deuterium oxide

Summary

Summary: The isotopic compound of hydrogen of mass 2 (deuterium) with oxygen. (From Grant & Hackh's Chemical Dictionary, 5th ed) It is used to study mechanisms and rates of chemical or nuclear reactions, as well as biological processes.

Top Publications

  1. ncbi Measurement of cell proliferation by heavy water labeling
    Robert Busch
    KineMed Inc, 5980 Horton Street, Emeryville, California 94608, USA
    Nat Protoc 2:3045-57. 2007
  2. ncbi Role of heavy water in biological sciences with an emphasis on thermostabilization of vaccines
    Arnab Sen
    Senior Scientist, Division of Virology, IVRI, Mukteswar, Nainital, India
    Expert Rev Vaccines 8:1587-602. 2009
  3. ncbi Body composition in Mexican adults by air displacement plethysmography (ADP) with the BOD-POD and deuterium oxide dilution using infrared spectroscopy (IRS-DOD)
    Nayeli Macias
    Centro de Investigacion en Alimentacion y Desarrollo, Hermosillo, Sonora, Mexico
    Food Nutr Bull 23:99-102. 2002
  4. ncbi Pharmacological uses and perspectives of heavy water and deuterated compounds
    D J Kushner
    Department of Botany, University of Toronto, ON, Canada
    Can J Physiol Pharmacol 77:79-88. 1999
  5. ncbi The medical-irradiation characteristics for neutron capture therapy at the Heavy Water Neutron Irradiation Facility of Kyoto University Research Reactor
    Yoshinori Sakurai
    Kyoto University Research Reactor Institute, Sennan gun, Osaka, Japan
    Med Phys 29:2328-37. 2002
  6. pmc Deuterium-resistant algal cell line for D labeling of heterotrophs expresses enhanced level of Hsp60 in D2O medium
    Keiko Unno
    Department of Medical Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52 1 Yada, Shizuoka 422 8526, Japan
    Appl Environ Microbiol 71:2256-9. 2005
  7. ncbi Effects of heavy water (D2O) on human pancreatic tumor cells
    Johannes Hartmann
    Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Vienna, General Hospital of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    Anticancer Res 25:3407-11. 2005
  8. ncbi Body composition prediction equations based on deuterium oxide dilution method in Mexican children: a national study
    E Ramirez
    Laboratorio de Composición Corporal y Gasto Energético, Centro de Investigación en Nutrición y Salud Pública, Facultad de Salud Pública y Nutrición, Universidad Autonoma de Nuevo Leon, Nuevo Leon, Mexico
    Eur J Clin Nutr 66:1099-103. 2012
  9. pmc Effect of heavy water on protein flexibility
    Patrizia Cioni
    Istituto di Biofisica, Consiglio Nazionale delle Ricerche, Area della Ricerca di Pisa, Pisa, Italy
    Biophys J 82:3246-53. 2002
  10. ncbi Assessment of body fat in the pony: part II. Validation of the deuterium oxide dilution technique for the measurement of body fat
    A H A Dugdale
    University of Liverpool, School of Veterinary Science, Leahurst, Neston
    Equine Vet J 43:562-70. 2011

Detail Information

Publications237 found, 100 shown here

  1. ncbi Measurement of cell proliferation by heavy water labeling
    Robert Busch
    KineMed Inc, 5980 Horton Street, Emeryville, California 94608, USA
    Nat Protoc 2:3045-57. 2007
    ..The protocols enable definitive in vivo studies of the fraction or absolute number of newly divided cells and their subsequent survival kinetics in animals and humans...
  2. ncbi Role of heavy water in biological sciences with an emphasis on thermostabilization of vaccines
    Arnab Sen
    Senior Scientist, Division of Virology, IVRI, Mukteswar, Nainital, India
    Expert Rev Vaccines 8:1587-602. 2009
    ..b>Deuterium oxide (heavy water; D(2)O) has previously been reported to have a protective effect on biomolecules (proteins and ..
  3. ncbi Body composition in Mexican adults by air displacement plethysmography (ADP) with the BOD-POD and deuterium oxide dilution using infrared spectroscopy (IRS-DOD)
    Nayeli Macias
    Centro de Investigacion en Alimentacion y Desarrollo, Hermosillo, Sonora, Mexico
    Food Nutr Bull 23:99-102. 2002
    ..for body density by air displacement plethysmography (ADP) with the BOD-POD, and for total body water by deuterium oxide dilution and infrared spectroscopy (IRS-DOD). Subjects were given a 30 g dose of deuterium oxide...
  4. ncbi Pharmacological uses and perspectives of heavy water and deuterated compounds
    D J Kushner
    Department of Botany, University of Toronto, ON, Canada
    Can J Physiol Pharmacol 77:79-88. 1999
    ..Deuterated insecticides might well be more effective against resistant insects, but this potentially valuable possibility has not yet been studied...
  5. ncbi The medical-irradiation characteristics for neutron capture therapy at the Heavy Water Neutron Irradiation Facility of Kyoto University Research Reactor
    Yoshinori Sakurai
    Kyoto University Research Reactor Institute, Sennan gun, Osaka, Japan
    Med Phys 29:2328-37. 2002
    ..Besides, in the viewpoint of the radiation shielding for patient, it was confirmed that the whole-body exposure is effectively reduced by the new clinical collimators, compared with the old one...
  6. pmc Deuterium-resistant algal cell line for D labeling of heterotrophs expresses enhanced level of Hsp60 in D2O medium
    Keiko Unno
    Department of Medical Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52 1 Yada, Shizuoka 422 8526, Japan
    Appl Environ Microbiol 71:2256-9. 2005
    ..Unno, T. Kishido, M. Morioka, S. Okada, and N. Oku, Biol. Pharm. Bull. 26:799-802, 2003). This suggests that the overexpression of heat shock proteins is required to compensate for the deuterium isotope effect...
  7. ncbi Effects of heavy water (D2O) on human pancreatic tumor cells
    Johannes Hartmann
    Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Vienna, General Hospital of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    Anticancer Res 25:3407-11. 2005
    ..Since heavy water (deuterium oxide, D2O) was shown to be active in various cancer cell lines in vitro and in vivo, we now investigated its ..
  8. ncbi Body composition prediction equations based on deuterium oxide dilution method in Mexican children: a national study
    E Ramirez
    Laboratorio de Composición Corporal y Gasto Energético, Centro de Investigación en Nutrición y Salud Pública, Facultad de Salud Pública y Nutrición, Universidad Autonoma de Nuevo Leon, Nuevo Leon, Mexico
    Eur J Clin Nutr 66:1099-103. 2012
    ..study was to facilitate assessments of body composition in Mexican school children of different geographical regions and ethnicity by developing equations for bioelectrical impedance and anthropometry based on deuterium oxide dilution.
  9. pmc Effect of heavy water on protein flexibility
    Patrizia Cioni
    Istituto di Biofisica, Consiglio Nazionale delle Ricerche, Area della Ricerca di Pisa, Pisa, Italy
    Biophys J 82:3246-53. 2002
    ..Structure tightening by heavy water is generally amplified at higher temperatures, supporting a mostly hydrophobic nature of the underlying interaction, and under conditions that destabilize the globular fold...
