ice

Summary

Top Publications

  1. ncbi The genetic legacy of the Quaternary ice ages
    G Hewitt
    School of Biological Sciences, University of East Anglia, Norwich, UK
    Nature 405:907-13. 2000
  2. pmc Movies of ice-embedded particles enhance resolution in electron cryo-microscopy
    Melody G Campbell
    National Resource for Automated Molecular Microscopy, Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Structure 20:1823-8. 2012
  3. ncbi Antarctic Sea ice--a habitat for extremophiles
    D N Thomas
    School of Ocean Sciences, University of Wales Bangor, Menai Bridge, Anglesey, UK, LL59 5EY
    Science 295:641-4. 2002
  4. pmc Bacteria in the leaf ecosystem with emphasis on Pseudomonas syringae-a pathogen, ice nucleus, and epiphyte
    S S Hirano
    Department of Plant Pathology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Microbiol Mol Biol Rev 64:624-53. 2000
  5. pmc Climate change winners: receding ice fields facilitate colony expansion and altered dynamics in an Adélie penguin metapopulation
    Michelle A Larue
    Department of Earth Sciences, Polar Geospatial Center, University of Minnesota, St Paul, Minnesota, United States of America
    PLoS ONE 8:e60568. 2013
  6. ncbi Freezing as a path to build complex composites
    Sylvain Deville
    Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
    Science 311:515-8. 2006
  7. ncbi The relevance of ice crystal formation for the cryopreservation of tissues and organs
    David E Pegg
    Department of Biology, University of York, YO10 5YW, UK
    Cryobiology 60:S36-44. 2010
  8. ncbi Sea ice-associated diet change increases the levels of chlorinated and brominated contaminants in polar bears
    Melissa A McKinney
    Wildlife Toxicology and Disease Program, Science and Technology Branch, Environment Canada, Ottawa, Ontario KJA 0H3, Canada
    Environ Sci Technol 43:4334-9. 2009
  9. ncbi Remarkably low temperature optima for extracellular enzyme activity from Arctic bacteria and sea ice
    A L Huston
    School of Oceanography, University of Washington, Seattle 98195, USA
    Environ Microbiol 2:383-8. 2000
  10. ncbi Ocean circulation off east Antarctica affects ecosystem structure and sea-ice extent
    S Nicol
    Department of the Environment and Heritage, Channel Highway, Kingston, Tasmania, Australia
    Nature 406:504-7. 2000

Detail Information

Publications310 found, 100 shown here

  1. ncbi The genetic legacy of the Quaternary ice ages
    G Hewitt
    School of Biological Sciences, University of East Anglia, Norwich, UK
    Nature 405:907-13. 2000
    Global climate has fluctuated greatly during the past three million years, leading to the recent major ice ages...
  2. pmc Movies of ice-embedded particles enhance resolution in electron cryo-microscopy
    Melody G Campbell
    National Resource for Automated Molecular Microscopy, Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Structure 20:1823-8. 2012
    ..Using movies in this way is generally applicable to all cryo-EM samples and should also improve the performance of midrange electron microscopes that may have limited mechanical stability and beam coherence...
  3. ncbi Antarctic Sea ice--a habitat for extremophiles
    D N Thomas
    School of Ocean Sciences, University of Wales Bangor, Menai Bridge, Anglesey, UK, LL59 5EY
    Science 295:641-4. 2002
    The pack ice of Earth's polar oceans appears to be frozen white desert, devoid of life...
  4. pmc Bacteria in the leaf ecosystem with emphasis on Pseudomonas syringae-a pathogen, ice nucleus, and epiphyte
    S S Hirano
    Department of Plant Pathology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Microbiol Mol Biol Rev 64:624-53. 2000
    ..communities, with emphasis on Pseudomonas syringae-a species that participates in leaf ecosystems as a pathogen, ice nucleus, and epiphyte...
  5. pmc Climate change winners: receding ice fields facilitate colony expansion and altered dynamics in an Adélie penguin metapopulation
    Michelle A Larue
    Department of Earth Sciences, Polar Geospatial Center, University of Minnesota, St Paul, Minnesota, United States of America
    PLoS ONE 8:e60568. 2013
    ..Although the Ross Sea is likely to be the last ocean with an intact ecosystem, the recent retreat of ice fields at Beaufort that resulted in increased breeding habitat exemplifies a process that has been underway in the ..
  6. ncbi Freezing as a path to build complex composites
    Sylvain Deville
    Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
    Science 311:515-8. 2006
    ..We demonstrate how the physics of ice formation can be used to develop sophisticated porous and layered-hybrid materials, including artificial bone, ..
  7. ncbi The relevance of ice crystal formation for the cryopreservation of tissues and organs
    David E Pegg
    Department of Biology, University of York, YO10 5YW, UK
    Cryobiology 60:S36-44. 2010
    This paper discusses the role of ice crystal formation in causing or contributing to the difficulties that have been encountered in attempts to develop effective methods for the cryopreservation of some tissues and all organs...
  8. ncbi Sea ice-associated diet change increases the levels of chlorinated and brominated contaminants in polar bears
    Melissa A McKinney
    Wildlife Toxicology and Disease Program, Science and Technology Branch, Environment Canada, Ottawa, Ontario KJA 0H3, Canada
    Environ Sci Technol 43:4334-9. 2009
    ..Differences in timing of the annual sea ice breakup explained a significant proportion of the diet variation among years...
  9. ncbi Remarkably low temperature optima for extracellular enzyme activity from Arctic bacteria and sea ice
    A L Huston
    School of Oceanography, University of Washington, Seattle 98195, USA
    Environ Microbiol 2:383-8. 2000
    ..extracellular enzymatic activity (EEA) with remarkably low temperature optima (15 degrees C) was documented in sea-ice samples...
  10. ncbi Ocean circulation off east Antarctica affects ecosystem structure and sea-ice extent
    S Nicol
    Department of the Environment and Heritage, Channel Highway, Kingston, Tasmania, Australia
    Nature 406:504-7. 2000
    Sea ice and oceanic boundaries have a dominant effect in structuring Antarctic marine ecosystems...
  11. ncbi Properties, potentials, and prospects of antifreeze proteins
    S Venketesh
    Department of Biochemistry, Indian Institute of Science, Bangalore, India
    Crit Rev Biotechnol 28:57-82. 2008
    ..Mutational studies have indicated the importance of hydrophobic residues in ice binding...
  12. ncbi Poles apart: biodiversity and biogeography of sea ice bacteria
    J T Staley
    Department of Microbiology 357242, University of Washington, Seattle 98195, USA
    Annu Rev Microbiol 53:189-215. 1999
    ..This review discusses sea ice bacteriology as a test case for examining bacterial diversity and biogeography...
  13. pmc Diversity and structure of bacterial communities in Arctic versus Antarctic pack ice
    Robin Brinkmeyer
    Alfred Wegener Institute for Polar and Marine Research, Bremerhaven, Germany
    Appl Environ Microbiol 69:6610-9. 2003
    A comprehensive assessment of bacterial diversity and community composition in arctic and antarctic pack ice was conducted through cultivation and cultivation-independent molecular techniques...
  14. ncbi Ice-binding proteins from enoki and shiitake mushrooms
    James A Raymond
    School of Life Sciences, University of Nevada, Las Vegas, NV 89154, USA
    Cryobiology 58:151-6. 2009
    Fungi have developed a variety of mechanisms for tolerating cold, including production of proteins that bind to ice, as shown by their ability to slightly lower the freezing point...
  15. ncbi Antifreeze protein from freeze-tolerant grass has a beta-roll fold with an irregularly structured ice-binding site
    Adam J Middleton
    Department of Biomedical and Molecular Sciences, Queen s University, Kingston, Ontario, Canada K7L 3N6
    J Mol Biol 416:713-24. 2012
    The grass Lolium perenne produces an ice-binding protein (LpIBP) that helps this perennial tolerate freezing by inhibiting the recrystallization of ice...
  16. ncbi Cartilage tissue engineering using funnel-like collagen sponges prepared with embossing ice particulate templates
    Hongxu Lu
    Biomaterials Center, National Institute for Materials Science, 1 1 Namiki, Tsukuba, Ibaraki 305 0044, Japan
    Biomaterials 31:5825-35. 2010
    ..funnel-like collagen sponges with open surface pore structures by a freeze-drying method that used embossing ice particulates as a template...
