sterols

Summary

Summary: Steroids with a hydroxyl group at C-3 and most of the skeleton of cholestane. Additional carbon atoms may be present in the side chain. (IUPAC Steroid Nomenclature, 1987)

Top Publications

  1. pmc Phylogenetic distribution of fungal sterols
    John D Weete
    Auburn Research and Technology Foundation, Auburn, Alabama, United States of America
    PLoS ONE 5:e10899. 2010
  2. ncbi Protein sensors for membrane sterols
    Joseph L Goldstein
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Cell 124:35-46. 2006
  3. ncbi Gp78, a membrane-anchored ubiquitin ligase, associates with Insig-1 and couples sterol-regulated ubiquitination to degradation of HMG CoA reductase
    Bao Liang Song
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Mol Cell 19:829-40. 2005
  4. pmc Identification and characterization of major lipid particle proteins of the yeast Saccharomyces cerevisiae
    K Athenstaedt
    Institut fur Biochemie und Lebensmittelchemie, Technische Universität and SFB Biomembrane Research Center, Petersgasse 12 2, A 8010 Graz, Austria
    J Bacteriol 181:6441-8. 1999
  5. ncbi Combined therapy with cinacalcet and low doses of vitamin D sterols in patients with moderate to severe secondary hyperparathyroidism
    Geoffrey A Block
    Denver Nephrologists, Denver, CO 80230, USA
    Nephrol Dial Transplant 23:2311-8. 2008
  6. pmc Sterol-induced degradation of HMG CoA reductase depends on interplay of two Insigs and two ubiquitin ligases, gp78 and Trc8
    Youngah Jo
    Department of Molecular Genetics and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
    Proc Natl Acad Sci U S A 108:20503-8. 2011
  7. ncbi Structural and biochemical properties of lipid particles from the yeast Saccharomyces cerevisiae
    Tibor Czabany
    Institute of Biochemistry, Graz University of Technology, Petersgasse 12, A 8010 Graz, Austria
    J Biol Chem 283:17065-74. 2008
  8. pmc Lipid particles/droplets of the yeast Saccharomyces cerevisiae revisited: lipidome meets proteome
    Karlheinz Grillitsch
    Austrian Centre of Industrial Biotechnology, Graz University of Technology, Austria
    Biochim Biophys Acta 1811:1165-76. 2011
  9. ncbi Extraction and analysis of sterols in biological matrices by high performance liquid chromatography electrospray ionization mass spectrometry
    Jeffrey G McDonald
    Department of Molecular Genetics, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, USA
    Methods Enzymol 432:145-70. 2007
  10. ncbi Hepatic nuclear sterol regulatory binding element protein 2 abundance is decreased and that of ABCG5 increased in male hamsters fed plant sterols
    Scott V Harding
    Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, Canada R3T 6C5
    J Nutr 140:1249-54. 2010

Detail Information

Publications341 found, 100 shown here

  1. pmc Phylogenetic distribution of fungal sterols
    John D Weete
    Auburn Research and Technology Foundation, Auburn, Alabama, United States of America
    PLoS ONE 5:e10899. 2010
    ..Ergosterol has been considered the "fungal sterol" for almost 125 years; however, additional sterol data superimposed on a recent molecular phylogeny of kingdom Fungi reveals a different and more complex situation...
  2. ncbi Protein sensors for membrane sterols
    Joseph L Goldstein
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Cell 124:35-46. 2006
    ..Here, we discuss recent advances that explain how cells employ an ensemble of membrane-embedded proteins to monitor sterol concentrations and adjust sterol synthesis and uptake...
  3. ncbi Gp78, a membrane-anchored ubiquitin ligase, associates with Insig-1 and couples sterol-regulated ubiquitination to degradation of HMG CoA reductase
    Bao Liang Song
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Mol Cell 19:829-40. 2005
    ..The current results identify gp78 as the E3 that initiates sterol-accelerated degradation of reductase, and Insig-1 as a bridge between gp78/VCP and the reductase substrate...
  4. pmc Identification and characterization of major lipid particle proteins of the yeast Saccharomyces cerevisiae
    K Athenstaedt
    Institut fur Biochemie und Lebensmittelchemie, Technische Universität and SFB Biomembrane Research Center, Petersgasse 12 2, A 8010 Graz, Austria
    J Bacteriol 181:6441-8. 1999
    ..Strains with YBR177c, YMR313c, and YKL140w deleted exhibited phospholipid and/or neutral lipid patterns that were different from the wild-type strain and thus may be further candidate ORFs involved in yeast lipid metabolism...
  5. ncbi Combined therapy with cinacalcet and low doses of vitamin D sterols in patients with moderate to severe secondary hyperparathyroidism
    Geoffrey A Block
    Denver Nephrologists, Denver, CO 80230, USA
    Nephrol Dial Transplant 23:2311-8. 2008
    ..of the current study was to determine whether treatment with cinacalcet combined with low doses of vitamin D sterols improves control of both PTH and Ca x P among haemodialysis patients with secondary hyperparathyroidism (sHPT).
  6. pmc Sterol-induced degradation of HMG CoA reductase depends on interplay of two Insigs and two ubiquitin ligases, gp78 and Trc8
    Youngah Jo
    Department of Molecular Genetics and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
    Proc Natl Acad Sci U S A 108:20503-8. 2011
    Accumulation of sterols in membranes of the endoplasmic reticulum (ER) leads to the accelerated ubiquitination and proteasomal degradation of 3-hydroxy-3-methylglutaryl coenzyme A reductase, a rate-limiting enzyme in synthesis of ..
  7. ncbi Structural and biochemical properties of lipid particles from the yeast Saccharomyces cerevisiae
    Tibor Czabany
    Institute of Biochemistry, Graz University of Technology, Petersgasse 12, A 8010 Graz, Austria
    J Biol Chem 283:17065-74. 2008
    ..LP from triple mutants contained only TAG or SE, respectively, with specific patterns of fatty acids and sterols. Biophysical investigations, however, revealed that individual neutral lipids strongly affected the internal ..
  8. pmc Lipid particles/droplets of the yeast Saccharomyces cerevisiae revisited: lipidome meets proteome
    Karlheinz Grillitsch
    Austrian Centre of Industrial Biotechnology, Graz University of Technology, Austria
    Biochim Biophys Acta 1811:1165-76. 2011
    ..esters (SE) are stored in so-called lipid particles/droplets (LP) as biologically inert form of fatty acids and sterols. To understand LP structure and function in more detail we investigated the molecular equipment of this ..
  9. ncbi Extraction and analysis of sterols in biological matrices by high performance liquid chromatography electrospray ionization mass spectrometry
    Jeffrey G McDonald
    Department of Molecular Genetics, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, USA
    Methods Enzymol 432:145-70. 2007
    ..liquid chromatography mass spectrometry (HPLC-MS) method that allows the identification and quantitation of sterols in mammalian cells and tissues...
  10. ncbi Hepatic nuclear sterol regulatory binding element protein 2 abundance is decreased and that of ABCG5 increased in male hamsters fed plant sterols
    Scott V Harding
    Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, Canada R3T 6C5
    J Nutr 140:1249-54. 2010
    The effect of dietary plant sterols on cholesterol homeostasis has been well characterized in the intestine, but how plant sterols affect lipid metabolism in other lipid-rich tissues is not known...
  11. pmc Reversal of fluconazole resistance by sulfated sterols from the marine sponge Topsentia sp
    Jessica A DiGirolamo
    Department of Pharmacognosy, National Center for Natural Products Research, and Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, Mississippi 38677, USA
    J Nat Prod 72:1524-8. 2009
    Bioassay-guided fractionation of the extract of Topsentia sp. led to the identification of two new sulfated sterols, geodisterol-3-O-sulfite (1) and 29-demethylgeodisterol-3-O-sulfite (2), the active constituents reversing efflux pump-..
  12. pmc Functional interactions between sphingolipids and sterols in biological membranes regulating cell physiology
    Xue Li Guan
    Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117456, Singapore
    Mol Biol Cell 20:2083-95. 2009
    b>Sterols and sphingolipids are limited to eukaryotic cells, and their interaction has been proposed to favor formation of lipid microdomains...
