o antigens

Summary

Summary: The lipopolysaccharide-protein somatic antigens, usually from gram-negative bacteria, important in the serological classification of enteric bacilli. The O-specific chains determine the specificity of the O antigens of a given serotype. O antigens are the immunodominant part of the lipopolysaccharide molecule in the intact bacterial cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)

Top Publications

  1. ncbi Biosynthesis and assembly of capsular polysaccharides in Escherichia coli
    Chris Whitfield
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada
    Annu Rev Biochem 75:39-68. 2006
  2. ncbi Biosynthesis of O-antigens: genes and pathways involved in nucleotide sugar precursor synthesis and O-antigen assembly
    Gabrielle Samuel
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW 2006, Australia
    Carbohydr Res 338:2503-19. 2003
  3. ncbi Bacterial polysaccharide synthesis and gene nomenclature
    P R Reeves
    Dept of Microbiology, University of Sydney, NSW, Australia
    Trends Microbiol 4:495-503. 1996
  4. ncbi Structure, assembly and regulation of expression of capsules in Escherichia coli
    C Whitfield
    Department of Microbiology, University of Guelph, Ontario, Canada
    Mol Microbiol 31:1307-19. 1999
  5. ncbi Regulation of Salmonella typhimurium lipopolysaccharide O antigen chain length is required for virulence; identification of FepE as a second Wzz
    Gerald L Murray
    Department of Molecular Biosciences, The University of Adelaide, Adelaide, South Australia, Australia, 5005
    Mol Microbiol 47:1395-406. 2003
  6. ncbi Structure and genetics of Shigella O antigens
    Bin Liu
    TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China
    FEMS Microbiol Rev 32:627-53. 2008
  7. ncbi Detection of O antigens in Escherichia coli
    Chitrita Debroy
    E coli Reference Center, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, USA
    Anim Health Res Rev 12:169-85. 2011
  8. pmc Regulation by a novel protein of the bimodal distribution of lipopolysaccharide in the outer membrane of Escherichia coli
    R A Batchelor
    Department of Microbiology and Immunology, Baylor College of Medicine, Texas Medical Center, Houston 77030
    J Bacteriol 173:5699-704. 1991
  9. pmc Altering the length of the lipopolysaccharide O antigen has an impact on the interaction of Salmonella enterica serovar Typhimurium with macrophages and complement
    Gerald L Murray
    Australian Bacterial Pathogenesis Program, Discipline of Microbiology and Immunology, School of Molecular and Biomedical Sciences, The University of Adelaide, Adelaide, Australia 5005
    J Bacteriol 188:2735-9. 2006
  10. pmc An O-antigen processing function for Wzx (RfbX): a promising candidate for O-unit flippase
    D Liu
    Department of Microbiology, University of Sydney, New South Wales, Australia
    J Bacteriol 178:2102-7. 1996

Detail Information

Publications315 found, 100 shown here

  1. ncbi Biosynthesis and assembly of capsular polysaccharides in Escherichia coli
    Chris Whitfield
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada
    Annu Rev Biochem 75:39-68. 2006
    ..These systems have an impact on both a general understanding of membrane trafficking in bacteria and on bacterial pathogenesis...
  2. ncbi Biosynthesis of O-antigens: genes and pathways involved in nucleotide sugar precursor synthesis and O-antigen assembly
    Gabrielle Samuel
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW 2006, Australia
    Carbohydr Res 338:2503-19. 2003
    ..This review is a summary of the current knowledge on these three groups of genes that comprise the O-antigen gene clusters, focusing on the most extensively studied E. coli and S. enterica gene clusters...
  3. ncbi Bacterial polysaccharide synthesis and gene nomenclature
    P R Reeves
    Dept of Microbiology, University of Sydney, NSW, Australia
    Trends Microbiol 4:495-503. 1996
    ..We propose a scheme applicable to all species that distinguishes different classes of genes, provides a single name for all genes of a given function and greatly facilitates comparative studies...
  4. ncbi Structure, assembly and regulation of expression of capsules in Escherichia coli
    C Whitfield
    Department of Microbiology, University of Guelph, Ontario, Canada
    Mol Microbiol 31:1307-19. 1999
    ..coli into four distinct groups...
  5. ncbi Regulation of Salmonella typhimurium lipopolysaccharide O antigen chain length is required for virulence; identification of FepE as a second Wzz
    Gerald L Murray
    Department of Molecular Biosciences, The University of Adelaide, Adelaide, South Australia, Australia, 5005
    Mol Microbiol 47:1395-406. 2003
    ..The presence of wzzfepE homologues in the genomic sequences of strains of Escherichia coli, Shigella flexneri and multiple serovars of Salmonella suggests that bimodality of LPS Oag is a common phenomenon in the Enterobacteriaceae...
  6. ncbi Structure and genetics of Shigella O antigens
    Bin Liu
    TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China
    FEMS Microbiol Rev 32:627-53. 2008
    This review covers the O antigens of the 46 serotypes of Shigella, but those of most Shigella flexneri are variants of one basic structure, leaving 34 Shigella distinct O antigens to review, together with their gene clusters...
  7. ncbi Detection of O antigens in Escherichia coli
    Chitrita Debroy
    E coli Reference Center, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, USA
    Anim Health Res Rev 12:169-85. 2011
    Lipopolysaccharide on the surface of Escherichia coli constitutes the O antigens which are important virulence factors that are targets of both the innate and adaptive immune systems and play a major role in host-pathogen interactions...
  8. pmc Regulation by a novel protein of the bimodal distribution of lipopolysaccharide in the outer membrane of Escherichia coli
    R A Batchelor
    Department of Microbiology and Immunology, Baylor College of Medicine, Texas Medical Center, Houston 77030
    J Bacteriol 173:5699-704. 1991
    ..Mutation of the gene encoding this protein destroyed its ability to regulate O-chain distribution. We propose to call this regulator gene rol, for regulator of O length...
  9. pmc Altering the length of the lipopolysaccharide O antigen has an impact on the interaction of Salmonella enterica serovar Typhimurium with macrophages and complement
    Gerald L Murray
    Australian Bacterial Pathogenesis Program, Discipline of Microbiology and Immunology, School of Molecular and Biomedical Sciences, The University of Adelaide, Adelaide, Australia 5005
    J Bacteriol 188:2735-9. 2006
    ..No evidence was found to suggest that modifying the length of the O-antigen polymer affected expression of the O1, O4, or O5 antigenic factors...
  10. pmc An O-antigen processing function for Wzx (RfbX): a promising candidate for O-unit flippase
    D Liu
    Department of Microbiology, University of Sydney, New South Wales, Australia
    J Bacteriol 178:2102-7. 1996
    ....
  11. ncbi The structures of Escherichia coli O-polysaccharide antigens
    Roland Stenutz
    Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Stockholm, Sweden
    FEMS Microbiol Rev 30:382-403. 2006
    ..In addition, results pertinent to the biosynthesis of the repeating units of O-antigens are discussed. The ECODAB is accessible to the scientific community at http://www.casper.organ.su.se/ECODAB/...
  12. pmc Molecular analysis of the contribution of the capsular polysaccharide and the lipopolysaccharide O side chain to the virulence of Klebsiella pneumoniae in a murine model of pneumonia
    Guadalupe Cortés
    Unidad de Investigacion, Hospital Universitario Son Dureta, Palma de Mallorca, Spain
    Infect Immun 70:2583-90. 2002
    ..We conclude that CPS, but not LPS O side chain, is essential for Klebsiella pneumonia because it modulates the deposition of C3 and protects the microorganisms against human AM phagocytosis...
