prostaglandin h2

Summary

Summary: A cyclic endoperoxide intermediate produced by the action of CYCLOOXYGENASE on ARACHIDONIC ACID. It is further converted by a series of specific enzymes to the series 2 prostaglandins.

Top Publications

  1. ncbi Cyclooxygenase-2, prostaglandin synthases, and prostaglandin H2 metabolism in traumatic brain injury in the rat
    Tina Kunz
    Department of Pharmaceutical Biosciences, Uppsala Biomedical Center, Uppsala University, Uppsala, Sweden
    J Neurotrauma 19:1051-64. 2002
  2. ncbi Prostaglandin H2 induces the migration of human eosinophils through the chemoattractant receptor homologous molecule of Th2 cells, CRTH2
    Rufina Schuligoi
    Institute of Experimental and Clinical Pharmacology, Medical University of Graz, Universitaetsplatz 4 A 8010 Graz, Austria
    J Leukoc Biol 85:136-45. 2009
  3. ncbi Endothelium-derived prostaglandin H2 evokes the stretch-induced contraction of rabbit pulmonary artery
    Maki Saito
    Department of Pharmacology, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka City, Shizuoka 422 8526, Japan
    Eur J Pharmacol 467:151-61. 2003
  4. ncbi Thromboxane A2/prostaglandin H2-stimulated mitogenesis of coronary artery smooth muscle cells involves activation of mitogen-activated protein kinase and S6 kinase
    T A Morinelli
    Department of Medicine, Medical University of South Carolina, Charleston 29425
    J Biol Chem 269:5693-8. 1994
  5. ncbi Characterization of the lysyl adducts formed from prostaglandin H2 via the levuglandin pathway
    O Boutaud
    Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232 6602, USA
    Biochemistry 38:9389-96. 1999
  6. pmc Structural basis for induced formation of the inflammatory mediator prostaglandin E2
    Caroline Jegerschöld
    Department of Biosciences and Nutrition, Karolinska Institutet and School of Technology and Health, Royal Institute of Technology, Novum, S 141 57 Huddinge, Sweden
    Proc Natl Acad Sci U S A 105:11110-5. 2008
  7. ncbi Cloning and crystal structure of hematopoietic prostaglandin D synthase
    Y Kanaoka
    Department of Molecular Behavioral Biology, Osaka Bioscience Institute, Japan
    Cell 90:1085-95. 1997
  8. ncbi Biochemical and immunological demonstration of prostaglandin D2, E2, and F2 alpha formation from prostaglandin H2 by various rat glutathione S-transferase isozymes
    M Ujihara
    Department of Dermatology, Kyoto University Faculty of Medicine, Japan
    Arch Biochem Biophys 264:428-37. 1988
  9. ncbi Role of prostaglandin H2 as an endothelium-derived contracting factor in diabetic state
    K Shimizu
    Department of Internal Medicine II, Nagoya University School of Medicine, Japan
    Diabetes 42:1246-52. 1993
  10. ncbi Prostaglandin F2alpha formation from prostaglandin H2 by prostaglandin F synthase (PGFS): crystal structure of PGFS containing bimatoprost
    Junichi Komoto
    Department of Molecular Biosciences, University of Kansas, 1200 Sunnyside Avenue, Lawrence, Kansas 66045 7534, USA
    Biochemistry 45:1987-96. 2006

Detail Information

Publications201 found, 100 shown here

  1. ncbi Cyclooxygenase-2, prostaglandin synthases, and prostaglandin H2 metabolism in traumatic brain injury in the rat
    Tina Kunz
    Department of Pharmaceutical Biosciences, Uppsala Biomedical Center, Uppsala University, Uppsala, Sweden
    J Neurotrauma 19:1051-64. 2002
    ....
  2. ncbi Prostaglandin H2 induces the migration of human eosinophils through the chemoattractant receptor homologous molecule of Th2 cells, CRTH2
    Rufina Schuligoi
    Institute of Experimental and Clinical Pharmacology, Medical University of Graz, Universitaetsplatz 4 A 8010 Graz, Austria
    J Leukoc Biol 85:136-45. 2009
    ..These data indicate that PGH2 causes activation of the PGD2 receptors CRTH2 and DP via a dual mechanism: by interacting directly with the receptors and/or by giving rise to PGD2 after catalytic conversion by plasma proteins...
  3. ncbi Endothelium-derived prostaglandin H2 evokes the stretch-induced contraction of rabbit pulmonary artery
    Maki Saito
    Department of Pharmacology, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka City, Shizuoka 422 8526, Japan
    Eur J Pharmacol 467:151-61. 2003
    ..The results suggested that prostaglandin H(2) and thromboxane A(2), either released from endothelium by mechanical stretch or by acetylcholine, produced contraction of rabbit pulmonary artery in a RGD-independent manner...
  4. ncbi Thromboxane A2/prostaglandin H2-stimulated mitogenesis of coronary artery smooth muscle cells involves activation of mitogen-activated protein kinase and S6 kinase
    T A Morinelli
    Department of Medicine, Medical University of South Carolina, Charleston 29425
    J Biol Chem 269:5693-8. 1994
    b>Prostaglandin H2 (PGH2) and thromboxane A2 (TXA2) are potent activators of platelets and vascular smooth muscle whose responses are mediated through a common G-protein coupled receptor (TXA2/PGH2 receptor)...
  5. ncbi Characterization of the lysyl adducts formed from prostaglandin H2 via the levuglandin pathway
    O Boutaud
    Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232 6602, USA
    Biochemistry 38:9389-96. 1999
    ..These findings provide a basis for investigating protein modification induced by oxygenation of arachidonic acid by the cyclooxygenases...
  6. pmc Structural basis for induced formation of the inflammatory mediator prostaglandin E2
    Caroline Jegerschöld
    Department of Biosciences and Nutrition, Karolinska Institutet and School of Technology and Health, Royal Institute of Technology, Novum, S 141 57 Huddinge, Sweden
    Proc Natl Acad Sci U S A 105:11110-5. 2008
    ..Hence, insight into the dynamic behavior of MPGES1 and homologous membrane proteins in inflammation and detoxification is provided...
  7. ncbi Cloning and crystal structure of hematopoietic prostaglandin D synthase
    Y Kanaoka
    Department of Molecular Behavioral Biology, Osaka Bioscience Institute, Japan
    Cell 90:1085-95. 1997
    ..The unique 3-D architecture of the cleft leads to the putative substrate binding mode and its catalytic mechanism, responsible for the specific isomerization from PGH2 to PGD2...
  8. ncbi Biochemical and immunological demonstration of prostaglandin D2, E2, and F2 alpha formation from prostaglandin H2 by various rat glutathione S-transferase isozymes
    M Ujihara
    Department of Dermatology, Kyoto University Faculty of Medicine, Japan
    Arch Biochem Biophys 264:428-37. 1988
    ..2-2, 3-3, 3-4, and 4-4), liver with hyperplastic nodules (7-7), brain (Yn1Yn1), and testis (Yn1Yn2) all had prostaglandin H2-converting activity...
  9. ncbi Role of prostaglandin H2 as an endothelium-derived contracting factor in diabetic state
    K Shimizu
    Department of Internal Medicine II, Nagoya University School of Medicine, Japan
    Diabetes 42:1246-52. 1993
    This study investigated the possible involvement of prostaglandin H2, an acetylcholine-induced endothelium-derived contracting factor in rat aorta, in the development of abnormality of the vasculature in diabetes...
