spin labels

Summary

Summary: Molecules which contain an atom or a group of atoms exhibiting an unpaired electron spin that can be detected by electron spin resonance spectroscopy and can be bonded to another molecule. (McGraw-Hill Dictionary of Chemical and Technical Terms, 4th ed)

Top Publications

  1. pmc Continuous flow-driven inversion for arterial spin labeling using pulsed radio frequency and gradient fields
    Weiying Dai
    Department of Radiology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    Magn Reson Med 60:1488-97. 2008
  2. ncbi A general kinetic model for quantitative perfusion imaging with arterial spin labeling
    R B Buxton
    Department of Radiology, University of San Diego, California, USA
    Magn Reson Med 40:383-96. 1998
  3. ncbi Distance measurements on spin-labelled biomacromolecules by pulsed electron paramagnetic resonance
    Gunnar Jeschke
    University of Konstanz, Universitätsstrasse, 78457 Konstanz, Germany
    Phys Chem Chem Phys 9:1895-910. 2007
  4. ncbi Single-shot 3D imaging techniques improve arterial spin labeling perfusion measurements
    Matthias Gunther
    Advanced MRI Technologies, Sebastopol, California 95472, USA
    Magn Reson Med 54:491-8. 2005
  5. ncbi Can arterial spin labeling detect white matter perfusion signal?
    Matthias J P van Osch
    Department of Radiology, Leiden University Medical Center, Leiden, The Netherlands
    Magn Reson Med 62:165-73. 2009
  6. ncbi Separation of macrovascular signal in multi-inversion time arterial spin labelling MRI
    Michael A Chappell
    Centre for Functional MRI of the Brain, University of Oxford, John Radcliffe Hospital, Headington, Oxford, UK
    Magn Reson Med 63:1357-65. 2010
  7. ncbi Rotamer libraries of spin labelled cysteines for protein studies
    Yevhen Polyhach
    Laboratory of Physical Chemistry, ETH Zurich, Wolfgang Pauli Str 10, 8093 Zurich, Switzerland
    Phys Chem Chem Phys 13:2356-66. 2011
  8. pmc Pattern of cerebral hypoperfusion in Alzheimer disease and mild cognitive impairment measured with arterial spin-labeling MR imaging: initial experience
    Nathan A Johnson
    MR Research Unit, VA Medical Center, 4150 Clement St 114M San Francisco, CA 94107, USA
    Radiology 234:851-9. 2005
  9. pmc Chemistry and antihypertensive effects of tempol and other nitroxides
    Christopher S Wilcox
    Division of Nephrology and Hypertension, Kidney and Vascular Disorder Center, Georgetown University, Washington, DC 20007, USA
    Pharmacol Rev 60:418-69. 2008
  10. ncbi Arterial spin-labeled perfusion MRI in basic and clinical neuroscience
    John A Detre
    Department of Neurology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Curr Opin Neurol 22:348-55. 2009

Research Grants

  1. Structural Dynamics of Cardiac Muscle Contraction
    Brett A Colson; Fiscal Year: 2012
  2. Structure of the GABA-A Receptor Binding Sites
    CYNTHIA M CZAJKOWSKI; Fiscal Year: 2013
  3. Structure of the GABA-A Receptor Binding Sites
    CYNTHIA M CZAJKOWSKI; Fiscal Year: 2012
  4. STRUCTURAL ANALYSIS OF THE MANNOSE 6-PHOSPHATE RECEPTORS
    Nancy M Dahms; Fiscal Year: 2013
  5. Structural Dynamics of Multi-drug Transporters
    Hassane S McHaourab; Fiscal Year: 2013
  6. Structural Dynamics of Cardiac Calcium Transport Regulation
    NAA ADJELEY DROMOH ABLORH; Fiscal Year: 2013
  7. PROTEIN FOLDING &UNFOLDING BY DQC CYTOCHROME C
    Jack H Freed; Fiscal Year: 2011
  8. Reactive Intermediates in Lipoxygenase Pathways
    Betty J Gaffney; Fiscal Year: 2012
  9. Development of Biomedical EPR Instrumentation
    James S Hyde; Fiscal Year: 2013
  10. Pulsed Q-band EPR Spectrometer
    Albert H Beth; Fiscal Year: 2010

Detail Information

Publications364 found, 100 shown here

  1. pmc Continuous flow-driven inversion for arterial spin labeling using pulsed radio frequency and gradient fields
    Weiying Dai
    Department of Radiology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    Magn Reson Med 60:1488-97. 2008
    ..Imaging at 3T with body coil transmission was readily achieved. This technique should help to realize the benefits of continuous labeling in clinical imagers...
  2. ncbi A general kinetic model for quantitative perfusion imaging with arterial spin labeling
    R B Buxton
    Department of Radiology, University of San Diego, California, USA
    Magn Reson Med 40:383-96. 1998
    ..In preliminary experiments with a human subject, the model provided a good description of pulsed ASL data during a simple sensorimotor activation task...
  3. ncbi Distance measurements on spin-labelled biomacromolecules by pulsed electron paramagnetic resonance
    Gunnar Jeschke
    University of Konstanz, Universitätsstrasse, 78457 Konstanz, Germany
    Phys Chem Chem Phys 9:1895-910. 2007
    ..This length scale can be accessed by distance measurements between spin labels if pulsed electron paramagnetic resonance (EPR) techniques such as electron-electron double resonance (ELDOR) ..
  4. ncbi Single-shot 3D imaging techniques improve arterial spin labeling perfusion measurements
    Matthias Gunther
    Advanced MRI Technologies, Sebastopol, California 95472, USA
    Magn Reson Med 54:491-8. 2005
    ..For arterial spin labeling, single-shot 3D imaging techniques are optimal in terms of efficiency and might prove beneficial to improve reliability of perfusion quantitation in a clinical setup...
  5. ncbi Can arterial spin labeling detect white matter perfusion signal?
    Matthias J P van Osch
    Department of Radiology, Leiden University Medical Center, Leiden, The Netherlands
    Magn Reson Med 62:165-73. 2009
    ..It can be concluded that, except within the deep WM, ASL is sensitive enough to detect WM perfusion signal and perfusion deficits...
  6. ncbi Separation of macrovascular signal in multi-inversion time arterial spin labelling MRI
    Michael A Chappell
    Centre for Functional MRI of the Brain, University of Oxford, John Radcliffe Hospital, Headington, Oxford, UK
    Magn Reson Med 63:1357-65. 2010
    ....
  7. ncbi Rotamer libraries of spin labelled cysteines for protein studies
    Yevhen Polyhach
    Laboratory of Physical Chemistry, ETH Zurich, Wolfgang Pauli Str 10, 8093 Zurich, Switzerland
    Phys Chem Chem Phys 13:2356-66. 2011
    ..Suggestions for the selection of labelling sites in proteins are given, limitations of the approach discussed, and requirements for further development are outlined...
  8. pmc Pattern of cerebral hypoperfusion in Alzheimer disease and mild cognitive impairment measured with arterial spin-labeling MR imaging: initial experience
    Nathan A Johnson
    MR Research Unit, VA Medical Center, 4150 Clement St 114M San Francisco, CA 94107, USA
    Radiology 234:851-9. 2005
    ....
  9. pmc Chemistry and antihypertensive effects of tempol and other nitroxides
    Christopher S Wilcox
    Division of Nephrology and Hypertension, Kidney and Vascular Disorder Center, Georgetown University, Washington, DC 20007, USA
    Pharmacol Rev 60:418-69. 2008
    ..Thus, tempol is broadly effective in reducing blood pressure, whether given by acute intravenous injection or by prolonged administration, in a wide range of rodent models of hypertension...