  10. ncbi Assessment of body fat in the pony: part II. Validation of the deuterium oxide dilution technique for the measurement of body fat
    A H A Dugdale
    University of Liverpool, School of Veterinary Science, Leahurst, Neston
    Equine Vet J 43:562-70. 2011
    ..An objective, minimally-invasive method to accurately quantify body fat in living animals is required to aid nutritional management and define welfare/performance limits...
  11. ncbi Effects of deuterium oxide on Streptococcus mutans and Pseudomonas aeruginosa
    Kaname Hirai
    Department of Microbiology, Matsumoto Dental University, Nagano, Japan
    Bull Tokyo Dent Coll 51:175-83. 2010
    ..It is well known that deuterium oxide (D₂O) causes toxic effects on a number of biological systems...
  12. ncbi Deuterium oxide stabilizes conformation of tubulin:a biophysical and biochemical study
    Amlan Das
    Dr B C Guha Centre for Genetic Engineering and Biotechnology, University of Calcutta, Kolkata, India
    BMB Rep 41:62-7. 2008
    The present study was aimed to elucidate the mechanism of stabilization of tubulin by deuterium oxide (D(2)O)...
  13. pmc Water and deuterium oxide permeability through aquaporin 1: MD predictions and experimental verification
    Artem B Mamonov
    Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA
    J Gen Physiol 130:111-6. 2007
    ..The combined computational and experimental results suggest that deuterium oxide permeability through AQP1 is similar to that of water...
  14. ncbi Effect of deuterium oxide on the thermodynamic quantities associated with phase transitions of phosphatidylcholine bilayer membranes
    Hitoshi Matsuki
    Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima, Japan
    Biochim Biophys Acta 1712:92-100. 2005
    ..DPPC), distearoylphosphatidylcholine (DSPC) and dihexadecylphosphatidylcholine (DHPC), in deuterium oxide (D(2)O) and hydrogen oxide (H(2)O) were observed by differential scanning calorimetry (DSC) under ambient ..
  15. ncbi Plasma deuterium oxide accumulation following ingestion of different carbohydrate beverages
    Kevin Currell
    School of Sport and Exercise Sciences, University of Birmingham, Edgbaston, Birmingham, B152TT, UK
    Appl Physiol Nutr Metab 33:1067-72. 2008
    ..Plasma and saliva samples were analyzed for deuterium enrichment by isotope ratio mass spectrometry. Deuterium oxide enrichments were compared using a 2-way repeated measures analysis of variance...
  16. ncbi Effect of physiological hyperinsulinemia on gluconeogenesis in nondiabetic subjects and in type 2 diabetic patients
    A Gastaldelli
    Metabolism Unit of the C.N.R. Institute of Clinical Physiology and the Department of Internal Medicine, University of Pisa School of Medicine, Pisa, Italy
    Diabetes 50:1807-12. 2001
    ..In type 2 diabetes, the excess GNG of the fasting state is carried over to the insulinized state, thereby contributing to glucose overproduction under both conditions...
  17. ncbi Determination of protein replacement rates by deuterated water: validation of underlying assumptions
    Emmanuelle Belloto
    1Institut National de la Santé et de la Recherche Médicale U499, Faculté Réne Theodore Hyacinthe, Laennec, France
    Am J Physiol Endocrinol Metab 292:E1340-7. 2007
    ..All of these results support (2)H(2)O as a safe, reliable, useful, and convenient tracer for studies of protein synthesis, including proteins with slow turnover rate...
  18. ncbi Implications of the variability in time to isotopic equilibrium in the deuterium dilution technique
    R C Colley
    School of Human Movement Studies, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia
    Eur J Clin Nutr 61:1250-5. 2007
    ..To investigate the variability in isotopic equilibrium time under field conditions, and the impact of this variability on estimates of total body water (TBW) and body composition...
  19. ncbi Measurement of protein turnover rates by heavy water labeling of nonessential amino acids
    Robert Busch
    KineMed, Inc, Emeryville, CA 94608, USA
    Biochim Biophys Acta 1760:730-44. 2006
    ..In conclusion, 2H2O labeling permits sensitive, quantitative, operationally simple measurements of protein turnover in vivo by the rise-to-plateau approach, especially for proteins with slow constitutive turnover...
  20. ncbi Rapid measurement of deuterium content of breath following oral ingestion to determine body water
    S Davies
    Centre for Science and Technology in Medicine, School of Postgraduate Medicine, Keele University, UK
    Physiol Meas 22:651-9. 2001
    ..We conclude that FA-MS is a powerful new method that, when combined with oral D2O loading, enables measurement of the dispersal kinetics of HDO and the calculation of total body water within 2 hours...
  21. ncbi Total energy expenditure in the Yakut (Sakha) of Siberia as measured by the doubly labeled water method
    J Josh Snodgrass
    Department of Anthropology, University of Oregon, Eugene, OR 97403, USA
    Am J Clin Nutr 84:798-806. 2006
    ..Although reduced physical activity has been implicated, few studies have accurately quantified energy expenditure in subsistence populations...
  22. ncbi Quantifying tracer levels of (2)H(2)O enrichment from microliter amounts of plasma and urine by (2)H NMR
    J G Jones
    Mary Nell and Ralph B Rogers Magnetic Resonance Center, Department of Radiology, UT Southwestern Medical Center, Dallas, Texas 75235 9085, USA
    Magn Reson Med 45:156-8. 2001
    ..The measurement is insensitive to the presence of plasma protein, allowing direct analysis of (2)H(2)O enrichment from native plasma samples. Magn Reson Med 45:156-158, 2001...
  23. ncbi Measuring the turnover rates of Arabidopsis proteins using deuterium oxide: an auxin signaling case study
    Xiao Yuan Yang
    Department of Plant Biology, University of Minnesota, St Paul, MN 55108, USA
    Plant J 63:680-95. 2010
    ..These considerations suggest a prescription for specific experimental designs that minimize interference resulting from the induction of treatment-specific gene expression in the results obtained...
  24. ncbi Age-related degeneration of lumbar intervertebral discs in rabbits revealed by deuterium oxide-assisted MRI
    V Y L Leung
    Department of Orthopaedics and Traumatology, The University of Hong Kong, Hong Kong SAR, China
    Osteoarthritis Cartilage 16:1312-8. 2008
    ..This study addresses whether rabbits undergo age-related disc degeneration, assessed using deuterium oxide-assisted magnetic resonance imaging (MRI) of the lumbar IVDs.
  25. ncbi Body composition by hydrometry (deuterium oxide dilution) and bioelectrical impedance in subjects aged >60 y from rural regions of Cuba, Chile and Mexico
    M E Valencia
    División de Nutrición, Centro de Investigacion en Alimentacion y Desarrollo, A C, Hermosillo, Sonora, Mexico
    Int J Obes Relat Metab Disord 27:848-55. 2003
    ..In Latin American and Caribbean countries such as Chile, Mexico and Cuba, the population over 60 y has increased steadily. In this age group, there is scarce information about body composition, particularly for those living in rural areas...
