xanthenes

Summary

Summary: Compounds with three aromatic rings in linear arrangement with an OXYGEN in the center ring.

Top Publications

  1. pmc Multiple applications of Alamar Blue as an indicator of metabolic function and cellular health in cell viability bioassays
    Sephra N Rampersad
    Department of Life Sciences, The University of the West Indies, West Indies, St Augustine, Trinidad and Tobago
    Sensors (Basel) 12:12347-60. 2012
  2. ncbi A new approach to drug discovery: high-throughput screening of microbial natural extracts against Aspergillus fumigatus using resazurin
    Maria Cândida Monteiro
    Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Armilla, Granada, Spain
    J Biomol Screen 17:542-9. 2012
  3. ncbi Antimycobacterial screening of traditional medicinal plants using the microplate resazurin assay
    Duncan Webster
    Department of Medicine, Saint John Regional Hospital, Canada
    Can J Microbiol 56:487-94. 2010
  4. ncbi Comparison of alamar blue and MTT assays for high through-put screening
    R Hamid
    Discovery Support, Purdue Pharma L P, 444 Saw Mill River Road, Ardsley, NY 10502, USA
    Toxicol In Vitro 18:703-10. 2004
  5. ncbi Detection of mitochondrial respiratory dysfunction in circulating lymphocytes using resazurin
    Khaled K Abu-Amero
    Department of Genetics, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia
    Arch Pathol Lab Med 129:1295-8. 2005
  6. ncbi Pharmacological blockade of mGlu2/3 metabotropic glutamate receptors reduces cell proliferation in cultured human glioma cells
    Mara D'Onofrio
    INM Neuromed, Pozzilli, Italy
    J Neurochem 84:1288-95. 2003
  7. ncbi Resazurin reduction assays for screening of anti-tubercular compounds against dormant and actively growing Mycobacterium tuberculosis, Mycobacterium bovis BCG and Mycobacterium smegmatis
    Neetu Kumra Taneja
    Department of Biotechnology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India
    J Antimicrob Chemother 60:288-93. 2007
  8. ncbi Pharmacological manipulation of mGlu2 receptors influences cognitive performance in the rodent
    Guy A Higgins
    Preclinical CNS Research, F Hoffmann La Roche Ltd, PRBD N, 72 150, Grenzacherstrasse, CH 4070 Basel, Switzerland
    Neuropharmacology 46:907-17. 2004
  9. ncbi Prostaglandin E2 activates channel-mediated calcium entry in human erythrocytes: an indication for a blood clot formation supporting process
    Lars Kaestner
    Institute for Molecular Cell Biology, Faculty of Medicine, Building 61, Saarland University, 66421 Homburg Saar, Germany
    Thromb Haemost 92:1269-72. 2004
  10. ncbi An improved resazurin-based cytotoxicity assay for hepatic cells
    M K McMillian
    Department of Molecular Toxicology, The R W Johnson Pharmaceutical Research Institute, Raritan, NJ 08869, USA
    Cell Biol Toxicol 18:157-73. 2002

Research Grants

Detail Information

Publications236 found, 100 shown here

  1. pmc Multiple applications of Alamar Blue as an indicator of metabolic function and cellular health in cell viability bioassays
    Sephra N Rampersad
    Department of Life Sciences, The University of the West Indies, West Indies, St Augustine, Trinidad and Tobago
    Sensors (Basel) 12:12347-60. 2012
    ..This review seeks to highlight many of the important considerations involved in assay use and design in addition to the potential pitfalls...
  2. ncbi A new approach to drug discovery: high-throughput screening of microbial natural extracts against Aspergillus fumigatus using resazurin
    Maria Cândida Monteiro
    Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Armilla, Granada, Spain
    J Biomol Screen 17:542-9. 2012
    ..Our group has been the first to use this methodology for screening a collection of natural extracts to identify compounds with antifungal activity against the medically important human pathogen A. fumigatus...
  3. ncbi Antimycobacterial screening of traditional medicinal plants using the microplate resazurin assay
    Duncan Webster
    Department of Medicine, Saint John Regional Hospital, Canada
    Can J Microbiol 56:487-94. 2010
    ..maximum and Acorus calamus possess strong in vitro antimycobacterial activity, validates traditional knowledge, and provides potential for the development of urgently needed novel antituberculous therapeutics...
  4. ncbi Comparison of alamar blue and MTT assays for high through-put screening
    R Hamid
    Discovery Support, Purdue Pharma L P, 444 Saw Mill River Road, Ardsley, NY 10502, USA
    Toxicol In Vitro 18:703-10. 2004
    ....
  5. ncbi Detection of mitochondrial respiratory dysfunction in circulating lymphocytes using resazurin
    Khaled K Abu-Amero
    Department of Genetics, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia
    Arch Pathol Lab Med 129:1295-8. 2005
    ....
  6. ncbi Pharmacological blockade of mGlu2/3 metabotropic glutamate receptors reduces cell proliferation in cultured human glioma cells
    Mara D'Onofrio
    INM Neuromed, Pozzilli, Italy
    J Neurochem 84:1288-95. 2003
    ..We conclude that activation of group-II mGlu receptors supports the growth of human glioma cells in culture and that antagonists of these receptors should be tested for their ability to reduce tumour growth in vivo...
  7. ncbi Resazurin reduction assays for screening of anti-tubercular compounds against dormant and actively growing Mycobacterium tuberculosis, Mycobacterium bovis BCG and Mycobacterium smegmatis
    Neetu Kumra Taneja
    Department of Biotechnology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India
    J Antimicrob Chemother 60:288-93. 2007
    ..To develop simple, rapid, low-cost and robust assays for screening drugs against dormant and actively growing mycobacteria...
  8. ncbi Pharmacological manipulation of mGlu2 receptors influences cognitive performance in the rodent
    Guy A Higgins
    Preclinical CNS Research, F Hoffmann La Roche Ltd, PRBD N, 72 150, Grenzacherstrasse, CH 4070 Basel, Switzerland
    Neuropharmacology 46:907-17. 2004
    ..Conversely, mGlu2 receptor antagonists may improve certain aspects of cognition and thus represent a novel approach for the symptomatic treatment of AD...
  9. ncbi Prostaglandin E2 activates channel-mediated calcium entry in human erythrocytes: an indication for a blood clot formation supporting process
    Lars Kaestner
    Institute for Molecular Cell Biology, Faculty of Medicine, Building 61, Saarland University, 66421 Homburg Saar, Germany
    Thromb Haemost 92:1269-72. 2004
    ..Therefore, we conclude that the PGE(2) responses of red blood cells described here reveal a direct and active participation of erythrocytes in blood clot formation...