  10. ncbi Arterial spin-labeled perfusion MRI in basic and clinical neuroscience
    John A Detre
    Department of Neurology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Curr Opin Neurol 22:348-55. 2009
    ....
  11. ncbi Arterial spin labeling in combination with a look-locker sampling strategy: inflow turbo-sampling EPI-FAIR (ITS-FAIR)
    M Gunther
    Deutsches Krebsforschungszentrum DKFZ, Forschungsschwerpunkt Radiologische Diagnostik und Therapie E0201, Heidelberg, Germany
    Magn Reson Med 46:974-84. 2001
    ..With the ability to obtain ITS-FAIR time series with high temporal resolution arterial transit times in the range of -138 - 1054 ms were measured, where nonphysical negative values were found in voxels containing large vessels...
  12. ncbi Protein structure determination using long-distance constraints from double-quantum coherence ESR: study of T4 lysozyme
    Petr P Borbat
    Baker Laboratory of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853 1301, USA
    J Am Chem Soc 124:5304-14. 2002
    ..This study sets the stage for the use of DQC-ESR for determining the tertiary structure of large proteins with just a small number of long-distance constraints...
  13. ncbi The determination of pair distance distributions by pulsed ESR using Tikhonov regularization
    Yun Wei Chiang
    Baker Laboratory of Chemistry and Chemical Biology, National Biomedical ACERT Center for Advanced ESR Technology, Cornell University, Ithaca, NY 14853 1301, USA
    J Magn Reson 172:279-95. 2005
    ..This study supplies a mathematically reliable method for extracting pair distributions from pulsed ESR experimental data and has extended the use of pulsed ESR to provide results of greater value for structural biology...
  14. ncbi Model-free arterial spin labeling quantification approach for perfusion MRI
    Esben Thade Petersen
    Department of Neuroradiology, National Neuroscience Institute, Singapore
    Magn Reson Med 55:219-32. 2006
    ..Monte Carlo simulations were performed to compare this new methodology with parametric fitting by the conventional model...
  15. pmc Effect of freezing conditions on distances and their distributions derived from Double Electron Electron Resonance (DEER): a study of doubly-spin-labeled T4 lysozyme
    Elka R Georgieva
    Department of Chemistry and Chemical Biology and ACERT, Cornell University, Ithaca, NY 14853, United States
    J Magn Reson 216:69-77. 2012
    ..The assignment of bimodal distributions to tether rotamers as opposed to protein conformations is aided by comparing results using MTSL and 4-Bromo MTSL spin-labels. The latter usually produce narrower distance distributions...
  16. ncbi Long-range distance determinations in biomacromolecules by EPR spectroscopy
    Olav Schiemann
    Institute of Physical and Theoretical Chemistry, Center for Biomolecular Magnetic Resonance, J W Goethe University Frankfurt, 60438 Frankfurt am Main, Germany
    Q Rev Biophys 40:1-53. 2007
    ..Spin centers may be metal ions, metal clusters, cofactor radicals, amino acid radicals, or spin labels. In this review, we discuss the advantages and limitations of the different EPR spectroscopic methods, briefly ..
  17. ncbi Measuring the effects of remifentanil on cerebral blood flow and arterial arrival time using 3D GRASE MRI with pulsed arterial spin labelling
    Bradley J MacIntosh
    FMRIB Centre, Department of Clinical Neurology, University of Oxford, Oxford, UK
    J Cereb Blood Flow Metab 28:1514-22. 2008
    ..7+/-1.60 %CBF per mm Hg. Significant differences between physiologic conditions were observed in both CBF and AAT maps, indicating that 3D GRASE-ASL has the sensitivity to study changes in physiology at a voxel level...
  18. ncbi Efficiency of inversion pulses for background suppressed arterial spin labeling
    Dairon M Garcia
    Department of Radiology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    Magn Reson Med 54:366-72. 2005
    ..Knowledge of efficiency will aid optimization of the number of suppression pulses and provide more accurate quantification of blood flow...
  19. ncbi A theoretical and experimental investigation of the tagging efficiency of pseudocontinuous arterial spin labeling
    Wen Chau Wu
    Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Magn Reson Med 58:1020-7. 2007
    ..These improvements provide a better balance between tagging efficiency and SNR...
  20. ncbi Multislice perfusion of the kidneys using parallel imaging: image acquisition and analysis strategies
    Alexander G Gardener
    Sir Peter Mansfield Magnetic Resonance Centre, School of Physics and Astronomy, University of Nottingham, Nottingham, UK
    Magn Reson Med 63:1627-36. 2010
    ..The addition of background suppression to arterial spin labeling data is shown to reduce the perfusion signal-to-noise ratio and underestimate perfusion...
  21. ncbi Arterial spin labeling perfusion fMRI with very low task frequency
    Jiongjiong Wang
    Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Magn Reson Med 49:796-802. 2003
    ..The improved sensitivity of the ASL contrast for low task frequency and longitudinal studies, along with its superior power in group analysis, is expected to extend the range of experimental designs that can be studied using fMRI...
  22. ncbi A general framework for the analysis of vessel encoded arterial spin labeling for vascular territory mapping
    Michael A Chappell
    Oxford Centre for Functional MRI of the Brain, University of Oxford, John Radcliffe Hospital, Headington, Oxford, United Kingdom
    Magn Reson Med 64:1529-39. 2010
    ..Similar performance was found for data acquired using a pseudo-continuous labeling scheme both in the neck and above the Circle of Willis...
  23. pmc Time-resolved vessel-selective digital subtraction MR angiography of the cerebral vasculature with arterial spin labeling
    Philip M Robson
    Department of Radiology, Beth Israel Deaconess Medical Center and Harvard Medical School, 330 Brookline Ave, Ansin 242, Boston, MA 02215, USA
    Radiology 257:507-15. 2010
    ....
  24. ncbi Velocity-selective arterial spin labeling
    Eric C Wong
    Department of Radiology, University of California San Diego, La Jolla, 92093, USA
    Magn Reson Med 55:1334-41. 2006
    ..The technical considerations described here include the design of VS pulses, background suppression, anisotropy with respect to velocity-encoding directions, and CBF quantitation issues...
  25. pmc Arterial spin labeling MRI for assessment of perfusion in native and transplanted kidneys
    Nathan S Artz
    Department of Medical Physics, University of Wisconsin Madison, Wisconsin Institute for Medical Research, Madison, WI 53705 2275, USA
    Magn Reson Imaging 29:74-82. 2011
    ..To apply a magnetic resonance arterial spin labeling (ASL) technique to evaluate kidney perfusion in native and transplanted kidneys...
  26. pmc Improved arterial spin labeling after myocardial infarction in mice using cardiac and respiratory gated look-locker imaging with fuzzy C-means clustering
    Moriel H Vandsburger
    Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia 22908, USA
    Magn Reson Med 63:648-57. 2010
    ..This technique, when applied to genetically engineered mice, will allow for the investigation of the roles of specific genes in myocardial perfusion during infarct healing...
  27. ncbi Quantitation of renal perfusion using arterial spin labeling with FAIR-UFLARE
    N Karger
    Klinikum an der CAU zu Kiel, Klinik für Diagnostische Radiologie, Michaelisstr 9, 24105, Kiel, Germany
    Magn Reson Imaging 18:641-7. 2000
    ....
  28. ncbi Methods and applications of site-directed spin labeling EPR spectroscopy
    Candice S Klug
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Methods Cell Biol 84:617-58. 2008
    ..this technique can detect changes in distances between two sites and provide information on the depth of spin labels located within a membrane bilayer...
