fluoresceins

Summary

Summary: A family of spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays.

Top Publications

  1. ncbi Fluoro-Jade B: a high affinity fluorescent marker for the localization of neuronal degeneration
    L C Schmued
    Division of Neurotoxicology, National Center for Toxicological Research FDA, 3900 NCTR Road, 72079 9502, Jefferson, AR 72079 9502, USA
    Brain Res 874:123-30. 2000
  2. ncbi Effect of Leucine and Lysine substitution on the antimicrobial activity and evaluation of the mechanism of the HPA3NT3 analog peptide
    Ramamourthy Gopal
    Research Center for Proteineous Materials RCPM, Chosun University, Gwangju, Korea
    J Pept Sci 15:589-94. 2009
  3. ncbi Comparing bacterial membrane interactions of antimicrobial peptides and their mimics
    Nathaniel P Chongsiriwatana
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA
    Methods Mol Biol 618:171-82. 2010
  4. ncbi In vitro P-glycoprotein assays to predict the in vivo interactions of P-glycoprotein with drugs in the central nervous system
    Bo Feng
    Pharmacokinetics, Dynamics, and Metabolism Department, Pfizer Global Research and Development, Groton, Connecticut 06340, USA
    Drug Metab Dispos 36:268-75. 2008
  5. ncbi Negative regulation of neural stem/progenitor cell proliferation by the Pten tumor suppressor gene in vivo
    M Groszer
    Howard Hughes Medical Institute, Department of Molecular and Medical Pharmacology, UCLA School of Medicine, Los Angeles, CA 90095, USA
    Science 294:2186-9. 2001
  6. ncbi YC-1, a novel potential anticancer agent, inhibit multidrug-resistant protein via cGMP-dependent pathway
    Chin Chuan Hung
    Department of Pharmacy, College of Pharmacy, China Medical University, No 91 Hsueh Shih Road, Taichung, Taiwan, 40402, Republic of China
    Invest New Drugs 29:1337-46. 2011
  7. pmc Real-time measurement of nitric oxide in single mature mouse skeletal muscle fibres during contractions
    Deborah Pye
    Division of Metabolic and Cellular Medicine, School of Clinical Sciences, University of Liverpool, Liverpool L69 3GA, UK
    J Physiol 581:309-18. 2007
  8. ncbi Oxidative stress during aging of stationary cultures of the yeast Saccharomyces cerevisiae
    W Jakubowski
    Department of Molecular Biophysics, University of Lodz, Lodz, Poland
    Free Radic Biol Med 28:659-64. 2000
  9. ncbi In vitro p-glycoprotein inhibition assays for assessment of clinical drug interaction potential of new drug candidates: a recommendation for probe substrates
    Jarkko Rautio
    Preclinical Drug Metabolism and Pharmacokinetics, GlaxoSmithKline, Inc, Research Triangle Park, NC 27709, USA
    Drug Metab Dispos 34:786-92. 2006
  10. ncbi Methods to measure the reactivity of peroxynitrite-derived oxidants toward reduced fluoresceins and rhodamines
    Peter Wardman
    University of Oxford, Gray Cancer Institute, Mount Vernon Hospital, Northwood, Middlesex, United Kingdom
    Methods Enzymol 441:261-82. 2008

Research Grants

  1. Novel Extended Red-Shifted Rhodamine Dyes
    NICHOLAS CAIRNS; Fiscal Year: 2004
  2. DEVELOPMENT OF CHLORIDE-SENSITIVE FLUORESCENT INDICATORS
    Alan Verkman; Fiscal Year: 1991
  3. Modeling, Estimation and Control in HIV Dynamics
    H Banks; Fiscal Year: 2005
  4. In Vivo Gene Silencing with siRNA After Brain Trauma
    HELEN HELLMICH; Fiscal Year: 2006
  5. Mineral and Mechanical Properties of Bone
    Sarandeep Huja; Fiscal Year: 2005
  6. GENE EXPRESSION-ENVIRONMENT LINK IN CEREBRAL ISCHEMIA
    TERESITA BRIONES; Fiscal Year: 2006
  7. A THERAPEUTIC MODEL FOR CEREBRAL ISCHEMIA
    TERESITA BRIONES; Fiscal Year: 2003
  8. Antioxidant Functions of Lipoic Acid
    James May; Fiscal Year: 2004
  9. MOLECULAR ANGLE TRANSITIONS IN MUSCLE
    THOMAS BURGHARDT; Fiscal Year: 2001
  10. Antioxidant vitamins in models of Alzheimer's Disease
    James May; Fiscal Year: 2008

Detail Information

Publications267 found, 100 shown here

  1. ncbi Fluoro-Jade B: a high affinity fluorescent marker for the localization of neuronal degeneration
    L C Schmued
    Division of Neurotoxicology, National Center for Toxicological Research FDA, 3900 NCTR Road, 72079 9502, Jefferson, AR 72079 9502, USA
    Brain Res 874:123-30. 2000
    ..Like Fluoro-Jade, Fluoro-Jade B is compatible with a number of other labeling procedures including immunofluorescent and fluorescent Nissl techniques...
  2. ncbi Effect of Leucine and Lysine substitution on the antimicrobial activity and evaluation of the mechanism of the HPA3NT3 analog peptide
    Ramamourthy Gopal
    Research Center for Proteineous Materials RCPM, Chosun University, Gwangju, Korea
    J Pept Sci 15:589-94. 2009
    ..These results suggest that the substitutions of Arg and Asn with Lys and two Trp with Leu resulted in small changes in HPA3NT3-analog activity and significant decreases in hemolytic activity...
  3. ncbi Comparing bacterial membrane interactions of antimicrobial peptides and their mimics
    Nathaniel P Chongsiriwatana
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA
    Methods Mol Biol 618:171-82. 2010
    ..By comparing the membrane interactions of AMPs and their mimics, these techniques can provide insights into their extent of mimicry and their antimicrobial mechanisms...
  4. ncbi In vitro P-glycoprotein assays to predict the in vivo interactions of P-glycoprotein with drugs in the central nervous system
    Bo Feng
    Pharmacokinetics, Dynamics, and Metabolism Department, Pfizer Global Research and Development, Groton, Connecticut 06340, USA
    Drug Metab Dispos 36:268-75. 2008
    ....
  5. ncbi Negative regulation of neural stem/progenitor cell proliferation by the Pten tumor suppressor gene in vivo
    M Groszer
    Howard Hughes Medical Institute, Department of Molecular and Medical Pharmacology, UCLA School of Medicine, Los Angeles, CA 90095, USA
    Science 294:2186-9. 2001
    ..However, cell fate commitments of the progenitors were largely undisturbed. Our results suggest that PTEN negatively regulates neural stem cell proliferation...
  6. ncbi YC-1, a novel potential anticancer agent, inhibit multidrug-resistant protein via cGMP-dependent pathway
    Chin Chuan Hung
    Department of Pharmacy, College of Pharmacy, China Medical University, No 91 Hsueh Shih Road, Taichung, Taiwan, 40402, Republic of China
    Invest New Drugs 29:1337-46. 2011
    ..These results indicated that YC-1 inhibited Pgp efflux via the NO-cGMP-PKG-ERK signaling pathway through noncompetitive inhibition. The present study revealed that YC-1 could be a good candidate for development as a MDR modulator...
  7. pmc Real-time measurement of nitric oxide in single mature mouse skeletal muscle fibres during contractions
    Deborah Pye
    Division of Metabolic and Cellular Medicine, School of Clinical Sciences, University of Liverpool, Liverpool L69 3GA, UK
    J Physiol 581:309-18. 2007
    ....