  13. ncbi O-antigen structural variation: mechanisms and possible roles in animal/plant-microbe interactions
    Inge Lerouge
    Centre of Microbial and Plant Genetics, Katholieke Universtiteit Leuven, Kasteelpark Arenberg 20, B 3001, Heverlee, Belgium
    FEMS Microbiol Rev 26:17-47. 2002
    ..However, the genetic mechanisms by which the LPS epitope changes are regulated remain unclear. Finally, the possible roles of O-antigen variations in symbiosis will be discussed...
  14. ncbi Genetic modulation of Shigella flexneri 2a lipopolysaccharide O antigen modal chain length reveals that it has been optimized for virulence
    Renato Morona
    Department of Molecular Biosciences, University of Adelaide, Adelaide, South Australia, Australia, 5005
    Microbiology 149:925-39. 2003
    ..It is suggested that S. flexneri 2a has evolved to synthesize LPS with two Oag modal chain lengths, as S-type Oag chains allow IcsA to function in ABM in the presence of VL-type Oag chains that confer resistance to serum...
  15. ncbi Optimization of virulence functions through glucosylation of Shigella LPS
    Nicholas P West
    Centre for Molecular Microbiology and Infection, Department of Infectious Diseases, Faculty of Medicine, Flowers Building, Imperial College London, London SW7 2AZ, UK
    Science 307:1313-7. 2005
    ..Thus, LPS glucosylation promotes bacterial invasion and evasion of innate immunity, which may have contributed to the emergence of serotype diversity in Shigella...
  16. ncbi Type IV O antigen modification genes in the genome of Shigella flexneri NCTC 8296
    M M Adams
    Division of Biochemistry and Molecular Biology, School of Life Sciences, Faculty of Science, The Australian National University, Canberra, ACT 0200, Australia
    Microbiology 147:851-60. 2001
    ..Immunogold labelling and phage sensitivity indicated the presence of both serotype Y and serotype 4a O antigens on the cell surface of the resulting recombinant SFL124 strains, suggesting that only partial serotype ..
  17. pmc Defective O-antigen polymerization in tolA and pal mutants of Escherichia coli in response to extracytoplasmic stress
    Enrique D Vines
    Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada
    J Bacteriol 187:3359-68. 2005
    ..enterica O:54 LPS. Therefore, TolA and Pal are required for the correct surface expression of O antigens that are assembled in a wzy (polymerase)-dependent manner (like those of E...
  18. pmc O-antigen and virulence profiling of shiga toxin-producing Escherichia coli by a rapid and cost-effective DNA microarray colorimetric method
    Beatriz Quiñones
    Produce Safety and Microbiology Research Unit, Western Regional Research Center, U S Department of Agriculture Agricultural Research Service Albany, CA, USA
    Front Cell Infect Microbiol 2:61. 2012
    ....
  19. pmc Mutagenesis and chemical cross-linking suggest that Wzz dimer stability and oligomerization affect lipopolysaccharide O-antigen modal chain length control
    Magdalene Papadopoulos
    Discipline of Microbiology and Immunology, School of Molecular and Biomedical Science, University of Adelaide, Adelaide, South Australia, Australia 5005
    J Bacteriol 192:3385-93. 2010
    ..These data suggest that the ability to produce stable dimers may be important in determining Oag modal chain length...
  20. pmc Dual conserved periplasmic loops possess essential charge characteristics that support a catch-and-release mechanism of O-antigen polymerization by Wzy in Pseudomonas aeruginosa PAO1
    Salim T Islam
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada
    J Biol Chem 286:20600-5. 2011
    ..These observations support the proposed role of each PL in a catch-and-release mechanism for Wzy-mediated O-antigen polymerization...
  21. pmc Extensive variation in the O-antigen gene cluster within one Salmonella enterica serogroup reveals an unexpected complex history
    Lei Wang
    Department of Microbiology, The University of Sydney, Sydney, New South Wales 2006, Australia
    J Bacteriol 184:1669-77. 2002
    ..Two of the forms lacking a functional wzy(alpha(1-6)) gene have the wzy(alpha(1-2)) gene first described for strain LT2 as rfc, whereas for the third the wzy gene has not been located...
  22. ncbi Detection by multiplex real-time polymerase chain reaction assays and isolation of Shiga toxin-producing Escherichia coli serogroups O26, O45, O103, O111, O121, and O145 in ground beef
    Pina M Fratamico
    Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA
    Foodborne Pathog Dis 8:601-7. 2011
    ..This work provides a method for detection and isolation in ground beef and potentially other foods of non-O157 STEC of major public health concern...
  23. pmc The genetic and molecular basis of O-antigenic diversity in Burkholderia pseudomallei lipopolysaccharide
    Apichai Tuanyok
    Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona, USA
    PLoS Negl Trop Dis 6:e1453. 2012
    ..We postulate that the diversity of LPS may correlate with differential immunopathogenicity and virulence among B. pseudomallei strains...
  24. ncbi Micromethods for the characterization of lipid A-core and O-antigen lipopolysaccharide
    Cristina L Marolda
    Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada
    Methods Mol Biol 347:237-52. 2006
    ..This chapter describes a collection of methods routinely used in our laboratory for the characterization of LPS in Escherichia coli and other bacteria...
  25. pmc Relationships of the Escherichia coli O157, O111, and O55 O-antigen gene clusters with those of Salmonella enterica and Citrobacter freundii, which express identical O antigens
    Gabrielle Samuel
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    J Bacteriol 186:6536-43. 2004
    ..For both the S. enterica O30-C. freundii F90-E. coli O157 group and the S. enterica O50-E. coli O55 group of O antigens, the gene clusters have identical or nearly identical organizations...
  26. ncbi Pseudomonas aeruginosa O-antigen chain length is determined before ligation to lipid A core
    Craig Daniels
    Department of Microbiology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
    Environ Microbiol 4:883-97. 2002
    ..We have shown an association between the Wzz proteins and O-antigen polymer chains using immunoprecipitation with anti-O5 O-antigen monoclonal antibody MF15-4. Both Wzz1 and Wzz2 could be co-precipitated with O5 polymer...
  27. pmc Porphyromonas gingivalis galE is involved in lipopolysaccharide O-antigen synthesis and biofilm formation
    Ryoma Nakao
    Department of Bacteriology, National Institute of Infectious Diseases, 1 23 1, Toyama, Shinjuku ku, Tokyo, Japan 162 8640
    Infect Immun 74:6145-53. 2006
    ..gingivalis galE gene and virulence phenotypes such as biofilm formation may provide clues for understanding the mechanism of pathogenicity in periodontal disease...
  28. ncbi Micro-array for the identification of Shiga toxin-producing Escherichia coli (STEC) seropathotypes associated with Hemorrhagic Colitis and Hemolytic Uremic Syndrome in humans
    Marie Bugarel
    Afssa French Food Safety Agency, Laboratory for Study and Research on Food Quality and Processes LERQAP, 23 Av du Général De Gaulle, Fr 94706 Maisons Alfort, France
    Int J Food Microbiol 142:318-29. 2010
    ..coli (EHEC) serotypes. The genes selected for determination of the O antigens (rfbE(O157), wzx(O26), wzx(O103), wbd1(O111), ihp1(O145), wzx(O121), wzy(O113), wzy(O91), wzx(O104), wzy(O118), ..
