monovalent cations

Summary

Summary: Positively charged atoms, radicals or group of atoms with a valence of plus 1, which travel to the cathode or negative pole during electrolysis.

Top Publications

  1. ncbi Monovalent cation permeability and Ca(2+) block of the store-operated Ca(2+) current I(CRAC )in rat basophilic leukemia cells
    Daniel Bakowski
    Laboratory of Cellular and Molecular Signalling, Department of Physiology, University of Oxford, Parks Road, Oxford OX1 3PT, UK
    Pflugers Arch 443:892-902. 2002
  2. pmc Activation and inhibition of histone deacetylase 8 by monovalent cations
    Stephanie L Gantt
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Biol Chem 285:6036-43. 2010
  3. pmc Acinetobacter baumannii increases tolerance to antibiotics in response to monovalent cations
    M Indriati Hood
    Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN 37232 2363, USA
    Antimicrob Agents Chemother 54:1029-41. 2010
  4. ncbi DNA G-quartets in a 1.86 A resolution structure of an Oxytricha nova telomeric protein-DNA complex
    M P Horvath
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309 0215, USA
    J Mol Biol 310:367-77. 2001
  5. ncbi Locating monovalent cations in the grooves of B-DNA
    S B Howerton
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332-0400, USA
    Biochemistry 40:10023-31. 2001
  6. pmc AtHKT1 is a salt tolerance determinant that controls Na(+) entry into plant roots
    A Rus
    Center for Plant Environmental Stress Physiology, Purdue University, West Lafayette, IN 47907 1165, USA
    Proc Natl Acad Sci U S A 98:14150-5. 2001
  7. pmc Separation and characterization of currents through store-operated CRAC channels and Mg2+-inhibited cation (MIC) channels
    Murali Prakriya
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Gen Physiol 119:487-507. 2002
  8. ncbi Cell age-related monovalent cations content and density changes in stored human erythrocytes
    G Minetti
    Dipartimento di Biochimica, Sezione di Scienze, Universita di Pavia, Italy
    Biochim Biophys Acta 1527:149-55. 2001
  9. ncbi Activation of ribokinase by monovalent cations
    C Evalena Andersson
    Department of Cell and Molecular Biology, Uppsala University, S 751 24 Uppsala, Sweden
    J Mol Biol 315:409-19. 2002
  10. ncbi Structural replacement of active site monovalent cations by the epsilon-amino group of lysine in the ATPase fragment of bovine Hsc70
    S M Wilbanks
    Beckman Laboratories for Structural Biology, Department of Structural Biology, Stanford University School of Medicine, California 94305 5400, USA
    Biochemistry 37:7456-62. 1998

Detail Information

Publications215 found, 100 shown here

  1. ncbi Monovalent cation permeability and Ca(2+) block of the store-operated Ca(2+) current I(CRAC )in rat basophilic leukemia cells
    Daniel Bakowski
    Laboratory of Cellular and Molecular Signalling, Department of Physiology, University of Oxford, Parks Road, Oxford OX1 3PT, UK
    Pflugers Arch 443:892-902. 2002
    Like voltage-operated Ca(2+) channels, store-operated CRAC channels become permeable to monovalent cations in the absence of external divalent cations...
  2. pmc Activation and inhibition of histone deacetylase 8 by monovalent cations
    Stephanie L Gantt
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Biol Chem 285:6036-43. 2010
    ..Two bound monovalent cations (MVCs) of unknown function have been previously observed in crystal structures of HDAC8; site 1 is near the ..
  3. pmc Acinetobacter baumannii increases tolerance to antibiotics in response to monovalent cations
    M Indriati Hood
    Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN 37232 2363, USA
    Antimicrob Agents Chemother 54:1029-41. 2010
    ..Taken together, these data demonstrate that A. baumannii sets in motion a global regulatory cascade in response to physiological NaCl concentrations, resulting in broad-spectrum tolerance to antibiotics...
  4. ncbi DNA G-quartets in a 1.86 A resolution structure of an Oxytricha nova telomeric protein-DNA complex
    M P Horvath
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309 0215, USA
    J Mol Biol 310:367-77. 2001
    ....
  5. ncbi Locating monovalent cations in the grooves of B-DNA
    S B Howerton
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332-0400, USA
    Biochemistry 40:10023-31. 2001
    Here we demonstrate that monovalent cations can localize around B-DNA in geometrically regular, sequence-specific sites in oligonucleotide crystals...
  6. pmc AtHKT1 is a salt tolerance determinant that controls Na(+) entry into plant roots
    A Rus
    Center for Plant Environmental Stress Physiology, Purdue University, West Lafayette, IN 47907 1165, USA
    Proc Natl Acad Sci U S A 98:14150-5. 2001
    ..The hkt1 mutations have revealed the existence of another Na(+) influx system(s) whose activity is reduced by high [Ca(2+)](ext)...
  7. pmc Separation and characterization of currents through store-operated CRAC channels and Mg2+-inhibited cation (MIC) channels
    Murali Prakriya
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Gen Physiol 119:487-507. 2002
    ..physiological ionic conditions, removal of extracellular divalent cations makes them freely permeable to monovalent cations. Several past studies have concluded that under these conditions CRAC channels conduct Na(+) and Cs(+) with ..
  8. ncbi Cell age-related monovalent cations content and density changes in stored human erythrocytes
    G Minetti
    Dipartimento di Biochimica, Sezione di Scienze, Universita di Pavia, Italy
    Biochim Biophys Acta 1527:149-55. 2001
    ..This may stem from a different impact of storage on the imbalance of monovalent cations, Na(+) and K(+), in young and old erythrocytes, related to their different complement of cation transporters.
  9. ncbi Activation of ribokinase by monovalent cations
    C Evalena Andersson
    Department of Cell and Molecular Biology, Uppsala University, S 751 24 Uppsala, Sweden
    J Mol Biol 315:409-19. 2002
    ..The mechanism is probably general to ribokinases, to some adenosine kinases, and to other members of the larger family. A careful re-evaluation of the biochemical and structural data is suggested for other enzyme systems...
  10. ncbi Structural replacement of active site monovalent cations by the epsilon-amino group of lysine in the ATPase fragment of bovine Hsc70
    S M Wilbanks
    Beckman Laboratories for Structural Biology, Department of Structural Biology, Stanford University School of Medicine, California 94305 5400, USA
    Biochemistry 37:7456-62. 1998
    ..It is possible to construct a very good structural mimic of bound cation which suffices for substrate binding but not for catalytic activity...
  11. pmc The hammerhead cleavage reaction in monovalent cations
    E A Curtis
    Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge 02142, USA
    RNA 7:546-52. 2001
    ..These results suggest that a metal ion does not act as a base in the reaction, and that the effects of different metal ions on hammerhead cleavage rates primarily reflect structural contributions to catalysis...
  12. pmc Dissociation of the store-operated calcium current I(CRAC) and the Mg-nucleotide-regulated metal ion current MagNuM
    Meredith C Hermosura
    Laboratory of Cell and Molecular Signaling, Center for Biomedical Research at The Queen s Medical Center and John A Burns School of Medicine at the University of Hawaii, Honolulu, HI 96813, USA
    J Physiol 539:445-58. 2002
    ....