  10. ncbi An improved resazurin-based cytotoxicity assay for hepatic cells
    M K McMillian
    Department of Molecular Toxicology, The R W Johnson Pharmaceutical Research Institute, Raritan, NJ 08869, USA
    Cell Biol Toxicol 18:157-73. 2002
    ....
  11. ncbi Induction of intratumoral tumor necrosis factor (TNF) synthesis and hemorrhagic necrosis by 5,6-dimethylxanthenone-4-acetic acid (DMXAA) in TNF knockout mice
    L M Ching
    Auckland Cancer Society Research Centre, University of Auckland, New Zealand
    Cancer Res 59:3304-7. 1999
    ..TNF protein was undetectable in liver and spleen tissue from TNF knockout mice, but was evident in tissue from TNF-positive mice. These results confirm that DMXAA has the novel ability of inducing tumors to synthesize TNF in situ...
  12. ncbi Comparative effects of combretastatin A-4 disodium phosphate and 5,6-dimethylxanthenone-4-acetic acid on blood perfusion in a murine tumour and normal tissues
    R Murata
    Danish Cancer Society, Department of Experimental Clinical Oncology, Aarhus University Hospital
    Int J Radiat Biol 77:195-204. 2001
    ..To compare the ability of combretastatin A-4 disodium phosphate (CA4DP) and 5,6-dimethylxanthenone-4-acetic acid (DMXAA) to change tissue blood perfusion...
  13. pmc Pharmacological blockade of group II metabotropic glutamate receptors reduces the growth of glioma cells in vivo
    Antonietta Arcella
    I N M Neuromed, Localita Camerelle, 86077 Pozzilli, Italy
    Neuro Oncol 7:236-45. 2005
    ..These data suggest that mGlu2/3 receptor antagonists are of potential use in the experimental treatment of malignant gliomas...
  14. pmc The antitumour activity of 5,6-dimethylxanthenone-4-acetic acid (DMXAA) in TNF receptor-1 knockout mice
    L Zhao
    Auckland Cancer Society Research Centre, Faculty of Medical and Health Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand
    Br J Cancer 87:465-70. 2002
    ....
  15. pmc Activity correlation imaging: visualizing function and structure of neuronal populations
    Stephan Junek
    Department of Neurophysiology and Cellular Biophysics, University of Gottingen, Gottingen, Germany
    Biophys J 96:3801-9. 2009
    ..This method, yielding both functional and structural information of neuronal populations, will open up unprecedented possibilities for the investigation of neuronal networks...
  16. pmc Functional hair cell mechanotransducer channels are required for aminoglycoside ototoxicity
    Abdelrahman Alharazneh
    Department of Otolaryngology Head and Neck Surgery, Stanford University, Stanford, California, United States of America
    PLoS ONE 6:e22347. 2011
    ..Results suggest that limiting permeation of AGs through MET channel or preventing their entry into endolymph are potential therapeutic targets for preventing hair cell death and hearing loss...
  17. pmc TRPV4 enhances the cellular uptake of aminoglycoside antibiotics
    Takatoshi Karasawa
    Oregon Hearing Research Center, Oregon Health and Science University, 3181 Sam Jackson Park Road, Portland, OR 97239, USA
    J Cell Sci 121:2871-9. 2008
    ..In an endolymph-like extracellular environment, clearance of GTTR was attenuated from KPT2-TRPV4 cells in a TRPV4-dependent fashion. We propose that TRPV4 has a role in aminoglycoside uptake and retention in the cochlea...
  18. ncbi Sulfhydryl oxidation reduces hippocampal susceptibility to hypoxia-induced spreading depression by activating BK channels
    Sebastian Hepp
    Zentrum für Physiologie und Pathophysiologie, Abteilung Neuro und Sinnesphysiologie, Georg August Universitat Gottingen, Gottingen, Germany
    J Neurophysiol 94:1091-103. 2005
    ..Accordingly, ionic disregulation and the loss of membrane potential occur later or might even be prevented during short-term insults. Therefore well-directed oxidation of SH groups could mediate neuroprotection...
  19. ncbi Discrepancies between metabolic activity and DNA content as tool to assess cell proliferation in cancer research
    Verena M C Quent
    Regenerative Medicine, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia
    J Cell Mol Med 14:1003-13. 2010
    ..Our results show certain discrepancies in terms of over-estimation of cell proliferation with respect to the metabolic assay in comparison to DNA binding fluorophores...
  20. ncbi Development of an Alamar Blue viability assay in 384-well format for high throughput whole cell screening of Trypanosoma brucei brucei bloodstream form strain 427
    Melissa L Sykes
    Eskitis Institute for Cell and Molecular Therapies, Griffith University, Nathan, Queensland, Australia
    Am J Trop Med Hyg 81:665-74. 2009
    ..42%. Drug sensitivity was compared with those reported from previously developed 96-well methods and was found to be comparable. The sensitivity and cost benefit of the Alamar Blue assay make it an excellent candidate for HTS application...
  21. ncbi Calcium imaging of individual erythrocytes: problems and approaches
    Lars Kaestner
    Institute for Molecular Cell Biology, Faculty of Medicine, Saarland University, 66421 Homburg Saar, Germany
    Cell Calcium 39:13-9. 2006
    ..Our results emphasize that in erythrocytes measurements of intracellular calcium are reliably possible with Fluo-4 and that other indicators, especially those requiring UV-excitation, appear less favourable...
  22. ncbi A quantitative resazurin assay to determinate the viability of Trichomonas vaginalis and the cytotoxicity of organic solvents and surfactant agents
    Mariana Duarte
    Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
    Exp Parasitol 123:195-8. 2009
    ..These results contribute for pharmacological investigations of bioactive compounds that need the use of solvents as solubilization vehicles to test anti-Trichomonas activity...
  23. pmc Effects of metabotropic glutamate receptor 2/3 agonism and antagonism on schizophrenia-like cognitive deficits induced by phencyclidine in rats
    Nurith Amitai
    Department of Psychiatry, School of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Eur J Pharmacol 639:67-80. 2010
    ..Our findings also suggest that additional mechanisms, besides cortical glutamatergic transmission, may be involved in certain cognitive dysfunctions in schizophrenia...
  24. ncbi Resazurin assay of radiation response in cultured cells
    S Anoopkumar-Dukie
    Department of Pharmacology and Therapeutics, University College Cork, Ireland
    Br J Radiol 78:945-7. 2005
    ..5 at 60% isoeffect level versus 3.1 for the colony forming assay. Intraday and interday precisions for the resazurin assay were 4.1% and 5.2%, respectively...