  29. pmc Molecular, genetic, and topological characterization of O-antigen chain length regulation in Shigella flexneri
    R Morona
    Department of Microbiology and Immunology, University of Adelaide, Australia
    J Bacteriol 177:1059-68. 1995
    ..flexneri by insertion of a kanamycin resistance cartridge. The resulting strains produced LPS with O antigens of nonmodal chain length, thereby confirming the function of the rol gene product...
  30. ncbi Serotype-converting bacteriophages and O-antigen modification in Shigella flexneri
    G E Allison
    Divn of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, Canberra, ACT, 0200, Australia
    Trends Microbiol 8:17-23. 2000
    ..Several serotype-converting phages have been isolated and preliminary characterization has identified the genes involved in O-antigen modification, and has also provided insight into the molecular biology of these phages...
  31. pmc Identification of a bile-induced exopolysaccharide required for Salmonella biofilm formation on gallstone surfaces
    Robert W Crawford
    Center for Microbial Interface Biology and Department of Molecular Virology, Immunology, and Medical Genetics, The Ohio State University, Columbus, Ohio 43210, USA
    Infect Immun 76:5341-9. 2008
    ..These studies provide new therapeutic targets for preventing asymptomatic serovar Typhi gallbladder carriage...
  32. pmc Phase variation controls expression of Salmonella lipopolysaccharide modification genes by a DNA methylation-dependent mechanism
    S E Broadbent
    Centre for Immunology and Infection, Hull York Medical School and the Department of Biology, University of York, York, UK
    Mol Microbiol 77:337-53. 2010
    ..Most Salmonella gtr operons share the key regulatory elements that are identified here as essential for this epigenetic phase variation...
  33. pmc Effect of the O-antigen length of lipopolysaccharide on the functions of Type III secretion systems in Salmonella enterica
    Stefanie U Hölzer
    Mikrobiologisches Institut, Universitatsklinikum Erlangen, Erlangen, Germany
    Infect Immun 77:5458-70. 2009
    ..These observations indicate that the architecture of the outer membrane of Salmonella is balanced to permit sufficient T3SS function but also to confer optimal protection against antimicrobial defense mechanisms...
  34. pmc Burkholderia thailandensis oacA mutants facilitate the expression of Burkholderia mallei-like O polysaccharides
    Paul J Brett
    Department of Microbiology and Immunology, University of South Alabama, 5851 USA Drive North, Mobile, AL 36688, USA
    Infect Immun 79:961-9. 2011
    ..thailandensis OPS antigens and that ZT0715 may provide a safe and cost-effective source of B. mallei-like OPS to facilitate the synthesis of glanders subunit vaccine candidates...
  35. pmc Identification of a second lipopolysaccharide in Porphyromonas gingivalis W50
    Minnie Rangarajan
    Centre for Infectious Disease, Institute of Cell and Molecular Science, Barts and The London Queen Mary s School of Medicine and Dentistry, 4 Newark Street, London E1 2AT, United Kingdom
    J Bacteriol 190:2920-32. 2008
    ..gingivalis has dual specificity for both O-antigen and APS repeating units...
  36. ncbi Bacterial glycans: key mediators of diverse host immune responses
    Laurie E Comstock
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Cell 126:847-50. 2006
    ..Here, we discuss the diverse interactions between bacterial glycans and the host immune system...
  37. ncbi WaaL of Pseudomonas aeruginosa utilizes ATP in in vitro ligation of O antigen onto lipid A-core
    Priyanka D Abeyrathne
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada
    Mol Microbiol 65:1345-59. 2007
    ..A waaL mutant of P. aeruginosa could not be cross-complemented by waaL of Escherichia coli, which suggested that each of these proteins has specificity for its cognate core oligosaccharide...
  38. ncbi The PmrA/PmrB and RcsC/YojN/RcsB systems control expression of the Salmonella O-antigen chain length determinant
    Monica A Delgado
    Department of Molecular Microbiology, Washington University School of Medicine, 660 S Euclid, St Louis, MO 63110, USA
    Mol Microbiol 60:39-50. 2006
    ..That multiple regulatory systems control wzzst expression suggests that the Wzzst protein is required under different environmental conditions...
  39. ncbi Export of O-specific lipopolysaccharide
    Miguel A Valvano
    Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1 Canada
    Front Biosci 8:s452-71. 2003
    ..side of the plasma membrane as well as several different translocation pathways that deliver the nascent O antigens in a glycolipid form to the periplasmic side of the plasma membrane...
  40. pmc A novel glucosyltransferase involved in O-antigen modification of Shigella flexneri serotype 1c
    Robert M Stagg
    Biochemistry and Molecular Biology, Research School of Biology, The Australian National University, Canberra, ACT 0200, Australia
    J Bacteriol 191:6612-7. 2009
    ..flexneri from outside the species. Analysis of surrounding sequence suggests that the gtrIC cluster arrived via a novel bacteriophage that was subsequently rendered nonfunctional by a series of insertion events...
  41. pmc Structural and genetic analyses of O polysaccharide from Actinobacillus actinomycetemcomitans serotype f
    J B Kaplan
    Department of Oral Pathology, Biology and Diagnostic Sciences, New Jersey Dental School, Newark, New Jersey 07103 2714, USA
    Infect Immun 69:5375-84. 2001
    ..The finding of an A. actinomycetemcomitans serotype showing serological cross-reactivity with anti-serotype b-specific antiserum suggests that a reevaluation of strains previously classified as serotype b may be warranted...
  42. pmc Organization of Escherichia coli O157 O antigen gene cluster and identification of its specific genes
    L Wang
    Department of Microbiology, The University of Sydney, Sydney, New South Wales 2006, Australia
    Infect Immun 66:3545-51. 1998
    ..coli or, once flanking genes are identified, clones of any gram-negative bacterium...
  43. ncbi Structure of the O-polysaccharide of Aeromonas hydrophila O:34; a case of random O-acetylation of 6-deoxy-L-talose
    Yuriy A Knirel
    N D Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, Moscow 119991, Russia
    Carbohydr Res 337:1381-6. 2002
    ....
  44. ncbi Mice vaccinated with the O-antigen of Francisella tularensis LVS lipopolysaccharide conjugated to bovine serum albumin develop varying degrees of protective immunity against systemic or aerosol challenge with virulent type A and type B strains of the path
    J Wayne Conlan
    National Research Council Canada, Institute for Biological Sciences, Ont, K1A OR6, Ottawa, Canada
    Vaccine 20:3465-71. 2002
    ..The results suggest that the O-antigen of F. tularensis could be considered as a potential component of a subunit vaccine against type B, but not type A strains of F. tularensis...
  45. ncbi Polysaccharides and virulence of Burkholderia pseudomallei
    M Sarkar-Tyson
    Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, UK
    J Med Microbiol 56:1005-10. 2007
    ..However, immunization with killed polysaccharide mutant strains conferred different degrees of protection, demonstrating the immunological importance of the polysaccharide clusters on the surface of B. pseudomallei...
  46. pmc The O antigen is a critical antigen for the development of a protective immune response to Bordetella parapertussis
    Xuqing Zhang
    Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, 115 Henning Building, University Park, PA 16802, USA
    Infect Immun 77:5050-8. 2009
    ..parapertussis. These data suggest that the O antigen is a critical protective antigen of B. parapertussis and its inclusion can substantially improve whooping cough vaccine efficacy against this pathogen...
