Microbiology, Kumamoto University School of Medicine, Kumamoto, Japan
    Clin Pharmacokinet 42:1089-105. 2003
    ..This article discusses these and other polymeric drugs in the setting of targeting to solid tumours...
  34. ncbi Extending recognition by peptide nucleic acids (PNAs): binding to duplex DNA and inhibition of transcription by tail-clamp PNA-peptide conjugates
    Kunihiro Kaihatsu
    Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390 9041, USA
    Biochemistry 42:13996-4003. 2003
    ..and Nielsen, P. E. (2003) Biochemistry 42, 13987-13995], expand the range of sequences within duplex DNA that are accessible to PNAs and suggest that TC-PNA-peptide conjugates are good candidates for further testing as antigene agents...
  35. ncbi Tumor-selective delivery of macromolecular drugs via the EPR effect: background and future prospects
    Hiroshi Maeda
    Laboratory of Microbiology and Oncology, Faculty of Pharmaceutical Sciences, Division of Applied Chemistry, Graduate School of Engineering, Sojo University, Kumamoto, 860 0082, Japan
    Bioconjug Chem 21:797-802. 2010
    ..These data suggest very appealing prospects for utilization of the EPR effect in future development of cancer therapeutics...
  36. ncbi Analysis of inter-alpha-trypsin inhibitor and a novel trypsin inhibitor, pre-alpha-trypsin inhibitor, from human plasma. Polypeptide chain stoichiometry and assembly by glycan
    J J Enghild
    Pathology Department, Duke University Medical Center, Durham, North Carolina 27710
    J Biol Chem 264:15975-81. 1989
    ..Our data allow identification of several recently reported cDNA clones and clarify the confusion surrounding the composition of plasma proteins referred to as inter-alpha-trypsin inhibitor...
  37. ncbi C. elegans PAT-4/ILK functions as an adaptor protein within integrin adhesion complexes
    A Craig Mackinnon
    Department of Cell and Structural Biology, University of Illinois, Urbana Champaign, 601 South Goodwin Avenue, Urbana, IL 61801, USA
    Curr Biol 12:787-97. 2002
    ..ILK has been implicated in integrin-mediated signaling and is also an adaptor within integrin-associated cytoskeletal complexes...
  38. pmc Conserved stem II of the box C/D motif is essential for nucleolar localization and is required, along with the 15.5K protein, for the hierarchical assembly of the box C/D snoRNP
    Nicholas J Watkins
    Max Planck Institut fur biophysikalische Chemie, Abteilung Zelluläre Biochemie, D 37070, Gottingen, Germany
    Mol Cell Biol 22:8342-52. 2002
    ..5K, to the snoRNA. Indeed, the sequence of stem II was essential for nucleolar localization of U14 snoRNA microinjected into HeLa cells. Thus, the conserved sequence of stem II determines the specific assembly of the box C/D snoRNP...
  39. ncbi A multiubiquitin chain is confined to specific lysine in a targeted short-lived protein
    V Chau
    Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201
    Science 243:1576-83. 1989
    ..The experiments with ubiquitin mutated at its Lys48 residue indicate that the multiubiquitin chain in a targeted protein is essential for the degradation of the protein...
  40. ncbi Crystal structure of the B7-1/CTLA-4 complex that inhibits human immune responses
    C C Stamper
    Departments of Biological Chemistry and Musculoskeletal Sciences, Wyeth Research, 87 Cambridge Park Drive, Cambridge, Massachusetts 02140, USA
    Nature 410:608-11. 2001
    ..This zipper-like oligomerization provides the structural basis for forming unusually stable signalling complexes at the T-cell surface, underscoring the importance of potent inhibitory signalling in human immune responses...
  41. ncbi Designing supramolecular protein assemblies
    Todd O Yeates
    UCLA Department of Chemistry and Biochemistry, 611Charles E Young Drive East, University of California, Los Angeles, 90095 1569, USA
    Curr Opin Struct Biol 12:464-70. 2002
    ..Recent advances include the design of novel proteins that assemble into filaments, symmetric cages and regular arrays...
  42. ncbi The Partner of Inscuteable/Discs-large complex is required to establish planar polarity during asymmetric cell division in Drosophila
    Y Bellaiche
    Ecole Normale Superieure, UMR 8544, 46 rue d Ulm, 75230 Paris Cedex 05, France
    Cell 106:355-66. 2001
    ..Finally, Baz and the Dlg/Pins complex are required for the asymmetric localization of Numb. Thus, the Dlg/Pins complex responds to Fz signaling to establish planar asymmetry in the pI cell...
  43. pmc Characterization of interactions between the mammalian polycomb-group proteins Enx1/EZH2 and EED suggests the existence of different mammalian polycomb-group protein complexes
    R G Sewalt
    E C Slater Instituut, BioCentrum Amsterdam, University of Amsterdam, 1018 TV Amsterdam, The Netherlands
    Mol Cell Biol 18:3586-95. 1998
    ..Our findings indicate that Enx1/EZH2 and EED are members of a class of PcG proteins that is distinct from previously described human PcG proteins...
  44. pmc The KEGG databases at GenomeNet
    Minoru Kanehisa
    Bioinformatics Center, Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Nucleic Acids Res 30:42-6. 2002
    ..The KEGG databases are updated daily and made freely available (//www.genome.ad.jp/kegg/)...
