diphosphates

Summary

Summary: Inorganic salts of phosphoric acid that contain two phosphate groups.

Top Publications

  1. ncbi Nonpeptide antigens, presentation mechanisms, and immunological memory of human Vgamma2Vdelta2 T cells: discriminating friend from foe through the recognition of prenyl pyrophosphate antigens
    Craig T Morita
    Division of Rheumatology, Department of Internal Medicine, Interdisciplinary Graduate Program in Immunology, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USA
    Immunol Rev 215:59-76. 2007
  2. ncbi Plasma pyrophosphate and vascular calcification in chronic kidney disease
    W CHARLES O'NEILL
    Renal Division, Department of Medicine, Emory University, Atlanta, GA, USA
    Nephrol Dial Transplant 25:187-91. 2010
  3. ncbi The structural mechanism of translocation and helicase activity in T7 RNA polymerase
    Y Whitney Yin
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    Cell 116:393-404. 2004
  4. pmc Dynamics of pyrophosphate ion release and its coupled trigger loop motion from closed to open state in RNA polymerase II
    Lin Tai Da
    Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    J Am Chem Soc 134:2399-406. 2012
  5. pmc Active site opening and closure control translocation of multisubunit RNA polymerase
    Anssi M Malinen
    Department of Biochemistry and Food Chemistry, University of Turku, 20014, Turku, Finland
    Nucleic Acids Res 40:7442-51. 2012
  6. ncbi The history of pyrosequencing
    Pål Nyrén
    Department of Biotechnology, AlbaNova University Center, Stockholm, Sweden
    Methods Mol Biol 373:1-14. 2007
  7. ncbi In vitro expansion of gamma delta T cells with anti-myeloma cell activity by Phosphostim and IL-2 in patients with multiple myeloma
    Maka Burjanadzé
    INSERM, U847, Montpellier, France
    Br J Haematol 139:206-16. 2007
  8. ncbi Pyrosequencing: a tool for DNA sequencing analysis
    Elahe Elahi
    Stanford Genome Technology Center, Stanford University, Palo Alto, CA, USA
    Methods Mol Biol 255:211-9. 2004
  9. pmc Vgamma2Vdelta2 T Cell Receptor recognition of prenyl pyrophosphates is dependent on all CDRs
    Hong Wang
    Division of Rheumatology, Department of Internal Medicine, Interdisciplinary Graduate Program in Immunology, University of Iowa College of Medicine, Iowa City, IA 52242, USA
    J Immunol 184:6209-22. 2010
  10. pmc The nonmevalonate pathway of isoprenoid biosynthesis in Mycobacterium tuberculosis is essential and transcriptionally regulated by Dxs
    Amanda C Brown
    Queen Mary University of London, Barts and the London School of Medicine and Dentistry, London E12AD, United Kingdom
    J Bacteriol 192:2424-33. 2010

Detail Information

Publications282 found, 100 shown here

  1. ncbi Nonpeptide antigens, presentation mechanisms, and immunological memory of human Vgamma2Vdelta2 T cells: discriminating friend from foe through the recognition of prenyl pyrophosphate antigens
    Craig T Morita
    Division of Rheumatology, Department of Internal Medicine, Interdisciplinary Graduate Program in Immunology, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USA
    Immunol Rev 215:59-76. 2007
    ..Thus, primary infections with HMBPP-producing bacteria are perceived by Vgamma2Vdelta2 T cells as a repeat infection. Extensive efforts are underway to harness these cells to treat a variety of cancers and to provide microbial immunity...
  2. ncbi Plasma pyrophosphate and vascular calcification in chronic kidney disease
    W CHARLES O'NEILL
    Renal Division, Department of Medicine, Emory University, Atlanta, GA, USA
    Nephrol Dial Transplant 25:187-91. 2010
    ..We sought to determine whether plasma PPi is affected by dialysis or the mode of dialysis and whether it correlates with vascular calcification...
  3. ncbi The structural mechanism of translocation and helicase activity in T7 RNA polymerase
    Y Whitney Yin
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    Cell 116:393-404. 2004
    ..We propose that the dissociation of the product PPi after nucleotide addition produces the protein conformational change resulting in translocation and strand separation...
  4. pmc Dynamics of pyrophosphate ion release and its coupled trigger loop motion from closed to open state in RNA polymerase II
    Lin Tai Da
    Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    J Am Chem Soc 134:2399-406. 2012
    ..Our simulations provide predictions to guide future experimental tests...
  5. pmc Active site opening and closure control translocation of multisubunit RNA polymerase
    Anssi M Malinen
    Department of Biochemistry and Food Chemistry, University of Turku, 20014, Turku, Finland
    Nucleic Acids Res 40:7442-51. 2012
    ..The RNAP translocation mechanism is exploited by the antibiotic tagetitoxin, which mimics pyrophosphate and induces backward translocation by closing the active site...
  6. ncbi The history of pyrosequencing
    Pål Nyrén
    Department of Biotechnology, AlbaNova University Center, Stockholm, Sweden
    Methods Mol Biol 373:1-14. 2007
    ..I will conclude with future prospects for the method...
  7. ncbi In vitro expansion of gamma delta T cells with anti-myeloma cell activity by Phosphostim and IL-2 in patients with multiple myeloma
    Maka Burjanadzé
    INSERM, U847, Montpellier, France
    Br J Haematol 139:206-16. 2007
    ..This study demonstrated the ability of Phosphostim and IL-2 to expand gammadelta T cells from MM patients, and the efficient and stable killing of human myeloma cells by gd T cells...
  8. ncbi Pyrosequencing: a tool for DNA sequencing analysis
    Elahe Elahi
    Stanford Genome Technology Center, Stanford University, Palo Alto, CA, USA
    Methods Mol Biol 255:211-9. 2004
  9. pmc Vgamma2Vdelta2 T Cell Receptor recognition of prenyl pyrophosphates is dependent on all CDRs
    Hong Wang
    Division of Rheumatology, Department of Internal Medicine, Interdisciplinary Graduate Program in Immunology, University of Iowa College of Medicine, Iowa City, IA 52242, USA
    J Immunol 184:6209-22. 2010
    ..This underscores its parallels to innate immune receptors. Our results also provide strong evidence for the existence of an Ag-presenting molecule for prenyl pyrophosphates...
  10. pmc The nonmevalonate pathway of isoprenoid biosynthesis in Mycobacterium tuberculosis is essential and transcriptionally regulated by Dxs
    Amanda C Brown
    Queen Mary University of London, Barts and the London School of Medicine and Dentistry, London E12AD, United Kingdom
    J Bacteriol 192:2424-33. 2010
    ..Increased expression of this operon was seen in cells overexpressing Dxs1, indicating that transcriptional control is effected by the first enzyme of the pathway via an unknown regulator...
  11. ncbi Aminobisphosphonate-pretreated dendritic cells trigger successful Vgamma9Vdelta2 T cell amplification for immunotherapy in advanced cancer patients
    Florian Cabillic
    EE341 Biothérapies Innovantes, Faculte de Medecine, Universite de Rennes 1, Rennes, France
    Cancer Immunol Immunother 59:1611-9. 2010
    ..Thus, this method of generating Vgamma9Vdelta2 T cells leads eligible for Vgamma9Vdelta2 T cell adoptive immunotherapy the HCC and mCRC patients...