  29. pmc Global structure of a three-way junction in a phi29 packaging RNA dimer determined using site-directed spin labeling
    Xiaojun Zhang
    Department of Chemistry, University of Southern California, Los Angeles, California 90089, USA
    J Am Chem Soc 134:2644-52. 2012
    ....
  30. ncbi Renal perfusion 3-T MR imaging: a comparative study of arterial spin labeling and dynamic contrast-enhanced techniques
    Wen Chau Wu
    Graduate Institute of Oncology, National Taiwan University, No 1, Sec 1, Ren Ai Rd, Taipei 100, Taiwan
    Radiology 261:845-53. 2011
    ..To investigate the feasibility of and correlation between arterial spin-labeling (ASL) and dynamic contrast material-enhanced (DCE) 3-T magnetic resonance (MR) imaging in the measurement of renal blood flow (RBF)...
  31. pmc Transmembrane gate movements in the type II ATP-binding cassette (ABC) importer BtuCD-F during nucleotide cycle
    Benesh Joseph
    Laboratory of Physical Chemistry, ETH Zurich, Wolfgang Pauli Strasse 10, 8093 Zurich, Switzerland
    J Biol Chem 286:41008-17. 2011
    ..The EPR data allow a productive translocation cycle of the vitamin B(12) transporter to be modeled...
  32. ncbi Pulsed star labeling of arterial regions (PULSAR): a robust regional perfusion technique for high field imaging
    Xavier Golay
    Department of Neuroradiology, National Neuroscience Institute, 11 Jalan Tan Tock Seng, Singapore 308433
    Magn Reson Med 53:15-21. 2005
    ....
  33. ncbi Estimation of inter-residue distances in spin labeled proteins at physiological temperatures: experimental strategies and practical limitations
    C Altenbach
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-7008, USA
    Biochemistry 40:15471-82. 2001
    ..The existence of such distance distributions places important constraints on the interpretation of internitroxide distances in terms of protein structure and structural changes...
  34. pmc Computer modeling of nitroxide spin labels on proteins
    Ma mon M Hatmal
    Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA 90089, USA
    Biopolymers 97:35-44. 2012
    ..5 Å. The speed and accuracy of PRONOX suggest that the algorithm can be used for fitting to DEER data for determination of protein tertiary structure...
  35. ncbi Quantification of renal perfusion: comparison of arterial spin labeling and dynamic contrast-enhanced MRI
    Jeff D Winter
    Research Institute, Diagnostic Imaging, Hospital for Sick Children, Toronto, Canada
    J Magn Reson Imaging 34:608-15. 2011
    ..Moreover, we provide the first application of the dual bolus approach to quantitative DCE-MRI perfusion measurements in the kidney...
  36. ncbi Modeling the effects of dispersion and pulsatility of blood flow in pulsed arterial spin labeling
    Daniel Gallichan
    FMRIB Centre, John Radcliffe Hospital, University of Oxford, Oxford, UK
    Magn Reson Med 60:53-63. 2008
    ..Effects due to pulsatility are expected to be observable, but when no cardiac-gating is used the mean curve over several cardiac cycles is predicted to closely match the curve which assumes constant flow...
  37. ncbi Pediatric perfusion imaging using pulsed arterial spin labeling
    Jiongjiong Wang
    Department of Radiology, University of Pennsylvania, Philadelphia 19104, USA
    J Magn Reson Imaging 18:404-13. 2003
    ..To test the feasibility of pediatric perfusion imaging using a pulsed arterial spin labeling (ASL) technique at 1.5 T...
  38. pmc Opposite movement of the external gate of a glutamate transporter homolog upon binding cotransported sodium compared with substrate
    Paul J Focke
    Neuroscience Graduate Program, School of Medicine, Oregon Health and Science University, Beaverton, Oregon 97006 8921, USA
    J Neurosci 31:6255-62. 2011
    ....
  39. pmc Structural refinement from restrained-ensemble simulations based on EPR/DEER data: application to T4 lysozyme
    Shahidul M Islam
    Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois 60637, United States
    J Phys Chem B 117:4740-54. 2013
    ..This computationally efficient approach allows experimental restraints from DEER experiments to be incorporated into RE simulations for efficient structural refinement...
  40. ncbi Spin label EPR-based characterization of biosystem complexity
    Janez Strancar
    Laboratory of Biophysics, Jozef Stefan Institute, Jamova 39, SI 1000 Ljubljana, Slovenia
    J Chem Inf Model 45:394-406. 2005
    ....
  41. pmc Assessing oligomerization of membrane proteins by four-pulse DEER: pH-dependent dimerization of NhaA Na+/H+ antiporter of E. coli
    Daniel Hilger
    Ludwig Maximilians Universitat Munchen, Department Biologie I, Germany
    Biophys J 89:1328-38. 2005
    ....
  42. ncbi Maximum entropy: a complement to Tikhonov regularization for determination of pair distance distributions by pulsed ESR
    Yun Wei Chiang
    Baker Laboratory of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853 1301, USA
    J Magn Reson 177:184-96. 2005
    ..Thus we regard the MEM method as a complement to TIKR by securing a positive pair distance distribution and enhancing the accuracy of TIKR...
  43. ncbi Measuring distances by pulsed dipolar ESR spectroscopy: spin-labeled histidine kinases
    Peter P Borbat
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY, USA
    Methods Enzymol 423:52-116. 2007
    ....
  44. ncbi Normal cerebral perfusion measurements using arterial spin labeling: reproducibility, stability, and age and gender effects
    Laura M Parkes
    Department of Neuroinflammation, Institute of Neurology, University College London, London, UK
    Magn Reson Med 51:736-43. 2004
    ..45% per year (P = 0.04). Regional analysis suggested that the gray-matter age-related changes were predominantly localized in the frontal cortex. Whole-brain perfusion was 13% higher (P = 0.02) in females compared to males...
  45. ncbi Cancer chemoprevention by the antioxidant tempol in Atm-deficient mice
    Ralf Schubert
    Department of Pediatrics, The Comprehensive Cancer Center, UCSD School of Medicine, La Jolla, CA 92093 0627, USA
    Hum Mol Genet 13:1793-802. 2004
    ..Thus, tempol acts as a novel chemopreventative agent in this mouse model of a human cancer prone syndrome, associated with broad antioxidant effects...
  46. ncbi Non-invasive measurement of perfusion: a critical review of arterial spin labelling techniques
    E T Petersen
    Department of Neuroradiology, National Neuroscience Institute, Singapore
    Br J Radiol 79:688-701. 2006
    ..In particular, the perfusion quantification methods, including the problems and pitfalls involved, are thoroughly discussed in this article. Finally, a brief summary of applications is provided...
  47. pmc Test-retest reliability of arterial spin labeling with common labeling strategies
    Yufen Chen
    Department of Neurology, University of Pennsylvania, Philadelphia, PA, USA
    J Magn Reson Imaging 33:940-9. 2011
    ..To compare the test-retest reproducibility of three variants of arterial spin labeling (ASL): pseudo-continuous (pCASL), pulsed (PASL) and continuous (CASL)...
  48. ncbi Arterial transit time imaging with flow encoding arterial spin tagging (FEAST)
    Jiongjiong Wang
    Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Magn Reson Med 50:599-607. 2003
    ..Application of the FEAST technique in two patients with cerebrovascular disease demonstrated prolonged tissue transit times in the affected vascular territories which were consistent with results from other MR imaging modalities...