  47. ncbi 'Form variation' of the O12 antigen is critical for persistence of Salmonella Typhimurium in the murine intestine
    Lydia M Bogomolnaya
    Department of Microbial and Molecular Pathogenesis, College of Medicine, Texas A and M University System Health Science Center, College Station, TX 77843 1114, USA
    Mol Microbiol 70:1105-19. 2008
    ..Our data suggest that the 12-2 antigen is a S. enterica subspecies I-specific LPS modification that enhances long-term intestinal colonization, and is in contrast to the role of O-antigen variation described for Shigella...
  48. pmc Genetic modification of the O-polysaccharide of Francisella tularensis results in an avirulent live attenuated vaccine
    Tae Hyun Kim
    Department of Microbiology and Molecular Genetics, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Infect Dis 205:1056-65. 2012
    ..Francisella tularensis, the causative agent of tularemia, is a highly virulent microbe. One significant virulence factor of F. tularensis is the O-polysaccharide (O-PS) portion of the organism's lipopolysaccharide...
  49. pmc O-polysaccharide glycosylation is required for stability and function of the collagen adhesin EmaA of Aggregatibacter actinomycetemcomitans
    Gaoyan Tang
    Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, Vermont, USA
    Infect Immun 80:2868-77. 2012
    ..The glycan modification of EmaA appears to be required for collagen binding activity and protection of the protein against degradation by proteolytic enzymes...
  50. ncbi The Wzx translocases for Salmonella enterica O-antigen processing have unexpected serotype specificity
    Yaoqin Hong
    School of Molecular Bioscience, The University of Sydney, Sydney, NSW, Australia
    Mol Microbiol 84:620-30. 2012
    ..widely thought to be specific only for the first sugar of the repeat unit, despite extensive variation in both O antigens and Wzx translocases. However, we found for S...
  51. ncbi Polysaccharide co-polymerases: the enigmatic conductors of the O-antigen assembly orchestra
    Sergei Kalynych
    Department of Biochemistry, McGill University, Montreal, Quebec H3G0B1, Canada
    Protein Eng Des Sel 25:797-802. 2012
    ..Here we review the current knowledge of structure and function of these polysaccharide length regulators...
  52. ncbi Structural analysis of the O-antigen polysaccharide from Escherichia coli O152
    Ulrika Olsson
    Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, S 106 91 Stockholm, Sweden
    Carbohydr Res 340:167-71. 2005
    ..coli O173. The cross-reactivity between E. coli O152 and E. coli O3 may be explained by structural similarities in the branching region of their O-antigen polysaccharides...
  53. pmc Comparison of O-antigen gene clusters of Escherichia coli (Shigella) sonnei and Plesiomonas shigelloides O17: sonnei gained its current plasmid-borne O-antigen genes from P. shigelloides in a recent event
    J G Shepherd
    Department of Microbiology, The University of Sydney, Sydney, New South Wales 2006, Australia
    Infect Immun 68:6056-61. 2000
    ..By sequencing the chromosomal O-antigen gene cluster of P. shigelloides O17 and comparing it with that of Sonnei, we showed that Sonnei gained its O-antigen genes recently...
  54. pmc C-terminal half of Salmonella enterica WbaP (RfbP) is the galactosyl-1-phosphate transferase domain catalyzing the first step of O-antigen synthesis
    L Wang
    Department of Microbiology, The University of Sydney, New South Wales, Australia
    J Bacteriol 178:2598-604. 1996
    ..We now propose that the T block operates prior to the flippase function, probably at the release of undecaprenyl pyrophosphate-linked galactose from WbaP...
  55. ncbi A rapid procedure for the detection and isolation of enterohaemorrhagic Escherichia coli (EHEC) serogroup O26, O103, O111, O118, O121, O145 and O157 strains and the aggregative EHEC O104:H4 strain from ready-to-eat vegetables
    Markus Tzschoppe
    National Reference Laboratory for Escherichia coli, Unit 41 Microbial Toxins, Germany
    Int J Food Microbiol 152:19-30. 2012
    ..Aggregative EHEC O104:H4 could be detected and isolated from a sample of sprouted seeds which was suspected as vector of transmission of EHEC O104 to humans...
  56. pmc Salmonella enterica serovar enteritidis core O polysaccharide conjugated to H:g,m flagellin as a candidate vaccine for protection against invasive infection with S. enteritidis
    Raphael Simon
    Center for Vaccine Development, University of Maryland, Baltimore, Baltimore, MD 21201, USA
    Infect Immun 79:4240-9. 2011
    ..Mice immunized with flagellin alone, COPS-CRM₁₉₇, or COPS-flagellin conjugates were significantly protected from lethal challenge with wild-type S. Enteritidis (80 to 100% vaccine efficacy)...
  57. ncbi Lipopolysaccharide O-antigen promotes persistent murine bacteremia
    Joanelle Z Lugo
    Department of Emergency Medicine, University of Michigan, Ann Arbor, MI 48109, USA
    Shock 27:186-91. 2007
    ....
  58. ncbi Structural and genetic characterization of Escherichia coli O99 antigen
    Andrei V Perepelov
    N D Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
    FEMS Immunol Med Microbiol 57:80-7. 2009
    ..Our findings indicate that in E. coli O99, the synthesis and translocation of the O-antigen occurs by an ABC transporter-dependent process...
  59. ncbi Sequence of the Escherichia coli O26 O antigen gene cluster and identification of O26 specific genes
    Jocelyne M D'Souza
    Department of Microbiology, School of Molecular and Microbial Biosciences G08, University of Sydney, Sydney, NSW 2006, Australia
    Gene 297:123-7. 2002
    Escherichia coli associated with outbreaks of gastroenteritis and hemolytic uremic syndrome include clones with O antigens O157 and O111...
  60. pmc O antigen protects Bordetella parapertussis from complement
    Elizabeth M Goebel
    Department of Veterinary and Biomedical Sciences, Pennsylvania State University, 115 Henning Building, University Park, PA 16802, USA
    Infect Immun 76:1774-80. 2008
    ..These data indicate that O antigen enables B. parapertussis to efficiently colonize the lower respiratory tract by protecting against complement-mediated control and clearance...
  61. ncbi O antigen gene clusters of Yersinia pseudotuberculosis
    Peter R Reeves
    School of Molecular and Microbial Biosciences, University of Sydney, NSW2006, Australia
    Adv Exp Med Biol 529:199-206. 2003
  62. ncbi Sequence and analysis of the O antigen gene (rfb) cluster of Escherichia coli O111
    D A Bastin
    Department of Microbiology, University of Sydney, New South Wales, Australia
    Gene 164:17-23. 1995
    The O antigens found in Salmonella enterica (Se) and Escherichia coli (Ec) show a great deal of diversity, and only three structures are known to be common to both genera...
  63. ncbi Nonreducing terminal modifications determine the chain length of polymannose O antigens of Escherichia coli and couple chain termination to polymer export via an ATP-binding cassette transporter
    Bradley R Clarke
    Department of Microbiology, University of Guelph, Guelph, Ontario N1G 2W1, Canada
    J Biol Chem 279:35709-18. 2004
    The chain length of bacterial lipopolysaccharide O antigens is regulated to give a modal distribution that is critical for pathogenesis...
  64. pmc Flavonoid-inducible modifications to rhamnan O antigens are necessary for Rhizobium sp. strain NGR234-legume symbioses
    W J Broughton
    LBMPS, Universite de Geneve, Switzerland
    J Bacteriol 188:3654-63. 2006
    ..Mutation of either pathway reduces rhamnan production. Coordination of rhamnan synthesis with rhizobial release from infection threads is thus part of the symbiotic interaction...