  17. ncbi Ice recrystallization inhibition proteins of perennial ryegrass enhance freezing tolerance
    Chunzhen Zhang
    Department of Horticulture, Iowa State University, Ames, IA 50011 1100, USA
    Planta 232:155-64. 2010
    b>Ice recrystallization inhibition (IRI) proteins are thought to play an important role in conferring freezing tolerance in plants...
  18. pmc Subglacial Lake Vostok (Antarctica) accretion ice contains a diverse set of sequences from aquatic, marine and sediment-inhabiting bacteria and eukarya
    Yury M Shtarkman
    Department of Biological Sciences, Bowling Green State University, Bowling Green, Ohio, United States of America
    PLoS ONE 8:e67221. 2013
    Lake Vostok, the 7(th) largest (by volume) and 4(th) deepest lake on Earth, is covered by more than 3,700 m of ice, making it the largest subglacial lake known...
  19. ncbi Sea ice cover and its influence on Adélie Penguin reproductive performance
    Louise Emmerson
    Australian Antarctic Division, Department of the Environment, Water, Heritage and The Arts, Channel Highway, Kingston, 7050 Tasmania, Australia
    Ecology 89:2096-102. 2008
    The relationship between Adélie Penguins (Pygoscelis adeliae) and ice is well established, with sea ice influencing penguin populations through a variety of processes operating at different spatial and temporal scales...
  20. ncbi High latitude changes in ice dynamics and their impact on polar marine ecosystems
    Mark A Moline
    Biological Sciences Department and Center for Coastal Marine Sciences, California Polytechnic State University, San Luis Obispo, CA 93407, USA
    Ann N Y Acad Sci 1134:267-319. 2008
    ..Basin and along the Antarctic Peninsula, with significant decreases in the extent and seasonal duration of sea ice. Rapid retreat of glaciers and disintegration of ice sheets have also been documented...
  21. pmc Phylogenetic and physiological diversity of microorganisms isolated from a deep greenland glacier ice core
    V I Miteva
    NASA Astrobiology Institute and Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Appl Environ Microbiol 70:202-13. 2004
    We studied a sample from the GISP 2 (Greenland Ice Sheet Project) ice core to determine the diversity and survival of microorganisms trapped in the ice at least 120,000 years ago...
  22. pmc Superheating of ice crystals in antifreeze protein solutions
    Yeliz Celik
    Department of Physics and Astronomy, Ohio University, Athens, OH 45701, USA
    Proc Natl Acad Sci U S A 107:5423-8. 2010
    It has been argued that for antifreeze proteins (AFPs) to stop ice crystal growth, they must irreversibly bind to the ice surface...
  23. pmc Microfluidic experiments reveal that antifreeze proteins bound to ice crystals suffice to prevent their growth
    Yeliz Celik
    Department of Physics and Astronomy, Ohio University, Athens, OH 45701, USA
    Proc Natl Acad Sci U S A 110:1309-14. 2013
    Antifreeze proteins (AFPs) are a subset of ice-binding proteins that control ice crystal growth...
  24. pmc Microbial life at -13 °C in the brine of an ice-sealed Antarctic lake
    Alison E Murray
    Division of Earth and Ecosystem Sciences, Desert Research Institute, Reno, NV 89512, USA
    Proc Natl Acad Sci U S A 109:20626-31. 2012
    The permanent ice cover of Lake Vida (Antarctica) encapsulates an extreme cryogenic brine ecosystem (-13 °C; salinity, 200). This aphotic ecosystem is anoxic and consists of a slightly acidic (pH 6.2) sodium chloride-dominated brine...
  25. ncbi Freeze tolerance, supercooling points and ice formation: comparative studies on the subzero temperature survival of limno-terrestrial tardigrades
    S Hengherr
    Universitat Stuttgart, Biological Institute, Department of Zoology, Pfaffenwaldring 57, 70569 Stuttgart, Germany
    J Exp Biol 212:802-7. 2009
    ..fed and cold acclimatized individuals showed no intraspecific significant differences in supercooling points and ice formation...
  26. ncbi Ubiquity of biological ice nucleators in snowfall
    Brent C Christner
    Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
    Science 319:1214. 2008
    Despite the integral role of ice nucleators (IN) in atmospheric processes leading to precipitation, their sources and distributions have not been well established...
  27. ncbi Antarctic krill under sea ice: elevated abundance in a narrow band just south of ice edge
    Andrew S Brierley
    British Antarctic Survey, High Cross, Madingley Road, Cambridge CB3 0ET, UK
    Science 295:1890-2. 2002
    We surveyed Antarctic krill (Euphausia superba) under sea ice using the autonomous underwater vehicle Autosub-2. Krill were concentrated within a band under ice between 1 and 13 kilometers south of the ice edge...
  28. pmc Ice-age endurance: DNA evidence of a white spruce refugium in Alaska
    Lynn L Anderson
    Program in Ecology and Evolutionary Biology and Departments of Plant Biology, Geology, and Animal Biology, University of Illinois, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 103:12447-50. 2006
    ..arguing against the possibility that this species migrated into the region from areas south of the Laurentide Ice Sheet after the end of the last glaciation...
  29. pmc Structure and function of antifreeze proteins
    Peter L Davies
    Department of Biochemistry, Queen s University, Kingston, Ontario, Canada K7L 3N6
    Philos Trans R Soc Lond B Biol Sci 357:927-35. 2002
    ..For each of these structures, the ice-binding site of the AFP has been defined by site-directed mutagenesis, and ice etching has indicated that the ice ..
  30. ncbi Nunatak survival of the high Alpine plant Eritrichium nanum (L.) Gaudin in the central Alps during the ice ages
    I Stehlik
    Institute of Systematic Botany, University of Zurich, Zollikerstrasse 107, Ch 8008 Zurich, Switzerland
    Mol Ecol 11:2027-36. 2002
    ..This haplotype is hypothesized to be genetically identical to a Tertiary Alpine colonizing ancestor, whose distribution was secondarily fragmented and infiltrated by derived haplotypes originating through local mutations...
  31. ncbi Zernike phase contrast electron microscopy of ice-embedded influenza A virus
    Masashi Yamaguchi
    Medical Mycology Research Center, Chiba University, 1 8 1 Inohana, Chuo Ku, Chiba 260 8673, Japan
    J Struct Biol 162:271-6. 2008
    ..is a powerful tool for resolving the ultrastructure of viruses, because it enables high-contrast images of ice-embedded particles free of contrast transfer function artifacts that can be a problem in conventional cryo-electron ..
  32. pmc Anchored clathrate waters bind antifreeze proteins to ice
    Christopher P Garnham
    Department of Biochemistry, Queen s University, Kingston, ON, Canada K7L 3N6
    Proc Natl Acad Sci U S A 108:7363-7. 2011
    The mechanism by which antifreeze proteins (AFPs) irreversibly bind to ice has not yet been resolved. The ice-binding site of an AFP is relatively hydrophobic, but also contains many potential hydrogen bond donors/acceptors...
  33. ncbi Identification of the ice-binding face of antifreeze protein from Tenebrio molitor
    Christopher B Marshall
    Department of Biochemistry, Queen s University, Kingston, Ontario, Canada K7L 3N6
    FEBS Lett 529:261-7. 2002
    ..antifreeze protein with one surface comprised of an array of Thr residues that putatively interacts with ice. In order to use mutagenesis to identify the ice-binding face, we have selected an isoform that folds well and is ..
  34. ncbi Functional evaluation of candidate ice structuring proteins using cell-free expression systems
    A K Brödel
    Fraunhofer Institute for Biomedical Engineering IBMT, Branch Potsdam Golm, Am Muhlenberg 13, 14476 Potsdam, Germany
    J Biotechnol 163:301-10. 2013
    b>Ice structuring proteins (ISPs) protect organisms from damage or death by freezing. They depress the non-equilibrium freezing point of water and prevent recrystallization, probably by binding to the surface of ice crystals...