  49. ncbi Spin labeling EPR
    Johann P Klare
    Physics Department, University of Osnabruck, Barbarastr 7, 49076, Osnabruck, Germany
    Photosynth Res 102:377-90. 2009
    ....
  50. ncbi Quantification of pain-induced changes in cerebral blood flow by perfusion MRI
    D G Owen
    Lawson Health Research Institute, St Joseph s Health Care London, 268 Grosvenor Street, London, Ont, Canada N6A 4V2
    Pain 136:85-96. 2008
    ..97ml/100g/min in ipsilateral thalamus to 4.91ml/100g/min in contralateral insula. The average resting global CBF was 54+/-9.7ml/100g/min, and there was no change in global CBF due to the noxious thermal stimulus...
  51. ncbi MR-determined metabolic phenotype of breast cancer in prediction of lymphatic spread, grade, and hormone status
    Tone F Bathen
    Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, 7489 Trondheim, Norway
    Breast Cancer Res Treat 104:181-9. 2007
    ..MR-determined metabolic phenotype may have a future role as a supplement for clinical decision-making-concerning adjuvant treatment and the adaptation to more individualised treatment protocols...
  52. pmc The QUASAR reproducibility study, Part II: Results from a multi-center Arterial Spin Labeling test-retest study
    Esben Thade Petersen
    Center of Functionally Integrative Neuroscience, Aarhus University Hospital, Nørrebrogade 44, Aarhus C, Denmark
    Neuroimage 49:104-13. 2010
    ..5 [ml/100 g/min] and a within-subject standard deviation SD(w)=4.7 [ml/100 g/min]. The corresponding repeatability was 13.0 [ml/100 g/min] and was found to be within the range of previous studies...
  53. ncbi TEMPOL, a membrane-permeable radical scavenger, attenuates peroxynitrite- and superoxide anion-enhanced carrageenan-induced paw edema and hyperalgesia: a key role for superoxide anion
    Mahmoud M Khattab
    Department of Pharmacology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
    Eur J Pharmacol 548:167-73. 2006
    ..This raises the chances for considering pharmacologic interventions that interrupt superoxide anion and peroxynitrite stress for putative alternative agents as anti-inflammatory analgesic new medical strategies...
  54. ncbi Identifying conformational changes with site-directed spin labeling
    W L Hubbell
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, USA
    Nat Struct Biol 7:735-9. 2000
    ..changes in the mobility and solvent accessibility of the spin label side chains, and in the distances between spin labels, report (i) rigid body motions of alpha-helices and beta-strands (ii) relative movements of domains and (iii) ..
  55. pmc Increased sensitivity and extended range of distance measurements in spin-labeled membrane proteins: Q-band double electron-electron resonance and nanoscale bilayers
    Ping Zou
    Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee
    Biophys J 98:L18-20. 2010
    ..The advances described here set the stage for the use of DEER spectroscopy to analyze conformational dynamics of sample-limited eukaryotic membrane proteins...
  56. ncbi Electron paramagnetic resonance spectroscopy of site-directed spin labels reveals the structural heterogeneity in the N-terminal domain of apoA-I in solution
    Jens O Lagerstedt
    Department of Biochemistry, University of California, Davis, California 95616, USA
    J Biol Chem 282:9143-9. 2007
    ..Using electron paramagnetic spectroscopy of site-directed spin labels in the N-terminal domain of apoA-I (residues 1-98) we have mapped a mixture of secondary structural elements, ..
  57. pmc Lesion-induced DNA weak structural changes detected by pulsed EPR spectroscopy combined with site-directed spin labelling
    Giuseppe Sicoli
    Laboratoire de Résonance Magnétique, UMR E no 3 CEA UJF FRE 3200 CNRS Institut des Nanosciences et Cryogénie, CEA Grenoble, 17, avenue des Martyrs, F 38054, Grenoble Cedex 9, France
    Nucleic Acids Res 37:3165-76. 2009
    ..Thus, the experimental approaches described herein provide an efficient method for probing lesion-induced structural changes of nucleic acids...
  58. pmc Pseudocontinuous arterial spin labeling reveals dissociable effects of morphine and alcohol on regional cerebral blood flow
    Najmeh Khalili-Mahani
    Leiden Institute for Brain and Cognition, Leiden University Medical Center, Leiden, The Netherlands
    J Cereb Blood Flow Metab 31:1321-33. 2011
    ..Corroborating previous PET results, our findings suggest that PCASL is a promising tool for central nervous system drug research...
  59. pmc A fast analysis method for non-invasive imaging of blood flow in individual cerebral arteries using vessel-encoded arterial spin labelling angiography
    Michael A Chappell
    Institute of Biomedical Engineering, University of Oxford, Old Road Campus, Headington, Oxford OX3 7DQ, UK
    Med Image Anal 16:831-9. 2012
    ..The final algorithm is demonstrated on in vivo data with analysis on a time scale of the order of 10min, from both a healthy control and a patient with a vertebro-basilar occlusion...
  60. ncbi Pseudocontinuous arterial spin labeling perfusion magnetic resonance imaging--a normative study of reproducibility in the human brain
    Wen Chau Wu
    Graduate Institute of Oncology, National Taiwan University, Taipei, Taiwan
    Neuroimage 56:1244-50. 2011
    ..We concluded that CS correction is necessary for ASL imaging when using phased array receiver and that after CS correction, PCASL requires α correction to provide reproducibility comparable to PASL...
  61. ncbi A kinetic model for vessel-encoded dynamic angiography with arterial spin labeling
    Thomas W Okell
    Centre for Functional Magnetic Resonance Imaging of the Brain, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United Kingdom
    Magn Reson Med 68:969-79. 2012
    ..Relative volume flow rates in downstream vessels are also quantified, showing the relative importance of each feeding artery. This framework is likely to be of use in assessing collateral blood flow in patient groups...
  62. ncbi Three-spin correlations in double electron-electron resonance
    Gunnar Jeschke
    ETH Zurich, CH 8093 Zurich, Switzerland
    Phys Chem Chem Phys 11:6580-91. 2009
    ..pulse electron paramagnetic resonance techniques are increasingly applied to systems that contain more than two spin labels. Under typical experimental conditions this leads to signal contributions from sum and difference combinations ..
  63. pmc Measuring the dynamic surface accessibility of RNA with the small paramagnetic molecule TEMPOL
    Vincenzo Venditti
    Biomolecular Structure Research Center and Dipartimento di Biologia Molecolare, Universita di Siena, Via Fiorentina 1, 53100 Siena, Italy
    Nucleic Acids Res 36:e20. 2008
    ..We conclude that TEMPOL probing can provide valuable insights into the surface accessibility and dynamics of RNA, and can also be used as an independent means of validating RNA structure and dynamics in solution...
  64. pmc Effects of tempol and redox-cycling nitroxides in models of oxidative stress
    Christopher S Wilcox
    Division of Nephrology and Hypertension, Department of Medicine and Center for Hypertension, Kidney and Vascular Health, Georgetown University, Washington, DC, United States
    Pharmacol Ther 126:119-45. 2010
    ..Indeed, tempol given from birth prolonged the life span of normal mice. However, presently tempol has been used only in human subjects as a topical agent to prevent radiation-induced alopecia...
  65. pmc High-resolution distance mapping in rhodopsin reveals the pattern of helix movement due to activation
    Christian Altenbach
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 105:7439-44. 2008
    ..The positions of nitroxides in other helices at the cytoplasmic surface remained largely unchanged...