  65. pmc Identification, characterization and immunogenicity of an O-antigen capsular polysaccharide of Francisella tularensis
    Michael A Apicella
    Department of Microbiology, University of Iowa, Iowa City, Iowa, United States of America
    PLoS ONE 5:e11060. 2010
    ..tularensis LVS. Active immunization of BALB/c mice with 10 microg of capsule showed a similar level of protection. These studies demonstrate that F. tularensis produces an O-antigen capsule that may be the basis of a future vaccine...
  66. pmc Post-assembly modification of Bordetella bronchiseptica O polysaccharide by a novel periplasmic enzyme encoded by wbmE
    Jerry D King
    Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, United Kingdom
    J Biol Chem 284:1474-83. 2009
    ....
  67. pmc Comparative genomics of the classical Bordetella subspecies: the evolution and exchange of virulence-associated diversity amongst closely related pathogens
    Jihye Park
    Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, USA
    BMC Genomics 13:545. 2012
    ..The compelling picture from previous comparisons of the three sequenced genomes was of genome degradation, with substantial loss of genome content (up to 24%) associated with adaptation to humans...
  68. pmc Phase 1 and phase 2 studies of Salmonella enterica serovar paratyphi A O-specific polysaccharide-tetanus toxoid conjugates in adults, teenagers, and 2- to 4-year-old children in Vietnam
    E Y Konadu
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2720, USA
    Infect Immun 68:1529-34. 2000
    ..0001); a second injection did not elicit a booster response. Representative sera from all groups had bactericidal activity that could be adsorbed by S. enterica serovar Paratyphi A LPS...
  69. ncbi Lipopolysaccharide heterogeneity in Brucella strains isolated from marine mammals
    Sylvie Baucheron
    Institut National de la Recherche Agronomique, Unité de Pathologie Aviaire et Parasitologie, Nouzilly, France
    Res Microbiol 153:277-80. 2002
    ..Thus, S-LPSs of the marine mammal isolates show heterogeneity with regard to their O-PS C epitope content and their average size...
  70. pmc Novel role of the lipopolysaccharide O1 side chain in ferric siderophore transport and virulence of Vibrio anguillarum
    Timothy J Welch
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University 3181 Sam Jackson Park Road, Portland, OR 97239, USA
    Infect Immun 73:5864-72. 2005
    ..anguillarum: serum resistance and anguibactin-mediated iron transport. These factors allow V. anguillarum to survive in serum and multiply in the iron-limiting milieu of the host vertebrate...
  71. ncbi Genomic comparison of the O-antigen biosynthesis gene clusters of Escherichia coli O55 strains belonging to three distinct lineages
    Atsushi Iguchi
    Division of Bioenvironmental Science, Frontier Science Research Center, University of Miyazaki, Miyazaki, Japan
    Microbiology 154:559-70. 2008
    ..coli lineages. The data provide new insights into the mechanisms involved in the generation of a wide variety of O-serotypes in Gram-negative bacteria...
  72. ncbi Putative O-antigen transport genes within the rfb region of Vibrio cholerae O1 are homologous to those for capsule transport
    P A Manning
    Department of Microbiology and Immunology, University of Adelaide, Australia
    Gene 158:1-7. 1995
    ..The function of RfbG is unknown, but it would appear to be a relatively hydrophilic protein and we can only speculate that it may be either a specific transferase or possibly the O-antigen polymerase...
  73. pmc Evidence for horizontal gene transfer of two antigenically distinct O antigens in Bordetella bronchiseptica
    Anne M Buboltz
    Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, 16802, USA
    Infect Immun 77:3249-57. 2009
    ..from strain RB50 (O1), 1289 (O2), or RB50Deltawbm (O antigen deficient), our data indicate that these O antigens do not confer cross-protection in vivo...
  74. ncbi O-antigen diversity and lateral transfer of the wbe region among Vibrio splendidus isolates
    Hans Wildschutte
    Civil and Environmental Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Environ Microbiol 12:2977-87. 2010
    ..Our results demonstrate O-antigen hyper-variability among these environmental strains and suggest that frequent lateral gene transfer generates wbe extensive diversity among V. splendidus and its close relatives...
  75. ncbi A new ethanolamine phosphate-containing variant of the O-antigen of Shigella flexneri type 4a
    Andrei V Perepelov
    N D Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation
    Carbohydr Res 344:1588-91. 2009
    ..Phosphorylation has not been hitherto reported in any S. flexneri O-antigen. Reinvestigation of the O-specific polysaccharide of S. flexneri type 4b showed that it is not phosphorylated and confirmed its structure established earlier...
  76. ncbi Membrane topology of the Salmonella enterica serovar Typhimurium Group B O-antigen translocase Wzx
    Monica M Cunneen
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW, Australia
    FEMS Microbiol Lett 287:76-84. 2008
    ..Enzyme activity of these, and full-length C-terminal fusion proteins, confirmed the 12-TMS topology for this Wzx, and also indicated that the C-terminus was located within the cytoplasm, which is consistent with the predicted topology...
  77. ncbi Molecular evolution of the GDP-mannose pathway genes (manB and manC) in Salmonella enterica
    S O Jensen
    Department of Microbiology (G08, University of Sydney, New South Wales 2006, Australia
    Microbiology 147:599-610. 2001
    ..pathway in Salmonella enterica was studied via sequencing manB and manC genes from 13 representative strains for O antigens containing mannose and/or sugar derivatives of GDP-D-mannose...
  78. pmc O-antigen-negative Salmonella enterica serovar Typhimurium is attenuated in intestinal colonization but elicits colitis in streptomycin-treated mice
    Karin Ilg
    Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, ETH Zurich, Zurich, Switzerland
    Infect Immun 77:2568-75. 2009
    ..Our experiments prove that the presence of complete LPS is not essential for in vitro invasion or for triggering acute colitis...
  79. ncbi The Klebsiella pneumoniae O2a antigen defines a second mechanism for O antigen ATP-binding cassette transporters
    Veronica Kos
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada
    J Biol Chem 284:2947-56. 2009
    ..ATP-binding cassette (ABC) transporter-dependent mechanism is responsible for the biosynthesis of polysaccharide O antigens in the lipopolysaccharides of many Gram-negative bacteria...
  80. ncbi The role of lipopolysaccharide moieties in macrophage response to Escherichia coli
    Katalin Eder
    Biological Research Center of Hungarian Academy of Sciences, Institute of Biochemistry, Temesvari krt 62, Szeged H 6726, Hungary
    Biochem Biophys Res Commun 389:46-51. 2009
    ..The most ancient part, lipid A is crucial in evoking immediate TNF release and activation of NF-kappaB. The O-antigen inhibits phagocytosis, leading to immune evasion...
  81. pmc The Klebsiella pneumoniae O antigen contributes to bacteremia and lethality during murine pneumonia
    Sunita Shankar-Sinha
    Department of Emergency Medicine, School of Medicine, University of Michigan, Ann Arbor, Michigan, USA
    Infect Immun 72:1423-30. 2004
    ..These results suggest that during murine pneumonia caused by K. pneumoniae, O antigen contributes to lethality by increasing the propensity for bacteremia and not by significantly changing the early course of intrapulmonary infection...