  13. ncbi Investigation of allosteric linkages in the regulation of tryptophan synthase: the roles of salt bridges and monovalent cations probed by site-directed mutation, optical spectroscopy, and kinetics
    E Weber-Ban
    Department of Biochemistry, University of California at Riverside, Riverside, California 92521, USA
    Biochemistry 40:3497-511. 2001
    ..The mechanistic implications of these findings both for substrate channeling and for catalysis are discussed...
  14. ncbi Permeation of monovalent cations through the non-capacitative arachidonate-regulated Ca2+ channels in HEK293 cells. Comparison with endogenous store-operated channels
    O Mignen
    Department of Pharmacology, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 276:21365-74. 2001
    ..However, the clear differences between the properties of these currents through ARC and SOC channels in the same cell confirm that these represent distinct conductances...
  15. pmc Distinct properties of CRAC and MIC channels in RBL cells
    J Ashot Kozak
    Department of Physiology and Biophysics, University of California at Irvine, Irvine, CA 92697, USA
    J Gen Physiol 120:221-35. 2002
    ..Inwardly rectifying CRAC channels admit monovalent cations when external divalent ions are removed...
  16. ncbi Cd2+ effects on respiration and swelling of rat liver mitochondria were modified by monovalent cations
    S M Korotkov
    Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Torez pr, Russian Federation
    J Inorg Biochem 70:17-23. 1998
    ....
  17. ncbi A preliminary investigation on the growth requirement for monovalent cations, divalent cations and medium ionic strength of marine actinomycete Salinispora
    Ginger Tsueng
    Nereus Pharmaceuticals, Inc, 10480 Wateridge Circle, San Diego, CA, 92121, USA
    Appl Microbiol Biotechnol 86:1525-34. 2010
    ..29 to 15.2 mS/cm). S. arenicola has a lower growth requirement for ionic strength than S. tropica and S. pacifica...
  18. ncbi Predicting stability of DNA duplexes in solutions containing magnesium and monovalent cations
    Richard Owczarzy
    Integrated DNA Technologies, 1710 Commercial Park, Coralville, Iowa 52241, USA
    Biochemistry 47:5336-53. 2008
    ..temperatures, transition enthalpies, entropies, and free energies in buffers containing magnesium and monovalent cations. The new correction function significantly improves the accuracy of predictions and accounts for ion ..
  19. ncbi Regulation of skeletal muscle Ca2+ release channel (ryanodine receptor) by Ca2+ and monovalent cations and anions
    G Meissner
    Department of Biochemistry, University of North Carolina, Chapel Hill, North Carolina 27599 7260, USA
    J Biol Chem 272:1628-38. 1997
    ..measurements, bell-shaped Ca2+ activation/inactivation curves were obtained in media containing different monovalent cations (Li+, Na+, K+, Cs+, and choline+) and anions (Cl-, Mes-, and Pipes-)...
  20. pmc Regulation of connexin hemichannels by monovalent cations
    Miduturu Srinivas
    State University of New York College of Optometry, New York, NY 10036, USA
    J Gen Physiol 127:67-75. 2006
    ..connexin, exhibit a novel form of regulation characterized by extraordinary sensitivity to extracellular monovalent cations. Replacement of extracellular Na+ with K+, while maintaining extracellular Ca2+ constant, resulted in >10-..
  21. ncbi pH and monovalent cations regulate cytosolic free Ca(2+) in E. coli
    Riffat Naseem
    Department of Medical Biochemistry and Immunology, Tenovus Building, Cardiff University, Heath Park, Cardiff, UK
    Biochim Biophys Acta 1778:1415-22. 2008
    The results here show for the first time that pH and monovalent cations can regulate cytosolic free Ca(2+) in E. coli through Ca(2+) influx and efflux, monitored using aequorin. At pH 7.5 the resting cytosolic free Ca(2+) was 0.2-0...
  22. ncbi Principles of RNA compaction: insights from the equilibrium folding pathway of the P4-P6 RNA domain in monovalent cations
    Keiji Takamoto
    Department of Physiology and Biophysics, Albert Einstein College of Medicine of Yeshiva University, New York, NY 10461, USA
    J Mol Biol 343:1195-206. 2004
    ..The folding model derived from these and previous results provides a robust framework for understanding the equilibrium and kinetic folding of RNA...
  23. ncbi A structural perspective on enzymes activated by monovalent cations
    Enrico Di Cera
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 281:1305-8. 2006
    Enzymes activated by monovalent cations are abundantly represented in plants and the animal world...
  24. pmc Extracellular divalent cations block a cation non-selective conductance unrelated to calcium channels in rat cardiac muscle
    K Mubagwa
    Centrum voor Experimentele Heelkunde en Anesthesiologie, University of Leuven, Belgium
    J Physiol 502:235-47. 1997
    ..6. These results suggest that a novel conductance pathway, permeable to monovalent cations but not to Cl- and blocked by divalent cations, exists in ventricular myocytes.
  25. ncbi 205Tl NMR methods for the characterization of monovalent cation binding to nucleic acids
    Michelle L Gill
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    J Am Chem Soc 127:16723-32. 2005
    b>Monovalent cations play an important role in many biological functions. The guanine rich sequence, d(G4T4G4), requires monovalent cations for formation of the G-quadruplex, d(G4T4G4)2...
  26. pmc Influence of extracellular monovalent cations on pore and gating properties of P2X7 receptor-operated single-channel currents
    T Riedel
    Julius Bernstein Institute for Physiology, Martin Luther University Halle, Halle Saale, Germany
    Biophys J 93:846-58. 2007
    Using the patch-clamp method, we studied the influence of external alkali and organic monovalent cations on the single-channel properties of the adenosine triphosphate (ATP)-activated recombinant human P2X(7) receptor...
  27. ncbi Activation of the Escherichia coli cell division protein FtsZ by a low-affinity interaction with monovalent cations
    Michael Tadros
    Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Madrid, Spain
    FEBS Lett 580:4941-6. 2006
    We have investigated the activation of FtsZ by monovalent cations. FtsZ polymerization was dependent on the concentrations of protein and monovalent salts, and was accompanied by the uptake of a single ion per monomer added...
  28. pmc Conduction properties of the cloned Shaker K+ channel
    L Heginbotham
    Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115
    Biophys J 65:2089-96. 1993
    ..6 M K+. The Shaker K+ channel is highly selective among monovalent cations; under bi-ionic conditions, its selectivity sequence is K+ > Rb+ > NH+4 > Cs+ > Na+, whereas, by ..
  29. ncbi HDV ribozyme activity in monovalent cations
    Anne T Perrotta
    Department of Biochemistry, Box 3711, Duke University Medical Center, Durham, North Carolina 27710, USA
    Biochemistry 45:11357-65. 2006
    ..Both ribozymes self-cleaved in high concentrations of monovalent cations, and an active site cytosine was required for cleavage activity under those conditions...