  8. ncbi Oxidative stress during aging of stationary cultures of the yeast Saccharomyces cerevisiae
    W Jakubowski
    Department of Molecular Biophysics, University of Lodz, Lodz, Poland
    Free Radic Biol Med 28:659-64. 2000
    ..The magnitude of this stress was augmented in antioxidant-deficient strains, devoid of superoxide dismutases and catalases, and of decreased glutathione content...
  9. ncbi In vitro p-glycoprotein inhibition assays for assessment of clinical drug interaction potential of new drug candidates: a recommendation for probe substrates
    Jarkko Rautio
    Preclinical Drug Metabolism and Pharmacokinetics, GlaxoSmithKline, Inc, Research Triangle Park, NC 27709, USA
    Drug Metab Dispos 34:786-92. 2006
    ..Finally, a strategy integrating results from in vitro assays (efflux, inhibition, and ATPase) is provided to further guide clinical interaction studies...
  10. ncbi Methods to measure the reactivity of peroxynitrite-derived oxidants toward reduced fluoresceins and rhodamines
    Peter Wardman
    University of Oxford, Gray Cancer Institute, Mount Vernon Hospital, Northwood, Middlesex, United Kingdom
    Methods Enzymol 441:261-82. 2008
    ..This chapter outlines methods for estimation of key rate constants involving peroxynitrite-derived oxidants...
  11. pmc In situ detection and measurement of intracellular reactive oxygen species in single isolated mature skeletal muscle fibers by real time fluorescence microscopy
    Jesus Palomero
    Division of Metabolic and Cellular Medicine, School of Clinical Sciences, University of Liverpool, Liverpool, United Kingdom
    Antioxid Redox Signal 10:1463-74. 2008
    ..This technique has advantages over previous approaches because of the maturity of the fibers and the analysis of single cells, which prevent contributions from nonmuscle cells...
  12. ncbi Transcutaneous measurement of renal function in conscious mice
    Andrea Schreiber
    Institute of Physiology, University of Regensburg, Regensburg, Germany
    Am J Physiol Renal Physiol 303:F783-8. 2012
    ..In summary, we describe a technique allowing the measurement of renal function in freely moving mice independent of blood or urine sampling as well as of laboratory assays...
  13. ncbi Transgenically encoded protein photoinactivation (FlAsH-FALI): acute inactivation of synaptotagmin I
    Kurt W Marek
    Department of Biochemistry, University of California, San Francisco, 94143, USA
    Neuron 36:805-13. 2002
    ..We demonstrate that Syt I is required for a post-docking step during vesicle fusion but does not function to stabilize the docked vesicle state...
  14. ncbi A division-dependent compartmental model for computing cell numbers in CFSE-based lymphocyte proliferation assays
    H T Banks
    Center for Research in Scientic Computation, Center for Quantitative Sciences in Biomedicine, North Carolina State University, Raleigh, NC 27695 8212, United States
    Math Biosci Eng 9:699-736. 2012
    ..Variability in cellular autofluorescence is found to play a significant role in the data, as well. Finally, the compartmental model is compared to previous work, and statistical aspects of the experimental data are discussed...
  15. pmc Corneal alternations induced by topical application of benzalkonium chloride in rabbit
    Wensheng Chen
    Eye Institute and affiliated Xiamen Eye Center of Xiamen University, Fujian, China
    PLoS ONE 6:e26103. 2011
    ..A high concentration of BAC breaks down the barrier integrity of corneal endothelium, concomitant with the disruption of PAMR and remodeling of apical junctional complex in vivo...
  16. pmc Synthesis of rhodamines from fluoresceins using Pd-catalyzed C-N cross-coupling
    Jonathan B Grimm
    Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia 20147, USA
    Org Lett 13:6354-7. 2011
    ....
  17. pmc Evaluation of multitype mathematical models for CFSE-labeling experiment data
    Hongyu Miao
    Department of Biostatistics and Computational Biology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Box 630, Rochester, NY 14642, USA
    Bull Math Biol 74:300-26. 2012
    ..This model was then employed to understand the proliferation pattern of CD4+ and CD8+ T cells under polyclonal stimulation...
  18. ncbi Increased methylglyoxal and oxidative stress in hypertensive rat vascular smooth muscle cells
    Lingyun Wu
    Department of Anatomy and Cell Biology, College of Medicine, University of Saskatchewan, Saskatoon, Canada
    Hypertension 39:809-14. 2002
    ..Our findings suggest that that elevated methylglyoxal and associated oxidative stress possibly contribute to the pathogenesis of hypertension...
  19. ncbi Detection of reactive oxygen and reactive nitrogen species in skeletal muscle
    C L Murrant
    Department of Pharmacology and Physiology, University of Rochester, Rochester, New York 14642, USA
    Microsc Res Tech 55:236-48. 2001
    ..Eventual delineation of the mechanisms whereby ROS and RNS regulate cellular function will hinge on our understanding of the production and distribution of ROS and RNS within skeletal muscle...
  20. ncbi Skin penetration and deposition of carboxyfluorescein and temoporfin from different lipid vesicular systems: In vitro study with finite and infinite dosage application
    Ming Chen
    Department of Pharmaceutical Technology, Friedrich Schiller University Jena, Lessing str 8, D 07743 Jena, Germany
    Int J Pharm 408:223-34. 2011
    ..The results also revealed that the factors influencing the drug skin distribution concern the physicochemical characteristics of the drug, the choice of the vehicle formulation and the application mode applied...
  21. ncbi The mitochondrial permeability transition in cell death: a common mechanism in necrosis, apoptosis and autophagy
    J J Lemasters
    Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill, CB No 7090, 236 Taylor Hall, Chapel Hill, NC 27799 7090, USA
    Biochim Biophys Acta 1366:177-96. 1998
    ..A model is proposed in which onset of the MPT to increasing numbers of mitochondria within a cell leads progressively to autophagy, apoptosis and necrotic cell death...
  22. pmc Tuberization in potato involves a switch from apoplastic to symplastic phloem unloading
    R Viola
    Unit of Plant Biochemistry, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, United Kingdom
    Plant Cell 13:385-98. 2001
    ..Evidence is presented that the apical and lateral tuber buds function as isolated domains with respect to sucrose unloading and metabolism...
  23. ncbi Continuous perfusion microfluidic cell culture array for high-throughput cell-based assays
    Paul J Hung
    Berkeley Sensor and Actuator Center, Department of Bioengineering, University of California, 485 Evans Hall, Berkeley, CA 94720, USA
    Biotechnol Bioeng 89:1-8. 2005
    ..The cell culture array could offer a platform for a wide range of assays with applications in drug screening, bioinformatics, and quantitative cell biology...
  24. ncbi Reactivity of 2',7'-dichlorodihydrofluorescein and dihydrorhodamine 123 and their oxidized forms toward carbonate, nitrogen dioxide, and hydroxyl radicals
    Marta Wrona
    Gray Cancer Institute, Mount Vernon Hospital, Northwood, Middlesex HA6 2JR, UK
    Free Radic Biol Med 38:262-70. 2005
    ..These data suggest that probe oxidation may often be in competition with reaction of the radicals with cellular antioxidants...
  25. ncbi Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data
    Edwin D Hawkins
    Immunology Division, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria, Australia
    Nat Protoc 2:2057-67. 2007
    ..In this protocol, we present a detailed method for examining time-series data using graphical and computer-based procedures available to all experimenters...
  26. ncbi Evaluation of fluorescent dyes for measuring intracellular glutathione content in primary cultures of human neurons and neuroblastoma SH-SY5Y
    Jordi Sebastia
    Department of Pharmacology and Toxicology, Institut d Investigacions Biomediques de Barcelona, CSIC, IDIBAPS, Barcelona, Spain
    Cytometry A 51:16-25. 2003
    ..To study GSH content in human neuronal cell cultures, thiol-sensitive fluorescent techniques requiring a small number of cells may be of great value, but their GSH specificity has not been established in these cells...