  45. pmc Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex
    T Guan
    Department of Cell and Molecular Biology, Scripps Research Institute, La Jolla, California 92037, USA
    Mol Biol Cell 6:1591-603. 1995
    ..The properties and localization of the p62 complex suggest that it may be involved in binding transport ligands near the center of the nuclear pore complex and in subsequently transferring them to the gated transport channel...
  46. ncbi Normal mode analysis with simplified models to investigate the global dynamics of biological systems
    Florence Tama
    Department of Molecular Biology TPC6, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Protein Pept Lett 10:119-32. 2003
    ..In this review, theoretical approaches to the study of dynamics of biological systems and their application are discussed. In particular, simplified models for the normal mode analysis are described...
  47. pmc Saccharomyces cerevisiae peroxisomal thiolase is imported as a dimer
    J R Glover
    Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada
    Proc Natl Acad Sci U S A 91:10541-5. 1994
    ..This observation suggests that interactions between thiolase subunits are not disrupted during translocation...
  48. ncbi Structure of the 30S ribosomal subunit
    B T Wimberly
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Nature 407:327-39. 2000
    ..The structure will facilitate the interpretation in molecular terms of lower resolution structural data on several functional states of the ribosome from electron microscopy and crystallography...
  49. pmc Recruitment, activation and retention of caspases-9 and -3 by Apaf-1 apoptosome and associated XIAP complexes
    S B Bratton
    Medical Research Council Toxicology Unit, Hodgkin Building, University of Leicester, PO Box 138, Lancaster Road, Leicester LE1 9HN, UK
    EMBO J 20:998-1009. 2001
    ..Thus, XIAP may regulate cell death by inhibiting the activation of caspase-3 within the apoptosome and by preventing release of active caspase-3 from the complex...
  50. ncbi The Mre11 complex is required for repair of hairpin-capped double-strand breaks and prevention of chromosome rearrangements
    Kirill S Lobachev
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Cell 108:183-93. 2002
    ..Our results suggest an additional role for the Mre11 complex in maintaining genome stability...
  51. ncbi Characterization of Saccharomyces cerevisiae Nop17p, a novel Nop58p-interacting protein that is involved in Pre-rRNA processing
    Fernando A Gonzales
    Department of Biochemistry, Chemistry Institute, USP, Sao Paulo, SP, Brazil
    J Mol Biol 346:437-55. 2005
    ....
  52. ncbi Identification of the spectrin subunit and domains required for formation of spectrin/adducin/actin complexes
    X Li
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 271:15695-702. 1996
    ....
  53. ncbi The role of presenilin cofactors in the gamma-secretase complex
    Nobumasa Takasugi
    Department of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences, University of Tokyo, 7 3 1 Hongo, Bunkyoku, Tokyo 113 0033, Japan
    Nature 422:438-41. 2003
    ..Thus, APH-1 stabilizes the presenilin holoprotein in the complex, whereas PEN-2 is required for endoproteolytic processing of presenilin and conferring gamma-secretase activity to the complex...
  54. ncbi Inferential structure determination
    Wolfgang Rieping
    Unite de Bioinformatique Structurale, Institut Pasteur, CNRS URA 2185, 25 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France
    Science 309:303-6. 2005
    ..We implemented this approach by using Markov chain Monte Carlo techniques. Our method provides an objective figure of merit and improves structural quality...
  55. ncbi Phosphorylation of the Cdc42 exchange factor Cdc24 by the PAK-like kinase Cla4 may regulate polarized growth in yeast
    M P Gulli
    Swiss Institute for Experimental Cancer Research Chemin des Boveresses 155 1066 Epalinges, Vaud, Switzerland
    Mol Cell 6:1155-67. 2000
    ....
  56. pmc The Pfam protein families database
    Alex Bateman
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nucleic Acids Res 30:276-80. 2002
    ..New search tools, including taxonomy search and domain query, greatly add to the functionality and usability of the Pfam resource...
  57. ncbi GIT1 functions in a motile, multi-molecular signaling complex that regulates protrusive activity and cell migration
    Ri ichiroh Manabe
    Department of Cell Biology, University of Virginia, Charlottesville VA 22908, USA
    J Cell Sci 115:1497-510. 2002
    ..Thus, GIT1 is a component of a motile, multimolecular complex that traffics a set of signaling components to specific locations in the cell where they regulate localized activities...
  58. pmc Septin ring assembly involves cycles of GTP loading and hydrolysis by Cdc42p
    Amy S Gladfelter
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    J Cell Biol 156:315-26. 2002
    ....
  59. pmc The p150Glued component of the dynactin complex binds to both microtubules and the actin-related protein centractin (Arp-1)
    C M Waterman-Storer
    Department of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia 19104 6046
    Proc Natl Acad Sci U S A 92:1634-8. 1995
    ..These results demonstrate that p150Glued, a protein implicated in cytoplasmic dynein-based microtubule motility, is capable of direct binding to both microtubules and centractin...
  60. ncbi Wide varieties of cationic nanoparticles induce defects in supported lipid bilayers
    Pascale R Leroueil
    Department of Chemistry, Michigan Nanotechnology Institute for Medicine and Biological Sciences, University of Michigan, Ann Arbor, Michigan 48019, USA
    Nano Lett 8:420-4. 2008
    ....