  26. ncbi Using 2H2O to study the influence of feeding on protein synthesis: effect of isotope equilibration in vivo vs. in cell culture
    Danielle A Dufner
    Dept of Nutrition, D 201, CWRU School of Medicine, Cleveland, OH 44106, USA
    Am J Physiol Endocrinol Metab 288:E1277-83. 2005
    ..In particular, since there can be slow equilibration of 2H between water and alanine in the cell culture medium, special consideration must be made to avoid underestimating the rate of protein synthesis in vitro...
  27. ncbi Validity of deuterium oxide dilution for the measurement of body fat among Singaporeans
    Mabel Deurenberg-Yap
    National University of Singapore
    Food Nutr Bull 23:34-7. 2002
    ..fat percent (BF%) was measured in 108 adult Chinese, 76 Malays, and 107 Indians in Singapore by densitometry, deuterium oxide dilution (hydrometry), dual energy x-ray absorptiometry (DXA) and a chemical four-compartment model (BF%4c)...
  28. ncbi Quantifying rates of protein synthesis in humans by use of 2H2O: application to patients with end-stage renal disease
    Stephen F Previs
    Department of Medicine, Case Western Reserve University School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106 4951, USA
    Am J Physiol Endocrinol Metab 286:E665-72. 2004
    ..Estimates are in subjects with renal disease, but the method should be applicable to estimates of protein synthetic rates in normal subjects and in other pathological states...
  29. ncbi Food-entrained circadian rhythms in rats are insensitive to deuterium oxide
    R E Mistlberger
    Department of Psychology, Simon Fraser University, 8888 University Drive, Burnaby, BC V5A 1S6, Canada
    Brain Res 919:283-91. 2001
    ..D(2)O may have utility as a screening test to identify putative food-entrainable pacemakers from among those central and peripheral tissues that can express circadian oscillations of clock genes independent of the SCN...
  30. ncbi Measurement of milk intake in suckling llamas (Lama glama) using deuterium oxide dilution
    A Riek
    Institute of Animal Breeding and Genetics, University of Goettingen, Albrecht Thaer Weg 3, D 37075 Goettingen, Germany
    J Dairy Sci 90:867-75. 2007
    ..Milk intake in 11 suckling llamas was estimated from water kinetics using deuterium oxide (D2O) at d 17, 66, and 128 postpartum. Daily milk intakes averaged 2.6, 2.3, and 2...
  31. ncbi Measurement of liver collagen synthesis by heavy water labeling: effects of profibrotic toxicants and antifibrotic interventions
    James L Gardner
    Dept of Nutritional Sciences and Toxicology, Univ of California, Berkeley, CA 94720, USA
    Am J Physiol Gastrointest Liver Physiol 292:G1695-705. 2007
    ....
  32. pmc Deuterium isotope effects on permeation and gating of proton channels in rat alveolar epithelium
    T E Decoursey
    Department of Molecular Biophysics and Physiology, Rush Presbyterian St Luke s Medical Center, Chicago, Illinois 60612, USA
    J Gen Physiol 109:415-34. 1997
    ..The effects of substituting deuterium oxide, D2O, for water, H2O, on both the conductance and the pH dependence of gating were explored...
  33. pmc Isotopologue distributions of peptide product ions by tandem mass spectrometry: quantitation of low levels of deuterium incorporation
    Benlian Wang
    Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA
    Anal Biochem 367:40-8. 2007
    ..The ability to determine the isotopic composition of sequential product ions permits the isotopic composition of individual amino acid residues in the precursor ion to be determined...
  34. ncbi Heavy water labeling of keratin as a non-invasive biomarker of skin turnover in vivo in rodents and humans
    Glen Lindwall
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California 94720, USA
    J Invest Dermatol 126:841-8. 2006
    ..In summary, 2H2O labeling of skin keratin represents a non-invasive approach for assessing skin turnover dynamics in pre-clinical models and in human subjects...
  35. ncbi Accumulation of 2H2O in plasma and eccrine sweat during exercise-heat stress
    Lawrence E Armstrong
    Human Performance Laboratory, University of Connecticut, Storrs, CT 06269 1110, USA
    Eur J Appl Physiol 108:477-82. 2010
    ..b>Deuterium oxide ((2)H(2)O) accumulation in plasma and eccrine sweat was measured at two sites (back and forehead)...
  36. pmc Measurement of protein synthesis using heavy water labeling and peptide mass spectrometry: Discrimination between major histocompatibility complex allotypes
    Alessandra de Riva
    Department of Medicine, University of Cambridge, Cambridge CB2 0QQ, UK
    Anal Biochem 403:1-12. 2010
    ..It simplifies measurements of protein synthesis, enabling novel applications in physiology, in genotype/phenotype interactions, and potentially in kinetic proteomics...
  37. ncbi Determination of protein synthesis in vivo using labeling from deuterated water and analysis of MALDI-TOF spectrum
    Gary Guishan Xiao
    Department of Pediatrics, Division of Endocrinology, Los Angeles Biomedical Research Institute at Harbor UCLA, 1124 W Carson St, Torrance, CA 90502, USA
    J Appl Physiol (1985) 104:828-36. 2008
    ..The combined use of postsource decay sequence identification and mass isotopomer distribution analysis makes the use of in vivo labeling with deuterated water a precise method to determine specific protein synthesis...
  38. ncbi Measurement of gluconeogenesis by deuterated water: the effect of equilibration time and fasting period
    Gideon Allick
    Dept of Endocrinology and Metabolism, Academic Medical Center, PO Box 22700, 1100 DD Amsterdam, The Netherlands
    Am J Physiol Endocrinol Metab 290:E1212-7. 2006
    ..After 5- and 15-h 2H2O incubation, GNG can be reliably measured with C5/2H2O. Gluconeogenetic labeling of glycogen did not affect the results after 3, 5, or 15 h of 2H2O incubation...
  39. ncbi Effects of deuterium oxide and temperature on heart rate in Drosophila melanogaster
    L A White
    Department of Zoology, University of Maine, Orono 04469
    J Comp Physiol B 162:278-83. 1992
    ..Heartbeat in this insect is an oscillation that is not temperature compensated. Deuterium oxide lengthens the period of a number of high and low frequency oscillators and clocks in a variety of organisms...
  40. ncbi Do ethanol and deuterium oxide distribute into the same water space in healthy volunteers?
    A Norberg
    Department of Anesthesiology and Intensive Care, Karolinska Institute at Huddinge University Hospital, Huddinge, Sweden
    Alcohol Clin Exp Res 25:1423-30. 2001
    ..Ethanol concentration-time profiles were established for breath, venous blood, and urine. The values of Vss obtained for ethanol were compared with TBW determined by deuterium oxide dilution.
  41. ncbi High-resolution field-cycling NMR studies of a DNA octamer as a probe of phosphodiester dynamics and comparison with computer simulation
    Mary F Roberts
    Department of Chemistry, Boston College, Chestnut Hill, Massachusetts 02467, USA
    Biochemistry 43:3637-50. 2004
    ..High-resolution field cycling opens up the possibility of monitoring residue-specific dipolar interactions and dynamics for the phosphorus nuclei of diverse oligonucleotides...
