Genomes and Genes
Summary: The lipopolysaccharide-protein somatic antigens, usually from gram-negative bacteria, important in the serological classification of enteric bacilli. The O-specific chains determine the specificity of the O antigens of a given serotype. O antigens are the immunodominant part of the lipopolysaccharide molecule in the intact bacterial cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Publications315 found, 100 shown here
- Biosynthesis and assembly of capsular polysaccharides in Escherichia coliChris Whitfield
Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada
Annu Rev Biochem 75:39-68. 2006..These systems have an impact on both a general understanding of membrane trafficking in bacteria and on bacterial pathogenesis...
- Biosynthesis of O-antigens: genes and pathways involved in nucleotide sugar precursor synthesis and O-antigen assemblyGabrielle Samuel
School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW 2006, Australia
Carbohydr Res 338:2503-19. 2003..This review is a summary of the current knowledge on these three groups of genes that comprise the O-antigen gene clusters, focusing on the most extensively studied E. coli and S. enterica gene clusters...
- Bacterial polysaccharide synthesis and gene nomenclatureP R Reeves
Dept of Microbiology, University of Sydney, NSW, Australia
Trends Microbiol 4:495-503. 1996..We propose a scheme applicable to all species that distinguishes different classes of genes, provides a single name for all genes of a given function and greatly facilitates comparative studies...
- Structure, assembly and regulation of expression of capsules in Escherichia coliC Whitfield
Department of Microbiology, University of Guelph, Ontario, Canada
Mol Microbiol 31:1307-19. 1999..coli into four distinct groups...
- Regulation of Salmonella typhimurium lipopolysaccharide O antigen chain length is required for virulence; identification of FepE as a second WzzGerald L Murray
Department of Molecular Biosciences, The University of Adelaide, Adelaide, South Australia, Australia, 5005
Mol Microbiol 47:1395-406. 2003..The presence of wzzfepE homologues in the genomic sequences of strains of Escherichia coli, Shigella flexneri and multiple serovars of Salmonella suggests that bimodality of LPS Oag is a common phenomenon in the Enterobacteriaceae...
- Structure and genetics of Shigella O antigensBin Liu
TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China
FEMS Microbiol Rev 32:627-53. 2008This review covers the O antigens of the 46 serotypes of Shigella, but those of most Shigella flexneri are variants of one basic structure, leaving 34 Shigella distinct O antigens to review, together with their gene clusters...
- Detection of O antigens in Escherichia coliChitrita Debroy
E coli Reference Center, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, USA
Anim Health Res Rev 12:169-85. 2011Lipopolysaccharide on the surface of Escherichia coli constitutes the O antigens which are important virulence factors that are targets of both the innate and adaptive immune systems and play a major role in host-pathogen interactions...
- Regulation by a novel protein of the bimodal distribution of lipopolysaccharide in the outer membrane of Escherichia coliR A Batchelor
Department of Microbiology and Immunology, Baylor College of Medicine, Texas Medical Center, Houston 77030
J Bacteriol 173:5699-704. 1991..Mutation of the gene encoding this protein destroyed its ability to regulate O-chain distribution. We propose to call this regulator gene rol, for regulator of O length...
- Altering the length of the lipopolysaccharide O antigen has an impact on the interaction of Salmonella enterica serovar Typhimurium with macrophages and complementGerald L Murray
Australian Bacterial Pathogenesis Program, Discipline of Microbiology and Immunology, School of Molecular and Biomedical Sciences, The University of Adelaide, Adelaide, Australia 5005
J Bacteriol 188:2735-9. 2006..No evidence was found to suggest that modifying the length of the O-antigen polymer affected expression of the O1, O4, or O5 antigenic factors...
- An O-antigen processing function for Wzx (RfbX): a promising candidate for O-unit flippaseD Liu
Department of Microbiology, University of Sydney, New South Wales, Australia
J Bacteriol 178:2102-7. 1996....
- The structures of Escherichia coli O-polysaccharide antigensRoland Stenutz
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Stockholm, Sweden
FEMS Microbiol Rev 30:382-403. 2006..In addition, results pertinent to the biosynthesis of the repeating units of O-antigens are discussed. The ECODAB is accessible to the scientific community at http://www.casper.organ.su.se/ECODAB/...
- Molecular analysis of the contribution of the capsular polysaccharide and the lipopolysaccharide O side chain to the virulence of Klebsiella pneumoniae in a murine model of pneumoniaGuadalupe Cortés
Unidad de Investigacion, Hospital Universitario Son Dureta, Palma de Mallorca, Spain
Infect Immun 70:2583-90. 2002..We conclude that CPS, but not LPS O side chain, is essential for Klebsiella pneumonia because it modulates the deposition of C3 and protects the microorganisms against human AM phagocytosis...
- O-antigen structural variation: mechanisms and possible roles in animal/plant-microbe interactionsInge Lerouge
Centre of Microbial and Plant Genetics, Katholieke Universtiteit Leuven, Kasteelpark Arenberg 20, B 3001, Heverlee, Belgium
FEMS Microbiol Rev 26:17-47. 2002..However, the genetic mechanisms by which the LPS epitope changes are regulated remain unclear. Finally, the possible roles of O-antigen variations in symbiosis will be discussed...
- Genetic modulation of Shigella flexneri 2a lipopolysaccharide O antigen modal chain length reveals that it has been optimized for virulenceRenato Morona
Department of Molecular Biosciences, University of Adelaide, Adelaide, South Australia, Australia, 5005
Microbiology 149:925-39. 2003..It is suggested that S. flexneri 2a has evolved to synthesize LPS with two Oag modal chain lengths, as S-type Oag chains allow IcsA to function in ABM in the presence of VL-type Oag chains that confer resistance to serum...
- Optimization of virulence functions through glucosylation of Shigella LPSNicholas P West
Centre for Molecular Microbiology and Infection, Department of Infectious Diseases, Faculty of Medicine, Flowers Building, Imperial College London, London SW7 2AZ, UK
Science 307:1313-7. 2005..Thus, LPS glucosylation promotes bacterial invasion and evasion of innate immunity, which may have contributed to the emergence of serotype diversity in Shigella...
- Type IV O antigen modification genes in the genome of Shigella flexneri NCTC 8296M M Adams
Division of Biochemistry and Molecular Biology, School of Life Sciences, Faculty of Science, The Australian National University, Canberra, ACT 0200, Australia
Microbiology 147:851-60. 2001..Immunogold labelling and phage sensitivity indicated the presence of both serotype Y and serotype 4a O antigens on the cell surface of the resulting recombinant SFL124 strains, suggesting that only partial serotype ..
