carbon isotopes

Summary

Summary: Stable carbon atoms that have the same atomic number as the element carbon, but differ in atomic weight. C-13 is a stable carbon isotope.

Top Publications

  1. ncbi Getting to the fat of the matter: models, methods and assumptions for dealing with lipids in stable isotope analyses
    David M Post
    Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT 06520 8105, USA
    Oecologia 152:179-89. 2007
  2. ncbi 1H, 13C and 15N chemical shift referencing in biomolecular NMR
    D S Wishart
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    J Biomol NMR 6:135-40. 1995
  3. ncbi Using stable isotopes to investigate individual diet specialization in California sea otters (Enhydra lutris nereis)
    Seth D Newsome
    Carnegie Institution of Washington, Geophysical Laboratory, 5251 Broad Branch Road NW, Washington, D C 20015, USA
    Ecology 90:961-74. 2009
  4. pmc Carbon isotope compositions of terrestrial C3 plants as indicators of (paleo)ecology and (paleo)climate
    Matthew J Kohn
    Department of Geosciences, Boise State University, Boise, ID 83725, USA
    Proc Natl Acad Sci U S A 107:19691-5. 2010
  5. ncbi Can stable isotope ratios provide for community-wide measures of trophic structure?
    Craig A Layman
    Marine Science Program, Department of Biological Sciences, 3000 NE 151st Street, North Miami, Florida 33181, USA
    Ecology 88:42-8. 2007
  6. ncbi (13)C-based metabolic flux analysis
    Nicola Zamboni
    Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland
    Nat Protoc 4:878-92. 2009
  7. pmc Analysis of cancer metabolism by imaging hyperpolarized nuclei: prospects for translation to clinical research
    John Kurhanewicz
    Department of Radiology and Biomedical Imaging, University of California at San Francisco, San Francisco, CA 94158 2330, USA
    Neoplasia 13:81-97. 2011
  8. ncbi Detecting tumor response to treatment using hyperpolarized 13C magnetic resonance imaging and spectroscopy
    Sam E Day
    Cancer Research UK, Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK
    Nat Med 13:1382-7. 2007
  9. ncbi Magnetic resonance imaging of pH in vivo using hyperpolarized 13C-labelled bicarbonate
    Ferdia A Gallagher
    Cancer Research UK, Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK
    Nature 453:940-3. 2008
  10. pmc ¹³C metabolic flux analysis identifies an unusual route for pyruvate dissimilation in mycobacteria which requires isocitrate lyase and carbon dioxide fixation
    Dany J V Beste
    School of Biomedical and Molecular Sciences, University of Surrey, Guildford, United Kingdom
    PLoS Pathog 7:e1002091. 2011

Detail Information

Publications402 found, 100 shown here

  1. ncbi Getting to the fat of the matter: models, methods and assumptions for dealing with lipids in stable isotope analyses
    David M Post
    Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT 06520 8105, USA
    Oecologia 152:179-89. 2007
    ..Both direct lipid extraction and mathematical normalization reduce biases in delta(13)C, but mathematical normalization simplifies sample preparation and better preserves the integrity of samples for delta(15)N analysis...
  2. ncbi 1H, 13C and 15N chemical shift referencing in biomolecular NMR
    D S Wishart
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    J Biomol NMR 6:135-40. 1995
    ....
  3. ncbi Using stable isotopes to investigate individual diet specialization in California sea otters (Enhydra lutris nereis)
    Seth D Newsome
    Carnegie Institution of Washington, Geophysical Laboratory, 5251 Broad Branch Road NW, Washington, D C 20015, USA
    Ecology 90:961-74. 2009
    ....
  4. pmc Carbon isotope compositions of terrestrial C3 plants as indicators of (paleo)ecology and (paleo)climate
    Matthew J Kohn
    Department of Geosciences, Boise State University, Boise, ID 83725, USA
    Proc Natl Acad Sci U S A 107:19691-5. 2010
    ..4 Ma show no isotopic evidence for dense forests. Consideration of global plant biomass indicates that average δ(13)C of C3 plants is commonly overestimated by approximately 2‰...
  5. ncbi Can stable isotope ratios provide for community-wide measures of trophic structure?
    Craig A Layman
    Marine Science Program, Department of Biological Sciences, 3000 NE 151st Street, North Miami, Florida 33181, USA
    Ecology 88:42-8. 2007
    ..Building from extensive applications of stable isotope ratios by ecologists, the community-wide metrics may provide a new perspective on food web structure, function, and dynamics...
  6. ncbi (13)C-based metabolic flux analysis
    Nicola Zamboni
    Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland
    Nat Protoc 4:878-92. 2009
    ..The presented protocols take 5-10 d, have been used extensively in the past decade and are exemplified here for the central metabolism of Escherichia coli...
  7. pmc Analysis of cancer metabolism by imaging hyperpolarized nuclei: prospects for translation to clinical research
    John Kurhanewicz
    Department of Radiology and Biomedical Imaging, University of California at San Francisco, San Francisco, CA 94158 2330, USA
    Neoplasia 13:81-97. 2011
    ..Nevertheless, there are no fundamental barriers to rapid translation of this exciting technology to clinical research and perhaps clinical care...
  8. ncbi Detecting tumor response to treatment using hyperpolarized 13C magnetic resonance imaging and spectroscopy
    Sam E Day
    Cancer Research UK, Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK
    Nat Med 13:1382-7. 2007
    ..The technique could provide a new way to assess tumor responses to treatment in the clinic...
  9. ncbi Magnetic resonance imaging of pH in vivo using hyperpolarized 13C-labelled bicarbonate
    Ferdia A Gallagher
    Cancer Research UK, Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK
    Nature 453:940-3. 2008
    ....
  10. pmc ¹³C metabolic flux analysis identifies an unusual route for pyruvate dissimilation in mycobacteria which requires isocitrate lyase and carbon dioxide fixation
    Dany J V Beste
    School of Biomedical and Molecular Sciences, University of Surrey, Guildford, United Kingdom
    PLoS Pathog 7:e1002091. 2011
    ..This study also provides a platform for further studies into the metabolism of M. tuberculosis using ¹³C-MFA...
  11. pmc Plant breeding and drought in C3 cereals: what should we breed for?
    J L Araus
    Unitat de Fisiologia Vegetal, Facultat de Biologia, Universitat de Barcelona, Spain
    Ann Bot 89:925-40. 2002
    ..Alternatively, extending the duration of stem elongation without changing the timing of anthesis would increase the number of grains per spike and the harvest index without changing the amount of water utilized by the crop...
  12. pmc Hyperpolarized 13C dehydroascorbate as an endogenous redox sensor for in vivo metabolic imaging
    Kayvan R Keshari
    Department of Radiology and Biomedical Imaging, University of California San Francisco, 1700 4th Street, Byers Hall 203, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 108:18606-11. 2011
    ..More broadly, these findings suggest a prognostic role for this new redox sensor in determining vulnerability of both normal and abnormal tissues to ROS...
