disulfides

Summary

Summary: Chemical groups containing the covalent disulfide bonds -S-S-. The sulfur atoms can be bound to inorganic or organic moieties.

Top Publications

  1. ncbi Protein disulfide isomerase: a critical evaluation of its function in disulfide bond formation
    Feras Hatahet
    Department of Biochemistry, University of Oulu, Oulu, Finland
    Antioxid Redox Signal 11:2807-50. 2009
  2. ncbi Integrated circuits based on bilayer MoS₂ transistors
    Han Wang
    Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States
    Nano Lett 12:4674-80. 2012
  3. ncbi Two endoplasmic reticulum PDI peroxidases increase the efficiency of the use of peroxide during disulfide bond formation
    Van Dat Nguyen
    Department of Biochemistry, University of Oulu, Linnanmaa campus, 90570 Oulu, Finland
    J Mol Biol 406:503-15. 2011
  4. ncbi Protein disulfide isomerase
    Bonney Wilkinson
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Biochim Biophys Acta 1699:35-44. 2004
  5. ncbi Multiple ways to make disulfides
    Neil J Bulleid
    Institute of Molecular, Cellular and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
    Trends Biochem Sci 36:485-92. 2011
  6. pmc Recycling of peroxiredoxin IV provides a novel pathway for disulphide formation in the endoplasmic reticulum
    Timothy J Tavender
    College of Medical, Veterinary and Life Sciences, Institute of Molecular Cell and Systems Biology, University of Glasgow, Glasgow, UK
    EMBO J 29:4185-97. 2010
  7. pmc Generating disulfides enzymatically: reaction products and electron acceptors of the endoplasmic reticulum thiol oxidase Ero1p
    Einav Gross
    Department of Structural Biology, The Weizmann Institute of Science, Rehovot 76100, Israel
    Proc Natl Acad Sci U S A 103:299-304. 2006
  8. ncbi Real-time imaging of the intracellular glutathione redox potential
    Marcus Gutscher
    Redox Regulation Research Group, German Cancer Research Center DKFZ A160, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
    Nat Methods 5:553-9. 2008
  9. ncbi Tuned Escherichia coli as a host for the expression of disulfide-rich proteins
    Gustavo Salinas
    Catedra de Inmunologia, Facultad de Quimica Facultad de Ciencias, Instituto de Higiene, Universidad de la Republica, Montevideo, Uruguay
    Biotechnol J 6:686-99. 2011
  10. ncbi Subunit arrangement and function in NMDA receptors
    Hiroyasu Furukawa
    Department of Biochemistry and Molecular Biophysics, Columbia University, 650 West 168th Street, New York, New York 10032, USA
    Nature 438:185-92. 2005

Research Grants

Detail Information

Publications386 found, 100 shown here

  1. ncbi Protein disulfide isomerase: a critical evaluation of its function in disulfide bond formation
    Feras Hatahet
    Department of Biochemistry, University of Oulu, Oulu, Finland
    Antioxid Redox Signal 11:2807-50. 2009
    ....
  2. ncbi Integrated circuits based on bilayer MoS₂ transistors
    Han Wang
    Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States
    Nano Lett 12:4674-80. 2012
    ..Both enhancement-mode and depletion-mode transistors were fabricated thanks to the use of gate metals with different work functions...
  3. ncbi Two endoplasmic reticulum PDI peroxidases increase the efficiency of the use of peroxide during disulfide bond formation
    Van Dat Nguyen
    Department of Biochemistry, University of Oulu, Linnanmaa campus, 90570 Oulu, Finland
    J Mol Biol 406:503-15. 2011
    ..Hence, GPx7 and GPx8 may represent a novel route for the productive use of peroxide produced by Ero1α during disulfide bond formation...
  4. ncbi Protein disulfide isomerase
    Bonney Wilkinson
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Biochim Biophys Acta 1699:35-44. 2004
    ..reticulum and in the bacterial periplasm, an elaborate system of chaperones and folding catalysts ensure that disulfides connect the proper cysteines and that the folding protein does not make improper interactions...
  5. ncbi Multiple ways to make disulfides
    Neil J Bulleid
    Institute of Molecular, Cellular and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
    Trends Biochem Sci 36:485-92. 2011
    Our concept of how disulfides form in proteins entering the secretory pathway has changed dramatically in recent years...
  6. pmc Recycling of peroxiredoxin IV provides a novel pathway for disulphide formation in the endoplasmic reticulum
    Timothy J Tavender
    College of Medical, Veterinary and Life Sciences, Institute of Molecular Cell and Systems Biology, University of Glasgow, Glasgow, UK
    EMBO J 29:4185-97. 2010
    ..Oxidation of PDI by PrxIV may therefore increase efficiency of disulphide formation by Ero1 and also allows disulphide formation via alternative sources of H(2)O(2)...
  7. pmc Generating disulfides enzymatically: reaction products and electron acceptors of the endoplasmic reticulum thiol oxidase Ero1p
    Einav Gross
    Department of Structural Biology, The Weizmann Institute of Science, Rehovot 76100, Israel
    Proc Natl Acad Sci U S A 103:299-304. 2006
    ..These findings provide insight into mechanisms for regenerating oxidized Ero1p and maintaining disulfide bond formation under anaerobic conditions in the endoplasmic reticulum...
  8. ncbi Real-time imaging of the intracellular glutathione redox potential
    Marcus Gutscher
    Redox Regulation Research Group, German Cancer Research Center DKFZ A160, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
    Nat Methods 5:553-9. 2008
    ..It facilitated the observation of redox changes associated with growth factor availability, cell density, mitochondrial depolarization, respiratory burst activity and immune receptor stimulation...
  9. ncbi Tuned Escherichia coli as a host for the expression of disulfide-rich proteins
    Gustavo Salinas
    Catedra de Inmunologia, Facultad de Quimica Facultad de Ciencias, Instituto de Higiene, Universidad de la Republica, Montevideo, Uruguay
    Biotechnol J 6:686-99. 2011
    ..coli...
  10. ncbi Subunit arrangement and function in NMDA receptors
    Hiroyasu Furukawa
    Department of Biochemistry and Molecular Biophysics, Columbia University, 650 West 168th Street, New York, New York 10032, USA
    Nature 438:185-92. 2005
    ....
  11. ncbi Disulfide bonds as switches for protein function
    Philip J Hogg
    Centre for Vascular Research, University of New South Wales, and Department of Haematology, Prince of Wales Hospital, NSW, Australia
    Trends Biochem Sci 28:210-4. 2003
    ..The function of some secreted soluble proteins and cell-surface receptors is controlled by cleavage of one or more of their disulfide bonds; this cleavage is mediated by catalysts or facilitators that are specific for their substrate...
  12. ncbi Modulation of cellular disulfide-bond formation and the ER redox environment by feedback regulation of Ero1
    Carolyn S Sevier
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cell 129:333-44. 2007
    ..the secretory pathway is catalyzed by Ero1p, which generates disulfide bonds de novo, and Pdi1p, which transfers disulfides to substrate proteins...
  13. pmc The role of glutathione in disulphide bond formation and endoplasmic-reticulum-generated oxidative stress
    Seema Chakravarthi
    The Faculty of Life Sciences, University of Manchester, The Michael Smith Building, Oxford Road, Manchester M13 9PT, UK
    EMBO Rep 7:271-5. 2006
    ..In this review, we discuss the arguments for and against a role for glutathione in facilitating disulphide-bond formation and consider its role in protecting the cell from endoplasmic-reticulum-generated oxidative stress...