  30. ncbi Effects of external cations and mutations in the pore region on C-type inactivation of Shaker potassium channels
    J Lopez-Barneo
    Howard Hughes Medical Institute, Department of Molecular and Cellular Physiology, Beckman Center, Stanford University School of Medicine, California 94305
    Receptors Channels 1:61-71. 1993
    ..outer mouth of the pore) produce drastic changes in C-type inactivation kinetics and in its interaction with monovalent cations. Replacement of threonine in the wild-type by glutamic acid or lysine leads to a hundred-fold acceleration ..
  31. ncbi Structural roles of monovalent cations in the HDV ribozyme
    Ailong Ke
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720, USA
    Structure 15:281-7. 2007
    ..To investigate the role of monovalent cations in ribozyme structure and function, we determined the crystal structure of the precursor HDV ribozyme in the ..
  32. ncbi Monovalent cations affect the free solution mobility of DNA by perturbing the hydrogen-bonded structure of water
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
    Biopolymers 78:62-8. 2005
    ..electrophoresis in solutions of constant ionic strength containing a common anion and fifteen different monovalent cations. In solutions with the same ionic composition, the mobilities of different DNA molecules can vary by up to ..
  33. pmc Monovalent cations use multiple mechanisms to resolve ribozyme misfolding
    Yan Fei Jiang
    State Key Laboratory of Virology and Department of Biotechnology, College of Life Sciences, Wuhan University, Wuhan, Hubei 430072, PR China
    RNA 12:561-6. 2006
    Recent efforts have been made to unravel the independent roles of monovalent cations in RNA folding, primarily using the Tetrahymena ribozyme as a model. Here we report how monovalent cations impact the folding of the Candida ribozyme...
  34. ncbi Monovalent cations mediate formation of native tertiary structure of the Tetrahymena thermophila ribozyme
    Keiji Takamoto
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Nat Struct Biol 9:928-33. 2002
    ....
  35. ncbi Thrombin: a paradigm for enzymes allosterically activated by monovalent cations
    Enrico Di Cera
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    C R Biol 327:1065-76. 2004
    Enzymes activated by monovalent cations are abundantly represented in plants and in the animal world...
  36. ncbi Mechanosensitive ion channels in cultured sensory neurons of neonatal rats
    Hawon Cho
    The Sensory Research Center, Creative Research Initiatives, College of Pharmacy, Seoul National University, Seoul 151 742, Korea
    J Neurosci 22:1238-47. 2002
    ..Both LT and HT MS channels were permeable to monovalent cations and Ca2+ and were blocked by gadolinium, a blocker of MS channels...
  37. ncbi The hereditary stomatocytoses: genetic disorders of the red cell membrane permeability to monovalent cations
    Jean Delaunay
    Service d Hematologie, d Immunologie et de Cytogénétique, Hopital de Bicetre, Faculte de Medecine Paris Sud, INSERM U 473, 84 rue du General Leclerc, 94273 Le Kremlin Bicêtre, France
    Semin Hematol 41:165-72. 2004
    ..hereditary stomatocytoses are mostly accounted for by genetic disorders of red cell membrane permeability to monovalent cations. These conditions, all very rare, are comprised of a hemolytic anemia, frequently macrocytosis, and the ..
  38. ncbi Stabilization of RNA tertiary structure by monovalent cations
    R Shiman
    Department of Chemistry, Johns Hopkins University, 3400 N Charles Street, Baltimore, MD 21218, USA
    J Mol Biol 302:79-91. 2000
    The effects of monovalent cations (Li(+), Na(+), K(+), Rb(+), Cs(+), and NH4(+)) on the thermal stability of RNA tertiary structure were investigated by UV melting...
  39. ncbi The hammerhead, hairpin and VS ribozymes are catalytically proficient in monovalent cations alone
    J B Murray
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, USA
    Chem Biol 5:587-95. 1998
    ..The catalytic activity of RNA enzymes is thought to require divalent metal ions, which are believed to facilitate RNA folding and to play a direct chemical role in the reaction...
  40. ncbi Selective binding of monovalent cations to the stacking G-quartet structure formed by guanosine 5'-monophosphate: a solid-state NMR study
    Alan Wong
    Department of Chemistry, Queen s University, Kingston, Ontario, Canada K7L 3N6
    J Am Chem Soc 125:13895-905. 2003
    We report a solid-state multinuclear ((23)Na, (15)N, (13)C, and (31)P) NMR study on the relative affinity of monovalent cations for a stacking G-quartet structure formed by guanosine 5'-monophosphate (5'-GMP) self-association at pH 8...
  41. ncbi Apoptosis vs. oncosis: role of cell volume and intracellular monovalent cations
    Sergei N Orlov
    Centre de recherche du Centre hospitalier de l Université de Montréal CHUM Hôtel Dieu, 3850, rue Saint Urbain, Montreal, Quebec H2W 1T7, Canada
    Adv Exp Med Biol 559:219-33. 2004
    ..We examined this hypothesis as well as the role of monovalent cations as major intracellular osmolytes using vascular smooth muscle cells (VSMC) from the rat aorta and C7-MDCK ..
  42. ncbi Direct detection of potassium cations bound to G-quadruplex structures by solid-state 39K NMR at 19.6 T
    Gang Wu
    Department of Chemistry, Queen s University, Kingston, Ontario, Canada K7L 3N6
    J Am Chem Soc 125:7182-3. 2003
    ..Solid-state 39K NMR spectra for hydrated K salts of adenosine 2'-monophosphate and adenosine 5'-diphosphate are also reported...
  43. ncbi Kinetic analysis of the effects of monovalent cations and divalent metals on the activity of Mycobacterium tuberculosis alpha-isopropylmalate synthase
    Luiz Pedro S de Carvalho
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Arch Biochem Biophys 451:141-8. 2006
    ..The features of monovalent cation and divalent metal activation, as well as the inhibition by Zn2+ and Cd2+, are discussed in light of the kinetic and structural information available for MtIPMS and other relevant enzymes...
  44. ncbi Influence of monovalent cations on rat alpha- and beta-parvalbumin stabilities
    M T Henzl
    Department of Biochemistry, University of Missouri Columbia, Columbia, Missouri 65211, USA
    Biochemistry 39:5859-67. 2000
    ..Significantly, however, these results do not completely explain the paradoxical stability of the beta isoform, which maintains its higher melting temperature under all conditions examined...
  45. pmc Existence of efficient divalent metal ion-catalyzed and inefficient divalent metal ion-independent channels in reactions catalyzed by a hammerhead ribozyme
    Jing min Zhou
    Gene Discovery Research Center, National Institute of Advanced Industrial Science and Technology AIST, Central 4, 1 1 1 Higashi, Tsukuba Science City 305 8562, Japan
    Nucleic Acids Res 30:2374-82. 2002
    ..Moreover, the observed kinetic data are consistent with Lilley's and DeRose's two-phase folding model that was based on ground state structure analyses...
  46. ncbi Crystal structure of the sodium-potassium pump
    J Preben Morth
    Centre for Membrane Pumps in Cells and Disease PUMPKIN, Danish National Research Foundation, University of Aarhus, Gustav Wieds Vej 10C, DK 8000 Aarhus C, Denmark
    Nature 450:1043-9. 2007
    ..The carboxy terminus of the alpha-subunit is contained within a pocket between transmembrane helices and seems to be a novel regulatory element controlling sodium affinity, possibly influenced by the membrane potential...