  13. ncbi Post-photosynthetic fractionation of stable carbon isotopes between plant organs--a widespread phenomenon
    Franz W Badeck
    Potsdam Institute for Climate Impact Research PIK, P O Box 601203, 14412 Potsdam, Germany
    Rapid Commun Mass Spectrom 19:1381-91. 2005
    ..We discuss the main hypotheses that can explain the observed inter-organ differences in delta13C...
  14. ncbi Quantitative analysis of the microbial metabolome by isotope dilution mass spectrometry using uniformly 13C-labeled cell extracts as internal standards
    Liang Wu
    Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands
    Anal Biochem 336:164-71. 2005
    ..In conclusion, the method presented leads to less workload, more robustness, and a higher precision in metabolome analysis...
  15. ncbi Effects of growth and tissue type on the kinetics of 13C and 15N incorporation in a rapidly growing ectotherm
    Kimberly J Reich
    Archie Carr Center for Sea Turtle Research and Department of Zoology, University of Florida, Gainesville, FL 32611, USA
    Oecologia 155:651-63. 2008
    ..The increasing reliance on non-invasive and non-destructive sampling in animal isotopic ecology requires that we recognize and understand why different tissues differ in isotopic discrimination factors...
  16. pmc Kinetic modeling of hyperpolarized 13C label exchange between pyruvate and lactate in tumor cells
    Timothy H Witney
    Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, United Kingdom
    J Biol Chem 286:24572-80. 2011
    ..The kinetic model should be useful for modeling the exchange reaction in vivo, particularly as this technique progresses to the clinic...
  17. ncbi Central metabolic fluxes in the endosperm of developing maize seeds and their implications for metabolic engineering
    Ana P Alonso
    Department of Plant Biology, Michigan State University, East Lansing, MI 48824, USA
    Metab Eng 13:96-107. 2011
    ....
  18. pmc Method for determining molar concentrations of metabolites in complex solutions from two-dimensional 1H-13C NMR spectra
    Ian A Lewis
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, Wisconsin 53706, USA
    Anal Chem 79:9385-90. 2007
    ..2% technical error under nearly ideal conditions. We propose FMQ by NMR as a practical alternative to 1D (1)H NMR for metabolomics studies in which 50-mg (extract dry weight) samples can be obtained...
  19. ncbi The ERECTA gene regulates plant transpiration efficiency in Arabidopsis
    Josette Masle
    Environmental Biology Group, Research School of Biological Sciences, The Australian National University, Canberra, ACT 2601, Australia
    Nature 436:866-70. 2005
    ..Mechanisms include, but are not limited to, effects on stomatal density, epidermal cell expansion, mesophyll cell proliferation and cell-cell contact...
  20. pmc Evaluation of 13C isotopic tracers for metabolic flux analysis in mammalian cells
    Christian M Metallo
    Department of Chemical Engineering, Massachusetts Institute of Technology, Building 56 Room 469C, 77 Massachusetts Ave, Cambridge, MA 02139, United States
    J Biotechnol 144:167-74. 2009
    ..These results provide valuable, quantitative information on the performance of (13)C-labeled substrates and can aid in the design of more informative MFA experiments in mammalian cell culture...
  21. pmc Noninvasive detection of target modulation following phosphatidylinositol 3-kinase inhibition using hyperpolarized 13C magnetic resonance spectroscopy
    Christopher S Ward
    Department of Radiology and Biomedical Imaging, University of California at San Francisco, San Francisco, California 94158, USA
    Cancer Res 70:1296-305. 2010
    ....
  22. ncbi Source partitioning using stable isotopes: coping with too many sources
    Donald L Phillips
    U S Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, 200 SW 35th St, Corvallis, OR 97333, USA
    Oecologia 136:261-9. 2003
    ..A computer program (IsoSource) to perform these calculations for user-specified data is available at http://www.epa.gov/wed/pages/models.htm...
  23. pmc Measuring intracellular pH in the heart using hyperpolarized carbon dioxide and bicarbonate: a 13C and 31P magnetic resonance spectroscopy study
    Marie A Schroeder
    Cardiac Metabolism Research Group, Department of Physiology, Anatomy and Genetics, University of Oxford, Sherrington Building, Parks Road, Oxford OX1 3PT, UK
    Cardiovasc Res 86:82-91. 2010
    ..The aim of this study was to evaluate the potential of hyperpolarized [1-(13)C]pyruvate, coupled with (13)C magnetic resonance spectroscopy (MRS), to measure pH(i) in the healthy and diseased heart...
  24. ncbi Characterization of isotopic abundance measurements in high resolution FT-ICR and Orbitrap mass spectra for improved confidence of metabolite identification
    Ralf J M Weber
    Centre for Systems Biology, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    Anal Chem 83:3737-43. 2011
    ..g., 10 carbons), we demonstrate that RIA measurements do improve automated peak annotation, increasing the number of single empirical formula assignments by >3-fold compared to using accurate mass alone...
  25. pmc Selective 13C labeling of nucleotides for large RNA NMR spectroscopy using an E. coli strain disabled in the TCA cycle
    Chandar S Thakur
    Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Organization, University of Maryland, 1115 Biomolecular Sciences Bldg 296, College Park, MD 20742 3360, USA
    J Biomol NMR 48:179-92. 2010
    ..Even under conditions of slow tumbling, all the expected carbon correlations were observed, which indicates this approach of using nucleotides obtained from DL323 E. coli will be applicable to high molecular weight RNA systems...
  26. ncbi A simple method to predict protein flexibility using secondary chemical shifts
    Mark V Berjanskii
    Departments of Computing Science and Biological Sciences, University of Alberta, Edmonton, AB, Canada T6G 2E8
    J Am Chem Soc 127:14970-1. 2005
    ..Further, we show that this chemical shift method is able to quantitatively predict per-residue RMSD values (from both MD simulations and NMR structural ensembles) as well as model-free backbone order parameters...
  27. ncbi A critical evaluation of intrapopulation variation of delta13C and isotopic evidence of individual specialization
    Blake Matthews
    Water and Watershed Research Program, Department of Biology, University of Victoria, P O Box 3020 STN CSC, Victoria, British Columbia, Canada, V85 3N5
    Oecologia 140:361-71. 2004
    ..Our analyses fundamentally challenge how we interpret the intrapopulation isotopic variance of delta13C, and how we evaluate isotopic evidence of individual specialization...
  28. pmc In vivo assessment of pyruvate dehydrogenase flux in the heart using hyperpolarized carbon-13 magnetic resonance
    Marie A Schroeder
    Cardiac Metabolism Research Group, Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford OX1 3PT, United Kingdom
    Proc Natl Acad Sci U S A 105:12051-6. 2008
    ....
  29. pmc Hyperpolarized 13C lactate, pyruvate, and alanine: noninvasive biomarkers for prostate cancer detection and grading
    Mark J Albers
    Department of Bioengineering, University of California San Francisco, San Francisco, California 94158 2330, USA
    Cancer Res 68:8607-15. 2008
    ....