  14. pmc Pre-expression of a sulfhydryl oxidase significantly increases the yields of eukaryotic disulfide bond containing proteins expressed in the cytoplasm of E.coli
    Van Dat Nguyen
    Department of Biochemistry, Linnanmaa campus, University of Oulu, 90570 Oulu, Finland
    Microb Cell Fact 10:1. 2011
    ..We have recently shown that it is possible to produce large amounts of prokaryotic disulfide bond containing proteins in the cytoplasm of wild-type bacteria such as E. coli by the introduction of catalysts for both of these processes...
  15. ncbi Predicting disulfide connectivity from protein sequence using multiple sequence feature vectors and secondary structure
    Jiangning Song
    Advanced Computational Modelling Centre, The University of Queensland, Brisbane, QLD 4072, Australia
    Bioinformatics 23:3147-54. 2007
    ..Therefore, there is a great need to develop computational methods capable of accurately predicting disulfide connectivity patterns in proteins that could have potentially important applications...
  16. ncbi The physiological functions of mammalian endoplasmic oxidoreductin 1: on disulfides and more
    Thomas Ramming
    Division of Molecular and Systems Toxicology, Department of Pharmaceutical Sciences, University of Basel, Switzerland
    Antioxid Redox Signal 16:1109-18. 2012
    ..The conserved, ER-resident endoplasmic oxidoreductin 1 (Ero1) sulfhydryl oxidases that reduce molecular oxygen to generate an active-site disulfide represent one of these pathways. In mammals, two family members exist, Ero1α and Ero1β...
  17. ncbi Disulfide by Design: a computational method for the rational design of disulfide bonds in proteins
    Alan A Dombkowski
    Institute of Environmental Health Sciences, Wayne State University, Detroit, MI 48201, USA
    Bioinformatics 19:1852-3. 2003
    ..pairs having the appropriate geometric characteristics for disulfide formation and provides automated generation of modified PDB files including modeled disulfides. Validation demonstrates a high level of accuracy for the algorithm.
  18. pmc Balanced Ero1 activation and inactivation establishes ER redox homeostasis
    Sunghwan Kim
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Cell Biol 196:713-25. 2012
    ....
  19. ncbi Disulfide bond formation system in Escherichia coli
    Kenji Inaba
    Division of Protein Chemistry, Post Genome Science Center, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    J Biochem 146:591-7. 2009
    ..To a great extent, the molecular mechanisms of the Dsb enzyme system in E. coli have been elucidated, and are applicable to the study of protein disulfide formation systems in other organisms...
  20. pmc DiANNA: a web server for disulfide connectivity prediction
    F Ferre
    Department of Biology, Boston College, Chestnut Hill, MA 02467, USA
    Nucleic Acids Res 33:W230-2. 2005
    ..Our novel neural network-based approach achieves results that are comparable and in some cases better than the current state-of-the-art methods...
  21. pmc Low reduction potential of Ero1alpha regulatory disulphides ensures tight control of substrate oxidation
    Karl M Baker
    Faculty of Life Sciences, University of Manchester, Manchester, UK
    EMBO J 27:2988-97. 2008
    ....
  22. pmc Disulphide production by Ero1α-PDI relay is rapid and effectively regulated
    Christian Appenzeller-Herzog
    Department of Biology, University of Copenhagen, Copenhagen, Denmark
    EMBO J 29:3318-29. 2010
    ..Instead, our data suggest that a dynamic equilibrium between Ero1- and glutathione disulphide-mediated oxidation of PDIs constitutes an important element of ER redox homeostasis...
  23. pmc Mechanisms of oxidative protein folding in the bacterial cell envelope
    Hiroshi Kadokura
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama, Ikoma, Nara, Japan
    Antioxid Redox Signal 13:1231-46. 2010
    ..We review here the marked progress in this field and discuss important questions that remain for future studies...
  24. ncbi Activation of the OxyR transcription factor by reversible disulfide bond formation
    M Zheng
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 279:1718-21. 1998
    ..The redox potential of OxyR was determined to be -185 millivolts, ensuring that OxyR is reduced in the absence of stress. These results represent an example of redox signaling through disulfide bond formation and reduction...
  25. pmc SCRATCH: a protein structure and structural feature prediction server
    J Cheng
    Institute for Genomics and Bioinformatics, University of California Irvine, CA, USA
    Nucleic Acids Res 33:W72-6. 2005
    ..The user simply provides an amino acid sequence and selects the desired predictions, then submits to the server. Results are emailed to the user. The server is available at http://www.igb.uci.edu/servers/psss.html...
  26. pmc Locations of the beta1 transmembrane helices in the BK potassium channel
    Guoxia Liu
    Division of Cardiology, Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 105:10727-32. 2008
    ..TM1 and TM2 are in position to contribute, in concert with the extracellular loop and the intracellular N- and C-terminal tails of beta1, to the modulation of BK channel function...
  27. pmc Location of modulatory beta subunits in BK potassium channels
    Guoxia Liu
    Division of Cardiology, Department of Medicine, LaGuardia Community College, Long Island City, NY 11101, USA
    J Gen Physiol 135:449-59. 2010
    ..Thus, each beta lies between and can interact with the voltage-sensing domains of two adjacent alpha subunits...
  28. ncbi Simian Virus 40 depends on ER protein folding and quality control factors for entry into host cells
    Mario Schelhaas
    Institute of Biochemistry, Schafmattstrasse 18, ETH Hoenggerberg, ETH Zurich, CH 8093, Zurich, Switzerland
    Cell 131:516-29. 2007
    ..ERp57 isomerizes specific interchain disulfides connecting the major capsid protein, VP1, to a crosslinked network of neighbors, thus uncoupling about 12 of 72 ..
  29. pmc Agonist binding evokes extensive conformational changes in the extracellular domain of the ATP-gated human P2X1 receptor ion channel
    Jonathan A Roberts
    Department of Cell Physiology and Pharmacology, University of Leicester, Leicester LE1 9HN, United Kingdom
    Proc Natl Acad Sci U S A 109:4663-7. 2012
    ....
  30. pmc Generating disulfides with the Quiescin-sulfhydryl oxidases
    Erin J Heckler
    Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19711, USA
    Biochim Biophys Acta 1783:567-77. 2008
    ..Finally, this review identifies important unresolved questions concerning this ancient family of sulfhydryl oxidases...
  31. ncbi Locking the beta3 integrin I-like domain into high and low affinity conformations with disulfides
    Bing Hao Luo
    CBR Institute fo Biomedical Research and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 279:10215-21. 2004
    ..e. that axial movement in the C-terminal direction of the alpha7-helix is linked to rearrangement of the I-like domain metal ion-dependent adhesion site into a high affinity conformation...
  32. ncbi Disulfide formation in the ER and mitochondria: two solutions to a common process
    Jan Riemer
    Cell Biology, University of Kaiserslautern, Erwin Schrödinger Strasse 13, 67663 Kaiserslautern, Germany
    Science 324:1284-7. 2009
    ..Whereas the machineries of ER and mitochondria both form disulfides between cysteine residues, they do not share evolutionary origins and exhibit distinct mechanistic properties...
  33. ncbi The FAD- and O(2)-dependent reaction cycle of Ero1-mediated oxidative protein folding in the endoplasmic reticulum
    Benjamin P Tu
    Howard Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA 94143, USA
    Mol Cell 10:983-94. 2002
    ..Thus Ero1p directly couples disulfide formation to the consumption of molecular oxygen, but its activity is modulated by free lumenal FAD levels, potentially linking disulfide formation to a cell's nutritional or metabolic status...