  35. ncbi Controlled ice nucleation in cryopreservation--a review
    G John Morris
    Asymptote Ltd, St John s Innovation Centre, Cowley Road, Cambridge, UK
    Cryobiology 66:85-92. 2013
    We review here for the first time, the literature on control of ice nucleation in cryopreservation. Water and aqueous solutions have a tendency to undercool before ice nucleation occurs...
  36. ncbi Survival and breeding of polar bears in the southern Beaufort Sea in relation to sea ice
    Eric V Regehr
    US Geological Survey, Alaska Science Center, 4210 University Dr, Anchorage, AK 99508, USA
    J Anim Ecol 79:117-27. 2010
    1. Observed and predicted declines in Arctic sea ice have raised concerns about marine mammals...
  37. pmc Problems in obtaining perfect images by single-particle electron cryomicroscopy of biological structures in amorphous ice
    Richard Henderson
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, UK
    Microscopy (Oxf) 62:43-50. 2013
    ..together with simulations of single-particle electron cryomicroscopy images of biological assemblies in ice demonstrate that atomic structures should be obtainable from images of a few thousand asymmetric units, provided ..
  38. ncbi Hydration index--a better parameter for explaining small molecule hydration in inhibition of ice recrystallization
    Roger Y Tam
    Department of Chemistry, D Iorio Hall, 10 Marie Curie, University of Ottawa, Ottawa, Ontario, Canada, K1N 6N5
    J Am Chem Soc 130:17494-501. 2008
    Several simple mono- and disaccharides have been assessed for their ability to inhibit ice recrystallization. Two carbohydrates were found to be effective recrystallization inhibitors...
  39. ncbi Electromelting of confined monolayer ice
    Hu Qiu
    State Key Laboratory of Mechanics and Control of Mechanical Structures, Institute of Nano Science, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China
    Phys Rev Lett 110:195701. 2013
    ..view that electric fields promote water freezing, here we show by molecular dynamics simulations that monolayer ice confined between two parallel plates can melt into liquid water under a perpendicularly applied electric field...
  40. ncbi Diversity and cold-active hydrolytic enzymes of culturable bacteria associated with Arctic sea ice, Spitzbergen
    Tatiana Groudieva
    Institute of Technical Microbiology, Technical University Hamburg Harburg, Kasernenstr 12, 21073 Hamburg, Germany
    Extremophiles 8:475-88. 2004
    The diversity of culturable bacteria associated with sea ice from four permanently cold fjords of Spitzbergen, Arctic Ocean, was investigated...
  41. ncbi Hyperactive antifreeze protein from fish contains multiple ice-binding sites
    Laurie A Graham
    Department of Biochemistry and Protein Function Discovery Group, Queen s University, Kingston, Ontario, Canada K7L 3N6
    Biochemistry 47:2051-63. 2008
    ..of nonhomologous types of AFPs, diverse in both sequence and structure, which share the function of binding to ice and inhibiting its growth...
  42. doi The importance of hydration for inhibiting ice recrystallization with C-linked antifreeze glycoproteins
    Pawel Czechura
    University of Ottawa, Department of Chemistry, D Iorio Hall, 10 Marie Curie, Ottawa, ON, Canada K1N 6N5
    J Am Chem Soc 130:2928-9. 2008
  43. ncbi Cryopreservation of human ovarian tissue: effect of spontaneous and initiated ice formation
    Vladimir Isachenko
    Department of Gynecological Endocrinology and Reproductive Medicine, University of Bonn, Sigmund Freud Str 25, 53105 Bonn, Germany
    Reprod Biomed Online 16:336-45. 2008
    ..compared conventional freezing of human ovarian tissue using either spontaneous or initiated ('seeded') ice formation. Biopsies of ovarian tissue were obtained from women with indications for chemotherapy or radiotherapy...
  44. ncbi Effect of annealing time of an ice crystal on the activity of type III antifreeze protein
    Manabu Takamichi
    Functional Protein Research Group, Research Institute of Genome Based Biofactory, National Institute of Advanced Industrial Science and Technology, Toyohira, Sapporo, Japan
    FEBS J 274:6469-76. 2007
    Antifreeze proteins (AFPs) possess a unique ability to bind to a seed ice crystal to inhibit its growth. The strength of this binding has been evaluated by thermal hysteresis (TH)...
  45. ncbi Sea ice phenology and timing of primary production pulses in the Arctic Ocean
    Rubao Ji
    Department of Biology, Woods Hole Oceanographic Institution, MS 33, Redfield 2 14, Woods Hole, MA 02543, USA
    Glob Chang Biol 19:734-41. 2013
    ..Climate warming causes shrinking ice coverage and earlier ice retreat in the Arctic, which is likely to change the timing of primary production...
  46. pmc Crystal structure of an insect antifreeze protein and its implications for ice binding
    Aaron Hakim
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    J Biol Chem 288:12295-304. 2013
    Antifreeze proteins (AFPs) help some organisms resist freezing by binding to ice crystals and inhibiting their growth. The molecular basis for how these proteins recognize and bind ice is not well understood...
  47. ncbi First live birth in germany after re-transplantation of cryopreserved ovarian tissue: original device for initiation of ice formation
    Vladimir Isachenko
    Universitotsfrauenklinik Cologne, Cologne, Germany
    Clin Lab 58:933-8. 2012
    ..The problem of post-cancer infertility is of significant concern. The cryopreservation of ovarian tissue before cancer therapy with retransplantation after convalescence is the key to solving this problem...
  48. ncbi Molecular characterization and origin of novel bipartite cold-regulated ice recrystallization inhibition proteins from cereals
    Karine Tremblay
    Universite du Quebec a Montreal, Departement des Sciences Biologiques, CP 8888, Succursale Centre Ville, Montreal, Quebec H3C 3P8, Canada
    Plant Cell Physiol 46:884-91. 2005
    ..Among these are two genes named TaIRI-1 and TaIRI-2 (Triticum aestivum ice recrystallization inhibition) that are up-regulated during cold acclimation in freezing-tolerant species...
  49. pmc Microbial communities in the subglacial waters of the Vatnajökull ice cap, Iceland
    Viggo Thor Marteinsson
    Matis, Department of Food Safety, Environment, and Genetics, Reykjavik, Iceland
    ISME J 7:427-37. 2013
    Subglacial lakes beneath the Vatnajökull ice cap in Iceland host endemic communities of microorganisms adapted to cold, dark and nutrient-poor waters, but the mechanisms by which these microbes disseminate under the ice and colonize ..
  50. pmc Structural basis for antifreeze activity of ice-binding protein from arctic yeast
    Jun Hyuck Lee
    Division of Polar Life Sciences, Korea Polar Research Institute, Incheon 406 840, Republic of Korea
    J Biol Chem 287:11460-8. 2012
    Arctic yeast Leucosporidium sp. produces a glycosylated ice-binding protein (LeIBP) with a molecular mass of ∼25 kDa, which can lower the freezing point below the melting point once it binds to ice...
  51. ncbi Why superhydrophobic surfaces are not always icephobic
    Michael Nosonovsky
    College of Engineering and Applied Science, University of Wisconsin Milwaukee, Milwaukee, Wisconsin 53211, USA
    ACS Nano 6:8488-91. 2012
    We discuss mechanical forces that act upon a water droplet and a piece of ice on a rough solid surface and the difference between dewetting and ice fracture...
  52. ncbi Ice slurry on outdoor running performance in heat
    Z W Yeo
    Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore
    Int J Sports Med 33:859-66. 2012
    The efficacy of ingestion of ice slurry on actual outdoor endurance performance is unknown. This study aimed to investigate ice slurry ingestion as a cooling intervention before a 10 km outdoor running time-trial...
  53. ncbi Mechanism of supercooled droplet freezing on surfaces
    Stefan Jung
    Laboratory of Thermodynamics in Emerging Technologies, Mechanical and Process Engineering Department, ETH Zurich, 8092 Zurich, Switzerland
    Nat Commun 3:615. 2012
    Understanding ice formation from supercooled water on surfaces is a problem of fundamental importance and general utility. Superhydrophobic surfaces promise to have remarkable 'icephobicity' and low ice adhesion...
  54. ncbi High pressure partially ionic phase of water ice
    Yanchao Wang
    State Key Laboratory of Superhard Materials, Jilin University, Changchun 130012, China
    Nat Commun 2:563. 2011
    Water ice dissociates into a superionic solid at high temperature (>2,000 K) and pressure, where oxygen forms the lattice, but hydrogen diffuses completely...
