membrane lipids


Summary: Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.

Top Publications

  1. ncbi Membrane curvature and mechanisms of dynamic cell membrane remodelling
    Harvey T McMahon
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nature 438:590-6. 2005
  2. ncbi Direct observation of the nanoscale dynamics of membrane lipids in a living cell
    Christian Eggeling
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nature 457:1159-62. 2009
  3. pmc Emerging roles for lipids in shaping membrane-protein function
    Rob Phillips
    Department of Applied Physics, California Institute of Technology, Pasadena, California 91125, USA
    Nature 459:379-85. 2009
  4. ncbi Atomic structure of a voltage-dependent K+ channel in a lipid membrane-like environment
    Stephen B Long
    Howard Hughes Medical Institute, Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, 1230 York Avenue, New York, New York 10065, USA
    Nature 450:376-82. 2007
  5. ncbi Profiling membrane lipids in plant stress responses. Role of phospholipase D alpha in freezing-induced lipid changes in Arabidopsis
    Ruth Welti
    Division of Biology, Ackert Hall, Kansas State University, Manhattan, Kansas 66506, USA
    J Biol Chem 277:31994-2002. 2002
  6. ncbi Alum interaction with dendritic cell membrane lipids is essential for its adjuvanticity
    Tracy L Flach
    Immunology Research Group, Department of Microbiology and Infectious Diseases, and Snyder Institute, University of Calgary, Calgary, Alberta, Canada
    Nat Med 17:479-87. 2011
  7. ncbi Curvature, lipid packing, and electrostatics of membrane organelles: defining cellular territories in determining specificity
    Joëlle Bigay
    Institut de Pharmacologie Moleculaire et Cellulaire, Université de Nice Sophia Antipolis et CNRS, 06560 Valbonne, France
    Dev Cell 23:886-95. 2012
  8. ncbi Membranes are more mosaic than fluid
    Donald M Engelman
    Department of Molecular Biophysics and Biochemistry, Yale University, Box 208114, New Haven, Connecticut 06520 8114, USA
    Nature 438:578-80. 2005
  9. pmc Sensing voltage across lipid membranes
    Kenton J Swartz
    Porter Neuroscience Research Center, Molecular Physiology and Biophysics Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 456:891-7. 2008
  10. pmc Membrane cholesterol, lateral mobility, and the phosphatidylinositol 4,5-bisphosphate-dependent organization of cell actin
    Jeanne Kwik
    Biology Department, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 100:13964-9. 2003

Detail Information

Publications307 found, 100 shown here

  1. ncbi Membrane curvature and mechanisms of dynamic cell membrane remodelling
    Harvey T McMahon
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nature 438:590-6. 2005
    ..This is seen during vesicle budding and in the formation of microenvironments. On a larger scale, membrane curvature is a prime player in growth, division and movement...
  2. ncbi Direct observation of the nanoscale dynamics of membrane lipids in a living cell
    Christian Eggeling
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nature 457:1159-62. 2009
    ..The non-invasive optical recording of molecular time traces and fluctuation data in tunable nanoscale domains is a powerful new approach to study the dynamics of biomolecules in living cells...
  3. pmc Emerging roles for lipids in shaping membrane-protein function
    Rob Phillips
    Department of Applied Physics, California Institute of Technology, Pasadena, California 91125, USA
    Nature 459:379-85. 2009
    ..The free-energy cost of such perturbations can be estimated quantitatively, and measurements of channel gating in model systems of membrane proteins with their lipid partners are now confirming predictions of simple models...
  4. ncbi Atomic structure of a voltage-dependent K+ channel in a lipid membrane-like environment
    Stephen B Long
    Howard Hughes Medical Institute, Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, 1230 York Avenue, New York, New York 10065, USA
    Nature 450:376-82. 2007
    ..The detailed structure, which can be compared directly to a large body of functional data, explains charge stabilization within the membrane and suggests a mechanism for voltage-sensor movements and pore gating...
  5. ncbi Profiling membrane lipids in plant stress responses. Role of phospholipase D alpha in freezing-induced lipid changes in Arabidopsis
    Ruth Welti
    Division of Biology, Ackert Hall, Kansas State University, Manhattan, Kansas 66506, USA
    J Biol Chem 277:31994-2002. 2002
  6. ncbi Alum interaction with dendritic cell membrane lipids is essential for its adjuvanticity
    Tracy L Flach
    Immunology Research Group, Department of Microbiology and Infectious Diseases, and Snyder Institute, University of Calgary, Calgary, Alberta, Canada
    Nat Med 17:479-87. 2011
    ..Here we report that, independent of inflammasome and membrane proteins, alum binds dendritic cell (DC) plasma membrane lipids with substantial force...
  7. ncbi Curvature, lipid packing, and electrostatics of membrane organelles: defining cellular territories in determining specificity
    Joëlle Bigay
    Institut de Pharmacologie Moleculaire et Cellulaire, Université de Nice Sophia Antipolis et CNRS, 06560 Valbonne, France
    Dev Cell 23:886-95. 2012
    ..The contrasting features of these territories provide specificity for reactions occurring along the secretory pathway, on the plasma membrane, and also on lipid droplets and autophagosomes...
  8. ncbi Membranes are more mosaic than fluid
    Donald M Engelman
    Department of Molecular Biophysics and Biochemistry, Yale University, Box 208114, New Haven, Connecticut 06520 8114, USA
    Nature 438:578-80. 2005
  9. pmc Sensing voltage across lipid membranes
    Kenton J Swartz
    Porter Neuroscience Research Center, Molecular Physiology and Biophysics Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 456:891-7. 2008
    ..Although further structures are needed to understand the conformational changes that occur during voltage sensing, the available data help to frame several key concepts that are fundamental to the mechanism of voltage sensing...
  10. pmc Membrane cholesterol, lateral mobility, and the phosphatidylinositol 4,5-bisphosphate-dependent organization of cell actin
    Jeanne Kwik
    Biology Department, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 100:13964-9. 2003
    ..Consistent with this observation, agents that sequester plasma membrane PI(4,5)P2 mimic the effects of cholesterol depletion on actin organization and on lateral mobility...
  11. ncbi Docosahexaenoic acid: membrane properties of a unique fatty acid
    William Stillwell
    Department of Biology, Indiana University Purdue University Indianapolis, 723 W Michigan Street, Indianapolis, IN 46202 5132, USA
    Chem Phys Lipids 126:1-27. 2003
    ..It is concluded that DHA's interaction with other membrane lipids, particularly cholesterol, may play a prominent role in modulating the local structure and function of cell ..
  12. pmc Interplay of proteins and lipids in generating membrane curvature
    Todd R Graham
    Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235, USA
    Curr Opin Cell Biol 22:430-6. 2010
    ..A model of clathrin-mediated vesicle budding from the trans-Golgi network is described to illustrate the interplay and mutual reinforcement of different mechanisms for generating membrane curvature...
  13. pmc ArfGAP1 responds to membrane curvature through the folding of a lipid packing sensor motif
    Joëlle Bigay
    CNRS, Institut de Pharmacologie Moleculaire et Cellulaire, Sophia Antipolis, France
    EMBO J 24:2244-53. 2005
    ..This helix differs from classical amphipathic helices by the abundance of serine and threonine residues on its polar face...