  13. ncbi Oxysterols: modulators of cholesterol metabolism and other processes
    G J Schroepfer
    Departments of Biochemistry, Rice University, Houston, Texas, USA
    Physiol Rev 80:361-554. 2000
    ..Oxysterols are variously metabolized to esters, bile acids, steroid hormones, cholesterol, or other sterols through pathways that may differ according to the type of cell and mode of experimentation (in vitro, in vivo, ..
  14. ncbi Synthesis and turnover of non-polar lipids in yeast
    Sona Rajakumari
    Institute of Biochemistry, Graz University of Technology, Petersgasse 12 2, A 8010 Graz, Austria
    Prog Lipid Res 47:157-71. 2008
    ..Moreover, we will discuss non-polar lipid homeostasis and its newly discovered links to various cell biological processes in the yeast...
  15. ncbi The SREBP pathway in Drosophila: regulation by palmitate, not sterols
    Adam C Seegmiller
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Dev Cell 2:229-38. 2002
    ..Processing requires dSCAP, but is not inhibited by sterols as in mammals. Instead, dSREBP processing is blocked by palmitic acid...
  16. ncbi Sterol regulators of cholesterol homeostasis and beyond: the oxysterol hypothesis revisited and revised
    Saloni Gill
    BABS, School of Biotechnology and Biomolecular Sciences, Biosciences, Building D26, University of New South Wales, Sydney, NSW 2052, Australia
    Prog Lipid Res 47:391-404. 2008
    ..Some (oxy)sterols can accelerate the degradation of the key cholesterol biosynthetic enzyme, HMG-CoA reductase, and/or serve as ..
  17. pmc Segregation of sphingolipids and sterols during formation of secretory vesicles at the trans-Golgi network
    Robin W Klemm
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    J Cell Biol 185:601-12. 2009
    ..How the TGN sorts proteins and lipids to generate the enrichment of sphingolipids and sterols at the plasma membrane is poorly understood...
  18. pmc Decreased NPC1L1 expression in the liver from Chinese female gallstone patients
    Wei Cui
    Department of Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Institute of Digestive Surgery, 200025, Shanghai, PR China
    Lipids Health Dis 9:17. 2010
    ..The molecular mechanisms underlying the relationship between female gallstone disease and hepatic sterol transporters are still undergoing definition and have not been evaluated in humans...
  19. ncbi Oxysterol biosynthetic enzymes
    D W Russell
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75235 9046, USA
    Biochim Biophys Acta 1529:126-35. 2000
    ..and fat metabolism, serve as substrates for the synthesis of bile acids, and are intermediates in the transfer of sterols from the periphery to the liver...
  20. ncbi Metabolism of conjugated sterols in eggplant. Part 1. UDP-glucose : sterol glucosyltransferase
    Anna Potocka
    Institute of Biochemistry, Warsaw University, Warszawa, Poland
    Acta Biochim Pol 55:127-34. 2008
    ..Among a wide spectrum of 3-OH steroids (i.e. typical plant sterols, androstane, pregnane and cholestane derivatives, steroidal alkaloids and sapogenins) and triterpenic alcohols, ..
  21. ncbi Structure and cytotoxicity of new polyhydroxylated sterols from the Caribbean gorgonian Plexaurella grisea
    A Rueda
    , Facultad de Ciencias del Mar, , Apdo. 40, 11510 Puerto Real, , Spain
    Steroids 66:897-904. 2001
    ..All the compounds (1-13) isolated from P. grisea were tested against P 388, A 549, and HT 29 tumor cell lines. Compounds 3, 5/6, and 12 exhibited selective activity against the HT 29 cell line (ED(50) = 0.1 microg/ml)...
  22. ncbi Effects of hippuristanol, an inhibitor of eIF4A, on adult T-cell leukemia
    Tomoyuki Tsumuraya
    Department of Microbiology and Oncology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903 0215, Japan
    Biochem Pharmacol 81:713-22. 2011
    ..These preclinical data suggest that hippuristanol could be a potentially useful therapeutic agent for patients with ATL...
  23. pmc Rate constants for peroxidation of polyunsaturated fatty acids and sterols in solution and in liposomes
    Libin Xu
    Department of Chemistry and Vanderbilt Institute of Chemical Biology, Vanderbilt University, Nashville, Tennessee 37235, USA
    J Am Chem Soc 131:13037-44. 2009
    ..We suggest that peroxyl radical beta-scission depends on solvent polarity and the polarity of the local environment of peroxyl radicals in liposomal oxidations depends on the position of the peroxyl radical on the 18-carbon chain...
  24. ncbi Accelerated degradation of HMG CoA reductase mediated by binding of insig-1 to its sterol-sensing domain
    Navdar Sever
    Department of Molecular Genetics, University of Texas, Southwestern Medical Center, Dallas, TX 75390, USA
    Mol Cell 11:25-33. 2003
    b>Sterols accelerate degradation of the ER enzyme 3-hydroxy-3-methylglutaryl CoA reductase (HMG CoA reductase), which catalyzes a rate-controlling step in cholesterol biosynthesis...
  25. pmc Targeted lipidomic analysis of oxysterols in the embryonic central nervous system
    Yuqin Wang
    Institute of Mass Spectrometry, School of Medicine, Grove Building, Swansea University, Singleton Park, Swansea, UKSA2 8PP
    Mol Biosyst 5:529-41. 2009
    ....
  26. ncbi Sterol-regulated degradation of Insig-1 mediated by the membrane-bound ubiquitin ligase gp78
    Joon No Lee
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 281:39308-15. 2006
    ..studies show that Insig-1 (but not Insig-2) is ubiquitinated and rapidly degraded when cells are depleted of sterols. Conversely, ubiquitination of Insig-1 is blocked, and the protein is stabilized when intracellular sterols ..
  27. pmc Functional convergence of hopanoids and sterols in membrane ordering
    James Peter Sáenz
    Max Plank Institute for Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 109:14236-40. 2012
    ..The formation of a liquid-ordered phase depends crucially on the ordering properties of sterols. However, it is not known whether this capacity exists in organisms that lack sterols, such as bacteria...
  28. pmc Arginine-482 is not essential for transport of antibiotics, primary bile acids and unconjugated sterols by the human breast cancer resistance protein (ABCG2)
    Tavan Janvilisri
    Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK
    Biochem J 385:419-26. 2005
    ..Interestingly, BCRP-R exhibited a previously unestablished ability to transport antibiotics, unconjugated sterols and primary bile acids in L. lactis, for which the R482G mutation was not critical...
  29. ncbi Isolation of mutant cells lacking Insig-1 through selection with SR-12813, an agent that stimulates degradation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase
    Navdar Sever
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9046, USA
    J Biol Chem 279:43136-47. 2004
    ..produced by gamma-irradiation, followed by selection with the 1,1-bisphosphonate ester SR-12813, which mimics sterols in accelerating reductase degradation but does not block SREBP processing...
  30. ncbi Cytotoxic sterols from the soft coral Nephthea erecta
    C Y Duh
    Department of Marine Resources, National Sun Yat Sen University, Kaohsiung, Taiwan, Republic of China
    J Nat Prod 61:1022-4. 1998
    Two new cytotoxic sterols, 24-methylcholesta-5,24(28)-diene-3beta, 15beta,19-triol (1) and 24-methylcholesta-5,24(28)-diene-3beta, 19-diol-7-one (2), as well as four cytotoxic sterols, 24-methylcholesta-5,24(28)-diene-3beta,19-diol (3), ..
  31. ncbi Biosynthesis and accumulation of sterols
    Pierre Benveniste
    Institut de Biologie Moleculaire des Plantes, Departement Biogenese et Fonctions des Isoprenoides, UPR CNRS 2357, 28 rue Goethe, 67083 Strasbourg, France
    Annu Rev Plant Biol 55:429-57. 2004
    ..The greater selectivity of these techniques constitutes an invaluable advantage and has led to the discovery of a role for sterols in plant development.
  32. ncbi The metabolism of plant sterols is disturbed in postmenopausal women with coronary artery disease
    Helena Gylling
    Department of Clinical Nutrition, University of Kuopio, FIN 70211 Kuopio, Finland
    Metabolism 58:401-7. 2009
    In postmenopausal coronary artery disease (CAD) women, serum plant sterols are elevated...
  33. pmc Specificity of cholesterol and analogs to modulate BK channels points to direct sterol-channel protein interactions
    Anna N Bukiya
    Department of Pharmacology, College of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, USA
    J Gen Physiol 137:93-110. 2011
    ..Finally, cholesterol and coprostanol reduced Po, yet these sterols have opposite effects on tight lipid packing and, likely, on lateral stress...