  42. pmc Uncovering the hidden ground state of green fluorescent protein
    John T M Kennis
    Department of Biophysics, Faculty of Sciences, Vrije Universiteit Amsterdam, De Boelelaan 1081, Amsterdam 1018HV, The Netherlands
    Proc Natl Acad Sci U S A 101:17988-93. 2004
    ..We have resolved the true reaction rate of a proton transfer in the molecular ground state of GFP, and our results may thus aid in the development of a generic understanding of proton transfer in biology...
  43. ncbi Spectroscopic studies of methylglyoxal in water and dimethylsulfoxide
    Ina Nemet
    Ruder Boskovic Institute, P O B 180, 10 002 Zagreb, Croatia
    Bioorg Chem 32:560-70. 2004
    ..1H and 13C NMR spectroscopy were used to determine the structures of the different monomeric and oligomeric structures of methylglyoxal...
  44. pmc The influence of solvent composition on global dynamics of human butyrylcholinesterase powders: a neutron-scattering study
    F Gabel
    Laboratoire de Biophysique Moleculaire, Institut de Biologie Structurale, Grenoble, France
    Biophys J 86:3152-65. 2004
    ..We draw the conclusion that the reduction of intramolecular protein mean-square displacements on an Angstrom-nanosecond scale by the solvent depends not only on the presence of salt ions but also on their type...
  45. ncbi Protein instability during HIC: describing the effects of mobile phase conditions on instability and chromatographic retention
    Yunzhi Xiao
    Department of Chemical Engineering, University of Virginia, 102 Engineers Way, Charlottesville, 22904 4741, USA
    Biotechnol Bioeng 93:1177-89. 2006
    ..The model successfully describes the effects of added calcium and ammonium sulfate. The results demonstrate the importance of considering the effects on stability of mobile phase modifiers when applying HIC to marginally stable..
  46. ncbi Proton channel hydration and dynamics of a bacteriorhodopsin triple mutant with an M-state-like conformation
    U Lehnert
    Max Planck Institut fur Biochemie, 82152 Martinsried, Germany
    Eur Biophys J 34:344-52. 2005
    ....
  47. ncbi Proton and deuteron position preferences in water clusters: an ab initio study
    David J Anick
    Harvard Medical School, Harvard University, McLean Hospital, Belmont, Massachusetts 02478, USA
    J Chem Phys 123:244309. 2005
    ..82 for the disproportionation equilibrium constant of mixed ice at 150 K, increased occupation by H at surface positions of mixed ice, and a larger average coordination number for liquid D2O than for liquid H2O...
  48. ncbi Water-soluble molecular capsules: self-assembly and binding properties
    Francesca Corbellini
    Laboratory of Supramolecular Chemistry and Technology, MESA Institute for Nanotechnology, University of Twente, P O Box 217, 7500 AE Enschede, The Netherlands
    Chemistry 11:298-307. 2004
    ..Experimental guest encapsulation studies indicate that capsule 1 a2 is an effective host for both charged (N-methylquinuclidinium cation) and neutral molecules (6-amino-2-methylquinoline) in water...
  49. ncbi Water-mediated hydrogen-bonded network on the cytoplasmic side of the Schiff base of the L photointermediate of bacteriorhodopsin
    Akio Maeda
    Department of Biochemistry, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Biochemistry 42:14122-9. 2003
    ..This structure, extending from the Schiff base to the internal proton donor Asp96, stabilizes L and affects the L-to-M transition...
  50. pmc What really prevents proton transport through aquaporin? Charge self-energy versus proton wire proposals
    Anton Burykin
    Department of Chemistry, University of Southern California, Los Angeles, California, USA
    Biophys J 85:3696-706. 2003
    ..However, in cases of biological channels, the actual PT mechanism is much less important than the energetics of transferring the proton charge from water to different regions in the channels...
  51. pmc High frequency dynamics in hemoglobin measured by magnetic relaxation dispersion
    Ken Victor
    Chemistry Department, University of Virginia, Charlottesville, Virginia, USA
    Biophys J 88:443-54. 2005
    ....
  52. ncbi Effect of nucleoside reverse transcriptase inhibitors on mitochondrial DNA synthesis in rats and humans
    Michelle L Collins
    Department of Nutritional Sciences and Toxicology, University of California at Berkeley, CA 94720 3104, USA
    J Acquir Immune Defic Syndr 37:1132-9. 2004
    ....
  53. ncbi OH-stretch vibrational relaxation of HOD in liquid to supercritical D2O
    Dirk Schwarzer
    Max Planck Institut fur biophysikalische Chemie, Am Fassberg 11, D 37077 Gottingen, Germany
    J Phys Chem A 110:2858-67. 2006
    ....
  54. ncbi Structural phase transition of monoclinic crystals of hen egg-white lysozyme
    Kazuaki Harata
    Biological Information Research Center, National Institute of Advanced Industrial Science and Technology AIST, Central 6, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Acta Crystallogr D Biol Crystallogr 62:375-82. 2006
    ....
  55. ncbi Salt-induced micellization of a triblock copolymer in aqueous solution: a 1H nuclear magnetic resonance spectroscopy study
    Jun He Ma
    Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Graduate School of Chinese Academy of Sciences, Beijing 100080, PR China
    Langmuir 23:3075-83. 2007
    ..0 mol l-1 for KCl in 2.5% aqueous pluronic F88 solution at 25 degrees C, and the transition region in which both free and associated copolymer molecules coexist is defined to range from 1 to 2 mol L-1...
  56. ncbi Micellization in aqueous solution of an ethylene oxide-propylene oxide triblock copolymer, investigated with 1H NMR spectroscopy, pulsed-field gradient NMR, and NMR relaxation
    Junhe Ma
    Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Graduate School of Chinese Academy of Sciences, Beijing 100080, China
    J Colloid Interface Sci 312:390-6. 2007
    ..Thermodynamics analysis according to a closed association model shows that the micellization process is entropy-driven and has an endothermic micellization enthalpy...
  57. ncbi A mechanistic and kinetic study of the beta-lactone hydrolysis of Salinosporamide A (NPI-0052), a novel proteasome inhibitor
    Nunzio Denora
    Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence, Kansas 66047, USA
    J Pharm Sci 96:2037-47. 2007
    ..15 M adjusted with NaCl) at various pH values in the range of 1 to 9. Degradation kinetics in water and in deuterium oxide were compared as a mechanistic probe. The studies were performed at pH (pD) 4.5 at 25 degrees C...
  58. ncbi Polymer mobilization and drug release during tablet swelling. A 1H NMR and NMR microimaging study
    Carina Dahlberg
    YKI, Institute for Surface Chemistry, Stockholm, Sweden
    J Control Release 122:199-205. 2007
    ....
  59. ncbi Solvent and solvent isotope effects on the vibrational cooling dynamics of a DNA base derivative
    Chris T Middleton
    Department of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA
    J Phys Chem A 111:10460-7. 2007
    ....