  82. pmc Structure-guided investigation of lipopolysaccharide O-antigen chain length regulators reveals regions critical for modal length control
    Sergei Kalynych
    Department of Biochemistry, McGill University, Montreal, Quebec, Canada
    J Bacteriol 193:3710-21. 2011
    ..Together, our data suggest that chain length determination depends on regions that likely contribute to stabilize a supramolecular complex...
  83. pmc Vibrio anguillarum resistance to rainbow trout (Oncorhynchus mykiss) serum: role of O-antigen structure of lipopolysaccharide
    H T Boesen
    Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Frederiksberg C, Denmark
    Infect Immun 67:294-301. 1999
    ..Strains grown in glucose-enriched medium became sensitive to rainbow trout serum killing, indicating that the high-molecular-weight O-antigen side chains prevented the activated complement from damaging the bacterium...
  84. ncbi Characterization of functional oligosaccharide mimics of the Shigella flexneri serotype 2a O-antigen: implications for the development of a chemically defined glycoconjugate vaccine
    Armelle Phalipon
    Unite de Pathogenie Microbienne Moleculaire, Institut National de la Santé et de la Recherche Médicale Unité 389
    J Immunol 176:1686-94. 2006
    ..A pentadecasaccharide representing three biological repeating units was identified as a potential candidate for further development of a chemically defined glycoconjugate vaccine against S. flexneri 2a infection...
  85. ncbi The wbiA locus is required for the 2-O-acetylation of lipopolysaccharides expressed by Burkholderia pseudomallei and Burkholderia thailandensis
    Paul J Brett
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, 3330 Hospital Drive NW, T2N 4N1, Calgary, AB, Canada
    FEMS Microbiol Lett 218:323-8. 2003
    ....
  86. pmc Protection of mice against Salmonella typhimurium with an O-specific polysaccharide-protein conjugate vaccine
    D C Watson
    Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, Bethesda, Maryland 20892
    Infect Immun 60:4679-86. 1992
    ..These results indicate that an O-SP-TT conjugate, when given by a route and formulation acceptable for human use, protects mice against challenge with S. typhimurium...
  87. ncbi Inducible serum resistance in Salmonella typhimurium is dependent on wzz(fepE)-regulated very long O antigen chains
    Gerald L Murray
    Australian Bacterial Pathogenesis Program, Discipline of Microbiology and Immunology, School of Molecular and Biomedical Science, The University of Adelaide, Adelaide 5005, Australia
    Microbes Infect 7:1296-304. 2005
    ..Since systemic infection occurs in a small but significant percentage of human non-typhoid salmonellosis, the phenomenon identified in this study may be significant during the bacteraemic phase of infection...
  88. pmc Serotype 1a O-antigen modification: molecular characterization of the genes involved and their novel organization in the Shigella flexneri chromosome
    P Adhikari
    Division of Biochemistry and Molecular Biology, Faculty of Science, The Australian National University, Canberra, ACT 0200, Australia
    J Bacteriol 181:4711-8. 1999
    ..6-kb region of the Y53 chromosome is unique and suggests that the serotype conversion genes were originally brought into the host by a bacteriophage. Several features of this region are also characteristic of pathogenicity islands...
  89. pmc Membrane topology and identification of critical amino acid residues in the Wzx O-antigen translocase from Escherichia coli O157:H4
    Cristina L Marolda
    Center for Human Immunology, Siebens Drake Research Institute, Departments of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada
    J Bacteriol 192:6160-71. 2010
    ..We propose that the functional requirement of charged residues at both sides of the membrane and in two TM helices could be important to allow the passage of the Und-PP-linked saccharide substrate across the membrane...
  90. ncbi Growth-phase regulation of lipopolysaccharide O-antigen chain length influences serum resistance in serovars of Salmonella
    Denisse Bravo
    Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Quimicas y Farmaceuticas, Universidad de Chile, PO Box 174 Correo 22, Santiago, Chile
    J Med Microbiol 57:938-46. 2008
    ..In contrast to the relevant role for high-molecular-mass OAg molecules, the presence of Vi antigen did not contribute to serum resistance of clinical isolates of serovar Typhi...
  91. ncbi The biosynthesis and biological role of lipopolysaccharide O-antigens of pathogenic Yersiniae
    Mikael Skurnik
    Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki and Helsinki University Central Hospital Laboratory Diagnostics, P O Box 63, FIN 00014 Helsinki, Finland
    Carbohydr Res 338:2521-9. 2003
    ....
  92. ncbi New rapid test for paratyphoid a fever: usefulness, cross-detection, and solution
    Frankie C H Tam
    Clinical Immunology Unit, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong
    Diagn Microbiol Infect Dis 62:142-50. 2008
    ....
  93. ncbi Lipopolysaccharide O antigen status of Yersinia enterocolitica O:8 is essential for virulence and absence of O antigen affects the expression of other Yersinia virulence factors
    José Antonio Bengoechea
    Unidad de Investigación and Institut Universitari d Investigacions en Ciències de la Salut, Hospital Son Dureta, Palma de Mallorca, Spain
    Mol Microbiol 52:451-69. 2004
    ..Together these findings suggest that the absence of O antigen in the outer membrane of Yersinia either directly or indirectly, for example through a cellular or membrane stress, could act as a regulatory signal...
  94. pmc Presence or absence of lipopolysaccharide O antigens affects type III secretion by Pseudomonas aeruginosa
    D K Augustin
    Biology Department, California State University, San Francisco, CA 94142, USA
    J Bacteriol 189:2203-9. 2007
    ..These results suggest the existence of a cooperative association between LPS O-antigen structure and the TTSS in both laboratory and clinical isolates of P. aeruginosa...
  95. ncbi Repeat unit polysaccharides of bacteria: a model for polymerization resembling that of ribosomes and fatty acid synthetase, with a novel mechanism for determining chain length
    D A Bastin
    Department of Microbiology, University of Sydney, NSW, Australia
    Mol Microbiol 7:725-34. 1993
    ..The CLD is not O-antigen or species-specific but the modal value does depend on the source of the cld gene...
  96. pmc Characterization of the O-antigen polymerase (Wzy) of Francisella tularensis
    Tae Hyun Kim
    Department of Microbiology and Molecular Genetics, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 285:27839-49. 2010
    ..Topology models indicate that these amino acids most likely lie in close proximity on the bacterial surface...
  97. pmc Yersinia enterocolitica serum resistance proteins YadA and ail bind the complement regulator C4b-binding protein
    Vesa Kirjavainen
    Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki, Finland
    PLoS Pathog 4:e1000140. 2008
    ..These findings show that Y. enterocolitica uses two proteins, YadA and Ail, to bind C4bp. Binding of C4bp could help Y. enterocolitica to evade complement-mediated clearance in the human host...
  98. ncbi The role of cellulose and O-antigen capsule in the colonization of plants by Salmonella enterica
    Jeri D Barak
    Produce Safety and Microbiology Research Unit, United States Department of Agriculture Agricultural Research Service, Albany, CA 94710, USA
    Mol Plant Microbe Interact 20:1083-91. 2007
    ..Outside of animal hosts, S. enterica appears to rely on an arsenal of adhesins to persist on plants, which can act as vectors and perpetuate public health concerns...
  99. ncbi The activity of a putative polyisoprenol-linked sugar translocase (Wzx) involved in Escherichia coli O antigen assembly is independent of the chemical structure of the O repeat
    M F Feldman
    Instituto de Investigaciones Bioquimicas Fundacion Campomar, Buenos Aires, Argentina
    J Biol Chem 274:35129-38. 1999
    ....