  47. ncbi K+, Na+, Mg2+, Ca2+, and water contents in human skeletal muscle: correlations among these monovalent and divalent cations and their alterations in K+ -depleted subjects
    Ratree Tavichakorntrakool
    Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
    Transl Res 150:357-66. 2007
    ..Our study not only provided the descriptive data but also implied the balance or homeostasis of these monovalent and divalent cations in their muscle pools...
  48. ncbi A recombinant plant natriuretic peptide causes rapid and spatially differentiated K+, Na+ and H+ flux changes in Arabidopsis thaliana roots
    Ndiko Ludidi
    University of the Western Cape, Department of Biotechnology, Private Bag X17, Bellville, 7535, South Africa
    Plant Cell Physiol 45:1093-8. 2004
    ..These observations suggest that responses to AtPNP-A are developmental stage and tissue specific and point to a complex role in plant growth and homeostasis...
  49. ncbi Difference in substrate specificity divides the yeast alkali-metal-cation/H(+) antiporters into two subfamilies
    Olga Kinclová
    Department of Membrane Transport, Institute of Physiology CzAcadSci, 14220 Prague 4, Czech Republic
    Microbiology 148:1225-32. 2002
    ..rouxii...
  50. ncbi Cation-pi interactions: structures and energetics of complexation of Na+ and K+ with the aromatic amino acids, phenylalanine, tyrosine, and tryptophan
    Chunhai Ruan
    Department of Chemistry, Wayne State University, Detroit, Michigan 48202, USA
    J Am Chem Soc 126:14600-10. 2004
    ....
  51. pmc Macrocyclic and helical oligoamides as a new class of G-quadruplex ligands
    Pravin S Shirude
    University Chemical Laboratory, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    J Am Chem Soc 129:11890-1. 2007
  52. pmc Regulation of cation transport in Saccharomyces cerevisiae by the salt tolerance gene HAL3
    A Ferrando
    Instituto de Biologia Molecular y Celular de Plantas, , Spain
    Mol Cell Biol 15:5470-81. 1995
    ..Therefore, HAL3 influences cell cycle control and ion homeostasis, acting in parallel to the protein phosphatases Sit4p and calcineurin...
  53. ncbi Minimalist molecular model for nanopore selectivity
    Mauricio Carrillo-Tripp
    Facultad de Ciencias, Universidad Autonoma del Estado de Morelos, Avenida Universidad 1001, 62210 Cuernavaca, Morelos, Mexico
    Phys Rev Lett 93:168104. 2004
    ..This is relevant to the molecular basis of ion selective nanopores and since this mechanism does not depend on the molecular details of the pore, it could also operate in all sorts of nanotubes, from biological to synthetic...
  54. ncbi Characterization of a novel insect digestive DNase with a highly alkaline pH optimum
    Johann P Schernthaner
    Department of Chemistry, University of Ottawa, K1N 6N5, Ottawa, ON, Canada
    Insect Biochem Mol Biol 32:255-63. 2002
    ..to pancreatic DNase I in that divalent alkaline metal ion is required for activity and it is inhibited by monovalent cations. In particular, Mg(2+) and Ca(2+) were the most effective activators...
  55. ncbi The role of Li+, Na+, and K+ in the ligand binding inside the human acetylcholinesterase gorge
    Gabriele Petraglio
    Computational Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich, USI Campus, CH 6900 Lugano, Switzerland
    Proteins 70:779-85. 2008
    ..The combination of computational and biochemical experiments clearly showed that Li(+), Na(+), and K(+) may influence the ligand binding at the hAChE gorge...
  56. ncbi Metal ion requirements for structure and catalysis of an RNA ligase ribozyme
    Margaret E Glasner
    Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
    Biochemistry 41:8103-12. 2002
    ..Ligation rates of the prefolded ribozyme were directly measured and proceed at 800 min(-1) at pH 9.0...
  57. pmc DNA and its counterions: a molecular dynamics study
    Peter Varnai
    Laboratoire de Biochimie Theorique, CNRS UPR 9080, Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie, Paris 75005, France
    Nucleic Acids Res 32:4269-80. 2004
    ..The ion-specific conformational properties of DNA, hitherto largely overlooked, may play an important role in DNA recognition and binding...
  58. pmc Redesigning the monovalent cation specificity of an enzyme
    Swati Prasad
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, Box 8231, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 100:13785-90. 2003
    ..The conversion, however, does not result into a K+-activated enzyme...
  59. ncbi Mechanistic characterization of the HDV genomic ribozyme: classifying the catalytic and structural metal ion sites within a multichannel reaction mechanism
    Shu ichi Nakano
    Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Biochemistry 42:2982-94. 2003
    ....
  60. ncbi Probing Ca2+-induced conformational changes in porcine calmodulin by H/D exchange and ESI-MS: effect of cations and ionic strength
    Mei M Zhu
    Department of Chemistry, Washington University, St Louis, Missouri 63130, USA
    Biochemistry 42:15388-97. 2003
    ....
  61. ncbi Interaction of cyclic cytosine-, guanine-, thymine-, uracil- and mixed guanine-cytosine base tetrads with K+, Na+ and Li+ ions -- a density functional study
    Michael Meyer
    Revotar Biopharmaceuticals AG, Neuendorfstrasse 24a, D 16761, Hennigsdorf, Germany
    J Biomol Struct Dyn 20:507-17. 2003
    ..Uracil and thymine tetrads show a significant different characteristics which may contribute to the differences between DNA and RNA..
  62. ncbi Identification of potential regulatory sites of the Na+,K+-ATPase by kinetic analysis
    Benjamin Y Kong
    School of Chemistry, University of Sydney, Sydney, New South Wales 2006, Australia
    Biochemistry 43:2241-50. 2004
    ....
  63. ncbi The schiff base of gossypol with 3,6,9,12,15,18,21,24-octaoxa-pentacosylamine complexes and monovalent cations studied by electrospray ionization-mass spectrometry, (1)H nuclear magnetic resonance, Fourier transform infrared, as well as PM5 semiempirical
    Piotr Przybylski
    Faculty of Chemistry, A Mickiewicz University, Grunwaldzka 6, 60 780 Poznan, Poland
    Biopolymers 74:273-86. 2004
    ..A Schiff base of gossypol with 3,6,9,12,15,18,21,24-octaoxa-pentacosylamine (GSOB) forms stable complexes with monovalent cations. This process of complex formation was studied by electrospray ionization-mass spectrometry, (1)H-NMR and ..
  64. ncbi Influence of monovalent cation identity on parvalbumin divalent ion-binding properties
    Michael T Henzl
    Department of Biochemistry, University of Missouri Columbia, Columbia, Missouri 65211, USA
    Biochemistry 43:2747-63. 2004
    ..8 x 10(6) M(-1) and the latter to 1.4 x 10(4) and 2.9 x 10(2) M(-1). These data suggest that parvalbumin divalent ion affinity, particularly that of rat alpha, can be significantly attenuated by increased intracellular Na(+) levels...