  60. ncbi Comparative study of the interaction of pyridine with polycrystalline Ag and amorphous solid water
    S Bahr
    Institut fur Physik und Physikalische Technologien, Technische Universitat Clausthal, Leibnizstrasse 4, D 38678 Clausthal Zellerfeld, Germany
    J Chem Phys 127:174514. 2007
    ..The exposure of a pyridine film at 124 K to D2O molecules does not lead to on top adsorption. Instead, D2O becomes initially embedded into the pyridine film, and RAIRS indicates solvation of the pyridine species...
  61. ncbi Investigations on the structure of dimethyl sulfoxide and acetone in aqueous solution
    Sylvia E McLain
    Spallation Neutron Source and Center for Molecular Biophysics, Oak Ridge National Laboratory, PO Box 2008, Oak Ridge, Tennessee 37831 6475, USA
    J Chem Phys 127:174515. 2007
    ..This result may have implications for the highly nonideal behavior observed in the thermodynamic functions for 1:2 DMSO-water solutions...
  62. ncbi Detection of specific solvent rearrangement regions of an enzyme: NMR and ITC studies with aminoglycoside phosphotransferase(3')-IIIa
    Can Ozen
    Graduate School of Genome Science and Technology, University of Tennessee and Oak Ridge National Laboratories, Knoxville, Tennessee 37996 0840, USA
    Biochemistry 47:40-9. 2008
    ....
  63. ncbi Computational studies of liquid water and diluted water in carbon tetrachloride
    Tsun Mei Chang
    Department of Chemistry, University of Wisconsin Parkside, 900 Wood Road, Box 2000, Kenosha, Wisconsin 53141, USA
    J Phys Chem A 112:1694-700. 2008
    ..In addition, we found the rotational correlation times to be substantially faster in liquid CCl4 than in liquid water...
  64. ncbi Potential energy surface of HDO up to 25,000 cm-1
    S N Yurchenko
    Institut fur Physikalische Chemie und Elektrochemie, Technische Universitat Dresden, D 01062 Dresden, Germany
    J Chem Phys 128:044312. 2008
    ..The new HDO_07 PES reproduces the experimental data, including high J levels not included in the fit, with a root mean square error of 0.035 cm(-1). Predictions for rotation-vibration term values up to J=12 are made...
  65. ncbi Heterogeneity of water at the phospholipid membrane interface
    Victor V Volkov
    European Laboratory for Nonlinear Spectroscopy LENS, University of Florence, Via Nello Carrara 1, 50019 Sesto Fiorentino, Italy
    J Phys Chem B 111:1377-83. 2007
    ..This specific character appears to have a direct effect on the structural stability and thermal properties of the membrane...
  66. ncbi Vibrational analysis of amino acids and short peptides in hydrated media. I. L-glycine and L-leucine
    Najoua Derbel
    Laboratoire de Spectroscopie Atomique Moléculaire et Applications LASMA, Departement de Physique, Faculte des Sciences de Tunis, Campus Universitaire, 2092 EL MANAR II, Tunisia
    J Phys Chem B 111:1470-7. 2007
    ....
  67. ncbi Solution structure of a Hck SH3 domain ligand complex reveals novel interaction modes
    Holger Schmidt
    Forschungszentrum Julich GmbH, INB, Biomolecular NMR, 52425 Julich, Germany
    J Mol Biol 365:1517-32. 2007
    ..Consequences for computational prediction tools adressing SH3-ligand interactions as well as the biological relevance of our findings are discussed...
  68. ncbi Validation of the drop coating deposition Raman method for protein analysis
    Corasi Ortiz
    Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA
    Anal Biochem 353:157-66. 2006
    ....
  69. ncbi Purification and characterization of Thermotoga maritima homoserine transsuccinylase indicates it is a transacetylase
    Maryam Goudarzi
    Department of Chemistry and Biochemistry, George Mason University, 10900 University Boulevard, Manassas, VA 20110, USA
    Extremophiles 10:469-78. 2006
    ..Taken together, these data suggest that the T. maritima enzyme functions biochemically as a transacetylase, despite having the sequence of a transsuccinylase...
  70. ncbi Determination of the hydration number of gadolinium(III) complexes by high-field pulsed 17O ENDOR spectroscopy
    Arnold M Raitsimring
    Department of Chemistry, University of Arizona, Tucson, AZ 85721, USA
    Chemphyschem 7:1590-7. 2006
    ..Under the experimental conditions used for the hydration number determination (30 mol % methanol), fewer than 15 % of the coordination sites were found to be occupied by methanol...
  71. ncbi Two competitive routes in the lactim-lactam phototautomerization of a hydroxypyridine derivative cation in water: dissociative mechanism versus water-assisted proton transfer
    J Carlos Penedo
    Departamento de Quimica Fisica, Facultade de Quimica, Universidade de Santiago de Compostela, E 15782 Santiago de Compostela, Spain
    J Phys Chem A 109:10189-98. 2005
    ....
  72. ncbi Dynamics of amide-I modes of the alanine dipeptide in D2O
    Yung Sam Kim
    Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6323, USA
    J Phys Chem B 109:6884-91. 2005
    ..The intermediate time scale is attributed to fluctuations in the stretching and bending of hydrogen bonds between the carbonyl and water...
  73. ncbi Hydrogen-bonding interaction of the protonated schiff base with halides in a chloride-pumping bacteriorhodopsin mutant
    Mikihiro Shibata
    Department of Materials Science and Engineering, Nagoya Institute of Technology, Showa Ku, Nagoya 466 8555, Japan
    Biochemistry 45:10633-40. 2006
    ..Hydrogen-bonding conditions in the Schiff base region of D85S are discussed on the basis of the spectral comparison with those of wild-type BR and HR...
  74. ncbi Orientational dynamics of isotopically diluted H2O and D2O
    Y L A Rezus
    FOM Institute for Atomic and Molecular Physics, Kruislaan 407, 1098 SJ Amsterdam, The Netherlands
    J Chem Phys 125:144512. 2006
    ..We construct a model that includes this effect and is able to reproduce the experimental data. Using this model we show how the distorted anisotropy can be corrected...
  75. pmc Intracellular proton regulation of ClC-0
    Giovanni Zifarelli
    Istituto di Biofisica, Consiglio Nazionale delle Ricerche, I 16149 Genova, Italy
    J Gen Physiol 132:185-98. 2008
    ..The gating model is in natural agreement with the finding that only the closing rate constant, but not the opening rate constant, depends on pH(int) and [Cl(-)](int)...
  76. pmc The guanine cation radical: investigation of deprotonation states by ESR and DFT
    Amitava Adhikary
    Department of Chemistry, Oakland University, Rochester, Michigan 48309, USA
    J Phys Chem B 110:24171-80. 2006
    ....
  77. ncbi Probing the behavior of confined water by proton-transfer reactions
    G Angulo
    Departamento de Quimica Fisica, Sección de Químicas, Facultad de Ciencias del Medio Ambiente, Universidad de Castilla La Mancha, Avenida Carlos III, S N, 45071 Toledo, Spain
    J Phys Chem B 110:24231-7. 2006
    ..The lower fluidity of confined water within the reverse micelle with respect to normal bulk water alters the related H-bond network dynamics and therefore is responsible for the slower proton-transfer processes...