  66. ncbi Implementation of quantitative perfusion imaging using pulsed arterial spin labeling at ultra-high field
    A G Gardener
    Sir Peter Mansfield Magnetic Resonance Centre, School of Physics and Astronomy, University of Nottingham, United Kingdom
    Magn Reson Med 61:874-82. 2009
    ..An increase in PW signal was found with field strength, and together with the increased signal-to-noise ratio, this led to improved image signal-to-noise and quality of fit of perfusion maps at 7 T...
  67. ncbi Long-term administration of tempol attenuates postinfarct ventricular dysfunction and sympathetic activity in rats
    Zhen Shi
    Department of Physiology, Nanjing Medical University, Nanjing 210029, China
    Pflugers Arch 458:247-57. 2009
    ....
  68. ncbi Inhibition of in vivo leishmanicidal mechanisms by tempol: nitric oxide down-regulation and oxidant scavenging
    Edlaine Linares
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, CP 26077, CEP 05513 970 Sao Paulo, SP, Brazil
    Free Radic Biol Med 44:1668-76. 2008
    ..Thus, the development of therapeutic strategies based on nitroxides should take into account the potential risk of altering host resistance to parasite infection...
  69. ncbi Studying biomolecular complexes with pulsed electron-electron double resonance spectroscopy
    Gunnar W Reginsson
    Biomedical Sciences Research Complex, Centre of Magnetic Resonance, University of St Andrews, St Andrews KY16 9ST, UK
    Biochem Soc Trans 39:128-39. 2011
    ..In the present review, the methods of PELDOR are discussed and examples where PELDOR has been used to obtain structural information on biomolecules are summarized...
  70. ncbi Evaluation of spin labels for in-cell EPR by analysis of nitroxide reduction in cell extract of Xenopus laevis oocytes
    Mykhailo Azarkh
    University of Konstanz, Departments of Chemistry and Biology, Konstanz Research School Chemical Biology KoRS CB, Zukunftskolleg, 78457 Konstanz, Germany
    J Magn Reson 212:450-4. 2011
    ..these methods at physiological temperatures for in-cell studies is hampered by reduction of the nitroxide spin labels and thus short half-lives in the cellular environment...
  71. ncbi Cardiac oxidative stress in acute and chronic isoproterenol-infused rats
    Guo Xing Zhang
    Department of Pharmacology, Kagawa University Medical School, 1750 1 Ikenobe, Miki, Kagawa 761 0793, Japan
    Cardiovasc Res 65:230-8. 2005
    ..This study aimed to clarify the role of reactive oxygen species (ROS) in mitogen-activated protein (MAP) kinase activation and tissue remodeling of the heart of isoproterenol (ISO)-infused rats...
  72. pmc NADPH oxidase-derived superoxide anion mediates angiotensin II-enhanced carotid body chemoreceptor sensitivity in heart failure rabbits
    Yu Long Li
    Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE 68198 5850, USA
    Cardiovasc Res 75:546-54. 2007
    ..NADPH oxidase-derived superoxide anion mediates the effects of Ang II in many organs. We investigated whether this signaling pathway may mediate the enhanced peripheral chemoreceptor sensitivity induced by Ang II in CHF rabbits...
  73. ncbi Pulsed electron-electron double resonance: beyond nanometre distance measurements on biomacromolecules
    Gunnar W Reginsson
    Biomedical Sciences Research Complex, Centre of Magnetic Resonance, University of St Andrews, St Andrews, Scotland, UK
    Biochem J 434:353-63. 2011
    ..Small nitroxide spin labels, paramagnetic metal ions, amino acid radicals or intrinsic clusters and cofactor radicals have been used as ..
  74. ncbi Whole-brain 3D perfusion MRI at 3.0 T using CASL with a separate labeling coil
    S Lalith Talagala
    NINDS, National Institutes of Health, Bethesda, Maryland 20892 1060, USA
    Magn Reson Med 52:131-40. 2004
    ..The current method can be extended to higher fields, and further improved by the use of multiple receiver coils and parallel imaging techniques to reduce scan time or provide increased resolution...
  75. pmc Monomeric and dimeric conformation of the vinculin tail five-helix bundle in solution studied by EPR spectroscopy
    Christoph Abé
    Department of Physics, University of Osnabruck, Osnabruck, Germany
    Biophys J 101:1772-80. 2011
    ..Furthermore, the distance between positions 1033 is shown to increase by >3 nm upon interaction of the vinculin tail domain with F-actin...
  76. ncbi Identification of free radicals on hemoglobin from its self-peroxidation using mass spectrometry and immuno-spin trapping: observation of a histidinyl radical
    Leesa J Deterding
    Laboratory of Structural Biology, Laboratory of Pharmacology and Chemistry, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 279:11600-7. 2004
    ..Because of the pi-pi interaction of Tyr-24 and His-20, the unpaired electron is apparently delocalized on both the tyrosine and histidine residue (pi-pi stacked pair radical)...
  77. ncbi Low spin-lock field T1 relaxation in the rotating frame as a sensitive MR imaging marker for gene therapy treatment response in rat glioma
    Mikko I Kettunen
    Department of Biotechnology and Molecular Medicine, A I Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland
    Radiology 243:796-803. 2007
    ....
  78. ncbi Factors influencing nitroxide reduction and cytotoxicity in vitro
    Yuval Samuni
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Antioxid Redox Signal 6:587-95. 2004
    ....
  79. ncbi Determination of partition coefficient of spin probe between different lipid membrane phases
    Zoran Arsov
    Laboratory of Biophysics, Jozef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia
    J Chem Inf Model 45:1662-71. 2005
    ..The results indicate that the used spin probe partitions approximately equally between different phases...
  80. ncbi Multimodal functional cardiac MRI in creatine kinase-deficient mice reveals subtle abnormalities in myocardial perfusion and mechanics
    Matthias Nahrendorf
    Medizinische Klinik und Poliklinik 1, Universitat Wurzburg, Josef Schneider Strasse 2, 97080 Wurzburg, Germany
    Am J Physiol Heart Circ Physiol 290:H2516-21. 2006
    ..The myocardium in CK-deficient mice is characterized by reduced perfusion and reduced maximal contraction velocity, suggesting that the myocardial hypertrophy seen in these mice cannot fully compensate for the absence of the CK system...
  81. ncbi Mapping global folds of oligonucleotides by pulsed electron-electron double resonance
    Olav Schiemann
    Centre for Biomolecular Sciences, Centre of Magnetic Resonance, University of St Andrews, St Andrews, United Kingdom
    Methods Enzymol 469:329-51. 2009
    ..This chapter briefly summarizes the theory, describes how to perform and analyze such experiments and discusses the limitations...
  82. pmc Algorithm for selection of optimized EPR distance restraints for de novo protein structure determination
    Kelli Kazmier
    Chemical and Physical Biology Program, 340 Light Hall, Vanderbilt University, Nashville, TN 37232, USA
    J Struct Biol 173:549-57. 2011
    ..subdomain of T4 lysozyme (T4L), simulated restraints that maximize sequence separation between the two spin labels while simultaneously ensuring pairwise connectivity of secondary structure elements yielded vastly improved ..
  83. ncbi PELDOR study of conformations of double-spin-labeled single- and double-stranded DNA with non-nucleotide inserts
    Nikita A Kuznetsov
    Institute of Chemical Biology and Fundamental Medicine, Lavrentyev Ave 8, 630090, Novosibirsk, Russia
    Phys Chem Chem Phys 11:6826-32. 2009
    ..Two nitroxide spin labels 4-amino-2,2,6,6-tetramethylpiperidine-1-oxyl were attached at the two ends of the molecules...