  61. pmc CDC55, a Saccharomyces cerevisiae gene involved in cellular morphogenesis: identification, characterization, and homology to the B subunit of mammalian type 2A protein phosphatase
    A M Healy
    Department of Biology, University of Michigan, Ann Arbor 48109 1048
    Mol Cell Biol 11:5767-80. 1991
    ..An extragenic suppressor of the cdc55 mutation lies in BEM2, a gene previously identified on the basis of an apparent role in bud emergence...
  62. ncbi Mutation of a gene that encodes a kinesin-like protein blocks nuclear division in A. nidulans
    A P Enos
    Department of Pharmacology, UMDNJ Robert Wood Johnson Medical School, Piscataway 08854
    Cell 60:1019-27. 1990
    ..We have cloned and sequenced the wild-type bimC gene. The predicted protein product has homology to Drosophila kinesin heavy chain. We conclude that this kinesin-like protein has an important role in nuclear division in Aspergillus...
  63. ncbi Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein
    T Hirano
    Cold Spring Harbor Laboratory, New York 11724, USA
    Cell 89:511-21. 1997
    ..Our results suggest that the condensin complexes represent the most abundant structural components of mitotic chromosomes and play a central role in driving chromosome condensation...
  64. ncbi Small GTP-binding proteins and the regulation of the actin cytoskeleton
    A Hall
    MRC Laboratory for Molecular Cell Biology, University College London, England
    Annu Rev Cell Biol 10:31-54. 1994
  65. pmc The mechanism of topoisomerase I poisoning by a camptothecin analog
    Bart L Staker
    deCODE Genetics, Incorporated, BioStructures Group, 7869 Northeast Day Road West, Bainbridge Island, WA 98110, USA
    Proc Natl Acad Sci U S A 99:15387-92. 2002
    ..The first class includes changes to residues that contribute to direct interactions with the drug, whereas a second class would alter interactions with the DNA and thereby destabilize the drug-binding site...
  66. pmc Insights into peptide nucleic acid (PNA) structural features: the crystal structure of a D-lysine-based chiral PNA-DNA duplex
    Valeria Menchise
    Istituto di Biostrutture e Bioimmagini, Consiglio Nazionale delle Ricerche, Via Mezzocannone 6, I 80134 Naples, Italy
    Proc Natl Acad Sci U S A 100:12021-6. 2003
    ..The conformational rigidity of PNAs is enhanced by the presence of the chiral centers, limiting the ability of PNA strands to adopt other conformations and, ultimately, increasing the selectivity in molecular recognition...
  67. pmc A dynamin-cortactin-Arp2/3 complex mediates actin reorganization in growth factor-stimulated cells
    Eugene W Krueger
    Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota 55905, USA
    Mol Biol Cell 14:1085-96. 2003
    ....
  68. pmc Increased bending rigidity of single DNA molecules by H-NS, a temperature and osmolarity sensor
    Roee Amit
    Department of Physics of Complex Systems, The Weizmann Institute of Science, Rehovot 76100, Israel
    Biophys J 84:2467-73. 2003
    ..Thus, we propose that H-NS plays a unique structural role, different from that of HU and IHF, and functions as one of the environmental sensors of the cell...
  69. pmc The WD repeat protein, Mdv1p, functions as a molecular adaptor by interacting with Dnm1p and Fis1p during mitochondrial fission
    Quinton Tieu
    Section of Molecular and Cellular Biology, University of California, Davis, 95616, USA
    J Cell Biol 158:445-52. 2002
    ....
  70. pmc The multiprotein exocyst complex is essential for cell separation in Schizosaccharomyces pombe
    Hongyan Wang
    The Institute of Molecular Agrobiology, The National University of Singapore, Singapore 117604, Republic of Singapore
    Mol Biol Cell 13:515-29. 2002
    ..We further propose that cell elongation and division septum assembly can continue with minimal levels of exocyst function...
  71. pmc Sedimentation velocity analysis of heterogeneous protein-protein interactions: Lamm equation modeling and sedimentation coefficient distributions c(s)
    Julie Dam
    Center for Advanced Research in Biotechnology, W M Keck Laboratory for Structural Biology, University of Maryland Biotechnology Institute, Rockville, Maryland, USA
    Biophys J 89:619-34. 2005
    ..The integration of the c(s) peaks can lead to isotherms of species populations or s-value of the reaction boundary, respectively, which can be used for the determination of the equilibrium binding constant...
  72. ncbi The crystal structure of the estrogen receptor DNA-binding domain bound to DNA: how receptors discriminate between their response elements
    J W Schwabe
    Medical Research Council Laboratory of Molecular Biology, Cambridge, England
    Cell 75:567-78. 1993
    ..Since all nuclear hormone receptors recognize one or the other of these two consensus half site sequences, this recognition mechanism applies generally to the whole receptor family...
  73. ncbi DM enhances peptide binding to class II MHC by release of invariant chain-derived peptide
    M A Sherman
    Department of Pathology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Immunity 3:197-205. 1995
    ..We conclude that DM is a cofactor that enhances peptide binding to DR molecules through a mechanism involving peptide exchange...