  25. pmc Thalidomide increases both intra-tumoural tumour necrosis factor-alpha production and anti-tumour activity in response to 5,6-dimethylxanthenone-4-acetic acid
    Z Cao
    Auckland Cancer Society Research Centre, University of Auckland School of Medicine, New Zealand
    Br J Cancer 80:716-23. 1999
    ..Co-administration of thalidomide may represent a novel approach to improving selective intra-tumoural TNF-alpha production and anti-tumour efficacy of DMXAA...
  26. ncbi Comparison of XTT and Alamar blue assays in the assessment of the viability of various human cancer cell lines by AT-101 (-/- gossypol)
    Selim Uzunoglu
    Section of Molecular Biology, Department of Biology, Faculty of Science and Arts, Celal Bayar University, 45140, Muradiye, Manisa, Turkey
    Toxicol Mech Methods 20:482-6. 2010
    ....
  27. ncbi Detection of inhibitors of phenotypically drug-tolerant Mycobacterium tuberculosis using an in vitro bactericidal screen
    Ian M Bassett
    School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand
    J Microbiol 51:651-8. 2013
    ..Our library screen enabled detection of compounds with a potent level of bactericidal activity towards phenotypically drug-tolerant cultures of M. tuberculosis. ..
  28. ncbi Limitations and relative utility of screening assays to assess engineered nanoparticle toxicity in a human cell line
    N A Monteiro-Riviere
    Center for Chemical Toxicology Research and Pharmacokinetics, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA
    Toxicol Appl Pharmacol 234:222-35. 2009
    ..Therefore, more than one assay may be required when determining nanoparticle toxicity for risk assessment...
  29. ncbi Determination of the dose-depth distribution of proton beam using resazurin assay in vitro and diode laser-induced fluorescence detection
    Min Jung Kim
    Research Institute of Pharmaceutical Sciences and College of Pharmacy, Seoul National University, Seoul 151 742, South Korea
    Anal Chim Acta 593:214-23. 2007
    ....
  30. ncbi A new non-radioactive method for IL-2 bioassay
    K Kwack
    Immunomodulation Research Center, University of Ulsan, Korea
    Mol Cells 10:575-8. 2000
    ..These results demonstrate that the alamarBlue assay is valid for the IL-2 bioassay and that alamarBlue can replace the [3H]thymidine employed in the conventional proliferation assays...
  31. ncbi NF-kappa B activation in vivo in both host and tumour cells by the antivascular agent 5,6-dimethylxanthenone-4-acetic acid (DMXAA)
    S T Woon
    Auckland Cancer Society Research Centre, Faculty of Medical and Health Sciences, Auckland University, Private Bay 92019, New Zealand
    Eur J Cancer 39:1176-83. 2003
    ..Moreover, activation of NF-kappaB in the tumour cell did not confer resistance to DMXAA-induced therapy...
  32. ncbi The use of the microplate alamar blue assay (MABA) to assess the susceptibility of Mycobacterium lepraemurium to anti-leprosy and other drugs
    Melby Mendoza-Aguilar
    Departamento de Inmunologia, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Carpio y Plan de Ayala, Colonia Santo Tomas, 11340, Mexico, D F, Mexico
    J Infect Chemother 18:652-61. 2012
    ..lepraemurium), in response to various drugs, thus offering a method to complement the study of murine leprosy, about which many questions remain unanswered...
  33. pmc Enhancement of the anti-tumour effects of the antivascular agent 5,6-dimethylxanthenone-4-acetic acid (DMXAA) by combination with 5-hydroxytryptamine and bioreductive drugs
    C J Lash
    Department of Pathology, The University of Auckland, New Zealand
    Br J Cancer 78:439-45. 1998
    ..5 days. This study demonstrates that 5-HT and/or bioreductive drugs can improve the therapeutic activity of DMXAA in mice, and that with SN 23816 both approaches can be used together to provide considerably enhanced anti-tumour activity...
  34. ncbi Group II and III mGluRs-mediated presynaptic inhibition of EPSCs recorded from hippocampal interneurons of CA1 stratum lacunosum moleculare
    Christopher J Price
    Medical Research Council, Anatomical Neuropharmacology Unit, Mansfield Road, Oxford OX1 3TH, UK
    Neuropharmacology 49:45-56. 2005
    ..Our results show powerful modulation of excitatory transmission impinging on interneurons of CA1 SLM by presynaptic group II or III mGluRs...
  35. ncbi Design, synthesis and antiviral potential of 14-aryl/heteroaryl-14H-dibenzo[a,j]xanthenes using an efficient polymer-supported catalyst
    Kalla Reddi Mohan Naidu
    Department of Food Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
    Molecules 17:7543-55. 2012
    ..used as an efficient and eco-friendly polymeric catalyst in the synthesis of 14-aryl/heteroaryl-14H-dibenzo[a,j]xanthenes obtained from the reaction of 2-naphthol and carbonyl compounds under solvent-free conditions with short ..
  36. ncbi Retinoic acid induced suicidal erythrocyte death
    Olivier M Niemoeller
    Department of Physiology, University of Tuebingen, Tubingen, Germany
    Cell Physiol Biochem 21:193-202. 2008
    ..The eryptosis could participate in the accelerated clearance of parasitized erythrocytes from circulating blood following treatment with retinoids...
  37. ncbi Interferon-inducible protein 10 induction and inhibition of angiogenesis in vivo by the antitumor agent 5,6-dimethylxanthenone-4-acetic acid (DMXAA)
    Z Cao
    Auckland Cancer Society Research Centre, University of Auckland School of Medicine, New Zealand
    Cancer Res 61:1517-21. 2001
    ..The data support the hypothesis that DMXAA, in addition to antivascular effects mediated by tumor necrosis factor-alpha, may have an antiangiogenic effect mediated largely by the induction of IP-10...
  38. ncbi Signal transduction in red blood cells of the lizards Ameiva ameiva and Tupinambis merianae (Squamata, Teiidae)
    F H Beraldo
    Departamento de Fisiologia, , , , Brazil
    Cell Calcium 29:439-45. 2001
    ..Finally, the purinergic agonist ATP triggers a rapid and transient increase of [Ca2+]c in the cells from both lizard species, mostly by mobilization of the cation from internal stores...