- Defective O-antigen polymerization in tolA and pal mutants of Escherichia coli in response to extracytoplasmic stressEnrique D Vines
Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada
J Bacteriol 187:3359-68. 2005..enterica O:54 LPS. Therefore, TolA and Pal are required for the correct surface expression of O antigens that are assembled in a wzy (polymerase)-dependent manner (like those of E...
- O-antigen and virulence profiling of shiga toxin-producing Escherichia coli by a rapid and cost-effective DNA microarray colorimetric methodBeatriz Quiñones
Produce Safety and Microbiology Research Unit, Western Regional Research Center, U S Department of Agriculture Agricultural Research Service Albany, CA, USA
Front Cell Infect Microbiol 2:61. 2012....
- Mutagenesis and chemical cross-linking suggest that Wzz dimer stability and oligomerization affect lipopolysaccharide O-antigen modal chain length controlMagdalene Papadopoulos
Discipline of Microbiology and Immunology, School of Molecular and Biomedical Science, University of Adelaide, Adelaide, South Australia, Australia 5005
J Bacteriol 192:3385-93. 2010..These data suggest that the ability to produce stable dimers may be important in determining Oag modal chain length...
- Dual conserved periplasmic loops possess essential charge characteristics that support a catch-and-release mechanism of O-antigen polymerization by Wzy in Pseudomonas aeruginosa PAO1Salim T Islam
Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada
J Biol Chem 286:20600-5. 2011..These observations support the proposed role of each PL in a catch-and-release mechanism for Wzy-mediated O-antigen polymerization...
- Extensive variation in the O-antigen gene cluster within one Salmonella enterica serogroup reveals an unexpected complex historyLei Wang
Department of Microbiology, The University of Sydney, Sydney, New South Wales 2006, Australia
J Bacteriol 184:1669-77. 2002..Two of the forms lacking a functional wzy(alpha(1-6)) gene have the wzy(alpha(1-2)) gene first described for strain LT2 as rfc, whereas for the third the wzy gene has not been located...
- Detection by multiplex real-time polymerase chain reaction assays and isolation of Shiga toxin-producing Escherichia coli serogroups O26, O45, O103, O111, O121, and O145 in ground beefPina M Fratamico
Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA
Foodborne Pathog Dis 8:601-7. 2011..This work provides a method for detection and isolation in ground beef and potentially other foods of non-O157 STEC of major public health concern...
- The genetic and molecular basis of O-antigenic diversity in Burkholderia pseudomallei lipopolysaccharideApichai Tuanyok
Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona, USA
PLoS Negl Trop Dis 6:e1453. 2012..We postulate that the diversity of LPS may correlate with differential immunopathogenicity and virulence among B. pseudomallei strains...
- Micromethods for the characterization of lipid A-core and O-antigen lipopolysaccharideCristina L Marolda
Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada
Methods Mol Biol 347:237-52. 2006..This chapter describes a collection of methods routinely used in our laboratory for the characterization of LPS in Escherichia coli and other bacteria...
- Relationships of the Escherichia coli O157, O111, and O55 O-antigen gene clusters with those of Salmonella enterica and Citrobacter freundii, which express identical O antigensGabrielle Samuel
School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
J Bacteriol 186:6536-43. 2004..For both the S. enterica O30-C. freundii F90-E. coli O157 group and the S. enterica O50-E. coli O55 group of O antigens, the gene clusters have identical or nearly identical organizations...
- Pseudomonas aeruginosa O-antigen chain length is determined before ligation to lipid A coreCraig Daniels
Department of Microbiology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Environ Microbiol 4:883-97. 2002..We have shown an association between the Wzz proteins and O-antigen polymer chains using immunoprecipitation with anti-O5 O-antigen monoclonal antibody MF15-4. Both Wzz1 and Wzz2 could be co-precipitated with O5 polymer...
- Porphyromonas gingivalis galE is involved in lipopolysaccharide O-antigen synthesis and biofilm formationRyoma Nakao
Department of Bacteriology, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku ku, Tokyo, Japan 162 8640
Infect Immun 74:6145-53. 2006..gingivalis galE gene and virulence phenotypes such as biofilm formation may provide clues for understanding the mechanism of pathogenicity in periodontal disease...
- Micro-array for the identification of Shiga toxin-producing Escherichia coli (STEC) seropathotypes associated with Hemorrhagic Colitis and Hemolytic Uremic Syndrome in humansMarie Bugarel
Afssa French Food Safety Agency, Laboratory for Study and Research on Food Quality and Processes LERQAP, 23 Av du Général De Gaulle, Fr 94706 Maisons Alfort, France
Int J Food Microbiol 142:318-29. 2010..coli (EHEC) serotypes. The genes selected for determination of the O antigens (rfbE(O157), wzx(O26), wzx(O103), wbd1(O111), ihp1(O145), wzx(O121), wzy(O113), wzy(O91), wzx(O104), wzy(O118), ..
- Molecular, genetic, and topological characterization of O-antigen chain length regulation in Shigella flexneriR Morona
Department of Microbiology and Immunology, University of Adelaide, Australia
J Bacteriol 177:1059-68. 1995..flexneri by insertion of a kanamycin resistance cartridge. The resulting strains produced LPS with O antigens of nonmodal chain length, thereby confirming the function of the rol gene product...
- Serotype-converting bacteriophages and O-antigen modification in Shigella flexneriG E Allison
Divn of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, Canberra, ACT, 0200, Australia
Trends Microbiol 8:17-23. 2000..Several serotype-converting phages have been isolated and preliminary characterization has identified the genes involved in O-antigen modification, and has also provided insight into the molecular biology of these phages...
- Identification of a bile-induced exopolysaccharide required for Salmonella biofilm formation on gallstone surfacesRobert W Crawford
Center for Microbial Interface Biology and Department of Molecular Virology, Immunology, and Medical Genetics, The Ohio State University, Columbus, Ohio 43210, USA
Infect Immun 76:5341-9. 2008..These studies provide new therapeutic targets for preventing asymptomatic serovar Typhi gallbladder carriage...
- Phase variation controls expression of Salmonella lipopolysaccharide modification genes by a DNA methylation-dependent mechanismS E Broadbent
Centre for Immunology and Infection, Hull York Medical School and the Department of Biology, University of York, York, UK
Mol Microbiol 77:337-53. 2010..Most Salmonella gtr operons share the key regulatory elements that are identified here as essential for this epigenetic phase variation...
- Effect of the O-antigen length of lipopolysaccharide on the functions of Type III secretion systems in Salmonella entericaStefanie U Hölzer
Mikrobiologisches Institut, Universitatsklinikum Erlangen, Erlangen, Germany
Infect Immun 77:5458-70. 2009..These observations indicate that the architecture of the outer membrane of Salmonella is balanced to permit sufficient T3SS function but also to confer optimal protection against antimicrobial defense mechanisms...