  27. ncbi New and improved methods for measuring lymphocyte proliferation in vitro and in vivo using CFSE-like fluorescent dyes
    Benjamin J C Quah
    Department of Immunology, John Curtin School of Medical Research, Australian National University, Canberra, ACT 2601, Australia
    J Immunol Methods 379:1-14. 2012
    ....
  28. pmc Estimation of cell proliferation dynamics using CFSE data
    H T Banks
    Center for Research in Scientific Computation, North Carolina State University, Raleigh, NC 27695 8212, USA
    Bull Math Biol 73:116-50. 2011
    ..The resulting new cell dynamics model should prove useful in proliferation assay tracking and modeling, with numerous applications in the biomedical sciences...
  29. ncbi Inhibition of HIV-1 Tat-dependent trans activation by steric block chimeric 2'-O-methyl/LNA oligoribonucleotides
    A Arzumanov
    Laboratory of Molecular Biology, Medical Research Council, Hills Road, Cambridge CB2 2QH, UK
    Biochemistry 40:14645-54. 2001
    ..An alternating PS-containing 3'-FAM OMe 12-mer oligonucleotide exhibited partial inhibition of trans-activation activity, but this was correlated with a similar effect on control gene expression, suggesting nonspecific inhibition...
  30. ncbi Use of CFDA-SE for evaluating the in vitro proliferation pattern of human mesenchymal stem cells
    S Urbani
    Hematology Unit, Careggi Hospital, Viale Morgagni 85, 50134 Florence, Italy
    Cytotherapy 8:243-53. 2006
    ....
  31. ncbi Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins
    Carla C Spagnuolo
    Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, CIHIDECAR, CONICET, Ciudad Universitaria Pabellon II, 1428, Buenos Aires, Argentina
    J Am Chem Soc 128:12040-1. 2006
    ..The two probes also provide a new FRET pair with a larger Ro value (54 A) than any previously obtained with biarsenical dyes...
  32. ncbi Identification and characterization of the canine multidrug resistance-associated protein
    Liandong Ma
    Division of Cancer Research, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285, USA
    Mol Cancer Ther 1:1335-42. 2002
    ..Knowledge of species differences between canine and human proteins will aid in the design of appropriate pharmacokinetic and toxicokinetic studies for the preclinical evaluation of MRP1 modulators...
  33. ncbi Cell responses to xenobiotics: comparison of MCF7 multi-drug- and mussel blood cell multi-xenobiotic-defense mechanisms
    Matthieu Marin
    Laboratory of Ecotoxicology, UPRES EA 3222, IFRMP 23, University of Le Havre, BP 540 76058 Le Havre, France
    Mar Environ Res 58:209-13. 2004
    ..We conclude that such microplate assays could be used in primary cultures of MBC to estimate the effects of various chemicals on MXR activity...
  34. ncbi Identification of an orthogonal peptide binding motif for biarsenical multiuse affinity probes
    Baowei Chen
    Cell Biology and Biochemistry Group, Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, USA
    Bioconjug Chem 18:1259-65. 2007
    ....
  35. ncbi Quantifying cell turnover using CFSE data
    Vitaly V Ganusov
    Department of Biology, Emory University, Atlanta, GA 30322, USA
    J Immunol Methods 298:183-200. 2005
    ..We propose that measuring an additional parameter, the fraction of cells in division, may allow estimation of all model parameters including the death rates during different stages of the cell cycle...
  36. ncbi Fast laser-induced solute release from liposomes sensitized with photochromic lipid: effects of temperature, lipid host, and sensitizer concentration
    R H Bisby
    Science Research Institute, University of Salford, Salford, M5 4WT, United Kingdom
    Biochem Biophys Res Commun 262:406-10. 1999
    ..The results presented are relevant to the design of photostimulated drug delivery systems and to potential applications of photosensitive liposomes as caging agents for biological effectors...
  37. ncbi The physiological response of the marine platyhelminth Macrostomum lignano to different environmental oxygen concentrations
    G A Rivera-Ingraham
    Department of Functional Ecology, Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany
    J Exp Biol 216:2741-51. 2013
    ....
  38. ncbi Direct fluorometric analysis of a newly synthesised fluorescein-labelled marker for glomerular filtration rate
    Johannes Pill
    Roche Diagnostics GmbH, Sandhofer Str 116, 68305, Mannheim, Germany
    Anal Bioanal Chem 382:59-64. 2005
    ..The dramatic decrease in analytical effort will result in a significantly higher acceptability of GFR determination...
  39. ncbi Flow cytometric analysis of chronic and acute toxicity of copper(II) on the marine dinoflagellate Amphidinium carterae
    O M Lage
    Departamento de Botânica da Faculdade de Ciências and Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Porto, Portugal
    Cytometry 44:226-35. 2001
    ....
  40. pmc Multiple lipid compartments slow vesicle contents release in lipases and serum
    Cecile Boyer
    Department of Chemical Engineering, University of California, Santa Barbara, CA 93106, USA
    ACS Nano 1:176-82. 2007
    ..The vesosome is a better mimic of eukaryotic cell structure and demonstrates the benefits of multiple internal bilayer-enclosed compartments...
  41. ncbi A general mathematical framework to model generation structure in a population of asynchronously dividing cells
    Kalet Leon
    Centro de Inmunologia Molecular, Habana, Cuba
    J Theor Biol 229:455-76. 2004
    ....
  42. pmc Visualization and enrichment of live putative cancer stem cell populations following p53 inactivation or Bax deletion using non-toxic fluorescent dyes
    Joshua E Allen
    Laboratory of Molecular Oncology and Cell Cycle Regulation, Departments of Medicine Hematology Oncology, Genetics and Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    Cancer Biol Ther 8:2194-205. 2009
    ....
  43. ncbi Analysis and simulation of division- and label-structured population models : a new tool to analyze proliferation assays
    J Hasenauer
    Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum Munchen, Neuherberg, Germany
    Bull Math Biol 74:2692-732. 2012
    ..This is illustrated by modeling and simulating a proliferating population with division number-dependent proliferation rate...
  44. ncbi Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling
    Xiao Qi Wang
    The Central Laboratory, The First Teaching Hospital of Jilin University, Changchun 130021, China
    Acta Biochim Biophys Sin (Shanghai) 37:379-85. 2005
    ....
  45. ncbi Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester
    Ben J C Quah
    Division of Immunology and Genetics, John Curtin School of Medical Research, The Australian National University, Canberra, Australian Capital Territory, Australia
    Nat Protoc 2:2049-56. 2007
    ..An important feature of the technique is that division-dependent changes in the expression of cell-surface markers and intracellular proteins are easily quantified by flow cytometry...
  46. pmc A new model for the estimation of cell proliferation dynamics using CFSE data
    H T Banks
    Center for Research in Scientific Computation, North Carolina State University, Raleigh, NC 27695 8212, USA
    J Immunol Methods 373:143-60. 2011
    ..It is shown that these improvements result in a model with a strong physiological basis which is fully capable of replicating the behavior observed in the data...
  47. ncbi New method to quantify erythrophagocytosis by autologous monocytes
    Rolf Fendel
    Medical Research Unit, Albert Schweitzer Hospital, Lambarene, Gabon
    Cytometry A 71:258-64. 2007
    ..In contrast, erythrophagocytosis by autologous phagocytes is more difficult to quantify...
  48. ncbi Fluorescence microplate assay for the detection of oxidative burst products in tobacco cell suspensions using 2',7'-dichlorofluorescein
    Isak B Gerber
    Department of Biochemistry, RAU University, Auckland Park, Johannesburg, South Africa
    Methods Cell Sci 25:115-22. 2003
    ....