  39. ncbi Vascular attack by 5,6-dimethylxanthenone-4-acetic acid combined with B7.1 (CD80)-mediated immunotherapy overcomes immune resistance and leads to the eradication of large tumors and multiple tumor foci
    J R Kanwar
    Department of Molecular Medicine, School of Medicine and Health Science, University of Auckland, New Zealand
    Cancer Res 61:1948-56. 2001
    ..These findings have important implications for the future direction and utility of cancer immunotherapies aimed at harnessing patients' immune responses to their own tumors...
  40. ncbi Chemical and physiological characterization of fluo-4 Ca(2+)-indicator dyes
    K R Gee
    Molecular Probes, Inc, Eugene, OR 97402, USA
    Cell Calcium 27:97-106. 2000
    ..Because of its higher fluorescence emission intensity, fluo-4 can be used at lower intracellular concentrations, making its use a less invasive practice...
  41. pmc Induction of endothelial cell apoptosis by the antivascular agent 5,6-Dimethylxanthenone-4-acetic acid
    L M Ching
    Auckland Cancer Society Research Centre, University of Auckland School of Medicine, Private Bag 92019, Auckland, New Zealand
    Br J Cancer 86:1937-42. 2002
    ..9 mg x m(-2)). We conclude that 5,6-Dimethylxanthenone-4-acetic acid can induce vascular endothelial cell apoptosis in some murine and human tumours. The action is rapid and appears to be independent of tumour necrosis factor induction...
  42. ncbi Evaluation of hepatic subcellular fractions for Alamar blue and MTT reductase activity
    R J Gonzalez
    Department of Pharmaceutical Sciences, University of the Sciences in Philadelphia, 600 South 43rd Street, PA 19104-4495, Philadelphia, USA
    Toxicol In Vitro 15:257-9. 2001
    ..Therefore, caution should be exercised in ascribing decreases in viability as due solely to mitochondrial damage when using either of these dyes...
  43. pmc Effects of L-type Ca2+ channel antagonism on ventricular arrhythmogenesis in murine hearts containing a modification in the Scn5a gene modelling human long QT syndrome 3
    Glyn Thomas
    Section of Cardiovascular Biology, Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK
    J Physiol 578:85-97. 2007
    ..We conclude that L-type Ca2+ channel antagonism thus exerts specific anti-arrhythmic effects in Scn5a+/Delta hearts through suppression of EADs...
  44. pmc Calcium dynamics of cortical astrocytic networks in vivo
    Hajime Hirase
    Center for Molecular and Behavioral Neuroscience, Rutgers University, Newark, New Jersey, USA
    PLoS Biol 2:E96. 2004
    ..These findings indicate potential neuron-glia communication in the intact brain...
  45. pmc Development of colorimetric microtiter plate assay for assessment of antimicrobials against Acanthamoeba
    James McBride
    Department of Immunology, Strathclyde Institute for Biomedical Sciences, University of Strathclyde, 27 Taylor St, Glasgow, Scotland, UK
    J Clin Microbiol 43:629-34. 2005
    ..As it is based on a standard 96-well, microtiter plate, it is amenable to automation and high throughput...
  46. ncbi Rapid fluorescent assay for screening drugs on Leishmania amastigotes
    Orly Shimony
    The Kuvin Centre for the Study of Infectious and Tropical Diseases, Hebrew University Hadassah Medical School, P O Box 12272, Jerusalem 91220, Israel
    J Microbiol Methods 75:196-200. 2008
    ..This assay only requires a limited number of axenic amastigotes (50,000 cells/well) and can be used to rapidly screen large chemical or natural product libraries for activity against amastigotes...
  47. ncbi Marked potentiation of the antitumour activity of chemotherapeutic drugs by the antivascular agent 5,6-dimethylxanthenone-4-acetic acid (DMXAA)
    Bronwyn G Siim
    Molecular Medicine and Pathology, The University of Auckland, Private Bag 92019, Auckland, New Zealand
    Cancer Chemother Pharmacol 51:43-52. 2003
    ....
  48. ncbi Presynaptic inhibition of corticothalamic feedback by metabotropic glutamate receptors
    Georgia M Alexander
    Neuroscience Program, Wake Forest University School of Medicine, Medical Center Blvd Winston Salem, NC 27157, USA
    J Neurophysiol 94:163-75. 2005
    ..This presynaptic inhibition may partially mute sensory feedback and prevent reentrant excitation from initiating abnormal thalamic rhythms...
  49. pmc Resazurin microtiter assay plate testing of Mycobacterium tuberculosis susceptibilities to second-line drugs: rapid, simple, and inexpensive method
    Anandi Martin
    Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000, Belgium
    Antimicrob Agents Chemother 47:3616-9. 2003
    ..The colorimetric resazurin microtiter assay is inexpensive, rapid, and simple to perform, and implementation of the assay is feasible for low-resource countries...
  50. pmc Gentamicin is primarily localized in vestibular type I hair cells after intratympanic administration
    Sofia Lyford-Pike
    Department of Otolaryngology Head and Neck Surgery, The Johns Hopkins University School of Medicine, 601 North Caroline Street, 6th Floor, Baltimore, MD 21287 0910, USA
    J Assoc Res Otolaryngol 8:497-508. 2007
    ..62 (12.06-13.17) in support cells (p < 0.001, ANOVA). These results suggest that type I hair cells are more susceptible to gentamicin because they more avidly take up or retain the drug in the early period after administration...
  51. pmc Relationship of Ca2+ sparks to STOCs studied with 2D and 3D imaging in feline oesophageal smooth muscle cells
    M T Kirber
    Department of Physiology and Biomedical Imaging Group, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Physiol 531:315-27. 2001
    ..Statistical evaluation of spark clusters disclosed that there were regions of the cell where the probability of spark occurrence was high and others where it was quite low...
  52. ncbi Cell-type specific calcium responses in acute rat hippocampal slices
    Roger Dallwig
    Abteilung fur Allgemeine Zoologie, FB Biologie, Universitat Kaiserslautern, Postfach 3049, D 67653 Kaiserslautern, Germany
    J Neurosci Methods 116:77-87. 2002
    ..81% of the cells with prominent Fluo-4 fluorescence showed responses to low K+, and 86% of these cells were S-100B-positive. Our results suggest that the responsiveness to low K+ can help to identify astrocytes in acute brain slices...
  53. ncbi Imaging of respiratory-related population activity with single-cell resolution
    Frank Funke
    Zentrum Physiologie und Pathophysiologie, Universitat Gottingen, Humboldtallee 23, D 37073 Gottingen, Germany
    Am J Physiol Cell Physiol 292:C508-16. 2007
    ....