  65. ncbi Effects of type of added salt and ionic strength on physicochemical and functional properties of casein isolates produced by electroacidification
    Laurent Bazinet
    Centre de recherche et de développement sur les aliments, Agriculture et Agro alimentaire Canada, 3600 Boulevard Casavant Ouest, St Hyacinthe, Quebec, Canada J2S 8E3
    J Agric Food Chem 50:6875-81. 2002
    ..From results on mineral concentrations, it appeared that the addition of monovalent cations did not influence the retention of monovalent or divalent cations in the BMEA isolates, while addition of ..
  66. ncbi Density functional theory investigations on the chemical basis of the selectivity filter in the K+ channel protein
    Fuqiang Ban
    Department of Chemistry, Dalhousie University, Halifax, NS, Canada B3H 4J3
    J Am Chem Soc 126:4711-6. 2004
    ..Overall, the results suggest that the rigid C(4) symmetric selectivity filter is specifically designed for conduction of K(+) ions...
  67. pmc Crystal Structure of human pyridoxal kinase: structural basis of M(+) and M(2+) activation
    Faik N Musayev
    Department of Medicinal Chemistry, School of Pharmacy and Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, Virginia 23219, USA
    Protein Sci 16:2184-94. 2007
    ..Other monovalent cations, Li(+), Cs(+), and Rb(+) do not show significant activity...
  68. ncbi Control of ion selectivity in LeuT: two Na+ binding sites with two different mechanisms
    Sergei Y Noskov
    Institute for Biocomplexity and Informatics, Department for Biological Sciences, University of Calgary, 2500 University Drive, Calgary, AB, Canada
    J Mol Biol 377:804-18. 2008
    ....
  69. ncbi Structural and energetic analysis of metal ions essential to SRP signal recognition domain assembly
    Robert T Batey
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University, New Haven, CT 06520, USA
    Biochemistry 41:11703-10. 2002
    ..Thus, while these metal ions are highly ordered and essential for the formation and stability of the SRP complex, they behave like nonspecific metal ions...
  70. ncbi Effect of metal ion and water coordination on the structure of a gas-phase amino acid
    R A Jockusch
    Department of Chemistry, University of California, Berkeley, CA 94720-1460, USA
    J Am Chem Soc 123:12255-65. 2001
    ..Extensive modeling provides strong support for the validity of inferring structural information from the kinetic data...
  71. ncbi Is there a difference in metal ion-based inhibition between members of thionin family: Molecular dynamics simulation study
    Svetlana Oard
    LSU AgCenter Biotechnology Laboratory, Louisiana State University, 115 Wilson Bldg, LSU, Baton Rouge, LA 70803, USA
    Biophys Chem 130:65-75. 2007
    ..Comparison of MD simulations of alpha-hordothionin with beta-purothionin reveals dynamic properties which we believe are intrinsic properties of thionins with four disulphide bonds...
  72. pmc Definitive assignment of proton selectivity and attoampere unitary current to the M2 ion channel protein of influenza A virus
    T I Lin
    , , D-10098 Berlin, Germany
    J Virol 75:3647-56. 2001
    ..The stringent proton selectivity of M2 suggests that the cytopathology of influenza virus does not involve direct perturbation of cellular sodium or potassium gradients...
  73. ncbi Membrane-bound pyrophosphatase of Thermotoga maritima requires sodium for activity
    Georgiy A Belogurov
    Department of Biochemistry and Food Chemistry, University of Turku, FIN 20014 Turku, Finland
    Biochemistry 44:2088-96. 2005
    ..These results demonstrate that T. maritima PPase belongs to a previously unknown subfamily of Na(+)-dependent H(+)-PPase homologues and may be an analogue of Na(+),K(+)-ATPase...
  74. ncbi Kinetic studies of the effects of K+, Na+ and Li+ on the catalytic activity of human erythrocyte pyridoxal kinase
    P Laine-Cessac
    Laboratoire de Pharmacologie et Toxicologie, Centre Hospitalier Universitaire, Angers, France
    Enzyme Protein 49:291-304. 1996
    ..Results suggest that K+ and Na+ bind to PK on the same site while Li+ binds on another site. This hypothesis and the mechanism of monovalent cation-PK interaction are compared to other well-known K(+)-activated enzymes...
  75. ncbi Attachment of alkali cations on beta-D-glucopyranose: matrix-assisted laser desorption/ionization time-of-flight studies and ab initio calculations
    E Botek
    ICOA, UMR-CNRS 6005, , BP 6759, , France
    Rapid Commun Mass Spectrom 15:273-6. 2001
    ..Finally, the calculated formation enthalpies of the different complexes agree with the experimental results obtained for the order of stability of these complexes...
  76. ncbi Proteolytic activity of the ATP-dependent protease HslVU can be uncoupled from ATP hydrolysis
    H Huang
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 272:21364-72. 1997
    ..However, AMP-PNP binding together with K+ can induce a form of HslVU that degrades proteins without energy consumption...
  77. ncbi Cross-links of quadruplex structures from human telomeric DNA by dinuclear platinum complexes show the flexibility of both structures
    Isabelle Ourliac-Garnier
    Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques, UMR8601, Universite Rene Descartes, 45 Rue des Saints Peres, 75270 Paris Cedex 06, France
    Biochemistry 44:10620-34. 2005
    ..Our results also suggest that the human telomere sequence could be a target for such platinum complexes...
  78. ncbi EPR spectroscopic analysis of U7 hammerhead ribozyme dynamics during metal ion induced folding
    Thomas E Edwards
    Department of Chemistry, University of Washington, Seattle, Washington 98195 1700, USA
    Biochemistry 44:12870-8. 2005
    ..In summary, these results demonstrate that changes in local dynamics, as detected by EPR spectroscopy, can be used to study conformational changes associated with RNA folding and function...
  79. pmc Flow-through lipid nanotube arrays for structure-function studies of membrane proteins by solid-state NMR spectroscopy
    Eduard Y Chekmenev
    The Center for Interdisciplinary Magnetic Resonance, National High Magnetic Field Laboratory, Tallahassee, Florida 32310, USA
    Biophys J 91:3076-84. 2006
    ..We believe that a large number of solid-state NMR-based studies, including structure-function, drug screening, proton exchange, pH, and other titration experiments, will benefit significantly from the method described here...
  80. pmc A trapped intracellular cation modulates K+ channel recovery from slow inactivation
    Evan C Ray
    Department of Physiology, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Gen Physiol 128:203-17. 2006
    ..We propose that the rate of recovery from slow inactivation is determined by the ability of entrapped cations to move into a binding site in the channel's selectivity filter, and refilling of this site is required for recovery...
  81. ncbi Interactions of atmospheric deposition with coniferous canopies in Estonia
    K Pajuste
    Institute of Geography, University of Tartu, Vanemuise, Estonia
    Environ Monit Assess 112:177-96. 2006
    ..6-2.0 and 0.6-1.2 kg ha(-1) per year in spruce and pine stands, respectively. The internal circulation of K(+) was significantly higher (8.9-10.9 kg ha(-1) per year) in spruce stands than in pine stands (2.7-4.4 kg ha(-1) per year)...