  55. ncbi Glacial refugia of limber pine (Pinus flexilis James) inferred from the population structure of mitochondrial DNA
    J B Mitton
    Department of Environmental, Population and Organismic Biology, University of Colorado, Boulder, CO 80309, USA
    Mol Ecol 9:91-7. 2000
    ..The majority of the mtDNA haplotypes are restricted to minor portions of the geographical range. The data are consistent with mtDNA differentiation in seven glacial refugia, followed by dispersal out of those refugia...
  56. ncbi Islands in the ice: detecting past vegetation on Greenlandic nunataks using historical records and sedimentary ancient DNA meta-barcoding
    Tina Jørgensen
    Centre for GeoGenetics, Natural History Museum of Denmark, University of Copenhagen, Copenhagen, Denmark
    Mol Ecol 21:1980-8. 2012
    Nunataks are isolated bedrocks protruding through ice sheets. They vary in age, but represent island environments in 'oceans' of ice through which organism dispersals and replacements can be studied over time. The J.A.D...
  57. ncbi C-linked antifreeze glycoprotein (C-AFGP) analogues as novel cryoprotectants
    Mathieu Leclère
    Department of Chemistry, Regenerative Medicine Program, University of Ottawa, Ontario, Canada
    Bioconjug Chem 22:1804-10. 2011
    ..Cell damage from ice recrystallization during freeze-thawing is one cause of decreased viability after cryopreservation...
  58. ncbi Disaster-related prenatal maternal stress influences birth outcomes: project Ice Storm
    Kelsey N Dancause
    Douglas Hospital Research Center, 6875 LaSalle Blvd, Verdun QC, Canada H4H 1R3
    Early Hum Dev 87:813-20. 2011
    ..Previous research suggests that prenatal maternal stress (PNMS) impacts birth outcomes, but many human studies cannot distinguish between the effects of different types of stressors or examine effects of exposure timing on outcomes...
  59. pmc New insights into ice growth and melting modifications by antifreeze proteins
    Maya Bar-Dolev
    The Robert H Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
    J R Soc Interface 9:3249-59. 2012
    Antifreeze proteins (AFPs) evolved in many organisms, allowing them to survive in cold climates by controlling ice crystal growth...
  60. ncbi Inhibiting ice recrystallization and optimization of cell viability after cryopreservation
    Jennifer L Chaytor
    Department of Chemistry, University of Ottawa, D Iorio Hall, 10 Marie Curie, Ottawa, ON, Canada K1N 6N5
    Glycobiology 22:123-33. 2012
    The ice recrystallization inhibition activity of various mono- and disaccharides has been correlated with their ability to cryopreserve human cell lines at various concentrations...
  61. pmc Ice-binding site of snow mold fungus antifreeze protein deviates from structural regularity and high conservation
    Hidemasa Kondo
    Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Sapporo 062 8517, Japan
    Proc Natl Acad Sci U S A 109:9360-5. 2012
    ..AFPs that protect freeze-intolerant fish and insects from internal ice growth bind to ice using a regular array of well-conserved residues/motifs...
  62. ncbi Photosynthetic energy conversion under extreme conditions--II: the significance of lipids under light limited growth in Antarctic sea ice diatoms
    Thomas Mock
    Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, D 27570 Bremerhaven, Germany
    Phytochemistry 61:53-60. 2002
    Low photosynthetic active radiation is a strong determinant in the development and growth of sea ice algae. The algae appear to have universal mechanisms to overcome light limitation...
  63. pmc Fluorescence microscopy evidence for quasi-permanent attachment of antifreeze proteins to ice surfaces
    Natalya Pertaya
    Department of Physics and Astronomy, Ohio University, Athens, Ohio, USA
    Biophys J 92:3663-73. 2007
    ..Many organisms are protected from freezing by the presence of extracellular antifreeze proteins (AFPs), which bind to ice, modify its morphology, and prevent its further growth...
  64. ncbi Ice slurry ingestion increases core temperature capacity and running time in the heat
    Rodney Siegel
    School of Exercise, Biomedical and Health Sciences, Edith Cowan University, Joondalup, WA, Australia
    Med Sci Sports Exerc 42:717-25. 2010
    To investigate the effect of ice slurry ingestion on thermoregulatory responses and submaximal running time in the heat.
  65. ncbi Understanding the mechanism of ice binding by type III antifreeze proteins
    A A Antson
    Structural Biology Laboratory Department of Chemistry, University of York, Heslington, York Y01 5DD, UK
    J Mol Biol 305:875-89. 2001
    Type III antifreeze proteins (AFPs) are present in the body fluids of some polar fishes where they inhibit ice growth at subzero temperatures...
  66. ncbi Liquid-infused nanostructured surfaces with extreme anti-ice and anti-frost performance
    Philseok Kim
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts 02138, USA
    ACS Nano 6:6569-77. 2012
    b>Ice-repellent coatings can have significant impact on global energy savings and improving safety in many infrastructures, transportation, and cooling systems...
  67. ncbi An ice-binding protein from an Antarctic sea ice bacterium
    James A Raymond
    School of Life Sciences, University of Nevada, Las Vegas, NV, USA
    FEMS Microbiol Ecol 61:214-21. 2007
    An Antarctic sea ice bacterium of the Gram-negative genus Colwellia, strain SLW05, produces an extracellular substance that changes the morphology of growing ice...
  68. pmc The association of Antarctic krill Euphausia superba with the under-ice habitat
    Hauke Flores
    Institute for Marine Resources and Ecosystem Studies, Texel, The Netherlands
    PLoS ONE 7:e31775. 2012
    The association of Antarctic krill Euphausia superba with the under-ice habitat was investigated in the Lazarev Sea (Southern Ocean) during austral summer, autumn and winter...
  69. pmc Ice shaping properties, similar to that of antifreeze proteins, of a zirconium acetate complex
    Sylvain Deville
    Laboratoire de Synthèse et Fonctionnalisation des Céramiques, UMR3080 CNRS Saint Gobain, Cavaillon, France
    PLoS ONE 6:e26474. 2011
    The control of the growth morphologies of ice crystals is a critical issue in fields as diverse as biomineralization, medicine, biology, civil or food engineering...
  70. pmc Metabolomics as a powerful tool for molecular quality assessment of the fish Sparus aurata
    Gianfranco Picone
    Department of Food Science, University of Bologna, Piazza Goidanich 60, 47023 Cesena, Italy
    Nutrients 3:212-27. 2011
    ..and substantially maintain the same differences in their molecular profiles after sixteen days of storage under ice. The fish metabolic profiles are studied by top-down chemometric analysis...
  71. ncbi Design and synthesis of antifreeze glycoproteins and mimics
    James Garner
    School of Chemistry, The University of New South Wales, Sydney, NSW 2052, Australia
    Chembiochem 11:2489-98. 2010
    ..antifreezes that have potential applications in many areas of medicine, agriculture and industry in which ice crystal growth is damaging...
  72. ncbi Predictive model for ice formation on superhydrophobic surfaces
    Vaibhav Bahadur
    School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts 02138, USA
    Langmuir 27:14143-50. 2011
    The prevention and control of ice accumulation has important applications in aviation, building construction, and energy conversion devices...
  73. ncbi Type I shorthorn sculpin antifreeze protein: recombinant synthesis, solution conformation, and ice growth inhibition studies
    Kayesh Fairley
    School of Chemistry and the Department of Biochemistry, University of Sydney, New South Wales 2006, Australia
    J Biol Chem 277:24073-80. 2002
    A number of structurally diverse classes of "antifreeze" proteins that allow fish to survive in sub-zero ice-laden waters have been isolated from the blood plasma of cold water teleosts...
  74. ncbi Refined solution structure of type III antifreeze protein: hydrophobic groups may be involved in the energetics of the protein-ice interaction
    F D Sonnichsen
    Department of Physiology and Biophysics, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, Ohio 44106 4970, USA
    Structure 4:1325-37. 1996
    ..of blood and body fluids below that of the surrounding sea water by binding to and inhibiting the growth of seed ice crystals...