  84. ncbi Amplitude of pancreatic lipase lid opening in solution and identification of spin label conformational subensembles by combining continuous wave and pulsed EPR spectroscopy and molecular dynamics
    Sebastien Ranaldi
    CNRS Laboratoire de Bioenergetique et Ingenierie des Proteines, UPR 9036, Institut de Microbiologie de la Mediterranee, Aix Marseille Universités, Marseille, France
    Biochemistry 49:2140-9. 2010
    ..to the active site of human pancreatic lipase (HPL) was measured from the magnetic interaction between two spin labels grafted on this enzyme...
  85. ncbi Two-compartment exchange model for perfusion quantification using arterial spin tagging
    J Zhou
    Department of Radiology, Johns Hopkins University Medical School, Baltimore, Maryland 21205-2195, USA
    J Cereb Blood Flow Metab 21:440-55. 2001
    ..FAIR flow values with correction for restricted exchange, however, correlate well with the radioactive microsphere flow values. The limitations of the approach in terms of choice of the intercompartmental exchange rates are discussed...
  86. ncbi The effect of lipophilicity of spin-labeled compounds on their distribution in solid lipid nanoparticle dispersions studied by electron paramagnetic resonance
    Ahlin Pegi
    University of Ljubljana, Faculty of Pharmacy, Askerceva 7, 1000 Ljubljana, Slovenia
    J Pharm Sci 92:58-66. 2003
    ....
  87. pmc A structure-based simulation approach for electron paramagnetic resonance spectra using molecular and stochastic dynamics simulations
    Christian Beier
    Fachbereich Physik, Universitat Osnabruck, Osnabruck, Germany
    Biophys J 91:2647-64. 2006
    ..Furthermore, effects on the motional freedom of bound spin-labels induced by solubilization of bacteriorhodopsin with Triton X-100 are examined...
  88. ncbi Superselective pseudocontinuous arterial spin labeling
    Michael Helle
    Institute of Neuroradiology, Christian Albrechts Universitat, Kiel, Germany
    Magn Reson Med 64:777-86. 2010
    ..This shows the method's flexibility for dealing with complicated arterial geometries and its ability to superselectively label small intracranial arteries...
  89. pmc Studying RNA using site-directed spin-labeling and continuous-wave electron paramagnetic resonance spectroscopy
    Xiaojun Zhang
    Department of Chemistry, University of Southern California, Los Angeles, California, USA
    Methods Enzymol 469:303-28. 2009
    ..Various approaches for attaching nitroxide spin labels to nucleic acids are described, followed by detailed descriptions of cw-EPR spectral acquisition and processing ..
  90. pmc Structural origin of weakly ordered nitroxide motion in spin-labeled proteins
    Mark R Fleissner
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095 7008
    Protein Sci 18:893-908. 2009
    ....
  91. ncbi High-field EPR
    Anton Savitsky
    Department of Physics, Free University Berlin, Arnimallee 14, 14195 Berlin, Germany
    Photosynth Res 102:311-33. 2009
    ..The information obtained is complementary to that of protein crystallography, solid-state NMR and laser spectroscopy...
  92. ncbi Site-directed spin-labeling of nucleic acids by click chemistry: detection of abasic sites in duplex DNA by EPR spectroscopy
    Ulla Jakobsen
    Department of Physics and Chemistry, University of Southern Denmark, Nucleic Acid Center Campusvej 55, 5230 Odense, Denmark
    J Am Chem Soc 132:10424-8. 2010
    ..The ease of incorporation and properties of the new spin label make it attractive for EPR studies of nucleic acids and other macromolecules...
  93. ncbi Vessel-encoded arterial spin-labeling using pseudocontinuous tagging
    Eric C Wong
    Department of Radiology, University of California, San Diego, La Jolla, CA, USA
    Magn Reson Med 58:1086-91. 2007
    ..High SNR maps of left carotid, right carotid, and basilar territories are generated in 6 min of scan time...
  94. ncbi Nitric oxide- and superoxide-dependent mitochondrial signaling in endotoxin-induced apoptosis in the rostral ventrolateral medulla of rats
    Samuel H H Chan
    Center for Neuroscience, National Sun Yat Sen University, Kaohsiung 804, Taiwan, Republic of China
    Free Radic Biol Med 39:603-18. 2005
    ..We conclude that caspase-dependent apoptotic cell death that results from NO- and O(2)(-)-associated mitochondrial signaling in the RVLM may underlie fatal cardiovascular depression during endotoxemia...
  95. pmc Elucidating transient macromolecular interactions using paramagnetic relaxation enhancement
    G Marius Clore
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0520, USA
    Curr Opin Struct Biol 17:603-16. 2007
    ....
  96. pmc The role of reactive oxygen species in capsaicin-induced mechanical hyperalgesia and in the activities of dorsal horn neurons
    Inhyung Lee
    Department of Neuroscience and Cell Biology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555 1069, USA
    Pain 133:9-17. 2007
    ..Removal of ROS thus restored the responsiveness of spinal WDR neurons to normal levels, suggesting that ROS is involved in central sensitization, at least in part by sensitizing WDR neurons...
  97. ncbi The effect of tempol on endothelium-dependent vasodilatation and blood pressure
    Ulf Simonsen
    Department of Pharmacology, Faculty of Health Sciences, University Park 240, University of Aarhus, 8000 Aarhus C, Denmark
    Pharmacol Ther 122:109-24. 2009
    ..In summary, lowering of the oxidative stress by scavenging of free radicals may offer a new antihypertensive treatment strategy...
  98. pmc Tempol-mediated activation of latent iron regulatory protein activity prevents symptoms of neurodegenerative disease in IRP2 knockout mice
    Manik C Ghosh
    National Institute of Child Health and Human Development and Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 105:12028-33. 2008
    ....
  99. pmc Structural determinants of nitroxide motion in spin-labeled proteins: tertiary contact and solvent-inaccessible sites in helix G of T4 lysozyme
    Zhefeng Guo
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095 7008, USA
    Protein Sci 16:1069-86. 2007
    ..Residue 118 is at a solvent-inaccessible site in the protein core, and the structure of 118R1, the first reported for the R1 side chain at a buried site, reveals how the side chain is accommodated in an overpacked core...
  100. pmc Multifrequency electron spin resonance spectra of a spin-labeled protein calculated from molecular dynamics simulations
    Deniz Sezer
    Department of Physics, Cornell University, Ithaca, New York 14853, USA
    J Am Chem Soc 131:2597-605. 2009
    ..During the trajectories, the spin labels interconvert among a fairly large number of conformations and display a propensity to form interactions with ..
  101. pmc Clinical neuroimaging using arterial spin-labeled perfusion magnetic resonance imaging
    Ronald L Wolf
    Department of Radiology, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104, USA
    Neurotherapeutics 4:346-59. 2007
    ..Recent technical advances have improved the sensitivity of ASL perfusion MRI, and increasing use is expected in the coming years. The present review focuses on ASL perfusion MRI and applications in clinical neuroimaging...

Research Grants64

  1. Structural Dynamics of Cardiac Muscle Contraction
    Brett A Colson; Fiscal Year: 2012
    ..There are three specific aims: (1) Develop spectroscopic methods, involving EPR of spin labels specifically bound to myosin or actin, to measure accurately the effects of genetic ablation of cMyBP-C on ..
  2. Structure of the GABA-A Receptor Binding Sites
    CYNTHIA M CZAJKOWSKI; Fiscal Year: 2013
    ..motions in the prokaryotic pLGIC homologs from Gloeobacter violaceus (GLIC) using site-directed spin labels and electron paramagnetic resonance spectroscopy (SDSL- EPR) and to test these motions in the GABAAR using an ..