  12. ncbi Microbial isoprenoid biosynthesis and human gammadelta T cell activation
    Matthias Eberl
    Biochemisches Institut, Justus Liebig Universitat Giessen, Friedrichstr 24, Germany
    FEBS Lett 544:4-10. 2003
    ..Unexpectedly, the intermediate of the MEP pathway, (E)-4-hydroxy-3-methyl-but-2-enyl-pyrophosphate) (HMB-PP), turned out to be by far the most potent Vgamma9/Vdelta2 T cell activator known, with an EC(50) of 0.1 nM...
  13. ncbi Reduced plasma pyrophosphate levels in hemodialysis patients
    Koba A Lomashvili
    Emory University, Renal Division, WMB 338, 1639 Pierce Drive, Atlanta, GA 30322, USA
    J Am Soc Nephrol 16:2495-500. 2005
    ..Plasma levels in some patients were below those that have previously been shown to prevent calcification of vessels in culture, suggesting that altered PPi metabolism could contribute to vascular calcification in hemodialysis patients...
  14. ncbi Up-regulated expression of cartilage intermediate-layer protein and ANK in articular hyaline cartilage from patients with calcium pyrophosphate dihydrate crystal deposition disease
    Jun Hirose
    Medical College of Wisconsin, Milwaukee
    Arthritis Rheum 46:3218-29. 2002
    ....
  15. pmc PC-1 nucleoside triphosphate pyrophosphohydrolase deficiency in idiopathic infantile arterial calcification
    F Rutsch
    Department of Pediatrics, Municipal Children s Hospital, Dortmund, Germany
    Am J Pathol 158:543-54. 2001
    ....
  16. pmc The mechanism of the translocation step in DNA replication by DNA polymerase I: a computer simulation analysis
    Andrei A Golosov
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
    Structure 18:83-93. 2010
    ..These roles are consistent with the high level of conservation of Y714 and the two glycine residues at these positions. It is likely that a corresponding mechanism is applicable to other polymerases...
  17. ncbi Linked deficiencies in extracellular PP(i) and osteopontin mediate pathologic calcification associated with defective PC-1 and ANK expression
    Kristen Johnson
    Veterans Affairs Medical Center, University of California San Diego, La Jolla, California 92161, USA
    J Bone Miner Res 18:994-1004. 2003
    ..Thus, linked regulatory effects on extracellular PP(i) and OPN expression mediate the ability of PC-1 and ANK to regulate calcification...
  18. pmc Support of a free radical mechanism for enhanced antitumor efficacy of the microtubule disruptor OXi4503
    Lori Rice
    Department of Radiation Oncology, University of Florida, Gainesville, FL 32610, USA
    Microvasc Res 81:44-51. 2011
    ..These findings indicate that while the predominant in vivo effect of OXi4503 is clearly due to microtubule collapse and vascular shut-down, the formation of toxic free radicals likely contributes to its enhanced potency...
  19. ncbi Role of the progressive ankylosis gene in cartilage mineralization
    Raihana Zaka
    Department of Medicine Division of Rheumatology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Curr Opin Rheumatol 18:181-6. 2006
    ..The purpose of this review is to highlight recent work on the role of ANK in physiological and pathological calcification of articular and growth plate cartilage...
  20. ncbi Chondrogenesis mediated by PPi depletion promotes spontaneous aortic calcification in NPP1-/- mice
    Kristen Johnson
    Rheumatology Medicine, Veterans Affairs Medical Center University of California at San Diego, School of Medicine, CA 92161, USA
    Arterioscler Thromb Vasc Biol 25:686-91. 2005
    ....
  21. pmc Inhibition of phosphoantigen-mediated gammadelta T-cell proliferation by CD4+ CD25+ FoxP3+ regulatory T cells
    Volker Kunzmann
    University of Wurzburg, Medizinische Klinik und Poliklinik II, Wurzburg, Germany
    Immunology 126:256-67. 2009
    ....
  22. pmc High throughput automated allele frequency estimation by pyrosequencing
    Julie Doostzadeh
    The Parkinson s Institute, Sunnyvale, California, United States of America
    PLoS ONE 3:e2693. 2008
    ..We also discuss potential improvements of the software for more accurate allele frequency analysis...
  23. ncbi Engineering of elongation complexes of bacterial and yeast RNA polymerases
    Natalia Komissarova
    NCI Center for Cancer Research, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, Maryland 21702, USA
    Methods Enzymol 371:233-51. 2003
  24. ncbi Subcellular pyrophosphate metabolism in developing tubers of potato (Solanum tuberosum)
    Eva M Farré
    Max Planck Institut fur Molekulare Pflanzenphysiologie, Am Muhlenberg 1, 14476 Golm, Germany
    Plant Mol Biol 62:165-79. 2006
    ..These results are discussed both with respect to our previous hypotheses and to current models of the compartmentation of potato tuber metabolism...
  25. ncbi A continuous fluorescent assay for protein prenyltransferases measuring diphosphate release
    June E Pais
    Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Anal Biochem 345:302-11. 2005
    ..This assay can be adapted for high-throughput screening for potential prenyltransferase substrates and inhibitors...
  26. ncbi Phase I study of bromohydrin pyrophosphate (BrHPP, IPH 1101), a Vgamma9Vdelta2 T lymphocyte agonist in patients with solid tumors
    Jaafar Bennouna
    Department of Medical Oncology, Centre Rene Gauducheau, 44805, Nantes Saint Herblain, France
    Cancer Immunol Immunother 59:1521-30. 2010
    ..The present phase I trial was conducted with the aim of determining the maximum-tolerated dose (MTD) and safety of IPH1101 combined with a low dose of IL-2 in patients with solid tumors...
  27. ncbi Phosphorylation of pyrimidine deoxynucleoside analog diphosphates: selective phosphorylation of L-nucleoside analog diphosphates by 3-phosphoglycerate kinase
    Preethi Krishnan
    Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 277:5453-9. 2002
    ..Metabolism of L-nucleoside analog diphosphates to the triphosphates, however, remains unexplored...
  28. pmc Loss of skeletal mineralization by the simultaneous ablation of PHOSPHO1 and alkaline phosphatase function: a unified model of the mechanisms of initiation of skeletal calcification
    Manisha C Yadav
    Sanford Children s Health Research Center, Sanford Burnham Medical Research Institute, La Jolla, CA 92037, USA
    J Bone Miner Res 26:286-97. 2011
    ....
  29. ncbi Overexpression of a cytosolic pyrophosphatase (TgPPase) reveals a regulatory role of PP(i) in glycolysis for Toxoplasma gondii
    Douglas A Pace
    Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA
    Biochem J 440:229-40. 2011
    ..These results implicate PP(i) as having a significant regulatory role in glycolysis and, potentially, other downstream processes that regulate growth and cell division...
  30. ncbi Role of the mouse ank gene in control of tissue calcification and arthritis
    A M Ho
    Department of Developmental Biology and Howard Hughes Medical Institute, Beckman Center B300, Stanford University School of Medicine, Stanford, CA 94305 5327, USA
    Science 289:265-70. 2000
    ..These results identify ANK-mediated control of pyrophosphate levels as a possible mechanism regulating tissue calcification and susceptibility to arthritis in higher animals...
  31. ncbi Inorganic pyrophosphate generation and disposition in pathophysiology
    R A Terkeltaub
    Veterans Affairs Medical Center and School of Medicine, University of California, San Diego, California 92161, USA
    Am J Physiol Cell Physiol 281:C1-C11. 2001
    ....