  30. ncbi The effect of cold-induced increased metabolic rate on the rate of 13C and 15N incorporation in house sparrows (Passer domesticus)
    S A Carleton
    Department of Zoology and Physiology, University of Wyoming, Laramie, WY 82072 3166, USA
    Oecologia 144:226-32. 2005
    ..We conclude that the relationship between metabolic rate and the rate of isotopic incorporation into an animal's tissues is indirect. It is probably mediated by protein turnover and thus more complex than previous studies have assumed...
  31. ncbi Factors that influence assimilation rates and fractionation of nitrogen and carbon stable isotopes in avian blood and feathers
    Stuart Bearhop
    Ornithology Group, Graham Kerr Building, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland
    Physiol Biochem Zool 75:451-8. 2002
    ..There is a need for more laboratory-based experimental isotope studies in order to address further questions that this study has raised...
  32. ncbi Stable isotope assisted assignment of elemental compositions for metabolomics
    Adrian D Hegeman
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin 53706, USA
    Anal Chem 79:6912-21. 2007
    ..These results demonstrate that metabolic labeling can be used to provide additional constraints for higher confidence formula assignments over an extended mass range...
  33. ncbi 13C metabolic flux analysis in complex systems
    Nicola Zamboni
    Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland
    Curr Opin Biotechnol 22:103-8. 2011
    ..In the future, this practice will be complemented by novel modeling frameworks to assay particular fluxes within a network by stable isotopic tracer for targeted validation of well-defined hypotheses...
  34. pmc Real-time assessment of Krebs cycle metabolism using hyperpolarized 13C magnetic resonance spectroscopy
    Marie A Schroeder
    Cardiac Metabolism Research Group, Department of Physiology, Anatomy and Genetics, Sherrington Bldg, University of Oxford, Parks Rd, Oxford, UK
    FASEB J 23:2529-38. 2009
    ..These results showed that the control and fluxes of the Krebs cycle in heart disease can be studied using hyperpolarized [2-(13)C]pyruvate...
  35. pmc Metabolic network fluxes in heterotrophic Arabidopsis cells: stability of the flux distribution under different oxygenation conditions
    Thomas C R Williams
    Department of Plant Sciences, University of Oxford, Oxford, United Kingdom
    Plant Physiol 148:704-18. 2008
    ....
  36. pmc The cycling of acetyl-coenzyme A through acetylcarnitine buffers cardiac substrate supply: a hyperpolarized 13C magnetic resonance study
    Marie A Schroeder
    Cardiac Metabolism Research Group, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK
    Circ Cardiovasc Imaging 5:201-9. 2012
    ..The aim of this study was to use the metabolic tracer hyperpolarized [2-(13)C]pyruvate with magnetic resonance spectroscopy to determine whether carnitine acetyltransferase facilitates carbohydrate oxidation in the heart...
  37. pmc Analysis of metabolic flux phenotypes for two Arabidopsis mutants with severe impairment in seed storage lipid synthesis
    Joachim Lonien
    Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA
    Plant Physiol 151:1617-34. 2009
    ..No such limited compensatory bypass could be observed in pkpbeta(1)pkpalpha...
  38. pmc Carbon metabolism of enterobacterial human pathogens growing in epithelial colorectal adenocarcinoma (Caco-2) cells
    Andreas Götz
    Lehrstuhl fur Mikrobiologie, Biozentrum der Universität Würzburg, Wurzburg, Germany
    PLoS ONE 5:e10586. 2010
    ..Surprisingly, the metabolism of the host cells, as judged by the efficiency of (13)C-incorporation into host cell amino acids, was not significantly affected by the infection with either of these intracellular pathogens...
  39. ncbi Nodulation enhances dark CO₂ fixation and recycling in the model legume Lotus japonicus
    Mariangela N Fotelli
    Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece
    J Exp Bot 62:2959-71. 2011
    ..A potential mechanism is proposed for reducing carbon losses during SNF by the direct reincorporation of CO(2) respired by nodules and the transport and metabolism of C-containing metabolites in hypergeous organs...
  40. pmc Simultaneous analysis of microbial identity and function using NanoSIMS
    Tianlun Li
    Cemagref, Unité de Recherche Hydrosystèmes et Bioprocédés, Parc de Tourvoie, BP 44, 92163 Antony cedex, France
    Environ Microbiol 10:580-8. 2008
    ..The applicability of this new technique to the study of in situ ecophysiology of uncultured microorganisms within complex microbial communities is illustrated...
  41. pmc Tissue turnover rates and isotopic trophic discrimination factors in the endothermic teleost, pacific bluefin tuna (Thunnus orientalis)
    Daniel J Madigan
    Tuna Research and Conservation Center, Hopkins Marine Station of Stanford University, Pacific Grove, California, United States of America
    PLoS ONE 7:e49220. 2012
    ..These results allow more accurate interpretation of field data and dramatically improve our ability to use stable isotope data from wild tunas to better understand their migration patterns and trophic ecology...
  42. ncbi Quantitative approaches to the analysis of stable isotope food web data
    Stephanie N Schmidt
    Center for Limnology, University of Wisconsin, 680 North Park Street, Madison, Wisconsin 53706, USA
    Ecology 88:2793-802. 2007
    ..As more ecologists incorporate a food web perspective into ecosystem analysis, these statistical tools can provide a basis for quantifying directional food web differences from standard isotope data...
  43. pmc Compressed sensing for resolution enhancement of hyperpolarized 13C flyback 3D-MRSI
    Simon Hu
    Department of Radiology, University of California, Box 2512, 1700 4th Street, QB3 Building, Suite 102, San Francisco, CA 94158 2512, USA
    J Magn Reson 192:258-64. 2008
    ..Phantom tests validated the accuracy of the compressed sensing approach and initial mouse experiments demonstrated in vivo feasibility...
  44. ncbi Drought effects on allocation of recent carbon: from beech leaves to soil CO2 efflux
    Nadine K Ruehr
    Institute of Plant Science, ETH Zurich, Zurich, Switzerland
    New Phytol 184:950-61. 2009
    ..This will probably affect soil biogeochemical cycling, with potential consequences including slower soil nitrogen cycling and changes in C-sequestration potential under future climate conditions...
  45. pmc Kinetic modeling of hyperpolarized 13C1-pyruvate metabolism in normal rats and TRAMP mice
    Matthew L Zierhut
    UCSF UCB Joint Graduate Group in Bioengineering, San Francisco, CA 94143 2532, USA
    J Magn Reson 202:85-92. 2010
    ....
  46. ncbi Real-time detection of central carbon metabolism in living Escherichia coli and its response to perturbations
    Sebastian Meier
    Carlsberg Laboratory, Copenhagen, Denmark
    FEBS Lett 585:3133-8. 2011
    ..Alterations in central carbon metabolism depending on the growth phase or upon chemical perturbations are visualized with minimal data processing by instantaneous observation of cellular reactions...
  47. pmc Integrating stomach content and stable isotope analyses to quantify the diets of pygoscelid penguins
    Michael J Polito
    Department of Biology and Marine Biology, University of North Carolina Wilmington, Wilmington, North Carolina, United States of America
    PLoS ONE 6:e26642. 2011
    ....