  75. ncbi Simulated distributions of Baltic Sea-ice in warming climate and consequences for the winter habitat of the Baltic ringed seal
    H E Markus Meier
    Rossby Centre, Swedish Meteorological and Hydrological Institute, Norrkoping
    Ambio 33:249-56. 2004
    Sea-ice in the Baltic Sea in present and future climates is investigated. The Rossby Centre Regional Atmosphere-Ocean model was used to perform a set of 30-year-long time slice experiments...
  76. pmc Direct visualization of spruce budworm antifreeze protein interacting with ice crystals: basal plane affinity confers hyperactivity
    Natalya Pertaya
    Department of Physics and Astronomy, Ohio University, Athens, Ohio, USA
    Biophys J 95:333-41. 2008
    Antifreeze proteins (AFPs) protect certain organisms from freezing by adhering to ice crystals, thereby preventing their growth...
  77. ncbi Ice restructuring inhibition activities in antifreeze proteins with distinct differences in thermal hysteresis
    Sally O Yu
    Department of Biochemistry, Queen s University, Kingston, Ontario, Canada
    Cryobiology 61:327-34. 2010
    ..AFPs) share two related properties: the ability to depress the freezing temperature below the melting point of ice (thermal hysteresis; TH); and the ability to inhibit the restructuring of ice into larger crystals...
  78. ncbi Antifreeze proteins
    P L Davies
    Department of Biochemistry, Queens University, Kingston, Ontario, Canada
    Curr Opin Struct Biol 7:828-34. 1997
    Antifreeze proteins comprise a structurally diverse class of proteins that inhibit the growth of ice. Recently, new AFP types have been discovered; more active AFPs have been isolated; antecedents have been recognized supporting the ..
  79. ncbi A bacterial ice-binding protein from the Vostok ice core
    James A Raymond
    School of Life Sciences, University of Nevada, Las Vegas, NV 89154, USA
    Extremophiles 12:713-7. 2008
    Bacterial and yeast isolates recovered from a deep Antarctic ice core were screened for proteins with ice-binding activity, an indicator of adaptation to icy environments...
  80. ncbi Solution conformation of C-linked antifreeze glycoprotein analogues and modulation of ice recrystallization
    Roger Y Tam
    Department of Chemistry, D Iorio Hall, 10 Marie Curie, University of Ottawa, Ottawa, ON, Canada K1N 6N5
    J Am Chem Soc 131:15745-53. 2009
    ..proteins that are found in many organisms inhabiting subzero environments and ensure their survival by preventing ice growth in vivo...
  81. ncbi Bioprospecting for microbial products that affect ice crystal formation and growth
    Brent C Christner
    Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
    Appl Microbiol Biotechnol 85:481-9. 2010
    At low temperatures, some organisms produce proteins that affect ice nucleation, ice crystal structure, and/or the process of recrystallization...
  82. pmc Surviving the ice: Northern refugia and postglacial colonization
    Kevin C Rowe
    Department of Animal Biology, University of Illinois, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 101:10355-9. 2004
    ..In particular, Quaternary expansions and contractions of glacial ice sheets are thought to have played an important role in shaping the distribution of biodiversity among current ..
  83. ncbi Ecology of southern ocean pack ice
    Andrew S Brierley
    Gatty Marine Laboratory, School of Biology, University of St Andrews, Fife, KY16 8LB, UK
    Adv Mar Biol 43:171-276. 2002
    Around Antarctica the annual five-fold growth and decay of sea ice is the most prominent physical process and has a profound impact on marine life there...
  84. ncbi Extra- and intracellular ice formation in mouse oocytes
    Peter Mazur
    Fundamental and Applied Cryobiology Group, Department of Biochemistry and Cellular and Molecular Biology, The University of Tennessee, Knoxville, TN 37932 2575, USA
    Cryobiology 51:29-53. 2005
    The occurrence of intracellular ice formation (IIF) during freezing, or the lack there of, is the single most important factor determining whether or not cells survive cryopreservation...
  85. ncbi Unusual activity of the Sun during recent decades compared to the previous 11,000 years
    S K Solanki
    Max Planck Institut für Sonnensystemforschung formerly the Max Planck Institut für Aeronomie, 37191 Katlenburg Lindau, Germany
    Nature 431:1084-7. 2004
    ....
  86. ncbi The basis for hyperactivity of antifreeze proteins
    Andrew J Scotter
    Department of Biochemistry, Queen s University, Kingston, Ont, Canada K7L 3N6
    Cryobiology 53:229-39. 2006
    Antifreeze proteins (AFPs) bind to the surface of ice crystals and lower the non-equilibrium freezing temperature of the icy solution below its melting point...
  87. ncbi Analysis of intracellular ice nucleation in Xenopus oocytes by differential scanning calorimetry
    F W Kleinhans
    Fundamental and Applied Cryobiology Group, Department of Biochemistry and Cellular and Molecular Biology, The University of Tennessee, Knoxville, TN 37932, USA
    Cryobiology 52:128-38. 2006
    Intracellular ice formation (IIF) plays a central role in cell damage during cryopreservation. We are investigating the factors which trigger IIF in Xenopus oocytes, with and without aquaporin water channels...
  88. ncbi A hyperactive, Ca2+-dependent antifreeze protein in an Antarctic bacterium
    Jack A Gilbert
    Department of Biochemistry and the Protein Engineering Network of Centres of Excellence, Queen s University, Kingston, Ont, Canada K7L 3N6
    FEMS Microbiol Lett 245:67-72. 2005
    ..plants and bacteria that cannot avoid freezing use antifreeze proteins (AFPs) to lessen the destructive effects of ice recrystallization...
  89. ncbi Ice propagation in plants visualized at the tissue level by infrared differential thermal analysis (IDTA)
    Jürgen Hacker
    Institute of Botany, University of Innsbruck, 6020 Innsbruck, Austria
    Tree Physiol 27:1661-70. 2007
    We studied ice propagation in stems and leaves of various angiosperm deciduous and evergreen trees and shrubs and gymnosperms by differential thermal analysis (DTA) of thermal images captured with a digital infrared camera...
  90. ncbi A Ca2+-dependent bacterial antifreeze protein domain has a novel beta-helical ice-binding fold
    Christopher P Garnham
    Protein Function Discovery Group and the Department of Biochemistry, Queen s University, Kingston, ON, Canada K7L 3N6
    Biochem J 411:171-80. 2008
    AFPs (antifreeze proteins) are produced by many organisms that inhabit ice-laden environments. They facilitate survival at sub-zero temperatures by binding to, and inhibiting, the growth of ice crystals in solution...
  91. ncbi Ice water testing reveals hypersensitivity in adult rats that experienced neonatal bladder inflammation: implications for painful bladder syndrome/interstitial cystitis
    Alan Randich
    Department of Psychology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Urol 182:337-42. 2009
    We determined whether clinical observations of hypersensitivity to ice water testing, that is infusion of ice-cold saline into the bladder, in patients with painful bladder syndrome/interstitial cystitis have a parallel in a rat model of ..
  92. ncbi Loss of Arctic sea ice causing punctuated change in sightings of killer whales (Orcinus orca) over the past century
    Jeff W Higdon
    Department of Geography, Clayton H Riddell Faculty of Environment, Earth, and Resources, University of Manitoba, 501 University Crescent, Winnipeg, Manitoba R3T2N6, Canada
    Ecol Appl 19:1365-75. 2009
    ..Climate change models predict major reductions in sea ice with the subsequent expectation for readjustments of species' distribution and abundance...
  93. ncbi Project Ice Storm: prenatal maternal stress affects cognitive and linguistic functioning in 5 1/2-year-old children
    David P Laplante
    Douglas Hospital Research Centre, 6875 LaSalle Boulevard, Verdun, QC, Canada
    J Am Acad Child Adolesc Psychiatry 47:1063-72. 2008
    ....
  94. ncbi A diminished role for hydrogen bonds in antifreeze protein binding to ice
    H Chao
    Protein Engineering Network of Centres of Excellence, University of Alberta, Edmonton, Canada
    Biochemistry 36:14652-60. 1997
    ..It is generally assumed that HPLC-6 binds ice through a hydrogen-bonding match between the Thr and neighboring Asx residues to oxygens atoms on the {2021} plane ..