  74. ncbi Mechanism of tumor-targeted delivery of macromolecular drugs, including the EPR effect in solid tumor and clinical overview of the prototype polymeric drug SMANCS
    H Maeda
    Department of Microbiology, Kumamoto University School of Medicine, 860 0811, Kumamoto, Japan
    J Control Release 74:47-61. 2001
    ..Last, SMANCS/Lipiodol is discussed with reference to the type of formulation for arterial injection with most pronounced tumor selective delivery, as well as its advantages, precautions, and side effects from the clinical standpoint...
  75. ncbi Near-field-magnetic-tweezer manipulation of single DNA molecules
    Jie Yan
    Department of Physics, University of Illinois at Chicago, 845 West Taylor Street, Chicago, Illinois 60607 7059, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 70:011905. 2004
    ..We demonstrate the use of our system to study formation of DNA loops by an enzyme which strongly binds two copies of a specific 6-base-pair sequence...
  76. ncbi Structure and properties of nano-hydroxyapatite/polymer composite scaffolds for bone tissue engineering
    Guobao Wei
    Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109 2009, USA
    Biomaterials 25:4749-57. 2004
    ..The results suggest that the newly developed NHAP/polymer composite scaffolds may serve as an excellent 3D substrate for cell attachment and migration in bone tissue engineering...
  77. ncbi Assembly of the cadherin-catenin complex in vitro with recombinant proteins
    H Aberle
    Max Planck Institut fur Immunbiologie, Freiburg, Germany
    J Cell Sci 107:3655-63. 1994
    ..The reconstitution in vitro of the cadherin-catenin complex should allow the study of the interaction with signalling molecules and with the actin-based cytoskeleton...
  78. ncbi Three-dimensional structure of the neuronal-Sec1-syntaxin 1a complex
    K M Misura
    Department of Structural Biology, Stanford University School of Medicine, California 94305, USA
    Nature 404:355-62. 2000
    ..The structure also indicates mechanisms that might couple the action of upstream effector proteins to conformational changes in syntaxin 1a and nSec1 that lead to core complex formation and membrane fusion...
  79. ncbi Exit from mitosis is triggered by Tem1-dependent release of the protein phosphatase Cdc14 from nucleolar RENT complex
    W Shou
    Division of Biology, California Institute of Technology, Pasadena, California 91125, USA
    Cell 97:233-44. 1999
    ..Nucleolar sequestration may be a general mechanism for the regulation of diverse biological processes...
  80. pmc Fluorescence resonance energy transfer over approximately 130 basepairs in hyperstable lac repressor-DNA loops
    Laurence M Edelman
    Department of Chemistry and Biochemistry, University of Maryland, College Park, College Park, Maryland 20742 2021, USA
    Biophys J 84:1131-45. 2003
    ..LacI flexibility may allow it to maintain looping with other proteins bound or under different intracellular conditions...
  81. pmc Guanidine hydrochloride inhibits the generation of prion "seeds" but not prion protein aggregation in yeast
    Frederique Ness
    Department of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, United Kingdom
    Mol Cell Biol 22:5593-605. 2002
    ....
  82. ncbi Reconstitution of gamma-secretase activity
    Dieter Edbauer
    Adolf Butenandt Institute, Department of Biochemistry, Laboratory for Alzheimer s and Parkinson s Disease Research, Ludwig Maximilians University, 80336 Munich, Germany
    Nat Cell Biol 5:486-8. 2003
    ..Thus, the biological activity of gamma-secretase is reconstituted by the co-expression of human PS, Nct, APH-1 and PEN-2 in yeast...
  83. pmc Cryo-EM of macromolecular assemblies at near-atomic resolution
    Matthew L Baker
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, USA
    Nat Protoc 5:1697-708. 2010
    ..Additionally, the feature recognition and computational modeling tools can be applied to any near-atomic resolution density maps, including those from X-ray crystallography...
  84. pmc An Agrobacterium VirB10 mutation conferring a type IV secretion system gating defect
    Lois M Banta
    Department of Biology, Williams College, Williamstown, MA 01267, USA
    J Bacteriol 193:2566-74. 2011
    ..We propose that (i) G272R disrupts a gating mechanism in the core chamber that regulates substrate passage across the OM and (ii) the G272R and ΔAP mutations block pilus production at distinct steps of the pilus biogenesis pathway...
  85. ncbi A LIS1/NUDEL/cytoplasmic dynein heavy chain complex in the developing and adult nervous system
    S Sasaki
    Shirakawa Institute of Animal Genetics, Odakura Nishigo Nishishirakawa, 961 8061, Fukushima, Japan
    Neuron 28:681-96. 2000
    ..These results suggest that LIS1 and NUDEL regulate CDHC activity during neuronal migration and axonal retrograde transport in a Cdk5/p35-dependent fashion...
  86. ncbi Comprehensive proteomic analysis of the human spliceosome
    Zhaolan Zhou
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 419:182-5. 2002
    ..This complexity may be required not only for splicing multi-intronic metazoan pre-messenger RNAs, but also for mediating the extensive coupling between splicing and other steps in gene expression...
  87. ncbi Ral GTPases regulate exocyst assembly through dual subunit interactions
    Serge Moskalenko
    Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75235 9039, USA
    J Biol Chem 278:51743-8. 2003
    ....