  10. ncbi Prostaglandin F2alpha formation from prostaglandin H2 by prostaglandin F synthase (PGFS): crystal structure of PGFS containing bimatoprost
    Junichi Komoto
    Department of Molecular Biosciences, University of Kansas, 1200 Sunnyside Avenue, Lawrence, Kansas 66045 7534, USA
    Biochemistry 45:1987-96. 2006
    ..Formation of PGF(2)(alpha) from PGH(2) most likely involves a direct hydride transfer from the bound NADPH to the endoperoxide of PGH(2) without the participation of specific amino acid residues...
  11. ncbi Isolation and characterization of an enzyme from sheep seminal vesicles that catalyzes the glutathione-dependent reduction of prostaglandin H2 to prostaglandin F2 alpha
    J R Burgess
    Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907, USA
    Biochem Mol Biol Int 41:217-26. 1997
    An enzyme which catalyzes the direct 2-electron reduction of prostaglandin H2 to prostaglandin F2 alpha has been purified from the microsomes of sheep seminal vesicles...
  12. ncbi Prostaglandin H2 (PGH2) accelerates formation of amyloid beta1-42 oligomers
    Olivier Boutaud
    Department of Pharmacology, Center for Molecular Neuroscience, Vanderbilt University, Nashville, Tennessee, USA
    J Neurochem 82:1003-6. 2002
    ..The product of the oxygenation of arachidonic acid by the cyclooxygenases, prostaglandin H2 (PGH2), rearranges non-enzymatically to several prostaglandins, including the highly reactive gamma-keto ..
  13. ncbi Amino acid substitutions in the human glutathione S-transferases confer different specificities in the prostaglandin endoperoxide conversion pathway
    J R Burgess
    Department of Veterinary Science, Pennsylvania State University, University Park 16802
    Biochem Biophys Res Commun 158:497-502. 1989
    ..Using prostaglandin H2 as the peroxide substrate, we found that GSH S-transferase 1-1 catalyzed the transformation of prostaglandin ..
  14. ncbi Structural and functional characterization of human microsomal prostaglandin E synthase-1 by computational modeling and site-directed mutagenesis
    Xiaoqin Huang
    Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 725 Rose Street, Lexington, KY 40536, USA
    Bioorg Med Chem 14:3553-62. 2006
    ..three-dimensional (3D) model of the substrate-binding domain (SBD) of mPGES-1 and its binding with substrates prostaglandin H2 (PGH2) and glutathione (GSH)...
  15. ncbi PGH2 degradation pathway catalyzed by GSH-heme complex bound microsomal prostaglandin E2 synthase type 2: the first example of a dual-function enzyme
    Taro Yamada
    Department of Molecular Biosciences, University of Kansas, 1200 Sunnyside Avenue, Lawrence, Kansas 66045 7534, USA
    Biochemistry 46:8414-24. 2007
    ..Since mPGES-2 is an isomerase and mPGES-2h is a lyase, mPGES-2 cannot simply be classified into one of six classes set by the International Union of Biochemistry and Molecular Biology...
  16. ncbi Prostaglandin H2 as an endothelium-derived contracting factor and its interaction with endothelium-derived nitric oxide
    T Ito
    2nd Department of Internal Medicine, Nagoya University School of Medicine, Japan
    J Hypertens 9:729-36. 1991
    The possibility that prostaglandin H2 is an endothelium-derived contracting factor (EDCF) was evaluated in rings of thoracic aorta of spontaneously hypertensive rats (SHR)...
  17. ncbi Mesangial cells release untransformed prostaglandin H2 as a major prostanoid
    M Soler
    Laboratori de Mediadors de la Inflamació, Institut de Recerca de Hospital de la Santa Creu i Sant Pau, Fundacio Puigvert, Barcelona, Spain
    Kidney Int 59:1283-9. 2001
    b>Prostaglandin H2 (PGH2) is the precursor of the other prostanoids and exhibits a vasoconstricting activity. Glomerular mesangial cells are an important source of vasoactive prostanoids in kidney...
  18. ncbi Endothelium-dependent contractions are associated with both augmented expression of prostaglandin H synthase-1 and hypersensitivity to prostaglandin H2 in the SHR aorta
    T Ge
    Center for Experimental Therapeutics, Baylor College of Medicine, Houston, TX 77030, USA
    Circ Res 76:1003-10. 1995
    b>Prostaglandin H2 (PGH2 [endoperoxide]) is an immediate product of prostaglandin H (PGH) synthase activity (cyclooxygenase) and a likely candidate to mediate endothelium-dependent contractions evoked by acetylcholine in the aorta of the ..
  19. ncbi Prostaglandin H2 as an endothelium-derived contracting factor modulates endothelin-1-induced contraction
    H Asano
    Department of Internal Medicine II, Nagoya University School of Medicine, Japan
    J Hypertens 12:383-90. 1994
    To investigate the possible involvement of prostaglandin H2, an endothelium-derived contracting factor in the rat aorta, in the development of the contraction induced by endothelin-1.
  20. ncbi Cooperative mediation by serotonin S2 and thromboxane A2/prostaglandin H2 receptor activation of cyclic flow variations in dogs with severe coronary artery stenoses
    J H Ashton
    Department of Internal Medicine Cardiology Division, University of Texas Health Science Center at Dallas 75235 9047
    Circulation 76:952-9. 1987
    ..Thus, the data obtained in these studies demonstrate a cooperative interaction between thromboxane A2/PGH2 and serotonin S2 receptors that contributes to the development of CFVs in this experimental preparation...
  21. ncbi Prostaglandin H2 may be the endothelium-derived contracting factor released by acetylcholine in the aorta of the rat
    T Kato
    2nd Department of Internal Medicine, Nagoya University School of Medicine, Japan
    Hypertension 15:475-81. 1990
    ..were significantly enhanced by pretreatment with a cyclooxygenase inhibitor (indomethacin) or thromboxane A2/prostaglandin H2 receptor antagonist (ONO-3708) in aortic rings from both SHR and WKY rats...
  22. ncbi Thromboxane synthase: structure and function of protein and gene
    Lee Ho Wang
    Department of Internal Medicine, University of Texas Health Science Center at Houston, 77030, USA
    Prostaglandins Other Lipid Mediat 68:409-22. 2002
  23. ncbi Prostaglandin H2 and thromboxane A2 are contractile factors in intrarenal arteries of spontaneously hypertensive rats
    F X Dai
    Department of Medicine, University of Kansas Medical Center, Kansas City 66103
    Hypertension 19:795-8. 1992
    ..Inhibition of cyclooxygenase (indomethacin) or blockade of prostaglandin H2-thromboxane A2 receptors (SQ 29,548) blocked acetylcholine-induced contractions in SHR renal arteries; ..
  24. ncbi Expression of prostaglandin H2-mediated mechanism of vascular contraction in hypertensive rats. Relation to lipoxygenase and prostacyclin synthase activities
    L Lin
    Department of Pharmacology, New York Medical College, Valhalla 10595
    Circ Res 74:197-205. 1994
    ..abstract truncated at 250 words)..
  25. ncbi Endothelin and prostaglandin H2 enhance arteriolar myogenic tone in hypertension
    A Huang
    Department of Physiology, New York Medical College, Valhalla, 10595, USA
    Hypertension 30:1210-5. 1997
    ..Thus, in SH rats the enhanced arteriolar constriction to increases in intravascular pressure seems to be due to the production of endothelium-derived constrictor factors PGH2 and endothelin...
  26. pmc Mutation of a critical arginine in microsomal prostaglandin E synthase-1 shifts the isomerase activity to a reductase activity that converts prostaglandin H2 into prostaglandin F2alpha
    Tove Hammarberg
    Department of Medical Biochemistry and Biophysics, Division of Chemistry II, Karolinska Institutet, S 171 77 Stockholm, Sweden
    J Biol Chem 284:301-5. 2009
    ..and R126Q mPGES-1 exhibit a novel, glutathione-dependent, reductase activity, which allows conversion of prostaglandin H2 into prostaglandin F2alpha...