  48. pmc Predicting outcomes of steady-state ¹³C isotope tracing experiments using Monte Carlo sampling
    Jan Schellenberger
    Bioinformatics and Systems Biology Program, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0419, USA
    BMC Syst Biol 6:9. 2012
    ..Unlike previous work, this method does not require assumption of the flux distribution beforehand...
  49. ncbi Isotopic and genetic evidence for culturally inherited site fidelity to feeding grounds in southern right whales (Eubalaena australis)
    Luciano O Valenzuela
    Department of Biology, University of Utah, 257 South 1400 East, Salt Lake City, UT 84112, USA
    Mol Ecol 18:782-91. 2009
    ....
  50. ncbi Tumor imaging using hyperpolarized 13C magnetic resonance spectroscopy
    Kevin M Brindle
    Cancer Research UK, Cambridge Research Institute, and Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom
    Magn Reson Med 66:505-19. 2011
    ..If the challenges in translating this technique to human imaging can be overcome, then it has the potential to significantly alter the management of cancer patients...
  51. ncbi Metabolic flux analysis of CHO cells at growth and non-growth phases using isotopic tracers and mass spectrometry
    Woo Suk Ahn
    Department of Chemical Engineering, Metabolic Engineering and Systems Biology Laboratory, University of Delaware, 150 Academy Street, Newark, DE 19716, USA
    Metab Eng 13:598-609. 2011
    ..The results presented here provide a solid foundation for future studies of CHO cell metabolism for applications such as cell line development and medium optimization for high-titer production of recombinant proteins...
  52. pmc Glucose-1-phosphate transport into protoplasts and chloroplasts from leaves of Arabidopsis
    Joerg Fettke
    Mass Spectrometry of Biopolymers, University of Potsdam, 14476 Potsdam Golm, Germany
    Plant Physiol 155:1723-34. 2011
    ..Mutants lacking the plastidial phosphoglucomutase contain a small yet significant amount of transitory starch...
  53. ncbi Deuteration of a molecular probe for DNP hyperpolarization--a new approach and validation for choline chloride
    Hyla Allouche-Arnon
    Department of Radiology, Hadassah Hebrew University Medical Center, Jerusalem, Israel
    Contrast Media Mol Imaging 6:499-506. 2011
    ..It is expected that the use of deuterated carbon-13 positions as reporting hyperpolarized nuclei will substantially expand the library of agents for DNP-MR...
  54. ncbi The metabolic flux phenotype of heterotrophic Arabidopsis cells reveals a complex response to changes in nitrogen supply
    Shyam K Masakapalli
    Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK
    Plant J 74:569-82. 2013
    ..These conclusions highlight the complexity of the metabolic response to a change in nitrogen nutrition...
  55. pmc Increasing the multiplexing capacity of TMTs using reporter ion isotopologues with isobaric masses
    Graeme C McAlister
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, United States
    Anal Chem 84:7469-78. 2012
    ..Considering the structure of the TMT reporter ion, we believe this work serves as a blueprint for expanding the multiplexing capacity of the TMT reagents to at least 10-plex and possibly up to 18-plex...
  56. pmc Population-level metrics of trophic structure based on stable isotopes and their application to invasion ecology
    Michelle C Jackson
    School of Biological and Chemical Sciences, Queen Mary University of London, London, England
    PLoS ONE 7:e31757. 2012
    ..Although we have demonstrated their applicability specifically using a detailed temporal dataset of species invasion in a lake, they have a wide array of potential ecological applications...
  57. pmc Demonstration of the ethylmalonyl-CoA pathway by using 13C metabolomics
    Rémi Peyraud
    Institute of Microbiology, Eidgenössiche Technische Hochschule Zurich, 8093 Zurich, Switzerland
    Proc Natl Acad Sci U S A 106:4846-51. 2009
    ..extorquens AM1 has major implications for the physiology of these methylotrophs and their role in nature, and it also provides a common ground for C1 and C2 compound assimilation in isocitrate lyase-negative bacteria...
  58. ncbi Carbon isotope fractionation of amino acids in fish muscle reflects biosynthesis and isotopic routing from dietary protein
    Kelton W McMahon
    Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, Joint Program in Oceanography and Ocean Engineering, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA
    J Anim Ecol 79:1132-41. 2010
    1. Analysis of stable carbon isotopes is a valuable tool for studies of diet, habitat use and migration...
  59. pmc Ontogenetic and among-individual variation in foraging strategies of northeast Pacific white sharks based on stable isotope analysis
    Sora L Kim
    Department of Earth and Planetary Sciences, University of California Santa Cruz, Santa Cruz, California, USA
    PLoS ONE 7:e45068. 2012
    ..Our data reveal substantial ontogenetic and individual dietary variation within a white shark population...
  60. ncbi Stable-isotope probing of the polycyclic aromatic hydrocarbon-degrading bacterial guild in a contaminated soil
    Maiysha D Jones
    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, The University of North Carolina at Chapel Hill, CB 7431 Chapel Hill, NC 27599 7431, USA
    Environ Microbiol 13:2623-32. 2011
    ....
  61. ncbi Spatial distributions of carbon, nitrogen and sulfur isotope ratios in human hair across the central United States
    Luciano O Valenzuela
    Department of Biology, University of Utah, 257 South 1400 East, Salt Lake City, UT 84112, USA
    Rapid Commun Mass Spectrom 25:861-8. 2011
    ..The accuracy of the map was tested using hair samples not included in its generation. We conclude that sulfur isotope analysis may represent a new tool to investigate the movements and/or region-of-origin of humans...
  62. pmc Metabolic turnover analysis by a combination of in vivo 13C-labelling from 13CO2 and metabolic profiling with CE-MS/MS reveals rate-limiting steps of the C3 photosynthetic pathway in Nicotiana tabacum leaves
    Tomohisa Hasunuma
    Research institute of Innovative Technology for the Earth RITE, 9 2 Kizugawadai, Kizugawa shi, Kyoto 619 0292 Japan
    J Exp Bot 61:1041-51. 2010
    ..The pool size of G1P is 12 times lower than that of G6P. These results indicate that the conversion of G6P to G1P is one of the rate-limiting steps in the sugar phosphate pathway...
  63. ncbi Glucose 1-phosphate is efficiently taken up by potato (Solanum tuberosum) tuber parenchyma cells and converted to reserve starch granules
    Joerg Fettke
    Institute of Biochemistry and Biology, Mass Spectrometry of Biopolymers, University of Potsdam, Karl Liebknecht Str 24 25, Building 20, D 14476 Potsdam Golm, Germany
    New Phytol 185:663-75. 2010
    ..Two distinct paths of reserve starch biosynthesis are proposed that explain, at a biochemical level, the phenotype of several transgenic plant lines...
  64. ncbi Lipid corrections in carbon and nitrogen stable isotope analyses: comparison of chemical extraction and modelling methods
    John M Logan
    Zoology Department, Large Pelagics Research Center, University of New Hampshire, Durham, NH 03824, USA
    J Anim Ecol 77:838-46. 2008
    ..By analysing a subset of samples before and after lipid extraction, models can be applied to the species and tissues of interest that will improve estimates of dietary sources using stable isotopes...