  49. ncbi New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications
    Stephen R Adams
    Department of Pharmacology, Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, and Biomedical Sciences Program, University of California, San Diego, La Jolla, California 92093 0647, USA
    J Am Chem Soc 124:6063-76. 2002
    ..Analogous biarsenicals enable affinity chromatography, fluorescence anisotropy measurements, and electron-microscopic localization of tetracysteine-tagged proteins...
  50. ncbi Up-regulation of multidrug resistance P-glycoprotein via nuclear factor-kappaB activation protects kidney proximal tubule cells from cadmium- and reactive oxygen species-induced apoptosis
    F Thevenod
    Department of Physiology II, University of Saarland, 66421 Homburg, Germany
    J Biol Chem 275:1887-96. 2000
    ..These data suggest that mdr1 up-regulation, at least in part, provides anti-apoptotic protection for PT cells against cadmium-mediated stress...
  51. ncbi Interaction of cationic antimicrobial peptides with model membranes
    L Zhang
    Department of Microbiology and Immunology, University of British Columbia, 300-6174 University Boulevard, Vancouver, British Columbia V6T 1Z3, Canada
    J Biol Chem 276:35714-22. 2001
    ..Overall, whereas all studied cationic antimicrobial peptides interacted with membranes, they were quite heterogeneous in their impact on these membranes...
  52. ncbi Elevation of plasma membrane permeability by laser irradiation of selectively bound nanoparticles
    Cuiping Yao
    Key Laboratory of Biomedical Information Engineering of Ministry of Education, China
    J Biomed Opt 10:064012. 2005
    ..Furthermore, a dependence on particle size is found for these interactions in the different cell lines. The results suggest that after optimization, this method could be used for gene transfection and gene therapy...
  53. ncbi Liposomes increase skin penetration of entrapped and non-entrapped hydrophilic substances into human skin: a skin penetration and confocal laser scanning microscopy study
    D D Verma
    Institut für Pharmazeutische Technologie und Biopharmazie, Philipps Universitat Marburg, Marburg, Germany
    Eur J Pharm Biopharm 55:271-7. 2003
    ..The results indicated that phospholipid vesicles not only carry the entrapped hydrophilic substance, but also the non-entrapped hydrophilic substance into the SC and possibly into the deeper layers of the skin...
  54. ncbi Keratoconjunctivitis sicca modifies epithelial stem cell proliferation kinetics in conjunctiva
    Wensheng Chen
    Tianjin Medical University Eye Center, Tianjin, China
    Cornea 26:1101-6. 2007
    ..The objective of this study was to examine the epithelial stem cell proliferation kinetics in a rat model with keratoconjunctivitis sicca (KCS)...
  55. pmc Postnatal age influences hypoglycemia-induced neuronal injury in the rat brain
    Kathleen Ennis
    Division of Neonatology, Department of Pediatrics, University of Minnesota, Minneapolis, MN 55455, USA
    Brain Res 1224:119-26. 2008
    ..Varying the duration of fasting did not alter the severity of regional cell injury. These results suggest that postnatal age influences the regional vulnerability to hypoglycemia-induced neuronal death in the rat brain...
  56. ncbi Pumping of drugs by P-glycoprotein: a two-step process?
    Thomas Litman
    Bioinformatics Centre, University of Copenhagen, Universitetsparken 15, Bldg 10, DK 2100 Copenhagen, Denmark
    J Pharmacol Exp Ther 307:846-53. 2003
    ..We suggest that the vinblastine/cyclosporin A/XR9576-binding site accepts daunorubicin at the cytoplasmic face and transfers it to the verapamil-binding site, from where daunorubicin is emptied at the extracellular surface...
  57. ncbi Evaluation and comparison of MRP1 activity with three fluorescent dyes and three modulators in leukemic cell lines
    A Lale Dogan
    Laboratoire INSERM E03 55 EA 1529, Département d Onco Hématologie, Hôpital Hotel Dieu AP HP, Universite Paris 6, 1 Place du Parvis Notre Dame, Paris 75004, France
    Leuk Res 28:619-22. 2004
    ..In conclusion, calcein-AM and CFDA uptake assays are the best choices to probe MRP1 activity and combination of two modulators may improve the efficiency of these assays...
  58. ncbi Impaired renal secretion of substrates for the multidrug resistance protein 2 in mutant transport-deficient (TR-) rats
    Rosalinde Masereeuw
    Department of Pharmacology and Toxicology, University Medical Center Nijmegen, Nijmegen Center for Molecular Life Sciences, Nijmegen, The Netherlands
    J Am Soc Nephrol 14:2741-9. 2003
    ..We conclude that the renal clearance of the Mrp2 substrates calcein and fluo-3 is significantly reduced in TR(-) rat; for LY, the absence of the transporter may be compensated for by (an)other organic anion transporter(s)...
  59. ncbi Loss or mismatch of MHC class I is sufficient to trigger NK cell-mediated rejection of resting lymphocytes in vivo - role of KARAP/DAP12-dependent and -independent pathways
    Linda Oberg
    Microbiology and Tumor Biology Center, Karolinska Institutet, Stockholm, Sweden
    Eur J Immunol 34:1646-53. 2004
    ..However, activation of the effector or the target enhanced rejection and shifted the balance between different signaling pathways involved...
  60. ncbi Fluorophotometric determination of hydrogen peroxide with fluorescin in the presence of cobalt (II) and reaction against other reactive oxygen species
    Ryosuke Nakahara
    Department of Clinical Chemistry, Osaka University of Pharmaceutical Sciences, 4 20 1 Nasahara, Takatsuki, Osaka 569 1094, Japan
    J Fluoresc 19:769-75. 2009
    ..and it was possible to detect them with a high sensitivity. In addition, this proposed method was applied to the recovery tests of H2O2 in calf serum, human saliva, rain water, and wheat noodles; the results were satisfactory...
  61. ncbi Distributed parameter identification for a label-structured cell population dynamics model using CFSE histogram time-series data
    Tatyana Luzyanina
    Institute of Mathematical Problems in Biology, RAS, Pushchino, Russia
    J Math Biol 59:581-603. 2009
    ..We show that the solution of the regularized parameter estimation problem is consistent with the data set with an accuracy within the noise level in the measurements...
  62. pmc Functional detection of MDR1/P170 and MRP/P190-mediated multidrug resistance in tumour cells by flow cytometry
    N Feller
    Department of Medical Oncology, Free University Hospital, Amsterdam, The Netherlands
    Br J Cancer 72:543-9. 1995
    ..With these functional assays the role and relative importance of P-gp and MRP can be studied in, for example, haematological malignancies...
  63. ncbi Intracellular generation of reactive oxygen species by contracting skeletal muscle cells
    Francis McArdle
    Division of Metabolic and Cellular Medicine, School of Clinical Sciences, University of Liverpool, Liverpool L69 3GA, UK
    Free Radic Biol Med 39:651-7. 2005
    ....
  64. ncbi Application of biotin-4-fluorescein in homogeneous fluorescence assays for avidin, streptavidin, and biotin or biotin derivatives
    Andreas Ebner
    University of Linz, Linz, Austria
    Methods Mol Biol 418:73-88. 2008
    ..This competitive assay correctly measures the small dose of d-biotin in multivitamin tablets (e.g., 150 microg in 5 g of solid) after subtracting the background fluorescence of the colored aqueous solution...
  65. ncbi Functionality of drug efflux pumps in antimonial resistant Leishmania donovani field isolates
    Goutam Mandal
    Department of Pharmacology, Institute of Post Graduate Medical Education and Research, Kolkata, 244B Acharya JC Bose Road, Kolkata 700 020 India
    Indian J Biochem Biophys 46:86-92. 2009
    ..In conclusion, our data suggested that overfunctioning of a MRP-like pump contributed towards generation of Sb-R phenotype in L. donovani field isolates...