  34. ncbi Improving the prediction of disulfide bonds in Eukaryotes with machine learning methods and protein subcellular localization
    Castrense Savojardo
    Biocomputing Group, University of Bologna, CIRI Life Science and Health Technologies and Department of Biology, Via San Giacomo 9 2, Bologna, Italy
    Bioinformatics 27:2224-30. 2011
    ..Several methods are available to predict cysteine-bonding state and connectivity patterns. However, none of them takes into consideration the relevance of protein subcellular localization...
  35. pmc Direct observation of disulfide isomerization in a single protein
    Jorge Alegre-Cebollada
    Department of Biological Sciences, Columbia University, Northwest Corner Building, 550 West 120 Street, New York, New York 10027, USA
    Nat Chem 3:882-7. 2011
    ..We used single-molecule force spectroscopy to monitor the cleavage of the disulfides, which extends the polypeptide by a magnitude unambiguously associated with each reaction pathway...
  36. pmc Molecular bases of cyclic and specific disulfide interchange between human ERO1alpha protein and protein-disulfide isomerase (PDI)
    Shoji Masui
    Division of Protein Chemistry, Post Genome Science Center, Medical Institute of Bioregulation, Kyushu University, Higashi ku, Fukuoka, Japan
    J Biol Chem 286:16261-71. 2011
    ..The potential ability of ERp44 to inhibit ERO1α activity may suggest its physiological role in ER redox and protein homeostasis...
  37. ncbi Identification of a protein required for disulfide bond formation in vivo
    J C Bardwell
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115
    Cell 67:581-9. 1991
    ..The purified DsbA protein is capable of reducing the disulfide bonds of insulin, an activity that it shares with these disulfide oxidoreductases. Our results suggest that disulfide bond formation is facilitated by DsbA in vivo...
  38. pmc Role of mutant SOD1 disulfide oxidation and aggregation in the pathogenesis of familial ALS
    Celeste M Karch
    Department of Neuroscience, McKnight Brain Institute, University of Florida, Gainesville, FL 32610 0235, USA
    Proc Natl Acad Sci U S A 106:7774-9. 2009
    ..Within the final stages of disease, abnormalities in the oxidation of a normal intramolecular disulfide bond in mutant SOD1 facilitate the aggregation of mutant protein...
  39. ncbi The human PDI family: versatility packed into a single fold
    Christian Appenzeller-Herzog
    Department of Molecular Biology, Universitetsparken 13, University of Copenhagen, DK 2100 Copenhagen Ø, Denmark
    Biochim Biophys Acta 1783:535-48. 2008
    ..Here, we primarily explore the diversity of cellular functions described for the human PDIs...
  40. pmc Intracellular catalysis of disulfide bond formation by the human sulfhydryl oxidase, QSOX1
    Seema Chakravarthi
    Faculty of Life Sciences, Michael Smith Building, University of Manchester, Manchester M13 9PT, UK
    Biochem J 404:403-11. 2007
    ..Overexpression of hQSOX1a suppresses the lethality of a complete deletion of ERO1 (endoplasmic reticulum oxidase 1) in yeast and restores disulfide bond formation, as assayed by the folding of the secretory protein carboxypeptidase Y...
  41. ncbi A disulfide relay system in the intermembrane space of mitochondria that mediates protein import
    Nikola Mesecke
    Institute für Physiologische Chemie, Universitat Munchen, Germany
    Cell 121:1059-69. 2005
    ..We suggest that this process reflects the evolutionary origin of the IMS from the periplasmic space of the prokaryotic ancestors of mitochondria...
  42. pmc Identification and characterization of disulfide bonds in proteins and peptides from tandem MS data by use of the MassMatrix MS/MS search engine
    Hua Xu
    Department of Chemistry, The Ohio State University, Columbus, Ohio 43210, USA
    J Proteome Res 7:138-44. 2008
    ..0) during digestion, only one nonnative disulfide bond was observed. The MassMatrix algorithm offers an additional approach for the discovery of disulfide bond from tandem mass spectrometry data...
  43. ncbi Allosteric disulfide bonds
    Bryan Schmidt
    Centre for Vascular Research, University of New South Wales and Department of Haematology, Prince of Wales Hospital, Sydney, New South Wales 2052, Australia
    Biochemistry 45:7429-33. 2006
    ..These bonds can be thought of as allosteric disulfides. To better define the properties of allosteric disulfides, we have analyzed the geometry and dihedral strain of ..
  44. ncbi Disulfides as redox switches: from molecular mechanisms to functional significance
    Merridee A Wouters
    Structural and Computational Biology Division, Victor Chang Cardiac Research Institute, Sydney, Australia
    Antioxid Redox Signal 12:53-91. 2010
    ..Redox biologists have been redefining the role of disulfides over the last 30-40 years. Disulfides are now known to form in the cytosol under conditions of oxidative stress...
  45. pmc Peroxiredoxin IV protects cells from oxidative stress by removing H2O2 produced during disulphide formation
    Timothy J Tavender
    The Faculty of Life Sciences, The Michael Smith Building, University of Manchester, Oxford Road, Manchester, M13 9PT, UK
    J Cell Sci 123:2672-9. 2010
    ..These findings provide the first evidence for an enzymatic mechanism that facilitates peroxide removal from the ER, and show that the oxidation status of PrxIV acts as a marker for ER oxidative stress...
  46. pmc Disulfide bond isomerization in prokaryotes
    Stefan Gleiter
    Howard Hughes Medical Institute, University of Michigan, Ann Arbor, MI 48109 1048, USA
    Biochim Biophys Acta 1783:530-4. 2008
    ..This review discusses the current knowledge about disulfide isomerization in prokaryotes...
  47. pmc Selective redox regulation of cytokine receptor signaling by extracellular thioredoxin-1
    Ulla Schwertassek
    Redox Regulation Research Group, German Cancer Research Center DKFZ A160, Heidelberg, Germany
    EMBO J 26:3086-97. 2007
    ..Furthermore, we confirm that Trx1 affects CD30-dependent changes in lymphocyte effector function. Thus, we conclude that receptor-ligand signaling interactions can be selectively regulated by an extracellular redox catalyst...
  48. pmc DISULFIND: a disulfide bonding state and cysteine connectivity prediction server
    Alessio Ceroni
    Machine Learning and Neural Networks Group, Universita degli Studi di Firenze, Dipartimento di Sistemi e Informatica, Via di Santa Marta 3, 50139 Firenze, Italy
    Nucleic Acids Res 34:W177-81. 2006
    ..The output is a simple visualization of the assigned bonding state (with confidence degrees) and the most likely connectivity patterns. The server is available at http://disulfind.dsi.unifi.it/...
  49. pmc Cross-linking of rotavirus outer capsid protein VP7 by antibodies or disulfides inhibits viral entry
    Scott T Aoki
    Laboratory of Molecular Medicine, Children s Hospital, 3 Blackfan Circle, Boston, MA 02115, USA
    J Virol 85:10509-17. 2011
    ..We conclude that dissociation of the VP7 trimer is an essential step in viral penetration into cells...