  78. ncbi Origin of the nonexponential dynamics of excited-state proton transfer in wt-green fluorescent protein
    R Gepshtein
    Raymond and Beverly Sackler Faculty of Exact Sciences, School of Chemistry, Tel Aviv University, Tel Aviv 69978, Israel
    J Phys Chem B 112:7203-10. 2008
    ..We attribute the inhomogeneous kinetics to the distance dependence of the excited-state proton-transfer rate between the proton donor (the hydroxyl group of the chromophore) and the oxygen of a nearby water molecule...
  79. ncbi Interactions of cholesterol with cyclodextrins in aqueous solution
    Juziro Nishijo
    Kobe Pharmaceutical University, Higashinada ku, Kobe, Japan
    Chem Pharm Bull (Tokyo) 51:1253-7. 2003
    ..Also, based on the measurements of proton nuclear magnetic resonance spectra and studies with Corey-Pauling-Koltun atomic models, the probable structutures of the 1 : 2 complex were estimated...
  80. ncbi Electron transfer in deuterated reaction centers of Rhodobacter sphaeroides at 90 K according to femtosecond spectroscopy data
    A G Yakovlev
    Department of Photobiophysics, Belozersky Institute of Physico Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia
    Biochemistry (Mosc) 68:603-10. 2003
    ....
  81. ncbi [Protective effect of D20 in bacteria (E. coli)]
    C Papadimitriou
    , , Bundesrepublik Deutschland
    Z Naturforsch C 44:1053-7. 1989
    ..On the contrary E. coli grown in D2O-containing media - therefore being fully deuterated - were more sensitive than normally grown bacteria...
  82. ncbi Insight into the catalysis of hydrolysis of four newly synthesized substrates by papain: a proton inventory study
    L G Theodorou
    University of Ioannina, Department of Chemistry, Laboratory of Biochemistry, 451-10 Ioannina, Greece
    Biochemistry 40:3996-4004. 2001
    ..Furthermore, papain acts as a one-proton catalyst during acylation or deacylation, both of which proceed through similar concerted reaction pathways, where a nucleophilic attack is accompanied by the movement of one proton...
  83. ncbi Kinetic and mechanistic studies of prolyl oligopeptidase from the hyperthermophile Pyrococcus furiosus
    M N Harris
    Department of Chemistry and Institute for Biomolecular Science, and the Department of Biology, University of South Florida, Tampa, Florida 33620, USA
    J Biol Chem 276:19310-7. 2001
    ..Such fundamental information is crucial to our understanding of the function of proteins at high temperature...
  84. ncbi Formation and stability of peptide enolates in aqueous solution
    Ana Rios
    Department of Chemistry, University at Buffalo, SUNY, Buffalo, New York 14260 3000, USA
    J Am Chem Soc 124:8251-9. 2002
    ....
  85. pmc Protein reorientation and bound water molecules measured by 1H magnetic spin-lattice relaxation
    Alexandra Van-Quynh
    Chemistry Department, University of Virginia, Charlottesville 22904 4319, USA
    Biophys J 84:558-63. 2003
    ....
  86. ncbi FTIR detection of water reactions during the flash-induced S-state cycle of the photosynthetic water-oxidizing complex
    Takumi Noguchi
    Institute of Materials Science, University of Tsukuba, Tsukuba, Ibaraki 305 8573, Japan
    Biochemistry 41:15706-12. 2002
    ..The results presented here showed that FTIR detection of water OH(D) bands can be a powerful method for investigating the mechanism of photosynthetic water oxidation...
  87. pmc Structure of poly(ethylene glycol)-modified horseradish peroxidase in organic solvents: infrared amide I spectral changes upon protein dehydration are largely caused by protein structural changes and not by water removal per se
    Wasfi Al-Azzam
    Department of Biology, University of Puerto Rico, San Juan, PR 00931, USA
    Biophys J 83:3637-51. 2002
    ..van de Weert, P.I. Haris, W.E. Hennink, and D.J. Crommelin. 2001. Anal. Biochem. 297:160-169). On the contrary, amide I IR spectral changes upon protein dehydration are caused by perturbations in the secondary structure...
  88. ncbi On-line determination of the deuterium abundance in breath water vapour by flowing afterglow mass spectrometry with applications to measurements of total body water
    D Smith
    Centre for Science and Technology in Medicine, School of Postgraduate Medicine, Keele University, Thornburrow Drive, Hartshill, Stoke-on-Trent ST4 7QB, UK
    Rapid Commun Mass Spectrom 15:25-32. 2001
    ..Thus, from the temporal variations of breath deuterium following the ingestion of a known quantity of D(2)O, we show that total body water can be determined non-invasively and the kinetics of water flow around the body can be tracked...
  89. ncbi Hydrogen bonding and catalysis of solvolysis of 4-methoxybenzyl fluoride
    Maria M Toteva
    Department of Chemistry, University at Buffalo, SUNY, Buffalo, New York 14260 3000, USA
    J Am Chem Soc 124:9798-805. 2002
    ..The decrease in the electronegativity of X along the series X = F, OH, Cl is accompanied by a ca. 10(10)-fold increase in the carbon basicity compared with the proton basicity of X-...
  90. ncbi Solvent and guest isotope effects on complexation thermodynamics of alpha-, beta-, and 6-amino-6-deoxy-beta-cyclodextrins
    Mikhail V Rekharsky
    Entropy Control Project, ICORP, JST, 4 6 3 Kamishinden, Toyonaka 560 0085, Japan
    J Am Chem Soc 124:12361-71. 2002
    ..9) of hydrogen oxide (H(2)O) or deuterium oxide (D(2)O) at 298.15 K by titration microcalorimetry...
  91. ncbi Secondary stabilization reactions and proton-coupled electron transport in photosystem II investigated by electroluminescence and fluorescence spectroscopy
    R de Wijn
    Department of Biophysics, Leiden University, P.O. Box 9504, 2300 RA Leiden, The Netherlands
    Biochemistry 40:5821-34. 2001
    ..The increased 'miss probability' in D2O is due to (iii)...
  92. ncbi FTIR detection of protonation/deprotonation of key carboxyl side chains caused by redox change of the Cu(A)-heme a moiety and ligand dissociation from the heme a3-Cu(B) center of bovine heart cytochrome c oxidase
    Daichi Okuno
    Department of Photoscience, The Graduate University for Advanced Studies, Center for Intergarative Bioscience, Okazaki National Research Institutes, Myodaiji, Okazaki, 444 8585, Japan
    J Am Chem Soc 125:7209-18. 2003
    ..Thus, the changes of carboxyl stretching bands induced both by oxidation of (Cu(A)Fe(a)()) and dissociation of CO appear at similar frequencies ( approximately 1749 cm(-)(1)) but are ascribed to different carboxyl side chains...
  93. ncbi A low temperature infrared study of the coordinated water in calcium malonate dihydrate
    E V Brusau
    Area de Química General e Inorgánica Dr Gabino F Puelles, Facultad de Quimica, Bioquimica y Farmacia, Universidad Nacional de San Luis, Argentina
    Spectrochim Acta A Mol Biomol Spectrosc 58:1769-74. 2002
    ..That result is indicative of a non-symmetric space group for the crystal, in agreement with one out of three different crystallographic studies made on the substance...