  54. pmc Trafficking of systemic fluorescent gentamicin into the cochlea and hair cells
    Qi Wang
    Oregon Hearing Research Center, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    J Assoc Res Otolaryngol 10:205-19. 2009
    ..Trans-strial trafficking of aminoglycosides from strial capillaries to marginal cells will be dependent on as-yet-unidentified mechanisms that convey these drugs across the intra-strial electrical barrier and into marginal cells...
  55. pmc Cytoplasmic and intra-nuclear binding of gentamicin does not require endocytosis
    Sigrid E Myrdal
    Oregon Hearing Research Center, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    Hear Res 204:156-69. 2005
    ....
  56. ncbi In vitro assays for evaluating the ultraviolet B-induced damage in cultured human retinal pigment epithelial cells
    Hyun Yi Youn
    School of Optometry, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, Canada N2L 3G1
    J Photochem Photobiol B 88:21-8. 2007
    ....
  57. ncbi Inhibition of human CYP3A4, UGT1A6, and P-glycoprotein with halogenated xanthene food dyes and prevention by superoxide dismutase
    Kenji Furumiya
    Department of Drug Metabolism and Disposition, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan
    J Toxicol Environ Health A 71:1307-13. 2008
    ..It is possible that red cosmetics containing phloxine, erythrosine, or rose bengal react with proteins in skin and may lead to skin damage...
  58. ncbi Enhancement of tumor radiation response by the antivascular agent 5,6-dimethylxanthenone-4-acetic acid
    W R Wilson
    Department of Pathology, The University of Auckland, New Zealand
    Int J Radiat Oncol Biol Phys 42:905-8. 1998
    ..This investigation examines activity of DMXAA as a radiation modifier using two murine tumors...
  59. ncbi Plasmodium falciparum malaria parasites display a THG-sensitive Ca2+ pool
    Fernando P Varotti
    Departamento de Fisiologia, Instituto de Biociencias, Universidade de Sao Paulo, Sao Paulo, Brazil
    Cell Calcium 33:137-44. 2003
  60. ncbi The phototoxicity of xanthene derivatives against Escherichia coli, Staphylococcus aureus, and Saccharomyces cerevisiae
    Hong Wang
    School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
    Curr Microbiol 52:1-5. 2006
    ..Our results showed xanthenes had the potential to act as alternatives to conventional antimicrobial compounds and also could be used for the ..
  61. ncbi Effects of 5,6-dimethylxanthenone-4-acetic acid on human tumor microcirculation assessed by dynamic contrast-enhanced magnetic resonance imaging
    Susan M Galbraith
    Department of Medical Oncology and Paul Strickland Scanner Centre, Mount Vernon Hospital, Northwood, Middlesex, United Kingdom
    J Clin Oncol 20:3826-40. 2002
    ..Dynamic contrast-enhanced (DCE) magnetic resonance imaging (MRI) studies were performed in the phase I trials to examine changes related to blood flow and permeability in tumor and muscle...
  62. pmc TRPV1 regulators mediate gentamicin penetration of cultured kidney cells
    Sigrid E Myrdal
    Oregon Hearing Research Center, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    Hear Res 204:170-82. 2005
    ..In addition, GTTR uptake was blocked by RTX in the presence of more physiological levels (2 mM) of Ca2+. Thus gentamicin enters cells via cation channels, and gentamicin uptake can be modulated by regulators of the TRPV1 channel...
  63. ncbi Microplate Alamar blue assay for susceptibility testing of Candida albicans biofilms
    Kimberly K Repp
    Cancer Research Institute, Arizona State University, Tempe, Arizona, USA
    Med Mycol 45:603-7. 2007
    ..88-0.93) and CFU/ml (r=0.93-0.99) for all four C. albicans test strains. This simple, reproducible method for determining in vitro drug susceptibility should facilitate discovery of antifungals active against Candida biofilms...
  64. pmc Asynchronous transmitter release: control of exocytosis and endocytosis at the salamander rod synapse
    F Rieke
    Department of Pharmacological and Physiological Sciences, University of Chicago, IL 60637, USA
    J Physiol 493:1-8. 1996
    ..7 s by compensatory changes in the rate of endocytosis. 5. Vesicle cycling in the rod synapse is specialized for graded transmission and differs from that previously described for synapses that release synchronized bursts of transmitter...
  65. ncbi Cytotoxicity of endogenous isoquinolines to human dopaminergic neuroblastoma SH-SY5Y cells
    T Takahashi
    Department of Food and Nutrition, Konan Women s College, Japan
    J Neural Transm 104:59-66. 1997
    ..The involvement of 1,2-DMDHIQ+ to the neurotoxicity of NM(R)Sal was suggested and discussed...
  66. pmc Uptake of fluorescent gentamicin by vertebrate sensory cells in vivo
    C F Dai
    Oregon Hearing Research Center, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    Hear Res 213:64-78. 2006
    ..GTTR uptake by hair cells in vivo suggests that GTTR has potential to elucidate aminoglycoside transport mechanisms into the inner ear, and as a bio-tracer for in vivo pharmacokinetic studies...
  67. ncbi Assessing the potential of fish cell lines as tools for the cytotoxicity testing of estuarine sediment aqueous elutriates
    M Davoren
    Radiation and Environmental Science Centre, Focas Institute, Dublin Institute of Technology, Kevin Street, Dublin 8, Ireland
    Toxicol In Vitro 19:421-31. 2005
    ..In order to fully evaluate the overall sensitivity of this cell line, further research is warranted using an extensive range of test sites incorporating more polluted sediments...
  68. ncbi Eco-friendly and efficient one-pot synthesis of alkyl- or aryl-14H-dibenzo[a,j]xanthenes in water
    Minoo Dabiri
    Department of Chemistry, Faculty of Science, Shahid Beheshti University, Postal Code 1983963113, Evin, Tehran, Iran
    Bioorg Med Chem Lett 18:436-8. 2008
    ..Several solvents were examined for this reaction; however, in terms of reaction yield and time, water was found to be the optimum solvent...
  69. pmc A systemic gentamicin pathway across the stria vascularis
    Chun Fu Dai
    Oregon Hearing Research Center, Oregon Health Sciences University, Portland, OR 97239, USA
    Hear Res 235:114-24. 2008
    ....
  70. ncbi The antitumour agent 5,6-dimethylxanthenone-4-acetic acid acts in vitro on human mononuclear cells as a co-stimulator with other inducers of tumour necrosis factor
    M Philpott
    Auckland Cancer Society Research Centre, University of Auckland School of Medicine, Auckland, New Zealand
    Eur J Cancer 37:1930-7. 2001
    ..Taken together, the results indicate DMXAA acts in vitro on HPBL to co-stimulate TNF production by a wide variety of agents, and suggests that IKK is the target that mediates this action...