- Burkholderia thailandensis oacA mutants facilitate the expression of Burkholderia mallei-like O polysaccharidesPaul J Brett
Department of Microbiology and Immunology, University of South Alabama, 5851 USA Drive North, Mobile, AL 36688, USA
Infect Immun 79:961-9. 2011..thailandensis OPS antigens and that ZT0715 may provide a safe and cost-effective source of B. mallei-like OPS to facilitate the synthesis of glanders subunit vaccine candidates...
- Identification of a second lipopolysaccharide in Porphyromonas gingivalis W50Minnie Rangarajan
Centre for Infectious Disease, Institute of Cell and Molecular Science, Barts and The London Queen Mary s School of Medicine and Dentistry, 4 Newark Street, London E1 2AT, United Kingdom
J Bacteriol 190:2920-32. 2008..gingivalis has dual specificity for both O-antigen and APS repeating units...
- Bacterial glycans: key mediators of diverse host immune responsesLaurie E Comstock
Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
Cell 126:847-50. 2006..Here, we discuss the diverse interactions between bacterial glycans and the host immune system...
- WaaL of Pseudomonas aeruginosa utilizes ATP in in vitro ligation of O antigen onto lipid A-corePriyanka D Abeyrathne
Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada
Mol Microbiol 65:1345-59. 2007..A waaL mutant of P. aeruginosa could not be cross-complemented by waaL of Escherichia coli, which suggested that each of these proteins has specificity for its cognate core oligosaccharide...
- The PmrA/PmrB and RcsC/YojN/RcsB systems control expression of the Salmonella O-antigen chain length determinantMonica A Delgado
Department of Molecular Microbiology, Washington University School of Medicine, 660 S Euclid, St Louis, MO 63110, USA
Mol Microbiol 60:39-50. 2006..That multiple regulatory systems control wzzst expression suggests that the Wzzst protein is required under different environmental conditions...
- Export of O-specific lipopolysaccharideMiguel A Valvano
Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1 Canada
Front Biosci 8:s452-71. 2003..side of the plasma membrane as well as several different translocation pathways that deliver the nascent O antigens in a glycolipid form to the periplasmic side of the plasma membrane...
- A novel glucosyltransferase involved in O-antigen modification of Shigella flexneri serotype 1cRobert M Stagg
Biochemistry and Molecular Biology, Research School of Biology, The Australian National University, Canberra, ACT 0200, Australia
J Bacteriol 191:6612-7. 2009..flexneri from outside the species. Analysis of surrounding sequence suggests that the gtrIC cluster arrived via a novel bacteriophage that was subsequently rendered nonfunctional by a series of insertion events...
- Structural and genetic analyses of O polysaccharide from Actinobacillus actinomycetemcomitans serotype fJ B Kaplan
Department of Oral Pathology, Biology and Diagnostic Sciences, New Jersey Dental School, Newark, New Jersey 07103 2714, USA
Infect Immun 69:5375-84. 2001..The finding of an A. actinomycetemcomitans serotype showing serological cross-reactivity with anti-serotype b-specific antiserum suggests that a reevaluation of strains previously classified as serotype b may be warranted...
- Organization of Escherichia coli O157 O antigen gene cluster and identification of its specific genesL Wang
Department of Microbiology, The University of Sydney, Sydney, New South Wales 2006, Australia
Infect Immun 66:3545-51. 1998..coli or, once flanking genes are identified, clones of any gram-negative bacterium...
- Structure of the O-polysaccharide of Aeromonas hydrophila O:34; a case of random O-acetylation of 6-deoxy-L-taloseYuriy A Knirel
N D Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, Moscow 119991, Russia
Carbohydr Res 337:1381-6. 2002....
- Mice vaccinated with the O-antigen of Francisella tularensis LVS lipopolysaccharide conjugated to bovine serum albumin develop varying degrees of protective immunity against systemic or aerosol challenge with virulent type A and type B strains of the pathJ Wayne Conlan
National Research Council Canada, Institute for Biological Sciences, Ont, K1A OR6, Ottawa, Canada
Vaccine 20:3465-71. 2002..The results suggest that the O-antigen of F. tularensis could be considered as a potential component of a subunit vaccine against type B, but not type A strains of F. tularensis...
- Polysaccharides and virulence of Burkholderia pseudomalleiM Sarkar-Tyson
Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, UK
J Med Microbiol 56:1005-10. 2007..However, immunization with killed polysaccharide mutant strains conferred different degrees of protection, demonstrating the immunological importance of the polysaccharide clusters on the surface of B. pseudomallei...
- The O antigen is a critical antigen for the development of a protective immune response to Bordetella parapertussisXuqing Zhang
Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, 115 Henning Building, University Park, PA 16802, USA
Infect Immun 77:5050-8. 2009..parapertussis. These data suggest that the O antigen is a critical protective antigen of B. parapertussis and its inclusion can substantially improve whooping cough vaccine efficacy against this pathogen...
- 'Form variation' of the O12 antigen is critical for persistence of Salmonella Typhimurium in the murine intestineLydia M Bogomolnaya
Department of Microbial and Molecular Pathogenesis, College of Medicine, Texas A and M University System Health Science Center, College Station, TX 77843 1114, USA
Mol Microbiol 70:1105-19. 2008..Our data suggest that the 12-2 antigen is a S. enterica subspecies I-specific LPS modification that enhances long-term intestinal colonization, and is in contrast to the role of O-antigen variation described for Shigella...
- Genetic modification of the O-polysaccharide of Francisella tularensis results in an avirulent live attenuated vaccineTae Hyun Kim
Department of Microbiology and Molecular Genetics, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02215, USA
J Infect Dis 205:1056-65. 2012..Francisella tularensis, the causative agent of tularemia, is a highly virulent microbe. One significant virulence factor of F. tularensis is the O-polysaccharide (O-PS) portion of the organism's lipopolysaccharide...
- O-polysaccharide glycosylation is required for stability and function of the collagen adhesin EmaA of Aggregatibacter actinomycetemcomitansGaoyan Tang
Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, Vermont, USA
Infect Immun 80:2868-77. 2012..The glycan modification of EmaA appears to be required for collagen binding activity and protection of the protein against degradation by proteolytic enzymes...
- The Wzx translocases for Salmonella enterica O-antigen processing have unexpected serotype specificityYaoqin Hong
School of Molecular Bioscience, The University of Sydney, Sydney, NSW, Australia
Mol Microbiol 84:620-30. 2012..widely thought to be specific only for the first sugar of the repeat unit, despite extensive variation in both O antigens and Wzx translocases. However, we found for S...