  34. ncbi Insig-dependent ubiquitination and degradation of mammalian 3-hydroxy-3-methylglutaryl-CoA reductase stimulated by sterols and geranylgeraniol
    Navdar Sever
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9046, USA
    J Biol Chem 278:52479-90. 2003
    ..endoplasmic reticulum enzyme 3-hydroxy-3-methylglutaryl-CoA reductase produces mevalonate, which is converted to sterols and to other products, including geranylgeraniol groups attached to proteins...
  35. ncbi 4-Methylenesterols from Theonella swinhoei sponge are natural pregnane-X-receptor agonists and farnesoid-X-receptor antagonists that modulate innate immunity
    Simona De Marino
    Dipartimento di Chimica delle Sostanze Naturali, Universita di Napoli Federico II, via D Montesano 49, 80131 Napoli, Italy
    Steroids 77:484-95. 2012
    ..These results pave the way to development of a dual PXR agonist/FXR antagonist with a robust immunomodulatory activity and endowed with the ability to modulate the expression of bile acid-regulated genes in the liver...
  36. pmc Mechanism of fluconazole resistance in Candida albicans biofilms: phase-specific role of efflux pumps and membrane sterols
    Pranab K Mukherjee
    Center for Medical Mycology, Department of Dermatology, University Hospitals of Cleveland and Case Western Reserve University, Ohio 44106, USA
    Infect Immun 71:4333-40. 2003
    ..001) at intermediate and mature phases, compared to those in early-phase biofilms. These studies suggest that multicomponent, phase-specific mechanisms are operative in antifungal resistance of fungal biofilms...
  37. pmc Survival strategies of a sterol auxotroph
    Maria Carvalho
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Development 137:3675-85. 2010
    ..Drosophila cannot synthesize sterols, but do require them for development...
  38. ncbi Selective sterol accumulation in ABCG5/ABCG8-deficient mice
    Liqing Yu
    McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
    J Lipid Res 45:301-7. 2004
    ..cassette (ABC) transporters ABCG5 and ABCG8 limit intestinal absorption and promote biliary secretion of neutral sterols. Mutations in either gene cause sitosterolemia, a rare recessive disease in which plasma and tissue levels of ..
  39. ncbi Theonellasterols and conicasterols from Theonella swinhoei. Novel marine natural ligands for human nuclear receptors
    Simona De Marino
    Dipartimento di Chimica delle Sostanze Naturali, Università di Napoli Federico II, Napoli, Italy
    J Med Chem 54:3065-75. 2011
    ..Exposure of liver cells to this agent resulted in potent induction of PXR-regulated genes and modulation of FXR-regulated genes, highlighting its pharmacological potential in the treatment of liver disorders...
  40. ncbi A Drosophila model of the Niemann-Pick type C lysosome storage disease: dnpc1a is required for molting and sterol homeostasis
    Xun Huang
    Departments of Developmental Biology, Genetics, and Bioengineering, Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA 94305 5439, USA
    Development 132:5115-24. 2005
    ..We propose that dnpc1a mutants have sterols trapped in aberrant organelles, leading to a shortage of sterol in the endoplasmic reticulum and/or mitochondria ..
  41. ncbi Lipid composition of peroxisomes from the yeast Pichia pastoris grown on different carbon sources
    Tamara Wriessnegger
    Institute of Biochemistry, Graz University of Technology, Petersgasse 12 2, Graz, Austria
    Biochim Biophys Acta 1771:455-61. 2007
    ..In summary, our data provide first insight into biochemical properties of P. pastoris peroxisomal membranes, which may become important for the biotechnological use of this yeast...
  42. pmc Sterols block binding of COPII proteins to SCAP, thereby controlling SCAP sorting in ER
    Peter J Espenshade
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9046, USA
    Proc Natl Acad Sci U S A 99:11694-9. 2002
    b>Sterols inhibit their own synthesis in mammalian cells by blocking the vesicular endoplasmic reticulum-to-Golgi transport of sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP), a sterol-sensing protein ..
  43. ncbi Effect of the structure of natural sterols and sphingolipids on the formation of ordered sphingolipid/sterol domains (rafts). Comparison of cholesterol to plant, fungal, and disease-associated sterols and comparison of sphingomyelin, cerebrosides, and cer
    X Xu
    Department of Biochemistry and Cell Biology, State University of New York at Stony Brook, Stony Brook, New York 11794-5215, USA
    J Biol Chem 276:33540-6. 2001
    ..2000) Biochemistry 39, 844-849). In this study, the domain-promoting activities of various natural sterols were compared with that of cholesterol using both fluorescence quenching and detergent insolubility methods...
  44. ncbi Crucial step in cholesterol homeostasis: sterols promote binding of SCAP to INSIG-1, a membrane protein that facilitates retention of SREBPs in ER
    Tong Yang
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75390, USA
    Cell 110:489-500. 2002
    ..b>Sterols induce binding of SCAP to INSIG-1, as determined by blue native-PAGE, and this is correlated with the inhibition ..
  45. ncbi Sterol esterification in yeast: a two-gene process
    H Yang
    Institute of Human Nutrition, Columbia University College of Physicians and Surgeons, New York, 10032, USA
    Science 272:1353-6. 1996
    ..With the use of a consensus sequence to the yeast and human genes, an additional number of the ACAT gene family was identified in humans...
  46. pmc Osh proteins regulate membrane sterol organization but are not required for sterol movement between the ER and PM
    Alexander G Georgiev
    Department of Biochemistry, Weill Cornell Medical College, New York, NY, USA
    Traffic 12:1341-55. 2011
    ..of radiolabeled ergosterol at the PM was assessed in isolated PM-enriched fractions as well as by extracting sterols from intact cells with methyl-β-cyclodextrin...
  47. ncbi Cholesterol absorption efficiency and sterol metabolism in obesity
    T A Miettinen
    Department of Medicine, Division of Internal Medicine, University of Helsinki, P O Box 340, FIN 00029 HYKS, Helsinki, Finland
    Atherosclerosis 153:241-8. 2000
    ..in healthy lean (BMI <24) and overweight (BMI >31) subjects by measuring serum lipids (including plant sterols, cholestanol and cholesterol precursors), cholesterol absorption % (double-label method), sterol balance and ..
  48. pmc Colimitation of a freshwater herbivore by sterols and polyunsaturated fatty acids
    Dominik Martin-Creuzburg
    Limnological Institute, University of Constance, Mainaustrasse 252, 78464 Konstanz, Germany
    Proc Biol Sci 276:1805-14. 2009
    ..magna on a sterol- and EPA-deficient diet was initially constrained by the absence of sterols. With increased sterol availability, a colimitation by EPA became apparent and when the sterol requirements were ..
  49. pmc Transcriptional regulation of the two sterol esterification genes in the yeast Saccharomyces cerevisiae
    K Jensen-Pergakes
    Department of Biology, Indiana University-Purdue University at Indianapolis, Indianapolis, Indiana 46202, USA
    J Bacteriol 183:4950-7. 2001
    ..cerevisiae transcribes two genes, ARE1 and ARE2, that contribute disproportionately to the esterification of sterols. Are2p is the major enzyme isoform in a wild-type cell growing aerobically...
  50. ncbi A dynamic role for sterols in embryogenesis of Pisum sativum
    Kathrin Schrick
    Keck Graduate Institute of Applied Life Sciences, 535 Watson Drive, Claremont, CA 91711, USA
    Phytochemistry 72:465-75. 2011
    Molecular roles of sterols in plant development remain to be elucidated...
  51. ncbi Antibacterial activity and cytotoxicity analysis of halistanol trisulphate from marine sponge Petromica citrina
    Palloma R Marinho
    Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Av Carlos Chagas Filho, 373, Cidade Universitaria, 21941 590, Rio de Janeiro, Brazil
    J Antimicrob Chemother 67:2396-400. 2012
    ..We performed a chemical and biological characterization of the antibiotic substance from P. citrina and investigated its mode of action on Staphylococcus aureus cells...
  52. pmc A comprehensive method for extraction and quantitative analysis of sterols and secosteroids from human plasma
    Jeffrey G McDonald
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Lipid Res 53:1399-409. 2012
    We describe the development of a method for the extraction and analysis of 62 sterols, oxysterols, and secosteroids from human plasma using a combination of HPLC-MS and GC-MS. Deuterated standards are added to 200 μl of human plasma...