  32. pmc In vivo functional differences in microvascular response of 4T1 and Caki-1 tumors after treatment with OXi4503
    Mamta Wankhede
    J Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL 32611, USA
    Oncol Rep 23:685-92. 2010
    ..The differential responses of these two tumor-types suggest that further investigation of the mechanisms of action of VDAs and individual characterization of tumor VDA-responses may be needed for optimal clinical use of these agents...
  33. ncbi Methanococcus jannaschii ORF mj0608 codes for a class C inorganic pyrophosphatase protected by Co(2+) or Mn(2+) ions against fluoride inhibition
    N J Kuhn
    School of Biosciences, The University of Birmingham, Birmingham, B15 2TT, United Kingdom
    Arch Biochem Biophys 379:292-8. 2000
    ..However, prior exposure to these ions afforded striking protection against inhibition by sodium fluoride, to which the enzyme was otherwise very sensitive...
  34. pmc Correction of hypophosphatasia-associated mineralization deficiencies in vitro by phosphate/pyrophosphate modulation in periodontal ligament cells
    Thaisângela L Rodrigues
    Department of Prosthodontics and Periodontics, Campinas State University, Piracicaba, Sao Paulo, Brazil
    J Periodontol 83:653-63. 2012
    ....
  35. ncbi Vascular targeting agent Oxi4503 inhibits tumor growth in a colorectal liver metastases model
    Caterina Malcontenti-Wilson
    Department of Surgery, Austin Hospital, University of Melbourne, Melbourne, Victoria, Australia
    J Gastroenterol Hepatol 23:e96-e104. 2008
    ..Despite evidence of its efficacy in certain malignancies, the effect on colorectal liver metastases remains largely unknown. This study investigates the effect of Oxi4503 on colorectal liver metastases in a murine model...
  36. ncbi Phase I clinical and pharmacokinetic evaluation of the vascular-disrupting agent OXi4503 in patients with advanced solid tumors
    Dan M Patterson
    Mount Vernon Cancer Centre, Mount Vernon Hospital, Northwood, Middlesex, UK
    Clin Cancer Res 18:1415-25. 2012
    ..Preclinical studies show that OXi4503 (combretastatin A1 diphosphate, CA1P) is more potent than other clinically evaluated vascular-disrupting agents...
  37. ncbi Molecular determinants of extracellular matrix mineralization in bone and blood vessels
    Monzur Murshed
    Department of Medicine, McGill University, Montreal, Quebec, Canada
    Curr Opin Nephrol Hypertens 19:359-65. 2010
    ..Here we review new findings on direct molecular determinants of mineralization operating locally at the level of the extracellular matrix, with a focus on bone and blood vessels...
  38. ncbi Alterations in vascular architecture and permeability following OXi4503 treatment
    Lie Sam Chan
    Department of Surgery, University of Melbourne, Austin Health, Heidelberg, Victoria, Australia
    Anticancer Drugs 19:17-22. 2008
    ..A combination of OXi4503 with other chemotherapeutic modalities might achieve complete tumor eradication and improve long-term survival...
  39. pmc A neutral diphosphate mimic crosslinks the active site of human O-GlcNAc transferase
    Jiaoyang Jiang
    Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, USA
    Nat Chem Biol 8:72-7. 2012
    ..Though this unprecedented double-displacement mechanism reflects the unique architecture of the OGT active site, related dicarbamate scaffolds may inhibit other enzymes that bind nucleotide-containing substrates...
  40. ncbi Combretastatin family member OXI4503 induces tumor vascular collapse through the induction of endothelial apoptosis
    Yezhou Sheng
    Department of Cell and Molecular Biology, Section for Tumor Immunology, University of Lund, Lund, Sweden
    Int J Cancer 111:604-10. 2004
    ..The time course of the tumor vascular response observed with OXI4503 treatment supports this drug for development as a stand alone therapy, and also lends support for the use of the drug in combination with other cancer therapies...
  41. pmc Identification of the inorganic pyrophosphate metabolizing, ATP substituting pathway in mammalian spermatozoa
    Young Joo Yi
    Division of Animal Sciences, University of Missouri Columbia, Columbia, Missouri, United States of America
    PLoS ONE 7:e34524. 2012
    ....
  42. ncbi Cementum: a phosphate-sensitive tissue
    F H Nociti
    Department of Periodontics Prevention Geriatrics, School of Dentistry, Rm 3310M Dental, University of Michigan, 1011 N University Avenue, Ann Arbor, MI 48109 1078, USA
    J Dent Res 81:817-21. 2002
    ..This information may prove valuable in attempts to design successful therapies for regenerating periodontal tissues...
  43. ncbi Pyrosequencing sheds light on DNA sequencing
    M Ronaghi
    Genome Technology Center, Stanford University, Palo Alto, California 94304, USA
    Genome Res 11:3-11. 2001
    ..This article considers key features regarding different aspects of pyrosequencing technology, including the general principles, enzyme properties, sequencing modes, instrumentation, and potential applications...
  44. ncbi Effect of vascular targeting agent Oxi4503 on tumor cell kinetics in a mouse model of colorectal liver metastasis
    Lie Sam Chan
    University of Melbourne, Department of Surgery, Austin Hospital, Heidelberg, Victoria 3084, Australia
    Anticancer Res 27:2317-23. 2007
    ..This agent appears to selectively target the endothelial cytoskeleton with resultant vessel occlusion and tumor necrosis...
  45. ncbi Human gamma delta T cells induce dendritic cell maturation
    Jamila Ismaili
    Laboratoire d Immunologie Expérimentale, Universite Libre de Bruxelles, Brussels, Belgium
    Clin Immunol 103:296-302. 2002
    ..We conclude that gamma delta T cell activation might result in DC maturation and thereby in enhanced alpha beta T cell responses...
  46. pmc Unique coexpression in osteoblasts of broadly expressed genes accounts for the spatial restriction of ECM mineralization to bone
    Monzur Murshed
    Department of Molecular and Human Genetics, Bone Disease Program of Texas, Baylor College of Medicine, Houston, TX 77030, USA
    Genes Dev 19:1093-104. 2005
    ....
  47. pmc Tissue-nonspecific alkaline phosphatase and plasma cell membrane glycoprotein-1 are central antagonistic regulators of bone mineralization
    Lovisa Hessle
    The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 99:9445-9. 2002
    ..Our results suggest that inhibiting PC-1 function may be a viable therapeutic strategy for hypophosphatasia. Conversely, interfering with TNAP activity may correct pathological hyperossification because of PP(i) insufficiency...
  48. pmc A rapid crosstalk of human gammadelta T cells and monocytes drives the acute inflammation in bacterial infections
    Matthias Eberl
    Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Cardiff, UK
    PLoS Pathog 5:e1000308. 2009
    ....
  49. ncbi Drug-induced expansion and differentiation of V gamma 9V delta 2 T cells in vivo: the role of exogenous IL-2
    Rita Casetti
    National Institute for Infectious Diseases, Lazzaro Spallanzani, Istituto di Ricerca e Cura a Carattere Scientifico, Rome, Italy
    J Immunol 175:1593-8. 2005
    ..These results may be relevant for designing novel (or modifying current) immunotherapeutic trials with nitrogen-containing bisphosphonate or pyrophosphomonoester drugs...
  50. ncbi Pyrophosphate inhibits mineralization of osteoblast cultures by binding to mineral, up-regulating osteopontin, and inhibiting alkaline phosphatase activity
    William N Addison
    Faculty of Dentistry, McGill University, Montreal, Quebec H3A 2B2, Canada
    J Biol Chem 282:15872-83. 2007
    ..In summary, PP(i) prevents mineralization in MC3T3-E1 osteoblast cultures by at least three different mechanisms that include direct binding to growing crystals, induction of Opn expression, and inhibition of Tnap activity...