  14. pmc Mycobacteria manipulate macrophage recruitment through coordinated use of membrane lipids
    C J Cambier
    Department of Immunology, University of Washington, Seattle, Washington 98195, USA
    Nature 505:218-22. 2014
  15. pmc Effects of membrane lipids on the activity and processivity of purified γ-secretase
    Oliver Holmes
    Center for Neurologic Diseases, Brigham and Women s Hospital, Boston, Massachusetts 02115, United States
    Biochemistry 51:3565-75. 2012
  16. pmc Drug resistance in breast cancer cells: biophysical characterization of and doxorubicin interactions with membrane lipids
    Chiranjeevi Peetla
    Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, United States
    Mol Pharm 7:2334-48. 2010
    ..MCF-7) and -resistant (MCF-7/ADR) breast cancer cells to characterize the biophysical properties of membrane lipids (particularly lipid packing and membrane fluidity) and to understand the role of the interaction of cell ..
  17. pmc Accumulation of raft lipids in T-cell plasma membrane domains engaged in TCR signalling
    Tobias Zech
    Sir William Dunn School of Pathology, University of Oxford, Oxford, UK
    EMBO J 28:466-76. 2009
    ..These results correlate the accumulation of specific molecular lipid species with the specific plasma membrane condensation at sites of TCR activation and with early TCR activation responses...
  18. pmc Membrane lipids of symbiotic algae are diagnostic of sensitivity to thermal bleaching in corals
    Dan Tchernov
    Environmental Biophysics and Molecular Ecology Program, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, 71 Dudley Road, New Brunswick, NJ 08901, USA
    Proc Natl Acad Sci U S A 101:13531-5. 2004
    ..A clocked molecular phylogenetic analysis suggests that the evolutionary history of symbiotic algae in cnidarians selected for a reduced tolerance to elevated temperatures in the latter portion of the Cenozoic...
  19. ncbi Mechanisms of membrane curvature sensing
    Bruno Antonny
    Université de Nice Sophia Antipolis and Centre National de la Recheche Scientifique, Institut de Pharmacologie Moleculaire et Cellulaire, 06560 Valbonne, France
    Annu Rev Biochem 80:101-23. 2011
    ..Membrane curvature sensors help to organize very diverse reactions, such as lipid transfer between membranes, the tethering of vesicles at the Golgi apparatus, and the assembly-disassembly cycle of protein coats...
  20. ncbi Membrane lipid domains distinct from cholesterol/sphingomyelin-rich rafts are involved in the ABCA1-mediated lipid secretory pathway
    A J Mendez
    University of Miami School of Medicine, Diabetes Research Institute, Miami, Florida 33101, USA
    J Biol Chem 276:3158-66. 2001
    ..We conclude that cholesterol and sphingomyelin-rich membrane rafts do not provide lipid for efflux promoted by apolipoproteins through the ABCA1-mediated lipid secretory pathway and that ABCA1 is not associated with these domains...
  21. pmc Lipid-protein interactions in double-layered two-dimensional AQP0 crystals
    Tamir Gonen
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 438:633-8. 2005
    ..We were therefore able to build an atomic model for the lipid bilayer surrounding the AQP0 tetramers, and we describe lipid-protein interactions...
  22. ncbi Why and how are peptide-lipid interactions utilized for self-defense? Magainins and tachyplesins as archetypes
    K Matsuzaki
    Graduate School of Biostudies, Kyoto University, Yoshida Shimoadachi Cho 46 29, Sakyo ku, Kyoto, Japan
    Biochim Biophys Acta 1462:1-10. 1999
  23. pmc The acyl-CoA synthetase encoded by LACS2 is essential for normal cuticle development in Arabidopsis
    Judy Schnurr
    Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164, USA
    Plant Cell 16:629-42. 2004
    ..The LACS2 gene and the corresponding lacs2 mutant will help in future studies of the cutin synthesis pathway and in understanding the consequences of reduced cutin production on many aspects of plant biology...
  24. pmc A permeable cuticle in Arabidopsis leads to a strong resistance to Botrytis cinerea
    Michael Bessire
    Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland
    EMBO J 26:2158-68. 2007
    ..Arabidopsis plants with a permeable cuticle have thus an altered perception of their environment and change their physiology accordingly...
  25. ncbi Functions of lipid rafts in biological membranes
    D A Brown
    Department of Biochemistry and Cell Biology, State University of New York at Stony Brook 11794 5215, USA
    Annu Rev Cell Dev Biol 14:111-36. 1998
    ..Rafts may also function in sorting and trafficking through the secretory and endocytic pathways...
  26. ncbi GPR55 and its interaction with membrane lipids: comparison with other endocannabinoid-binding receptors
    V Gasperi
    Department of Experimental Medicine and Surgery, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, Italy
    Curr Med Chem 20:64-78. 2013
    ..While the biological activity of eCBs and the influence of membrane lipids on their functions are rather well established, information regarding GPR55 is still scarce and often ..
  27. ncbi Membrane proteins diffuse as dynamic complexes with lipids
    Perttu S Niemelä
    VTT Technical Research Center of Finland, Espoo, Finland
    J Am Chem Soc 132:7574-5. 2010
    ..Our results indicate that, in studies of cell membranes, protein and lipid dynamics have to be considered together...
  28. pmc Mining the surface proteome of tomato (Solanum lycopersicum) fruit for proteins associated with cuticle biogenesis
    Trevor H Yeats
    Department of Plant Biology, Cornell University, Ithaca, NY 14853, USA
    J Exp Bot 61:3759-71. 2010
    ..This indicated a complex pattern of cuticle deposition, and models for cuticle biogenesis and restructuring are discussed...
  29. ncbi Mutants of Arabidopsis reveal many roles for membrane lipids
    James G Wallis
    Institute of Biological Chemistry, Washington State University, Pullman, WA 99164 6340, USA
    Prog Lipid Res 41:254-78. 2002
    ..Just as importantly, the availability of a series of plant lines with specific changes in membrane lipids have provided a new resource to study the structural and adaptive roles of lipids...
  30. pmc Phthiocerol dimycocerosates of M. tuberculosis participate in macrophage invasion by inducing changes in the organization of plasma membrane lipids
    Catherine Astarie-Dequeker
    CNRS, Institut de Pharmacologie et de Biologie Structurale IPBS, Toulouse, France
    PLoS Pathog 5:e1000289. 2009
    ..The DIM-induced changes in lipid ordering favour the efficiency of receptor-mediated phagocytosis of Mtb and contribute to the control of phagosomal pH driving bacilli in a protective niche...
  31. ncbi Bacterial membrane lipids: where do we stand?
    John E Cronan
    Department of Microbiology, University of Illinois, Urbana, Illinois 61801, USA
    Annu Rev Microbiol 57:203-24. 2003
    ..This review concentrates on the phospholipid synthetic pathway and discusses recent data on the synthesis and function of phospholipids mainly in the bacterium Escherichia coli...
  32. ncbi Amphipathic helices and membrane curvature
    Guillaume Drin
    Université de Nice Sophia Antipolis and CNRS, Institut de Pharmacologie Moleculaire et Cellulaire, Valbonne, France
    FEBS Lett 584:1840-7. 2010
    ..A more detailed description of the interaction between amphipathic helices and lipids is however needed; notably to explain how new helices contribute to detection of modest changes in curvature or even negative curvature...