  53. ncbi Sterol synthesis and viability of erg11 (cytochrome P450 lanosterol demethylase) mutations in Saccharomyces cerevisiae and Candida albicans
    M Bard
    Department of Biology, Indiana University Purdue University at Indianapolis 46202 5132
    Lipids 28:963-7. 1993
    ..The erg11 mutants of S. cerevisiae are compared to similar mutants of Candida albicans that are viable in the absence of the erg3 lesion...
  54. ncbi Lipid-induced ER stress: synergistic effects of sterols and saturated fatty acids
    Ludovic Pineau
    Universite de Poitiers, FRE CNRS 3091 Physiologie moléculaire du Transport des Sucres, 86022 Poitiers, France
    Traffic 10:673-90. 2009
    ..The present data are discussed in the context of human diseases that involve lipid deregulation...
  55. ncbi Plant sterols and their derivatives: the current spread of results
    P J Jones
    School of Dietetics and Human Nutrition, McGill University, Quebec, Canada
    Nutr Rev 59:21-4. 2001
    Whereas the cholesterol-lowering action of plant sterols and their derivatives has been well established, more recent results have produced new information concerning the similar effects of various mixtures and dose-response ..
  56. pmc Polyoxygenated sterols from the South China Sea soft coral Sinularia sp
    Rui Li
    Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China
    Mar Drugs 10:1422-32. 2012
    ..collected from the South China Sea led to the isolation of three new polyoxygenated sterols, (3S,23R,24S)-ergost-5-ene-3β,23α,25-triol (1), (24S)-ergostane-6-acetate-3β,5α,6β,25-tetraol (2), (24S)-..
  57. pmc Analysis of bioactive oxysterols in newborn mouse brain by LC/MS
    Anna Meljon
    Institute of Mass Spectrometry, College of Medicine, Swansea, UK
    J Lipid Res 53:2469-83. 2012
    ..The desmosterol metabolites (24Z),26-, (24E),26-, and 7α-hydroxydesmosterol were identified in brain for the first time...
  58. ncbi Lanostane triterpenoids and sterols from Antrodia camphorata
    Hui Chi Huang
    Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, Taiwan
    Phytochemistry 84:177-83. 2012
    ..3,7,11,12,15,23-hexaoxo-5α-lanost-8-en-26-oate, and ethyl 3,7,11,12,15,23-hexaoxo-5α-lanost-8-en-26-oate, two sterols, (14α,22E)-14-hydroxyergosta-7,22-diene-3,6-dione and a steroid named as camphosterol A were isolated from a ..
  59. ncbi Complex formation of amphotericin B in sterol-containing membranes as evidenced by surface plasmon resonance
    Ryota Mouri
    Department of Chemistry, Graduate School of Science, Osaka University, 1 1 Machikaneyama, Toyonaka, Osaka 560 0043, Japan
    Biochemistry 47:7807-15. 2008
    ..These findings support the previous notion that ergosterol stabilizes the membrane-bound assembly of AmB...
  60. pmc Sterols in spermatogenesis and sperm maturation
    Rok Keber
    Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, 1230 Domzale, Slovenia
    J Lipid Res 54:20-33. 2013
    ..However, the exact role of sterols in male reproduction is unknown...
  61. pmc Conservation of the sterol regulatory element-binding protein pathway and its pathobiological importance in Cryptococcus neoformans
    Yun C Chang
    Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Eukaryot Cell 8:1770-9. 2009
    ..These findings suggest that the SREBP pathway is highly conserved in C. neoformans and it serves as an important link between sterol biosynthesis, oxygen sensing, CoCl(2) sensitivity, and virulence in C. neoformans...
  62. ncbi Transcription factors CgUPC2A and CgUPC2B regulate ergosterol biosynthetic genes in Candida glabrata
    Minoru Nagi
    Department of Chemotherapy and Mycoses, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Genes Cells 16:80-9. 2011
    ..These results suggest that in C. glabrata CgUPC2A but not in CgUPC2B is the main transcriptional regulator of the genes responsible for maintaining sterol homeostasis as well as susceptibility to sterol inhibitors...
  63. pmc Structural sterols are involved in both the initiation and tip growth of root hairs in Arabidopsis thaliana
    Miroslav Ovecka
    Core Facility of Cell Imaging and Ultrastructure Research, University of Viena, A 1090 Viena, Austria
    Plant Cell 22:2999-3019. 2010
    Structural sterols are abundant in the plasma membrane of root apex cells in Arabidopsis thaliana...
  64. pmc The sterol-sensing endoplasmic reticulum (ER) membrane protein TRC8 hampers ER to Golgi transport of sterol regulatory element-binding protein-2 (SREBP-2)/SREBP cleavage-activated protein and reduces SREBP-2 cleavage
    Masato Irisawa
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113 8657, Japan
    J Biol Chem 284:28995-9004. 2009
    ....
  65. ncbi Comparison of the interaction of tomatine with mixed monolayers containing phospholipid, egg sphingomyelin, and sterols
    Barry W Walker
    Department of Chemistry and Biochemistry, University of Missouri St Louis, One University Boulevard, Saint Louis, MO 63121, USA
    Biochim Biophys Acta 1778:2244-57. 2008
    ..Brewster angle microscopy (BAM) reveals further evidence for formation of suspected islands of tomatine + cholesterol complexes upon interaction with mixed monolayers of lipid and sterol...
  66. ncbi Synthesis, storage and degradation of neutral lipids in yeast
    Tibor Czabany
    Institute of Biochemistry, Graz University of Technology, Petersgasse 12 2, A 8010 Graz, Austria
    Biochim Biophys Acta 1771:299-309. 2007
    ..The interplay of different subcellular compartments in neutral lipid metabolism, regulatory aspects of this process and cell biological consequences of dysfunctions will be discussed...
  67. ncbi Relative abundance of Delta(5)-sterols in plasma membrane lipids of root-tip cells correlates with aluminum tolerance of rice
    M Shahadat Hossain Khan
    Faculty of Agriculture, Yamagata University, Tsuruoka, Japan
    Physiol Plant 135:73-83. 2009
    ..The tolerant cultivar Rikuu-132 had a lower ratio of phospholipids to Delta(5)-sterols than the sensitive cultivar Rikuu-20, suggesting that the PM of Rikuu-132 is less negatively charged and less ..
  68. pmc Lipid binding requirements for oxysterol-binding protein Kes1 inhibition of autophagy and endosome-trans-Golgi trafficking pathways
    Marissa A LeBlanc
    Department of Pediatrics and Biochemistry and Molecular Biology, Atlantic Research Centre, Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada
    J Biol Chem 285:33875-84. 2010
    ..family of oxysterol-binding proteins found throughout Eukarya united by a β-barrel structure that binds sterols and oxysterols...
  69. pmc Quantifying anomalous intestinal sterol uptake, lymphatic transport, and biliary secretion in Abcg8(-/-) mice
    Helen H Wang
    Department of Medicine, Liver Center and Gastroenterology Division, Beth Israel Deaconess Medical Center, Harvard Medical School, Harvard Digestive Diseases Center, Boston, MA, USA
    Hepatology 45:998-1006. 2007
    ..cholesterol and sitostanol, we performed direct measurements of the absorption and lymphatic transport of these sterols in mice with chronic biliary and lymphatic fistulae, as well as the transport rates of radiolabeled cholesterol ..
  70. ncbi Sterol glycosyltransferases--the enzymes that modify sterols
    Pankaj Chaturvedi
    National Botanical Research Institute Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow, 226001, Uttar Pradesh, India
    Appl Biochem Biotechnol 165:47-68. 2011
    b>Sterols are important components of cell membranes, hormones, signalling molecules and defense-related biotic and abiotic chemicals...
  71. ncbi Marine sponge steroids as nuclear receptor ligands
    Stefano Fiorucci
    Dipartimento di Medicina Clinica e Sperimentale, Nuova Facoltà di Medicina e Chirurgia, 06132 Perugia, Italy
    Trends Pharmacol Sci 33:591-601. 2012
    ..These findings pave the way to the development of novel FXR and PXR agonists and antagonists to target human diseases...