  88. ncbi New electron microscopy database and deposition system
    Mohamed Tagari
    European Bioinformatics Institute, EMBL Outstation Hinxton, Wellcome Trust Genome Campus, Cambridge, UK CB10 1SD
    Trends Biochem Sci 27:589. 2002
    ..To manage, organize and disseminate data on the structure of biological macromolecules solved by 3D electron microscopy, an electron microscopy database has been set up at the European Bioinformatics Institute...
  89. ncbi A two-dimensional spectrum analysis for sedimentation velocity experiments of mixtures with heterogeneity in molecular weight and shape
    Emre Brookes
    Department of Biochemistry, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, MC 7760, San Antonio, TX 78229 3901, USA
    Eur Biophys J 39:405-14. 2010
    ....
  90. ncbi Determining the architectures of macromolecular assemblies
    Frank Alber
    Department of Bioengineering and Therapeutic Sciences, and California Institute for Quantitative Biosciences, Byers Hall, Suite 503B, 1700 4th Street, University of California at San Francisco, San Francisco, California 94158 2330, USA
    Nature 450:683-94. 2007
    ..The present approach should be applicable to many other macromolecular assemblies...
  91. ncbi Effects of macromolecular crowding on burst phase kinetics of cytochrome c folding
    Eefei Chen
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    Biochemistry 51:9836-45. 2012
    ..We conclude that excluded volume effects alter unfolded cytochrome c such that access to fast-folding conformations is reduced...
  92. ncbi A recyclable supramolecular membrane for size-selective separation of nanoparticles
    Elisha Krieg
    Department of Organic Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
    Nat Nanotechnol 6:141-6. 2011
    ..Unlike conventional membranes, our supramolecular membranes can be disassembled using organic solvent, cleaned, reassembled and reused multiple times...
  93. pmc Collagen IV alpha 3, alpha 4, and alpha 5 chains in rodent basal laminae: sequence, distribution, association with laminins, and developmental switches
    J H Miner
    Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, Missouri 63110
    J Cell Biol 127:879-91. 1994
    ..Thus, collagen alpha 1 and alpha 2(IV) and laminin B1 appear to be fetal components of the glomerular BL, and there is a developmental switch to collagen alpha 3-alpha 5(IV) and S-laminin expression...
  94. ncbi Applying macromolecular crowding to enhance extracellular matrix deposition and its remodeling in vitro for tissue engineering and cell-based therapies
    Clarice Chen
    Division of Bioengineering, Faculty of Engineering, National University of Singapore, Singapore
    Adv Drug Deliv Rev 63:277-90. 2011
    ..We begin to understand how well cells really can perform ex vivo if given the chance...
  95. pmc Uvomorulin-catenin complex formation is regulated by a specific domain in the cytoplasmic region of the cell adhesion molecule
    M Ozawa
    Max Planck Institut fur Immunbiologie, Forschergruppe Molekulare Embryologie, Stübeweg, Freiburg, Federal Republic of Germany
    Proc Natl Acad Sci U S A 87:4246-50. 1990
    ..From these results we conclude that the cytoplasmic anchorage of uvomorulin is of major biological importance...
  96. ncbi Cooperativity in macromolecular assembly
    James R Williamson
    Department of Molecular Biology, Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Chem Biol 4:458-65. 2008
    ..A significant challenge remains in tackling the thermodynamics and kinetics of the intermolecular interactions required for all cellular functions...
  97. ncbi Cytosolic delivery of membrane-impermeable molecules in dendritic cells using pH-responsive core-shell nanoparticles
    Yuhua Hu
    Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Nano Lett 7:3056-64. 2007
    ....
  98. pmc Macromolecular docking restrained by a small angle X-ray scattering profile
    Dina Schneidman-Duhovny
    Department of Bioengineering and Therapeutic Sciences, and California Institute for Quantitative Biosciences QB3, University of California at San Francisco, CA 94158, USA
    J Struct Biol 173:461-71. 2011
    ..Thus, the integrative approach significantly improves on molecular docking alone. The improvement arises from an increased resolution of rigid docking sampling and more accurate scoring...
  99. ncbi Cadherin-directed actin assembly: E-cadherin physically associates with the Arp2/3 complex to direct actin assembly in nascent adhesive contacts
    Eva M Kovacs
    Department of Physiology and Pharmacology, School of Biomedical Science, 4072, Brisbane, Australia
    Curr Biol 12:379-82. 2002
    ....
  100. ncbi Regulation of signaling protein function and trafficking by the hsp90/hsp70-based chaperone machinery
    William B Pratt
    Department of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0632, USA
    Exp Biol Med (Maywood) 228:111-33. 2003
    ..This purified system should facilitate understanding of how eukaryotic hsp70 and hsp90 work together as essential components of a process that alters the conformations of substrate proteins to states that respond in signal transduction...
  101. ncbi X-ray structure of a protein-conducting channel
    Bert van den Berg
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 427:36-44. 2004
    ....

Research Grants69

  1. METAL DEPENDENT COMPLEXES IN BLOOD COAGULATION
    Barbara Furie; Fiscal Year: 2002
    ..A long-term goal of these studies is to determine, by X-ray crystallography, the structure of factor Va heavy chain-prothrombin fragment 1-calcium complex. ..