  50. ncbi Picomole-level mapping of protein disulfides by mass spectrometry following partial reduction and alkylation
    Susan F Foley
    Biogen Idec, 14 Cambridge Center, Cambridge, MA 02142, USA
    Anal Biochem 377:95-104. 2008
    ..They will also provide a means to obtain important information for structure-function studies and to ensure correct protein folding and batch-to-batch consistency in commercially produced recombinant proteins...
  51. pmc Ligand-independent signaling by disulfide-crosslinked dimers of the p75 neurotrophin receptor
    Marçal Vilar
    Division of Molecular Neurobiology, Department of Neuroscience, Karolinska Institute, 17177 Stockholm, Sweden
    J Cell Sci 122:3351-7. 2009
    ..Together, these results support a conformational model of p75(NTR) activation by neurotrophins, and reveal a genetic approach to generate gain-of-function receptor variants with distinct functional profiles...
  52. ncbi Extracellular disulfide exchange and the regulation of cellular function
    Peter A Jordan
    School of Animal and Microbial Sciences, University of Reading, Whiteknights, Reading, United Kingdom
    Antioxid Redox Signal 8:312-24. 2006
    ..This review summarizes current knowledge of the extracellular regulation of disulfide exchange and the implications of this in the regulation of cell function...
  53. ncbi Production of disulfide-bonded proteins in Escherichia coli
    Mehmet Berkmen
    New England Biolabs, 240 County Road, Ipswich, MA 01938, USA
    Protein Expr Purif 82:240-51. 2012
    ..The purpose of this review is to familiarize the researcher with the biological principles involved in the formation of disulfide-bonded proteins with the hope of guiding the scientist in choosing the optimum expression system...
  54. pmc Calcium as a crucial cofactor for low density lipoprotein receptor folding in the endoplasmic reticulum
    Florentina Pena
    Cellular Protein Chemistry, Bijvoet Center for Biomolecular Research, Utrecht University, 3584 CH Utrecht, The Netherlands
    J Biol Chem 285:8656-64. 2010
    ..Coevolution of protein folding with the high calcium concentration in the ER may be the basis for the need for this cation throughout the folding process even though calcium is only stably integrated in native repeats at a later stage...
  55. pmc Human IgG2 antibodies display disulfide-mediated structural isoforms
    Jette Wypych
    Department of Analytical Sciences, Amgen Inc, Thousand Oaks, California 91320, USA
    J Biol Chem 283:16194-205. 2008
    ..These findings indicate that disulfide structural heterogeneity is a naturally occurring feature of antibodies belonging to the human IgG2 subclass...
  56. ncbi Biochemical basis of oxidative protein folding in the endoplasmic reticulum
    B P Tu
    Howard Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94143, USA
    Science 290:1571-4. 2000
    ..This kinetic shuttling of oxidizing equivalents could allow the ER to support rapid disulfide formation while maintaining the ability to reduce and rearrange incorrect disulfide bonds...
  57. pmc Structural evidence for loose linkage between ligand binding and kinase activation in the epidermal growth factor receptor
    Chafen Lu
    Immune Disease Institute and Department of Pathology, Harvard Medical School, 3 Blackfan Circle, Boston, MA 02115, USA
    Mol Cell Biol 30:5432-43. 2010
    ..These results suggest that linkage between ligand-induced dimerization and tyrosine kinase activation is much looser than was previously envisioned...
  58. pmc Arsenate reductase, mycothiol, and mycoredoxin concert thiol/disulfide exchange
    Efrén Ordóñez
    Department of Molecular Biology, Area of Microbiology, University of Leon, 24071 Leon, Spain
    J Biol Chem 284:15107-16. 2009
    ..With the in vitro reconstitution of this novel electron transfer pathway, we have paved the way for the study of redox mechanisms in actinobacteria...
  59. pmc Disulfide-reduced ALS variants of Cu, Zn superoxide dismutase exhibit increased populations of unfolded species
    Can Kayatekin
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    J Mol Biol 398:320-31. 2010
    ....
  60. ncbi Sulfhydryl oxidases: emerging catalysts of protein disulfide bond formation in eukaryotes
    Colin Thorpe
    Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA
    Arch Biochem Biophys 405:1-12. 2002
    ..domain and a small FAD-binding domain homologous to the yeast ERV1p protein to oxidize sulfhydryl groups to disulfides with the reduction of oxygen to hydrogen peroxide...
  61. pmc RsrA, an anti-sigma factor regulated by redox change
    J G Kang
    Department of Microbiology, College of Natural Sciences, and Research Center for Molecular Microbiology, Seoul National University, Seoul 151 742, Korea
    EMBO J 18:4292-8. 1999
    ..Thioredoxin was able to reduce oxidized RsrA, suggesting that sigma(R), RsrA and the thioredoxin system comprise a novel feedback homeostasis loop that senses and responds to changes in the intracellular thiol-disulfide redox balance...
  62. ncbi A secreted disulfide catalyst controls extracellular matrix composition and function
    Tal Ilani
    Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel
    Science 341:74-6. 2013
    ..We developed an inhibitory monoclonal antibody against QSOX1 that could modulate ECM properties and undermine cell migration. ..
  63. ncbi DSB proteins and bacterial pathogenicity
    Begoña Heras
    The University of Queensland, Institute for Molecular Bioscience, Queensland 4072, Australia
    Nat Rev Microbiol 7:215-25. 2009
    ..Although our current knowledge of this machinery stems largely from Escherichia coli K-12, this view must now be adjusted to encompass the wider range of disulphide catalytic systems present in bacteria...
  64. ncbi Low-density lipoprotein receptor structure and folding
    J Gent
    Department of Bio organic Chemistry 1, Utrecht University, Padualaan 8, 3584, CH Utrecht, The Netherlands
    Cell Mol Life Sci 61:2461-70. 2004
    ..In this review, we will elaborate on the structural aspects of the LDLR and its folding pathway and compare it to other LDLR family members...
  65. ncbi Mitochondrial disulfide bond formation is driven by intersubunit electron transfer in Erv1 and proofread by glutathione
    Melanie Bien
    Institut fur Zellbiologie, Universitat Kaiserslautern, Erwin Schrödinger Strasse 13, 67663 Kaiserslautern, Germany
    Mol Cell 37:516-28. 2010
    ..The generation of these side products is efficiently counteracted by reduced glutathione. Thus, our findings suggest a role for a glutathione-dependent proofreading during oxidative protein folding by the mitochondrial disulfide relay...
  66. pmc Vitamin K epoxide reductase contributes to protein disulfide formation and redox homeostasis within the endoplasmic reticulum
    Lori A Rutkevich
    Department of Biochemistry, University of Toronto, Toronto, ON, Canada
    Mol Biol Cell 23:2017-27. 2012
    ..In contrast, no involvement of QSOX1 in ER oxidative processes could be detected. These findings establish VKOR as a significant contributor to disulfide bond formation within the ER...
  67. pmc Photonic activation of disulfide bridges achieves oriented protein immobilization on biosensor surfaces
    Maria Teresa Neves-Petersen
    NanoBiotechnology Group, Department of Physics and Nanotechnology, Aalborg University, Aalborg, Denmark
    Protein Sci 15:343-51. 2006
    ....
  68. ncbi Synthesis of large-area MoS2 atomic layers with chemical vapor deposition
    Yi Hsien Lee
    Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, Taiwan
    Adv Mater 24:2320-5. 2012
    ..Optical, microscopic and electrical measurements suggest that the synthetic process leads to the growth of MoS(2) monolayer. The TEM images verify that the synthesized MoS(2) sheets are highly crystalline...