  72. pmc Sterols are mainly in the cytoplasmic leaflet of the plasma membrane and the endocytic recycling compartment in CHO cells
    Mousumi Mondal
    Department of Biochemistry, Weill Cornell Medical College, New York, NY 10065, USA
    Mol Biol Cell 20:581-8. 2009
    ..Using the fluorescent sterols, dehydroergosterol and cholestatrienol, and a variety of fluorescence quenchers, we studied the transbilayer ..
  73. ncbi Requirement of sterols in the life cycle of the nematode Caenorhabditis elegans
    Eugeni V Entchev
    MPI for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Semin Cell Dev Biol 16:175-82. 2005
    ..show that especially two processes in the life cycle of the worm, dauer larva formation and molting, depend on sterols. In both cases, cholesterol or its derivatives seem to act as hormones rather than being structural components of ..
  74. ncbi Effects of sterols on the development and aging of Caenorhabditis elegans
    Eun Young Lee
    Yonsei Proteome Research Center, Biomedical Proteome Research Center, Department of Biochemistry, Yonsei University, Seoul, Korea
    Methods Mol Biol 462:167-79. 2009
    Although Caenorhabditis elegans lacks several components of the de novo sterol biosynthetic pathway, it requires sterols as essential nutrients. Supplemental cholesterol undergoes extensive enzymatic modification in C...
  75. ncbi Identification of the Arabidopsis dry2/sqe1-5 mutant reveals a central role for sterols in drought tolerance and regulation of reactive oxygen species
    David Posé
    Departamento de Biologia Molecular y Bioquimica, Universidad de Malaga, 29071 Malaga, Spain
    Plant J 59:63-76. 2009
    ..As RHD2 NADPH oxidase is de-localized in dry2/sqe1-5 root hairs, we propose that sterols play an essential role in the localization of NADPH oxidases required for regulation of reactive oxygen species, ..
  76. pmc Amphotericin B channels in the bacterial membrane: role of sterol and temperature
    Berenice Venegas
    Centro de Ciencias Fisicas, UNAM, 62251 Cuernavaca, Morelos, Mexico
    Biophys J 85:2323-32. 2003
    ..The change in permeability produced by these channels is greatly improved by sterols; nevertheless, the single channel conductivity remains invariant...
  77. ncbi Towards new ligands of nuclear receptors. Discovery of malaitasterol A, an unique bis-secosterol from marine sponge Theonella swinhoei
    Simona De Marino
    Dipartimento di Chimica delle Sostanze Naturali, Universita di Napoli Federico II, via D Montesano 49, 80131 Napoli, Italy
    Org Biomol Chem 9:4856-62. 2011
    ..The biological characterization of malaitasterol A provided evidence that this compound is a potent agonist of pregnane-X-receptor and its putative binding mode to PXR has been obtained through docking calculations...
  78. ncbi How do sterols determine the antifungal activity of amphotericin B? Free energy of binding between the drug and its membrane targets
    Anna Neumann
    Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdansk University of Technology, Narutowicza St 11 12, 80 233 Gdansk, Poland
    J Am Chem Soc 132:18266-72. 2010
    ..It is commonly accepted that the presence of sterols in the membrane is essential for the AmB biological activity, that is, for the formation of transmembrane ion ..
  79. ncbi A comparison of the LDL-cholesterol lowering efficacy of plant stanols and plant sterols over a continuous dose range: results of a meta-analysis of randomized, placebo-controlled trials
    Kathy Musa-Veloso
    Cantox Health Sciences International, Mississauga, Ontario, Canada
    Prostaglandins Leukot Essent Fatty Acids 85:9-28. 2011
    To determine if plant stanols and plant sterols differ with respect to their low-density lipoprotein cholesterol (LDL-CH) lowering efficacies across a continuous dose range.
  80. pmc Overlapping functions of the yeast oxysterol-binding protein homologues
    C T Beh
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3202, USA
    Genetics 157:1117-40. 2001
    ..Our findings indicated that OSH genes performed a multitude of nonessential roles defined by specific subsets of the genes and that most shared at least one essential role potentially linked to changes in sterol lipid levels...
  81. pmc Discovery that theonellasterol a marine sponge sterol is a highly selective FXR antagonist that protects against liver injury in cholestasis
    Barbara Renga
    Dipartimento di Medicina Clinica e Sperimentale, Nuova Facoltà di Medicina e Chirurgia, Universita di Perugia, S Andrea delle Fratte, Perugia, Italy
    PLoS ONE 7:e30443. 2012
    ..Theonellasterol is a 4-methylene-24-ethylsteroid isolated from the marine sponge Theonella swinhoei. Here, we have characterized the activity of this theonellasterol on FXR-regulated genes and biological functions...
  82. ncbi Potential beneficial effects of marine algal sterols on human health
    Se Kwon Kim
    Marine Bioprocess Research Center, Pukyong National University, Busan, Republic of Korea
    Adv Food Nutr Res 64:191-8. 2011
    ..b>Sterols are important structural component of cell membranes...
  83. pmc Chemical combinations elucidate pathway interactions and regulation relevant to Hepatitis C replication
    Christopher M Owens
    CombinatoRx, Incorporated, Cambridge, MA 02142, USA
    Mol Syst Biol 6:375. 2010
    ..Our findings show how combination chemical genetics can reveal critical pathway connections relevant to viral replication, and can identify potential treatments with an increased therapeutic window...
  84. pmc The use of the Dhcr7 knockout mouse to accurately determine the origin of fetal sterols
    G S Tint
    Research Service, Department of Veterans Affairs Medical Center, East Orange, NJ 07018, USA
    J Lipid Res 47:1535-41. 2006
    ..Dhcr7) that cannot convert 7-dehydrocholesterol to cholesterol were used to identify the origin of fetal sterols. Because their heterozygous mothers synthesize cholesterol normally, virtually all sterols found in a Dhcr7 ..
  85. pmc Sterol-induced dislocation of 3-hydroxy-3-methylglutaryl coenzyme A reductase from endoplasmic reticulum membranes into the cytosol through a subcellular compartment resembling lipid droplets
    Isamu Z Hartman
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 74390 9046, USA
    J Biol Chem 285:19288-98. 2010
    ..Genetic, biochemical, and localization studies suggest a model in which reductase is dislodged into the cytosol from an ER subdomain closely associated with lipid droplets...
  86. ncbi Cholesterol and the interaction of proteins with membrane domains
    Richard M Epand
    Department of Biochemistry and Biomedical Sciences, Health Sciences Centre, McMaster University, 1200 Main Street, Hamilton, Ont, Canada L8N 3Z5
    Prog Lipid Res 45:279-94. 2006
    ..One of these proteins is StAR-D1, that also has a mitochondrial targeting sequence and plays an important role in delivering cholesterol to the mitochondria of steroidogenic cells...
  87. pmc Specific sterols required for the internalization step of endocytosis in yeast
    A L Munn
    Biozentrum of the University of Basel, CH 4056 Basel, Switzerland
    Mol Biol Cell 10:3943-57. 1999
    b>Sterols are major components of the plasma membrane, but their functions in this membrane are not well understood...
  88. ncbi Why does Kluyveromyces lactis not grow under anaerobic conditions? Comparison of essential anaerobic genes of Saccharomyces cerevisiae with the Kluyveromyces lactis genome
    I S Ishtar Snoek
    Institute of Biology Leiden, Leiden University, Leiden, The Netherlands
    FEMS Yeast Res 6:393-403. 2006
    ..cerevisiae, which yielded 20 genes that are missing in K. lactis, we hypothesize that lack of import of sterols might be one of the more important reasons that K. lactis cannot grow in the absence of oxygen.
  89. ncbi Genetic variation in ABC G5/G8 and NPC1L1 impact cholesterol response to plant sterols in hypercholesterolemic men
    Hai L Zhao
    Richardson Centre for Functional Foods and Nutraceuticals, University of Manitoba, 196 Innovation Drive, Winnipeg, MB, R3T 6C5, Canada
    Lipids 43:1155-64. 2008
    ..Results suggest that genetic and metabolic biomarkers together may predict inter-individual lipid level responsiveness to PS-intervention, and thus could be useful in devising individualized cholesterol lowering strategies...
  90. ncbi Transport-dependent proteolysis of SREBP: relocation of site-1 protease from Golgi to ER obviates the need for SREBP transport to Golgi
    R A DeBose-Boyd
    Department of Molecular Genetics, University of Texas, Southwestern Medical Center, Dallas 75390 9046, USA
    Cell 99:703-12. 1999
    ..b>Sterols block this transport and abolish cleavage...