  66. ncbi Differential expression of sphingolipids in MRP1 overexpressing HT29 cells
    J W Kok
    Department of Physiological Chemistry, Groningen Institute of Drug Studies, University of Groningen, Groningen, The Netherlands
    Int J Cancer 87:172-8. 2000
    ..In addition, ceramide, a lipid messenger molecule involved in apoptosis-related signal transduction processes, was much more abundant in HT29(col) cells, which is indicative of a stress response...
  67. ncbi Shell click-crosslinked (SCC) nanoparticles: a new methodology for synthesis and orthogonal functionalization
    Maisie J Joralemon
    Washington University in Saint Louis, Center for Materials Innovation and Department of Chemistry, Missouri 63130 4899, USA
    J Am Chem Soc 127:16892-9. 2005
    ....
  68. ncbi The oxidation of 2',7'-dichlorofluorescin to reactive oxygen species: a self-fulfilling prophesy?
    Marcelo G Bonini
    Laboratory of Pharmacology and Chemistry, Ntional Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 40:968-75. 2006
    ..Our findings demonstrate that this assay is subject to a serious artifact in that it produces what it is purported to measure; therefore, its use in biological systems should be approached with caution...
  69. ncbi A simple spectrophotometric streptavidin-biotin binding assay utilizing biotin-4-fluorescein
    Mark J Waner
    Department of Chemistry, John Carroll University, 20700 North Park Blvd, University Heights, OH 44118 USA
    J Biochem Biophys Methods 70:873-7. 2008
    ....
  70. ncbi Statistical determination of threshold for cellular division in the CFSE-labeling assay
    Dacheng Liu
    Department of Biostatistics and Computational Biology, University of Rochester School of Medicine and Dentistry, Rochester, New York, NY 14642, USA
    J Immunol Methods 312:126-36. 2006
    ..071% (95% confidence) and 0.114% (99% confidence) of total CFSE-labeled cells under our laboratory conditions. We offer our methods presented here for other investigators to calculate a threshold in their own CFSE-labeling experiments...
  71. pmc Flow cytometric assessment of membrane integrity of ethanol-stressed Oenococcus oeni cells
    M Graça da Silveira
    Instituto de Tecnologia Quimica e Biologica, 2780 Oeiras, Portugal
    Appl Environ Microbiol 68:6087-93. 2002
    ..This information is of major importance in studies of microbial fermentations in order to assign bulk activities measured by classical methods to the very active cells that are effectively responsible for the observations...
  72. ncbi Endogenous and endobiotic induced reactive oxygen species formation by isolated hepatocytes
    Arno G Siraki
    Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Toronto, 19 Russell Street, Toronto, Ontario, Canada M5S 2S2
    Free Radic Biol Med 32:2-10. 2002
    ..Hepatocyte catalyzed dichlorofluorescin oxidation induced by oxidase substrates, e.g., benzylamine, was correlated with the cytotoxicity induced in catalase-inhibited hepatocytes...
  73. ncbi Measurement of diffusion within the cell wall in living roots of Arabidopsis thaliana
    Eric M Kramer
    Biology Department, University of Massachusetts, 611 N Pleasant St, Amherst, MA 01003, USA
    J Exp Bot 58:3005-15. 2007
    ..These measurements constrain models of solute transport, and are important for quantitative models of hormone signalling during plant growth and development...
  74. ncbi Caged probes: a novel tool in studying symplasmic transport in plant tissues
    H J Martens
    Department of Plant Biology, Royal Veterinary and Agricultural University, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark
    Protoplasma 223:63-6. 2004
    ..Transport via the symplasmic pathway was challenged by plasmolysing the tissue. The experiments confirmed the symplasmic nature of tracer transport...
  75. ncbi Quantitative tracking of Cryptosporidium infection in cell culture with CFSE
    Hanping Feng
    Division of Infectious Diseases, Tufts University, Cummings School of Veterinary Medicine, North Grafton, Massachusetts 01536, USA
    J Parasitol 92:1350-4. 2006
    ..Unlike immunofluorescent assays, CFSE labeling permitted the tracking of the initial invasion of C. parvum. Such an assay may be useful for studying the dynamics of host cell-parasite interaction and possibly for drug screening...
  76. ncbi Heterogeneous cell response to topotecan in a CFSE-based proliferation test
    Giada Matera
    Biophysics Unit, Department of Oncology, Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy
    Cytometry A 62:118-28. 2004
    ..However, intercell variability of CFSE loading results in overlapping peaks, thereby limiting its use with cancer cell lines...
  77. ncbi Self-assembly of recombinant amphiphilic oligopeptides into vesicles
    Albert J van Hell
    Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, P O BOX 80082, 3508 TB Utrecht, The Netherlands
    Biomacromolecules 8:2753-61. 2007
    ..In addition, it was demonstrated that water-soluble molecules can be entrapped inside these peptide vesicles. Such peptide vesicles may find applications as biodegradable drug delivery systems with a pH-dependent release profile...
  78. ncbi Computational analysis of CFSE proliferation assay
    Tatyana Luzyanina
    Institute of Mathematical Problems in Biology, RAS, Pushchino, Russia
    J Math Biol 54:57-89. 2007
    ..Overall, our study suggests that the heterogeneity inherent in cell kinetics should be explicitly incorporated into the structure of mathematical models...
  79. pmc Use of CFSE staining of borreliae in studies on the interaction between borreliae and human neutrophils
    Helena Tuominen-Gustafsson
    Department of Medical Microbiology, University of Turku, Kiinamyllynkatu 13, FI 20520 Turku, Finland
    BMC Microbiol 6:92. 2006
    ..Carboxyfluorescein diacetate, succinimidyl ester (CFSE) labelling has been previously used for studying the adhesion of labelled bacteria to host cells and the uptake of labelled substrates by various cells using flow cytometry...
  80. pmc Eosin B as a novel antimalarial agent for drug-resistant Plasmodium falciparum
    Kristen M Massimine
    Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06520, USA
    Antimicrob Agents Chemother 50:3132-41. 2006
    ..In addition, preliminary studies suggest that eosin B is also acting as a redox cycling compound. Overall, our data suggest that eosin B is an effective lead compound for the development of new, more effective antimalarial drugs...
  81. ncbi Estimating lymphocyte division and death rates from CFSE data
    Rob J de Boer
    Theoretical Biology, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands
    Bull Math Biol 68:1011-31. 2006
    ..J. Immunol. 170:4963-4972, 2003] model into the classical smith-martin model, and into a model with arbitrary probability distributions for death and division through subsequent divisions...
  82. pmc Quantifying lymphocyte kinetics in vivo using carboxyfluorescein diacetate succinimidyl ester (CFSE)
    Becca Asquith
    Department of Immunology, Imperial College London, Norfolk Place, London W2 1PG, UK
    Proc Biol Sci 273:1165-71. 2006
    ..We illustrate this approach using in vivo CFSE labelling of B lymphocytes in sheep...
  83. ncbi pH fluorescent probes: chlorinated fluoresceins
    Feng Yan Ge
    College of Chemistry and Chemical Engineering, Donghua University, Shanghai, People s Republic of China
    J Fluoresc 18:741-7. 2008
    A series of regiospecific chlorinated fluoresceins have been synthesized by the reaction of the regiospecific chlorinated resorcinols with chlorinated phthalic anhydride. The regioisomers were successfully separated by chromatography...
  84. pmc Reconstruction of cell population dynamics using CFSE
    Andrew Yates
    Department of Biology, Emory University, Atlanta, GA 30322, USA
    BMC Bioinformatics 8:196. 2007
    ..This gives rise to heterogeneity in proliferating cell populations. Branching processes provide a natural means of describing this behaviour...