- Polysaccharide co-polymerases: the enigmatic conductors of the O-antigen assembly orchestraSergei Kalynych
Department of Biochemistry, McGill University, Montreal, Quebec H3G0B1, Canada
Protein Eng Des Sel 25:797-802. 2012..Here we review the current knowledge of structure and function of these polysaccharide length regulators...
- Structural analysis of the O-antigen polysaccharide from Escherichia coli O152Ulrika Olsson
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, S 106 91 Stockholm, Sweden
Carbohydr Res 340:167-71. 2005..coli O173. The cross-reactivity between E. coli O152 and E. coli O3 may be explained by structural similarities in the branching region of their O-antigen polysaccharides...
- Comparison of O-antigen gene clusters of Escherichia coli (Shigella) sonnei and Plesiomonas shigelloides O17: sonnei gained its current plasmid-borne O-antigen genes from P. shigelloides in a recent eventJ G Shepherd
Department of Microbiology, The University of Sydney, Sydney, New South Wales 2006, Australia
Infect Immun 68:6056-61. 2000..By sequencing the chromosomal O-antigen gene cluster of P. shigelloides O17 and comparing it with that of Sonnei, we showed that Sonnei gained its O-antigen genes recently...
- C-terminal half of Salmonella enterica WbaP (RfbP) is the galactosyl-1-phosphate transferase domain catalyzing the first step of O-antigen synthesisL Wang
Department of Microbiology, The University of Sydney, New South Wales, Australia
J Bacteriol 178:2598-604. 1996..We now propose that the T block operates prior to the flippase function, probably at the release of undecaprenyl pyrophosphate-linked galactose from WbaP...
- A rapid procedure for the detection and isolation of enterohaemorrhagic Escherichia coli (EHEC) serogroup O26, O103, O111, O118, O121, O145 and O157 strains and the aggregative EHEC O104:H4 strain from ready-to-eat vegetablesMarkus Tzschoppe
National Reference Laboratory for Escherichia coli, Unit 41 Microbial Toxins, Germany
Int J Food Microbiol 152:19-30. 2012..Aggregative EHEC O104:H4 could be detected and isolated from a sample of sprouted seeds which was suspected as vector of transmission of EHEC O104 to humans...
- Salmonella enterica serovar enteritidis core O polysaccharide conjugated to H:g,m flagellin as a candidate vaccine for protection against invasive infection with S. enteritidisRaphael Simon
Center for Vaccine Development, University of Maryland, Baltimore, Baltimore, MD 21201, USA
Infect Immun 79:4240-9. 2011..Mice immunized with flagellin alone, COPS-CRM₁₉₇, or COPS-flagellin conjugates were significantly protected from lethal challenge with wild-type S. Enteritidis (80 to 100% vaccine efficacy)...
- Lipopolysaccharide O-antigen promotes persistent murine bacteremiaJoanelle Z Lugo
Department of Emergency Medicine, University of Michigan, Ann Arbor, MI 48109, USA
Shock 27:186-91. 2007....
- Structural and genetic characterization of Escherichia coli O99 antigenAndrei V Perepelov
N D Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
FEMS Immunol Med Microbiol 57:80-7. 2009..Our findings indicate that in E. coli O99, the synthesis and translocation of the O-antigen occurs by an ABC transporter-dependent process...
- Sequence of the Escherichia coli O26 O antigen gene cluster and identification of O26 specific genesJocelyne M D'Souza
Department of Microbiology, School of Molecular and Microbial Biosciences G08, University of Sydney, Sydney, NSW 2006, Australia
Gene 297:123-7. 2002Escherichia coli associated with outbreaks of gastroenteritis and hemolytic uremic syndrome include clones with O antigens O157 and O111...
- O antigen protects Bordetella parapertussis from complementElizabeth M Goebel
Department of Veterinary and Biomedical Sciences, Pennsylvania State University, 115 Henning Building, University Park, PA 16802, USA
Infect Immun 76:1774-80. 2008..These data indicate that O antigen enables B. parapertussis to efficiently colonize the lower respiratory tract by protecting against complement-mediated control and clearance...
- O antigen gene clusters of Yersinia pseudotuberculosisPeter R Reeves
School of Molecular and Microbial Biosciences, University of Sydney, NSW2006, Australia
Adv Exp Med Biol 529:199-206. 2003
- Sequence and analysis of the O antigen gene (rfb) cluster of Escherichia coli O111D A Bastin
Department of Microbiology, University of Sydney, New South Wales, Australia
Gene 164:17-23. 1995The O antigens found in Salmonella enterica (Se) and Escherichia coli (Ec) show a great deal of diversity, and only three structures are known to be common to both genera...
- Nonreducing terminal modifications determine the chain length of polymannose O antigens of Escherichia coli and couple chain termination to polymer export via an ATP-binding cassette transporterBradley R Clarke
Department of Microbiology, University of Guelph, Guelph, Ontario N1G 2W1, Canada
J Biol Chem 279:35709-18. 2004The chain length of bacterial lipopolysaccharide O antigens is regulated to give a modal distribution that is critical for pathogenesis...
- Flavonoid-inducible modifications to rhamnan O antigens are necessary for Rhizobium sp. strain NGR234-legume symbiosesW J Broughton
LBMPS, Universite de Geneve, Switzerland
J Bacteriol 188:3654-63. 2006..Mutation of either pathway reduces rhamnan production. Coordination of rhamnan synthesis with rhizobial release from infection threads is thus part of the symbiotic interaction...
- Identification, characterization and immunogenicity of an O-antigen capsular polysaccharide of Francisella tularensisMichael A Apicella
Department of Microbiology, University of Iowa, Iowa City, Iowa, United States of America
PLoS ONE 5:e11060. 2010..tularensis LVS. Active immunization of BALB/c mice with 10 microg of capsule showed a similar level of protection. These studies demonstrate that F. tularensis produces an O-antigen capsule that may be the basis of a future vaccine...
- Post-assembly modification of Bordetella bronchiseptica O polysaccharide by a novel periplasmic enzyme encoded by wbmEJerry D King
Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, United Kingdom
J Biol Chem 284:1474-83. 2009....
- Comparative genomics of the classical Bordetella subspecies: the evolution and exchange of virulence-associated diversity amongst closely related pathogensJihye Park
Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, USA
BMC Genomics 13:545. 2012..The compelling picture from previous comparisons of the three sequenced genomes was of genome degradation, with substantial loss of genome content (up to 24%) associated with adaptation to humans...