  91. ncbi Vesicle fluctuation analysis of the effects of sterols on membrane bending rigidity
    Jonas Henriksen
    MEMPHYS Centre for Biomembrane Physics, Department of Chemistry, Technical University of Denmark, 2800, Lyngby, Denmark
    Eur Biophys J 33:732-41. 2004
    b>Sterols are regulators of both biological function and structure. The role of cholesterol in promoting the structural and mechanical stability of membranes is widely recognized...
  92. ncbi Antifungal agents: mode of action in yeast cells
    A J Carrillo-Munoz
    Department of Microbiology, ACIA, Barcelona, Spain
    Rev Esp Quimioter 19:130-9. 2006
    ..The interaction of amphotericin B with ergosterol and other membrane sterols results in the production of aqueous pores of drug and the ergosterol biosynthetic pathway is the target of the ..
  93. pmc Sterols regulate cycling of SREBP cleavage-activating protein (SCAP) between endoplasmic reticulum and Golgi
    A Nohturfft
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75235, USA
    Proc Natl Acad Sci U S A 96:11235-40. 1999
    ..Here, we provide evidence that sterols act by inhibiting the cycling of SCAP between the ER and Golgi...
  94. pmc Nuclear envelope remnants: fluid membranes enriched in sterols and polyphosphoinositides
    Marie Garnier-Lhomme
    Cell Biophysics Laboratory, Lincoln s Inn Fields Laboratories, Cancer Research UK, London, United Kingdom
    PLoS ONE 4:e4255. 2009
    ..Remnants are conserved from annelid to mammalian sperm...
  95. ncbi Nature of sterols affects plasma membrane behavior and yeast survival during dehydration
    Sebastien Dupont
    Laboratoire de Génie des Procédés Microbiologiques et Alimentaires, Université de Bourgogne AgroSup Dijon, 1, Esplanade Erasme, 21000 Dijon, France
    Biochim Biophys Acta 1808:1520-8. 2011
    The plasma membrane (PM) is a main site of injury during osmotic perturbation. Sterols, major lipids of the PM structure in eukaryotes, are thought to play a role in ensuring the stability of the lipid bilayer during physicochemical ..
  96. pmc Dislocation of HMG-CoA reductase and Insig-1, two polytopic endoplasmic reticulum proteins, en route to proteasomal degradation
    Gil S Leichner
    Department of Human Genetics and Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    Mol Biol Cell 20:3330-41. 2009
    The endoplasmic reticulum (ER) glycoprotein HMG-CoA reductase (HMGR) catalyzes the rate-limiting step in sterols biosynthesis...
  97. ncbi Oxysterol binding proteins: in more than one place at one time?
    Vesa M Olkkonen
    Department of Molecular Medicine, National Public Health Institute, Helsinki, Finland
    Biochem Cell Biol 82:87-98. 2004
    ..Specifically, we suggest a model in which a general property of ORPs is to function at membrane contact sites, specialized zones of communication between two different organelles...
  98. ncbi Relationship between cholesterol synthesis and intestinal absorption is associated with cardiovascular risk
    Oliver Weingärtner
    Klinik fur Innere Medizin III, Kardiologie, Angiologie und Internistische Intensivmedizin, Universitatsklinikum des Saarlandes, Kirrberger Strasse, Gebäude 40, D 66421 Homburg Saar, Germany
    Atherosclerosis 210:362-5. 2010
    ..Therefore, prospective clinical trials are needed to evaluate whether subjects with altered cholesterol homeostasis may benefit from treatment strategies that reduce cholesterol absorption in addition to statin treatment...
  99. pmc 7-Dehydrocholesterol-dependent proteolysis of HMG-CoA reductase suppresses sterol biosynthesis in a mouse model of Smith-Lemli-Opitz/RSH syndrome
    B U Fitzky
    Institute of Biochemical Pharmacology, Innsbruck, Austria
    J Clin Invest 108:905-15. 2001
    ..This effect might exacerbate abnormal development in SLOS by increasing the fetal cholesterol deficiency...
  100. pmc Amiodarone destabilizes intracellular Ca2+ homeostasis and biosynthesis of sterols in Leishmania mexicana
    Xenón Serrano-Martín
    Centro de Biociencias y Medicina Molecular, Instituto de Estudios Avanzados, Carretera Nacional Hoyo de la Puerta, Baruta, Caracas, Venezuela
    Antimicrob Agents Chemother 53:1403-10. 2009
    ..On the other hand, analysis of the free sterols in promastigotes incubated with amiodarone showed that this drug also affected the biosynthesis of 5-..
  101. ncbi Polyoxygenated sterols from the Formosan soft coral Sinularia gibberosa
    Atallah F Ahmed
    Department of Marine Biotechnology and Resources, National Sun Yat Sen University, Kaohsiung 804, Taiwan, Republic of China
    J Nat Prod 69:1275-9. 2006
    ..the EtOH extract of Sinularia gibberosa Tixier-Durivault has led to the isolation of three new polyoxygenated sterols, gibberoketosterols B (1) and C (2) and gibberoepoxysterol (3), along with two known steroids, gibberoketosterol (..

Research Grants67

  1. Dietary Cholesterol and Defects in Cholesterol Synthesis
    ROBERT DAVID STEINER; Fiscal Year: 2013
    ..e. sterols and oxysterols, ERG, and brain MRI), in comparison with patients receiving only cholesterol supplementation...
  2. Regulation of Metabolism by Dietary Sterols
    SHAILENDRA BHANUBHAI PATEL; Fiscal Year: 2013
    ..This pathway has shed light onto how dietary sterols (cholesterol and plant sterols) traffic at the intestinal and hepatocyte level regulated by the proteins ABCG5/..
  3. Pathogenesis/Treatment-Inherited Cholesterol Deficiency
    Gordon L Watson; Fiscal Year: 2013
    ..At various times brains will be analyzed for sterols, DHCR7 DNA and mRNA, and the distribution of cells expressing FLAG-DHCR7...
  4. Human Stem Cell Model of Niemann Pick Type C
    Lawrence S Goldstein; Fiscal Year: 2010
    ..The result is a severe lipidosis characterized by massive accumulation of lysosomal sterols and other lipids that ultimately cause cell death...
  5. Investigating the Mechanisms Regulating RORgamma Activity
    LAURA ANN SOLT; Fiscal Year: 2013
    ..We have identified endogenous, functional ligands (7- oxygenated sterols) for ROR3...
  6. Receptor-mediated Endocytosis-Mechanism and Function
    Frederick R Maxfield; Fiscal Year: 2013
    ..In Aim IV intracellular sterol transport and distribution will be characterized using fluorescent sterols and biochemical analyses...
  7. Targeting PCSK9 Transcription to Combat Hypercholesterolemia by Berberine
    Jingwen Liu; Fiscal Year: 2013
    ..of the PCSK9-LDLR pathway in mediating LDL-C clearance is that their transcription is coordinately regulated by sterols through a common sterol regulatory element (SRE) motif embedded in their gene promoters and is co-induced by ..
  8. Alternative Splicing in Regulation of Cholesterol Synthesis and Uptake
    Marisa Wong Medina; Fiscal Year: 2013
    ..in cholesterol metabolism (HMGCR, LDLR, HMG-CoA synthase and mevalonate kinase) is coordinately regulated by sterols such that sterol loading increases alternative splicing while sterol depletion suppresses alternative splicing...
  9. Mechanistic Studies of the Function of Desmosterol Homeostasis in HCV Replication
    VALERIE ANNE AGUSTINO VILLAREAL; Fiscal Year: 2013
    ..Bloch branch of cholesterol biosynthesis, without perturbing the steady-state abundance of cholesterol or other sterols in the pathway...
  10. Development of novel therapeutics for a neglected tropical disease leishmaniasis
    ABHAY R contact SATOSKAR; Fiscal Year: 2010
    ..already led to the identification of several compounds with leishmanicidal activity in PARE, including two novel sterols. More bioactive molecules are expected to be isolated from the plant before the completion of this R21 project in ..
  11. Role of Sterols and Insulin in Cardiac Autonomic Response
    JONAS BERNARD GALPER; Fiscal Year: 2013
    ....
  12. Cholesterol Uptake by Cryptosporidium
    Isabelle Coppens; Fiscal Year: 2010
    ..We documented the presence of sterols on the parasite's plasma membrane and organelles indicating that Cryptosporidium is able to internalize host ..