  69. ncbi Disulfide bond formation network in the three biological kingdoms, bacteria, fungi and mammals
    Yoshimi Sato
    Division of Protein Chemistry, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    FEBS J 279:2262-71. 2012
    ..Accumulating knowledge about disulfide bond formation networks widely distributed throughout the biological kingdom has significantly advanced our understanding of the cellular mechanisms dedicated to protein quality control...
  70. ncbi Gain of function in an ERV/ALR sulfhydryl oxidase by molecular engineering of the shuttle disulfide
    Elvira Vitu
    Department of Structural Biology, Weizmann Institute of Science, Rehovot 76100, Israel
    J Mol Biol 362:89-101. 2006
    ....
  71. pmc Overexpression and purification of U24 from human herpesvirus type-6 in E. coli: unconventional use of oxidizing environments with a maltose binding protein-hexahistine dual tag to enhance membrane protein yield
    Andrew R Tait
    Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, BC, V6T 1Z1, Canada
    Microb Cell Fact 10:51. 2011
    ....
  72. ncbi Identification of cysteines involved in S-nitrosylation, S-glutathionylation, and oxidation to disulfides in ryanodine receptor type 1
    Paula Aracena-Parks
    Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 281:40354-68. 2006
    ..In summary, we have identified a discrete subset of cysteines that are likely to be involved in the functional response of RyR1 to different redox modifications (S-nitrosylation, S-glutathionylation, and oxidation to disulfides).
  73. ncbi The role of the hinge loop in domain swapping. The special case of bovine seminal ribonuclease
    Delia Picone
    Dipartimento di Chimica, Universita Federico II di Napoli, Via Cynthia, 80126, Napoli, Italy
    J Biol Chem 280:13771-8. 2005
    ..the major role of Pro19 resides in directing the assembly of the non-covalent dimer, the species produced by selective reduction of the interchain disulfides and considered responsible for the special biological functions of BS-RNase.
  74. pmc SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm
    Julie Lobstein
    New England Biolabs, Ipswich, MA 01938, USA
    Microb Cell Fact 11:56. 2012
    ..Thus, new protein expression strains must be engineered and the parameters involved in producing disulfide bonded proteins must be understood...
  75. pmc CysView: protein classification based on cysteine pairing patterns
    Johann Lenffer
    Institute for Infocomm Research, 21 Heng Mui Keng Terrace, 119613 Singapore
    Nucleic Acids Res 32:W350-5. 2004
    ..CysView can be used to support distant homology between proteins. CysView is publicly available at http://research.i2r.a-star.edu.sg/CysView/...
  76. ncbi A quantitative description of in vitro assembly of human papillomavirus 16 virus-like particles
    Santanu Mukherjee
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    J Mol Biol 381:229-37. 2008
    ..2 disassembled slower than pH 5.2, 6.2, or 8.2 VLPs. We hypothesize that pH 7.2 assembly reactions lead to formation of particles with conformationally different interactions...
  77. ncbi Role of disulfide bonds in Acrp30/adiponectin structure and signaling specificity. Different oligomers activate different signal transduction pathways
    Tsu Shuen Tsao
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    J Biol Chem 278:50810-7. 2003
    ..Thus, trimeric and HMW/hexameric Acrp30 activate different signal transduction pathways, and Acrp30 represents a novel example of the control of ligand signaling via changes in its oligomerization state...
  78. pmc Disruption of reducing pathways is not essential for efficient disulfide bond formation in the cytoplasm of E. coli
    Feras Hatahet
    Department of Biochemistry, Linnanmaa campus, University of Oulu, 90570 Oulu, Finland
    Microb Cell Fact 9:67. 2010
    ..It is currently thought to be impossible to produce large amounts of disulfide bond containing protein in the cytoplasm of wild-type bacteria such as E. coli due to the presence of multiple pathways for their reduction...
  79. ncbi Crystal structures of reduced and oxidized DsbA: investigation of domain motion and thiolate stabilization
    L W Guddat
    Centre for Drug Design and Development University of Queensland Brisbane, QLD, 4072, Australia
    Structure 6:757-67. 1998
    ..In the domain motion model, hinge bending between the two domains of DsbA occurs as a result of redox-related conformational changes...
  80. ncbi Disulfide bond introduction for general stabilization of immunoglobulin heavy-chain variable domains
    Dirk Saerens
    Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Brussels, Belgium
    J Mol Biol 377:478-88. 2008
    ..Interestingly, only the extra naturally occurring cystine consistently increased the conformational and thermal stabilities of wild-type Nanobodies without affecting antigen binding...
  81. pmc Oxygen-induced maturation of SOD1: a key role for disulfide formation by the copper chaperone CCS
    Yoshiaki Furukawa
    Department of Chemistry, Northwestern University, Evanston, IL 60208, USA
    EMBO J 23:2872-81. 2004
    ..Thus Cu-CCS mediation of correct disulfide formation in SOD1 is important for regulation of enzyme activity and for prevention of misfolding or aggregation...
  82. pmc Reduction-alkylation strategies for the modification of specific monoclonal antibody disulfides
    Michael M C Sun
    Seattle Genetics, Inc, 21823 30th Drive SE, Bothell, Washington 98021, USA
    Bioconjug Chem 16:1282-90. 2005
    ..reduction and oxidation methods that lead to preferential cleavage of particular monoclonal antibody interchain disulfides using the anti-CD30 IgG1 monoclonal antibody cAC10...
  83. ncbi Snapshots of DsbA in action: detection of proteins in the process of oxidative folding
    Hiroshi Kadokura
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 303:534-7. 2004
    ..Alteration of the cis proline, highly conserved among thioredoxin superfamily members, may be useful for the detection of substrates and intermediate complexes in other systems...
  84. ncbi Redox-sensitive transcriptional control by a thiol/disulphide switch in the global regulator, Spx
    Shunji Nakano
    Department of Environmental and Biomolecular Systems, OGI School of Science and Engineering, Oregon Health and Science University, Beaverton, OR 97006, USA
    Mol Microbiol 55:498-510. 2005
    ..The mechanism of Spx-dependent transcriptional activation is unique in that it does not involve initial Spx-DNA interaction...
  85. ncbi Assignment of intrachain disulfide bonds and characterization of potential glycosylation sites of the type 1 recombinant human immunodeficiency virus envelope glycoprotein (gp120) expressed in Chinese hamster ovary cells
    C K Leonard
    Department of Medicinal Chemistry, Genentech, Inc, South San Francisco, California 94080
    J Biol Chem 265:10373-82. 1990
    ....
  86. pmc Protein modification, bioconjugation, and disulfide bridging using bromomaleimides
    Mark E B Smith
    Department of Chemistry, University College London, 20 Gordon Street, London WC1H OAJ, UK
    J Am Chem Soc 132:1960-5. 2010
    ..These results highlight the significant potential for this new class of reagents in protein modification...
  87. ncbi A thiol peroxidase is an H2O2 receptor and redox-transducer in gene activation
    Agnes Delaunay
    Laboratoire Stress Oxydants et Cancers, SBGM, DBJC, CEA Saclay, 91191 Gif sur Yvette, Cedex, France
    Cell 111:471-81. 2002
    ..These data reveal a redox-signaling function for a GPx-like enzyme and elucidate a eukaryotic hydroperoxide-sensing mechanism. Gpx3 is thus a hydroperoxide receptor and redox-transducer...