  65. ncbi Stationary versus non-stationary (13)C-MFA: a comparison using a consistent dataset
    Stephan Noack
    Institut fur Biotechnologie, Forschungszentrum Julich, Leo Brandt Straße, Julich, NRW, Germany
    J Biotechnol 154:179-90. 2011
    ..Taking further criteria on the experimental and computational part into consideration, the current limitations of both methods are demonstrated and possible pitfalls are concluded...
  66. ncbi Seabird satellite tracking validates the use of latitudinal isoscapes to depict predators' foraging areas in the Southern Ocean
    Audrey Jaeger
    Centre d Etudes Biologiques de Chize, UPR 1934 du CNRS, 79360 Villiers en Bois, France
    Rapid Commun Mass Spectrom 24:3456-60. 2010
    ..The combination of device deployments with sampling of relevant tissues for isotopic analysis appears to be a powerful tool for investigating consumers' isoscapes at various spatio-temporal scales...
  67. ncbi New guidelines for delta13C measurements
    Tyler B Coplen
    U S Geological Survey, 431 National Center, Reston, Virginia 20192, USA
    Anal Chem 78:2439-41. 2006
    ..Uncertainties of other reference material values on this scale are improved by factors up to two or more, and the values of some have been notably shifted: the delta13C of NBS 22 oil is -30.03 per thousand...
  68. pmc Growth of wildtype and mutant E. coli strains in minimal media for optimal production of nucleic acids for preparing labeled nucleotides
    Chandar S Thakur
    Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Organization, University of Maryland, 1115 Biomolecular Sciences, Bldg 296, College Park, MD 20742 3360, USA
    Appl Microbiol Biotechnol 88:771-9. 2010
    ..These studies should benefit efforts to make selective 13C-15N isotopic-labeled nucleotides for synthesizing biologically important RNAs...
  69. ncbi Ultralong spin coherence time in isotopically engineered diamond
    Gopalakrishnan Balasubramanian
    Physikalisches Institut, Universitat Stuttgart, 70550 Stuttgart, Germany
    Nat Mater 8:383-7. 2009
    ..We also show that single electron spins in the same isotopically engineered CVD diamond can be used to detect external magnetic fields with a sensitivity reaching 4 nT Hz(-1/2) and subnanometre spatial resolution...
  70. ncbi NMR determination of photorespiration in intact leaves using in vivo 13CO2 labeling
    Lynette Cegelski
    Department of Chemistry, Washington University, St Louis, MO 63130, USA
    J Magn Reson 178:1-10. 2006
    ..The net carbon assimilation rates indicate that the rate of decarboxylation of glycine is not directly proportional to the oxygenase activity of Rubisco as is commonly assumed...
  71. ncbi Mixotrophic and photoheterotrophic metabolism in Cyanothece sp. ATCC 51142 under continuous light
    Xueyang Feng
    Department of Energy, Environmental and Chemical Engineering, Washington University, St Louis, MO 63130, USA
    Microbiology 156:2566-74. 2010
    ..Isotopomer analysis also detected that Cyanothece 51142 was able to fix CO(2) via anaplerotic pathways, and to take up glucose and pyruvate for mixotrophic biomass synthesis...
  72. ncbi Automated amino acid side-chain NMR assignment of proteins using (13)C- and (15)N-resolved 3D [ (1)H, (1)H]-NOESY
    Francesco Fiorito
    Institut fur Molekularbiologie und Biophysik, ETH Zurich, CH 8093, Zurich, Switzerland
    J Biomol NMR 42:23-33. 2008
    ....
  73. ncbi Tropane alkaloids from Erythroxylum genus: distribution and compilation of 13C-NMR spectral data
    Steno L Oliveira
    Universidade Federal da Paraiba, Laboratorio de Tecnologia Farmaceutica, Caixa Postal 5009, 58051 970, João Pessoa PB, Brazil
    Chem Biodivers 7:302-26. 2010
    ..This review describes 186 tropane alkaloids in the 35 species of Erythroxylum distributed worldwide. In addition, a compilation of their (13)C-NMR spectral data is presented...
  74. ncbi High temporal resolution tracing of photosynthate carbon from the tree canopy to forest soil microorganisms
    P Högberg
    Department of Forest Ecology and Management, SLU, SE 901 83 Umea, Sweden
    New Phytol 177:220-8. 2008
    ..These observations highlight the close temporal coupling between tree canopy photosynthesis and a significant fraction of soil activity in forests...
  75. ncbi Referencing strategies and techniques in stable isotope ratio analysis
    R A Werner
    Max Planck Institute for Biogeochemistry, P O Box 100164, 07701 Jena, Germany
    Rapid Commun Mass Spectrom 15:501-19. 2001
    ..The implementation of the principle in our laboratory, the monitoring of our measurement quality, the status of the international scales and reference materials and necessary correction procedures are discussed...
  76. ncbi 13C imaging-a new diagnostic platform
    Sven Mansson
    Department of Experimental Research, Malmo University Hospital, 205 02, Malmo, Sweden
    Eur Radiol 16:57-67. 2006
    ..Finally, possible future directions of hyperpolarized 13C MRI are pointed out...
  77. ncbi General method for suppression of diagonal peaks in heteronuclear-edited NOESY spectroscopy
    Jihui Wu
    Department of Biological Sciences and Department of Chemistry, National University of Singapore, 14 Science Drive 4, Singapore 117543
    J Am Chem Soc 126:15018-9. 2004
    ....
  78. ncbi Detection of early response to temozolomide treatment in brain tumors using hyperpolarized 13C MR metabolic imaging
    Ilwoo Park
    Surbeck Laboratory of Advanced Imaging, Department of Radiology and Biomedical Imaging, University of California, San Francisco, California, USA
    J Magn Reson Imaging 33:1284-90. 2011
    ..To demonstrate the feasibility of using DNP hyperpolarized [1-(13)C]-pyruvate to measure early response to temozolomide (TMZ) therapy using an orthotopic human glioblastoma xenograft model...
  79. ncbi Quantitative physiology of Pichia pastoris during glucose-limited high-cell density fed-batch cultivation for recombinant protein production
    Jan Heyland
    Department of Biochemical and Chemical Engineering, TU Dortmund University, Dortmund, Germany
    Biotechnol Bioeng 107:357-68. 2010
    ..The insights into the operation of metabolism during recombinant protein production might guide strain design and fermentation development...
  80. ncbi Minimal model estimation of glucose absorption and insulin sensitivity from oral test: validation with a tracer method
    Chiara Dalla Man
    Department of Information Engineering, University of Padova, Via Gradenigo 6 b, I 35131, Italy
    Am J Physiol Endocrinol Metab 287:E637-43. 2004
    ..86 (P < 0.0001). We conclude that OMM candidates as a reliable tool to measure both the rate of glucose absorption and insulin sensitivity from oral glucose tests without employing tracers...