- Phase 1 and phase 2 studies of Salmonella enterica serovar paratyphi A O-specific polysaccharide-tetanus toxoid conjugates in adults, teenagers, and 2- to 4-year-old children in VietnamE Y Konadu
National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2720, USA
Infect Immun 68:1529-34. 2000..0001); a second injection did not elicit a booster response. Representative sera from all groups had bactericidal activity that could be adsorbed by S. enterica serovar Paratyphi A LPS...
- Lipopolysaccharide heterogeneity in Brucella strains isolated from marine mammalsSylvie Baucheron
Institut National de la Recherche Agronomique, Unité de Pathologie Aviaire et Parasitologie, Nouzilly, France
Res Microbiol 153:277-80. 2002..Thus, S-LPSs of the marine mammal isolates show heterogeneity with regard to their O-PS C epitope content and their average size...
- Novel role of the lipopolysaccharide O1 side chain in ferric siderophore transport and virulence of Vibrio anguillarumTimothy J Welch
Department of Molecular Microbiology and Immunology, Oregon Health and Science University 3181 Sam Jackson Park Road, Portland, OR 97239, USA
Infect Immun 73:5864-72. 2005..anguillarum: serum resistance and anguibactin-mediated iron transport. These factors allow V. anguillarum to survive in serum and multiply in the iron-limiting milieu of the host vertebrate...
- Genomic comparison of the O-antigen biosynthesis gene clusters of Escherichia coli O55 strains belonging to three distinct lineagesAtsushi Iguchi
Division of Bioenvironmental Science, Frontier Science Research Center, University of Miyazaki, Miyazaki, Japan
Microbiology 154:559-70. 2008..coli lineages. The data provide new insights into the mechanisms involved in the generation of a wide variety of O-serotypes in Gram-negative bacteria...
- Putative O-antigen transport genes within the rfb region of Vibrio cholerae O1 are homologous to those for capsule transportP A Manning
Department of Microbiology and Immunology, University of Adelaide, Australia
Gene 158:1-7. 1995..The function of RfbG is unknown, but it would appear to be a relatively hydrophilic protein and we can only speculate that it may be either a specific transferase or possibly the O-antigen polymerase...
- Evidence for horizontal gene transfer of two antigenically distinct O antigens in Bordetella bronchisepticaAnne M Buboltz
Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, 16802, USA
Infect Immun 77:3249-57. 2009..from strain RB50 (O1), 1289 (O2), or RB50Deltawbm (O antigen deficient), our data indicate that these O antigens do not confer cross-protection in vivo...
- O-antigen diversity and lateral transfer of the wbe region among Vibrio splendidus isolatesHans Wildschutte
Civil and Environmental Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
Environ Microbiol 12:2977-87. 2010..Our results demonstrate O-antigen hyper-variability among these environmental strains and suggest that frequent lateral gene transfer generates wbe extensive diversity among V. splendidus and its close relatives...
- A new ethanolamine phosphate-containing variant of the O-antigen of Shigella flexneri type 4aAndrei V Perepelov
N D Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation
Carbohydr Res 344:1588-91. 2009..Phosphorylation has not been hitherto reported in any S. flexneri O-antigen. Reinvestigation of the O-specific polysaccharide of S. flexneri type 4b showed that it is not phosphorylated and confirmed its structure established earlier...
- Membrane topology of the Salmonella enterica serovar Typhimurium Group B O-antigen translocase WzxMonica M Cunneen
School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW, Australia
FEMS Microbiol Lett 287:76-84. 2008..Enzyme activity of these, and full-length C-terminal fusion proteins, confirmed the 12-TMS topology for this Wzx, and also indicated that the C-terminus was located within the cytoplasm, which is consistent with the predicted topology...
- Molecular evolution of the GDP-mannose pathway genes (manB and manC) in Salmonella entericaS O Jensen
Department of Microbiology (G08, University of Sydney, New South Wales 2006, Australia
Microbiology 147:599-610. 2001..pathway in Salmonella enterica was studied via sequencing manB and manC genes from 13 representative strains for O antigens containing mannose and/or sugar derivatives of GDP-D-mannose...
- O-antigen-negative Salmonella enterica serovar Typhimurium is attenuated in intestinal colonization but elicits colitis in streptomycin-treated miceKarin Ilg
Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, ETH Zurich, Zurich, Switzerland
Infect Immun 77:2568-75. 2009..Our experiments prove that the presence of complete LPS is not essential for in vitro invasion or for triggering acute colitis...
- The Klebsiella pneumoniae O2a antigen defines a second mechanism for O antigen ATP-binding cassette transportersVeronica Kos
Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada
J Biol Chem 284:2947-56. 2009..ATP-binding cassette (ABC) transporter-dependent mechanism is responsible for the biosynthesis of polysaccharide O antigens in the lipopolysaccharides of many Gram-negative bacteria...
- The role of lipopolysaccharide moieties in macrophage response to Escherichia coliKatalin Eder
Biological Research Center of Hungarian Academy of Sciences, Institute of Biochemistry, Temesvari krt 62, Szeged H 6726, Hungary
Biochem Biophys Res Commun 389:46-51. 2009..The most ancient part, lipid A is crucial in evoking immediate TNF release and activation of NF-kappaB. The O-antigen inhibits phagocytosis, leading to immune evasion...
- The Klebsiella pneumoniae O antigen contributes to bacteremia and lethality during murine pneumoniaSunita Shankar-Sinha
Department of Emergency Medicine, School of Medicine, University of Michigan, Ann Arbor, Michigan, USA
Infect Immun 72:1423-30. 2004..These results suggest that during murine pneumonia caused by K. pneumoniae, O antigen contributes to lethality by increasing the propensity for bacteremia and not by significantly changing the early course of intrapulmonary infection...
- Structure-guided investigation of lipopolysaccharide O-antigen chain length regulators reveals regions critical for modal length controlSergei Kalynych
Department of Biochemistry, McGill University, Montreal, Quebec, Canada
J Bacteriol 193:3710-21. 2011..Together, our data suggest that chain length determination depends on regions that likely contribute to stabilize a supramolecular complex...
- Vibrio anguillarum resistance to rainbow trout (Oncorhynchus mykiss) serum: role of O-antigen structure of lipopolysaccharideH T Boesen
Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Frederiksberg C, Denmark
Infect Immun 67:294-301. 1999..Strains grown in glucose-enriched medium became sensitive to rainbow trout serum killing, indicating that the high-molecular-weight O-antigen side chains prevented the activated complement from damaging the bacterium...
- Characterization of functional oligosaccharide mimics of the Shigella flexneri serotype 2a O-antigen: implications for the development of a chemically defined glycoconjugate vaccineArmelle Phalipon
Unite de Pathogenie Microbienne Moleculaire, Institut National de la Santé et de la Recherche Médicale Unité 389
J Immunol 176:1686-94. 2006..A pentadecasaccharide representing three biological repeating units was identified as a potential candidate for further development of a chemically defined glycoconjugate vaccine against S. flexneri 2a infection...