  82. ncbi Sodium inhibits permeability transition by decreasing potassium matrix content in rat kidney mitochondria
    Noemí García
    Departamento de Bioquimica, Instituto Nacional de Cardiologia, Ignacio Chavez, Juan Badiano No 1, Col Seccion XVI, Tlalpan, DF, Mexico, Mexico
    Comp Biochem Physiol B Biochem Mol Biol 144:442-50. 2006
    ..It is concluded that the protection provided by Na+ was accomplished by inducing matrix K+ depletion, which, in turn, diminished the free fraction of matrix Ca2+...
  83. ncbi Monovalent cation activation in Escherichia coli inosine 5'-monophosphate dehydrogenase
    K M Kerr
    Department of Biochemistry, Brandeis University, Waltham, Massachusetts, 02454, USA
    Arch Biochem Biophys 375:131-7. 2000
    ..Alternatively, this mutation could uncover a second monovalent cation binding site...
  84. ncbi [A third Na+ binding site in the sodium pump]
    Jean Daniel Horisberger
    Département de Pharmacologie et de Toxicologie, Faculté de Biologie et Médecine, Universite de Lausanne, Suisse
    Med Sci (Paris) 22:27-8. 2006
  85. ncbi Ion selectivity of the cytoplasmic binding sites of the Na,K-ATPase: II. Competition of various cations
    A Schneeberger
    Department of Biology, University of Konstanz, Germany
    J Membr Biol 179:263-73. 2001
    ..The second type of binding site is specific for monovalent cations. its binding affinity is higher than that of the first type, for Na+ ions the equilibrium dissociation ..
  86. ncbi Altering the intermediate in the equilibrium folding of unmodified yeast tRNAPhe with monovalent and divalent cations
    V M Shelton
    Department of Chemistry, Department of Biochemistry and Molecular Biology, and Institute for Biophysical Dynamics, University of Chicago, Illinois 60637, USA
    Biochemistry 40:3629-38. 2001
    ..These results demonstrate that formation of the RNA secondary structure can be independent or coupled to the formation of the tertiary structure depending on their relative stability in monovalent and divalent ions...
  87. ncbi [Effect of lithium hydroxybutyrate on the circadian dynamics of the urinary excretion of Li(+), Na(+), K(+), and Ca(2+) in rats depending on the circadian phase of the drug administration]
    T A Zamoshchina
    Pharmacology Department, Siberian Medical University, Moskovskii trakt 2, Tomsk, 634050 Russia
    Eksp Klin Farmakol 64:21-6. 2001
    ..Upon the morning treatment, lithium cations are excreted faster than after the evening injections...
  88. ncbi The effect of mono- and divalent cations on Tetrahymena thermophila telomeric repeat fragment. A photon correlation spectroscopy study
    Agnieszka Włodarczyk
    Faculty of Physics, Molecular Biophysics Division, A Mickiewicz University, Poznan, Poland
    Acta Biochim Pol 51:971-81. 2004
    ..The effect of Sr(2+) ions on the structures formed was different than that of the monovalent ions. The results obtained in the presence of Sr(2+) could not be described as a function of the number of ions per DNA molecule...
  89. ncbi K(+)/Na(+) selectivity of the KcsA potassium channel from microscopic free energy perturbation calculations
    V B Luzhkov
    Department of Cell and Molecular Biology, Uppsala University, BMC, Box 596, S-751 24 Uppsala, Sweden
    Biochim Biophys Acta 1548:194-202. 2001
    ..The only site that is not K(+) selective is that which is located at the entrance to the internal water cavity, suggesting the possibility that internal Na(+) could block outward currents...
  90. pmc Tuning ion coordination architectures to enable selective partitioning
    Sameer Varma
    Computational Bioscience Department, Sandia National Laboratories, Albuquerque, New Mexico 87185, USA
    Biophys J 93:1093-9. 2007
    ..Specific perturbations to the local binding site environment with respect to strongly selective K-channels result in altered K+/Na+ selectivities...
  91. pmc Probing Na(+)-induced changes in the HIV-1 TAR conformational dynamics using NMR residual dipolar couplings: new insights into the role of counterions and electrostatic interactions in adaptive recognition
    Anette Casiano-Negroni
    Department of Chemistry, Biophysics Research Division, and Program in Bioinformatics, The University of Michigan, 930 North University Avenue, Ann Arbor, Michigan 48109 1055, USA
    Biochemistry 46:6525-35. 2007
    ..Our results underscore the intricate sensitivity of RNA conformational dynamics to environmental conditions and demonstrate the ability to detect subtle conformational changes using NMR RDCs...
  92. ncbi [On the stoichiometry of intercellular Na+/K(+)-exchange]
    I G Gerasimov
    Biofizika 52:69-74. 2007
    ..The different degree of hydratation of Na+ and K+ caused by different density of their charges is considered to be the main reason for the result obtained...
  93. pmc Biochemical and genetic analyses of the role of yeast casein kinase 2 in salt tolerance
    E de Nadal
    Departamento Bioquímica i Biologia Molecular, Universitat Autonoma de Barcelona, Bellaterra 08193, Barcelona, Spain
    J Bacteriol 181:6456-62. 1999
    ..The possible causes for the salt sensitivity phenotype of casein kinase mutants are discussed in the light of these findings...
  94. ncbi Ionic-strength dependence of the conformational change in the unphosphorylated sodium pump
    Shwu Hwa Lin
    CURE Digestive Diseases Research Center, Department of Medicine, School of Medicine, University of California at Los Angeles, Los Angeles, California 90073, USA
    Biochemistry 44:1482-94. 2005
    ..Electrostatic interactions between domains evidently contribute to the driving force for the change from Na+ to K+ conformation and to the stability of the K+ conformation...
  95. ncbi Ion permeation through the Na+,K+-ATPase
    Nicolas Reyes
    Laboratory of Cardiac Membrane Physiology, The Rockefeller University, New York, New York 10021, USA
    Nature 443:470-4. 2006
    ..Close structural homology among the catalytic subunits of Ca2+-, Na+,K+- and H+,K+-ATPases argues that their extracytosolic cation exchange pathways all share these physical characteristics...
  96. ncbi Potassium and sodium ions in a potassium channel studied by molecular dynamics simulations
    P C Biggin
    Department of Biochemistry, University of Oxford, UK
    Biochim Biophys Acta 1510:1-9. 2001
    ..This suggests that the key to the ion selectivity of KcsA is the greater dehydration energy of Na(+) ions, and helps to explain the block of KcsA by internal Na(+) ions...
  97. ncbi Conduction of monovalent and divalent cations in the slow vacuolar channel
    I I Pottosin
    Centro Universitario de Investigaciones Biomedicas, Av. 25 de Julio s/n, Apdo Postal/P.O. Box #199, 28047 Colima, Mexico
    J Membr Biol 181:55-65. 2001
    ....
  98. pmc Cations and hydration in catalytic RNA: molecular dynamics of the hepatitis delta virus ribozyme
    Maryna V Krasovska
    Institute of Biophysics, Academy of Sciences of the Czech Republic, 61265 Brno, Czech Republic
    Biophys J 91:626-38. 2006
    ..b>Monovalent cations bind to the active site and elsewhere assisted by structurally bridging long-residency water molecules, but ..