  95. ncbi Semipurification and ice recrystallization inhibition activity of ice-active substances associated with Antarctic photosynthetic organisms
    J A Raymond
    Department of Biological Sciences, University of Nevada, Las Vegas, Nevada 89154, USA
    Cryobiology 43:63-70. 2001
    b>Ice-active substances (IASs), i.e...
  96. pmc Visualization of a water-selective pore by electron crystallography in vitreous ice
    G Ren
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 98:1398-403. 2001
    ..The apex of the curved pore is close to the locations of the in-plane pseudo-2-fold symmetry axis that relates the N- and C-terminal halves and the conserved, functionally important N76 and N192 residues...
  97. ncbi Laparoscopic ice slush renal hypothermia for partial nephrectomy: the initial experience
    Inderbir S Gill
    Section of Laparascopic and Minimally Invasive Surgery, Urological Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue A 100, Cleveland, OH 44195, USA
    J Urol 170:52-6. 2003
    We describe a novel technique of laparoscopic renal hypothermia with intracorporeal ice slush during partial nephrectomy as well as clinical experience with the initial 12 patients.
  98. ncbi Dual function of the hydration layer around an antifreeze protein revealed by atomistic molecular dynamics simulations
    David R Nutt
    Computational Molecular Biophysics, IWR, Im Neuenheimer Feld 368, University of Heidelberg, 69120 Heidelberg, Germany
    J Am Chem Soc 130:13066-73. 2008
    ..the mechanism by which the antifreeze protein from the spruce budworm, Choristoneura fumiferana, binds to ice. Comparison of structural and dynamic properties of the water around the three faces of the triangular prism-shaped ..
  99. ncbi Preparation of poly(L-lactic acid) and poly(DL-lactic-co-glycolic acid) foams by use of ice microparticulates
    G Chen
    3D Tissue Engineering Group, National Institute for Advanced Interdisciplinary Research, Tsukuba, Ibaraki, Japan
    Biomaterials 22:2563-7. 2001
    ..acid) (PLGA) for tissue engineering were fabricated by a porogen-leaching technique using ice microparticulates as the porogen material. PLLA or PLGA solution in chloroform was mixed with ice microparticulates...
  100. ncbi Ice-phobic surfaces that are wet
    Howard A Stone
    Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, New Jersey 08544, USA
    ACS Nano 6:6536-40. 2012
    b>Ice formation on surfaces and structures produces damage and inefficiencies that negatively impact all manners of activities...
  101. ncbi A random control trial of contrast baths and ice baths for recovery during competition in U/20 rugby union
    Trevor R Higgins
    Center of Physical Activity Across the Lifespan, School of Exercise Science, Australian Catholic University, Sydney, Australia
    J Strength Cond Res 25:1046-51. 2011
    ..n = 26) from a premier rugby club volunteered to participate in a random control trial (RCT) using contrast baths, ice baths, and no recovery...

Research Grants76

  1. NIH Director's Pioneer Award
    Junying Yuan; Fiscal Year: 2009
    ..No Abstract provided ..
  2. SUPPORT CONTRACT FOR THE NTP INTERAGENCY CENTER/VALIDATION OF ALT METHODS
    David Allen; Fiscal Year: 2011
    ..in vitro safety testing methods, the bovine corneal opacity and permeability (BCOP) and isolated chicken eye (ICE) test methods, were accepted by U.S...
  3. SUPPORT CONTRACT FOR THE NTP INTERAGENCY CENTER/VALIDATION OF ALT METHODS
    David Allen; Fiscal Year: 2012
    ..in vitro safety testing methods, the bovine corneal opacity and permeability (BCOP) and isolated chicken eye (ICE) test methods, were accepted by U.S...
  4. Intracellular Phase Diagram Creation Using a Micro-Differential Scanning Calorime
    GARY L SOLBREKKEN; Fiscal Year: 2010
    ..with successful cryopreservation is developing cooling protocols that avoid cellular dehydration and intracellular ice formation...
  5. Targeting thermoreceptors for therapeutic hypothermia
    Sean P Marrelli; Fiscal Year: 2012
    ..Current TH protocols involve actively cooling patients by a variety of methods including cold blankets and ice baths which is slow, uncomfortable, and triggers a shiver response...
  6. Central Biochemistry Laboratory of the CKiD Consortium
    George J Schwartz; Fiscal Year: 2013
    ..optimal conditions for transporting these analytes, and provides ambient shippers for each visit and quarterly dry ice shippers for frozen specimens...
  7. Fatty Acid Synthase Inhibitors As Broad Spectrum Anti-Flaviviral Therapeutics
    Glenn Randall; Fiscal Year: 2013
    ..It inhibits FASN in cell culture and in the liver and lungs of ice. The compound is orally bioavailable and irreversibly inhibits the thioesterase domain of FASN...
  8. Effects of prenatal stress exposure on childhood physical and metabolic outcomes
    Kelsey Needham Dancause; Fiscal Year: 2013
    ..and metabolic patterns among 120 adolescents whose mothers were exposed to stress during pregnancy due to a severe ice storm in 1998;and among 262 toddlers whose mothers were pregnant during a flood in 2008...
  9. ERK Signaling in Inflammatory Bone Loss
    Francis Young In Lee; Fiscal Year: 2013
    ..An IL-1b Converting Enzyme (ICE, or Caspase-1) inhibitor showed dramatic inhibition of osteoclastogenesis...
  10. Identification and Characterization of the Mouse PPCD1 Gene
    Christopher A Bradfield; Fiscal Year: 2013
    ..notably posterior polymorphous dystrophy (PPD), and the sporadic condition, iridocorneal endothelial syndrome (ICE)...
  11. Attenuation of Ischemia-Reperfusion Injury in Limb Auto- and Allografts
    GEOFFROY COURCELLE SISK; Fiscal Year: 2013
    ..perfusion to measure histological and chemical markers of injury for comparison with control limbs preserved on ice - the current standard. Past study has shown that peptides that bind damaging IgM auto-antibodies can attenuate IRI...
  12. Proteomics and Genetics of Enamel and Dentin
    James P Simmer; Fiscal Year: 2013
    ..Their teeth are painful: they avoid hot foods, cold drinks and ice cream. One 9-year-old girl with defective enamel told us that ice cream hurts like "when you bump your elbow wrong"...
  13. MECHANISMS OF ACTIVATION OF ICE-LIKE CYSTEINE PROTEASES
    Emad S Alnemri; Fiscal Year: 2012
    ..The results of this research will help in the design and discovery of effective therapeutics to treat inflammation associated with aging, auto-inflammatory disease and chronic inflammatory diseases. ..
  14. Impact of corn grind diameter and housing environment on performance and presence
    Kenneth Anderson; Fiscal Year: 2013
    ..These tissues will be stored in sterile ice cold buffered peptone water in stomacher bags and processed at the Department of Poultry Science, for analysis for ..
  15. Assessing the Cumulative Climate-Related Health Risks in the Eastern U.S.
    Yang Liu; Fiscal Year: 2011
    Abstract Observations of increases in global average air and ocean temperatures, widespread retreat of ice sheets, and numerous other evidence signal a warming trend of our climate system...
  16. Early Detection of Pancreatic Cancer Using Ice COLD-PCR
    Grant Wu; Fiscal Year: 2012
    ..These mouse models will be used to test the application of Ice COLD-PCR to the high sensitive detection of K- RAS and Trp53 mutations in urine and blood...
  17. Factors affecting ice formation in cells and their relevance to cryopreservation
    Peter Mazur; Fiscal Year: 2011
    DESCRIPTION (provided by applicant): Intracellular ice formation (IIF) is usually lethal. The main concern of the first four years of this grant was to determine details of IIF in oocytes of mice and Xenopus and in yeast...
  18. fMRI in Binge Eating Disorder Participants Pre and Post Bariatric Surgery
    Lauren Puma; Fiscal Year: 2013
    ..g., vomiting). Binge foods usually consist of high-palatability foods (HPF), such as ice cream and pizza. Engaging in binge eating behavior may be indicative of underlying disordered neural mechanisms...
  19. Structural type 1 inositol 1,4,5-trisphosphate receptor
    Irina I Serysheva; Fiscal Year: 2010
    ..IP3R1 channel particles will be trapped in different functional states by embedding in a thin layer of vitreous ice in the presence of channel specific modulators and then directly visualized in electron cryomicroscope...