  2. Novel TIRF microscopy analyzing trafficking & signaling at the cell cortex
    Derek Toomre; Fiscal Year: 2009
    ..Consistent with the recovery act's goal, this funding will provide full-time summer employment for 4 undergraduate students and accelerate scientific achievement of the parent DP2 award...
  3. T cell regulation by N-glycosylation
    Michael Demetriou; Fiscal Year: 2013
    ..Here we further explore the mechanisms by which protein glycosylation regulates immune function and autoimmunity. ..
  4. A Systems Biology Approach to the Model Apicomplexan Toxoplasma gondii
    William J Sullivan Jr; Fiscal Year: 2010
    ..Gathering these data will be important for developing new treatments that will prevent T. gondii from persisting in infected people, and understanding how Apicomplexan parasites cause disease. ..
  5. Proteomics of RNA polymerase interactomes in pathogenic bacteria
    Evgeny Nudler; Fiscal Year: 2013
    ..abstract_text> ..
  6. Cellular/molecular Na,K-ATPase regulation in choroid plexus
    Kathleen J Sweadner; Fiscal Year: 2010
    ....
  7. Designing an Integrated Nanoscale System for Ion Channel Structure-Function Study
    Ratneshwar Lal; Fiscal Year: 2013
    ....
  8. RNA Dynamics of Transient Macromolecular Complexes in Cancer Cells
    Jack D Keene; Fiscal Year: 2013
    ....
  9. MICROFILAMENTS IN THE YEAST SACCHAROMYCES CEREVISIAE
    Anthony P Bretscher; Fiscal Year: 2013
    ....
  10. Regulation of Axon Guidance by Second Messengers
    Timothy M Gomez; Fiscal Year: 2013
    ..As a number of cognitive disorders result from improper axon pathfinding, understanding the molecular basis for normal neural development is essential for designing therapeutic interventions. ..
  11. Reading the histone code:nanoscale morphology of Epigneomic Histone Modifications
    M Mitchell Smith; Fiscal Year: 2010
    ....
  12. Human Autoimmunity and Genetic Defects in N-Glycosylation
    Michael Demetriou; Fiscal Year: 2013
    ..Metabolically supplementing the pathway with a simple sugar suppresses immune hyperactivity and development of autoimmunity, suggesting human disease may be treated/prevented with metabolic therapy. ..
  13. RPE Lactate Transporters: A Role in Retinal Survival
    Nancy Philp; Fiscal Year: 2009
    ..Additionally, these studies will further our understanding of how changes in expression and activity of MCTs contribute to pathophysiological changes found in aging and diseased eyes. ..
  14. RPE Lactate Transporters: A Role in Retinal Survival
    NANCY JEAN PHILP; Fiscal Year: 2012
    ..abstract_text> ..
  15. Red Cell Deformability InVitro and Survival InVivo
    MOHANDAS NARLA; Fiscal Year: 2013
    ..It is anticipated that the findings from the proposed studies will contribute to better clinical management of red cell disorders and for developing new therapeutic approaches for management of hemolytic anemias. ..
  16. REPLICATION OF INFLUENZA VIRUS
    Robert M Krug; Fiscal Year: 2013
    ..These results should be relevant to mechanisms by which IFN-induced ISG15 and its conjugation affect infection by other viruses. ..
  17. Chaperone Assisted Crystallography
    Anthony A Kossiakoff; Fiscal Year: 2012
    ....
  18. Molecular regulation of breast cancer growth
    Marsha A Moses; Fiscal Year: 2010
    ....
  19. Control of Macromolecular Traffic Through Plasmodesmata
    VITALY H CITOVSKY; Fiscal Year: 2013
    ..abstract_text> ..
  20. AvanceIII 700MHz NMR spectrometer with cryogenic probe
    James J Chou; Fiscal Year: 2010
    ..The proposed 700 MHz NMR spectrometer would provide us sufficient instrumentation to move the above studies forward, while maintaining the postdoctoral fellow positions involved in these projects;it has no environmental impact. ..
  21. Novel Pattern Specific Isotopic Labeling of Aromatic Amino Acids
    Robert McFeeters; Fiscal Year: 2010
    ..To demonstrate the utility of the new reagents and to establish guidelines for their implementation, the researchers will apply the newly developed, inexpensive reagents to multiple recombinant systems. ..
  22. Multiple Sclerosis and Genetic Defects in N-Glycosylation
    Michael Demetriou; Fiscal Year: 2009
    ..Specific Aim 3 will associate genetic variants identified in Specific Aim 1 and/or 2 in large Multiple Sclerosis co-horts. ..
  23. Structural Studies of Bacterial Transcription Initiation
    Brian Bae; Fiscal Year: 2013
    ..thermophilus CarD will be conducted. The results of these studies will be combined with in vivo studies of M. tuberculosis CarD (from collaborators) to provide a comprehensive picture of CarD in vitro mechanism and in vivo function. ..
  24. CHC22 CLATHRIN FUNCTION IN HUMAN GLUCOSE METABOLISM
    FRANCES BRODSKY; Fiscal Year: 2009
    ..The research proposed here will establish novel characteristics of human glucose homeostasis and will enable development of better animal models for studying its dysfunction in diabetes. ..