  33. ncbi Lipid-protein interactions in biological membranes: a structural perspective
    A G Lee
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Bassett Crescent East, SO16 7PX, Southampton, UK
    Biochim Biophys Acta 1612:1-40. 2003
    ..The function of membrane proteins is dependent on the thickness of the surrounding lipid bilayer, sometimes on the presence of specific, usually anionic, phospholipids, and sometimes on the phase of the phospholipid...
  34. pmc The lipidomes of vesicular stomatitis virus, semliki forest virus, and the host plasma membrane analyzed by quantitative shotgun mass spectrometry
    Lucie Kalvodova
    Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    J Virol 83:7996-8003. 2009
  35. ncbi Mechanism and regulation of the violaxanthin cycle: the role of antenna proteins and membrane lipids
    Peter Jahns
    Plant Biochemistry, Heinrich Heine University Dusseldorf, Universitatsstr 1, D 40225 Dusseldorf, Germany
    Biochim Biophys Acta 1787:3-14. 2009
    ..The consequences of these findings for the mechanism and regulation of xanthophyll conversion in the thylakoid membrane will be discussed...
  36. ncbi Lysosomal degradation of membrane lipids
    Thomas Kolter
    LiMES Life and Medical Sciences Institute, Membrane Biology and Lipid Biochemistry Unit, c o Kekulé Institut für Organische Chemie und Biochemie, University of Bonn, Bonn, Germany
    FEBS Lett 584:1700-12. 2010
  37. pmc The acyltransferase GPAT5 is required for the synthesis of suberin in seed coat and root of Arabidopsis
    Fred Beisson
    Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824, USA
    Plant Cell 19:351-68. 2007
    ..These results provide evidence for a critical role of GPAT5 in polyester biogenesis in seed coats and roots and for the importance of lipid polymer structures in the normal function of these organs...
  38. ncbi Nanoscale analysis of supported lipid bilayers using atomic force microscopy
    Karim El Kirat
    Laboratoire de Biomécanique et Bioingénierie, UMR CNRS 6600, Universite de Technologie de Compiegne, BP 20529, F 60205 Compiègne Cedex, France
    Biochim Biophys Acta 1798:750-65. 2010
    ..Here, we survey recent progress made in the area...
  39. ncbi Polyunsaturated fatty acids in plasma and erythrocyte membrane lipids of diabetic children
    T Decsi
    Department of Paediatrics, University of Pecs, Pecs, Hungary
    Prostaglandins Leukot Essent Fatty Acids 67:203-10. 2002
    ..Fatty acids were determined by high-resolution capillary gas-liquid chromatography in plasma and erythrocyte membrane lipids in diabetic children (n = 40) and in age-matched healthy controls (n = 40)...
  40. ncbi Lipid II is an intrinsic component of the pore induced by nisin in bacterial membranes
    Eefjan Breukink
    Center of Biomembranes and Lipid Enzymology, Department of Biochemistry of Membranes, Institute for Biomembranes, University of Utrecht, Padualaan 8 3584 CH Utrecht, The Netherlands
    J Biol Chem 278:19898-903. 2003
    ..We show here that Lipid II is not only the receptor for nisin but an intrinsic component of the pore formed by nisin, and we present a new model for the pore complex that includes Lipid II...
  41. pmc Control of membrane lipid fluidity by molecular thermosensors
    Maria C Mansilla
    Instituto de Biología Molecular y Celular de Rosario IBR and Departamento de Microbiología, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Rosario, Argentina
    J Bacteriol 186:6681-8. 2004
  42. pmc Salt stress-induced changes in the transcriptome, compatible solutes, and membrane lipids in the facultatively phototrophic bacterium Rhodobacter sphaeroides
    Minoru Tsuzuki
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113 8657, Japan
    Appl Environ Microbiol 77:7551-9. 2011
    ..These findings set the stage for deciphering the salt stress-responsive regulatory network in R. sphaeroides...
  43. pmc Conditional peripheral membrane proteins: facing up to limited specificity
    Katarina Moravcevic
    Department of Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA
    Structure 20:15-27. 2012
    ..This broader range of recognition modes presents significant methodological challenges for a full structural understanding...
  44. pmc The eisosome core is composed of BAR domain proteins
    Agustina Olivera-Couto
    Institut Pasteur de Montevideo, Montevideo 11400, Uruguay
    Mol Biol Cell 22:2360-72. 2011
    ..Our results support a model in which BAR domain protein-mediated membrane bending leads to clustering of lipids and proteins within the plasma membrane...
  45. ncbi Folding and stability of alpha-helical integral membrane proteins
    Kevin R MacKenzie
    Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005, USA
    Chem Rev 106:1931-77. 2006
  46. ncbi Recent advances in structural research on ether lipids from archaea including comparative and physiological aspects
    Yosuke Koga
    Department of Chemistry, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan
    Biosci Biotechnol Biochem 69:2019-34. 2005
    ..In the last part of this review we discuss this problem...
  47. ncbi Elastic curvature constants of lipid monolayers and bilayers
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Dept Spektroskopie, 37070 Gottingen, Germany
    Chem Phys Lipids 144:146-59. 2006
    ..Consideration is given to the relation between the bending moduli of bilayers and the constituent monolayer leaflets. Useful mathematical relations involving the bending moduli and spontaneous curvature are summarized...
  48. ncbi The antitumor ether lipid ET-18-OCH(3) induces apoptosis through translocation and capping of Fas/CD95 into membrane rafts in human leukemic cells
    C Gajate
    , , CSIC-Universidad de Salamanca, Campus Miguel de Unamuno, Salamanca, Spain
    Blood 98:3860-3. 2001
    ..This translocation of Fas into membrane rafts may provide a mechanism for amplifying Fas signaling by reorganization of membrane microdomains...
  49. pmc Cuticular lipid composition, surface structure, and gene expression in Arabidopsis stem epidermis
    Mi Chung Suh
    Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824, USA
    Plant Physiol 139:1649-65. 2005
    ..The 15% transcripts preferentially expressed in the epidermis were enriched in genes encoding proteins predicted to be membrane associated and involved in lipid metabolism. An analysis of the lipid-related subset is presented...
  50. ncbi Protein modulation of lipids, and vice-versa, in membranes
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, Gottingen, Germany
    Biochim Biophys Acta 1778:1545-75. 2008
    This review describes: (i) perturbations of the membrane lipids that are induced by integral membrane proteins, and reciprocally, (ii) the effects that the lipids may have on the function of membrane-associated proteins...
  51. pmc Membrane organization and lipid rafts
    Kai Simons
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Cold Spring Harb Perspect Biol 3:a004697. 2011
    ..Here we will review the emerging principles of membrane architecture with special emphasis on lipid organization and domain formation...
  52. ncbi The role of membrane lipids in regulation of integrin functions
    G Pande
    Centre for Cellular and Molecular Biology, Uppal Road, 500 007, Hyderabad, India
    Curr Opin Cell Biol 12:569-74. 2000
    ..A better knowledge of integrin functions, especially in the lipid milieu of plasma membranes, is necessary for the understanding of the phenomena that are regulated by integrins...
  53. ncbi Membrane-associated stress proteins: more than simply chaperones
    Ibolya Horvath
    Institute of Biochemistry, Biological Research Centre, Szeged, Temesvari krt 62, Hungary
    Biochim Biophys Acta 1778:1653-64. 2008
    ..The possession of such information is critical if we are to target the membrane association principles of HSPs for successful drug development in most various diseases...