  81. ncbi Metabolic flux analysis in mammalian cell culture
    Lake Ee Quek
    Australian Institute for Bioengineering and Nanotechnology AIBN, The University of Queensland, Brisbane QLD 4072, Australia
    Metab Eng 12:161-71. 2010
    ..Advances in both modeling and analytical techniques are bringing this challenge within sight...
  82. ncbi Pathway of CH4 formation in anoxic rice field soil and rice roots determined by 13C-stable isotope fractionation
    Ralf Conrad
    Biogeochemistry Department, Max Planck Institut fur terrestrische Mikrobiologie, Marburg, Lahn, Germany
    Chemosphere 47:797-806. 2002
    ..The results demonstrate that there is quite some variability in pathways of CH4 production, and also indicate that isotopic fractionation factors may be different in different habitats and change with time...
  83. ncbi Stable carbon and nitrogen isotope values of bone and teeth reflect weaning age at the Medieval Wharram Percy site, Yorkshire, UK
    M P Richards
    Department of Archaeological Sciences, University of Bradford, Bradford, West Yorkshire BD7 1DP, United Kingdom
    Am J Phys Anthropol 119:205-10. 2002
    ....
  84. ncbi Archaeal community structure and pathway of methane formation on rice roots
    K J Chin
    Max Planck Institut fur terrestrische Mikrobiologie, Karl von Frisch Str, 35043 Marburg, Germany
    Microb Ecol 47:59-67. 2004
    ..Our results demonstrate the parallel change of microbial community structure and function in a complex environment, i.e., the increase of acetoclastic Methanosarcina spp. when high acetate concentrations become available...
  85. pmc Niche width collapse in a resilient top predator following ecosystem fragmentation
    Craig A Layman
    Marine Sciences Program, Department of Biological Sciences, Florida International University, 3000 NE 151st St, North Miami, FL 33181, USA
    Ecol Lett 10:937-44. 2007
    ..Collapsed niche width reflects a homogenization of energy flow pathways to top predators, likely serving to destabilize remnant food webs and render apparently resilient top predators more susceptible to extinction through time...
  86. ncbi Stable isotopes reveal individual variation in migration strategies and habitat preferences in a suite of seabirds during the nonbreeding period
    Richard A Phillips
    British Antarctic Survey, Natural Environment Research Council, High Cross, Cambridge, UK
    Oecologia 160:795-806. 2009
    ..This indicates a difference in C source (distribution), rather than trophic level, and a correspondence between the degree of sexual size dimorphism in Procellariiformes and the level of between-sex niche segregation...
  87. ncbi Intrapopulation variation in gray wolf isotope (delta(15)N and delta(13)C) profiles: implications for the ecology of individuals
    Erin J M Urton
    Department of Biology, University of Saskatchewan, 112 Science Place, Saskatoon, SK, S7N 5E2, Canada
    Oecologia 145:317-26. 2005
    ..Despite social foraging, our results indicate highly variable diets among individuals and we discuss this in terms of individual versus group ecology of boreal wolves...
  88. ncbi Biosynthetic 13C labeling of aromatic side chains in proteins for NMR relaxation measurements
    Kaare Teilum
    Department of Biophysical Chemistry, Lund University, Sweden
    J Am Chem Soc 128:2506-7. 2006
    ..We validated the method by acquiring 13C rotating-frame relaxation dispersion data on the E140Q mutant of the C-terminal domain of calmodulin, which reveal conformational exchange dynamics with a time constant of 71 mus for Y138...
  89. pmc Isotopic values of plants in relation to water availability in the Eastern Mediterranean region
    Gideon Hartman
    Department of Human Evolution, Max Planck Institute for Evolutionary Anthropology, Deutscher Platz 6, 04103 Leipzig, Germany
    Oecologia 162:837-52. 2010
    ..From these results it can be concluded that water availability is the primary factor controlling C and N isotope variability in plant communities in the Eastern Mediterranean...
  90. ncbi Determination of metabolic flux ratios from 13C-experiments and gas chromatography-mass spectrometry data: protocol and principles
    Annik Nanchen
    Institute for Molecular Systems Biology, ETH Zurich, Switzerland
    Methods Mol Biol 358:177-97. 2007
    ..These principles are also implemented in the publicly available software FiatFlux, which directly calculates flux ratios from the mass spectra of amino acids...
  91. pmc c-Myc activates multiple metabolic networks to generate substrates for cell-cycle entry
    F Morrish
    Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Oncogene 28:2485-91. 2009
    ..The pervasive deregulation of this oncogene in human cancers may be explained by its function in directing metabolic networks required for cell proliferation...
  92. pmc Pyruvate carboxylase plays a crucial role in carbon metabolism of extra- and intracellularly replicating Listeria monocytogenes
    Jennifer Schär
    Lehrstuhl fur Mikrobiologie, Biozentrum, Universitat Wurzburg, Wurzburg, Germany
    J Bacteriol 192:1774-84. 2010
    ..monocytogenes. The pycA mutant is also unable to replicate in mammalian cells and exhibits high virulence attenuation in the mouse sepsis model...
  93. pmc A genome-scale metabolic model accurately predicts fluxes in central carbon metabolism under stress conditions
    Thomas C R Williams
    Department of Plant Sciences, University of Oxford, Oxford, United Kingdom
    Plant Physiol 154:311-23. 2010
    ..It is concluded that constraints-based genome-scale modeling can be used to predict flux changes in central carbon metabolism under stress conditions...
  94. ncbi High-resolution direct infusion-based mass spectrometry in combination with whole 13C metabolome isotope labeling allows unambiguous assignment of chemical sum formulas
    Patrick Giavalisco
    Max Planck Institut fur Molekulare Pflanzenphysiologie, 14476 Potsdam Golm, Germany
    Anal Chem 80:9417-25. 2008
    ....
  95. ncbi Correlation of metabolism with tissue carbon and nitrogen turnover rate in small mammals
    Stephen E MacAvoy
    Department of Biology, American University, Hurst Hall 101, 4400 Massachusetts Ave NW, Washington, DC 202 885 2186, USA
    Oecologia 150:190-201. 2006
    ....
  96. ncbi Whole-lake carbon-13 additions reveal terrestrial support of aquatic food webs
    Michael L Pace
    Institute of Ecosystem Studies, Millbrook, New York 12545, USA
    Nature 427:240-3. 2004
    ....
  97. ncbi Stable isotopes as one of nature's ecological recorders
    Jason B West
    Department of Biology, University of Utah, Salt Lake City, UT 84115, USA
    Trends Ecol Evol 21:408-14. 2006
    ..The melding of advances in genetics, biochemical profiling and spatial analysis with those in isotope analyses and modeling sophistication opens the door to an exciting future in ecological research...
  98. ncbi Enhanced sensitivity of DNA- and rRNA-based stable isotope probing by fractionation and quantitative analysis of isopycnic centrifugation gradients
    Tillmann Lueders
    Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, D 35043 Marburg, Germany
    Environ Microbiol 6:73-8. 2004
    ..This is a prerequisite for the fine-scale tracing of microbial populations metabolizing 13C-labelled compounds in natural ecosystems...