  25. Regulation of Microtubule Motors
    Vladimir I Gelfand; Fiscal Year: 2013
    ..ii) To find contribution of proteins that are not associated with microtubules to transport regulation (iii) To use neuronal model to find how microtubules and microtubule-dependent transport generate formation of cell processes. ..
  26. Computer Integrated Systems for Microscopy and Manipulation
    Richard Superfine; Fiscal Year: 2013
    ..abstract_text> ..
  27. Computational modeling of viral assembly: encapsulation of nucleic acids and env
    Michael F Hagan; Fiscal Year: 2013
    ..A new method to apply Markov state models to assembly reactions is developed. ..
  28. Using ChAP-MS to Study Macromolecular Chromatin Composition during Transcription
    Sean D Taverna; Fiscal Year: 2013
    ....
  29. Molecular Mechanisms of Mammalian SIRT6 Function
    Katrin F Chua; Fiscal Year: 2010
    ..In addition, genome wide approaches will be employed to identify SIRT6-regulated genes and SIRT6 binding sites at chromatin. ..
  30. Structure determination of reaction intermediates in macromolecular complexes
    Amie K Boal; Fiscal Year: 2013
    ..abstract_text> ..
  31. Structure determination of reaction intermediates in macromolecular complexes
    Amie K Boal; Fiscal Year: 2013
    ....
  32. FLUCTUATIONS IN IONIC CURRENT THROUGH MEMBRANE CHANNELS
    FREDERICK J SIGWORTH; Fiscal Year: 2012
    ..2, one of the best-studied voltage-gated potassium channels. By creating membrane potentials in the vesicles, we will be able to trap this channel in its closed as well as open states for structure determination. ..
  33. Structural Studies of Ion Channel Assembly and Signaling Complexes
    Daniel L Minor; Fiscal Year: 2013
    ..Our work aims to understand the molecular architecture that underlies VGIC function. Such understanding has direct relevance for development of more efficacious treatments of nervous system and cardiovascular disorders. ..
  34. New Tools for Exploring the Dynamic Interactome
    Michael P Rout; Fiscal Year: 2011
    ..abstract_text> ..
  35. Regulation of RNA Polymerase II by Non-coding RNAs
    James Goodrich; Fiscal Year: 2013
    ....
  36. The molecular basis for high density lipoprotein heterogeneity
    W Sean Davidson; Fiscal Year: 2011
    ..This knowledge may be useful for developing predictive biomarkers and for developing preventative treatments for cardiovascular disease. ..
  37. Targeted therapy for 11q23 acute leukemias
    Charles S Hemenway; Fiscal Year: 2012
    ..These pre-clinical studies are intended to lay the groundwork for pharmaceutical drug development. ..
  38. Lipid droplets as protein sequestration sites
    MICHAEL ANDREAS WELTE; Fiscal Year: 2013
    ..Most importantly, these studies will provide a paradigm for how and why proteins from other compartments are transiently sequestered on lipid droplets. ..
  39. An Alternative Strategy for Macromolecular Crystallization
    Alexander McPherson; Fiscal Year: 2011
    ..The strategy proposed here is essentially orthogonal to current approaches and has an objective of doubling the success rate of today. ..
  40. Potassium Channels as Macromolecular Complexes
    Thomas A Neubert; Fiscal Year: 2013
    ..Our findings may have important implications for understanding the role of KATP channels in the heart and coronary vasculature under physiological and non-pathophysiological conditions. ..
  41. Software for Homology Modeling of Ribosomes
    FREDRICK OTIENO SIJENYI; Fiscal Year: 2010
    ..RNA-123 will enable scientists with the latest, most accurate bioinformatics tool available, having an immediate impact on all of humanity. ..
  42. Structure-Function Studies of the Modular Human Mediator Coactivator Complex
    Robert G Roeder; Fiscal Year: 2010
    ....
  43. Regulation and Maintenance of Cardiac Muscle Sarcomere Integrity
    Chee Chew Lim; Fiscal Year: 2013
    ..The proposed experimental aims will allow us to evaluate and refine this model, and advance our understanding of the complex physiological and temporal aspects of myofilament sarcomere turnover. ..
  44. The Macromolecular Organization of Leukotriene Synthesis and Renal Inflammation
    ANGELA BAIR SCHMIDER; Fiscal Year: 2013
    ..The broad, long-term objective of this proposal is to determine how the assembly of LT membrane synthetic complexes is regulated and to identify each of the components and their roles. ..
  45. Macromolecular Diffraction Resource: MacCHESS
    Richard A Cerione; Fiscal Year: 2013
    ..abstract_text> ..
  46. KV CHANNEL FUNCTIONING IN MACROMOLECULAR COMPLEXES
    Jeanne M Nerbonne; Fiscal Year: 2010
    ....
  47. Crystallography without crystals: Atomic structure determination of laser oriente
    Wei Kong; Fiscal Year: 2010
    ..With tremendous savings in human effort, time, and money, daring hypotheses on disease mechanisms and radical therapeutic strategies could be tested from structural information in a timely and cost-effective manner. ..
  48. A Large-area Pixel Array Detector System for Macromolecular Crystallography
    William I Weis; Fiscal Year: 2010
    ..It will maintain international competitiveness for NIH funded researchers and will contribute to retaining staff and maintaining jobs. ..