  94. ncbi Near infrared spectral investigations of hydration of 18-crown-6 in HDO-D2O solutions at different temperatures
    Kesharsingh J Patil
    Department of Chemistry, Shivaji University, Kolhapur, India
    Spectrochim Acta A Mol Biomol Spectrosc 59:1289-97. 2003
    ..Slightly different DeltaH degrees values obtained can be attributed to clathrate like structure at 1 m 18C6 concentration while at 2 m 18C6 concentration it is postulated that the hydrophobic interactions are contributing additionally...
  95. ncbi Deuterium structural effects in inorganic and bioinorganic aggregates
    Jaeho Lee
    Department of Chemistry, Brown University, Providence, Rhode Island 02912, USA
    J Am Chem Soc 124:3042-9. 2002
    ....
  96. ncbi Solvent isotope effect on the reaction catalysed by the pyruvate dehydrogenase complex from Escherichia coli
    Xiaoqing Liu
    Physiologisch Chemisches Institut, Universitat Tubingen, Hoppe Seyler Str 4, D 72076 Tubingen, Germany
    Biol Chem 384:673-9. 2003
    ..when its overall reaction or the partial reaction of the pyruvate dehydrogenase component were tested in deuterium oxide. The Michaelis constants for pyruvate were nearly unchanged, but the maximum velocities in water and ..
  97. pmc Cold denaturation and 2H2O stabilization of a staphylococcal nuclease mutant
    L C Antonino
    Department of Chemistry, University of California, Santa Cruz 95064
    Proc Natl Acad Sci U S A 88:7715-8. 1991
    ..When the stability of the mutant was examined in 2H2O by NMR, CD, or fluorescence, a substantial increase in the amount of folded protein at the tmax was noted as well as a decrease in tmax, reflecting increased stability...
  98. pmc Fast dynamics of halophilic malate dehydrogenase and BSA measured by neutron scattering under various solvent conditions influencing protein stability
    M Tehei
    Institut de Biologie Structurale, , 41 rue Jules Horowitz, F-38027 Grenoble Cedex 1, France
    Proc Natl Acad Sci U S A 98:14356-61. 2001
    ..From previous data, it is suggested that these are associated with hydrated ion binding stabilizing the protein in the high-salt solvent...
  99. ncbi Total body dual X-ray absorptiometry is a good measure of both fat mass and fat-free mass in liver cirrhosis compared to "gold-standard" techniques. Melbourne Liver Group
    B J Strauss
    Body Composition Laboratory, Monash Medical Centre, Victoria, Australia
    Ann N Y Acad Sci 904:55-62. 2000
    ..73, but women were significantly overhydrated compared to men. Relative to the gold-standard deuterium oxide dilution method for measuring total body water, SFBIA slightly overestimated TBW, whereas MFBIA slightly ..
  100. ncbi Fluid status, blood pressure, and cardiovascular abnormalities in patients on peritoneal dialysis
    Constantijn J A M Konings
    Department of Internal Medicine and Nephrology, Academic Hospital Maastricht, The Netherlands
    Perit Dial Int 22:477-87. 2002
    ....
  101. ncbi Lipoprotein lipase activity and its relationship to high milk fat transfer during lactation in grey seals
    S J Iverson
    Canadian Institute of Fisheries Technology, Technical University of Nova Scotia, Halifax, Canada
    J Comp Physiol B 165:384-95. 1995
    ..We measured pup body composition and milk fat intake by isotope (deuterium oxide) dilution and plasma post-heparin lipoprotein lipase activity in six grey seal (Halichoerus grypus) mother-..

Research Grants41

  1. In vivo assessment of T cell kinetics in individuals at risk for Type 1 diabetes
    Carla J Greenbaum; Fiscal Year: 2013
    ..Advances in stable isotope methodologies (labeling with deuterium oxide (2H2O) and deuterated glucose (2H2-glucose) are used to measure the kinetics of cell populations in vivo in ..
  2. Chemical Structure Effects on Bone Response to Mechanical Load
    MICHAEL DAVID MORRIS; Fiscal Year: 2013
    ..disordering of the collagen and mineral by partial and complete displacement of native matrix water with deuterium oxide, which forms weaker hydrogen bonds...
  3. Role of 5-alpha Reductase in Testosterone Actions
    Shalendar Bhasin; Fiscal Year: 2007
    ..The following outcomes will be measured at baseline and after 20 weeks: body composition by DEXA scan, deuterium oxide and sodium bromide dilution; thigh muscle volume by MRI scan; muscle performance by measurements of 1-..
  4. ART PHARMACOKINETICS IN SEVERELY MALNOURISHED HIV-INFECTED CHILDREN
    Ericka Hayes; Fiscal Year: 2009
    ..to be studied at baseline and 2 and 8 weeks after starting HAART include lean body mass (calculated by deuterium oxide dilution method), mitochondrial integrity (measured by lactate and copy numbers of mitochondrial DNA/RNA in ..
  5. Testosterone:Physical Function in Men on Hemodialysis
    Shalender Bhasin; Fiscal Year: 2004
    ..The following outcomes will be measured at baseline and after 16 weeks: body composition by DEXA scan, deuterium oxide and sodium bromide dilution; thigh muscle volume by MRI scan; muscle performance by measurements of 5-..
  6. FAT AND FAT-FREE BODY COMPOSITION DEVELOPMENT IN AGING
    Richard Boileau; Fiscal Year: 1992
    ..approach will be utilized involving measures of density (hydrostatic weighing), total body water (deuterium oxide dilution, bone mineral content (photon absorptiometry), and body potassium (40K count)...
  7. BODY COMPOSITION AND NUTRITION IN SPINAL CORD INJURY
    PAUL SCHLOERB; Fiscal Year: 1991
    ..Total body water will be measured with deuterium oxide (heavy water) and bioimpedance and extracellular fluid will be determined with bromide...
  8. Retinol Equivalence of Plant Carotenoids in Children
    Guangwen Tang; Fiscal Year: 2006
    ..This study will be of importance in planning vitamin A deficiency prevention strategies and also will provide useful information regarding the potential efficacy of a bioengineered crop to provide vitamin A nutrition. ..
  9. ADIPOCYTE TURNOVER AND METABOLISM IN HIV-1 LIPOATROPHY
    Marc Hellerstein; Fiscal Year: 2003
    ....
  10. T CELL KINETICS AND SOURCES--EFFECTS OF HIV AND THERAPY
    Marc Hellerstein; Fiscal Year: 2002
    ....
  11. Response to and signals of caloric restriction and intermittent feeding regimens
    MARC KOPEL HELLERSTEIN; Fiscal Year: 2010
    ....
  12. DIRECTLY MEASURED T CELL DYNAMICS IN HIV PATHOGENESIS
    Marc Hellerstein; Fiscal Year: 2004
    ..Results have potential relevance to HIV-1 pathogenesis and therapy and to vaccination strategies. ..
  13. Kinetic Biomarker for CLL Prognosis
    Scott Turner; Fiscal Year: 2007
    ..abstract_text> ..