  85. ncbi Endothelin B receptor-mediated regulation of ATP-driven drug secretion in renal proximal tubule
    R Masereeuw
    Department of Pharmacology, University of Nijmegen, Nijmegen, The Netherlands
    Mol Pharmacol 57:59-67. 2000
    ..These are the first results linking ET to the control of xenobiotic transport and the first demonstrating control of renal multidrug resistance-associated protein 2 and P-glycoprotein by a hormone...
  86. ncbi A comparative study of carboxyfluorescein diacetate and carboxyfluorescein diacetate succinimidyl ester as indicators of bacterial activity
    Daniel Hoefel
    The Cooperative Research Centre for Water Quality and Treatment, Australian Water Quality Centre, SA Water Corporation, Salisbury, South Australia 5108, Australia
    J Microbiol Methods 52:379-88. 2003
    ..This study shows that CFDA/SE is a poor marker of bacterial activity...
  87. ncbi Divided we stand: tracking cell proliferation with carboxyfluorescein diacetate succinimidyl ester
    A B Lyons
    Discipline of Pathology, The University of Tasmania, Hobart, Tasmania, Australia
    Immunol Cell Biol 77:509-15. 1999
    ..Furthermore, viable cells from defined generation numbers can be sorted by flow cytometry for functional analysis...
  88. ncbi A novel flow cytometric assay for quantitating adherence of Helicobacter pylori to gastric epithelial cells
    R P Logan
    Division of Gastroenterology, University Hospital, Nottingham, UK
    J Immunol Methods 213:19-30. 1998
    ..Fluorescent labelling of H. pylori with CFDA-SE in combination with flow cytometry provides a quick, specific, and sensitive method to quantitate in vitro the adherence of H. pylori...
  89. ncbi Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution
    A B Lyons
    Discipline of Pathology, Faculty of Health Science, The University of Tasmania, GPO Box 252 29, 7001, Hobart, TAS, Australia
    J Immunol Methods 243:147-54. 2000
    ..The CFSE technique can be used to determine kinetics of immune responses, track proliferation in minor subsets of cells and follow the acquisition of differentiation markers or internal proteins linked to cell division...
  90. ncbi Fluorescent dyes for lymphocyte migration and proliferation studies
    C R Parish
    Division of Immunology and Cell Biology, John Curtin School of Medical Research, Australian National University, Canberra, Australian Capital Territory, Australia
    Immunol Cell Biol 77:499-508. 1999
    ..In the future it is highly likely that additional fluorescent dyes, with different spectral properties to CFSE, will become available, as well as membrane inserting fluorescent dyes that more homogeneously label lymphocytes than PKH26...
  91. pmc Noninvasive in vivo fluorescence measurement of airway-surface liquid depth, salt concentration, and pH
    S Jayaraman
    Departments of Medicine and Physiology, Cardiovascular Research Institute, University of California, San Francisco, California 94143-0521, USA
    J Clin Invest 107:317-24. 2001
    ..78 +/- 0.2. These results establish by a noninvasive approach the key properties of the ASL and provide direct evidence that the ASL is approximately isotonic and not saltier in cystic fibrosis...
  92. ncbi beta-lactamase as a marker for gene expression in live zebrafish embryos
    E Raz
    Institute for Biology 1, University of Freiburg, Hauptstrasse 1, Freiburg, D 79104, Germany
    Dev Biol 203:290-4. 1998
    ..We suggest that this new reporter system provides a major advancement in sensitivity over the existing methods for monitoring gene expression in vivo during early embryogenesis...
  93. ncbi A molecular docking strategy identifies Eosin B as a non-active site inhibitor of protozoal bifunctional thymidylate synthase-dihydrofolate reductase
    Chloe E Atreya
    Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 278:14092-100. 2003
    ..Additionally, eosin B was found to be a 180 microm inhibitor of Toxoplasma gondii in both biochemical and cell culture assays...
  94. ncbi Fluorescent sensors for Zn(2+) based on a fluorescein platform: synthesis, properties and intracellular distribution
    S C Burdette
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA
    J Am Chem Soc 123:7831-41. 2001
    ..The X-ray crystal structure of a 2:1 Zn(2+):Zinpyr-1 complex has also been solved, and is the first structurally characterized example of a complex of fluorescein substituted with metal binding ligands...
  95. ncbi Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader
    H Wang
    Neuroscience Laboratory, USDA ARS, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA 02111, USA
    Free Radic Biol Med 27:612-6. 1999
    ..This method is reliable and efficient for evaluating the potency of pro-oxidants and can be used to evaluate the efficacy of antioxidants against OS in cells...
  96. ncbi Comparison of in vitro P-glycoprotein screening assays: recommendations for their use in drug discovery
    Dietmar Schwab
    F Hoffmann LaRoche Ltd, Pharmaceuticals Division, CH 4070 Basel, Switzerland
    J Med Chem 46:1716-25. 2003
    ..Recommendations are given on screening strategies which can be applied to different phases of the drug discovery and development process...
  97. pmc Analysis of cell kinetics using a cell division marker: mathematical modeling of experimental data
    Samuel Bernard
    Département de mathématiques et de statistique and Centre de recherches mathématiques, Universite de Montreal, Montreal, Quebec H3C 3J7, Canada
    Biophys J 84:3414-24. 2003
    ..Our results indicate that after three days in vitro, primitive murine bone marrow cells have parameters beta = 2.2 day(-1), tau = 0.3 day, gamma = 0.3 day(-1), and micro = 0.05 day(-1)...
  98. ncbi Design of a practical fluorescent probe for superoxide based on protection-deprotection chemistry of fluoresceins with benzenesulfonyl protecting groups
    Hatsuo Maeda
    Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamada oka, Suita, Osaka 565 0871, Japan
    Chemistry 13:1946-54. 2007
    A strategy for designing probes based on protection-deprotection chemistry involving fluoresceins and their benzenesulfonyl (BES) derivatives has led to the development of a much more practical superoxide (O(2) (-...
  99. ncbi Polyamine deficiency leads to accumulation of reactive oxygen species in a spe2Delta mutant of Saccharomyces cerevisiae
    Manas K Chattopadhyay
    Laboratory of Biochemistry and Genetics, Building 8, Room 223, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Yeast 23:751-61. 2006
    ..Polyamine-deficient cells incubated anaerobically show markedly less cell death. These data show that part of the function of polyamines is protection of the cells from accumulation of ROS...
  100. ncbi Regulation of reactive oxygen species, DNA damage, and c-Myc function by peroxiredoxin 1
    Rachel A Egler
    Department of Pediatrics, Section of Hematology Oncology, Children s Hospital of Pittsburgh, Rangos Research Center, 3460 Fifth Ave, USA
    Oncogene 24:8038-50. 2005
    ..prdx1-/- mice should be useful in studying the role of oxidative DNA damage in the causation of cancer and its prevention by antioxidants. They should also help in studying the relationship between oncogenes such as c-Myc and DNA damage...
  101. ncbi A novel flow cytometric assay for quantitation and multiparametric characterization of cell-mediated cytotoxicity
    H Lecoeur
    , URA CNRS 1930, , Institut Pasteur, 28 rue du Dr. Roux, 75724 Cedex 15, Paris, France
    J Immunol Methods 253:177-87. 2001
    ..This new assay should contribute to a better understanding of the mechanisms involved in cell-mediated cytotoxicity in normal and pathological situations...

Research Grants69

  1. Novel Extended Red-Shifted Rhodamine Dyes
    NICHOLAS CAIRNS; Fiscal Year: 2004
    ..In existing applications of commonly used short and medium wavelength dyes such as coumarins, fluoresceins and rhodamines, assay or tissue components can severely interfere with the fluorescence properties of tracers ..