  27. ncbi Evidence for a bidirectional prostaglandin endoperoxide shunt between platelets and the bovine coronary artery
    P R Mayeux
    Department of Pharmacology, Tulane University School of Medicine, New Orleans, LA 70112
    Biochim Biophys Acta 1011:18-24. 1989
    ..These data also suggest a potential for a bidirectional exchange of PGH2 between platelets and vascular wall during platelet-vascular wall interactions...
  28. ncbi Mass spectrometric analysis of leukotriene A4 and other chemically reactive metabolites of arachidonic acid
    Jennifer S Dickinson
    Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
    J Am Soc Mass Spectrom 13:1227-34. 2002
    ..leukotrienes proceeds through the formation of chemically reactive intermediates leukotriene A4 (LTA4) and prostaglandin H2 (PGH2) which in aqueous solutions have chemical half-lives of 3 s and 3 min, respectively...
  29. ncbi Kinetics of radical-scavenging activity of hesperetin and hesperidin and their inhibitory activity on COX-2 expression
    Atsusi Hirata
    Department of Oral Diagnosis, Meikai University School of Dentistry, 350 0283 Saitama, Japan
    Anticancer Res 25:3367-74. 2005
    ..7 cells, suggesting the anti-inflammatory activity of these compounds. The ability of hesperetin and hesperidin to suppress COX-2 gene expression may be a consequence of their antioxidant activity...
  30. ncbi Bovine prostacyclin synthase: purification and isolation of partial cDNA
    B Pereira
    Department of Internal Medicine, University of Texas Houston 77030
    Biochem Biophys Res Commun 197:1041-8. 1993
    ..Amino acid sequences of N-terminus and a tryptic peptide led us to isolate a partial cDNA fragment...
  31. ncbi Prostaglandin endoperoxides and thromboxane A2 activate the same receptor isoforms in human platelets
    Roberta Vezza
    Center for Experimental Therapeutics, University of Pennsylvania, Philadelphia, USA
    Thromb Haemost 87:114-21. 2002
    ..These data show that endoperoxides and TXA2 mediate their effects on platelets acting on both, and the same, receptor isoform(s)...
  32. ncbi [Prostaglandin E2 synthases]
    Yoshihito Nakatani
    Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, 1 5 8 Hatanodai, Shinagawa ku, Tokyo 142 8555, Japan
    Nihon Yakurigaku Zasshi 120:373-8. 2002
    ..Most recently, glutathione-nonspecific mPGES-2, homologous to glutaredoxin and thioredoxin, was identified. These PGESs seem to be a potential novel target for drug development...
  33. ncbi Purification and characterization of recombinant human prostacyclin synthase
    Masayuki Wada
    Department of Pharmacology, National Cardiovascular Center Research Institute and Division of Microcirculatory Kinetics, Osaka University Graduate School of Medicine, National Cardiovascular Center, 5 7 1 Fujishiro dai, Suita, Osaka 565 8565
    J Biochem 135:455-63. 2004
    ....
  34. ncbi New developments on thromboxane and prostacyclin modulators part I: thromboxane modulators
    Jean Michel Dogne
    Natural and Synthetic Drug Research Center, Medicinal Chemistry, University of Liege, 1, Av de l Hôpital, B 4000 Liege, Belgium
    Curr Med Chem 11:1223-41. 2004
    ..In this review, we will propose a description of the recently described thromboxane modulators of major interest from both a pharmacological and a chemical point of view...
  35. ncbi Mechanisms of enhanced macrophage-mediated prostaglandin E2 production and its suppressive role in Th1 activation in Th2-dominant BALB/c mice
    Etsushi Kuroda
    Department of Immunology, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan
    J Immunol 170:757-64. 2003
    ....
  36. ncbi Characterization of the substrate mimic bound to engineered prostacyclin synthase in solution using high-resolution NMR spectroscopy and mutagenesis: implication of the molecular mechanism in biosynthesis of prostacyclin
    Ke He Ruan
    The Center for Experimental Therapeutics and PharmacoInformatics and Department of Pharmacological and Pharmaceutical Sciences, College of Pharmacy, University of Houston, Houston, Texas 77204, USA
    Biochemistry 47:680-8. 2008
    High-resolution NMR spectroscopy was used to determine the docking of a substrate (prostaglandin H2) mimic (U46619) to the engineered prostacyclin (PGI2) synthase (PGIS) in solution...
  37. ncbi Structure of the extracellular glutathione S-transferase OvGST1 from the human pathogenic parasite Onchocerca volvulus
    Markus Perbandt
    Institute of Biochemistry, Center for Structural and Cell Biology, University of Luebeck, Ratzeburger Allee 160, 23538 Luebeck, Germany
    J Mol Biol 377:501-11. 2008
    ..Finally, a putative substrate binding mode for prostaglandin H(2) is postulated based on the observed structural insights...
  38. ncbi Antiplatelet effect of NQ12: a possible mechanism through the arachidonic acid cascade
    Yong Ri Jin
    Research Institute of Veterinary Medicine, CBITRC, Chungbuk National University, Cheongju, Korea
    J Pharmacol Sci 105:193-200. 2007
    ..This may be a convincing mechanism for the antithrombotic action of NQ12...
  39. ncbi Increased myogenic responsiveness of skeletal muscle arterioles with juvenile growth
    Julie Balch Samora
    Center for Interdisciplinary Research in Cardiovascular Sciences, Robert C Byrd Health Sciences Center, West Virginia University School of Medicine, Morgantown, WV 26505 9105, USA
    Am J Physiol Heart Circ Physiol 294:H2344-51. 2008
    ..As a result, the relative contribution of myogenic activity to blood flow regulation in skeletal muscle may increase during rapid juvenile growth...
  40. ncbi Understanding microscopic binding of human microsomal prostaglandin E synthase-1 with substrates and inhibitors by molecular modeling and dynamics simulation
    Adel Hamza
    Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 725 Rose Street, Lexington, Kentucky 40536, USA
    J Phys Chem B 112:7320-9. 2008
    ..The new structural insights obtained from this study should be valuable for rational design of next-generation anti-inflammatory drugs...
  41. ncbi Eicosanoid transcellular biosynthesis: from cell-cell interactions to in vivo tissue responses
    Giancarlo Folco
    Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Mail Stop 8303, 12801 E 17th Avenue, P O Box 6511, Aurora, CO 80045 0511, USA
    Pharmacol Rev 58:375-88. 2006
    ..Experiments for transcellular events taking place in vivo that reveal the true complexity of eicosanoid biosynthesis within tissues are also reviewed...
  42. ncbi Involvement of protein kinase C and Na+/K+-ATPase in the contractile response induced by myricetin in rat isolated aorta
    Rosario Jimenez
    Department of Pharmacology, School of Pharmacy, University of Granada, Granada, Spain
    Planta Med 68:133-7. 2002
    ..These effects appear to be related to the activation of PGH2-TXA2 receptors on vascular smooth muscle by the TXA2 released from endothelium...
  43. ncbi Inhibition of inducible prostaglandin E(2) synthase by 15-deoxy-Delta(12,14)-prostaglandin J(2) and polyunsaturated fatty acids
    Omar Quraishi
    Department of Biochemistry and Molecular Biology, Merck Frosst Centre for Therapeutic Research, Kirkland, Que, Canada
    Biochem Pharmacol 63:1183-9. 2002
    ....