  51. pmc Poliovirus RNA-dependent RNA polymerase (3Dpol): pre-steady-state kinetic analysis of ribonucleotide incorporation in the presence of Mg2+
    Jamie J Arnold
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Biochemistry 43:5126-37. 2004
    ..We discuss the implications of this work on the development of RNA-dependent RNA polymerase inhibitors for use as antiviral agents...
  52. ncbi Monocytes and gammadelta T cells: close encounters in microbial infection
    Matthias Eberl
    Department of Infection, Immunity and Biochemistry, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, United Kingdom
    Trends Immunol 30:562-8. 2009
    ..Vgamma9/Vdelta2 T cells act thus as a cellular switch between innate and adaptive defence mechanisms...
  53. pmc Multiplex SNP typing by bioluminometric assay coupled with terminator incorporation (BATI)
    Guo Hua Zhou
    Central Research Laboratory, Hitachi, Ltd 1 280 Higashi Koigakubo, Kokubunji shi, Tokyo 185 8601, Japan
    Nucleic Acids Res 33:e133. 2005
    ..These results indicate that this novel multiplexed approach can significantly decrease the cost of SNP typing and increase the typing throughput with an inexpensive and small luminometer...
  54. pmc State-dependent modulation of CFTR gating by pyrophosphate
    Ming Feng Tsai
    Department of Medical Pharmacology and Physiology, University of Missouri Columbia, Missouri 65211, USA
    J Gen Physiol 133:405-19. 2009
    ..Finally, we show that MgAMP-PNP exerts its effects on CFTR gating via a similar mechanism as MgPPi. The structural and functional significance of our findings is discussed...
  55. pmc Role of the progressive ankylosis gene (ank) in cartilage mineralization
    Wei Wang
    Department of Orthopaedics, University of Maryland School of Medicine, 22 South Greene Street, Baltimore, MD 21201, USA
    Mol Cell Biol 25:312-23. 2005
    ....
  56. ncbi Preclinical evaluation of vascular-disrupting agents in Ewing's sarcoma family of tumours
    Surita Dalal
    Candlelighter s Children s Cancer Research Group, Cancer Research UK Clinical Centre, Leeds Institute of Molecular Medicine, St James s University Hospital, Beckett Street, Leeds, West Yorkshire LS9 7TF, UK
    Eur J Cancer 45:713-22. 2009
    ..001). This study demonstrates that OXi4503/CA1P is a potent VDA in ESFT and in combination with conventional cytotoxic agents represents a promising treatment strategy for this tumour group...
  57. pmc Phosphoantigen presentation by macrophages to mycobacterium tuberculosis--reactive Vgamma9Vdelta2+ T cells: modulation by chloroquine
    Roxana E Rojas
    Department of Medicine, Case Western Reserve University and University Hospitals of Cleveland, Ohio 44106, USA
    Infect Immun 70:4019-27. 2002
    ..Chloroquine, by inhibiting membrane traffic, may increase association and retention of phosphoantigens with cell surface membrane molecules on macrophages...
  58. pmc Characterization of an archaeal medium-chain acyl coenzyme A synthetase from Methanosarcina acetivorans
    Yu Meng
    Clemson University, Department of Genetics and Biochemistry, Clemson, SC 29634 0318, USA
    J Bacteriol 192:5982-90. 2010
    ..Characterization of the enzyme variants indicates that these six residues are critical in acyl substrate binding and catalysis, and even conservative alterations significantly reduced the catalytic ability of the enzyme...
  59. ncbi Cementum and dentin in hypophosphatasia
    T Van den Bos
    Department of Periodontology, Academic Center for Dentistry Amsterdam ACTA, Universiteit van Amsterdam, and Vrije Universiteit, Louwesweg 1, 1066 EA Amsterdam, The Netherlands
    J Dent Res 84:1021-5. 2005
    ..Our findings suggest that mineralization of dentin is less likely to be under the influence of the inhibitory action of pyrophosphate than mineralization of cementum...
  60. ncbi Differential mechanisms of inorganic pyrophosphate production by plasma cell membrane glycoprotein-1 and B10 in chondrocytes
    K Johnson
    Department of Veterans Affairs Medical Center, University of California, San Diego 92161, USA
    Arthritis Rheum 42:1986-97. 1999
    ..This study compared the roles of the transforming growth factor beta (TGFbeta)-inducible plasma cell membrane glycoprotein-1 (PC-1) and the closely related B10 NTPPPH activities in chondrocyte PPi metabolism...
  61. pmc Concerted regulation of inorganic pyrophosphate and osteopontin by akp2, enpp1, and ank: an integrated model of the pathogenesis of mineralization disorders
    Dympna Harmey
    The Burnham Institute, La Jolla, CA 92037, USA
    Am J Pathol 164:1199-209. 2004
    ..The hypomineralization observed in Akp2(-/-) mice arises from the combined inhibitory effects of PP(i) and OPN. In contrast, NPP1 or ANK deficiencies cause a decrease in the PP(i) and OPN pools that leads to hypermineralization...
  62. ncbi Short-chain phosphatidates are subtype-selective antagonists of lysophosphatidic acid receptors
    D J Fischer
    Department of Physiology, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA
    Mol Pharmacol 60:776-84. 2001
    ....
  63. ncbi Excision of incorporated nucleotide analogue chain-terminators can diminish their inhibitory effects on viral RNA-dependent RNA polymerases
    Claudia M D'Abramo
    McGill University AIDS Centre, Lady Davis Institute Jewish General Hospital, Montreal, Que, Canada
    J Mol Biol 337:1-14. 2004
    ....
  64. ncbi NUDT5 hydrolyzes oxidized deoxyribonucleoside diphosphates with broad substrate specificity
    Hiroyuki Kamiya
    Hokkaido University, Sapporo, Japan
    DNA Repair (Amst) 8:1250-4. 2009
    ..These results suggest that the NUDT5 protein eliminates various oxidized deoxyribonucleoside diphosphates from the nucleotide pool and prevents their toxic effects.
  65. pmc Pyrophosphorolysis-activatable oligonucleotides may facilitate detection of rare alleles, mutation scanning and analysis of chromatin structures
    Qiang Liu
    Departments of Molecular Genetics, City of Hope National Medical Center, 1500 East Duarte Road, Duarte, CA 91010 3000, USA
    Nucleic Acids Res 30:598-604. 2002
    ..Thus, PAP constitutes a technology platform of potential utility whenever high specificity is required along the length of an oligonucleotide...
  66. ncbi Quantification of mtDNA mixtures in forensic evidence material using pyrosequencing
    H Andreasson
    Rudbeck Laboratory, Department of Genetics and Pathology, Uppsala University, 75185 Uppsala, Sweden
    Int J Legal Med 120:383-90. 2006
    ..This mtDNA quantification assay is an easy to use, fast and accurate quantification system, with the ability to resolve and interpret major and minor mtDNA components in forensic mixture samples...
  67. ncbi Cloning and characterization of two different types of geranyl diphosphate synthases from Norway spruce (Picea abies)
    Axel Schmidt
    Max Planck Institute for Chemical Ecology, Department of Biochemistry, Hans Knoll Strasse 8, D 07745 Jena, Germany
    Phytochemistry 69:49-57. 2008
    ..and one encoded by a similar gene sequence from Quercus robur gave substantial amounts of the larger prenyl diphosphates, FPP and GGPP, in addition to GPP...