  20. Extended Storage of Tissues and Organs in Subzero Environments
    Martin L Yarmush; Fiscal Year: 2012
    ..The current gold standard for whole organ preservation is cold storage on ice for up to 72 hours, during which time the organ continuously deteriorates...
  21. Energy-based cold tolerance in genetically tractable eukaryotic systems
    Daniel H Shain; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Cold-adapted organisms ranging from psychrophilic bacteria to glacier ice worms respond to declining temperature by raising intracellular energy levels (i.e...
  22. 3D Ultrasound Guidance of Interventional Devices
    Stephen William Smith; Fiscal Year: 2012
    ..we have achieved a number of significant advances by expanding real time 3D echocardiography to intra-cardiac (3D-ICE), intra-vascular (3D-IVUS), transesophageal (3D-TEE) and thoracoscopic and laparoscopic (3D-LUS) applications by ..
  23. Development &Malleability from Childhood to Adulthood
    Nicholas S Ialongo; Fiscal Year: 2013
    ..This knowledge should determine the need for interventions aimed at improving sen/ice utilization among urban, economically disadvantaged, African-American, young adults, and the factors targeted by ..
  24. Non-Enzymatic Cryogenic Isolation of Therapeutic Cells
    MICHAEL JOHN TAYLOR; Fiscal Year: 2012
    ..distilled water or saline) via the ductal system The entire pancreas is then cooled under conditions that promote ice formation and destruction of the acinar tissue while preserving the endocrine portion by virtue of the ..
  25. Feasibility of Unfrozen Sub-zero Storage of Mammalian Tissues with Insect Anti-Fr
    Kelvin G M Brockbank; Fiscal Year: 2010
    ..The insect-derived antifreeze proteins inhibited ice nucleation at -100C permitting mammalian tissue storage at much lower levels of endogenous metabolic activity...
  26. Pulmonary Pathogen-Recognition Pathways in Melioidosis
    TIMOTHY EOIN WEST; Fiscal Year: 2013
    ..influence specific components of innate and adaptive immunity;and 3) to determine the related role of the cytosolic ICE-protease-activating factor (IPAF)/caspase-1 signaling axis in melioidosis. In performing these studies, Dr...
  27. Novel Microcapillary Technology for the Cryopreservation of Human Oocytes
    Mehmet Toner; Fiscal Year: 2010
    ..leave large mammalian cells susceptible to intracellular damage secondary to the formation of intracellular ice crystals...
  28. Institute of RNA Science and Technology (IRNAST)
    Edelgard Wulfert; Fiscal Year: 2010
    ..entire building;5) Fixed equipment includes fume hoods, autoclave, glass washer, walk-in environmental cold rooms, ice makers, water polishers, an uninterruptable power supply system, and specialty gas cylinder storage cabinets...
  29. Factors affecting ice formation in cells and their relevance to cryopreservation
    Peter Mazur; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Intracellular ice formation (IIF) is usually lethal. The main concern of the first four years of this grant was to determine details of IIF in oocytes of mice and Xenopus and in yeast...
  30. Mechanisms of Caspase-1 Mediated Schwannoma Regression
    GARY JAY BRENNER; Fiscal Year: 2013
    ..intra-tumoral injection of an adeno-associated virus (AAV) vector carrying the pro-apoptotic gene caspase-1 (ICE) under a Schwann-cell specific promoter (P0);the vector is denoted AAV-P0-ICE...
  31. Feasibility of immunotherapy by ice-free cryopreservation of engineered human all
    Kelvin G M Brockbank; Fiscal Year: 2012
    ..Our preliminary data in an in vivo allogeneic sheep model and an in vitro xenogeneic model with ice-free cryopreserved porcine tissue and human responder cells indicates that an ice- free cryopreservation method ..
  32. Optical Coherence Tomography Based Freeze Drying Microscopy
    Mircea Mujat; Fiscal Year: 2010
    ..Thin films have different ice nucleation rates, crystallization tendencies for solutes, frozen product structures and drying rates than in vial ..
  33. Rapid Induction of Therapeutic Hypothermia
    Harikrishna Tandri; Fiscal Year: 2013
    ..Current induction techniques rely on some form of active cooling, whether by ice packs, cold saline or evaporative fluids...
  34. Devices and Methods to Obviate the Risk of Ischemic Injury during Cryotherapy
    Kenneth R Diller; Fiscal Year: 2013
    ..More than 120 preliminary studies have documented that even brief exposures to circulating ice water cause a deep state of ischemia that persists for hours after the removal of cold...
  35. Minimizing the role of cryoprotectant toxicity for cryopreservation
    Utkan Demirci; Fiscal Year: 2013
    ..Vitrification (ice/crystal-free cryopreservatio) has emerged as a novel approach over traditional slow freezing methods...
  36. Optical Coherence Tomography Based Freeze Drying Microscopy
    Mircea Mujat; Fiscal Year: 2013
    ..Thin films have different ice nucleation rates, crystallization tendencies for solutes, frozen product structures and drying rates as compared to ..
  37. Cryo Upgrade of UMB EM Core Facility
    Ru Ching Hsia; Fiscal Year: 2010
    ..and Bavoil will use the plunge freeze device to prepare purified protein complexes or microorganisms in vitreous ice and subsequently perform high resolution ultrastructure analysis in the cryo TEM without the use of heavy metal ..
  38. Rat sperm cryobanking for genetic resources
    Yuksel Agca; Fiscal Year: 2010
    ..These would help reduce the injuries caused by physical, hypothermic, osmotic stress and lethal ice formation, and ultimately yield acceptable post-thaw recovery rate...
  39. Interactive Program to Manage and Prevent Back Pain
    A Blair Irvine; Fiscal Year: 2013
    ..g., heat and ice), provide information on the use of over-the-counter medication, and offer cognitive behavioral training to reduce ..
  40. Structural Studies of RyR Channel
    Irina I Serysheva; Fiscal Year: 2013
    ..This raises a number of issues with optimization of ice thickness and EM imaging to produce images with a good contrast for reliable 3D reconstruction...
  41. Hyperquenching Cryocooler for Biomolecular X-ray Crystallography
    Robert Newman; Fiscal Year: 2012
    ..This will eliminate crystalline ice formation during cooling and dramatically reduce required cryoprotectant concentrations...
  42. Dynamics of Raft Formation and Growth
    Fredric S Cohen; Fiscal Year: 2012
    ..body temperature (37[unreadable]C) are likely to be different from those of cell membranes that are kept on ice (4[unreadable]C), so many domains cannot be reliably determined at low temperature...
  43. Microencapsulation of oocytes for low-CPA (cryoprotectant) vitrification
    Xiaoming He; Fiscal Year: 2013
    ..For example, the slow-freezing (freezing: the transition of liquid water into ice crystal) approach is associated with inevitable cell injury due to ice formation and slow- freezing induced cell ..
  44. Developing a device for visualization of large-scale cryopreservation
    Yoed Rabin; Fiscal Year: 2012
    ..One of the most promising techniques for large-scale cryopreservation is known as vitrification, where ice crystallization is suppressed, and the biological material is trapped in a glassy-like state (vitreous in Latin ..
  45. Confocal imaging resources for the JHU Zebrafish Center
    Steven D Leach; Fiscal Year: 2010
    ..was established in 2008 under joint funding from the School of Medicine, the Institute for Cellular Engineering (ICE), and the Johns Hopkins McKusick-Nathans Institute of Genetic Medicine (IGM)...
  46. CRYOPRESERVATION OF DROSOPHILA MELANOGASTER EMBRYOS
    PETER STEPONKUS; Fiscal Year: 1993
    ..By using a vitrification procedure, which precludes ice formation in the system and allows for cooling at ultra-rapid rates, we have obtained 18.4 +/- 8...
  47. Computational methods for molecular tomography
    Hanspeter Winkler; Fiscal Year: 2011
    ..We can show that these methods are also useful for a variety of other biological systems, including ice-embedded samples, to characterize structural heterogeneity...