  54. ncbi The Arabidopsis DSO/ABCG11 transporter affects cutin metabolism in reproductive organs and suberin in roots
    David Panikashvili
    Department of Plant Sciences, Weizmann Institute of Science, PO Box 26, Rehovot 76100, Israel
    Mol Plant 3:563-75. 2010
    ..Taken together, this study provides new insights into cutin and suberin metabolism and their role in reproductive organs and roots development...
  55. ncbi Amino acid-containing membrane lipids in bacteria
    Otto Geiger
    Centro de Ciencias Genomicas, Universidad Nacional Autonoma de Mexico, Apdo Postal 565 A, Cuernavaca, Morelos CP62210, Mexico
    Prog Lipid Res 49:46-60. 2010
    In the bacterial model organism Escherichia coli only the three major membrane lipids phosphatidylethanolamine, phosphatidylglycerol, and cardiolipin occur, all of which belong to the glycerophospholipids...
  56. ncbi Lipid rafts as functional heterogeneity in cell membranes
    Daniel Lingwood
    Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany
    Biochem Soc Trans 37:955-60. 2009
    ..Evaluation of this property suggests that functional raft heterogeneity arises through principles of lipid-driven phase segregation coupled to additional chemical specificities, probably involving proteins...
  57. ncbi Membranes: a meeting point for lipids, proteins and therapies
    Pablo V Escriba
    Laboratory of Molecular Cell Biomedicine, Dept of Biology IUNICS, University of the Balearic Islands, Palma de Mallorca, Spain
    J Cell Mol Med 12:829-75. 2008
    ..b>Membrane lipids are crucial in the fission and fusion of lipid bilayers and they also act as sensors to control environmental ..
  58. pmc Role of lipids in virus replication
    Maier Lorizate
    Department of Infectious Diseases, Virology, University Heidelberg, D 69120 Heidelberg, Germany
    Cold Spring Harb Perspect Biol 3:a004820. 2011
    ..Here, we review viral interactions with cellular membranes important for virus entry, cytoplasmic genome replication, and virus egress...
  59. pmc Viral and developmental cell fusion mechanisms: conservation and divergence
    Amir Sapir
    Department of Biology, The Technion, Israel Institute of Technology, Haifa 32000, Israel
    Dev Cell 14:11-21. 2008
    ..These can serve as guidelines in the search for new fusogens, and may allow the formulation of a cross-species, unified theory to explain divergent and convergent evolutionary principles of membrane fusion...
  60. ncbi Isolation of an erythrocyte membrane protein that mediates Ca2+-dependent transbilayer movement of phospholipid
    F Basse
    Blood Research Institute of The Blood Center of Southeastern Wisconsin, Milwaukee, Wisconsin 53201, USA
    J Biol Chem 271:17205-10. 1996
  61. ncbi Effects of membrane lipids on ion channel structure and function
    Tommy S Tillman
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Cell Biochem Biophys 38:161-90. 2003
    ..The responsiveness of these ion channels to changes in the lipid environment illustrate how ion channels, and more generally, any membrane protein, may be regulated via cellular control of membrane composition...
  62. ncbi Role of cholesterol and lipid organization in disease
    Frederick R Maxfield
    Department of Biochemistry, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Nature 438:612-21. 2005
    b>Membrane lipids are essential for biological functions ranging from membrane trafficking to signal transduction...
  63. ncbi Role of membrane lipids in bacterial division-site selection
    Eugenia Mileykovskaya
    Department of Biochemistry and Molecular Biology, University of Texas Houston, Medical School, 6431 Fannin St, Suite 6 200, Houston, TX 77030, USA
    Curr Opin Microbiol 8:135-42. 2005
  64. ncbi Putative ammonia-oxidizing Crenarchaeota in suboxic waters of the Black Sea: a basin-wide ecological study using 16S ribosomal and functional genes and membrane lipids
    Marco J L Coolen
    Royal Netherlands Institute for Sea Research, Department of Marine Biogeochemistry and Toxicology, PO Box 59, 1790 AB Den Burg, The Netherlands
    Environ Microbiol 9:1001-16. 2007
    ..These results indicate an abundance of putative nitrifying Archaea at very low oxygen levels within the Black Sea and might form an important source of nitrite for the anammox reaction...
  65. ncbi Salt-induced changes in lipid composition and membrane fluidity of halophilic yeast-like melanized fungi
    Martina Turk
    Institute of Biochemistry, Medical Faculty, University of Ljubljana, Vrazov trg 2, 1000, Ljubljana, Slovenia
    Extremophiles 8:53-61. 2004
    ..pullulans and salt-sensitive S. cerevisiae, which is in good agreement with the lipid composition observed in this study...
  66. ncbi Molecular dynamics simulations of stratum corneum lipid models: fatty acids and cholesterol
    M Holtje
    Jülich Research Center, Germany
    Biochim Biophys Acta 1511:156-67. 2001
    ..The simulation results are in good agreement with our experimental data from FTIR analysis and NIR-FT Raman spectroscopy...
  67. ncbi Lipids on the move: simulations of membrane pores, domains, stalks and curves
    Siewert J Marrink
    Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands
    Biochim Biophys Acta 1788:149-68. 2009
  68. ncbi Toroidal pores formed by antimicrobial peptides show significant disorder
    Durba Sengupta
    Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Biochim Biophys Acta 1778:2308-17. 2008
    ..It was also found that in the absence of counter ions pores not only form more rapidly but lead to membrane rupture. The rupture process occurs via a novel recursive poration pathway, which we coin the Droste mechanism...
  69. ncbi Invadopodia biogenesis is regulated by caveolin-mediated modulation of membrane cholesterol levels
    Giusi Caldieri
    Tumor Cell Invasion Laboratory, Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, Chieti, Italy
    J Cell Mol Med 13:1728-40. 2009
  70. ncbi De novo lipogenesis protects cancer cells from free radicals and chemotherapeutics by promoting membrane lipid saturation
    Evelien Rysman
    Laboratory for Experimental Medicine and Endocrinology, K U Leuven, Leuven, Belgium
    Cancer Res 70:8117-26. 2010
    ..These findings provide important new insights into the role of de novo lipogenesis in cancer cells, and they provide a rationale for the use of lipogenesis inhibitors as antineoplastic agents and as chemotherapeutic sensitizers...
  71. ncbi Modulation of membrane curvature by phosphatidic acid and lysophosphatidic acid
    Edgar E Kooijman
    Department of Biochemistry of Membranes, Center for Biomembranes and Lipid Enzymology, Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
    Traffic 4:162-74. 2003
    ..Alternative models for the regulation of membrane fission based on the strong dependence of the molecular shape of (lyso)phosphatidic acid on pH and divalent cations are also discussed...
  72. pmc Characterization of an exported monoglyceride lipase from Mycobacterium tuberculosis possibly involved in the metabolism of host cell membrane lipids
    Karen Côtes
    Laboratoire d Enzymologie Interfaciale et de Physiologie de la Lipolyse, UPR 9025 CNRS, 31 Chemin Joseph Aiguier, F 13402 Marseille Cedex 20, France
    Biochem J 408:417-27. 2007
    ..tuberculosis. Our results identify Rv0183 as the first exported lipolytic enzyme to be characterized in M. tuberculosis and suggest that Rv0183 may be involved in the degradation of the host cell lipids...