  71. ncbi Increased plasma serotonin following treatment with flavone-8-acetic acid, 5,6-dimethylxanthenone-4-acetic acid, vinblastine, and colchicine: relation to vascular effects
    B C Baguley
    Cancer Research Laboratory, University of Auckland School of Medicine, New Zealand
    Oncol Res 9:55-60. 1997
    ....
  72. ncbi Development and application of a resazurin-based biomass activity test for activated sludge plant management
    Bridget P McNicholl
    Northern Ireland Water Service, Northland House, Belfast, Northern Ireland
    Water Res 41:127-33. 2007
    ..The method has been used to assess the impact of trade wastes on the activities of two municipal activated sludge populations of differing characteristics...
  73. ncbi Effects of the serotonin receptor antagonist cyproheptadine on the activity and pharmacokinetics of 5,6-dimethylxanthenone-4-acetic acid (DMXAA)
    L Zhao
    Auckland Cancer Society Research Centre, Faculty of Medical and Health Sciences, The University of Auckland, New Zealand
    Cancer Chemother Pharmacol 47:491-7. 2001
    ..However, serum TNF concentrations were not increased, suggesting that the increased anti-tumour effects are mediated by an increased local tumour response, arising from the extended tumour DMXAA concentrations...
  74. ncbi Combining bioreductive drugs (SR 4233 or SN 23862) with the vasoactive agents flavone acetic acid or 5,6-dimethylxanthenone acetic acid
    S Cliffe
    Department of Pathology, University of Auckland School of Medicine, New Zealand
    Int J Radiat Oncol Biol Phys 29:373-7. 1994
    ....
  75. ncbi Oral activity and pharmacokinetics of 5,6-dimethylxanthenone-4-acetic acid (DMXAA) in mice
    Liangli Zhao
    Auckland Cancer Society Research Centre, Faculty of Medical and Health Sciences, The University of Auckland, New Zealand
    Cancer Chemother Pharmacol 49:20-6. 2002
    ..Since oral administration has many advantages, we compared the biological activity and pharmacokinetics of DMXAA in mice following oral and intraperitoneal (i.p.) administration...
  76. pmc Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis
    Juan Carlos Palomino
    Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat 155, Antwerp 2000, Belgium
    Antimicrob Agents Chemother 46:2720-2. 2002
    ..Specificity and sensitivity were excellent. The method is simple, inexpensive, and rapid and might be used with other antituberculosis drugs...
  77. ncbi Measurement of plasma 5-hydroxyindoleacetic acid as a possible clinical surrogate marker for the action of antivascular agents
    P Kestell
    Auckland Cancer Society Research Centre, University of Auckland Medical School, Auckland Hospital, Private Bag 92019, Auckland, New Zealand
    Clin Chim Acta 314:159-66. 2001
    ....
  78. pmc Effect of thalidomide on tumour necrosis factor production and anti-tumour activity induced by 5,6-dimethylxanthenone-4-acetic acid
    L M Ching
    Cancer Research Laboratory, University of Auckland School of Medicine, New Zealand
    Br J Cancer 72:339-43. 1995
    ..The results suggest a possible new application for thalidomide and pose new questions about the action of 5,6-MeXAA and related compounds...
  79. pmc Production of tumour necrosis factor-alpha by cultured human peripheral blood leucocytes in response to the anti-tumour agent 5,6-dimethylxanthenone-4-acetic acid (NSC 640488)
    M Philpott
    Cancer Research Laboratory, University of Auckland School of Medicine, New Zealand
    Br J Cancer 76:1586-91. 1997
    ..In vitro analysis of the response of human peripheral blood leucocytes to DMXAA may be a useful test in clinical trials of agents such as DMXAA...
  80. ncbi Melatonin protects against ischaemic-reperfusion myocardial damage
    R Salie
    MRC Experimental Biology Programme, University of Stellenbosch, Republic of South Africa
    J Mol Cell Cardiol 33:343-57. 2001
    ..CONCLUSION: Melatonin effectively reduced damage induced by chemical hypoxia in adult cardiomyocytes, probably by virtue of its effects on reactive oxygen species generation and intracellular Ca2+ accumulation...
  81. pmc Inositol-1,4,5-trisphosphate-mediated spontaneous activity in mouse embryonic stem cell-derived cardiomyocytes
    Nidhi Kapur
    Department of Physiology, Stritch School of Medicine, Loyola University Chicago, 2160 South First Avenue, Maywood, IL 60153, USA
    J Physiol 581:1113-27. 2007
    ..The results of this study offer important insight into the role of IP3R-mediated Ca2+ release for pacemaker activity in differentiating cardiomyocytes...
  82. ncbi Spatially confined diffusion of calcium in dendrites of hippocampal neurons revealed by flash photolysis of caged calcium
    Eduard Korkotian
    Department of Neurobiology, The Weizmann Institute, Rehovot, Israel
    Cell Calcium 40:441-9. 2006
    ....
  83. ncbi Optical mapping of V(m) and Ca(i)(2+) in a model of arrhythmias induced by local catecholamine application in patterned cell cultures
    David Z Lan
    Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL, USA
    Pflugers Arch 453:871-7. 2007
    ..The location of the arrhythmia source correlated with localized Ca(i)(2+) oscillations suggesting that arrhythmias were caused by Ca(i)(2+) overload at these locations...
  84. ncbi Sphingosine 1-phosphate induces Ca2+ transients and cytoskeletal rearrangement in C2C12 myoblastic cells
    Lucia Formigli
    Department of Anatomy, Histology, and Forensic Medicine, University of Florence, 50134 Florence, Italy
    Am J Physiol Cell Physiol 282:C1361-73. 2002
    ..This event, however, was unrelated to [Ca2+]i increase, because the two phenomena were temporally shifted. We propose that SPP may promote C2C12 cell contraction through Ca2+-independent mechanisms...
  85. ncbi The effect of unoprostone isopropyl on Ca2+ release-activated Ca2+ currents in cultured monkey trabecular meshwork cells and ciliary muscle cells
    Masahiko Shimura
    Department of Ophthalmology, NTT East Japan Tohoku Hospital, and Department of Ophthalmology and Medical Sciences, Tohoku University Graduate School of Medicine, Miyagi, Japan
    J Ocul Pharmacol Ther 22:219-26. 2006
    ....