  2. DEVELOPMENT OF CHLORIDE-SENSITIVE FLUORESCENT INDICATORS
    Alan Verkman; Fiscal Year: 1991
    ..Based on the utility of fluorescence probes in examining physiological questions (fluoresceins to study pH, fura-2 to study Ca), we have been developing new fluorescence methods to study Cl transport in ..
  3. Modeling, Estimation and Control in HIV Dynamics
    H Banks; Fiscal Year: 2005
    ....
  4. In Vivo Gene Silencing with siRNA After Brain Trauma
    HELEN HELLMICH; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  5. Mineral and Mechanical Properties of Bone
    Sarandeep Huja; Fiscal Year: 2005
    ..Future interventional studies will examine the mechanisms for the initial and the long-term adaptive response in material properties of bone. ..
  6. GENE EXPRESSION-ENVIRONMENT LINK IN CEREBRAL ISCHEMIA
    TERESITA BRIONES; Fiscal Year: 2006
    ..Additionally, the sensory motor deficit seen following ischemic damage can affect the long-term outcome of these survivors. ..
  7. A THERAPEUTIC MODEL FOR CEREBRAL ISCHEMIA
    TERESITA BRIONES; Fiscal Year: 2003
    ..From a nursing perspective, results from this study are important clinically because the needs of most survivors of brain injury often times are for continuous nursing, while use of medical services may be intermittent. ..
  8. Antioxidant Functions of Lipoic Acid
    James May; Fiscal Year: 2004
    ..Despite the widespread use of lipoic acid, these studies are necessary to establish the biological basis and rationale for the clinical use of the agent. ..
  9. MOLECULAR ANGLE TRANSITIONS IN MUSCLE
    THOMAS BURGHARDT; Fiscal Year: 2001
    ....
  10. Antioxidant vitamins in models of Alzheimer's Disease
    James May; Fiscal Year: 2008
    ..If antioxidant vitamins ameliorate toxicity in cell and animal models of Alzheimer's disease, then oxidant stress is involved in disease progression, and antioxidant vitamin supplements may be beneficial. ..
  11. Antioxidant Interaction of Selenium and Vitamins C & E
    James May; Fiscal Year: 2008
    ..Using this approach, it will be possible to relate the crucial, mitochondrial functions of selenium and vitamins C and E to the antioxidant defenses of the whole animal. ..
  12. AGE/NUTRITION/GENES IN MODELS OF PSYCHIATRIC DISORDERS
    Gary Gibson; Fiscal Year: 2007
    ..An understanding of these mechanisms will likely suggest new ways to overcome the consequences of the mild, chronic impairment of oxidative metabolism that accompanies numerous age-related neurodegenerative disorders. ..
  13. Aging as a risk factor for seizure-induced cell death
    PAULA SCHAUWECKER; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  14. Multiphoton microscopy using near infrared Dyes
    Samuel Achilefu; Fiscal Year: 2006
    ..Translation of the method and instrument developed from small animals to imaging of human Gl and cervical cancers is expected at the completion of the study. ..
  15. Synchrotron-Based Infrared Microscope for Chemical Imaging of Disease
    Lisa Miller; Fiscal Year: 2007
    ..This instrument will be unique worldwide. [unreadable] [unreadable] [unreadable]..
  16. Metal-Enhanced Fluorescence Sensing
    Joseph Lakowicz; Fiscal Year: 2006
    ..b. Develop RET immunoassays for PSA (or ACT-PSA) with the capture and detection antibodies. [unreadable] [unreadable]..
  17. A Chaperonin/Osmolyte Protein Folding Screen - STTR Phase I
    Mark Fisher; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  18. Peroxide, NO and Iron Signaling in Endothelial Damage
    Balaraman Kalyanaraman; Fiscal Year: 2007
    ..Novelty: This proposal presents a new insight on peroxide-induced iron signaling in endothelial apoptosis and on the role of NO and antioxidants in mitigating these effects. ..
  19. Metal Carcinogenesis: New Concepts
    Anatoly Zhitkovich; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  20. MICELLAR CARRIERS FOR SPARINGLY SOLUBLE PHARMACEUTICALS
    Vladimir Torchilin; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  21. ADVANCES IN OPTICS FOR BIOTECHNOLOGY, MEDICINE AND SURGERY CONFERENCE
    Samuel Achilefu; Fiscal Year: 2007
    ..unreadable] Aim 4: To promote interaction between the invited scientists and participating students, and to enable [unreadable] collaborations. [unreadable] [unreadable] [unreadable]..
  22. MULTIMODAL DOT-PET MOLECULAR PROBES AND TANDEM SYSTEM
    Joseph P Culver; Fiscal Year: 2010
    ..abstract_text> ..
  23. Polypeptide Conformation and Interaction with Hsp70
    Silvia Cavagnero; Fiscal Year: 2009
    ..abstract_text> ..
  24. Elevated potassium ion in lacrimal fluid and the health of the ocular surface
    John Ubels; Fiscal Year: 2009
    ..If the high potassium in tears is important for the health of the ocular surface, this information will be useful in the development of improved treatments for dry eye disease. ..
  25. PHYSICOCHEMICAL INVESTIGATION OF TASTE
    Vijay Lyall; Fiscal Year: 2010
    ..The research will reveal how the sour taste defense mechanism functions to prevent the ingestion of acids. ..
  26. Chemical Approaches for the Discovery of New Cancer Therapeutic Targets
    Kazunori Koide; Fiscal Year: 2009
    ....
  27. Long-Circulating Polymer-Modified Liposomes
    Vladimir Torchilin; Fiscal Year: 2008
    ..abstract_text> ..
  28. Sensitivity Mechanisms in Chromium Toxicity
    Anatoly Zhitkovich; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  29. Genetics of neuromuscular synaptogenesis in zebrafish
    Rita Balice Gordon; Fiscal Year: 2008
    ..Identification of the underlying genetic defects in these mutants will expand our understanding of the molecular mechanisms that mediate the formation and function of neuromuscular and other synapses. ..
  30. Novel Neuroprotectants for TBI
    Edward Hall; Fiscal Year: 2008
    ..Thousands experience long lasting brain injury that interferes with their ability to lead productive and full lives. We plan to test a promising new class of therapeutics for traumatic brain injury. [unreadable] [unreadable] [unreadable]..
  31. Renal medullary COX-2 in blood pressure control
    Tianxin Yang; Fiscal Year: 2008
    ..The information resulted from these studies will provide new insight into the molecular mechanism responsible for long-term control of arterial blood pressure. ..
  32. Interaction of COX-2 AND nNOS in renin secretion (R01)
    Tianxin Yang; Fiscal Year: 2008
    ..abstract_text> ..
  33. Image-guided Ultrasound-triggered Tumor Therapy
    Alexander Klibanov; Fiscal Year: 2007
    ..Monitor blood flow within the tumor after treatment. Perform final optimization of the drug carrier formulation and ultrasound activation parameters. ..
  34. Chimeric Proteins to Enhance Crystal Formation of G Protein Coupled Receptors
    Brian Kobilka; Fiscal Year: 2006
    ..Drugs acting on GPCRs can have an impact on a broad spectrum of diseases including: cardiovascular disease, pulmonary disease, inflammation, diabetes and obesity, behavioral disorders and Alzheimer's disease. ..
  35. HLA-DQ-derived RTLs for Treatment of Celiac Disease
    GREGORY BURROWS; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  36. ENOS & THE RADICAL MECHANISM OF ANTITUMOR ANTHRACYCLINES
    Balaraman Kalyanaraman; Fiscal Year: 2006
    ..Understanding the relationship between DOX-induced apoptosis and ROS formation may help discover novel antioxidant/antiapoptotic therapy in oxidant-induced disease processes. ..