  73. pmc Phospholipid synthesis and lipid composition of subcellular membranes in the unicellular eukaryote Saccharomyces cerevisiae
    E Zinser
    Institut fur Biochemie und Lebensmittelchemie, Technische Universitat Graz, Austria
    J Bacteriol 173:2026-34. 1991
    ..The rigidity of the plasma membrane and of secretory vesicles, determined by measuring fluorescence anisotropy by using trimethylammonium diphenylhexatriene as a probe, can be attributed to the high content of ergosterol...
  74. pmc Dynamic, yet structured: The cell membrane three decades after the Singer-Nicolson model
    G Vereb
    Department of Biophysics and Cell Biology, Research Center for Molecular Medicine, Medical and Health Science Center, University of Debrecen, H 4012, Debrecen, Hungary
    Proc Natl Acad Sci U S A 100:8053-8. 2003
  75. ncbi Membrane proteins, lipids and detergents: not just a soap opera
    Annela M Seddon
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Biochim Biophys Acta 1666:105-17. 2004
    ..We highlight some of the lessons learnt from studies of membrane protein folding in vitro and give an overview of the role that lipids can play in stabilizing the proteins...
  76. pmc Membrane binding domains
    James H Hurley
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD 20892, USA
    Biochim Biophys Acta 1761:805-11. 2006
    ..These domains use a combination of specific headgroup interactions, hydrophobic membrane penetration, electrostatic surface interactions, and shape complementarity to bind to specific subcellular membranes...
  77. pmc Membrane potential governs lateral segregation of plasma membrane proteins and lipids in yeast
    Guido Grossmann
    University of Regensburg, Cell Biology and Plant Physiology, Regensburg, Germany
    EMBO J 26:1-8. 2007
    ..In accordance with these observations, depolarized membranes also considerably change their physical properties (detergent sensitivity)...
  78. pmc Cytochrome P450 family member CYP704B2 catalyzes the {omega}-hydroxylation of fatty acids and is required for anther cutin biosynthesis and pollen exine formation in rice
    Hui Li
    School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China
    Plant Cell 22:173-90. 2010
  79. ncbi Two types of Alzheimer's beta-amyloid (1-40) peptide membrane interactions: aggregation preventing transmembrane anchoring versus accelerated surface fibril formation
    Marcus Bokvist
    Department of Biophysical Chemistry, Umea University, 90187 Umea, Sweden
    J Mol Biol 335:1039-49. 2004
  80. ncbi Lipid traffic: floppy drives and a superhighway
    Joost C M Holthuis
    Department of Membrane Enzymology, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Nat Rev Mol Cell Biol 6:209-20. 2005
    Understanding how membrane lipids achieve their non-random distribution in cells is a key challenge in cell biology at present...
  81. ncbi How curved membranes recruit amphipathic helices and protein anchoring motifs
    Nikos S Hatzakis
    Bio Nanotechnology Laboratory, Department of Neuroscience and Pharmacology and Nano Science Center, University of Copenhagen, Copenhagen, Denmark
    Nat Chem Biol 5:835-41. 2009
    ..We therefore anticipate that MC will promote the redistribution of proteins that are anchored in membranes through other types of hydrophobic moieties...
  82. pmc Influenza virus M2 protein mediates ESCRT-independent membrane scission
    Jeremy S Rossman
    Department of Biochemistry, Northwestern University, Evanston, IL 60208, USA
    Cell 142:902-13. 2010
    ..These data suggest that M2 mediates the final steps of budding for influenza viruses, bypassing the need for host ESCRT proteins...
  83. pmc Spatial segregation of gamma-secretase and substrates in distinct membrane domains
    Kulandaivelu S Vetrivel
    Department of Neurobiology, Pharmacology, and Physiology, The University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 280:25892-900. 2005
  84. ncbi Crenarchaeol dominates the membrane lipids of Candidatus Nitrososphaera gargensis, a thermophilic group I.1b Archaeon
    Angela Pitcher
    Department of Marine Organic Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, Den Burg, The Netherlands
    ISME J 4:542-52. 2010
    Analyses of archaeal membrane lipids are increasingly being included in ecological studies as a comparatively unbiased complement to gene-based microbiological approaches...
  85. pmc Fast molecular tracking maps nanoscale dynamics of plasma membrane lipids
    Steffen J Sahl
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 107:6829-34. 2010
    ..Our experimental approach demonstrates that fast molecular movements can be tracked with minimal invasion, which can reveal new important details of cellular nano-organization...
  86. ncbi Effect of line tension on the lateral organization of lipid membranes
    Ana J García-Sáez
    Biotechnologisches Zentrum der Techische Universität Dresden, 1307 Dresden, Germany
    J Biol Chem 282:33537-44. 2007
    ..Our experiments contribute significantly to our knowledge of the physical-chemical parameters that control membrane organization. Importantly, the general trends observed can be extended to cellular membranes...
  87. ncbi Caveolins and cellular cholesterol balance
    E Ikonen
    Department of Biochemistry, National Public Health Institute, Mannerheimintie 166, 00300 Helsinki, Finland
    Traffic 1:212-7. 2000
    ..Here we review evidence for caveolin regulation of cholesterol transport and consider how this may relate to signal transduction...
  88. ncbi Intact polar membrane lipids in prokaryotes and sediments deciphered by high-performance liquid chromatography/electrospray ionization multistage mass spectrometry--new biomarkers for biogeochemistry and microbial ecology
    Helen F Sturt
    Woods Hole Oceanographic Institution, Department of Geology and Geophysics, Woods Hole, MA 02543, USA
    Rapid Commun Mass Spectrom 18:617-28. 2004
    ..However, when still intact, i.e., as glycerol esters and ethers with attached polar headgroups, membrane lipids are diagnostic for living prokaryotes, which makes them excellent biomarkers for the study of in situ ..
  89. ncbi The lipid droplet binding domain of hepatitis C virus core protein is a major determinant for efficient virus assembly
    Anna Shavinskaya
    Department of Molecular Virology, University of Heidelberg, 69120 Heidelberg, Germany
    J Biol Chem 282:37158-69. 2007
    ..Taken together, our results demonstrate that the binding strength of the D2 domain of core for LDs is crucial for determining the efficiency of virus assembly...
  90. pmc T cell receptor ligation induces the formation of dynamically regulated signaling assemblies
    Stephen C Bunnell
    Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 158:1263-75. 2002
  91. pmc In vitro low-level resistance to azoles in Candida albicans is associated with changes in membrane lipid fluidity and asymmetry
    Avmeet Kohli
    Membrane Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India
    Antimicrob Agents Chemother 46:1046-52. 2002
    ..The results of the present study suggest for the first time that changes in the status of membrane lipid phase and asymmetry could contribute to azole resistance in C. albicans...
  92. pmc Membrane sphingolipid-ergosterol interactions are important determinants of multidrug resistance in Candida albicans
    Kasturi Mukhopadhyay
    Membrane Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067 Centre for Cellular and Molecular Biology, Hyderabad 500007, India
    Antimicrob Agents Chemother 48:1778-87. 2004
    ..albicans cells...
  93. ncbi Biophysical properties of lipids and dynamic membranes
    P A Janmey
    Institute for Medicine and Engineering, Department of Physiology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Trends Cell Biol 16:538-46. 2006
    ..Here, we highlight some recent reports that the physical properties of the membrane can help control the function of transmembrane proteins and the motor-dependent elongation of internal organelles, such as the endoplasmic reticulum...