  49. Accurate Modeling in Structural Genomics
    Michael Levitt; Fiscal Year: 2013
    ..This set of structures then provides a statistical sanity check, showing how much better the best model is than the next best one. ..
  50. CryoEM analysis of Anthrax Toxin Pore Complexes
    Mark T Fisher; Fiscal Year: 2013
    ....
  51. JOINT CENTER FOR STRUCTURAL GENOMICS
    Ian A Wilson; Fiscal Year: 2013
    ..Furthermore, we will strive along with the PSI Network to promote widespread use of the PSI resources, materials, methodologies, data and models to the general scientific community. ..
  52. Technologies to Define and Map Novel Interorganelle Macromolecular Interactions
    VLADISLAV VERKHUSHA; Fiscal Year: 2013
    ..The combined expertise from these investigators gives us a unique opportunity to discover novel organelles and macromolecular complexes involved in interorganelle contacts, and define their cell biology. ..
  53. STUDIES ON MICROTUBULE BINDING PROTEINS
    Ronald A Milligan; Fiscal Year: 2013
    ..The structural results we obtain will provide the basis for a mechanistic understanding of some of the most important events taking place on spindle microtubules during cell division. ..
  54. Characterization of the mRNP closed-loop structure
    Clyde L Denis; Fiscal Year: 2013
    ..Finally, the exact translational complexes bound by translational repressors, SBP1, SCD6, and NPL3, will be determined by AU-FDS using Flag-tagged versions of PAB1, eIF4E, and eRF1. ..
  55. Mechanistic Studies of the Hsp70 Chaperone System
    EUGENE GUY MAES; Fiscal Year: 2010
    ..Both mutational and biochemical experiments will be used to investigate the observed intermolecular interactions and mechanistic hypotheses inferred from inspection of these structures. ..
  56. Conformational cycles of molecular chaperones
    Daniel N Bolon; Fiscal Year: 2013
    ..The powerful combination of in vivo experiments and protein engineering together with thermodynamic and kinetic analyses will provide unique insight into the mechanism of Hsp90. ..
  57. ITC Ultrasensitive Microcalorimeter for Measuring Biomolecular Interactions
    LUIS ARNALDO MARKY; Fiscal Year: 2010
    ..Furthermore, it will help in the creation of a Center for Pharmaceutical Biophysics at UNMC, which is a current strategic initiative of the Department of Pharmaceutical Sciences. ..
  58. Dual-Beam Scanning Electron Microscope for New York Structural Biology Center
    David L Stokes; Fiscal Year: 2010
    ..By adding the new technologies provided by dual-beam SEM those already supported at NYSBC, we will significantly enhance its standing as a hub of structural biology in the greater New York metropolitan area. ..
  59. Acquisition for Illumina HiSeq 2500
    George S Grills; Fiscal Year: 2012
    ..The instrument will facilitate a broad range of current and future NIH funded projects at Cornell University. ..
  60. Nuclear export of RNAs by nuclear envelope budding
    VIVIAN G BUDNIK; Fiscal Year: 2013
    ....
  61. Applying peptide and protein domain microarrays to epigenetic research
    Or Gozani; Fiscal Year: 2009
    ..Our proposed studies should provide insight into how epigenetic dynamics functional at the molecular level, and potentially lead to the discovery of new epigenetic targets for therapeutic intervention in diverse human diseases. ..
  62. Integrated Methods for Structural Elucidation of Proteins and Macromolecular Comp
    Evan R Williams; Fiscal Year: 2013
    ..These approaches will be applied to investigate the structures and structural transitions that occur in several complexes where limited information has been obtained, including Anthrax toxin complexes, and the 26S proteasome. ..
  63. Investigation of calcium modulation in cardiomyocytes by novel methods
    Geoffrey S Pitt; Fiscal Year: 2013
    ....
  64. MOLECULAR INTERACTIONS OF BLOOD COAGULATION FACTOR X
    RICHARD PAINTER; Fiscal Year: 1991
    ..Identification of sites of interactions on factor X/Xa may lead to the production of therapeutic agents (peptides and/or monoclonal antibodies) which can impede coagulation and regulate hemostasis...
  65. Structure/Function Study of the Anticoagulant Protein S
    Alan Rigby; Fiscal Year: 2005
    ..New insights into these mechanisms may identify novel methods for the intervention in pathologic thrombus formation. ..
  66. New roles of Src tyrosine kinases in vascular tone
    LIGIA TORO DE STEFANI; Fiscal Year: 2007
    ..The elucidation of primary steps involved in 5-HT and TXA2 smooth muscle stimulation should help in the design of new therapeutic ways to control cardiovascular disease. [unreadable] [unreadable]..
  67. Local Signals and Macromolecular Architecture in Heart
    Terry Rogers; Fiscal Year: 2006
    ..abstract_text> ..
  68. KIDNEY ISCHEMIA AND TIGHT JUNCTION RECOVERY
    Sanjay Nigam; Fiscal Year: 2001
    ..He will then determine total vs. radiolabeled tight junction protein in immunoprecipitates from macromolecular complex, the extractable fraction, and in the cell-surface pool. ..
  69. X-LINKED RETINITIS PIGMENTOSA
    Hemant Khanna; Fiscal Year: 2009
    ..Our investigations should also allow better design of therapies for XLRP and other human diseases involving ciliary dysfunction. ..