  100. pmc Development of PCR assays targeting genes in O-antigen gene clusters for detection and identification of Escherichia coli O45 and O55 serogroups
    Chitrita Debroy
    Gastroenteric Disease Center, The Pennsylvania State University, Wiley Lab, Wiley Lane, University Park, PA 16802, USA
    Appl Environ Microbiol 71:4919-24. 2005
    ..coli O55 wzx and wzy genes were used to detect the organisms in fecal samples spiked at levels of 10(6) and 10(8) CFU/0.2 g feces. Thus, the PCR assays can be used to detect and identify E. coli serogroups O45 and O55...
  101. pmc Evasion of complement-mediated lysis and complement C3 deposition are regulated by Francisella tularensis lipopolysaccharide O antigen
    Corey D Clay
    Department of Internal Medicine, Ohio State University, Columbus, OH 43210, USA
    J Immunol 181:5568-78. 2008
    ....

Research Grants62

  1. Mechanisms of adaptive immunity to P. aeruginosa in the lung
    Gregory P Priebe; Fiscal Year: 2013
    ..Finally, we will assess whether expression of heterologous O antigens by the vaccine or changes in its LPS lipid A acylation pattern can enhance its effectiveness...
  2. X-ray Studies of Sugar-Modifying Enzymes
    Hazel Holden; Fiscal Year: 2013
    ....
  3. Phospholipid flip-flop in biogenic membranes
    Anant K Menon; Fiscal Year: 2013
    ..coli lipopolysaccharide. We are interested in identifying the transport proteins that catalyze lipid flipping in yeast and mammals and understanding how they work. ..
  4. LIPID A MODIFICATION SYSTEMS IN GRAM-NEGATIVE BACTERIA
    Pei Zhou; Fiscal Year: 2012
    ....
  5. GENETICS AND VIRULENCE OF PNEUMOCOCCAL CAPSULAR TYPES
    JANET L YOTHER; Fiscal Year: 2012
    ..By understanding the mechanisms involved in this process, we may be better able to develop means for treating and preventing these infections. ..
  6. Research and Development of a Novel System to Produce Polysaccharide Conjugate Va
    Peng George Wang; Fiscal Year: 2013
    ....
  7. BIOSYNTHESIS AND FUNCTION OF LIPOPOLYSACCHARIDES
    Pei Zhou; Fiscal Year: 2013
    ....
  8. A Novel Surface Sensing Pathway in Proteus mirabilis
    Philip N Rather; Fiscal Year: 2013
    ..To address this gap, RNA-Seq will be used to identify genes that are specifically regulated by the response regulator RcsB. ..
  9. Live Attenuated Recombinant Bacterial Delivery of Polysaccharide Vaccine Antigens
    Joanna B Goldberg; Fiscal Year: 2010
    ..conjugate vaccines including subtype specificity and subtype inhibition, and the exclusion of acid-liable O antigens. The specific aims of this grant are to (1) determine the basis for protective immunity to P...
  10. Mass Spectrometric Characterization of Lipopolysaccharides
    Jennifer S Brodbelt; Fiscal Year: 2013
    ..Specific biological problems include correlation of the structura changes of lipid A with the inflammatory response, unraveling the biosynthetic pathway of LPS, and elucidating the mechanism of resistance to antimicrobial peptides. ..
  11. Crystallization of outer membrane proteins for export of polysaccharide capsule
    Mark A Saper; Fiscal Year: 2012
    ....
  12. O-Specific Polysaccharide Responses and Cholera
    Edward T Ryan; Fiscal Year: 2013
    ..abstract_text> ..
  13. Bacterial Surface Structures of Helicobacter Pylori and Campylobacter Jejuni
    MICHAEL STEPHEN TRENT; Fiscal Year: 2013
    ..Bacteria modify their LPS structure which directly impacts disease. This proposal will help determine how bacteria associated with human disease modify their LPS structure possibly leading to novel therapies. ..
  14. The Outer Surface of Vibrio cholerae
    MICHAEL STEPHEN TRENT; Fiscal Year: 2012
    ..cholerae lipid A species;(ii) environmental regulation of V. cholerae lipid A structure;(iii) enzymatic modification of V. cholerae lipid A;and (iv) Toll-like receptor mediated immune activation by V. cholerae LPS. ..
  15. Remodeling Salmonella outer membrane for vaccine development
    Qingke Kong; Fiscal Year: 2013
    ..The success of this project could lead to efficacious RASV with broad spectrum protection against enteric bacteria for human use. ..
  16. Development of O Antigen-based Vaccines Against Q Fever
    Guoquan Zhang; Fiscal Year: 2013
    ..burnetii infection, which will provide novel evidence to prove the concept that C. burnetii O antigen-based vaccines can confer protective immunity against Q fever. ..
  17. Bacteriolytic phage enzymes as novel antibacterials against Yersinia pestis
    Ian Molineux; Fiscal Year: 2009
    ..pestis infections. These in vivo studies will parallel those involving lysins using the mouse model of plague infection. ..
  18. Genome Evolution in Pathogens: The roles of predator-prey interactions
    Jeffrey G Lawrence; Fiscal Year: 2010
    ....
  19. Genetics and biosynthesis of an O antigen essential for symbiosis
    KENNETH D NOEL; Fiscal Year: 2010
    ..It also is important in other interactions with animal and plant hosts. This property is a type of carbohydrate chain on the surfaces of many bacteria. The question addressed in this work is how this chain is synthesized. ..
  20. Production of Polysaccharide-Protein Conjugates as Vaccines
    Wen Yi; Fiscal Year: 2009
    ..In this program, we develop a novel system to produce conjugate vaccines in a facile and widely applicable manner compared to the traditional chemical approach. ..
  21. POPULATION BIOLOGY OF SURFACE POLYSACCHARIDES IN CHOLERA
    John Morris; Fiscal Year: 2004
    ..cholerae surface polysaccharides and, in turn, for the emergence of V. cholerae strains which can form the basis for new cholera pandemics. ..
  22. ACID INDUCIBLE RESPONSES IN H PYLORI
    Catherine McGowan; Fiscal Year: 2003
    ..The proposed work will further elucidate the mechanisms used by H. pylori to colonize and persist within the gastric environment and will lead to improved understanding of how H. pylori causes disease. ..
  23. COMPUTATIONAL ANALYSIS OF CARBOHYDRATE ANTIGENICITY
    Robert Woods; Fiscal Year: 2000
    ..A better understanding of the conformational properties of the serotypes may provide a basis for rational vaccine design. ..
  24. Molecular Pathogenesis of Leptospirosis
    Michael Lovett; Fiscal Year: 2009
    ..The surface antigenic structure and biological properties of HTL and IVCL will be compared. ..
  25. Structure, mechanism and evolution of KDO8P synthase
    DOMENICO GATTI; Fiscal Year: 2007
    ..abstract_text> ..
  26. CAMPYLOBACTER PYLORI IN GASTROINTESTINAL DISEASE
    JOHN PENNER; Fiscal Year: 1990
    ..The longer term objective is to conduct epidemiological studies to trace the sources and routes of transmission of C. pyloridis and obtain convincing evidence to delineate the role of the species in diseases of the stomach and duodenum...
  27. Ecological Fitness of Vibrios and the Emergence of Pathogenic Traits
    Hans Wildschutte; Fiscal Year: 2009
    ..Results may lead to insights on how to monitor and predict bacterial outbreaks. ..