  94. ncbi Molecular mechanisms of membrane perturbation by antimicrobial peptides and the use of biophysical studies in the design of novel peptide antibiotics
    K Lohner
    Institute of Biophysics and X ray Structure Research, Austrian Academy of Sciences, A 8042 Graz, Austria
    Comb Chem High Throughput Screen 8:241-56. 2005
    ..Emphasis is on the role of membrane lipid composition in these processes and in the translocation of antimicrobial peptides...
  95. ncbi Comparative lipidomic profiling of xylose-metabolizing S. cerevisiae and its parental strain in different media reveals correlations between membrane lipids and fermentation capacity
    Jinmei Xia
    PO Box 6888, Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, PR China
    Biotechnol Bioeng 108:12-21. 2011
    ..Interestingly, PI synthesis lagged behind when available nutrients were limited, and PI levels were closely correlated with xylose metabolism...
  96. ncbi Ageing-related tissue-specific alterations in mitochondrial composition and function are modulated by dietary fat type in the rat
    Jose L Quiles
    Department of Physiology, Institute of Nutrition and Food Technology, University of Granada, C Ramon y Cajal 4, 18071 Granada, Spain
    J Bioenerg Biomembr 34:517-24. 2002
    ..Since high levels of cytochrome b have been related to increased age, it could be hypothesized that olive oil could lead to less aged mitochondria...
  97. ncbi Membrane integration of a mitochondrial signal-anchored protein does not require additional proteinaceous factors
    Elisa Merklinger
    Interfaculty Institute of Biochemistry, University of Tubingen, Hoppe Seyler Strasse 4, Tubingen, Germany
    Biochem J 442:381-9. 2012
    ..Collectively, our results suggest that SA proteins are specifically inserted into the MOM by a process that is not dependent on additional proteins, but is rather facilitated by the distinct lipid composition of this membrane...
  98. pmc Distribution of membrane lipids of planktonic Crenarchaeota in the Arabian Sea
    Jaap S Sinninghe Damsté
    Department of Marine Biogeochemistry and Toxicology, Royal Netherlands Institute for Sea Research, Den Burg, Texel, The Netherlands
    Appl Environ Microbiol 68:2997-3002. 2002
    Intact core tetraether membrane lipids of marine planktonic Crenarchaeota were quantified in water column-suspended particulate matter obtained from four depth intervals ( approximately 70, 500, 1,000 and 1,500 m) at seven stations in ..
  99. ncbi Effect of aliphatic alcohols on growth and degree of saturation of membrane lipids in Acinetobacter calcoaceticus
    Nadja Kabelitz
    Department of Remediation Research, Centre for Environmental Research UFZ Leipzig Halle, Permoserstr 15, 04318 Leipzig, Germany
    FEMS Microbiol Lett 220:223-7. 2003
  100. pmc In-depth profiling of the LiaR response of Bacillus subtilis
    Diana Wolf
    Department of Biology I, Ludwig Maximilians University of Munich, Grosshaderner Str 2 4, D 82152 Planegg Martinsried, Germany
    J Bacteriol 192:4680-93. 2010
    ..Our data suggest that the LiaFSR system of B. subtilis and, presumably, other Firmicutes bacilli coordinates a phage shock protein-like response...
  101. pmc A distinct type of glycerol-3-phosphate acyltransferase with sn-2 preference and phosphatase activity producing 2-monoacylglycerol
    Weili Yang
    Department of Plant Biology, Michigan State University, East Lansing, MI 48824, USA
    Proc Natl Acad Sci U S A 107:12040-5. 2010
    ..These results provide insight into the biosynthetic assembly of cutin and suberin, the two most abundant glycerolipid polymers in nature...

Research Grants67

  1. Mapping for Specialized Domains for FCeRI Signaling &Internalization
    Bridget S Wilson; Fiscal Year: 2013
    ..In Aim 1, we will develop new monovalent probes for plasma membrane lipids. We will use both transmission and X- ray spectral electron microscopy to map the distributions of tagged ..
    ..that 4-hydroxynonenal (HNE), an aldehyde generated by the action of reactive oxygen species (ROS) on membrane lipids, is an endogenous agonist of TRPA1 which causes peptide release, neurogenic inflammation and pain...
  3. Inflammatory hyperalgesia due to TRPV1, the pepper spray receptor in the cornea
    Sharona E Gordon; Fiscal Year: 2013
    ..Although cell surface receptor-stimulated trafficking of membrane lipids and membrane proteins is of broad significance to biology, the molecular mechanisms by which it occurs are ..
  4. Skeletal Muscle Mitochondrial Adaptations to a Two-Year Caloric Restiction
    STEVEN RICHARD SMITH; Fiscal Year: 2012
    ..As mitochondrial ROS can cause damage to mitochondrial DNA, proteins, and membrane lipids, a self-perpetuating and destructive cycle can ensue in which increased ROS production leads to incremental ..
  5. Phosphoprotein CDP138 regulates glucose metabolism
    ZHEN YUE JIANG; Fiscal Year: 2013
    ..CDP138 forms oligomers and is capable of binding calcium and membrane lipids. We demonstrated that both the C2 domain and Akt phosphorylation site Ser197 in CDP138 are critical for ..
  6. Label-Free Chemical Imaging for Biological Applications
    Wei Min; Fiscal Year: 2012
    ..electric field across the plasma membranes during action potentials should shift the vibrational frequency of membrane lipids, and we plan to employ this vibrational electrochromism as a label-free contrast mechanism for voltage ..
  7. Roles of Phospholipase D in AT1 Receptor Endocytosis
    Guangwei Du; Fiscal Year: 2010
    ..roles for many proteins in AT1R trafficking are becoming clear, less is known about the specific functions of membrane lipids. It has been recognized that interaction between proteins and membrane (particularly phospholipids) drives ..
  8. Receptor-mediated Endocytosis-Mechanism and Function
    Frederick R Maxfield; Fiscal Year: 2013
    ..These two themes interact synergistically because proteins are responsible for lipid transport, and membrane lipids strongly Influence the transport and distribution of membrane proteins...
    Michael F Brown; Fiscal Year: 2012
    ..Here 2H and 31P NMR will investigate the biophysical properties of membrane lipids that influence the photochemical function of rhodopsin...
  10. Sphingolipid Metabolism and Signaling in the Retina
    Md Nawajes A Mandal; Fiscal Year: 2013
    Project Summary/Abstract: Sphingolipids are a family of membrane lipids with important structural roles in the regulation of the fluidity and subdomain structure of the lipid bilayer, especially lipid rafts...
  11. Genetic Approaches to Lipid Metabolism
    John E Cronan; Fiscal Year: 2013
    ..Since fatty acids are an essential component of bacterial membrane lipids, several of these enzymes are the targets of antimicrobials...
    Bertil Hille; Fiscal Year: 2012
    ..proposal concerns the regulation of ion-channel function by G-protein-coupled receptor (GPCR) signaling to membrane lipids. It focuses on the hypothesis that the function of many ion channels depends on the concentration of one rare ..
  13. Soy sphingadienes and related compounds in colon cancer chemoprevention and treat
    Julie D Saba; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Sphingolipids are conserved membrane lipids that regulate cell proliferation, apoptosis and angiogenesis...