  99. pmc Kinetic flux profiling for quantitation of cellular metabolic fluxes
    Jie Yuan
    241 Carl Icahn Laboratory, Department of Chemistry and Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA
    Nat Protoc 3:1328-40. 2008
    ..This protocol is exemplified using E. coli and nitrogen labeling, for which quantitative flux data for approximately 15 metabolites can be obtained over 3 d of work. Applications to adherent mammalian cells are also discussed...
  100. pmc Quantification of compartmented metabolic fluxes in developing soybean embryos by employing biosynthetically directed fractional (13)C labeling, two-dimensional [(13)C, (1)H] nuclear magnetic resonance, and comprehensive isotopomer balancing
    Ganesh Sriram
    Department of Chemical Engineering, Iowa State University, Ames, Iowa 50011, USA
    Plant Physiol 136:3043-57. 2004
    ..The glyoxylate shunt flux was found to be negligible. Such a generic flux analysis tool can serve as a quantitative tool for metabolic studies and phenotype comparisons and can be extended to other plant systems...
  101. ncbi Tobacco aquaporin NtAQP1 is involved in mesophyll conductance to CO2 in vivo
    Jaume Flexas
    Laboratori de Fisiologia Vegetal, Grup de Biologia de les Plantes en Condicions Mediterrànies, Universitat de les Illes Balears Carretera de Valldemossa Km 7 5, 07122 Palma de Mallorca, Balears, Spain
    Plant J 48:427-39. 2006
    ..These results provide evidence for the in vivo involvement of aquaporin NtAQP1 in mesophyll conductance to CO(2)...

Research Grants55

  1. A Compact and Low Cost High-Precision Carbon Isotope Ratio Monitoring Tool for Ro
    Nabil Saad; Fiscal Year: 2009
    ..by coupling a low-cost isotopic CRDS analyzer to a compact Gas Chromatography- Combustion system to measure carbon isotopes of carbon dioxide as the combustion product of individual organic compounds...
  2. MECHANISMS OF OXIDATIVE DAMAGE IN BRAIN
    ROBERT FLOYD; Fiscal Year: 1993
    ..A novel approach of utilizing a combination of alpha-carbon isotopes of the spin-trap PBN (alpha-12CPBN, alpha-13CPBN) administered at specific time points in the development of ..
  3. Aromatic amino acid metabolism in the pathogenesis of kwashiorkor
    Farook Jahoor; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  4. The altered lipid and protein metabolism of pediatric patients with HIV infection
    Farook Jahoor; Fiscal Year: 2010
    ..Knowledge gained from this project may lead to new nutritional therapies to help prevent these conditions. ..
  5. Hyperpolarized High Field NMR Facility For Metabolic Profiling Of Human Disease
    John Kurhanewicz; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  6. MONITORING RADIATION THERAPY OF PROSTATE CANCER BY MRSI
    John Kurhanewicz; Fiscal Year: 2008
    ..This is a translational project intended to provide new biochemical information about prostate cancer before and after radiation therapy and to aid in the translation of this technology into clinical practice. [unreadable] [unreadable]..
  7. MR Based Molecular Imaging of Prostate Cancer
    John Kurhanewicz; Fiscal Year: 2009
    ....
  8. Hyperpolarized 13c NMR Studies of Prostate Cancer (RMI)
    John Kurhanewicz; Fiscal Year: 2008
    ....
  9. Purchase of a 500 MHz Solid State NMR Spectrometer
    Ayyalusamy Ramamoorthy; Fiscal Year: 2007
    ..unreadable] [unreadable] All the major users of the instrument are supported by NIH R01 grants. [unreadable] [unreadable] [unreadable]..
  10. A Novel Diagnostic Test To Detect Vitamin B12 Deficiency
    David Wagner; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  11. TOTAL SYNTHESIS OF AZADIRACHTIN
    K Nicolaou; Fiscal Year: 2008
    ..The proposed work is expected to impact the general areas of pharmaceutical and agricultural research, and infectious diseases in particular, through discoveries in synthetic organic chemistry and chemical biology. ..
  12. MRS STUDIES OF NEUROTRANSMITTER CYCLING IN HUMAN BRAIN
    Douglas Rothman; Fiscal Year: 2007
    ..Although no patient studies are proposed, our goal is for these studies to provide validated methodology and normative values to be used in future studies of neuronal-glial neurotransmitter cycles in development, aging, and disease. ..
  13. Ontogeny of Drug Bioactivation and Idiosyncratic ADRs
    JAMES LEEDER; Fiscal Year: 2007
    ..abstract_text> ..
  14. NONINVASIVE MEASUREMENT OF ENERGY EXPENDITURE IN HUMANS
    Dale Schoeller; Fiscal Year: 2007
    ..These studies will provide important guidelines for the prescription of exercise for the prevention and treatment of obesity. ..
  15. Structure and function of antimicrobial peptides
    Ayyalusamy Ramamoorthy; Fiscal Year: 2008
    ..These peptides have therapeutic potential as antibiotics and have particular importance for cystic fibrosis patients. ..
  16. Novel Technologies for Global Optimization of Magnetic Field Homogeneity
    ROBIN DE GRAAF; Fiscal Year: 2007
    ..Since MFI affects many facet's of in vivo NMR, the proposed techniques will have major impacts on almost all aspects of MRI and MRS. ..
  17. Physiological Signals to MCT Expression
    George Brooks; Fiscal Year: 2008
    ..Aim 3 studies will involve comparisons of the levels of muscle MCT pre-mRNAs, mRNAs and protein levels. We have the tools to achieve the stated aims. ..
  18. The molecular bases of inherited urea cycle disorders and ureagenesis regulation
    Mendel Tuchman; Fiscal Year: 2010
    ....
  19. Cerebral Metabolic Flux Mapping Using Oxygen 17 NMR
    Robin A de Graaf; Fiscal Year: 2010
    ..7 t. ..
  20. SYNTHESIS OF ANTICANCER AGENTS
    K C Nicolaou; Fiscal Year: 2010
    ..abstract_text> ..
  21. Dysfunctional Cortico-Limbic Activity and Connectivity in Bipolar Disorder and Li
    Amit Anand; Fiscal Year: 2010
    ..This study will also establish an experimental paradigm to study brain connectivity which could be used to investigate other disorders. ..
  22. MRS Studies of Brain Metabolic Adaptations in Diabetes
    Douglas Rothman; Fiscal Year: 2008
    ..If MCAs or alternatively medium chain fatty acids can protect against hypoglycemic energy failure they may potentially be administered as a protective therapy for nocturnal hypoglycemic episodes. [unreadable] [unreadable]..
  23. The Effects of Cannabis Use/Abuse on In Vivo Dopamine Function
    WILLIAM FRANKLE; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  24. Ecology of African Highland Malaria
    Guiyun Yan; Fiscal Year: 2007
    ..We anticipate that our results will have broad applicability to malaria prevention and control in Africa. ..