  28. Development of killed vaccine for Vibrio cholerae O139
    Jan Holmgren; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  29. Synthesis of P.Aeruginosa LPS and its Role in Infection
    JOANNA GOLDBERG; Fiscal Year: 2004
    ..aeruginosa strains that have a defective LPS with few or no O antigens; these infections remain localized to the lung. In other P...
  30. Pseudomonas aeruginasa LPS: A Post-Genomic Analysis
    JOANNA GOLDBERG; Fiscal Year: 2004
    ..abstract_text> ..
  31. REGULATION OF O-ANTIGEN SYNTHESIS IN SALMONELLA
    Mary Osborn; Fiscal Year: 1993
    ....
  32. Pseudomonas aeruginosa 1244 Pilin Glycosylation
    Peter Castric; Fiscal Year: 2007
    ..aeruginosa will be established. The membrane topology of this enzyme will also be determined. Finally, the subcellular location of the P. aeruginosa 1244 pilin glycosylation reaction established. [unreadable] [unreadable]..
  33. Studies on the glycosylation of P. aeruginosa pili
    Peter Castric; Fiscal Year: 2002
    ..The project will determine the step in O-antigen biosynthesis that produces the glycan and the subcellular location of pilin glycosylation. ..
  34. ASSAYS FOR ENDOTOXIN IN PLASMA
    Robert Munford; Fiscal Year: 1980
    ....
  35. BACTERIAL MODULATION OF LUNG INFLAMMATORY RESPONSE
    Thomas Russo; Fiscal Year: 2004
    ..g. hemolysin) and bacterially induced host response elements in directly mediating the pathogenesis of lung injury. ..
  36. BRAIN ASSOCIATED HEMATOPOIETIC ANTIGENS
    EDWARD GOLUB; Fiscal Year: 1980
    ..Bone marrow cells are being cultured in the presence of thymic epithelium cells and the expression of brain associated O antigen is being determined...
  37. Resistant E.coli in Humans and Poultry
    James Johnson; Fiscal Year: 2005
    ..Does the research address a current and compelling problem of antimicrobial resistance that is of high public health importance and for which research is needed? [yes]. ..
  38. Adaptive immunity of P. aeruginosa in cystic fibrosis
    GREGORY PRIEBE; Fiscal Year: 2006
    ..Knowledge gained from these studies will elucidate new strategies for the prevention and treatment of P. aeruginosa infections in the setting of CF. [unreadable] [unreadable] [unreadable]..
  39. Cellular immunity to P. aeruginosa candidate vaccines
    GREGORY PRIEBE; Fiscal Year: 2005
    ..As a specialist in pediatric critical care and infectious diseases, his long-term goal is to develop effective immune-based interventions to prevent or ameliorate the consequences of P. aeruginosa infections. ..
  40. Comparative Immunobiology of the bordetellae
    Eric Harvill; Fiscal Year: 2007
    ..Similar mechanisms are likely to be involved in respiratory immunity to other organisms, including those considered potential bioterrorism threats. ..
  41. Biofilm growth and detachment of an oral pathogen
    Jeffrey Kaplan; Fiscal Year: 2007
    ..abstract_text> ..
  42. LIPID A MODIFICATION SYSTEMS IN GRAM-NEGATIVE BACTERIA
    CHRISTIAN RAETZ; Fiscal Year: 2008
    ..leguminosarum lipid A; and IV) i characterization of enzymes that incorporate the unique inner core sugars of/?, leguminosarum LPS. ..
  43. EMERGENCE OF HUMAN PATHOGENS IN THE GENUS BORDETELLA
    Eric Harvill; Fiscal Year: 2007
    ..Finally, these data will parameterize models of the within- and between-host dynamics of these reemerging human pathogens. ..
  44. Center for the Study of Natural Genetic Variation
    Maynard Olson; Fiscal Year: 2005
    ..abstract_text> ..
  45. Genetic clustering and virulence of Y. pestis strains
    Alexander Sulakvelidze; Fiscal Year: 2005
    ..The generated data will also play a critical role in our designing appropriate future studies, and in expanding our Y. pestis research program at the University of Maryland School of Medicine. ..
  46. Male Fertility & Protein Expression in Spermatogenesis
    PAUL RICK; Fiscal Year: 2004
    ..abstract_text> ..
  47. Comparative Genomics of Lyme Disease Spirochetes
    Sherwood Casjens; Fiscal Year: 2004
    ..burgdorferi population. These findings will impact the study of the molecular pathogenesis of Lyme disease in many ways. ..
  48. ANTIGEN DELIVERY SYSTEM DESIGN FOR HOST DEFENSE ANALYSIS
    Roy Curtiss; Fiscal Year: 2004
    ..As improved antigen delivery systems are discovered, it is anticipated that they will construct S. typhi or S. paratyphi A derivatives thereof for eventual clinical studies in humans. ..
  49. Genetics of Survival of Yersinia pseudotuberculosis
    Joan Mecsas; Fiscal Year: 2002
    ..In addition, studies of yop mutant Yersinia strains in mouse strains with specific immune defects may indicate the facets of the immune system targeted by each Yop. ..
  50. FOURTEENTH INTERNATIONAL PATHOGENIC NEISSERIA CONFERENCE
    Michael Apicella; Fiscal Year: 2004
    ..6. To develop new research agenda for the study of the pathogenic Neisseria and to recommend new and future research initiatives and prioritize these. ..
  51. Inhibitors of Yersinia Yop Type III Secretion(RMI)
    Joan Mecsas; Fiscal Year: 2005
    ..In addition, these inhibitors will be valuable tools to probe the structure and function of type III secretion systems encoded by Yersinia species and other Gram-negative bacterial pathogens. ..
  52. Bacteriophage-based probiotic preparations and their effect on the gut microflora
    Alexander Sulakvelidze; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  53. Acquired Immunity and Vaccination for P. aeruginosa
    Gerald Pier; Fiscal Year: 2009
    ..aeruginosa pathogenesis, development of acquired immunity and the potential to develop effective reagents for active and passive immunization to augment the prevention and treatment off, aeruginosa infections. ..
  54. UW Genome Center Large-scale Sequencing Program
    Maynard Olson; Fiscal Year: 2006
    ..Other capabilities of the UWGC such as its automated finishing capacity will be made available to the NHGRI network on an as-needed basis. [unreadable] [unreadable] [unreadable]..
  55. S. typhimiurium Vaccine Against Bacterial Enteropathogens
    Roy Curtiss; Fiscal Year: 2007
    ..abstract_text> ..
  56. Non-replicative Vaccine for Human Brucellosis
    Ramesh Vemulapalli; Fiscal Year: 2011
    ..At present there is no vaccine available for prevention of brucellosis in humans. Research proposed in this application focuses on developing a highly effective and safe Brucella vaccine for human use. ..
  57. Salmonella anti-influenza DNA & antigen delivery vaccine
    Roy Curtiss; Fiscal Year: 2008
    ..abstract_text> ..
  58. DNA packaging and delivery by dsDNA viruses
    Sherwood Casjens; Fiscal Year: 2007
    ..The aims of the project will be pursued through a combination of genetic, biochemical, biophysical and structural analysis strategies. ..
  59. CFTR AND INFECTION OF CYSTIC FIBROSIS PATIENTS
    Gerald Pier; Fiscal Year: 2009
    ..Overall, an enhanced understanding of the normal resistance to P. aeruginosa infection and the role of CFTR in this process will be obtained from the proposed aims. ..
  60. Proteomic studies of pathogenic Leptospira
    Jarlath Nally; Fiscal Year: 2004
    ....