  99. ncbi Role of fluidity of membranes on the guanyl nucleotide-dependent binding of cholecystokinin-8S to rat brain cortical membranes
    A Rinken
    Institute of Chemical Physics, University of Tartu, Estonia
    Biochem Pharmacol 55:423-31. 1998
    ..5-7.0. The divalent cations Mg2+ and Ca2+ clearly enhanced [3H]CCK-8S binding, whereas the monovalent cations Na+ and K+ were inhibitors...
  100. ncbi Application of a green manure and green manure composted with beet vinasse on soil restoration: effects on soil properties
    M Tejada
    Departamento de Cristalografía, Mineralogía y Química Agrícola, EUITA Universidad de Sevilla, Crta de Utrera km 1, 41013 Sevilla, Spain
    Bioresour Technol 99:4949-57. 2008
    ..7%, 42.7%, and 65.6%, respectively with respect to the control soil), probably because high quantities of monovalent cations (Na principally) were introduced into the soil by the vinasse, thus destabilizing its structure...
  101. pmc A patch clamp study of the nicotinic acetylcholine receptor of bovine adrenomedullary chromaffin cells in culture
    J M Nooney
    Department of Pharmacology and Clinical Pharmacology, Ninewells Hospital, University of Dundee
    J Physiol 455:503-27. 1992
    ..Using isotonic substitutions, the permeability ratios (relative to Na+) for monovalent cations were determined to be 1.32 +/- 0.02 for Cs+ (n = 11), 1.03 +/- 0.02 for Li+ (n = 8) and 0.18 +/- 0...

Research Grants50

  1. TRIC, a Novel Modulator of Intracellular Ca Homeostasis
    Jianjie Ma; Fiscal Year: 2012
    ..Although channels selective for monovalent cations have been reported in SR or ER membranes, no gene has been identified that encodes a SR or ER localized ..
  2. Studies on NikD, a Nikkomycin Biosynthetic Enzyme
    MARILYN S JORNS; Fiscal Year: 2010
    ..We propose to evaluate the catalytic role of the "aromatic cage" and monovalent cations. We will determine whether P2C degradation under physiological conditions is prevented by direct transfer of ..
  3. Studies on Na+ binding to thrombin
    Enrico Di Cera; Fiscal Year: 2010
    ..and prothrombotic functions and will impact the study of clotting enzymes and enzymes activated by monovalent cations in general...
  4. Dynamic ion selectivity of K2P channels and paradoxical depolarization
    Haijun Chen; Fiscal Year: 2013
    ..How K+ channels select K+ over other monovalent cations still remains an unsolved question...
  5. REACTION MECHANISMS FOR ENZYMES
    JULES SHAFER; Fiscal Year: 1990
    ..D-Serine dehydratase from E. coli will be studied to determine a) how monovalent cations effect the affinity of the enzyme for its cofactor pyridoxal 5'-phosphate, b) the involvement of a thiol ..
  6. Mechanisms of serum electrolyte effects on block of HERG
    Alan Miller; Fiscal Year: 2006
    ..either wild type HERG channels or mutant HERG channels to study the mechanisms by which the extracellular monovalent cations potassium, rubidium, and cesium and the divalent cations calcium and magnesium alter block of HERG by a ..
  7. RELATIONSHIP BETWEEN PROTEIN STRUCTURE AND FUNCTION
    George Hess; Fiscal Year: 2006
    ..new photolabile precursors of the neurotransmitters GABA and serotonin and of an anionic indicator for monovalent cations. Caged GABA will be used to determine the difference in the mechanism between normal GABA(A) receptors and a ..
  8. ALLOSTERIC REGULATION OF SUBSTRATE CHANNELING
    Michael Dunn; Fiscal Year: 2006
    ..b) The roles played by protein conformational dynamics in biological function, (c) the roles of monovalent cations in the modulation of protein function, (d) the role played by substrates as allosteric effectors in ..
  9. Subconductance behavior in T-type calcium channels
    KATIE BITTNER; Fiscal Year: 2009
    ..each conductance state will be measured in both the presence of different permeant cations, Ca, barium, and monovalent cations. In the second aim quantitative differences between CaV 3.1 and CaV 3.2 will be assessed...
  10. DIFFRACTION ANALYSIS OF FRUCTOSE-1,6-DIPHOSPHATASE
    CHARLES WEEKS; Fiscal Year: 1980
    ..with a variety of allosteric effectors including AMP, activating and inhibitory divalent cations, and monovalent cations. The studies proposed here will pave the way for eventual X-ray analysis of enzyme-substrate, enzyme-..
  11. MOLECULAR AND FUNCTIONAL ANALYSIS OF ATP RECEPTORS
    MARK VOIGT; Fiscal Year: 2003
    ..These receptors are ligand-gated channels that are permeable not only to monovalent cations such as Na+ and K+, but also to Ca2+...
  12. DIFFRACTION ANALYSIS OF FRUCTOSE-1,6-DIPHOSPHATASE
    Alexander McPherson; Fiscal Year: 1980
    ..with a variety of allosteric effectors including AMP, activating and inhibitory divalent cations, and monovalent cations. The studies proposed here will pave the way for eventual X-ray analysis of enzyme-substrate, enzyme-..
  13. ION PERMEATION IN CHANNELS, CARRIERS, AND NERVES
    GEORGE EISENMAN; Fiscal Year: 1991
    ..For artificial channels, we propose to continue studies on selectivity among monovalent cations for the Gramicidin A channel, extending these to theoretical investigations of multi-site, multi-barrier ..
  14. CELLULAR BASIS OF TUNING IN THE COCHLEA
    JONATHAN ART; Fiscal Year: 1992
    ..currents in cells of known characteristic frequency will be analyzed using intracellular exchange of monovalent cations. Simultaneous cell-attached single-channel and perforated-patch whole cell recordings will be used to ..
  15. THIN LAYER CHANNEL MEMBRANES FOR SENSOR APPLICATIONS
    HARRIS GOLD; Fiscal Year: 1990
    ..of the monolayers and the helix conformation of gramicidin within the monolayer to the transport of monovalent cations through the channel...
  16. HOST-VIRUS INTERACTIONS IN INITIATION OF DNA REPLICATION
    Roger McMacken; Fiscal Year: 2003
    ..ii) the affinity of nucleotides for DnaK, or (iii) small molecule effectors such as inorganic phosphate and monovalent cations. In a parallel approach, we will construct a series of site-specific DnaK and DnaJ substitution mutants that ..
  17. HORMONAL REGULATION OF MITOCHONDRIAL FUNCTION
    RUSSELL YAMAZAKI; Fiscal Year: 1980
    ..The effect of varying monovalent cations in the assay medium used for respiratory studies will be determined...
  18. MODIFICATION OF TUMOR CELLS AND TUMOR SYSTEMS
    Martin Lubin; Fiscal Year: 1980
    ..of non-tumor cells is limited or controlled, under physiological conditions, by the levels of intracellular monovalent cations or the activity of the Na/K ATPase pump system...
  19. A MODEL FOR CALCIUM HOMEOSTASIS IN EXCITABLE TISSUE
    MARGUERITE STOUT; Fiscal Year: 1980
    ..gradient, of ATP, of potential difference, of temperature, of pH and of the substitution of divalent and monovalent cations upon calcium influx and efflux will be investigated...