  3. Structure of the GABA-A Receptor Binding Sites
    CYNTHIA M CZAJKOWSKI; Fiscal Year: 2012
    ..motions in the prokaryotic pLGIC homologs from Gloeobacter violaceus (GLIC) using site-directed spin labels and electron paramagnetic resonance spectroscopy (SDSLEPR) and to test these motions in the GABAAR using an ..
  4. STRUCTURAL ANALYSIS OF THE MANNOSE 6-PHOSPHATE RECEPTORS
    Nancy M Dahms; Fiscal Year: 2013
    ..These studies will be complemented by EPR spectroscopic analyses of MPRs containing site-specific spin labels (Aim 4)...
  5. Structural Dynamics of Multi-drug Transporters
    Hassane S McHaourab; Fiscal Year: 2013
    ..and Q-band pulse EPR, to increase the throughput of long range distance measurements (up to 70E) between spin labels by double electron electron resonance (DEER)...
  6. Structural Dynamics of Cardiac Calcium Transport Regulation
    NAA ADJELEY DROMOH ABLORH; Fiscal Year: 2013
    ..The approach is to attach paramagnetic probes (nitroxide spin labels) to selected sites on synthetic variants of PLB, reconstitute spin-labeled PLB into lipid bilayers in the ..
  7. PROTEIN FOLDING &UNFOLDING BY DQC CYTOCHROME C
    Jack H Freed; Fiscal Year: 2011
    ..The GdmCl concentrations used were 0.7, 1.5, and 2.8M. In the folded state, the distance between spin labels was 15.8/0.5 [unreadable]. At [GdmCl] of 0...
  8. Reactive Intermediates in Lipoxygenase Pathways
    Betty J Gaffney; Fiscal Year: 2012
    ..Positions of introduced spin labels within the structure will be determined by triangulation and modeling, to facilitate subsequent protein-ligand ..
  9. Development of Biomedical EPR Instrumentation
    James S Hyde; Fiscal Year: 2013
    ..methods for SR followed by measurements across the experimental parameters of the phase diagram using available spin labels. (2) Use of the data of Specific Aim 1 to design analogous ELDOR experiments, which will also be applied across ..
  10. Pulsed Q-band EPR Spectrometer
    Albert H Beth; Fiscal Year: 2010
    ..analysis of DEER data;4) provide orientation selectivity for assessment of relative orientations between spin labels;and 5) provide the capability to make reliable cw-Q-band measurements...
  11. METAL SITES AND DYNAMICS IN LARGE RNA MOLECULES
    Victoria J DeRose; Fiscal Year: 2010
    ..Insight into RNA conformational changes will be sought using site-specific, EPR-active nitroxide spin labels. Taken together, these studies will provide important information about RNA structure as a function of solution ..
  12. IMMUNOLOGICAL STUDIES OF VISUAL TRANSDUCTION PATHWAYS
    Heidi E Hamm; Fiscal Year: 2013
    ....
  13. Time-dependent structural studies on dinuclear metal ion containing enzymes
    David L Tierney; Fiscal Year: 2010
    ..turnover, through CW EPR studies of Co-containing derivatives of L1 incorporating a series of site-directed spin labels (SDSL);these studies will be extended into the time domain using RFQ-EPR to probe larger scale motions of the ..
  14. National Biomedical EPR Center
    James S Hyde; Fiscal Year: 2013
    ..usage in NIH-funded grants is in the field of site-directed spin-labeling (SDSL) using nitroxide radical spin labels to probe molecular structure...
  15. Cantilever Magnetic Resonance of Biomolecules
    John A Marohn; Fiscal Year: 2012
    ..create a "molecular microscope" capable of imaging the proton density of, or mapping the locations of electron spin labels in, a single copy of a protein or macromolecular complex...
  16. ELECTRON SPIN RELAXATION IN MODEL MEMBRANES
    Jack Freed; Fiscal Year: 2002
    ..abstract_text> ..
  17. DEVELOPMENT OF BIOMEDICAL ESR INSTRUMENTATION
    James Hyde; Fiscal Year: 2002
    ..proposal is to develop pulse saturation-recovery (SR) electron paramagnetic resonance (EPR) for application to spin labels in a context of site directed spin labeling (SDSL)...
  18. ANALYSIS OF MOTOR PROTEIN FUNCTION
    Edward Pate; Fiscal Year: 2004
    ..The corresponding regions in kinesin-family motors will also be probed with spin labels. Orientation information from EPR probes will be obtained using protocols I have developed to flow-align ..
  19. Protein Structure by EPR Distance Determination and Molecular Modeling
    ERIC HUSTEDT; Fiscal Year: 2007
    ..MC) modeling strategies will be developed to predict distance distributions between pairs of dipolar coupled spin labels measured by either CW-EPR or double electron-electron resonance (DEER) spectroscopy...
  20. MOLECULAR AND CELLULAR MECHANICS OF MUSCLE
    VINCENT BARNETT; Fiscal Year: 2001
    ..We will specifically modify myosin in situ using site-directed probes (spin labels, fluorescent probes and/or cross-linkers)...
  21. INTERACTION OF SPIN LABELS WITH TRANSITION METALS
    GARETH EATON; Fiscal Year: 2002
    ..The sites to be studied by EPR can be naturally-occurring metal ions or radicals, or spin labels or metals attached at selected locations introduced by site-directed mutagenesis...
  22. Substrate & Inhibitor Binding in Leucine Aminopeptidase
    Brian Bennett; Fiscal Year: 2007
    ..Structural information will be obtained from analysis of dipolar couplings between spin labels and paramagnetic transition ions in the active site...
  23. Studying the dynamics of protein allostery with path-based Markov state models
    ALBERT PAN; Fiscal Year: 2009
    ..serve initially to guide the experiments, for example, providing suggestions about relevant positions to attach spin labels. The experiments will in turn serve as an important validation of the computational methods...
  24. IMAGING OXYGEN--LOW FREQUENCY ELECTRON SPIN RESONANCE
    Howard Halpern; Fiscal Year: 1993
    ..the sensitivity of the electron spin resonance (ESR) spectra of certain injectable compounds, nitroxide spin labels or spin probes, to molecular oxygen concentration...
  25. Structure and dynamics of a homologue of neurotransmitter sodium symporters
    DEREK CLAXTON; Fiscal Year: 2009
    ..b>Spin labels will be systematically introduced into the protein sequence and analyzed in both a detergent and lipid ..
  26. MOLECULAR DYNAMICS OF MUSCLE CONTRACTION
    David Thomas; Fiscal Year: 2003
    ..Site-selective molecular probes (spin labels or luminescent dyes) will be attached to myosin or actin, and spectroscopy experiments (electron paramagnetic ..
  27. ESR SPIN LABEL STUDIES OF FIBRONECTIN
    Ching San Lai; Fiscal Year: 1993
    ..This permits the placement of spin labels or fluorescent labels into the recombinant fibronectin fragment and the elucidation of detailed structural ..
  28. Structure and Function of the Mitochondrial Uncoupling Proteins
    James Chou; Fiscal Year: 2007
    ..is the use of orientation restraints in the form of dipolar couplings and distance restraints from paramagnetic spin labels to obtain high-resolution structure with minimal amount of NMR spectral analysis...
  29. TOPICAL IN-VIVO ELECTRON SPIN RESONANCE
    Lawrence Berliner; Fiscal Year: 1993
    ..properties which distinguish it from other techniques: a) In-vivo detection of extrinsic paramagnetic species (spin labels) targeted to specific sites in a biological system...