- The wbiA locus is required for the 2-O-acetylation of lipopolysaccharides expressed by Burkholderia pseudomallei and Burkholderia thailandensisPaul J Brett
Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, 3330 Hospital Drive NW, T2N 4N1, Calgary, AB, Canada
FEMS Microbiol Lett 218:323-8. 2003....
- Protection of mice against Salmonella typhimurium with an O-specific polysaccharide-protein conjugate vaccineD C Watson
Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, Bethesda, Maryland 20892
Infect Immun 60:4679-86. 1992..These results indicate that an O-SP-TT conjugate, when given by a route and formulation acceptable for human use, protects mice against challenge with S. typhimurium...
- Inducible serum resistance in Salmonella typhimurium is dependent on wzz(fepE)-regulated very long O antigen chainsGerald L Murray
Australian Bacterial Pathogenesis Program, Discipline of Microbiology and Immunology, School of Molecular and Biomedical Science, The University of Adelaide, Adelaide 5005, Australia
Microbes Infect 7:1296-304. 2005..Since systemic infection occurs in a small but significant percentage of human non-typhoid salmonellosis, the phenomenon identified in this study may be significant during the bacteraemic phase of infection...
- Serotype 1a O-antigen modification: molecular characterization of the genes involved and their novel organization in the Shigella flexneri chromosomeP Adhikari
Division of Biochemistry and Molecular Biology, Faculty of Science, The Australian National University, Canberra, ACT 0200, Australia
J Bacteriol 181:4711-8. 1999..6-kb region of the Y53 chromosome is unique and suggests that the serotype conversion genes were originally brought into the host by a bacteriophage. Several features of this region are also characteristic of pathogenicity islands...
- Membrane topology and identification of critical amino acid residues in the Wzx O-antigen translocase from Escherichia coli O157:H4Cristina L Marolda
Center for Human Immunology, Siebens Drake Research Institute, Departments of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada
J Bacteriol 192:6160-71. 2010..We propose that the functional requirement of charged residues at both sides of the membrane and in two TM helices could be important to allow the passage of the Und-PP-linked saccharide substrate across the membrane...
- Growth-phase regulation of lipopolysaccharide O-antigen chain length influences serum resistance in serovars of SalmonellaDenisse Bravo
Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Quimicas y Farmaceuticas, Universidad de Chile, PO Box 174 Correo 22, Santiago, Chile
J Med Microbiol 57:938-46. 2008..In contrast to the relevant role for high-molecular-mass OAg molecules, the presence of Vi antigen did not contribute to serum resistance of clinical isolates of serovar Typhi...
- The biosynthesis and biological role of lipopolysaccharide O-antigens of pathogenic YersiniaeMikael Skurnik
Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki and Helsinki University Central Hospital Laboratory Diagnostics, P O Box 63, FIN 00014 Helsinki, Finland
Carbohydr Res 338:2521-9. 2003....
- New rapid test for paratyphoid a fever: usefulness, cross-detection, and solutionFrankie C H Tam
Clinical Immunology Unit, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong
Diagn Microbiol Infect Dis 62:142-50. 2008....
- Lipopolysaccharide O antigen status of Yersinia enterocolitica O:8 is essential for virulence and absence of O antigen affects the expression of other Yersinia virulence factorsJosé Antonio Bengoechea
Unidad de Investigación and Institut Universitari d Investigacions en Ciències de la Salut, Hospital Son Dureta, Palma de Mallorca, Spain
Mol Microbiol 52:451-69. 2004..Together these findings suggest that the absence of O antigen in the outer membrane of Yersinia either directly or indirectly, for example through a cellular or membrane stress, could act as a regulatory signal...
- Presence or absence of lipopolysaccharide O antigens affects type III secretion by Pseudomonas aeruginosaD K Augustin
Biology Department, California State University, San Francisco, CA 94142, USA
J Bacteriol 189:2203-9. 2007..These results suggest the existence of a cooperative association between LPS O-antigen structure and the TTSS in both laboratory and clinical isolates of P. aeruginosa...
- Repeat unit polysaccharides of bacteria: a model for polymerization resembling that of ribosomes and fatty acid synthetase, with a novel mechanism for determining chain lengthD A Bastin
Department of Microbiology, University of Sydney, NSW, Australia
Mol Microbiol 7:725-34. 1993..The CLD is not O-antigen or species-specific but the modal value does depend on the source of the cld gene...
- Characterization of the O-antigen polymerase (Wzy) of Francisella tularensisTae Hyun Kim
Department of Microbiology and Molecular Genetics, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 285:27839-49. 2010..Topology models indicate that these amino acids most likely lie in close proximity on the bacterial surface...
- Yersinia enterocolitica serum resistance proteins YadA and ail bind the complement regulator C4b-binding proteinVesa Kirjavainen
Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki, Finland
PLoS Pathog 4:e1000140. 2008..These findings show that Y. enterocolitica uses two proteins, YadA and Ail, to bind C4bp. Binding of C4bp could help Y. enterocolitica to evade complement-mediated clearance in the human host...
- The role of cellulose and O-antigen capsule in the colonization of plants by Salmonella entericaJeri D Barak
Produce Safety and Microbiology Research Unit, United States Department of Agriculture Agricultural Research Service, Albany, CA 94710, USA
Mol Plant Microbe Interact 20:1083-91. 2007..Outside of animal hosts, S. enterica appears to rely on an arsenal of adhesins to persist on plants, which can act as vectors and perpetuate public health concerns...
- The activity of a putative polyisoprenol-linked sugar translocase (Wzx) involved in Escherichia coli O antigen assembly is independent of the chemical structure of the O repeatM F Feldman
Instituto de Investigaciones Bioquimicas Fundacion Campomar, Buenos Aires, Argentina
J Biol Chem 274:35129-38. 1999....
- Development of PCR assays targeting genes in O-antigen gene clusters for detection and identification of Escherichia coli O45 and O55 serogroupsChitrita Debroy
Gastroenteric Disease Center, The Pennsylvania State University, Wiley Lab, Wiley Lane, University Park, PA 16802, USA
Appl Environ Microbiol 71:4919-24. 2005..coli O55 wzx and wzy genes were used to detect the organisms in fecal samples spiked at levels of 10(6) and 10(8) CFU/0.2 g feces. Thus, the PCR assays can be used to detect and identify E. coli serogroups O45 and O55...