  86. ncbi An increase in calcium influx contributes to post-tetanic potentiation at the rat calyx of Held synapse
    Ron L P Habets
    Erasmus MC, University Medical Center Rotterdam, Dr Molewaterplein 50, 3015 GE Rotterdam, The Netherlands
    J Neurophysiol 96:2868-76. 2006
    ..Our data therefore suggest that the increase of release probability during PTP at the calyx of Held synapse is largely explained by an increase in the calcium influx per action potential...
  87. ncbi 'In situ' high pressure confocal Ca(2+)-fluorescence microscopy in skeletal muscle: a new method to study pressure limits in mammalian cells
    O Friedrich
    Medical Biophysics, Institute of Physiology and Pathophysiology, INF 326, Ruprecht Karls University, Heidelberg, Germany
    Undersea Hyperb Med 33:181-95. 2006
    ..The pressure induced disturbance of Ca2+ homeostasis might have important implications for the pressure exposure limits and/or dive profiles of deep sea mammals...
  88. ncbi Lung surfactant secretion by interalveolar Ca2+ signaling
    Hideo Ichimura
    Lung Biology Laboratory, Department of Physiology and Cellular Biophysics, College of Physicians and Surgeons, Columbia University, St Luke s Roosevelt Hospital Center, New York, NY 10019, USA
    Am J Physiol Lung Cell Mol Physiol 291:L596-601. 2006
    ..We conclude that Cx43-dependent, interalveolar Ca2+ signals regulate type 2 cell secretion in adjacent alveoli. Such interalveolar communication might facilitate acinar coordination of alveolar function...
  89. ncbi Intracellular calcium regulation in inner hair cells from neonatal mice
    H J Kennedy
    Department of Physiology, School of Medical Sciences, University of Bristol, UK
    Cell Calcium 31:127-36. 2002
    ..Together, these data indicate that both thapsigargin-sensitive stores and mitochondria can take up Ca(2+)(i), but that Ca(2+) efflux from the cell occurs solely via a plasma membrane Ca(2+)-ATPase...
  90. ncbi Cadmium is acutely toxic for murine hepatocytes: effects on intracellular free Ca(2+) homeostasis
    S S Wang
    College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu, China
    Physiol Res 56:193-201. 2007
    ..Ca(2+)](i) elevation in acutely cadmium-exposed hepatocytes is closely related to the extracellular Ca(2+) entry and an excessive release of Ca(2+) from intracellular stores...
  91. ncbi Protective effect of arachidonic acid on glutamate neurotoxicity in rat retinal ganglion cells
    Atsushi Kawasaki
    Departments of Ophthalmology and Visual Science, Yale University School of Medicine, New Haven, Connecticut 06520 8061, USA
    Invest Ophthalmol Vis Sci 43:1835-42. 2002
    ..The purpose of this study was to test the hypothesis that AA could protect RGCs from glutamate neurotoxicity...
  92. ncbi Intracellular Ca2+ signals evoked by stimulation of nicotinic acetylcholine receptors in SH-SY5Y cells: contribution of voltage-operated Ca2+ channels and Ca2+ stores
    Federico A Dajas-Bailador
    Department of Biology and Biochemistry, University of Bath, Bath, UK
    J Neurochem 81:606-14. 2002
    ..Moreover, the alpha7, but not alpha3beta2* nAChR, are responsible for a fraction of the VOCC-independent nicotine-evoked Ca2+ increase that appears to be functionally coupled to ryanodine sensitive Ca2+ stores...
  93. ncbi Pharmacology and functional properties of NTS2 neurotensin receptors in cerebellar granule cells
    Philippe Sarret
    Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec H3A 2B4, Canada
    J Biol Chem 277:36233-43. 2002
    ..Taken together, the present results demonstrate that NTS2 receptors are present both presynaptically and postsynaptically in central neurons and that NT and levocabastine act as agonists on these receptors...
  94. ncbi Neuropeptide Y (NPY) and NPY receptors in the cardiovascular system: implication in the regulation of intracellular calcium
    Danielle Jacques
    Department of Anatomy and Cell Biology, University of Sherbrooke, Sherbrooke, Canada
    Can J Physiol Pharmacol 85:43-53. 2007
    ..This suggests the ability of NPY to contribute to the regulation of the excitation-secretion coupling of EECs and the excitation-contraction coupling of cardiomyocytes and vascular smooth muscle cells...
  95. pmc Vesicular ATP is the predominant cause of intercellular calcium waves in astrocytes
    David N Bowser
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, UK
    J Gen Physiol 129:485-91. 2007
    ..In contrast, metabotropic glutamate receptors and gap junctions do not contribute significantly to calcium wave propagation. Our data suggest that endogenous extracellular astrocytic ATP can signal over broad spatiotemporal scales...
  96. ncbi Astrocytes in 17beta-estradiol treated mixed hippocampal cultures show attenuated calcium response to neuronal activity
    Shilpa P Rao
    Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India
    Glia 53:817-26. 2006
    ..Since astrocytes can modulate synaptic transmission by release of glutamate, the attenuated ([Ca(2+)](i)) response seen following estradiol treatment could have functional consequences on astrocyte-neuron signaling...
  97. ncbi Sparks and puffs in oligodendrocyte progenitors: cross talk between ryanodine receptors and inositol trisphosphate receptors
    L L Haak
    National Institute of Child Health and Human Development, National Institutes of Health (NIH, Bethesda, Maryland 20892, USA
    J Neurosci 21:3860-70. 2001
    ..Thus, in OPs, interactions between IP(3)Rs and RyRs determine the spatial and temporal characteristics of calcium signaling, from microdomains to intracellular waves...
  98. pmc Local Ca(2+) transients and distribution of BK channels and ryanodine receptors in smooth muscle cells of guinea-pig vas deferens and urinary bladder
    Y Ohi
    Department of Molecular and Cellular Pharmacology, Faculty of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabedori, Mizuhoku, Nagoya 467-8603, Japan
    J Physiol 534:313-26. 2001
    ..BK channels and RyRs may co-localize densely at the junctional areas of plasmalemma and SR fragments, where Ca(2+) sparks occur to elicit STOCs...
  99. ncbi Altered cardiac sarcoplasmic reticulum function of intact myocytes of rat ventricle during metabolic inhibition
    C L Overend
    Department of Medicine, University of Manchester, Manchester, UK
    Circ Res 88:181-7. 2001
    ..In the context of ischemia, this extra accumulation of Ca(2+) would present a risk of potentially arrhythmogenic, spontaneous release of Ca(2+) on reperfusion of the tissue...