  30. STRUCTURE AND DYNAMICS OF RIBOZYMES BY EPR
    SNORRI SIGURDSSON; Fiscal Year: 2002
    ..This incorporation of stable free radicals (spin labels) into the nucleic acids and data analysis of their EPR spectra...
  31. FOLDING OF CARBONIC ANHYDRASE
    Sandra Eaton; Fiscal Year: 1999
    ..Both techniques will be tested with spin labels at known locations in normally folded carbonic anhydrase...
  32. PASADENA: Ultra-fast Carbon 13 Imaging for Early Diagnosis of Cancer
    Pratip Bhattacharya; Fiscal Year: 2007
    ..Clinical Trials of PASADENA molecular brain imaging: R21 Specific Aims: (1) Develop novel carbon (and nitrogen) spin labels as in vivo PASADENA imaging reagents...
  33. PHOSPHATE TRANSPORT PATHS WITHIN HOMODIMERIC PTP
    Hartmut Wohlrab; Fiscal Year: 2001
    ..the help of the photoaffinity label that the Pi-accessible sites are on different subunits and with the help of spin labels that the two sites on the same side of the membrane have different conformations...
  34. INTERACTION OF ATP ANALOGUES WITH MYOSIN
    RALPH YOUNT; Fiscal Year: 2002
    ..These groups will include spin labels, fluorescent and luminescent probes...
  35. NATIONAL BIOMEDICAL ESR CENTER
    James Hyde; Fiscal Year: 2001
    ..world with balanced separately funded programs of health related research in the major branches of the field: spin labels, transition metals, and free radicals...
  36. INTERACTION OF ATP ANALOGUES WITH MYOSIN
    RALPH YOUNT; Fiscal Year: 2001
    ..These groups will include spin labels, fluorescent and luminescent probes...
  37. AGE AND MUSCLE STRENGTH--ROLE OF MYOSIN
    LaDora Thompson; Fiscal Year: 2000
    ..b>Spin labels will be attached to a selected site on the myosin head within muscle fibers and then EPR will be used to detect ..
  38. SOLVENT AND SMALL SOLUTES IN TISSUES AND PROTEINS
    Robert Bryant; Fiscal Year: 1980
    ..a dry protein system will be investigated by study of the protein NMR spectra and relaxation of rare spins or spin labels in the solid state...
  39. PROTEIN STUDIES WITH UNNATURAL AMINO ACIDS
    Peter Schultz; Fiscal Year: 2000
    ..In addition, analogues with altogether new properties can be introduced including spin labels, photoactivable and redox active amino acids...
  40. SPECTROSCOPIC APPROACHES TO GENE REGULATION
    Ponzy Lu; Fiscal Year: 1990
    ..Using operator or promoter DNA sequences with specific nuclear spin labels we will study the changes in the DNA structure and the nature of the contracts that lead to the specificity of ..
  41. IN VIVO EPR SPECTROMETRY VIA 200 MHZ FIELD SWITCHING
    Joseph Ackerman; Fiscal Year: 1992
    ..This will allow a wide range of electron spin labels to be used...
  42. MULTIUSER EPR SPECTROMETER AT UCSD
    Mark Thiemens; Fiscal Year: 1999
    ..The total cost of the system is 390,571 dollars. An Administrative Arrangement and a Long-Term Operation Plan are presented. ..
  43. FOLDING AND STABILITY OF PROTEIN DOMAINS AND SUBDOMAINS
    Daniel Raleigh; Fiscal Year: 2000
    ..These experiments will make use of stable spin labels and 2D NMR...
  44. MACROMOLECULAR HYDRATION AND STRUCTURE
    Irwin Kuntz; Fiscal Year: 1990
    ..We also suggest studies of protein folding based on the use of spin labels to learn about the structure of kinetic intermediates and the use of specific amino acid substitutions to study ..
  45. SPIN-LABELLED PURINE NUCLEOSIDES AND NUCLEOTIDES
    John Hunt; Fiscal Year: 1980
    ..The spin-labeled compounds will be tested for activity with enzymes and cells at different specificities, and will be used as spin-labels in studies of the active site of glutamine synthetase...
  46. Spectroscopic Probes of Smooth Muscle Functional Dynamics
    Joseph M Muretta; Fiscal Year: 2010
    ..Our molecular measurements in contracting muscle can provide the basis for evaluation of drugs and other therapeutic approaches. ..
  47. Site Directed Spin Labeling to Probe Acto-myosin Binding
    Vicci Korman; Fiscal Year: 2003
    ..specific actin residues, which are predicted to form the actin-myosin interface, into cysteine and attach spin labels selectively to these residues...
  48. Development of Arterial Spin Labeling for Clinical Applications
    Eric Wong; Fiscal Year: 2009
    ..The result of this work will be robust methods for measuring not only the amount of perfusion, but also the vascular source from which that perfusion was derived. ..
  49. Arterial Spin Labeling for fMRI
    Eric Wong; Fiscal Year: 2002
    ....
  50. Structure, dynamics, and function of the packaging RNA
    Peter Z Qin; Fiscal Year: 2010
    ..Understanding phi29 packaging motor function will aid in developing pharmacological approaches to combat these viruses. ..
  51. INTERACTION OF SPIN LABELS WITH TRANSITION METALS
    GARETH EATON; Fiscal Year: 2008
    ..abstract_text> ..
  52. Fast, Quantitative, Perfusion-Based functional-MRI
    Luis Hernandez Garcia; Fiscal Year: 2007
    ..These developments will be quite instrumental to the use of ASL perfusion imaging as a powerful tool for the study of brain function. ..
  53. PHYSIOLOGY & PATHOPHYSIOLOGY OF ACTIVATION FLOW COUPLING
    John Detre; Fiscal Year: 2002
    ..These studies will provide further insight into the parameters affecting the AFC response, and will suggest approaches for optimizing and interpreting functional imaging data. ..
  54. Perfusion fMRI in Cocaine Addiction
    John Detre; Fiscal Year: 2004
    ..abstract_text> ..
  55. MRI OF STRUCTURE/FUNCTION IN TEMPORAL LOBE EPILEPSY
    John Detre; Fiscal Year: 2003
    ..Validation of an fMRI protocol for assessing memory function will improve the safety and quality of the preoperative assessment for temporal lobectomy, and may have important implications for neurosurgical planning and prognosis. ..
  56. Perfusion MRI for multisite studies of brain function
    John A Detre; Fiscal Year: 2010
    ..The resulting technology will be made available to the biomedical community as a research product. ..
  57. Assessing Microstructure of Carotid Disease with MRI/MRS
    Ronald Wolf; Fiscal Year: 2006
    ..Plaque lipid profile and calcium content will be studied as a means to characterize the lesion and evaluate the association between plaque characteristics and patients' symptomatology. ..
  58. SVM based group data analysis for drug abuse disorder ASL perfusion study
    Ze Wang; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  59. Lung Perfusion Heterogeneity and Mechanisms of Edema
    Susan R Hopkins; Fiscal Year: 2010
    ..abstract_text> ..
  60. Pediatric template of brain perfusion
    Jiongjiong Wang; Fiscal Year: 2006
    ....
  61. REGULATION OF RENAL FUNCTION AND BP BY THROMBOXANE
    CHRISTOPHER WILCOX; Fiscal Year: 2002
    ....
  62. The Hemodynamic Response to Brain Activation
    Richard Buxton; Fiscal Year: 2005
    ..abstract_text> ..
  63. NMR Measurement of Perfusion in Skeletal Muscle & Brain
    Kenneth Marro; Fiscal Year: 2004
    ..Our intention is to expand this work into significant clinical and basic science programs in future grants. ..