  68. ncbi Synthesis and biological activity of phosphonate analogues and geometric isomers of the highly potent phosphoantigen (E)-1-hydroxy-2-methylbut-2-enyl 4-diphosphate
    Angélique Boëdec
    Innate Pharma, 121 Ancien Chemin de Cassis, Marseille, France
    J Med Chem 51:1747-54. 2008
    ..These results indicate that the use of bioisosteric analogues of HDMAPP may represent promising new leads for immunotherapy...
  69. ncbi Rapid kinetic analysis of transcription elongation by Escherichia coli RNA polymerase
    Ronald S Johnson
    Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina University, Greenville, NC 27834, USA
    J Mol Biol 381:1106-13. 2008
    ..Rapid release of pyrophosphate from the elongation complex at a rate consistent with productive transcription elongation occurs only in the presence of the correct next nucleotide for incorporation into the transcript...
  70. pmc Kinetic analysis of substrate utilization by native and TNAP-, NPP1-, or PHOSPHO1-deficient matrix vesicles
    Pietro Ciancaglini
    Sanford Children s Health Research Center, Sanford Burnham Medical Research Institute, La Jolla, CA, USA
    J Bone Miner Res 25:716-23. 2010
    ..NPP1 does not have a major role in PP(i) generation from ATP at the level of MVs, in contrast to its accepted role on the surface of the osteoblasts and chondrocytes, but rather acts as a phosphatase in the absence of TNAP...
  71. pmc Regulation of vascular smooth muscle cell calcification by extracellular pyrophosphate homeostasis: synergistic modulation by cyclic AMP and hyperphosphatemia
    Domenick A Prosdocimo
    Dept of Physiology and Biophysics, Case Western Reserve Univ, School of Medicine, 2109 Adelbert Rd, Cleveland, OH 44106, USA
    Am J Physiol Cell Physiol 298:C702-13. 2010
    ..These observations support a model in which extracellular PP(i) generation mediated by both ANK- and ATP release-dependent mechanisms serves as a critical regulator of VSMC calcification...
  72. ncbi Oxi4503, a novel vascular targeting agent: effects on blood flow and antitumor activity in comparison to combretastatin A-4 phosphate
    Jianyi Hua
    Department of Cell and Molecular Biology, Section for Tumor Immunology, University of Lund, BMC 112, 22184 Lund, Sweden
    Anticancer Res 23:1433-40. 2003
    ..These results are promising for the use of Oxi4503 as a tumor vascular targeting agent. Moreover the potent antitumor effect when administered as a single agent suggests a different activity profile than CA4P...
  73. ncbi Preferential recognition of a microbial metabolite by human Vgamma2Vdelta2 T cells
    Kia Joo Puan
    Division of Rheumatology, Department of Internal Medicine and the Interdisciplinary Graduate Program in Immunology, University of Iowa College of Medicine, EMRB 400F, Iowa City, IA 52242, USA
    Int Immunol 19:657-73. 2007
    ..This preferential stimulation by a common microbial isoprenoid metabolite allows Vgamma2Vdelta2 T cells to respond to a broad array of pathogens using this pathway...
  74. ncbi Differential sensitivity of the cystic fibrosis (CF)-associated mutants G551D and G1349D to potentiators of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel
    Zhiwei Cai
    Department of Physiology, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, United Kingdom
    J Biol Chem 281:1970-7. 2006
    ..We conclude that G551D- and G1349D-CFTR have distinct pharmacological profiles and speculate that drug therapy for CF is likely to be mutation-specific...
  75. pmc Differential regulation of soluble and membrane-bound inorganic pyrophosphatases in the photosynthetic bacterium Rhodospirillum rubrum provides insights into pyrophosphate-based stress bioenergetics
    Rosa L Lopez-Marques
    Instituto de Bioquimica Vegetal y Fotosintesis, Universidad de Sevilla CSIC, 41092 Seville, Spain
    J Bacteriol 186:5418-26. 2004
    ..These results demonstrate a tight transcriptional regulation of the H(+)-PPase gene, which appears to be induced in response to a variety of environmental conditions, all of which constrain cell energetics...
  76. ncbi Potentiation of antigen-stimulated V gamma 9V delta 2 T cell cytokine production by immature dendritic cells (DC) and reciprocal effect on DC maturation
    Marie Claire Devilder
    Institut National de la Sante et de la Recherche Medicale, Unite 601, Nantes, France
    J Immunol 176:1386-93. 2006
    ....
  77. ncbi Upregulated ank expression in osteoarthritis can promote both chondrocyte MMP-13 expression and calcification via chondrocyte extracellular PPi excess
    K Johnson
    Veterans Affairs Medical Center, UCSD, La Jolla, CA 92161, USA
    Osteoarthritis Cartilage 12:321-35. 2004
    ..Therefore, we assessed if dysregulated wild-type ANK expression could contribute to pathogenesis of idiopathic degenerative arthropathy through elevated ecPP(i)...
  78. ncbi PAP: detection of ultra rare mutations depends on P* oligonucleotides: "sleeping beauties" awakened by the kiss of pyrophosphorolysis
    Qiang Liu
    Department of Molecular Genetics, City of Hope National Medical Center, Duarte, California 91010 3000, USA
    Hum Mutat 23:426-36. 2004
    ..Thus, PAP also can form the basis of microarray-based scanning or resequencing methods to detect virtually all mutations...
  79. ncbi The interplay between classical and alternative isoprenoid biosynthesis controls gammadelta T cell bioactivity of Listeria monocytogenes
    Maire Begley
    Department of Microbiology and Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland
    FEBS Lett 561:99-104. 2004
    ..Vgamma9/Vdelta2 T cell bioactivity is increased in DeltalytB mutants where HMB-PP accumulation is expected, and lost in DeltagcpE mutants which fail to produce HMB-PP...
  80. pmc Relationship between 3'-azido-3'-deoxythymidine resistance and primer unblocking activity in foscarnet-resistant mutants of human immunodeficiency virus type 1 reverse transcriptase
    Peter R Meyer
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Florida 33101, USA
    J Virol 77:6127-37. 2003
    ....
  81. ncbi Pyrosequencing-based SNP allele frequency estimation in DNA pools
    Catharina Lavebratt
    Department of Molecular Medicine, Karolinska Institutet, Karolinska Hospital, Stockholm, Sweden
    Hum Mutat 23:92-7. 2004
    ..Considering the ease of use, short run and analysis times, and little instrument maintenance requirements, Pyrosequencing may even be a preferred option...
  82. ncbi Association of sporadic chondrocalcinosis with a -4-basepair G-to-A transition in the 5'-untranslated region of ANKH that promotes enhanced expression of ANKH protein and excess generation of extracellular inorganic pyrophosphate
    Yun Zhang
    Botnar Research Centre, Nuffield Orthopaedic Centre, University of Oxford, Oxford, UK
    Arthritis Rheum 52:1110-7. 2005
    ..This study investigated the potential for ANKH sequence variants to promote sporadic chondrocalcinosis...
  83. pmc Intracellular substrates for the primer-unblocking reaction by human immunodeficiency virus type 1 reverse transcriptase: detection and quantitation in extracts from quiescent- and activated-lymphocyte subpopulations
    Anthony J Smith
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, P O Box 016129, Miami, FL 33101 6129, USA
    Antimicrob Agents Chemother 49:1761-9. 2005
    ..While PP(i)-dependent excision may contribute to AZT resistance in vivo, it is likely that selection of AZT-resistant mutants occurs primarily in an environment where the ATP-dependent reaction predominates...