  14. Commensal Bacteroides signal through sphingolipid rafts during intestinal stress
    Dennis L Kasper; Fiscal Year: 2012
    ..fragilis membrane lipids considerably changes the physical organization of these lipids with the appearance of large sphingolipid- ..
  15. Longitudinal study of membrane lipids: pre-clinical Alzheimer's biomarkers
    Michelle M Mielke; Fiscal Year: 2010
    ..AD is a progressive neurodegenerative disorder and membrane lipids and lipid metabolites are involved in the neurodegeneration...
    Samuel I Miller; Fiscal Year: 2013
    ..This grant will also study the mechanisms by which the PhoP/Q regulated remodeling of membrane lipids occurs and its role in bacterial virulence and the structure...
  17. STAT3 Functional Defects and a Novel Therapeutic Approach for Hyper IgE Syndrome
    Hans Ochs; Fiscal Year: 2009
    ..PH domains are known to bind to membrane lipids (e.g., PIP2) and thus are responsible for localizing PH domain containing proteins to the cell membrane...
  18. Vasodilation via selective pharmacological targeting of BK channel beta1 subunits
    Alex M Dopico; Fiscal Year: 2013
    ..activate BK via specific docking on the channel or, rather, secondarily to nonspecific perturbation of membrane lipids. We recently found that cholane steroids, such as lithocholate (LC), selectively activate 21-containing BK, ..
  19. Understanding the role of myosin in endocytosis
    ERIC BRECHER LEWELLYN; Fiscal Year: 2013
    ..endocytosis is a conserved pathway in eukaryotic cells for internalizing external macromolecules, membrane lipids, and cell surface receptors...
  20. Exosites in Matrix Metalloproteinase Localization and Activity
    Steven R Van Doren; Fiscal Year: 2013
    ..hypotheses of various exosites that support (1) affinity for and activity upon elastin, (2) association with membrane lipids, and (3) association with and activation by sulfated saccharides...
    Jack H Freed; Fiscal Year: 2013
    ..functional changes, and in parallel experiments observe the changes occurring in the dynamic structure of the membrane lipids. This ability to accurately characterize both membrane host and membrane protein is an innovative approach in ..
  22. Transcriptional regulation of lipid homeostasis in mycobacteria
    Hugo Gramajo; Fiscal Year: 2013
    ..this proposal is to understand how mycobacteria exert this exquisite control over the biosynthesis of their membrane lipids and find out the key components of the regulatory network that control fatty acid and mycolic acid ..
  23. Biochemistry of lysyl-tRNA synthetases
    Michael Ibba; Fiscal Year: 2013
    ..the canonical lysyl-tRNA synthetase provides substrates both to translate lysine codons and to modify membrane lipids, while the closely related paralog PoxA instead participates in the post-translational modification of ..
  24. Defining the Impact of Lipid Synthesis and Turnover on Aging in C. elegans
    CARISSA OLSEN; Fiscal Year: 2013, because they require high levels of isotope enrichment to obtain the resolution required to analyze membrane lipids in detail. In the Van Gilst lab, I developed stable isotope enrichment strategies in C...
  25. Yeast MAPK Signaling Networks: Specificity, Regulation and Function
    Jeremy W Thorner; Fiscal Year: 2013
    ..distribution;(3) genetic and biochemical studies of the control of the remodeling of other plasma membrane lipids in pheromone- and nutrient limitation-induced polarized growth;(4) biochemical and genetic analysis of the ..
  26. Endocytic and Exocytic Traffic in Bladder uroepithelium
    Gerard L Apodaca; Fiscal Year: 2013
    ..the membrane content of surface receptors and channels, other structural proteins such as the uroplakins, and membrane lipids. Furthermore, alterations in membrane traffic are likely to promote the invasion and release of uropathogenic ..
    Pietro De Camilli; Fiscal Year: 2009
    ..The project will contribute to expand our knowledge on the role of membrane lipids as key regulatory molecules in brain, an area of research which is rapidly expanding...
  28. Regulation of Mycobacterium tuberculosis proliferation
    Murty Madiraju; Fiscal Year: 2009 be regulated during intracellular maintains an active replicative state with increase in bacterial burden, membrane lipids, possibly modulated by LysX, a hitherto unrecognized Z replication and or the factors that in part, on its ..
  29. Trasnducin Interactions with Photoreceptor Membranes
    Theodore G Wensel; Fiscal Year: 2013
    ..time scale at the surface of disk membranes, and depend critically on the environment provided by the membrane lipids, so that they must be studied in that context...
  30. TRP and AQP Channels: Modulation of Function, Raft Location by Membrane Lipids
    THOMAS J MC INTOSH; Fiscal Year: 2012
    ..are fundamental issues because the functions of many ion channels have been shown to be modified by specific membrane lipids such as phosphatidylinositol-4,5 bisphosphate (PIP2), as well as by membrane material properties controlled ..
  31. Membranes in the Initiation of Chromsomal Replication
    Elliott Crooke; Fiscal Year: 2010
    ..This work should provide insight into the interactions between membrane lipids and DNA synthesis components, and the functions that membranes may play in cell-cycle and growth controlled ..
  32. Drug Resistance in Cancer Therapy
    VINOD D LABHASETWAR; Fiscal Year: 2013
    ..of the surface-modifying agent at the NP interface influences the biophysical interactions of NPs with cell-membrane lipids, which then affect the cellular delivery of the encapsulated therapeutics and tumor targeting in vivo...
  33. Glycolipid transfer regulation by membrane interfaces
    Rhoderick E Brown; Fiscal Year: 2013
    ..the means to quantita- tively assess lateral elastic interactions of GSLs with each other and with other membrane lipids while achieving nanoscale resolution into lipid lateral mixing, enabling fundamental insights into the ..
  34. Phospholipid dynamics and lipolysis in membrane models
    Joseph Hajdu; Fiscal Year: 2013
    ..Elucidation of phospholipid structure and dynamics in membranes and the way they interact with other membrane lipids and proteins will provide important information toward better understanding membrane behavior in health and ..
  35. Quantitative proteomic profiling of reactive cysteines in oxidative stress
    Chu Wang; Fiscal Year: 2012
    ..inflammation and exposure to environmental pollutants, such as nitrogen dioxide or ozone, peroxidation of membrane lipids yields several reactive end products, among which 4-hydroxy-2-nonenal (HNE) is a major species...
  36. Biopolymer Surfactants for Sealing Electroporated Membranes
    RAPHAEL CARL LEE; Fiscal Year: 2010 altering interfacial water structure, thus reducing membrane tension, which then permits reorganization of membrane lipids into a lipid bilayer...
  37. Small Molecule Receptors for Membrane Lipids
    Jianmin Gao; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Small Molecule Receptors for Membrane Lipids Project Summary Cell membranes consist of a variety of lipids. It is clear that they do not simply serve as a physical barrier of a cell...
  38. Kindlin-2 in Cell-Matrix Adhesion and Signaling
    Chuanyue Wu; Fiscal Year: 2013
    ..project periods, the applicant hypothesizes that kindlin-2 regulates these processes through interacting with membrane lipids and protein components of cell-ECM adhesions...
  39. Structural basis for HIV-1 Gag interactions with cellular constituents
    JAMIL SUBHI SAAD; Fiscal Year: 2013
    ..Gag targeting and assembly on the PM proceed via a dual engagement of the MA domain with PI(4,5)P2 and major membrane lipids. A number of cellular proteins have been proposed to facilitate Gag intracellular trafficking and targeting ..