  44. ncbi Lipopolysaccharide evokes microaggregation reactions in hemocytes isolated from tobacco hornworms, Manduca sexta
    Jon S Miller
    Department of Biological Sciences, Northern Illinois University, De Kalb, IL 60115, USA
    Comp Biochem Physiol A Mol Integr Physiol 137:285-95. 2004
    ..We infer that isolated hemocyte preparations recognize and react to LPS by forming microaggregates and this reaction is mediated by PGs, but not products of the LOX pathway...
  45. ncbi Cloning and characterization of CYP4F21: a prostaglandin E2 20-hydroxylase of ram seminal vesicles
    J Bylund
    Department of Pharmaceutical Biosciences, Uppsala Biomedical Center, Uppsala University, Sweden
    Arch Biochem Biophys 389:123-9. 2001
    ..The reproductive function of CYP4F21 is likely to biosynthesize 20-hydroxy-PGE1 and 20-hydroxy-PGE2, which is excreted by the seminal vesicles...
  46. ncbi Substrate binding is the rate-limiting step in thromboxane synthase catalysis
    L h Wang
    Division of Hematology, Department of Internal Medicine, University of Texas, Houston, Texas 77030, USA
    J Biol Chem 276:14737-43. 2001
    ..Without any need for an external electron donor, or for a reductase or molecular oxygen, it uses prostaglandin H2 (PGH2) to catalyze either an isomerization reaction to form thromboxane A2 (TXA2) or a fragmentation reaction ..
  47. pmc Identification of a novel prostaglandin f(2alpha) synthase in Trypanosoma brucei
    B K Kubata
    Department of Molecular Behavioral Biology, Osaka Bioscience Institute, Osaka 565 0874, Japan
    J Exp Med 192:1327-38. 2000
    ..The characterization of TbPGFS and its gene in T. brucei provides a basis for the molecular analysis of the role of parasite-derived PGF(2alpha) in the physiology of the parasite and the pathogenesis of African trypanosomiasis...
  48. ncbi Structural insights into the stereochemistry of the cyclooxygenase reaction
    J R Kiefer
    Searle Discovery Research, Monsanto Company, St Louis, Missouri 63198, USA
    Nature 405:97-101. 2000
    ..The subsequent peroxidase reaction reduces prostaglandin G2 to prostaglandin H2. Here we report the co-crystal structures of murine apo-cyclooxygenase-2 in complex with arachidonic acid and ..
  49. ncbi [Sleep and brain function]
    Yoshihiro Urade
    Department of Molecular Behavioral Biology, Osaka Bioscience Institute, Suita
    No To Hattatsu 38:331-3. 2006
    ..The GABAergic inhibition of TMN is involved in non-REM sleep induction by PGD2 or adenosine A2A receptor-agonists. The neural network between VLPO and TMN is considered to play a key role in the regulation of vigilance states...
  50. ncbi HTRF-based assay for microsomal prostaglandin E2 synthase-1 activity
    Eric R Goedken
    Abbott Bioresearch Center, Worcester, Massachusetts 01605, USA
    J Biomol Screen 13:619-25. 2008
    ..E2 synthase-1 (mPGES-1) catalyzes the formation of prostaglandin E2 (PGE2) from the endoperoxide prostaglandin H2 (PGH2)...
  51. ncbi Oxidation of prostaglandin H(2) and prostaglandin H(2) analogues by human cytochromes P450: analysis of omega-side chain hydroxy metabolites and four steroisomers of 5-hydroxyprostaglandin I(1) by mass spectrometry
    E H Oliw
    Division of Biochemical Pharmacology, Department of Pharmaceutical Biosciences, Uppsala Biomedical Centre, Uppsala University, SE 751 24 Uppsala, Sweden
    Biochem Pharmacol 62:407-15. 2001
    ..We conclude that omega-side chain hydroxylation of PGH(2) analogues may be catalyzed by many different cytochromes P450, but only CYP4F8 oxidizes the omega-side chain of PGH(2) efficiently...
  52. ncbi Levuglandins and isolevuglandins: stealthy toxins of oxidative injury
    Robert G Salomon
    Department of Chemistry, Case Western Reserve University, Cleveland, OH 44106 7078, USA
    Antioxid Redox Signal 7:185-201. 2005
    ..Because they can accumulate over the lifetimes of proteins, iso[n]LG-protein adducts represent a convenient dosimeter of oxidative stress...
  53. ncbi Solution structure of a common substrate mimetic of cyclooxygenase-downstream synthases bound to an engineered thromboxane A2 synthase using a high-resolution NMR technique
    Ke He Ruan
    Vascular Biology Research Center, Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, Houston, 77030, USA
    Arch Biochem Biophys 444:165-73. 2005
    ..The NMR technique can be used as a powerful tool to determine the conformations of PGH(2) bound to other COX-downstream synthases...
  54. ncbi Characterization of bis(levuglandinyl) urea derivatives as products of the reaction between prostaglandin H2 and arginine
    Irene Zagol-Ikapitte
    Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232 6602, USA
    Biochemistry 43:5503-10. 2004
    ..The soluble characteristic of these molecules provides a potential strategy for development of biological markers of lipid modification of proteins following cyclooxygenase activity or lipid peroxidation...
  55. ncbi Prostaglandin H(2)-derived adducts of proteins correlate with Alzheimer's disease severity
    Irene Zagol-Ikapitte
    Department of Pharmacology, Vanderbilt University, Nashville, TN, USA
    J Neurochem 94:1140-5. 2005
    ..The enzymatic product of the cyclooxygenases, prostaglandin H2, rearranges in part to highly reactive gamma-ketoaldehydes, levuglandin (LG) E(2) and LGD(2)...
  56. pmc PGH2-derived levuglandin adducts increase the neurotoxicity of amyloid beta1-42
    Olivier Boutaud
    Department of Pharmacology, Vanderbilt University, Nashville 37232 6602, Tennessee, USA
    J Neurochem 96:917-23. 2006
    ....
  57. ncbi Structural and mutational analysis of Trypanosoma brucei prostaglandin H2 reductase provides insight into the catalytic mechanism of aldo-ketoreductases
    Kubata Bruno Kilunga
    Department of Molecular Behavioral Biology, Osaka Bioscience Institute, 6 2 4 Furuedai, Suita, Osaka 565 0874, Japan
    J Biol Chem 280:26371-82. 2005
    Trypanosoma brucei prostaglandin F2alpha synthase is an aldo-ketoreductase that catalyzes the reduction of prostaglandin H2 to PGF2alpha in addition to that of 9,10-phenanthrenequinone. We report the crystal structure of TbPGFS.NADP+...
  58. ncbi Vascular dysfunction in type 2 diabetic TallyHo mice: role for an increase in the contribution of PGH2/TxA2 receptor activation and cytochrome p450 products
    Zhong Jian Cheng
    Smooth Muscle Research Group, Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Calgary, AB T2N 4N1, Canada
    Can J Physiol Pharmacol 85:404-12. 2007
    ..The present study indicates that enhanced contribution of prostaglandin H2/thromboxane A2 receptor and CYP, likely CYP2C6 and 2C9, play a critical role in the pathogenesis of increased ..
  59. ncbi Effects of aerosol vs IV UT-15 on prostaglandin H2 analog-induced pulmonary hypertension in sheep
    Brett L Sandifer
    Center for Translational Research in the Lung and the Andrew J McKelvey Lung Transplantation Center, Division of Pulmonary, Allergy and Critical Care Medicine, Emory University, Atlanta, GA, USA
    Chest 128:616S. 2005
  60. pmc Induction by endothelin-1 of epithelium-dependent relaxation of guinea-pig trachea in vitro: role for nitric oxide
    J G Filep
    Department of Pharmacology, Faculty of Medicine, University of Sherbrooke, Canada
    Br J Pharmacol 109:637-44. 1993
    ..Preincubation of the tissues with the thromboxane A2/prostaglandin H2 receptor antagonist, BM 13505 (5 microM) significantly potentiated the relaxant response to ET-1. 3...