  84. pmc Functional complementation of yeast cytosolic pyrophosphatase by bacterial and plant H+-translocating pyrophosphatases
    Jose R Perez-Castineira
    Instituto de Bioquimica Vegetal y Fotosintesis Universidad de Sevilla Consejo Superior de Investigaciones Cientificas, Avda Américo Vespucio sn, 41092 Sevilla, Spain
    Proc Natl Acad Sci U S A 99:15914-9. 2002
    ..YPC-1 mutant strain constitutes a convenient expression system to perform studies aimed at the elucidation of the structure-function relationships of this type of proton pumps...
  85. ncbi Differentiation of human gamma-delta T cells towards distinct memory phenotypes
    Matthias Eberl
    Biochemisches Institut, Justus Liebig Universitat Giessen, Friedrichstr 24, 35392 Giessen, Germany
    Cell Immunol 218:1-6. 2002
    ..In contrast, Vgamma9/Vdelta2 T cells grown with IL-21 differentiated into putative central memory CD45RO(+) T cells that did not produce TNF-alpha, IFN-gamma, or IL-4, and maintained expression of CD62L, CD81, and CCR7...
  86. ncbi Replacing the pyrophosphate group of HMB-PP by a diphosphonate function abrogates Its potential to activate human gammadelta T cells but does not lead to competitive antagonism
    Armin Reichenberg
    Jomaa Pharmaka GmbH, Frankfurter Str 50, D 35392 Giessen, Germany
    Bioorg Med Chem Lett 13:1257-60. 2003
    ..However, replacing the pyrophosphate by a P-CH(2)-P function abrogates the bioactivity drastically, with HMB-PCP having a EC(50) of only 5.3 microM...
  87. ncbi Multidrug-resistant HIV-1 reverse transcriptase: involvement of ribonucleotide-dependent phosphorolysis in cross-resistance to nucleoside analogue inhibitors
    Antonio Mas
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas Universidad Autonoma de Madrid, Cantoblanco, 28049, Madrid, Spain
    J Mol Biol 323:181-97. 2002
    ..This proposal could explain why insertion-containing HIV-1 variants have been detected in the absence of AZT, during d4T treatment...
  88. pmc Distinct cytokine-driven responses of activated blood gammadelta T cells: insights into unconventional T cell pleiotropy
    David Vermijlen
    Peter Gorer Department of Immunobiology, Guy s, King s and St Thomas Medical School, King s College London, London, UK
    J Immunol 178:4304-14. 2007
    ....
  89. pmc A possible role for pyrophosphate in the coordination of cytosolic and plastidial carbon metabolism within the potato tuber
    E M Farré
    Max Planck Institut fur Molekulare Pflanzenphysiologie, Golm, Germany
    Plant Physiol 123:681-8. 2000
    ....
  90. ncbi Bioluminometric method for real-time detection of reverse transcriptase activity
    S Karamohamed
    Royal Institute of Technology, Stockholm, Sweden
    Biotechniques 24:302-6. 1998
    ..The possibility of using the new technique for other applications is discussed...
  91. ncbi Cleavage of 3',5'-pyrophosphate-linked dinucleotides by ribonuclease A and angiogenin
    A M Jardine
    Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
    Biochemistry 40:10262-72. 2001
    ....
  92. pmc Quantitative detection of single nucleotide polymorphisms for a pooled sample by a bioluminometric assay coupled with modified primer extension reactions (BAMPER)
    G Zhou
    Hitachi Ltd, Central Research Laboratory, 1-280 Higashi-Koigakubo, Kokubunji-shi, Tokyo 185-8601, Japan
    Nucleic Acids Res 29:E93. 2001
    ..The method is sensitive enough to detect 14 amol ssM13 DNA. The proposed method seems very promising in terms of realizing a cost-effective, large-scale human genetic testing system...
  93. pmc Efficient pyrophosphorolysis by a hepatitis B virus polymerase may be a primer-unblocking mechanism
    S Urban
    Department of Medical Microbiology and Immunology and Glaxo Wellcome Heritage Research Institute, University of Alberta, Edmonton, AB, Canada, T6G 2H7
    Proc Natl Acad Sci U S A 98:4984-9. 2001
    ..The strong inhibitory effect of cytoplasmic pyrophosphate concentrations on viral DNA synthesis may also partly account for the apparent slow rate of HBV genome replication...
  94. pmc Chimeric constructs endow the human CFTR Cl- channel with the gating behavior of murine CFTR
    Toby S Scott-Ward
    Department of Physiology and Pharmacology, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, United Kingdom
    Proc Natl Acad Sci U S A 104:16365-70. 2007
    ..We conclude that the NBDs of murine CFTR determine the unique gating behavior of its subconductance state, whereas NBD2 controls channel potentiation by PP(i)...
  95. pmc Prolonged (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate-driven antimicrobial and cytotoxic responses of pulmonary and systemic Vgamma2Vdelta2 T cells in macaques
    Zahida Ali
    Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois, College of Medicine, Chicago, IL 60612, USA
    J Immunol 179:8287-96. 2007
    ..Thus, the prolonged HMBPP-driven antimicrobial and cytotoxic responses of pulmonary and systemic Vgamma2Vdelta2 T cells may confer immunotherapeutics against infectious diseases and cancers...
  96. ncbi Identification of (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate as a major activator for human gammadelta T cells in Escherichia coli
    M Hintz
    Jomaa Pharmaka GmbH, Frankfurter Strasse 50, 35392 Giessen, Germany
    FEBS Lett 509:317-22. 2001
    ..HMB-PP is 10(4) times more potent in activating human Vgamma9/Vdelta2 T cells than isopentenyl pyrophosphate...
  97. pmc Binding of phosphate and pyrophosphate ions at the active site of human angiogenin as revealed by X-ray crystallography
    D D Leonidas
    Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, United Kingdom
    Protein Sci 10:1669-76. 2001
    ..The Ang-pyrophosphate complex structure suggests how nucleoside pyrophosphate inhibitors might bind to Ang; this information may be useful for the design of Ang antagonists as potential anti-angiogenic drugs...
  98. ncbi Transient state kinetics of transcription elongation by T7 RNA polymerase
    Vasanti Subramanian Anand
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 281:35677-85. 2006
    ..The studies here provide a kinetic framework to investigate structure-function and fidelity of RNA synthesis and to further explore the role of the conformational change in nucleotide selection during RNA synthesis...
  99. ncbi Variety of nucleotide sugar transporters with respect to the interaction with nucleoside mono- and diphosphates
    Masatoshi Muraoka
    Stem Cell Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
    J Biol Chem 282:24615-22. 2007
    ..Possible physiological implications of these observations are discussed...
  100. ncbi A gel-free SNP genotyping method: bioluminometric assay coupled with modified primer extension reactions (BAMPER) directly from double-stranded PCR products
    Guo Hua Zhou
    Hitachi, Ltd, Central Research Laboratory, Tokyo, Japan
    Hum Mutat 24:155-63. 2004
    ..As all reactions can be carried out in a single tube, the method will be useful for realizing a fully automated genotyping system...
  101. pmc Association of ENPP1 gene polymorphisms with hand osteoarthritis in a Chuvasha population
    Eun Kyung Suk
    Gene Mapping Center, Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Arthritis Res Ther 7:R1082-90. 2005
    ..Thus, our data highlights variability of ENPP1 as an important genetic factor in the pathogenesis of idiopathic osteoarthritis...