  88. pmc Redox-triggered infection by disulfide-shackled human immunodeficiency virus type 1 pseudovirions
    James M Binley
    Departments of Immunology and Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Virol 77:5678-84. 2003
    ..Overall, this disulfide-shackled virus is a unique tool with potential utility in vaccine design, drug discovery, and elucidation of the HIV-1 entry process...
  89. ncbi Impaired multimerization of human adiponectin mutants associated with diabetes. Molecular structure and multimer formation of adiponectin
    Hironori Waki
    Department of Internal Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, 113 8655, Japan
    J Biol Chem 278:40352-63. 2003
    ..In conclusion, not only total concentrations, but also multimer distribution should always be considered in the interpretation of plasma adiponectin levels in health as well as various disease states...
  90. ncbi Glutaredoxin exerts an antiapoptotic effect by regulating the redox state of Akt
    Hiroaki Murata
    Department of Biochemistry and Molecular Biology in Disease, Atomic Bomb Disease Institute, Nagasaki University School of Medicine, Japan
    J Biol Chem 278:50226-33. 2003
    ..Thus, GRX plays an important role in protecting cells from apoptosis by regulating the redox state of Akt...
  91. pmc An unusual structural motif of antimicrobial peptides containing end-to-end macrocycle and cystine-knot disulfides
    J P Tam
    Department of Microbiology and Immunology, Vanderbilt University, A 5119 MCN, 1161 21st Avenue South, Nashville, TN 37232 2363, USA
    Proc Natl Acad Sci U S A 96:8913-8. 1999
    ..Furthermore, their end-to-end cyclic structure with a cystine-knot motif represents a molecular structure of antimicrobials and may provide a useful template for the design of novel peptide antibiotics...
  92. pmc Six conserved cysteines of the membrane protein DsbD are required for the transfer of electrons from the cytoplasm to the periplasm of Escherichia coli
    E J Stewart
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    EMBO J 18:5963-71. 1999
    ..Our findings suggest a testable model for the DsbD-dependent transfer of electrons across the membrane, involving a cascade of disulfide bond reduction steps...
  93. pmc Identification of core amino acids stabilizing rhodopsin
    A J Rader
    Center for Computational Biology and Bioinformatics, Department of Molecular Biology and Biochemistry, School of Medicine, University of Pittsburgh, 200 Lothrop Street, Pittsburgh, PA 15261, USA
    Proc Natl Acad Sci U S A 101:7246-51. 2004
    ..The observed high degree of conservation (78.9%) of this disulfide bond across all GPCR classes suggests that it is critical for the stability and function of GPCRs...
  94. pmc Identification by redox proteomics of glutathionylated proteins in oxidatively stressed human T lymphocytes
    Maddalena Fratelli
    Mario Negri Institute for Pharmacological Research, 20157 Milan, Italy
    Proc Natl Acad Sci U S A 99:3505-10. 2002
    Formation of mixed disulfides between glutathione and the cysteines of some proteins (glutathionylation) has been suggested as a mechanism through which protein functions can be regulated by the redox status...
  95. ncbi A periplasmic reducing system protects single cysteine residues from oxidation
    Matthieu Depuydt
    de Duve Institute, Universite Catholique de Louvain, B 1200 Brussels, Belgium
    Science 326:1109-11. 2009
    ..DsbG interacts with the YbiS protein and, along with DsbC, regulates oxidation of its catalytic cysteine residue. Thus, a potentially widespread mechanism controls sulfenic acid modification in the cellular environment...
  96. pmc Bacterial species exhibit diversity in their mechanisms and capacity for protein disulfide bond formation
    Rachel J Dutton
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:11933-8. 2008
    ....
  97. ncbi Bioreducible BPEI-SS-PEG-cNGR polymer as a tumor targeted nonviral gene carrier
    Sejin Son
    Department of Chemistry, BK21 Program, Polymer Research Institute, Pohang University of Science and Technology POSTECH, San 31, Hyoja Dong, Nam Gu, Pohang 790 784, Republic of Korea
    Biomaterials 31:6344-54. 2010
    ....
  98. ncbi Native chemical ligation applied to the synthesis and bioengineering of circular peptides and proteins
    Richard J Clark
    Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia
    Biopolymers 94:414-22. 2010
    ..In summary, this article illustrates the use of native chemical ligation technology in the cyclization of cyclotides, sunflower trypsin inhibitor-1, and conotoxins in our laboratory...
  99. ncbi Toward a unified scheme for the aggregation of tau into Alzheimer paired helical filaments
    S Barghorn
    Max Planck Unit for Structural Molecular Biology, Notkestrasse 85, 22607 Hamburg, Germany
    Biochemistry 41:14885-96. 2002
    ..A unified view of PHF aggregation should help to analyze the causes of PHF aggregation and devise methods to prevent it...
  100. pmc Oxidative protein folding and the Quiescin-sulfhydryl oxidase family of flavoproteins
    Vamsi K Kodali
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19711, USA
    Antioxid Redox Signal 13:1217-30. 2010
    ..Finally, limitations of our current understanding of disulfide generation in metazoans are identified and questions posed for the future...
  101. ncbi Novel bioreducible poly(amido amine)s for highly efficient gene delivery
    Chao Lin
    Department of Biomedical Chemistry and Department of Polymer Chemistry and Biomaterials, Institute for Biomedical Technology BMTI, Faculty of Science and Technology, University of Twente, P O Box 217, 7500 AE Enschede, The Netherlands
    Bioconjug Chem 18:138-45. 2007
    ..The results indicate that bioreducible poly(amido amine)s have excellent properties for the development of highly potent and nontoxic polymeric gene carriers...

Research Grants62

  1. GLUTATHIONE, OXIDATIVE STRESS, AND AGING
    William C Orr; Fiscal Year: 2010
    ..of over and under expression of GCL and G6PD on the redox state of tissues indicated by GSH, GSSG, mixed protein disulfides, NADPH, NADP+, NADH and NAD+...
  2. Glutaredoxin, a Critical Regulator of Parkinson Disease Pathogenesis
    Amy L Wilson-Delfosse; Fiscal Year: 2013
    ..stress and maintains thiol homeostasis by catalyzing the reversible formation of protein-glutathione mixed disulfides on cysteine residues (protein-SSG)...
  3. Glutaredoxin, Macrophage Death and Atherosclerosis
    RETO H R ASMIS; Fiscal Year: 2010
    ..in the collapse of the glutathione/glutathione disulfide ratio, a condition that favors the formation of mixed disulfides between reactive protein thiols and glutathione in a reaction referred to as protein-S-glutathionylation...
  4. Chemokine CXCL17 and Mucosal Immunosurveillance
    Krishna Rajarathnam; Fiscal Year: 2013
    ..Both the disulfides are essential for receptor activation, and GAG binding is critical for directed migration of leukocytes...
  5. Chaetocin as a Potential Therapy for Multiple Myeloma
    Keith C Bible; Fiscal Year: 2012
    ..Mechanistically, chaetocin is rapidly and dramatically accumulated in cancer cells, requiring intact/unreduced disulfides for uptake, and imposition of ROS for cytotoxic selectivity...
  6. Conformational duality in the human chemokine Ltn/XCL1
    BRIAN VOLKMAN; Fiscal Year: 2009
    ..Ltn contains only one of the two disulfides conserved in all other chemokines, and is further distinguished by a unique disordered C-terminal extension that ..
  7. Brain aging and antioxidant supplementation
    Michael J Forster; Fiscal Year: 2010
    ..substances), (ii) shifts in glutathione redox state and amounts of aminothiols, protein sulfhydryl and mixed disulfides and (iii) levels of CoQ, vitamin E and vitamin C...