  61. pmc Effect of cytoplasmic pH on Ca(2+)-stimulated eicosanoid biosynthesis in human platelets
    J S Edwards
    Department of Clinical Pharmacology, United Medical School, Guy s Hospital, London
    Br J Pharmacol 113:926-30. 1994
    ..5 +/- 26.7 to 203.2 +/- 36.4 ng microg-1 protein. Responses of [Ca2+]i and TXB2 production to epoxymethano prostaglandin H2 (U46619, an endoperoxide-thromboxane receptor agonist) increased significantly in the presence of NH4C1.4...
  62. ncbi Binding of a thromboxane A2 (TXA2)/prostaglandin H2 (PGH2) receptor antagonist to rabbit and pig heart membrane protein
    B Lasserre
    , Institut de Physiologie, Toulouse, France
    Prostaglandins Leukot Essent Fatty Acids 47:153-7. 1992
    ....
  63. ncbi [Mediators of the vascular endothelium as markers of myocardial ischemia and reperfusion]
    D Jambou
    Service de cardiologie, , Nice
    Arch Mal Coeur Vaiss 86:57-62. 1993
    ....
  64. pmc Induction of cyclo-oxygenase-2 by cytokines in human pulmonary epithelial cells: regulation by dexamethasone
    J A Mitchell
    Department of Applied Pharmacology, National Heart and Lung Institute, London
    Br J Pharmacol 113:1008-14. 1994
    1. Cyclo-oxygenase metabolizes arachidonic acid to prostaglandin H2 (PGH2) and exists in at least two isoforms...
  65. ncbi Significance of endothelial prostacyclin and nitric oxide in peripheral and pulmonary circulation
    R J Gryglewski
    Jagiellonian University, 16 Grzegorzecka str, 31 531 Cracow, Poland
    Med Sci Monit 7:1-16. 2001
    ..We looked for mechanisms of protective endothelial function, with a special respect to the differences between peripheral and pulmonary circulation...
  66. ncbi Dihydropyridine agonist Bay K 8644 inhibits platelet activation by competitive antagonism of thromboxane A2-prostaglandin H2 receptor
    G J Johnson
    Department of Medicine, Veterans Administration Medical Center, Minneapolis, Minnesota 55417
    Circ Res 62:494-505. 1988
    ..Since these results suggested inhibition of the TXA2-prostaglandin H2 (PGH2) receptor, we measured binding of [3H]U46619 to intact platelets...
  67. ncbi Mechanism of the contractile effect of diaspirin-cross-linked hemoglobin in rat isolated aorta strip denuded of endothelium as revealed using an oil-immersion procedure
    F Rioux
    Centre de Recherche, , , Canada
    Can J Physiol Pharmacol 74:1171-9. 1996
    ..It was abolished by indomethacin, SQ-29548 (prostaglandin H2-thromboxane A2 receptor antagonist), thiourea, or N-2-mercaptopropionylglycine (MCPG), reduced partially by ..
  68. ncbi Thromboxane A2 and peptidoleukotrienes contribute to the myocardial ischemia and contractile dysfunction in response to intracoronary infusion of complement C5a in pigs
    B R Ito
    Department of Medicine, Veterans Administration Medical Center, San Diego, CA 92161
    Circ Res 66:596-607. 1990
    ..v.) (n = 5), 2) thromboxane A2/prostaglandin H2 receptor blockade with thromboxane receptor blocker BM13505 (2 mg/kg i.v...
  69. ncbi Selective modulation of the human platelet thromboxane A2/prostaglandin H2 receptor by eicosapentaenoic and docosahexaenoic acids in intact platelets and solubilized platelet membranes
    C A Parent
    Department of Pharmacology, University of Illinois, Chicago 60612
    J Biol Chem 267:6541-7. 1992
    ..of these fatty acids resulted in a decrease in the affinity of [3H]U46619 for the thromboxane A2/prostaglandin H2 (TXA2/PGH2) receptor...
  70. ncbi Role of prostaglandins in acetylcholine-induced contraction of aorta from spontaneously hypertensive and Wistar-Kyoto rats
    R M Rapoport
    Department of Pharmacology, Veterans Affairs Medical Center, University of Cincinnati, Ohio, College of Medicine 45267 0575, USA
    Hypertension 28:64-75. 1996
    ..Thus, we propose that PGI2 released in response to acetylcholine may serve as the endothelium-derived contracting factor that elicits the TxA2/PGH2 receptor-independent and dependent components of the acetylcholine-induced contraction...
  71. pmc Endogenous prostaglandin endoperoxides and prostacyclin modulate the thrombolytic activity of tissue plasminogen activator. Effects of simultaneous inhibition of thromboxane A2 synthase and blockade of thromboxane A2/prostaglandin H2 receptors in a canine
    P Golino
    Texas Heart Institute, University of Texas Health Science Center, Houston 77225
    J Clin Invest 86:1095-102. 1990
    ....
  72. pmc Heterogeneity of thromboxane A2 (TP-) receptors: evidence from antagonist but not agonist potency measurements
    P M Tymkewycz
    Department of Pharmacology, University of Edinburgh
    Br J Pharmacol 102:607-14. 1991
    ..However, classification into TP,-, TP2-receptors, etc. on the basis of the limited antagonist data available does not appear appropriate at this time...
  73. ncbi Bradykinin as a major endogenous regulator of endothelial function
    Ryszard J Gryglewski
    Jagiellonian University, Cracow, Poland
    Pediatr Pathol Mol Med 21:279-90. 2002
    ..Activation of HO-1 may lead to a deficiency in intracellular heme required as a cofactor for both COX and NOS. This network of interactions triggered by Bk call for further studies...
  74. ncbi Polyunsaturated fatty acids (PUFA) and eicosanoids in human health and pathologies
    H Tapiero
    UNIVERSITE DE PARIS, Faculté de Pharmacie CNRS UMR 8612, Chatenay Malabry, France
    Biomed Pharmacother 56:215-22. 2002
    ....
  75. ncbi Interactions of nonprostanoid trimetoquinol analogs with thromboxane A2/prostaglandin H2 receptors in human platelets, rat vascular endothelial cells and rat vascular smooth muscle cells
    Y Shin
    Division of Pharmacology, College of Pharmacy, Ohio State University, Columbus 43210 1291
    J Pharmacol Exp Ther 267:1017-23. 1993
    ..TMQ analogs were used to examine the stereochemical requirements of the interaction with thromboxane A2/prostaglandin H2 (TP) receptor sites in human platelets (HP), and cultured rat vascular endothelial (RVEC) and smooth muscle (..
  76. ncbi The influence of isoprostanes on ADP-induced platelet aggregation and cyclic AMP-generation in human platelets
    N Leitinger
    Department of Nuclear Medicine, University of Vienna, Austria
    Thromb Res 86:337-42. 1997
    ..In this study we investigate possible pro- or antiaggregatory properties of various F- and E-type isoprostanes on human platelets...
  77. ncbi Studies on the antithrombotic action of AT1 receptor antagonists
    W Buczko
    Department of Pharmacodynamics, Medical University of Bialystok, Poland
    Med Sci Monit 7:600-5. 2001
    ..that apart from blocking AT1 receptor, losartan is also a competitive antagonist to thromboxane A2/prostaglandin H2 receptor (TP receptor)...