Research Grants55

  1. Studies of Isoprenoid Biosynthesis
    CHARLES DALE POULTER; Fiscal Year: 2013
    ..kinase will be conducted, including the development of mutants capable of efficiently synthesizing longer chain diphosphates from the corresponding phosphates...
  2. Acquired mtDNA depletion and nucleoside reverse transciptase inhibitors
    William Lewis; Fiscal Year: 2010
    ..to monophosphates (-MP) by thymidine kinase (cytoplasmic TK1 and mitochondrial TK2 isoforms) and to diphosphates (-DP) by thymidylate kinase (TMPK)...
  3. Membrane Permeable Diphosphate Analogs Targeting Pathogen Isoprenoid Biosynthesis
    CAREN L MEYERS; Fiscal Year: 2013
    ..strategy to dramatically enhance the antimalarial properties of FPPS-targeting zoledronate (Aim 1) and linear diphosphates targeting IspG (Aim 2)...
  4. DEOXYRIBONUCLEOTIDE METABOLISM IN ESCHERICHIA COLI
    James Fuchs; Fiscal Year: 1992
    ..coli enzyme. A single enzyme catalyzes the reduction of all ribonucleotide diphosphates and the substrate specificity and overall activity is controlled by allosteric regulation...
  5. CYCLIC AMP RESISTANCE IN HYDROXYUREA RESISTANT CELLS
    Daniel Albert; Fiscal Year: 1990
    Ribonucleoside diphosphate reductase catalyzes the reduction of all four ribonucleoside diphosphates to their deoxynucleotide counterparts...
  6. Molecular Characterization of p53R2 in Head and Neck Ca
    Stuart Wong; Fiscal Year: 2004
    ..RR) - the enzyme that catalyzes the rate limiting step of DNA synthesis (conversion of ribonucleotide diphosphates to deoxyribonucleotide diphosphates)...
  7. NUCLEOSIDE PHOSPHATE ANALOGUES WITH BORONATED PHOSPHATES
    BERNARD SPIELVOGEL; Fiscal Year: 1991
    ..These compounds may be prepared by phosphorylation of nucleosides, nucleoside monophosphates and nucleoside diphosphates using a boronated phosphorylating agent...
  8. FAT NUCLEOSIDES AND NUCLEOTIDES
    RAMACHANDRA HOSMANE; Fiscal Year: 1990
    ..The mono- and diphosphate derivatives of nucleosides of category II will be prepared. The diphosphates will be polymerized by using the enzyme polynucleotide phosphorylase and the resulting homopolymer templates ..
  9. Intracellular Localization of Protein Prenylation
    Mark Brown; Fiscal Year: 2003
    ..mechanisms by which isoprenoids elicit their effects has been limited by the difficulty of getting isoprenoid diphosphates into live cells. Echelon Biosciences, Inc...
  10. PREDICTING THE RESPONSE OF TUMOR TO RADIATION
    Paul Okunieff; Fiscal Year: 1992
    ..Metabolic rate related parameters including: delta G (ATP) (free energy of hydrolysis); Mg++; free nucleotide diphosphates ((NDP)); T, of Pi and PCr; and ATPase and CK (creatine kinase) reaction rates will all be measured concurrently...
  11. Ribonucleotide Reductase: A Cancer Chemotherapy Target
    BARRY COOPERMAN; Fiscal Year: 2005
    ..The reaction catalyzed by RR, the reduction of nucleoside diphosphates (NDPs) to deoxynucleosides diphosphates (dNDPs), is the rate-limiting step in the de novo synthesis of dNTPs, ..
  12. Biochemical Mechanisms of Polyphosphate in Metabolism
    Arthur Kornberg; Fiscal Year: 2005
    ..Among poiy P functions are: kinase donor to glucose and nucleoside diphosphates, phosphate (P1) reservoir, divalent metal (Ca2+, Mg2+, Mn2+) chelator and component of a membrane complex in ..
  13. Engineering Terpene Synthases for Increased Productivity
    Jennifer Anthony; Fiscal Year: 2006
    ..Terpene synthases catalyze the cyclization of polyprenyl diphosphates to form a terpene backbone, which is the first committed step in all terpene biosynthesis...
  14. ISOPRENOID COMPOUNDS FOR BIOLOGICAL STUDIES
    CHARLES POULTER; Fiscal Year: 2001
    ..In phase I, (a) we optimized the "large scale" synthesis of simple linear C5, C10, C15, and C20 isoprenoid diphosphates, (b)developed procedures for purification and storage of isopentenyl diphosphate isomerase and farnesyl ..
  15. Renal Volume Measurement to Detect Early Kidney Disease
    W O NEILL; Fiscal Year: 2004
    ..abstract_text> ..
  16. MOLECULAR REGULATION OF ENDOTHELIAL NA K 2CL COTRANSPORT
    W O NEILL; Fiscal Year: 2002
    ..abstract_text> ..
  17. Immunotherapy with Gamma Delta T Cells for B Cell Tumors
    CRAIG MORITA; Fiscal Year: 2008
    ..abstract_text> ..
  18. Pyrophosphate in vascular calcification of renal failure
    W CHARLES O apos NEILL; Fiscal Year: 2010
    ..The results will establish a new paradigm for vascular calcification in renal failure and form the basis for important clinical trials in humans. ..
  19. ANTIGEN RECOGNITION BY GAMMA DELTA T CELLS
    CRAIG MORITA; Fiscal Year: 2007
    ..These studies will provide insights into nonpeptide antigen recognition by human gammadelta T cells and should help clarify their role in immunity and autoimmunity. ..
  20. Biomaterials (Mg/Zn/F-BCPs)for osteoporosis therapy
    Racquel LeGeros; Fiscal Year: 2006
    ..Hypothesis: Biomaterials with Zn, Mg and F ions in a calcium phosphate matrix (used as dietary supplement and ion releasing injectible 'implants') will improve bone health. ..
  21. Electroporation and transcutaneous extraction of drugs
    SATHYANARAYANA MURTHY; Fiscal Year: 2006
    ..Further we also plan to correlate the time course of unbound ciprofloxacin in dermal ECF with that in synovial fluid determined by microdialysis in rat. [unreadable] [unreadable] [unreadable]..
  22. Diadenosine Boranotetraphosph(on)ates as Antithrombotic Drugs
    Ivan Yanachkov; Fiscal Year: 2007
    ..The new drug will complement related drugs under development for arterial thrombosis. [unreadable] [unreadable] [unreadable]..
  23. The isoprenoid pathway from Toxoplasma gondii
    Silvia Moreno; Fiscal Year: 2007
    ..gondii, and perform structure-activity relationship studies to facilitate drug design. [unreadable] [unreadable] [unreadable]..
  24. Matrix proteins promote matrix vesicle mineralization
    Ann Rosenthal; Fiscal Year: 2007
    ..The ultimate goal of this work is to understand the pathogenesis of calcium crystal formation in articular cartilage, so that specific therapies for this disabling disease can be designed. ..
  25. Metalloprotein Structure, Function and Inhibition
    Eric Oldfield; Fiscal Year: 2008
    ....
  26. Reverse Transcription Free Microarray Analysis
    Ye Sun; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  27. Protein Structure/Function by Quantum Chemistry and NMR
    Eric Oldfield; Fiscal Year: 2006
    ..The fourth and final specific aim is to use NMR and quantum chemical methods to develop improved approaches for quantitative structure-activity relationship studies (quantum QSAR applications). ..