- Evasion of complement-mediated lysis and complement C3 deposition are regulated by Francisella tularensis lipopolysaccharide O antigenCorey D Clay
Department of Internal Medicine, Ohio State University, Columbus, OH 43210, USA
J Immunol 181:5568-78. 2008....
- Mechanisms of adaptive immunity to P. aeruginosa in the lungGregory P Priebe; Fiscal Year: 2013..Finally, we will assess whether expression of heterologous O antigens by the vaccine or changes in its LPS lipid A acylation pattern can enhance its effectiveness...
- X-ray Studies of Sugar-Modifying EnzymesHazel Holden; Fiscal Year: 2013....
- Phospholipid flip-flop in biogenic membranesAnant K Menon; Fiscal Year: 2013..coli lipopolysaccharide. We are interested in identifying the transport proteins that catalyze lipid flipping in yeast and mammals and understanding how they work. ..
- LIPID A MODIFICATION SYSTEMS IN GRAM-NEGATIVE BACTERIAPei Zhou; Fiscal Year: 2012....
- GENETICS AND VIRULENCE OF PNEUMOCOCCAL CAPSULAR TYPESJANET L YOTHER; Fiscal Year: 2012..By understanding the mechanisms involved in this process, we may be better able to develop means for treating and preventing these infections. ..
- Research and Development of a Novel System to Produce Polysaccharide Conjugate VaPeng George Wang; Fiscal Year: 2013....
- BIOSYNTHESIS AND FUNCTION OF LIPOPOLYSACCHARIDESPei Zhou; Fiscal Year: 2013....
- A Novel Surface Sensing Pathway in Proteus mirabilisPhilip N Rather; Fiscal Year: 2013..To address this gap, RNA-Seq will be used to identify genes that are specifically regulated by the response regulator RcsB. ..
- Live Attenuated Recombinant Bacterial Delivery of Polysaccharide Vaccine AntigensJoanna B Goldberg; Fiscal Year: 2010..conjugate vaccines including subtype specificity and subtype inhibition, and the exclusion of acid-liable O antigens. The specific aims of this grant are to (1) determine the basis for protective immunity to P...
- Mass Spectrometric Characterization of LipopolysaccharidesJennifer S Brodbelt; Fiscal Year: 2013..Specific biological problems include correlation of the structura changes of lipid A with the inflammatory response, unraveling the biosynthetic pathway of LPS, and elucidating the mechanism of resistance to antimicrobial peptides. ..
- Crystallization of outer membrane proteins for export of polysaccharide capsuleMark A Saper; Fiscal Year: 2012....
- O-Specific Polysaccharide Responses and CholeraEdward T Ryan; Fiscal Year: 2013..abstract_text> ..
- Bacterial Surface Structures of Helicobacter Pylori and Campylobacter JejuniMICHAEL STEPHEN TRENT; Fiscal Year: 2013..Bacteria modify their LPS structure which directly impacts disease. This proposal will help determine how bacteria associated with human disease modify their LPS structure possibly leading to novel therapies. ..
- The Outer Surface of Vibrio choleraeMICHAEL STEPHEN TRENT; Fiscal Year: 2012..cholerae lipid A species;(ii) environmental regulation of V. cholerae lipid A structure;(iii) enzymatic modification of V. cholerae lipid A;and (iv) Toll-like receptor mediated immune activation by V. cholerae LPS. ..
- Remodeling Salmonella outer membrane for vaccine developmentQingke Kong; Fiscal Year: 2013..The success of this project could lead to efficacious RASV with broad spectrum protection against enteric bacteria for human use. ..
- Development of O Antigen-based Vaccines Against Q FeverGuoquan Zhang; Fiscal Year: 2013..burnetii infection, which will provide novel evidence to prove the concept that C. burnetii O antigen-based vaccines can confer protective immunity against Q fever. ..
- Bacteriolytic phage enzymes as novel antibacterials against Yersinia pestisIan Molineux; Fiscal Year: 2009..pestis infections. These in vivo studies will parallel those involving lysins using the mouse model of plague infection. ..
- Genome Evolution in Pathogens: The roles of predator-prey interactionsJeffrey G Lawrence; Fiscal Year: 2010....
- Genetics and biosynthesis of an O antigen essential for symbiosisKENNETH D NOEL; Fiscal Year: 2010..It also is important in other interactions with animal and plant hosts. This property is a type of carbohydrate chain on the surfaces of many bacteria. The question addressed in this work is how this chain is synthesized. ..
- Production of Polysaccharide-Protein Conjugates as VaccinesWen Yi; Fiscal Year: 2009..In this program, we develop a novel system to produce conjugate vaccines in a facile and widely applicable manner compared to the traditional chemical approach. ..
- POPULATION BIOLOGY OF SURFACE POLYSACCHARIDES IN CHOLERAJohn Morris; Fiscal Year: 2004..cholerae surface polysaccharides and, in turn, for the emergence of V. cholerae strains which can form the basis for new cholera pandemics. ..
- ACID INDUCIBLE RESPONSES IN H PYLORICatherine McGowan; Fiscal Year: 2003..The proposed work will further elucidate the mechanisms used by H. pylori to colonize and persist within the gastric environment and will lead to improved understanding of how H. pylori causes disease. ..
- COMPUTATIONAL ANALYSIS OF CARBOHYDRATE ANTIGENICITYRobert Woods; Fiscal Year: 2000..A better understanding of the conformational properties of the serotypes may provide a basis for rational vaccine design. ..
- Molecular Pathogenesis of LeptospirosisMichael Lovett; Fiscal Year: 2009..The surface antigenic structure and biological properties of HTL and IVCL will be compared. ..
- Structure, mechanism and evolution of KDO8P synthaseDOMENICO GATTI; Fiscal Year: 2007..abstract_text> ..
- CAMPYLOBACTER PYLORI IN GASTROINTESTINAL DISEASEJOHN PENNER; Fiscal Year: 1990..The longer term objective is to conduct epidemiological studies to trace the sources and routes of transmission of C. pyloridis and obtain convincing evidence to delineate the role of the species in diseases of the stomach and duodenum...
- Ecological Fitness of Vibrios and the Emergence of Pathogenic TraitsHans Wildschutte; Fiscal Year: 2009..Results may lead to insights on how to monitor and predict bacterial outbreaks. ..
- Development of killed vaccine for Vibrio cholerae O139Jan Holmgren; Fiscal Year: 2007..unreadable] [unreadable]..
- Synthesis of P.Aeruginosa LPS and its Role in InfectionJOANNA GOLDBERG; Fiscal Year: 2004..aeruginosa strains that have a defective LPS with few or no O antigens; these infections remain localized to the lung. In other P...