  37. Effects of Antioxidants on Human Macular Pigments
    Kyung Jin Yeum; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  38. CALCIUM DEPENDENT MEMBRANE BINDING PROTEINS
    CARL CREUTZ; Fiscal Year: 2002
    ....
  39. HYPOGLYCEMIA-ASSOCIATED AUTONOMIC FAILURE AND THE BRAIN
    Nancy Tkacs; Fiscal Year: 2003
    ..The research plan, candidate, and environment are well-suited for an ongoing career in fundamental diabetes research. ..
  40. SIGNAL TRANSDUCTION SYSTEMS IN ALZHEIMERS DISEASE
    Gary Gibson; Fiscal Year: 2003
    ..abstract_text> ..
  41. THE ROLE OF UROKINASE IN LUNG T LYMPHOCYTE RESPONSES
    MARGARET GYETKO; Fiscal Year: 2003
    ..Determine if recombinant uPA can reverse the functional abnormalities in uPA minus/minus lymphocytes. ..
  42. K + EFFLUX--ROLE IN CA++ HOMEOSTASIS AND EXCITOTOXICITY
    Lech Kiedrowski; Fiscal Year: 2003
    ..These studies may yield a new target for pharmacological intervention to decrease neuronal death following ischemia or hypoglycemia, namely, to prevent excessive K+ loss from the brain during reperfusion. ..
  43. SPECIFICATION OF FAST AND SLOW MUSCLES IN ZEBRAFISH
    Shao Jun Du; Fiscal Year: 2003
    ..The long-term goal of this study is to elucidate the molecular mechanisms behind determination of muscle-specific cell fate during embryonic development. ..
  44. MECHANISM FOR BONE SIALOPROTEIN TO NUCLEATE BIOAPATITE
    RONALD MIDURA; Fiscal Year: 2002
    ..abstract_text> ..
  45. Dietary Cysteine:Conceptal Response to Oxidative Stress
    Craig Harris; Fiscal Year: 2002
    ....
  46. ROLE OF NITRIC OXIDE IN FERTILIZATION
    David Epel; Fiscal Year: 2002
    ..The possible role of NO in regulating the egg's ion channels (as part of polyspermy block) and sperm physiology (such as motility and the acrosome reaction) will also be investigated. ..
  47. FATTY ACID METABOLISM IN CARDIAC ISCHEMIA
    DIANE HICKSON BICK; Fiscal Year: 2002
    ..Location of a malonyl-CoA compartment that is inaccessible to CPT-I will shed light on the inconsistency between the high malonyl CoA content in heart and reliance on beta-oxidation. ..
  48. A HIGH PRESSURE FREEZER FOR ELECTRON MICROSCOPY USES
    GINA SOSINSKY; Fiscal Year: 2002
    ..In all of these research projects, the primary goal is to understand the three-dimensional structure of organized cellular components and relate this morphology to their functionality...
  49. A Freeze-Fracture & Etching System for Northwestern Univ
    Mark Johnson; Fiscal Year: 2002
    ..abstract_text> ..
  50. NF-KB SIGNAL TRANSDUCTION IN BRAIN INJURY
    KEITH PENNYPACKER; Fiscal Year: 2003
    ..These studies will define the role of NF-kB in the process of brain injury and subsequent repair and regeneration processes. ..
  51. Glial Maintenance of Local K+ and Neurotransmission
    JULIAN MEEKS; Fiscal Year: 2005
    ..These studies will determine the functional relevance of KIRs in hippocampal astrocytes and provide new insight into the role of KIRs in passive [K+]o buffering. ..
  52. CENTER FOR FLUORESCENCE SPECTROSCOPY
    Joseph Lakowicz; Fiscal Year: 2006
    ..Abstract Not Provided. ..
  53. Genetic approaches to neurotrophin signaling
    Rita Balice Gordon; Fiscal Year: 2006
    ....
  54. Biophysical Analysis of Opioid Receptor Structure
    Brian Kobilka; Fiscal Year: 2005
    ..Successful completion of these aims will provide the preliminary data for a more comprehensive study of opioid receptor structure and conformational dynamics in a Stage II CEBRA project. ..
  55. Peroxynitrite-Induced Oxidative Damage in TBI
    Edward Hall; Fiscal Year: 2007
    ..abstract_text> ..
  56. Symposium on Imaging Agents and Molecular Beacons
    Samuel Achilefu; Fiscal Year: 2004
    ..Five sessions will be held and will cover topics on nuclear, magnetic resonance, ultrasound, radiographic, optical and miscellaneous imaging agents for medical applications. ..
  57. ANTIBODY-TARGETED POLYMERIC SYSTEMS FOR TUMOR IMAGING
    Vladimir Torchilin; Fiscal Year: 2006
    ..abstract_text> ..
  58. Meeting:Society for Integrative and Comparative Biology
    Neil Blackstone; Fiscal Year: 2004
    ..Ultimately, this work will allow the power of genomics to be fully realized in biology and medicine. ..
  59. ANNEXIN PHOSPHORYLATION: CAUSES AND CONSEQUENCES
    CARL CREUTZ; Fiscal Year: 2004
    ....
  60. MOLECULAR BIOLOGY OF INTERCELLULAR JUNCTIONS
    Daniel Goodenough; Fiscal Year: 2006
    ..If these connexins are expressed in retinal neurons, mouse lines with targeted gene knockouts of these connexins will be prepared and studied for functional consequences. ..
  61. ROLE OF SPHINGOMYELIN IN LIPOPROTEIN METABOLISM
    Papasani Subbaiah; Fiscal Year: 2008
    ..These studies will provide novel insights into the physiological role of this important phospholipid, and could possibly open up novel therapeutic targets against inflammation and atherosclerosis. ..
  62. Comparative In Vivo Biodistribution of Characterized Manufactured Nanomaterials
    Nancy A Monteiro Riviere; Fiscal Year: 2010
    ..These coordinated studies will provide data to link physical chemical characterizations to in vivo MNM disposition. ..
  63. LCAT AND PATHOPHYSIOLOGY OF PHOSPHOLIPID SPECIES
    Papasani Subbaiah; Fiscal Year: 2002
    ..These studies should provide novel insights into the physiological importance of LCAT beyond its role in cholesterol esterification. ..
  64. BIACORE 3000 Biosensor
    Robert Bloch; Fiscal Year: 2002
    ..We anticipate that this facility will provide its primary users, and ultimately others at the SoM and in the Baltimore area, with state-of-the-art biosensor technology. ..
  65. A Shared Multiparameter Imaging Cytometer
    Paul Wallace; Fiscal Year: 2006
    ..All the projects described in this application are relevant to the treatment of cancer and obtaining a better understanding of its biology. [unreadable] [unreadable] [unreadable]..
  66. INDICES OF BIOTIN NUTRITION
    Donald Mock; Fiscal Year: 2001
    ..4) Whether biotin deficiency of similar severity to that observed either early or late in human gestation causes increased rates of fetal malformation in the mouse. 5) The enzyme(s) responsible for biotin beta-oxidation. ..
  67. The Sarcolemma in FSHD and in the myd Mouse
    Robert Bloch; Fiscal Year: 2003
    ..We therefore anticipate making significant progress in understanding the cell biological changes that occur in FSHD skeletal muscle, and in determining which, if any, of these changes are related to the pathophysiology of FSHD. ..
  68. Obscurin & Myofibrils in Cardiac & Skeletal Muscle
    Robert Bloch; Fiscal Year: 2009
    ..The results of our experiments should reveal some of the key mechanisms involved in assembling contractile proteins into sarcomeres and in determining their association with the SR. ..