  78. ncbi Estrogen replacement suppresses a prostaglandin H synthase-dependent vasoconstrictor in rat mesenteric arteries
    S T Davidge
    Perinatal Research Centre, Department of Ob Gyn, University of Alberta, Edmonton, Canada
    Circ Res 83:388-95. 1998
    ..In the presence of PGHS inhibitors (meclofenamate, valeryl salicylate, and NS-398) or a thromboxane A2 (TxA2)/prostaglandin H2 (PGH2) receptor blocker (SQ-29548), there was no longer a significant difference among the groups...
  79. pmc Potentiation by endothelin-1 of 5-hydroxytryptamine responses in aortae from streptozotocin-diabetic rats: a role for thromboxane A2
    G M James
    Department of Pharmacology, Monash University, Clayton, Victoria, Australia
    Br J Pharmacol 114:1236-40. 1995
    ..2. In the present study, the thromboxane A2/prostaglandin H2 (TP) receptor antagonist GR32191B (1 microM) significantly reduced maximum responses to 5-HT in endothelium-..
  80. ncbi Effect of specific phospholipid molecular species incorporated in human platelet membranes on thromboxane A2/prostaglandin H2 receptors
    Y Bayon
    , Laboratoire de Chimie Biologique INSA Lyon, Villeurbanne, France
    J Lipid Res 36:47-56. 1995
    ..3H]U46619 specific binding to thromboxane A2/prostaglandin H2 (TXA2/PGH2) receptors, as well as specific binding of the antagonist [3H]SQ29548 to these sites were also ..
  81. ncbi Endogenous prostaglandin endoperoxides may alter infarct size in the presence of thromboxane synthase inhibition: studies in a rabbit model of coronary artery occlusion-reperfusion
    P Golino
    Department of Internal Medicine, University of Naples, Italy
    J Am Coll Cardiol 21:493-501. 1993
    ....
  82. pmc EP 171: a high affinity thromboxane A2-mimetic, the actions of which are slowly reversed by receptor blockade
    R L Jones
    Department of Pharmacology, University of Edinburgh
    Br J Pharmacol 96:875-87. 1989
    1. Replacement of the four-carbon omega-terminus in 9,11-endoxy-10a-homo prostaglandin H2 with a p-fluorophenoxy group produces a compound (EP 171) with very high agonist potency at TP-receptors. 2...
  83. ncbi Differential effect of pH on thromboxane A2/prostaglandin H2 receptor agonist and antagonist binding in human platelets
    P R Mayeux
    Department of Cell and Molecular Pharmacology, and Experimental Therapeutics, Medical University of South Carolina, Charleston 29425
    J Biol Chem 266:13752-8. 1991
    The effects of changes in pH on the binding of agonists and antagonists to the human platelet thromboxane A2/prostaglandin H2 (TXA2/PGH2) receptor were determined...
  84. ncbi Characterization of thromboxane A2/prostaglandin H2 receptors and histamine H1 receptors in cultured guinea-pig tracheal smooth-muscle cells
    I Miki
    Pharmaceutical Research Laboratories, Kyowa Hakko Kogyo Co, Ltd, Shizuoka, Japan
    Biochim Biophys Acta 1137:107-15. 1992
    We characterized thromboxane A2/prostaglandin H2 (TXA2/PGH2) receptors and histamine H1 receptors in Guinea-pig cultured tracheal smooth-muscle cells (TSMC)...
  85. ncbi Comparison of antihypertensive and metabolic effects of losartan and losartan in combination with hydrochlorothiazide--a randomized controlled trial
    P Owens
    Blood Pressure Unit, Beaumont Hospital, Dublin, Ireland
    J Hypertens 18:339-45. 2000
    ..It also inhibits platelet agreggation through blockade of thromboxane A2/ prostaglandin H2 receptors, and has a uricosuric effect We determined the effect on ambulatory blood pressure (ABP) of 100 mg ..
  86. ncbi Characterization of thromboxane A2/prostaglandin H2 binding sites in guinea pig cardiac membrane preparations
    N Bowling
    Lilly Research Laboratories, Eli Lilly and Company, Lilly Corporate Center, Indianapolis, IN 46285
    J Mol Cell Cardiol 26:915-23. 1994
    Thromboxane A2 (TXA2) and prostaglandin H2 (PGH2) induce platelet aggregation and are potent vasoconstrictors, and they have been implicated in coronary vasospasm and myocardial infarction...
  87. ncbi Reduction by arachidonic acid of prostaglandin I2-induced cyclic AMP formation. Involvement of prostaglandins E2 and F2 alpha
    A Faili
    , , Paris, France
    Biochem Pharmacol 45:1815-20. 1993
    ..In addition, intracellular cyclic AMP levels and the degree of aggregation of platelets by arachidonic acid seem to be inversely correlated...
  88. ncbi Anti-peptide antibodies against the human blood platelet thromboxane A2/prostaglandin H2 receptor. Production, purification and characterization
    C Borg
    Department of Pharmacology, University of Illinois, Chicago 60607
    Biochem Pharmacol 45:2071-8. 1993
    Two anti-peptide antibodies have been raised against the human blood platelet thromboxane A2/prostaglandin H2 (TXA2/PGH2) receptor...
  89. ncbi Arachidonic acid elicits endothelium-dependent release from the rabbit aorta of a constrictor prostanoid resembling prostaglandin endoperoxides
    P J Pagano
    Department of Pharmacology, New York Medical College, Valhalla 10595
    Circ Res 69:396-405. 1991
    ..cyclooxygenase, when the endothelium of the perfused aorta was removed by rubbing, or when the thromboxane A2/prostaglandin H2 receptors of the vascular tissues used for bioassay were blocked with an antagonist (1 microM SQ29548), and ..
  90. ncbi Prostanoid and TP-receptors in atherothrombosis: is there a role for their antagonism?
    Chiara Giannarelli
    Atherothrombosis Research Unit, Cardiovascular Institute, Mount Sinai School of Medicine, New York, New York 10029, USA
    Thromb Haemost 104:949-54. 2010
    ....
  91. ncbi Short-term ANG II produces renal vasoconstriction independent of TP receptor activation and TxA2/isoprostane production
    Øyvind B Vågnes
    Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    Am J Physiol Renal Physiol 293:F860-7. 2007
    ....
  92. ncbi Phenol red is a thromboxane A2/prostaglandin H2 receptor antagonist in canine lingual arteries and human platelets
    S S Greenberg
    Louisiana State University, Department of Medicine, New Orleans
    J Pharmacol Exp Ther 268:1352-61. 1994
    ..The concentration of PR in many culture media is between 40 and 70 microM. The data support the conclusion that PR, in concentrations used as a pH indicator, is a selective antagonist of TPE receptors...
  93. ncbi Heterogeneity in filamentous actin content among individual human blood platelets
    A Oda
    Department of Surgery, Beth Israel Hospital, Boston, MA 02215
    Blood 79:920-7. 1992
    ..1) NBD-phallacidin binding increased with stimulation by ADP, U46619 (a prostaglandin H2 analogue), or collagen and paralleled shape change. (2) Epinephrine did not increase NBD-phallacidin binding...
  94. ncbi Ethanol-induced vasoconstriction is mediated via redox-sensitive cyclo-oxygenase-dependent mechanisms
    Alvaro Yogi
    Kidney Research Centre, Ottawa Hospital Research Institute, University of Ottawa, Ontario, Canada
    Clin Sci (Lond) 118:657-68. 2010
    ..micromol/l), AH6809 [PGF2alpha (prostaglandin F2alpha)] receptor antagonist, 10 micromol/l] or SQ29584 [PGH2(prostaglandin H2)/TXA2 (thromboxane A2) receptor antagonist, 3 micromol/l] inhibited ethanol-induced contraction in aortic ..