- Pseudomonas aeruginasa LPS: A Post-Genomic AnalysisJOANNA GOLDBERG; Fiscal Year: 2004..abstract_text> ..
- REGULATION OF O-ANTIGEN SYNTHESIS IN SALMONELLAMary Osborn; Fiscal Year: 1993....
- Pseudomonas aeruginosa 1244 Pilin GlycosylationPeter Castric; Fiscal Year: 2007..aeruginosa will be established. The membrane topology of this enzyme will also be determined. Finally, the subcellular location of the P. aeruginosa 1244 pilin glycosylation reaction established. [unreadable] [unreadable]..
- Studies on the glycosylation of P. aeruginosa piliPeter Castric; Fiscal Year: 2002..The project will determine the step in O-antigen biosynthesis that produces the glycan and the subcellular location of pilin glycosylation. ..
- ASSAYS FOR ENDOTOXIN IN PLASMARobert Munford; Fiscal Year: 1980....
- BACTERIAL MODULATION OF LUNG INFLAMMATORY RESPONSEThomas Russo; Fiscal Year: 2004..g. hemolysin) and bacterially induced host response elements in directly mediating the pathogenesis of lung injury. ..
- BRAIN ASSOCIATED HEMATOPOIETIC ANTIGENSEDWARD GOLUB; Fiscal Year: 1980..Bone marrow cells are being cultured in the presence of thymic epithelium cells and the expression of brain associated O antigen is being determined...
- Resistant E.coli in Humans and PoultryJames Johnson; Fiscal Year: 2005..Does the research address a current and compelling problem of antimicrobial resistance that is of high public health importance and for which research is needed? [yes]. ..
- Adaptive immunity of P. aeruginosa in cystic fibrosisGREGORY PRIEBE; Fiscal Year: 2006..Knowledge gained from these studies will elucidate new strategies for the prevention and treatment of P. aeruginosa infections in the setting of CF. [unreadable] [unreadable] [unreadable]..
- Cellular immunity to P. aeruginosa candidate vaccinesGREGORY PRIEBE; Fiscal Year: 2005..As a specialist in pediatric critical care and infectious diseases, his long-term goal is to develop effective immune-based interventions to prevent or ameliorate the consequences of P. aeruginosa infections. ..
- Comparative Immunobiology of the bordetellaeEric Harvill; Fiscal Year: 2007..Similar mechanisms are likely to be involved in respiratory immunity to other organisms, including those considered potential bioterrorism threats. ..
- Biofilm growth and detachment of an oral pathogenJeffrey Kaplan; Fiscal Year: 2007..abstract_text> ..
- LIPID A MODIFICATION SYSTEMS IN GRAM-NEGATIVE BACTERIACHRISTIAN RAETZ; Fiscal Year: 2008..leguminosarum lipid A; and IV) i characterization of enzymes that incorporate the unique inner core sugars of/?, leguminosarum LPS. ..
- EMERGENCE OF HUMAN PATHOGENS IN THE GENUS BORDETELLAEric Harvill; Fiscal Year: 2007..Finally, these data will parameterize models of the within- and between-host dynamics of these reemerging human pathogens. ..
- Center for the Study of Natural Genetic VariationMaynard Olson; Fiscal Year: 2005..abstract_text> ..
- Genetic clustering and virulence of Y. pestis strainsAlexander Sulakvelidze; Fiscal Year: 2005..The generated data will also play a critical role in our designing appropriate future studies, and in expanding our Y. pestis research program at the University of Maryland School of Medicine. ..
- Male Fertility & Protein Expression in SpermatogenesisPAUL RICK; Fiscal Year: 2004..abstract_text> ..
- Comparative Genomics of Lyme Disease SpirochetesSherwood Casjens; Fiscal Year: 2004..burgdorferi population. These findings will impact the study of the molecular pathogenesis of Lyme disease in many ways. ..
- ANTIGEN DELIVERY SYSTEM DESIGN FOR HOST DEFENSE ANALYSISRoy Curtiss; Fiscal Year: 2004..As improved antigen delivery systems are discovered, it is anticipated that they will construct S. typhi or S. paratyphi A derivatives thereof for eventual clinical studies in humans. ..
- Genetics of Survival of Yersinia pseudotuberculosisJoan Mecsas; Fiscal Year: 2002..In addition, studies of yop mutant Yersinia strains in mouse strains with specific immune defects may indicate the facets of the immune system targeted by each Yop. ..
- FOURTEENTH INTERNATIONAL PATHOGENIC NEISSERIA CONFERENCEMichael Apicella; Fiscal Year: 2004..6. To develop new research agenda for the study of the pathogenic Neisseria and to recommend new and future research initiatives and prioritize these. ..
- Inhibitors of Yersinia Yop Type III Secretion(RMI)Joan Mecsas; Fiscal Year: 2005..In addition, these inhibitors will be valuable tools to probe the structure and function of type III secretion systems encoded by Yersinia species and other Gram-negative bacterial pathogens. ..
- Bacteriophage-based probiotic preparations and their effect on the gut microfloraAlexander Sulakvelidze; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Acquired Immunity and Vaccination for P. aeruginosaGerald Pier; Fiscal Year: 2009..aeruginosa pathogenesis, development of acquired immunity and the potential to develop effective reagents for active and passive immunization to augment the prevention and treatment off, aeruginosa infections. ..
- UW Genome Center Large-scale Sequencing ProgramMaynard Olson; Fiscal Year: 2006..Other capabilities of the UWGC such as its automated finishing capacity will be made available to the NHGRI network on an as-needed basis. [unreadable] [unreadable] [unreadable]..
- S. typhimiurium Vaccine Against Bacterial EnteropathogensRoy Curtiss; Fiscal Year: 2007..abstract_text> ..
- Non-replicative Vaccine for Human BrucellosisRamesh Vemulapalli; Fiscal Year: 2011..At present there is no vaccine available for prevention of brucellosis in humans. Research proposed in this application focuses on developing a highly effective and safe Brucella vaccine for human use. ..
- Salmonella anti-influenza DNA & antigen delivery vaccineRoy Curtiss; Fiscal Year: 2008..abstract_text> ..
- DNA packaging and delivery by dsDNA virusesSherwood Casjens; Fiscal Year: 2007..The aims of the project will be pursued through a combination of genetic, biochemical, biophysical and structural analysis strategies. ..
- CFTR AND INFECTION OF CYSTIC FIBROSIS PATIENTSGerald Pier; Fiscal Year: 2009..Overall, an enhanced understanding of the normal resistance to P. aeruginosa infection and the role of CFTR in this process will be obtained from the proposed aims. ..
- Proteomic studies of pathogenic LeptospiraJarlath Nally; Fiscal Year: 2004....