  8. TOXICITY OF CARDIAC HYPOXIA AND REOXYGENATION
    JAMES KEHRER; Fiscal Year: 1991
    ..The effect of cystamine on sarcolemmal and SR protein sulfhydryls, mixed disulfides and calcium ATPase will be assessed...
  9. EFFECT OF AGING ON INTERORGAN GLUTATHIONE HOMEOSTASIS
    Murad Ookhtens; Fiscal Year: 1999
    ..a) define the key parameters of intrahepatic and interorgan homeostasis of reduced glutathione (GSH) and thiol-disulfides; (b) define age-specific changes in them over life-span of Harlan Sprague-Dawley rats [3-5 wks (immature, I), 3-..
  10. Design, Total Synthesis &Properties of Novel Chemical Analogs of Human Insulin
    Stephen B H Kent; Fiscal Year: 2013
    ..and apply a novel total chemical synthesis of insulin that provides for highly efficient folding/formation of disulfides. Facile chemical synthesis of insulin will enable the incorporation of a wide range of non-coded amino acids in ..
  11. ALLOSTERIC ENHANCEMENT OF ADENOSINE RECEPTORS
    Joel M Linden; Fiscal Year: 2009
    ..We also have discovered that some disulfides and H2O2 have AE-like effects on the AiAR and the A^R...
  12. Inhibition of glutathione reductase and ovarian cancer drug resistance reversal
    Xiangming Guan; Fiscal Year: 2006
    ..synthetase, and glutathione S-transferase (GST), intracellular levels of GSH, GSSG, total thiols and total disulfides. Further, potential toxicity GR inhibition and a combined inhibition of GR and GCS will be investigated...
  13. PENETRATION OF MACROMOLEULCES INTO CELLS
    H Ryser; Fiscal Year: 1999
    ..Almost nothing is known about reductive cleavage of endocytosed disulfides. The key importance of this step is demonstrated by the fact that immunotoxins lose effectiveness when their ..
  14. BIOLOGY OF GLUTATHIONE PEROXIDASE IN DOPAMINE NEURONS
    Gerald Cohen; Fiscal Year: 1993
    ..provide fundamental information about the dynamics of the critical glutathione status (GSSG, GSH, protein mixed-disulfides) at dopaminergic synapses, as well as the distribution and regulation of GSH-Px and GSSG reductase in brain...
  15. PROTEIN QUALITY CONTROL IN THE ENDOPLASMIC RETICULUM
    KARL WITTRUP; Fiscal Year: 2001
    ..BPTI mutants indicate that stability, surface charge distribution, presence of free thiols, or the deletion of disulfides can each strongly influence the efficiency of escape from ER quality control...
  16. Ethyl Pyruvate: A Novel Treatment for Sepsis
    RUSSELL DELUDE; Fiscal Year: 2006
    ..Depletion of GSH shifts the cellular redox balance in a way that favors the formation of mixed disulfides with NF-KappaB subunits and thereby interferes with signaling via this pathway...
  17. OXIDANT MECHANISMS IN DRUG-INDUCED HEPATIC NECROSIS
    Charles Smith; Fiscal Year: 2006
    ..Specific Aim 1 is to test the hypothesis that specific products of oxidation of hepatic proteins other than disulfides will provide biomarkers of the molecular (chemist definition of molecular) mechanisms responsible for oxidant-..
  18. SYNTHETIC STUDIES OF PROTEIN STABILITY AND FOLDING
    George Barany; Fiscal Year: 2001
    ..disulfide bridges, and the immunoglobulin binding domain of streptococcal protein G (GB1), 56 residues without disulfides. During the first period of support, it was shown that replacement of cystine crosslinks in BPTI by paired alpha-..
  19. BIOLOGY OF MYOCARDIAL ISCHEMIA
    Mark Entman; Fiscal Year: 1990
    ..and hydroperoxides as well as the redox of various sulfhydryl compounds (glutathione, protein mixed disulfides) in cardiac lymph and myocardial biopsy samples...
  20. OPTICAL PROPERTIES OF BIOLOGICAL MACROMOLECULES
    ROBERT WOODY; Fiscal Year: 2003
    ..Improved ab initio- based parameters will be developed for side chains groups , especially disulfides. Molecular dynamic simulations will be coupled with the improved CD calculations for several small proteins...
  21. OPTICAL PROPERTIES OF BIOLOGICAL MACROMOLECULES
    ROBERT WOODY; Fiscal Year: 2002
    ..Improved ab initio- based parameters will be developed for side chains groups , especially disulfides. Molecular dynamic simulations will be coupled with the improved CD calculations for several small proteins...
  22. NHLBI MINORITY INSTITUTION FACULTY MENTORED RESEARCH
    Harpal Singh; Fiscal Year: 2001
    ..the parents compounds; b) that the hemolytic activity is associated with the formation of mixed protein-protein disulfides between hemoglobin and the skeletal proteins of the red cell; which c), appear to arise secondary to ..
  23. STRATEGIES FOR DIRECTING DISULFIDE PAIRING IN PROTEINS
    Grayson Snyder; Fiscal Year: 1993
    ..The objective of the proposed research is to develop strategies for directing formation of specific disulfides in polypeptide chains of de novo design which contain four or more cysteines...
  24. Non-disulfide protein crosslinks in human lens
    Mikhail Linetsky; Fiscal Year: 2005
    ..Except disulfides, only a few of non- disulfides lens protein crosslinks have been identified so far, all of them are acid stable, ..
  25. Functional Roles of S-S Bonding in Human Beta-Defensin 3
    Wuyuan Lu; Fiscal Year: 2004
    ..Test the hypothesis that chemotactic activity of hBD3 can be modulated by varying the topology of three disulfides. Chemotaxis indexes for these proteins will be evaluated, and the data will be correlated with binding ..
  26. BIOCHEMISTRY AND BIOPHYSICS OF BPTI FOLDING MUTANTS
    Stephen Anderson; Fiscal Year: 1993
    ..Address the problem of "designing out" disulfide bonds by making BPTI mutants missing two or more of the native disulfides; 2) Conduct a quantitative survey of all stabilizing interactions in the molecule by systematically mutating ..
  27. LAMININ PEPTIDES/RECEPTORS IN METASTATIC CELL FUNCTION
    LEO FURCHT; Fiscal Year: 1999
    ..and attempt to make various modifications by having cysteines at the amino and carboxy ends which could form disulfides between cysteines. Chemical crosslinking of amino and carboxy terminal amino acids will also be attempted...
  28. LOWER VERTEBRATE IMMUNOGLOBULIN AND B2-MICROGLOBULIN
    GARY LITMAN; Fiscal Year: 1980
    ..Identification of interchain and intrachain disulfides will be facilitated by selective reduction and radioalkylation...
  29. SULFUR-SULFUR BRIDGING IN SOLID-PHASE PEPTIDE SYNTHESIS
    George Barany; Fiscal Year: 1999
    ..The best methods are applied to achieve the syntheses of target molecules with one to three disulfides, including oxytocin, somatostatins, conotoxins, and neutrophil defensins...
  30. Redox Control of Sarcoplasmic Reticulum Calcium Release
    JONATHAN ABRAMSON; Fiscal Year: 2006
    ..to SR appears to be highly reactive sulfhydryl groups, which under mildly oxidative conditions, oxidize to disulfides. This causes the Ca2+ release channel from SR to open and the intracellular Ca2+ concentration to increase...