  95. ncbi Purification of rat brain, rabbit aorta, and human platelet thromboxane A2/prostaglandin H2 receptors by immunoaffinity chromatography employing anti-peptide and anti-receptor antibodies
    C Borg
    Department of Pharmacology, University of Illinois, Chicago 60612
    J Biol Chem 269:6109-16. 1994
    In the present study, a new polyclonal antibody (TxAb) was raised against native thromboxane A2 (TXA2)/prostaglandin H2 (PGH2) receptor protein...
  96. ncbi Desensitization of platelet thromboxane A2/prostaglandin H2 receptors by the mimetic U46619
    N Liel
    Department of Cell and Molecular Pharmacology, Medical University of South Carolina, Charleston
    J Pharmacol Exp Ther 247:1133-8. 1988
    Thromboxane A2 (TXA2) and prostaglandin H2 (PGH2) activate platelets through membrane receptors...
  97. ncbi Labeling of human platelet plasma membrane thromboxane A2/prostaglandin H2 receptors using SQB, a novel biotinylated receptor probe
    D Komiotis
    Department of Pharmacology, University of Illinois at Chicago 60612, USA
    Biochem Pharmacol 52:763-70. 1996
    ..d] imidazole-4'-pentanoyl]hydrazine (SQB) of the thromboxane A2/prostaglandin H2 (TXA2/PGH2) receptor antagonist [1S-[1 alpha,2 alpha(Z),3 alpha,4 alpha]]-7-[3-[[[[(1-oxocyclohexylpropyl)..
  98. ncbi Human platelet aggregation and shape change are coupled to separate thromboxane A2-prostaglandin H2 receptors
    G W Dorn
    Department of Medicine, University of Cincinnati, Ohio 45267 0542
    Am J Physiol 260:H327-34. 1991
    The relationships of human platelet thromboxane A2-prostaglandin H2 (TxA2/PGH2) receptor occupation as assessed by equilibrium binding of the TxA2/PGH2 agonist [125I]BOP to the functional responses of I-BOP-induced platelet shape change ..
  99. ncbi Thromboxane A2/prostaglandin H2 receptors in streptozotocin-induced diabetes: effects of insulin therapy in the rat
    T A Morinelli
    Department of Cell and Molecular Pharmacology, Medical University of South Carolina, Charleston 29425
    Prostaglandins 45:427-38. 1993
    Thromboxane A2 (TXA2) and prostaglandin H2 (PGH2) are potent vasoactive and proaggregatory agents whose synthesis has been shown to be elevated in diabetes mellitus...
  100. ncbi Subcellular localization of a thromboxane A2/prostaglandin H2 receptor antagonist binding site in human platelets
    D L Saussy
    Department of Cell and Molecular Pharmacology, Medical University of South Carolina, Charleston 29425
    Biochem Pharmacol 37:647-54. 1988
    The subcellular localization of a binding site for the competitive thromboxane A2/prostaglandin H2 (TXA2/PGH2) antagonist, 9,11-dimethylmethano-11,12-methano-16-(3-iodo-4- hydroxyphenyl)-13,14-dihydro-13-aza-15 alpha beta-omega-tetranor ..
  101. pmc Potentiation of aggregation and inhibition of adenylate cyclase in human platelets by prostaglandin E analogues
    J S Matthews
    Department of Pharmacology, University of Edinburgh, Scotland
    Br J Pharmacol 108:363-9. 1993
    ..In addition the necessity to consider potentiation of platelet aggregation in -relation to the clinical use of PGE analogues in man is emphasised...

Research Grants44

  1. Eicosanoid Regulation in Atherosclerosis: Involvement of Inducible NO Synthase
    David P Hajjar; Fiscal Year: 2010
    ..We, and others, have shown that 7NO modulates prostaglandin H2 synthase (PGHS, also known as cyclooxygenase) and alters eicosanoid production...
  2. Lipid Modification of Proteins by the PGH Synthases
    John A Oates; Fiscal Year: 2010
    ..This research will characterize the sites of post-translational modification of the PGHSs by their levuglandin products and will determine the effect of these modifications on the degradation of these important enzymes. ..
  3. Catalysis by Prostaglandin Endoperoxide H Synthases
    William L Smith; Fiscal Year: 2013
    ..also known as cyclooxygenases-1 and -2 (COX-1 and COX-2), catalyze the conversion of arachidonic acid (AA) to prostaglandin H2 (PGH2) in the committed step of prostaglandin (PG) biosynthesis...
  4. REGULATION OF RENAL FUNCTION AND BP BY THROMBOXANE
    Christopher S Wilcox; Fiscal Year: 2012
    ..These studies are focused on the roles of COX-1 vs. -2, and TP-R in mediating renal mechanism of homeostasis and their dysregulation during Ang II hypertension. ..
  5. SIGNAL TRANSDUCTION MECHANISMS AND AMNION PGE2 SYNTHESIS
    Leslie Myatt; Fiscal Year: 1993
    ..The opportunities for prevention of preterm labor at the biochemical level then become real...
  6. STRUCTURE AND MECHANISM OF PROSTAGLANDIN H2 SYNTHASE
    PATRICK LOLL; Fiscal Year: 2002
    ..PGHS catalyzes the conversion of arachidonic acid to prostaglandin H2 and this process is inhibited by nonsteroidal anti-inflammatory drugs (NSAIDs) such as aspirin, ibuprofen, ..
  7. Inhibition of novel molecular targets of prostaglandin formation for antitumor ac
    EMMANUELLE JOELLE MEUILLET; Fiscal Year: 2013
    ..The overall goal of our work is to develop novel agents for the improved treatment of colon cancer. ..
  8. REGULATION OF RENAL FUNCTION AND BP BY THROMBOXANE
    CHRISTOPHER WILCOX; Fiscal Year: 2002
    ....
  9. PGI & TXA SYNTHASES--MEMBRANE ANCHOR STRUCTURE/FUNCTION
    Ke He Ruan; Fiscal Year: 1999
    ..Ke-He Ruan. The title of the project is PGI & TXA Synthases: Membrane Anchor Structure/Function. Prostaglandin H2 (PGH2) is converted to Prostaglandin I2 (PGI2) via the catalytic action of Prostaglandin I2 synthase (PGIS)...
  10. ISOLATION/PURIFICATION MAMMALIAN PROSTACYCLIN SYNTHASE
    KRISHNAMOORTHY SANKARAN; Fiscal Year: 1992
    ..Attempts will be made to use the monoclonal antibodies developed in a large scale, single step purification procedure. The purified enzyme, the antibodies and the nucleic acid probes will be marketed Oxford Biomedical Research...
  11. CONFERENCE ON EICOSANOID LIPID MEDIATORS
    William Smith; Fiscal Year: 2001
    ..topics focuses on defining how COX-1 and -2 couple biochemically to upstream phospholipase A2 and downstream prostaglandin H2 (PGH2) metabolizing enzymes and on how different prostanoid receptors are involved in the actions of the COX ..
  12. MODELS OF ALZHEIMER'S DISEASE
    Katrin Andreasson; Fiscal Year: 2003
    ..In Aim 3, we will test the effect of a selective cox-2 inhibitor on the development of a pathological AD phenotype in single, bigenic and trigenic mice generated in Aims 1 and 2. ..
  13. VASOMOTOR CONTROL OF DESCENDING VASA RECTA
    THOMAS PALLONE; Fiscal Year: 2003
    ....
  14. IMAGING COX-2 GENE EXPRESSION IN INFLAMMATION AND TUMORS
    Harvey Herschman; Fiscal Year: 2009
    ..Studies with this mouse will correlate optical and microPET imaging technologies. ..