  14. Epitope mapping by H/D exchange mass spectrometry
    Yoshitomo Hamuro; Fiscal Year: 2008
    ..Establishing a fast, cheap and reliable methodology for the epitope mapping should have a direct impact on the monoclonal antibody therapeutic business. [unreadable] [unreadable] [unreadable]..
  15. An instrument to measure protein amide H/D exchange within a second
    Yoshitomo Hamuro; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  16. Immuno-modulatory Effects of Parenteral Nutrition After Intestinal Failure
    Stephen Benedict; Fiscal Year: 2007
    ..The data obtained will position the research team to develop more mechanistically in-depth RO1 proposals that investigate human patients and a murine PN animal model. [unreadable] [unreadable] [unreadable]..
  17. An in Vivo Neurogenesis Assay to Discover Antidepressants and Cognitive Enhancers
    Mahalakshmi Shankaran; Fiscal Year: 2008
    ..We will use a powerful new method for measuring the production of new brain cells as a way to discover new drugs, and we will then test the effectiveness of these drugs on depression and memory. [unreadable] [unreadable] [unreadable]..
  18. NONINVASIVE MEASUREMENT OF ENERGY EXPENDITURE IN HUMANS
    Dale Schoeller; Fiscal Year: 2007
    ..These studies will provide important guidelines for the prescription of exercise for the prevention and treatment of obesity. ..
  19. Exploring Growth Energy Expenditure in Normal-Weight and Overweight Children
    ZiMian Wang; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  20. MALNUTRITION IN DIALYSIS PATIENTS
    TALAT IKIZLER; Fiscal Year: 2006
    ..abstract_text> ..
  21. KINETIC CONTROL IN THIOREDOXINS AND DISULFIDE ISOMERASES
    DAVID LEMASTER; Fiscal Year: 2001
    ..abstract_text> ..
  22. NEUTROPHINS AND RETINAL DEVELOPMENT
    Janet Sparrow; Fiscal Year: 2001
    ..The use of neurotrophins in conjunction with gene therapy or transplantation may also have potential for reversing retinal degenerative changes and lead to the restoration of vision. ..
  23. Metabolic Adapation and Control of Lactation in Humans
    Morey W Haymond; Fiscal Year: 2010
    ....
  24. MECHANISMS OF HYPOALBUMINEMIA IN ENDSTAGE RENAL DISEASE
    George Kaysen; Fiscal Year: 2001
    ..We will further establish the effect of KT/V, dialyzer permeability and reuse on changes in albumin, nutritional status and acute phase response. ..
  25. Baylor Mentored Clinical Investigator Award
    Morey Haymond; Fiscal Year: 2006
    ..The funding of this program is crucial to the success in training academic physicians with independent peer reviewed research funding. [unreadable] [unreadable] [unreadable]..
  26. Stereochemistry of Elimination and Proton Transfer
    JERRY MOHRIG; Fiscal Year: 2002
    ..Carleton College undergraduate chemistry majors will carry out the research. The principal investigator has had substantial experience in crafting successful undergraduate research experiences over his teaching career. ..
  27. MECHANISM, SPECIFICITY & REGULATION: CDC25 PHOSPHATASES
    Johannes Rudolph; Fiscal Year: 2004
    ..Thus, this research will greatly expand the knowledge of cell cycle transitions, their control on a molecular basis, and their role in oncogenesis. ..
  28. ISOTOPE RATIO MASS SPECTROMETER
    Dale Schoeller; Fiscal Year: 2006
    ..In addition, this new instrument will provide new capacity in the form of the solids combustion inlet. This expands our potential users group in the area of development of dietary biomarkers. [unreadable] [unreadable]..
  29. PROTON LEAK, OXIDATIVE STRESS, AND ENERGY RESTRICTION
    Jon Ramsey; Fiscal Year: 2003
    ..The premise is that Caloric Restriction (CR), hypothyroidism, and modulation of dietary fat will decrease mitochondrial permeability (i.e. proton leak), therefore decreasing molecular Oxygen consumption, and presumably oxidative stress. ..
  30. NMR STUDIES:HEPATIC GLUCOSE METABOL IN HUMANS EXERCISING
    Kitt Petersen; Fiscal Year: 2003
    ..The results of these studies should provide important new insight into the regulation of hepatic glucose metabolism in healthy and type 1 diabetic subjects. ..
  31. MR elastography using time reversed acoustics
    Scott Swanson; Fiscal Year: 2004
    ..3) Integrate the MR compatible TRA system with the MRI system and demonstrate the principle and the feasibility of the MR SWEI system based on TRA. ..
  32. TRIAL OF ACTIVITY FOR ADOLESCENT GIRLS (TAAG)
    Timothy Lohman; Fiscal Year: 2005
    ..The team brings expertise in PA program design, PA and fitness assessment, formative assessment, behavior theory and curriculum development, intervention delivery, process evaluation, and data management. ..
  33. How Substrates Activate Fumarase and Glyoxalase Evolved
    Irwin Rose; Fiscal Year: 2005
    ..Since methylglyoxal is toxic to cells and must be removed by an active glyoxalse system, inhibition of a MSH based system could result in a treatment for many mycobacterial-based diseases such as tuberculosis. ..
  34. PRESERVATION OF GENETIC LINES IN A NON MAMMALIAN SYSTEM
    Mary Hagedorn; Fiscal Year: 2005
    ..Most importantly, these somatic changes will not affect the germ line, so they are ideal for genomic banking to maintain important genetic stocks for biomedical and endangered fish research. ..
  35. A Novel Biomarker for Myelin Synthesis
    Elizabeth Murphy; Fiscal Year: 2005
    ..In phase II we plan to apply the plasma GalC kinetic biomarker in patients with MS to investigate aspects of disease activity and response to therapeutic interventions. ..
  36. CLINICAL SCIENTIST TRAINING PROGRAM
    Morey Haymond; Fiscal Year: 2004
    ..The awarding of this grant will dramatically accelerate our implementation of this program. ..
  37. Genetic Analyzer
    Morey Haymond; Fiscal Year: 2002
    ..In total, we anticipate a demand for approximately 71,500 samples in the first year of operating the new instrument. ..
  38. Baylor Mentored Diabetes Investigator Award
    Morey Haymond; Fiscal Year: 2005
    ..The funding of this program is crucial to our success in training academic physicians with independent peer reviewed research funding. ..
  39. LC-MS/MS Methods for Analysis of Hemoglogbin Adducts
    Timothy Fennell; Fiscal Year: 2004
    ..abstract_text> ..
  40. PROTEIN NMR STRUCTURE AND DYNAMICS VIA ISOTOPIC LABELING
    DAVID LEMASTER; Fiscal Year: 2002
    ..This labeling pattern is likewise well suited for more conventional NOE-based solution structure determinations and its range of utility will be demonstrated ..
  41. ABI PRISM 7900HT SDS 96-Well Sequence Detection System
    Morey Haymond; Fiscal Year: 2006
    ..Partha Sen with administrative oversight from Dr. Morey Haymond, Program Director, CHRC and Vice Chair for Res. for the Dept of Pediatrics, Baylor Coll. of Medicine. [unreadable] [unreadable] [unreadable]..