  25. Fully Compensated Dynamic Shim System for In Vivo MRI and MRS
    ROBIN DE GRAAF; Fiscal Year: 2008
    ..Since MFI affects many facets of in vivo NMR, the successful implementation of DSU will have major impacts on almost all aspects of MRI and MRS and can thus be labeled as highly significant. [unreadable] [unreadable] [unreadable]..
  26. Acquisition of a 7T human MR system for the development of ultra high resolution
    Douglas Rothman; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  27. Exercise Substrate Utilization:The Crossover Concept
    George Brooks; Fiscal Year: 2006
    ..Further, we have the auxiliary personnel and facilities to conduct longitudinal training studies ..
  28. PATHOGENESIS OF ANTICONVULSANT HYPERSENSITIVITY SYNDROME
    JAMES LEEDER; Fiscal Year: 2003
    ..It is anticipated that the basic experimental paradigm employed for these studies can also be applied to other idiosyncratic toxicities with suspected drug bioactivation and immune etiologies. ..
  29. A Novel Approach to Carbon-13 Breath Tests
    David Wagner; Fiscal Year: 2002
    ..Our long-term objective is to use RID technology to develop a family of gastrointestinal stable isotope breath tests that are clinically useful and low cost. ..
  30. Intermolecular Multiple-Quantum Coherence Effects in NMR
    Warren Warren; Fiscal Year: 2002
    ....
  31. TOTAL SYNTHESIS OF NATURAL PRODUCTS
    K Nicolaou; Fiscal Year: 2002
    ..3] sigmatropic rearrangement and a radical based ring closure to form the bicyclic skeleton. ..
  32. Quantitative, Dynamic Functional 1H MRS of Human Brain
    ROBIN DE GRAAF; Fiscal Year: 2002
    ..A neurochemical basis of human brain mapping can provide valuable information about functional data comparisons between normal and diseased cerebral tissue. ..
  33. A Breath Test to Detect Bacterial Overgrowth in Elderly
    David Wagner; Fiscal Year: 2003
    ..abstract_text> ..
  34. PHYSIOLOGIC UNDERSTANDING OF FUNCTIONAL MRI
    Fahmeed Hyder; Fiscal Year: 2002
    ....
  35. A Novel Diagnostic Test for Irritable Bowel Syndrome
    David Wagner; Fiscal Year: 2002
    ..abstract_text> ..
  36. 11.74T/210 Millimeter NMR Magnet System/In VivO MRS/MRI
    Douglas Rothman; Fiscal Year: 2002
    ..74T in vivo MRS/MRI system and the application to transgenic mouse and other small animal models for understanding the molecular control of metabolism and physiology in health and disease. ..
  37. TOTAL SYNTHESIS OF APOPTOLIDIN
    K Nicolaou; Fiscal Year: 2004
    ..The proposed work is expected to have significant impact in the area of cancer chemotherapy and should provide enabling technologies and tools for biology and medicine. ..
  38. ISOTOPE RATIO MASS SPECTROMETER
    Dale Schoeller; Fiscal Year: 2006
    ..In addition, this new instrument will provide new capacity in the form of the solids combustion inlet. This expands our potential users group in the area of development of dietary biomarkers. [unreadable] [unreadable]..
  39. Odors maps in olfactory bulb by fMRI
    DEWAN SYED HYDER; Fiscal Year: 2006
    ..Activity of single glomeruli can help elucidate mechanisms of olfactory processing. ..
  40. Genes controlling heart function in a hibernating mammal
    Matthew Andrews; Fiscal Year: 2005
    ..abstract_text> ..
  41. Quantitative, Dynamic Functional 1H MRS of Human Brain
    ROBIN DE GRAAF; Fiscal Year: 2005
    ..A neurochemical basis of human brain mapping can provide valuable information about functional data comparisons between normal and diseased cerebral tissue. ..
  42. Radio Frequency Impedance Mapping for Medical Imaging
    AARON GRANT; Fiscal Year: 2005
    ..Furthermore, RFIM is highly non-invasive and RFIM imaging systems are likely to be both inexpensive and easily portable. ..
  43. Enabling Technologies for Combinatorial Chemistry
    K Nicolaou; Fiscal Year: 2005
    ..abstract_text> ..
  44. Two-Photon Absorption Imaging by Laser Pulse Shaping
    Warren Warren; Fiscal Year: 2004
    ..abstract_text> ..
  45. A NOVEL METHOD TO DETECT HELICOBACTER PYLORI IN CHILDREN
    David Wagner; Fiscal Year: 2004
    ..The simplicity of the Ez-HBT(TM) including no test meal, a tablet that can be dissolved in a glass of water and a single traditional venipuncture makes it an ideal and cost effective tool for identifying H. pylori infection in children. ..
  46. KETOGENIC DIET AND BRAIN AMINO ACID METABOLISM
    Marc Yudkoff; Fiscal Year: 2002
    ....
  47. GENETIC PATHWAYS TOWARD GLIOMAGENESIS
    Elizabeth Maher; Fiscal Year: 2003
    ..Aim 4: To identify genes that cooperate with known oncogenes and tumor suppressors in the development and/or progression of malignant gliomas using a well-established retroviral insertional approach. ..
  48. The Neuroanatomy of Antipsychotic Drug Action
    WILLIAM FRANKLE; Fiscal Year: 2008
    ..abstract_text> ..
  49. Development of PET radioligands for cerebral cannabinoid receptor (CB1)
    ANDREW HORTI; Fiscal Year: 2008
    ....
  50. SCALING THE REINFORCING VALUE OF CIGARETTE SMOKE
    Jed Rose; Fiscal Year: 2003
    ..The results will lead to a better understanding of the role of pharmacologic and sensory/behavioral cues in nicotine addiction and smoking cessation. ..
  51. The Influence of Static Magnetic Fields on Brain tissue
    Andrzej Wieraszko; Fiscal Year: 2003
    ..The cell excitability, synaptic efficiency and action potentials recorded from the slices exposed to SMF will be studied. The directions of alternative and future research are delineated. ..
  52. Quantitative Simultaneous Dual Isotope SPECT
    Georges El Fakhri; Fiscal Year: 2008
    ..We expect that our approach will make quantitative simultaneous dualisotope imaging clinically feasible, and lead to earlier diagnosis and better monitoring of PD and to improved evaluation of suspected acute cardiac ischemia. ..
  53. MR SPECTROSCOPIC IMAGING IN ALZHEIMER'S DISEASE
    Daniel Spielman; Fiscal Year: 2003
    ..The cognitive correlates of the regional MRSI data will be examined by testing specific brain-behavior relationships through double dissociation models. ..
  54. MAGNETIC RESONANCE SPECTROSCOPIC NEOPLASM IMAGING
    Daniel Spielman; Fiscal Year: 2003
    ..abstract_text> ..
  55. Neurochemical Correlates of Pathology in Spinocerebellar Ataxias
    GULIN OZ; Fiscal Year: 2008
    ..Successful application of such treatments is expected to reduce the burden caused by these diseases on individuals, their families and society. [unreadable] [unreadable] [unreadable]..