  15. Regulation of Prostaglandin E Synthases in Parturition
    William Ackerman; Fiscal Year: 2004
    ..We hope to support the hypothesis that, as in other systems, microsomal PGES is functionally linked to COX-2 in the generation of uterotonic POE2. ..
  16. SYNTHESIS OF PROSTAGLANDINS AND PROSTAGLANDIN ANALOGS
    Josef Fried; Fiscal Year: 1990
    ..Thus, from the known mechanism of leukotriene biosynthesis 7,7-DFAA could be a reversible inhibitor of 5-lipoxygenase while 10,10-DFAA would proceed as far as 5-HPETE, conversion to LTCA being blocked by the fluorines at C-10...
  17. PROSTAGLANDINS AND THROMBOXANE IN PERIODONTAL DISEASES
    Patrick Wong; Fiscal Year: 1980
    ..Further investigations of the release and ultimate fate of these vaoactive lipids will then be related to the possible physiological mechanism for the regulation of the inflammatory process and bone resorption in peridontal disease...
  18. Oxidants, eicosanoids, and endothelium in diabetes
    Richard Cohen; Fiscal Year: 2002
    ..abstract_text> ..
  19. GENERATION OF ARACHIDONATE FROM HUMAN PLATELETS
    Wai Cheung; Fiscal Year: 1990
    ..Information gathered from these studies will shed light on the mechanisms controlling the release of free arachidonic acid, which serves as the precursor of all eicosanoids, agents that play important roles in platelet functions...
  20. ISOLATION AND PURIFICATION OF PROSTAGLANDIN E ISOMERASE
    KRISHNAMOORTHY SANKARAN; Fiscal Year: 1993
    ....
  21. Lipid-Modification of Proteins in Alzheimer's Disease
    John Oates; Fiscal Year: 2006
    ..The immediate product of the cyclooxygenases is prostaglandin H2(PGH2), which rapidly rearranges in aqueous solution with approximately 20% being converted to levuglandin E2(..
  22. ENDOTHELIAL CONTROL OF ARTERIOLAR TONE IN HYPERTENSION
    Akos Koller; Fiscal Year: 2004
    ....
  23. Reactive gamma-Ketoaldehydes in Dementia
    L Roberts; Fiscal Year: 2008
    ..We will also explore a novel pharmacologic intervention, the ability of pyridoxamine to selectively prevent IsoK/NK adduction and mitigate the memory deficit in the mouse model. ..
  24. THE BIOLOGY OF PROGRESSIVE NEPHRON DESTRUCTION
    Raymond Harris; Fiscal Year: 2007
    ..abstract_text> ..
  25. Prostaglandin I synthase, Thromboxane A synthase & Prostaglandin E synthase
    Ke He Ruan; Fiscal Year: 2009
    ..PGIS, mPGES-1 and TXAS share a common substrate, prostaglandin H2 (PGH2), produced by COX-1 or -2, mainly occurring in the endoplasmic reticulum (ER) membrane...
  26. STRUCTURE & FUNCTION RELATIONSHIP: PROSTANOID RECEPTORS
    Ke He Ruan; Fiscal Year: 2008
    ..Determine the solution conformation of segments comprising the intracellular domains. ..
  27. Structural Biology of Eicosanoid Biosynthesis
    R Garavito; Fiscal Year: 2005
    ..Overexpression of rat prostacyclin synthase and divinylether synthase also make crystallographic studies on these two membrane-bound P450 enzymes tractable. ..
  28. Structure and function of family 1 glycosyltransferases
    R Garavito; Fiscal Year: 2006
    ..Mixing hybrid enzyme design with combinatorial biosynthesis could provide practical ways to produce new glycoconjugates of biomedical importance. ..
  29. Oxidative Stress Na Channel Gating and Arrhythmias
    L Roberts; Fiscal Year: 2007
    ..These studies should improve our understanding of the mechanisms linking oxidant injury and arrhythmias and have the potential to identify novel antiarrhythmic therapeutic strategies. [unreadable] [unreadable]..
  30. Thromboxane synthases-enzymology and gene regulation
    Lee Ho Wang; Fiscal Year: 2007
    ..by applicant): [unreadable] Thromboxane synthase (TXAS) is a "non-classical" cytochrome P450 that converts prostaglandin H2 to thromboxane A2 (TXA2), a potent inducer of vasoconstriction and platelet aggregation...
  31. NSAID and COX/PG Metabolism and Colorectal Cancer
    Cornelia Ulrich; Fiscal Year: 2009
    ..Ultimately, such information on the metabolic variation will be useful in optimizing NSAIDs and NSAID regimens and will allow individual tailoring of chemoprevention. ..
  32. Skeletal Muscle Microcirculation in Obese Zucker Rats
    Jefferson Frisbee; Fiscal Year: 2009
    ..abstract_text> ..
  33. Role of Ang-(1-7) in normal and hypertensive pregnancy
    K Brosnihan; Fiscal Year: 2003
    ..The major goal of these studies is to understand the contribution of the vasodilator component of the RAAS to blood pressure regulation in normal pregnancy. ..
  34. POLYMORPHISMS IN PG/COX PATHWAY AND COLORECTAL POLYPS
    Cornelia Ulrich; Fiscal Year: 2003
    ..Finally, we plan to develop a model for integrating information on genetic variability at multiple points in this metabolic pathway. ..
  35. EICOSANOIDS AND NO IN CEREBROVASCULAR THROMBOSIS
    Kenneth Wu; Fiscal Year: 2003
    ..It is intended that this program will enable several laboratories to work individually and together to achieve the highest degree of innovating and productivity. ..
  36. Genetic Study of Prostaglandin Synthesis/EGFR and Risk of Colorectal Neoplasia
    Cornelia Ulrich; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  37. Arachidonic Acid Metabolism in Blood and Vascular Cells
    Jun Yang Liou; Fiscal Year: 2006
    ..Information provided by studies from this renewal application will have great impact on understanding the fundamental cytoprotection process and offer an excellent opportunity for developing new therapeutic agents. ..
  38. Prostaglandin Synthesis, Genetics and Colorectal Cancer
    Cornelia M Ulrich; Fiscal Year: 2010
    ..They will also advance tailoring of chemoprevention in a way that maximizes benefit and minimizes toxicity. ..
  39. Training Program in Molecular Medicine
    KATHLEEN BROSNIHAN; Fiscal Year: 2008
    ..This training grant will allow continued growth and recruitment of the most competitive applicants and minorities into the Program. [unreadable] [unreadable]..
  40. Phospholipid and Arachidonic Acid Signaling in Epilepsy
    Nicolas Bazan; Fiscal Year: 2007
    ..abstract_text> ..
  41. Modeling Folate, One-Carbon Metabolism & DNA Methylation
    Cornelia Ulrich; Fiscal Year: 2007
    ..We plan to make the model generally available to the research community, and anticipate that it will become a useful tool that can be used as an adjunct to experimental investigations and as an aid to study design. ..
  42. Metabolism and Carcinogenicity of Heterocyclic Amines
    TERRY ZENSER; Fiscal Year: 2007
    ..Determine effect of DSS-induced colitis inflammation) in IQ carcinogenicity. The long-term goal is to understand the role of RNOS on HCA carcinogenesis. ..
  43. Exercise effects on oxidative damage among women
    Cornelia Ulrich; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  44. Prostaglandins: Kinins and Angiotensin Interactions
    JOHN MC GIFF; Fiscal Year: 2009
    ..3) Heme oxygenase activity and suppression of EET and 20-HETE synthesis. 4) Activation of peroxisome-proliferator-activated receptor alpha by Fibrates with stimulation of EET synthesis. ..