  40. Structure and Function in Membrane Proteins
    William Dowhan; Fiscal Year: 2013
    ..From these studies a set of rules is evolving in which short-term cooperative charge interactions between membrane lipids and proteins coupled with long-term interactions between transmembrane domains are important determinants of ..
  41. Lipid acceleration of transthyretin amyloidosis
    Irit Rappley; Fiscal Year: 2012
    ..Hypothesis: The central hypothesis of this proposal is that certain membrane lipids alter the kinetics and pathway of TTR aggregation through distinct mechanisms, resulting in discrete ..
  42. The budding yeast endocytic pathway
    David G Drubin; Fiscal Year: 2013
    ..2) The roles of specific plasma membrane lipids and protein-lipid interactions during each stage of the CME pathway will be elucidated...
  43. Anachem Lipidic Cubic Phase Crystallization Robot
    Robert M Stroud; Fiscal Year: 2010
    ..This robot will allow a different type of crystallization of membrane proteins in membrane lipids. The method has been used with landmark success by the Stevens group at Scripps, using Caffrey's robot, ..
  44. Interaction between Alzheimer's beta-Amyloid Peptide and Phospholipid Bilayers
    Miroslav Peric; Fiscal Year: 2013
    ..that the pathophysiological effects of amyloid plaques may partially be caused by direct interactions between membrane lipids and ?-amyloid peptides, the molecular basis of the interaction is still not well understood...
  45. The influence of beta-subunits and sphingomyelin on sodium channel function
    FRANK GERT WERNER BOSMANS; Fiscal Year: 2013 independent units, Nav channels are part of a signaling complex that involves auxiliary proteins and membrane lipids. My goal is to address fundamental questions on the identities of the Nav channel signaling complex ..
  46. Chlamydia co-option of Eukaryotic Lipid Droplets
    Raphael H Valdivia; Fiscal Year: 2010
    ..In infected cells, Chlamydia efficiently acquires host-derived membrane lipids despite an apparent isolation of the bacterial - containing vacuole ("inclusion") from classical endocytic ..
  47. Control of Retrovirus Assembly by Membrane Lipid Thermodynamic Activity.
    ..2) How is the thermodynamic activity of Gag's other binding partner, PI(4,5)P2 controlled by the other membrane lipids, and in particular, what factors control the formation of PI(4,5)P2 domains? Are these domains the sites of ..
  48. The Mechanisms of Lipid Droplet Formation and Regulation
    Robert V Farese; Fiscal Year: 2013
    ..and store cellular lipids, including triacylglycerols (TGs), which provide stores of energy and sources of membrane lipids. Despite recent advances in understanding LD biology in cellular physiology and disease, most fundamental key ..
    NAZZARENO D'AVANZO; Fiscal Year: 2012
    ..homologues are limited since they share only ~30% sequence identity and are regulated differently by some membrane lipids. Thus, until recently, biochemical and structural analyses on eukaryotic Kir channels remained elusive...
  50. Structure-function studies of single transmembrane helix receptors
    STEVEN OWEN SMITH; Fiscal Year: 2011
    ..The proposed studies on the JM regions of these receptors address questions involving the role of membrane lipids, such as the phosphoinositides, in regulating receptor activity...
    Michael F Brown; Fiscal Year: 2010
    ..Biophysical methods will characterize the influences of membrane lipids on the Meta I}Meta II transition of rhodopsin...
  52. Presenilin Biology and the Mechanisms of Alzheimer's Disease
    RUDOLPH EMILE TANZI; Fiscal Year: 2013
    ..mechanism of coordinated alpha-, beta- and gamma-secretase processing? How do certain synaptic proteins and membrane lipids regulate PS activity in neurons, affecting the crucial A(l42/4o ratio? Can one design drugs that are ..
  53. Structure and Function of Membrane Protein Complexes by Solid State NMR
    CHAD RIENSTRA; Fiscal Year: 2009
    ..The enzyme was uniformly labeled with 13C and 15N, then purified and pelleted with endogenous membrane lipids. Excellent spectra can be obtained with approximately 50 nmol (approximately 8 mg) of purified protein (using ..
  54. Physiological Significance of Conjugated Double Bonds in Membrane Lipids
    Papasani Subbaiah; Fiscal Year: 2009
    Sahebarao Mahadik; Fiscal Year: 2002
    ..Effects of oxidative cell injury will be examined in lymphocyte plasma membrane lipids, mitochondrial membranes, cellular proteins, and DNA (both Mitochondrial and nuclear)...
    Julie Andersen; Fiscal Year: 1999 converted by reaction with free iron to reactive hydroxyl radicals which can degrade DNA, proteins, and membrane lipids resulting in cellular degeneration...
  57. Refinement of LC/ESR/MS for Studying Lipid Free Radicals in Colon Cancer Growth
    Steven Qian; Fiscal Year: 2009
    ..g. lipoxygenase (15-LOXs) and cyclooxygenase (COXs), that metabolize dietary and membrane lipids (polyunsaturated fatty acids, or PUFAs) are particularly involved in colon cancer progression...
    Edward Block; Fiscal Year: 1993 to test the hypothesis that high partial pressures of O2, which can cause peroxidative cleavage of membrane lipids, alter the fluidity of the lipid bilayer of the plasma membrane of pulmonary endothelial cells, and these ..
    Raj Gupta; Fiscal Year: 2006
    ..We seek to understand how the regulation of intracellular concentrations of essential metal ions and membrane lipids goes astray in health disorders, especially hypertension and diabetes...
    Robert Fowler; Fiscal Year: 1991
    ..species of oxygen are normally generated by respiring cells and are known to be toxic, damaging proteins, membrane lipids and DNA...
    STEPHEN SHOHET; Fiscal Year: 2000
    ..of spectrin, weaken distance-dependent interactions between the skeleton and the bilayer, and destabilize membrane lipids. To test this hypothesis, Dr. Shohet will pursue the following specific aims: 1...
    Minor Coon; Fiscal Year: 2000
    ..chronic exposure of animals to ethanol, by causing induction of P450s, leads to enhanced degradation of membrane lipids and eventual loss of membrane integrity...
  63. Prostate MRI and MRS: Correlations with Gene Expression
    Sandra Gaston; Fiscal Year: 2007
    ..kinase (the first and probably regulatory enzyme in the pathway for de novo synthesis of choline containing membrane lipids) in tissue-print micro-peels obtained from radical prostatectomy specimens containing Gleason grade 6,7 or 8 ..
  64. Membrane Targeting by Phosphoinositide Binding Proteins
    Wonhwa Cho; Fiscal Year: 2013 applicant): It has become increasingly difficult to find an area of biology and medicine in which membrane lipids do not play important signaling and regulatory roles...
    THOMAS SANTORO; Fiscal Year: 1992
    Chaim Hershko; Fiscal Year: 1991
    ..heart cells results in abnormal contractility and electrophysiologic behaviour and increased peroxidation of membrane lipids, reversible by deferoxamine treatment...
    JANNE CANNON; Fiscal Year: 1992
    ..However, the precise role of these components and how they interact with one another, membrane lipids, and the signal peptide and mature moiety of precursor proteins to facilitate the export process have yet to ..