  13. CHARACTERIZATION OF A NEW FAMILY OF PROTEIN KINASES
    Kirill M Popov; Fiscal Year: 2012
    ..goes beyond its role in energy production because it also serves as a source of carbon for biosynthesis of sterols and fatty acid and provides the general means to control the tissue levels of pyruvate...
  14. Nutritional Supplement to Reduce Triglycerides and Cholesterol
    Sharon L Miller; Fiscal Year: 2010
    ..acids (EAAs) and the amino acid arginine that have been shown to lower circulating TGs and liver fat, and plant sterols that have been shown to lower total and LDL cholesterol...
  15. Mass Spectrometry Strategies to Identify Lipid Biomarkers of Disease
    GAVIN EDMUND REID; Fiscal Year: 2012
    ..Lipids are a diverse group of compounds, including fatty acyls, sterols, glycerolipids, glycerophospholipids and sphingolipids that play key biological roles as the main structural ..
  16. Mass Spectrometry Strategies to Identify Lipid Biomarkers of Disease
    GAVIN EDMUND REID; Fiscal Year: 2011
    ..Lipids are a diverse group of compounds, including fatty acyls, sterols, glycerolipids, glycerophospholipids and sphingolipids that play key biological roles as the main structural ..
  17. Intracellular transfer of cholesterol in mosquitoes
    Que Lan; Fiscal Year: 2010
    ..Specific aim 2: Examine the roles of AeSCP-2 in coordinating the absorption of sterols and cholesterol using newly discovered inhibitors and RNA interference (RNAi) in a mosquito organism model system...
  18. Role of Syntaxin 6 Regulated Post-Golgi Trafficking in Angiogenesis
    Amit Choudhury; Fiscal Year: 2012
    ..Membrane rafts" are regions of the plasma membrane that are enriched in sphingolipids and sterols. These domains are also enriched in signaling proteins including certain kinases, integrins and vascular ..
  19. Molecular Mechanism of Allosteric Modulation of the Oxytocin Receptor by Sterols
    VADIM GENNADYEVICH CHEREZOV; Fiscal Year: 2013
    ..High resolution crystal structures give insights into mechanism of signal transduction and provide necessary templates for the rational design of a new generation of more efficient and safe drugs. ..
  20. Ordered Membrane Domain Formation and Function in Pathogenic Bacteria
    Erwin London; Fiscal Year: 2013
    ..burgdorferi membranes in order to determine whether the ability of sterols and other lipids to form ordered domains in vitro is necessary and sufficient for them to form membrane domains ..
  21. Dissecting ER-Associated Degradation of a Membrane Protein in Drosophila S2 Cells
    RUSSELL ALFRED DEBOSE-BOYD; Fiscal Year: 2013
    ..Excess sterols cause binding of the membrane domain of reductase to ER membrane proteins called Insig-1 and Insig-2, resulting ..
  22. HUMAN CYTOSOLIC SULFOTRANSFERASES
    Charles N Falany; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Sulfation is a major conjugation reaction for many endogenous sterols, catecholamines, bile acids and thyroid hormones as well as xenobiotics and therapeutic drugs...
  23. The Role of Statins in Cutaneous Wound Healing
    Marjana Tomic-Canic; Fiscal Year: 2012
    ..pyrophosphate (FPP), an important branch-point intermediate in the mevalonate pathway, essential for synthesis of sterols and isoprenylated cellular metabolites leading to cholesterol synthesis...
  24. Role of Star Proteins in Bile Acid and Cholesterol Metabolism
    William M Pandak; Fiscal Year: 2012
    ..a family of proteins containing a StarD1 homologue domain that potentially is capable of binding and transporting sterols within cells...
  25. Brain Lipid Rafts In Cholesterol Biosynthesis Disorders
    Hongwei Yu; Fiscal Year: 2011
    ..sterol-enriched raft domains in the former and sterol-depleted raft domains in the latter;2) the partitioning of sterols and raft-associated proteins into raft membranes were associated with the expression of Dhcr24, suggesting that ..
  26. Targeting Sterol Gene SC4MOL and EGFR as Synergistic Anti-Cancer Strategy
    Igor Astsaturov; Fiscal Year: 2013
    ..This proposal is significant because it will provide fundamentally new knowledge on how the metabolism of sterols regulates signaling activity of essential cancer receptors such as EGFR...
  27. Structure and Function of the Trypanosome Flagellar Membrane
    David M Engman; Fiscal Year: 2013
    ..in conformation, (ii) their localization to the flagellar membrane is dependent on the concentration of membrane sterols, (iii) they appear to function through the calcium-dependent binding and presumed regulation of partner proteins, ..
  28. Thermo TSQ Quantum Access Mass Spectrometer
    Yusuf Awni Hannun; Fiscal Year: 2010
    ..Major lipid classes include triacylglycerols, phospholipids, glycolipids, sphingolipids, sterols, waxes and fatty acids...
  29. Sterol and Coenzyme Q metabolism in C. elegans
    Fernando Gomez; Fiscal Year: 2010
    ..However, supplementing worm media with 4-methyl sterols such as lophenol may be able to rescue these coq-3 deficient worms...
  30. Sterol Biosynthesis in Trypanosomatid Parasites
    Frederick Buckner; Fiscal Year: 2004
    ..Leishmania species and Trypanosoma cruzi synthesize membrane sterols similar to those of fungi...
  31. Variations in lipid homeostasis and signaling affect alpha-synuclein pathobiology
    Daniel J Termine; Fiscal Year: 2012
    ..The amount of sphingolipids and sterols in alpha-syn expressing DA neurons will be quantified as well.
  32. PROTEIN DEGRADATION AND CHOLESTEROL REGULATION
    Randolph Y Hampton; Fiscal Year: 2013
    ..PUBLIC HEALTH RELEVANCE: HMG-CoA reductase (HMGR) is a key enzyme in the cholesterol pathway, from which sterols and a wide variety of other essential molecules are produced...
  33. DIETARY LIPIDS--EFFECTS ON LIPID-LIPOPROTEIN METABOLISM
    D Illingworth; Fiscal Year: 1991
    ..The influence of sterols on plasma lipid and lipoprotein concentrations will be examined in normal and hypercholesterolemic subjects fed ..
  34. Sulfotransferase Expression: Implications for Toxicity
    MELISSA A RUNGE-MORRIS; Fiscal Year: 2012
    ..SULT2A1 catalyzes the sulfonation of endogenous sterols, thereby modulating the expression of LXR target genes, including SULT2A1 itself...
  35. Smith-Lemli-Opitz syndrome and Inborn Errors of Cholesterol Synthesis
    ROBERT DAVID STEINER; Fiscal Year: 2013
    ..Since then, much progress has been made in the field of sterols and rare diseases so as to warrant another conference that will bring together scientists, clinical investigators,..
  36. THE YEAST ERGOSTEROL PATHWAY
    Martin Bard; Fiscal Year: 2007
    ..Two of the most widely used antifungal classes, the polyenes (amphotericin B) target plasma membrane-bound sterols and the azoles (fluconazole, and itraconazole) target the cytochrome P450-mediated step, lanosterol C-14 ..
  37. UV, CD, and NMR studies of Amphotericin B in nanoscale discoidal lipid bilayers
    THOMAS MICHAEL ANDERSON; Fiscal Year: 2013
    ..Experiments will probe the interaction of amphotericin B with cell membranes and sterols present therein to advance understanding of the fundamental mechanism of AmB effectiveness and toxicity...
  38. Regulation of Cellular Cholesterol Homeostasis
    PETER JOHN ESPENSHADE; Fiscal Year: 2013
    ..Interestingly, sterols regulate Sre1 activity through a novel mechanism in fission yeast, and evidence also indicates that Sre1 cleavage ..
  39. YEAST ERGOSTEROL PATHWAY--NEW ANTIFUNGAL TARGET SITES
    Martin Bard; Fiscal Year: 1999
    ..Two of the most widely used antifungal classes, the polyenes (amphotericin B) target plasma membrane-bound sterols and the new classes of azoles (ketoconazole, fluconazole, and itraconazole) target the cytochrome P450-mediated ..
  40. SEEKING THE YEAST SPARKING STEROL
    Martin Bard; Fiscal Year: 1991
    ..Two of the most commonly used antifungals are the polyenes (which interfere with membrane sterols) and azoles (which affect sterol biosynthesis)...