  48. PHYSIOLOGICAL MECHANISMS OF VERTEBRATE FREEZE TOLERANCE
    RICHARD LEE JR; Fiscal Year: 1991
    ..Among the vertebrates, a few species of frogs and reptiles have evolved the unique capacity to tolerate extensive ice formation within their bodies during hibernation...
  49. Oregon Institute for Comparative Effectiveness
    Cynthia D Morris; Fiscal Year: 2010
    ..The mission of the Oregon Institute for Comparative Effectiveness (Oregon ICE) is to identify, train, and mentor successful junior, mid-level, and senior faculty who have the potential to ..
  50. Microfluidic Chip for Cryopreservation of Blood Cells
    Utkan Demirci; Fiscal Year: 2012
    ..However, slow freezing leaves blood cells susceptible to intracellular damage from intracellular ice crystal formation (IIF)...
  51. Optimizing Heart and Brain Cooling during Cardiac Arrest
    Lance B Becker; Fiscal Year: 2013
    ..The overall objective of this application is to further develop the engineering to create microparticulate ice-slurry coolants for rapid cooling and determine the critical timing for cooling following prolonged arrest;we also ..
  52. The molecular and cellular basis of cold sensation
    Daniel Brenner; Fiscal Year: 2013
    ..on a borosilicate glass plate, and the glass directly underneath the rear hind paw is rapidly cooled with a dry ice pellet until the mouse withdraws its paw...
  53. A Novel, Inexpensive Cryopreservation System for Stable -80 degree C Storage of A
    Erik J Woods; Fiscal Year: 2011
    ..as -80oC, so that the cryopreserved samples can be stored and shipped using conventional "-80oC freezers" and dry ice, respectively...
  54. Cryopreservation of tissue engineered subsitutes
    Athanassios Sambanis; Fiscal Year: 2010
    ..The objective of this application is to evaluate the effect of ice-forming and ice-free cryopreservation on cell viability and construct function for a model pancreatic tissue ..
  55. Method and Device for Rodent Germplasm Vitrification
    John K Critser; Fiscal Year: 2010
    ..This is the case because (1) vitrification prevents cell damages caused by ice formation;(2) a low concentration of CPA significantly lowers CPA toxicity and osmotic damages during CPA addition ..
  56. San Diego Clinical and Translational Research Institute
    Gary S Firestein; Fiscal Year: 2013
    ..3 academic hospitals (UCSD Medical Center, VA Hospital and Rady Children's Hospital), one community health sen/ice (Palomar Pomerado Health) and 2 specialized resources~the San Diego Supercomputer Center and the California Inst, ..
  57. San Diego Clinical and Translational Research Institute
    Gary S Firestein; Fiscal Year: 2013
    ..3 academic hospitals (UCSD Medical Center, VA Hospital and Rady Children's Hospital), one community health sen/ice (Palomar Pomerado Health) and 2 specialized resources~the San Diego Supercomputer Center and the California Inst, ..
  58. Employment intervention for offenders
    Donna M Coviello; Fiscal Year: 2010
    ..This integrated counseling and employment (ICE) intervention is a manualized treatment based on interpersonal cognitive problem solving theory that has been ..
  59. Fully Integrated Single Chip CMUT Arrays for Forward Looking IVUS and ICE
    FAHRETTIN L DEGERTEKIN; Fiscal Year: 2012
    ..catheter designs have been developed, current Intravascular ultrasound (IVUS) and intra cardiac echocardiography (ICE) systems are either side-looking or do not generate the true volumetric images directly in front of the catheter...
  60. School Meals and BMI Percentile: Secondary Analyses of Non-Self-Report Data
    Suzanne Domel Baxter; Fiscal Year: 2010
    ..e., ready-to-eat cereal) vs. hot (e.g., sausage biscuit) breakfast selection, and e) ` la carte ice cream? Results will inform the design of future controlled trials and/or cohort studies to understand pathways to ..
  61. Cryo-EM 3D Reconstruction of Flexible Particles
    Hemant D Tagare; Fiscal Year: 2013
    ..is that flexibility gives rise to multiple conformations when the macromolecular particles are frozen in vitreous ice. Traditional SPR assumes that macromolecular particles are rigid, and gives good reconstructions only when all ..
  62. San Diego-Influenza Coverage Extension (SD-ICE)
    Mark H Sawyer; Fiscal Year: 2010
    ..coverage by promoting use of evidence based interventions shown to improve immunization coverage expansion (ICE)...
  63. APOPTOSIS--LENS DIFFERENTIATION AND CATARACT DEVELOPMENT
    David Li; Fiscal Year: 2000
    ..selected apoptosis-regulatory genes (Rb, p53, c-myc, c-jun, c-fos, p34cdc2, bcl-2, bcl-x, bax, bad, bag-1, mch-1, ice and ich-1)...
  64. ICE FAMILY AND NEURONAL CELL DEATH
    Junying Yuan; Fiscal Year: 1999
    DESCRIPTION: The overall objective of this research proposal is the elucidation of the roles of the ICE family in the development of the nervous system, neuronal degenerative diseases and cerebral ischemia. In the nematode C...
  65. Internal Polymeric Vessel Occluder for Temporary Vascular Occlusion of the Kidney
    PETER MADRAS; Fiscal Year: 2007
    ..Cooling the occlusion site, for example by applying ice, lowers the viscosity back to that of a liquid, dissolving the gel in blood and reestablishing blood flow...
  66. A Novel Device Design for Sperm Cryopreservation
    ERIK WOODS; Fiscal Year: 2009
    ..mechanical sensitivity, mouse sperm are vulnerable to damage associated with the morphology of the extracellular ice formed during cryopreservation...
  67. EOSINOPHIL APOPTOSIS IN ASTHMA
    Gerald Litwack; Fiscal Year: 2001
    ..The Ced- 3/ICE cysteine protease gene family are important pro-apoptotic mediators, while Bcl-2 and related proteins provide ..
  68. Freeze-Dried, Viable Allogeneic Heart Valves
    Kelvin Brockbank; Fiscal Year: 2006
    ..to the surgical point of use, the heart valves are placed either in a liquid nitrogen dry shipper or in dry ice impregnated with liquid nitrogen...
  69. ICE-FREE PRESERVATION OF OVARIES FOR LONG-TERM FECUNDITY
    Ying Song; Fiscal Year: 2004
    ..The destructive effects of extracellular ice formation caused by conventional cryopreservation by freezing are the major obstacle for cryopreservation of ..
  70. FREEZING STORAGE OF HEART AT HIGH SUBZERO TEMPERATURES
    Tingchung Wang; Fiscal Year: 1993
    ..Thus high and cytotoxic levels of synthetic cryoprotectants have to be used to avoid the lethal intracellular ice formation...
  71. MOLECULAR ICE CONTROL FOR ENGINEERED TISSUES
    Michael Taylor; Fiscal Year: 2003
    ..Cryopreservation would provide an ideal solution to this need except that ice crystallization in complex multicomponent tissues causes injury to the cells and the extracellular matrix rendering ..
  72. CASPASES IN RENAL TUBULAR EPITHELIAL CELL INJURY
    GUR KAUSHAL; Fiscal Year: 2000
    Caspases (ICE/Ced3 proteases) are a recently identified group of closely related cysteine proteases that play key roles in cell death...
  73. Virological Surveillance of influenza A in Asian lakes
    Scott Rogers; Fiscal Year: 2006
    ..We presently have summer water and winter ice samples from 2001, 2002, and 2003 and have detected influenza viruses in both types of samples...
  74. Leica EM Pact High Pressure Freezing Instrument
    M Costello; Fiscal Year: 2004
    ..The high pressure suppresses the nucleation of ice, and the coolant provides rapid fixation...
  75. STRUCTURAL STUDIES ON ANTIFREEZE PROTEINS
    FRANK SONNICHSEN; Fiscal Year: 2001
    ..e. substances which lower the freezing points of their body fluids by binding to and inhibiting the growth of seed ice crystals. Similar compounds (thermal hysteresis proteins) are currently being isolated from plants and insects...
  76. STRUCTURE-FUNCTION ANALYSIS OF ENVELOPED VIRUSES
    Wah Chiu; Fiscal Year: 1991
    ..utilizing cryo- electron microscopy have resulted in images of LAC virions, cryo- preserved in amorphous ice, containing structural information not present in the images of negative stained particles...