  31. OXIDATION CHEMISTRY OF INDOLES
    Glenn Dryhurst; Fiscal Year: 2002
    ..Specific aims are to: (1) monitor extracellular changes of DA, 5-HT and their metabolites, thiols/disulfides, and Glu, Asp and Gly in rat brain in response to MA and MDMA using microdialysis; (2) search for unusual ..
  32. OPTICAL ROTATORY POWER OF BIOPOLYMERS
    JEN YANG; Fiscal Year: 1991
    ..The role of the three intrachain disulfides in the conformation stability of the enzyme will be studied by selectively replacing the half cystines with ..
  33. FUNCTIONAL ANALYSIS OF A PROTEIN FOLDING CATALYST
    James Bardwell; Fiscal Year: 2006
    ..DsbA acts as a direct donor of disulfides to secreted proteins. It then is reoxidized by DsbB...
  34. DEVICE FOR RAPID SCREENING OF PLASMA HOMOCYSTEINE
    MALONNE DAVIES; Fiscal Year: 1999
    ..expedite the ongoing effort to better understand the role that homocysteine and other endogenous sulfhydryls and disulfides play in cardiovascular diseases, the development of a simple, reliable, high throughput and cost effective assay ..
  35. How are Disulfides transported across membranes?
    James Bardwell; Fiscal Year: 2006
    The correct formation of disulfide bonds is vital for the proper folding of proteins that possess multiple disulfides, including many proteins of pharmacological importance...
  36. COVALENT MODIFICATION OF PROTEINS BY DISULFIRAM
    LISA ZIMMERMAN; Fiscal Year: 2002
    ..Because of the structural similarities, these studies are also relevant to the biological effects of dithiocarbamates, monothiocarbamates, and dithiocarbamate disulfides used in agriculture and industry.
  37. NEW DRUGS FOR THE TREATMENT OF BREAST CANCER
    Joseph Dunn; Fiscal Year: 1999
    The objective of this project is to develop a new drug from a proprietary class of novel pyrimidine cyclic disulfides for the treatment of breast cancer...
  38. MIXED DISULFIDES AND METABOLIC CONTROL
    FRANCIS BINKLEY; Fiscal Year: 1980
    The formation of mixed disulfides in the control of metabolism in lean mice (C57B1/6J) will be contrasted with the apparent lack of such control with obese and diabetic mice (ob plus/plus ob and db plus/plus db)...
  39. MECHANISM OF FOLDING OF SERINE PROTEINASES
    ALBERT LIGHT; Fiscal Year: 1980
    ..Experimentally, the refolding of mixed disulfides of proteins and of protein fragments will be used...
  40. THIOREDOXIN IN ALL AND ITS THERAPEUTIC IMPLICATIONS
    John Yu; Fiscal Year: 2000
    ..In order to develop future therapeutic reagents for leukemia, the effect of these disulfides on leukemia colony formation as well as normal hematopoiesis in vitro will also be studied.
  41. Sulfhydryl Status in Aging Heart: Role of Glutaredoxin
    John Mieyal; Fiscal Year: 2006
    ..I/R generates reactive oxygen species (ROS) that modify thiol groups on proteins, leading to mixed disulfides between glutathione (GSH) and protein-SH (protein-SSG)...
  42. STRUCTURE OF THE HUMAN CHEMOKINE LYMPHOTACTIN
    BRIAN VOLKMAN; Fiscal Year: 2001
    ..to have unique structural features due to its 20 residue C-terminal extension and its lack of one of the two disulfides conserved in other chemokines...
  43. NEW MILD PROTECTION STRATEGIES FOR PEPTIDE SYNTHESIS
    George Barany; Fiscal Year: 1990
    ..use of the N alpha- dithiasuccinoyl (Dts) amino protecting group and the related class of open-chain carbamoyl disulfides of primary and secondary amines that were developed under this grant...
  44. KINETICS AND MECHANISMS OF PROTEIN SELF-ASSEMBLY
    DONALD WETLAUFER; Fiscal Year: 1980
    ..The time-pattern of formation of disulfides outlines a folding pathway or a manifold of such pathways...
  45. PROTEIN FOLDING AND MISFOLDING
    Michael Weiss; Fiscal Year: 2003
    ..yields a single (native) product for insulin, but yields two products (native, and one in which two of the three disulfides have swapped pairings) for IGF-1. This differential property is the basis on which Dr...
  46. STRUCTURAL STUDIES OF ACTIN
    Edward Egelman; Fiscal Year: 2006
    ..constraint in generating and interpreting such higher-resolution reconstructions will be to use engineered disulfides between adjacent subunits in the filament...
  47. PP2A-Like Constitutive ERK/MAPK Phosphatase in Brain
    Timothy Foley; Fiscal Year: 2003
    ....
  48. Metabolic analysis in human sulfur amino acid deficiency
    Dean Jones; Fiscal Year: 2005
    ..g., 300 samples/day with a flow cell), results will show whether this approach could be useful for nutritional assessment of complex metabolic effects of SAA intake. ..
  49. Metabolic effects of chemical interactions in toxicity
    Dean Jones; Fiscal Year: 2005
    ....
  50. Systematic Structural Biology of Dynein
    Stephen King; Fiscal Year: 2004
    ..These studies will provide structure-based insight into the mechanisms of dynein regulation and will provide the necessary background for further structural analysis of this massive molecular motor. ..
  51. Regulation of Celluar Zinc Distribution
    Wolfgang Maret; Fiscal Year: 2004
    ..abstract_text> ..
  52. Molecular Correlates--Oxaliplatin/5FU/XRT, Esophageal CA
    Lakshmi Pendyala; Fiscal Year: 2002
    ..The long term objectives are to identify a drug combination for better clinical outcome and to identify molecular parameters predictive for response or resistance. ..
  53. Redox Control of Radiation Response
    John Biaglow; Fiscal Year: 2006
    ..HEDS and lipoate are also used as substrates in a novel assay designed to measure cellular capacity to reduce disulfides. This assay allows us to rapidly determine which of the genetic and/or biochemical manipulations, outlined in ..
  54. Tissue Factor-factor Vlla signaling
    Usha Pendurthi; Fiscal Year: 2010
    ..The proposed studies will provide valuable information in understanding how TF contributes to tumorigenesis. These studies will also provide clues on potential therapeutic targets for prevention of cancer progression. ..
  55. Stabilized factor VIIIa as improved therapy for hemophilia A
    ANDREW GALE; Fiscal Year: 2008
    ..This treatment could improve the quality of life and overall health of the hemophilia A sufferers. ..
  56. Polyamine Catabolism in Platinum Drug Action
    Lakshmi Pendyala; Fiscal Year: 2009
    ..Overall, we expect to define mechanism-based rationale for clinical trials evaluating the combined use of Pt-drugs and DENSPM. ..
  57. REACTIVITY AND SELECTIVITY OF REACTIONS IN POLAR MEDIA
    PAUL GRIECO; Fiscal Year: 2002
    ....
  58. SOLID TUMOR SELECTIVE COMPOUNDS
    PAUL GRIECO; Fiscal Year: 2001
    ....
  59. STRUCTURE/FUNCTION OF SURFACTANT PROTEIN D
    Erika Crouch; Fiscal Year: 2006
    ..As in the past, secreted recombinant proteins with novel structural attributes will be characterized and utilized for collaborative studies of SP-D function. [unreadable] [unreadable]..