  20. MECHANISMS OF PYRIDOXAL-5'-PHOSPHATE DEPENDENT ENZYMES
    Robert Phillips; Fiscal Year: 2004
    ..Thus the results of this research are likely to make a significant contribution to the goal of improving health care for Americans in the future. ..
  21. Multiplexed mRNA quanitification using self-circularizing RNA probes
    SERGEI KAZAKOV; Fiscal Year: 2008
    ..This system will be particularly useful for RNAi-based drugs that are currently in development for several disorders, including HCV, where the ability to monitor target knock-down and off-target effects is important. [unreadable]..
  22. Modeling of Ion Pores
    Peter Jordan; Fiscal Year: 2007
    ..A new, efficient way to treat membrane-mediated influences between a channel's transmembrane segments is outlined. It will be validated on alamethecin and used to better characterize gating of the MscL channel. ..
  23. Structures of the ends of telomeres
    MARTIN HORVATH; Fiscal Year: 2007
    ..Insights obtained from the proposed research will thus have significance for understanding human telomeres and the role that telomere structure plays in the biology of aging and cancer. [unreadable] [unreadable]..
  24. Ion Regulation of Kv Channel Gating and Permeation
    Carol Deutsch; Fiscal Year: 2007
    ..The second aim will test whether ions in the selectivity filter modulate the movement of voltage sensors. These studies will entail measurements of kinetics and voltage dependence of gating currents in HEK cells. ..
  25. STRUCTURE FUNCTION & BIOSYNTHESIS OF RESPIRATORY ENZYMES
    VICTOR DAVIDSON; Fiscal Year: 2008
    ..abstract_text> ..
  26. Dynamics, Function, and Stability in Large Enzymes
    JOSEPH LORIA; Fiscal Year: 2009
    ..This combination of experiments will allow characterization of the dynamics of catalytically important residues and the active site loop. ..
  27. Probing the open channel state of AchR
    JOHN LEITE; Fiscal Year: 2003
    ..These experiments should identify structural elements involved in gating mechanism and should yield a model for the gating mechanism. ..
  28. A new method for multiplex detection of micro-RNAs (miR-ID)
    SERGEI KAZAKOV; Fiscal Year: 2008
    ..The method of micro-RNA detection has novel features that promise better sensitivity and selectivity than other micro-RNA assays currently available. [unreadable] [unreadable] [unreadable] [unreadable]..
  29. Directed Libraries of Self-Circularizing Antisense RNA
    SERGEI KAZAKOV; Fiscal Year: 2004
    ..They should find diverse applications, includinginhibitingthe expression of disease-related genes, functional genomics, and target validation. ..
  30. Expression, function and regulation of renal claudins
    ALAN S YU; Fiscal Year: 2010
    ..We hypothesize that monovalent cations permeate the pore by partially dehydrating and are then stabilized either by discrete electrostatic binding ..
  31. Oligopyrrole-based Anion Binding Agents
    Jonathan L Sessler; Fiscal Year: 2010
    ..abstract_text> ..
  32. Enhancing Successful Post-Baccalaureate Intervention to Promote Research Careers
    Terry Ann Krulwich; Fiscal Year: 2010
    ..The studies test hypothesized factors as predictors/builders of successful PhD work and test the adaptability of successful PREP interventions to a doctoral program setting. ..
  33. FASEB Conference on Calcium and Cell Function
    Richard Lewis; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  34. BIOSYNTHESIS OF SOME MICROBIAL METABOLITES
    Ronald Parry; Fiscal Year: 2007
    ..Future investigations of sparsomycin will focus on cloning the sparsomycin resistance gene from S. sparsogenes to understand the mechanism of self-resistance in this organism and to gain access to the biosynthetic genes. ..
  35. Library-based selection of optimal gene inhibitors
    SERGEI KAZAKOV; Fiscal Year: 2003
    ..In this way, optimized Lassos will be derived in an automatic and straightforward manner for application as agents for target validation, gene function analysis, and therapeutics. ..
  36. Bacillus anthracis Spores: Initiation of Germination
    TERRY KRULWICH; Fiscal Year: 2003
    ..Such transporters may be new virulence factors, i.e. additional potential therapeutic targets. ..
  37. Amplification of Custom RNA in Plants
    SERGEI KAZAKOV; Fiscal Year: 2002
    ..Our current business strategy is to sell custom RNA in partnership with Large Scale Biology Corp. and license the technology to certain other manufacturers. ..
  38. FREE SOLUTION MOBILITY AS A PROBE OF DNA ELECTROSTATICS
    Nancy Stellwagen; Fiscal Year: 2004
    ..Further work entails an examination of the interaction between DNA and commonly used buffers in electrophoresis experiments. ..
  39. Medicinal Inorganic Chemistry ACS Symposium F-2003
    Jonathan Sessler; Fiscal Year: 2003
    ..It is thus expected that the present MIC-ACS symposium will afford a unique opportunity to develop further growth in a field of study that is rich in opportunity and central to the NIH mission. ..
  40. NMDA RECEPTOR--AGONIST AFFINITY, EFFICACY/TRANSDUCTION
    Antonius VanDongen; Fiscal Year: 2004
    ..These studies will result in a detailed molecular picture of the dynamic change in structure that accompany activation of the NMDA receptor. ..
  41. Tet Efflux Proteins: Implications of Multiple Functions
    TERRY KRULWICH; Fiscal Year: 2004
    ..Specific Aim 3 will: clarify the basis for different phenotypes of tet(L) mutants; will further test the novel translational reinitiation model for tet(L) regulation; and will probe additional, Na- and pH related regulation. ..
  42. TRPM4 Channels in the Control of Cerebral Artery Tone
    Scott Earley; Fiscal Year: 2005
    ..Cloned TRPM4b channels expressed in HEK-293 cells are selective for monovalent cations and are activated by mechanical stretch...
  43. GATING AND SELECTIVITY IN C TYPE INACTIVATED K CHANNELS
    John Starkus; Fiscal Year: 2001
    ..Defects in this mechanism are responsible for the LQT1 and LQT2 syndromes associated with potentially lethal cardiac arrhythmias. ..
  44. BIOSYNTHESIS AND METABOLISM OF CYCLOPENTANOIDS
    Ronald Parry; Fiscal Year: 2001
    ..The potential use of the CmaA gene for construction of hybrid peptide antibiotics containing L-alloisoleucine or coronamic acid will also be explored, using surfactin biosynthesis in B. subtilis as a model system. ..
  45. Biophysics of CNS Connexins
    VYTAUTAS VERSELIS; Fiscal Year: 2006
    ..abstract_text> ..
  46. Dendritic Processing in the Olfactory Bulb
    Jason Christie; Fiscal Year: 2003
    ..The specific aims are: (1) to determine the function of autoexcitation in mitral cell processing and (2) to determine how dendritic compartmentalization effects mitral cell processing. ..
  47. Putting Molecular Dynamics to the Test: Ion Permeation
    Olaf Andersen; Fiscal Year: 2008
    ..abstract_text> ..