  41. MRI-BASED VIRTUAL BONE BIOPSY IN RENAL OSTEODYSTROPHY
    Felix Wehrli; Fiscal Year: 2001
    ..may be due to therapies aimed at preventing OF, such as calcium-containing phosphate binders and active vitamin D sterols. Current studies are focusing on the development of new strategies to prevent OF without suppressing normal bone ..
  42. Older Persons & Drugs: race, gender, and age effects
    Janice B Schwartz; Fiscal Year: 2010
    ..D3 on complete lipid profiles (directly measured cholesterol subfractions, lipoproteins and non-cholesterol sterols (HDL, LDL, VLDL, IDL, and Lp(a), HDL2, HDL3, and LDL1-LDL4, LDL subclass pattern (A, A/B or B) and remnant ..
  43. THE METABOLISM OF STEROLS FROM SHELLFISH AND ALGAE
    WILLIAM CONNOR; Fiscal Year: 1980
    ..is usually focused upon cholesterol as the chief dietary sterol of human beings, one half or more of the total sterols consumed by man constitute many sterols other than cholesterol. These include sterols from plants and seafoods...
  44. MEMBRANE DYNAMICS AND STEROL BIOSYNTHESIS IN YEAST
    Robin Wright; Fiscal Year: 1999
    ..cellular membranes is required for at least two processes: regulation of the protein's half-life in response to sterols and induction of specialized membrane synthesis in response to HMG-CoA reductase levels...
  45. SPHINGOLIPIDS AND STEROLS--METABOLISM AND INTERACTIONS
    Seiichi Matsuda; Fiscal Year: 2000
    This research program concerns multiple ongoing projects dealing with the biochemistry, chemistry, and actions of sterols, sphingolipid bases (and their derivatives), and isoprenoid alcohols and acids as well as potential ..
  46. BREAST CANCER RISK--VEGETABLES AND SERUM PHYTOSTEROLS
    Paola Muti; Fiscal Year: 2000
    DESCRIPTION: (Applicant's Description) Phytosterols are plant sterols which are structurally similar to cholesterol. In experimental studies, phytosterols and in particular b-sitosterol showed anti-carcinogenic properties...
  47. Sterol Metabolism in Pneumocystis
    EDNA KANESHIRO; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Several drugs that interact with membrane sterols or inhibit their syntheses are effective in clearing fungal infections but Pneumocystis is not cleared by many of these therapies. P...
  48. Role of ABCG5 and ABCG8 in Sterol Metabolism
    HELEN HASKELL HOBBS; Fiscal Year: 2013
    ..mechanism by which the ATP-binding cassette halftransporters, ABCG5 and ABCG8 (G5G8), mediate the transport of sterols across membranes...
  49. METABOLISM OF NEUTRAL STEROLS
    William Elliott; Fiscal Year: 1990
    ..Quantitatively, the major metabolites and excretory products of sterols in mammals are the bile acids, but the details of biosynthesis and its regulation of these acids are not ..
  50. Formulated Phytosterols - A New Treatment For Benign Prostatic Hyperplasia
    CURTIS SPILBURG; Fiscal Year: 2006
    ..Unfortunately, the sterols contained in many of these products are of questionable efficacy, most likely because they are insoluble in water ..
  51. Sitosterolemia
    Gerald Salen; Fiscal Year: 2003
    ..Chemically, plant sterols (sitosterol, stigmasterol, campesterol, and avenosterol) and their respective 5alpha-dihydro derivatives (..
  52. DIETARY FAT, PLASMA LIPIDS AND OTHER CHD RISK FACTORS.
    Alice Lichtenstein; Fiscal Year: 2003
    ..and B; Lp[a]), immune function, and prostaglandin levels, and 2) to address unresolved issues related to plant sterols, including the relative efficacy of currently available preparations, impact of the fat and cholesterol content ..
  53. LIPID AND LIPOPROTEIN METABOLISM IN INSECTS
    Michael Wells; Fiscal Year: 2005
    ..Dietary cholesterol, or sterols that can be converted to cholesterol, are critical for insects because they cannot make sterols de riovo...
  54. NUTRITIONAL INFLUENCES ON COLON CANCER IN THE TAMARIN
    Lynne Ausman; Fiscal Year: 1991
    ..North Americans consuming high fat diets have increased levels of fecal bile acids and neutral sterols which have been implicated as promoting factors in human colon cancer...
  55. VITAMIN D BINDING PROTEIN
    Nancy Cooke; Fiscal Year: 2003
    ..Multiple functions have been attributed to DBP. It binds vitamin D sterols facilitating their serum transport; it sequesters G-actin released to the serum after cellular damage and along ..
  56. ORAL MUCOSAL CELL RESPONSES TO DENTAL RESIN COMPONENTS
    George Schuster; Fiscal Year: 1999
    ..Changes in sterols and levels of methylated phospholipid alter a variety of membrane-associated functions...
  57. CELLULAR METABOLISM IN RESPONSE TO HYPERCHOLESTEROLEMIA
    Judah Weinberger; Fiscal Year: 1993
    ..will be performed in nuclear and whole cell extracts from sterol-starved cells and from cells grown in abundant sterols. Site directed mutagenesis of the sterol response element(s) will be performed to correlate the binding activity ..
  58. Agilent 6890 GC/5973 mass detector for Profiling
    Roger Davis; Fiscal Year: 2005
    ..is to seek funds to procure a GC/mass spectrometer allowing us to analyze cells, tissues and isolates for lipids, sterols and metabolites...
  59. NOVEL INHIBITORS OF CHOLESTEROL BIOSYNTHESIS
    Thomas Spencer; Fiscal Year: 1990
    ..be continued to gain further understanding of the biochemistry of 24(S),25-epoxycholesterol and other oxygenated sterols. Recent evidence has been obtained that this epoxy sterol occurs naturally in human liver tissue and in cultured ..
  60. Role of ABCG5 and ABCG8 in Sterol Metabolism
    Helen Hobbs; Fiscal Year: 2007
    ..The basic pathways of sterol transport in the body have been well defined: sterols enter the body via endogenous synthesis and intestinal absorption and are excreted via the liver into the bile, ..
  61. MEVALONATED METABOLIZING ENZYMES & THEIR INBORN DEFECTS
    Jung Ja Kim; Fiscal Year: 2003
    DESCRIPTION: Mevalonic acid is a key intermediate in the biosynthesis of cellular isoprenoids and sterols. Three enzymes, mevalonate kinase, phosphomevalonate kinase, and mevalonate 5-pyrophosphate decarboxylase, are required to ..
  62. Cytokine-Mediated Regulation of Cholesterogenesis
    Ishaiahu Shechter; Fiscal Year: 2005
    ..metabolic pathway committed to cholesterol biosynthesis and its activity regulates the flux of intermediates to sterols. SQS is regulated by sterols, lipopolysaccharide (LPS), and the pro-inflammatory cytokines tumor necrosis factor-..
  63. APPLICATION OF CHEMICAL METHODS TO STEROID METABOLISM
    Seymour Lieberman; Fiscal Year: 1991
    ..Of specific concern are those reactions by which C/27-sterols are converted into C/21-steroids (e.g...
  64. The isoprenoid pathway from Toxoplasma gondii
    Silvia Moreno; Fiscal Year: 2007
    ..enzyme farnesyl pyrophosphate synthase (FPPS) plays a central role by producing FPP, an important precursor of sterols, dolichols, ubiquinones, and prenylated proteins...
  65. HEME OXYGENASE 1 GENE REGULATION IN OXIDANT RENAL INJURY
    Anupam Agarwal; Fiscal Year: 2000
    ..consisting of several chemically distinct components including fatty acid hydroperoxides, modified apoB, oxidized sterols and phospholipids...
  66. CRYSTALLOGRAPHIC STRUCTURE OF MAMMALIAN MEMBRANE P450
    C Stout; Fiscal Year: 2002
    ..These reactions include pathways for the purging of environmental toxins and carcinogens, the synthesis of sterols, steroids and prostanoids and, in some cases, the metabolic activation of chemical carcinogens...
  67. STEROL DEPENDENT REGULATION OF MEVALONATE SYNTHESIS
    John Watson; Fiscal Year: 1991
    ..We will use a mutant CHO cell line, unable to demethylate 4 alpha-methyl sterols, as our experimental model...