  28. NMR STUDIES OF ENZYMES/METAL SUBSTRATE INTERACTIONS
    ALBERT MILDVAN; Fiscal Year: 2004
    ..The purpose of studying multiple enzymes which catalyze reactions of the same class is to elucidate general principles of enzyme chemistry and to develop new approaches to enzyme structure and mechanism. ..
  29. Pathogen-sensing with novel poly(aryleneethynylene)s
    UWE BUNZ; Fiscal Year: 2003
    ..The sensing event should be detectable by simple inspection, or in more sophisticated cases a simple fluorescence spectrometer will be utilized. ..
  30. ACCELERATING IRON CHELATION FROM SERUM TRANSFERRIN
    WESLEY HARRIS; Fiscal Year: 2004
    ..This is a collaborative project involving faculty with expertise in molecular biology, solution kinetics, organic synthesis, and computational chemistry. ..
  31. MOLECULAR BASIS OF CALCIUM PYROPHOSPHATE DEPOSITION
    Ikuko Masuda; Fiscal Year: 2002
    ..It is hoped that these studies will lead to therapies to reduce ePPi accumulation and reduce subsequent cartilage degeneration. ..
  32. Docosanol and Acyclovir for Treating Genital Herpes
    LAURA POPE; Fiscal Year: 2003
    ..If proven safe and effective, it would provide a new single agent treatment as well as allow concurrent use with acyclovir. PROPOSED COMMERCIAL APPLICATION: Topical treatment for recurrent genital herpes infections. ..
  33. CHEMISTRY AND BIOLOGY OF INSULIN-LIKE VANADIUM COMPOUNDS
    DEBBIE CRANS; Fiscal Year: 2003
    ..The proposed studies will lead to an in-depth understanding of both the chemical and pharmacological properties of known and new vanadium compounds inducing insulin-like action. ..
  34. Novel phospholipases C in trypanosomatids
    Silvia Moreno; Fiscal Year: 2009
    ..abstract_text> ..
  35. Chondrocyte S100/Calgranulins and RAGE in Osteoarthritis
    Robert Terkeltaub; Fiscal Year: 2009
    ..Completion of the work will reveal new potential targets for therapeutic intervention in OA by disconnecting inflammation from deleterious alteration of chondrocyte differentiation and function. ..
  36. TWO X-LINKED GENES THAT REGULATE MINERAL HOMEOSTASIS
    Michael Whyte; Fiscal Year: 2002
    ....
  37. Mechanisms of antibiotic action in Neisseria gonorrhoeae.
    Robert A Nicholas; Fiscal Year: 2010
    ..Together, these projects utilize genetic, biochemical, biophysical, and proteomics approaches to generate new insights into the mechanisms of antibiotic action in N. gonorrhoeae. ..
  38. NMR STUDIES OF RESPIRATORY PROTEINS AND MODEL SYSTEMS
    Eric Oldfield; Fiscal Year: 2001
    ..Oldfield's refined structures of the proteins, the energetics of binding of small molecules to hemes will be studied, which he hypothesizes are dominated by hydrogen bonding interactions in the cavity. ..
  39. Trafficking/signaling of P2Y receptors in polarized epithelial cells
    Robert A Nicholas; Fiscal Year: 2011
    ..Our studies will provide novel and important information on the cellular mechanisms of P2Y receptor targeting and the regulation of P2Y receptor signaling activity in epithelial cells. ..
  40. RADIATION AND DRUG EFFECTS ON TUMOR CELL SUBPOPULATIONS
    Dietmar Siemann; Fiscal Year: 2001
    ..Ultimately we hope that these approaches can be reliably developed to an extent where the use of human tumor biopsy material may lead to radiotherapy treatments more tailored to the individual patient. ..
  41. ACIDOCALCISOMES AND TOXOPLASMA GONDII
    Silvia Moreno; Fiscal Year: 2003
    ..gondii H+-pyrophosphatase; specific aim 3: investigation of the role of acidocalcisomes in intracellular Ca2+ and pH homeostasis. ..
  42. Elementary Steps in DNA Polymerization
    Kenneth Johnson; Fiscal Year: 2008
    ..abstract_text> ..
  43. ANK and Joint Ankylosis in Spondylitis
    Robert Terkeltaub; Fiscal Year: 2008
    ..These studies will identify novel targets for enthesopathic bone fusion in spondyloarthritis. ..
  44. DNA/RNA Quadruplexes: Structure, Stability, Interactions
    RICHARD SHAFER; Fiscal Year: 2006
    ..This combination of approaches will afford a detailed examination of the conformation and interaction properties of the very important nucleic acid species. [unreadable] [unreadable]..
  45. PTHRP 1 to 173 IN CHONDROCYTES
    Robert Terkeltaub; Fiscal Year: 2005
    ..Completion of these studies will indicate not only how PTHrP 1-173 can modulate OA, but also how functional properties of the PTHrP 1-173 C-terminal 140-173 domain can be harnessed to promote cartilage repair. ..
  46. Novel inhibitors of the replication of poxviruses
    Johan Neyts; Fiscal Year: 2004
    ..The final goal of this application is to advance a compound for the treatment of (systemic or cutaneous) poxvirus infections to the initial stages of development, i.e., selecting an IND candidate. ..
  47. High-throughput Sequencing by Pyrosequencing
    Mostafa Ronaghi; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  48. Novel Anti-Viral Agents for Treating Lassa Fever
    Philip Thorpe; Fiscal Year: 2007
    ..The information generated by these studies may also extend to other viruses that could be used for bioterrorism purposes. ..
  49. TRANSGLUTAMINASE PROMOTES CPPD DISEASE IN AGING JOINTS
    Ann Rosenthal; Fiscal Year: 2007
    ..These studies will contribute to our understanding of CPPD deposition disease, so that effective therapies for this common and disabling form of arthritis can be designed. ..
  50. NMR STUDIES OF RESPIRATORY PROTEINS AND MODEL SYSTEMS
    Eric Oldfield; Fiscal Year: 2003
    ..Oldfield's refined structures of the proteins, the energetics of binding of small molecules to hemes will be studied, which he hypothesizes are dominated by hydrogen bonding interactions in the cavity. ..
  51. Novel Antithrombotic Diadenosine Tetraphosphate Analogs
    Ivan Yanachkov; Fiscal Year: 2007
    ..The new drug will complement related drugs under development for arterial thrombosis. [unreadable] [unreadable] [unreadable]..
  52. KINETIC ANALYSIS OF MICROTUBULE-DEPENDENT ATPASES
    Kenneth Johnson; Fiscal Year: 2003
    ..The combination of approaches outlined here will provide rigorous and direct information to define the structural and mechanistic basis for force production by kinesin. ..
  53. ENZYMATIC LUMINESCENCE microRNA ASSAY
    Ye Sun; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  54. CaP, crystal responsible for idiopathic nephrolithasis
    Saeed Khan; Fiscal Year: 2008
    ..Type density and size must conform to limits and specifications provided in the PHS 398Instructions. RESEARCH GRANT TABLE OF CONTENTS Page Numbers Face Page 1 Description, ..
  55. TISSUE-CRYSTAL INTERACTION CA OXALATE NEPHROLITHIASIS
    Saeed Khan; Fiscal Year: 2003
    ..We can follow various events of nephrolithiasis in the animal model and cats explore the outcome of renal epithelial cell exposure to oxalate and calcium oxalate crystals in the cell cultures. ..