  100. ncbi Dual purinergic synaptic transmission in the human enteric nervous system
    J E Wunderlich
    Department of Anesthesiology, College of Medicine and Public Health, The Ohio State University, Columbus, OH 43210, USA
    Am J Physiol Gastrointest Liver Physiol 294:G554-66. 2008
    ..Inhibitory AdoA(3)R inhibit nucleotide or cholinergic transmission in the huENS...
  101. ncbi Remodeling of synaptic actin induced by photoconductive stimulation
    M A Colicos
    Neurobiology Section/Division of Biology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Cell 107:605-16. 2001
    ..Our results indicate that activity-dependent presynaptic structural plasticity facilitates the formation of new active presynaptic terminals...

Research Grants47

  1. Synthesis of Macrolides, Steroids, Cyclopentanoids, etc.
    BARRY TROST; Fiscal Year: 2009
    ..These new synthetic methods apply to many structural types beyond those illustrated and constitutes a significant to gain access to complex molecular targets more easily. ..
  2. RADIATION AND DRUG EFFECTS ON TUMOR CELL SUBPOPULATIONS
    Dietmar Siemann; Fiscal Year: 2001
    ..Ultimately we hope that these approaches can be reliably developed to an extent where the use of human tumor biopsy material may lead to radiotherapy treatments more tailored to the individual patient. ..
  3. PERIPHERAL SOMATOSTATIN CONTROLS INFLAMMATORY PAIN
    Susan Carlton; Fiscal Year: 2004
    ..Peripheral SST receptors offer novel targets for nociceptor modulation and are likely targets for further development of non-opioid therapies to aid in reducing the pain and long-term deleterious changes that can accompany inflammation. ..
  4. Non-Peptide Somatostatin Agonist Analgesics
    Susan Carlton; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  5. Ca2+ Waves and Arrhythmias
    PENELOPE BOYDEN; Fiscal Year: 2008
    ..abstract_text> ..
  6. Genetics of Longevity and Age-Sensitive Traits in Mice
    Richard Miller; Fiscal Year: 2008
    ..abstract_text> ..
  7. Perinatal infection, cytokines and brain injury
    Zhengwei Cai; Fiscal Year: 2008
    ....
  8. Aminoglycoside uptake and clearance in mammalian cells
    Takatoshi Karasawa; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  9. Perinatal hypoxia-ischemia, glutamate receptors, and Nitric Oxide Synthase
    Zhengwei Cai; Fiscal Year: 2007
    ..Direct effects of inflammatory cytokines and IGF-1 on survival and differentiation of OLs will be examined in cultures of OLs at various maturation stages. ..
  10. IAPs As Novel Targets for Cancer Therapy
    Lily Yang; Fiscal Year: 2007
    ..Results from this research should allow us to determine if IAPs are novel molecular targets for the development of cancer-specific therapeutic approaches. ..
  11. MODELS FOR TESTING CANDIDATE TOPICAL MICROBICIDES FOR CYTOTOXICITY AND ACTIVITY A
    Mary Lampe; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  12. Control of Cell Proliferation by Runx Proteins
    JAMES COFFMAN; Fiscal Year: 2009
    ..purpuratus genome sequence, which will greatly facilitate both the cis-regulatory analysis of genes and the identification of purified proteins by mass spectrometry. ..
  13. Regulation of Secretory Ion Channels in Colonic Crypts
    DAN HALM; Fiscal Year: 2009
    ..Thus, this project will lead to greater insight into the regulatory control of colonic fluid secretion and likely to therapeutic interventions for patients with life-threatening secretory activity. ..
  14. Regulation of Bile Ductular Proliferation
    JONATHAN DRANOFF; Fiscal Year: 2009
    ..We anticipate that the results of these proposed experiments will lead to novel pharmacologic approaches for the regulation of bile ductular proliferation in biliary cirrhosis. ..
  15. Redox-sensitive developmental pathways and gene regulatory networks
    JAMES COFFMAN; Fiscal Year: 2009
    ..Finally, the Comparative Toxicogenomics Database (CTD) at MDIBL will be used to determine the relevance of the pathways discovered in sea urchins to human health, and to generate hypotheses that might explain specific human diseases. ..
  16. Calcium Signaling, Metabolism, and EC Coupling in Heart
    JOSHUA GOLDHABER; Fiscal Year: 2008
    ..abstract_text> ..
  17. Ion Channel Function in Arrhythmogenic Cardiac Cell
    PENELOPE ALTMAN BOYDEN; Fiscal Year: 2010
    ....
  18. Low-Level Prenatal Lead Exposure and Retinal Toxicity
    Donald Fox; Fiscal Year: 2009
    ....
  19. Nucleus Accumbens-Mediated Feeding: Output Pathways
    Thomas R Stratford; Fiscal Year: 2010
    ....
  20. SYNAPTIC VESICLE TRAFFICKING IN HIPPOCAMPAL SYNAPSES
    Venkatesh N Murthy; Fiscal Year: 2010
    ..These experiments will elucidate fundamental presynaptic mechanisms of vesicle traffic in GABAergic synapses and how they are altered by activity. ..
  21. METABOLISM OF SERUM AMYLOID A (SAA:HDL)
    Barbara Kluve Beckerman; Fiscal Year: 2007
    ..By clarifying pathogenic mechanisms at the cellular level, these studies will help identify potential targets for drugs aimed at inhibiting amyloid formation or arresting its progression. ..
  22. Prefrontal Glutamate and Working Memory
    Mary Gregory; Fiscal Year: 2006
    ..This study proposes to investigate the effects of differential housing on glutamatergic transmission within the PFC and the relationship of these changes to working memory. ..
  23. PARACRINE REGULATION OF BILE DUCT SECRETION BY ATP
    JONATHAN DRANOFF; Fiscal Year: 2004
    ..The Yale School of Medicine Department of Internal Medicine has pledged that the candidate will have extensive protected research time and excellent resources for the duration of the award. ..
  24. WILD DERIVED MOUSE STOCKS--NEW MODELS FOR AGING RESEARCH
    Richard Miller; Fiscal Year: 2004
    ....
  25. DEVELOPMENT OF CRYOPLATES FOR CYTOTOXICITY TESTING
    Lia Campbell; Fiscal Year: 2004
    ..It is anticipated that optimization of procedures for corneal endothelial cells will provide a technology platform that can be applied, with further development, to other cell types. ..
  26. EXCITATION-CONTRACTION COUPLING IN STRIATED MUSCLE
    STEPHEN BAYLOR; Fiscal Year: 2005
    ..Overall, the knowledge obtained will contribute importantly to understanding the mechanisms of intracellular Ca signaling and the mechanisms by which specific drugs and diseases affect muscle performance. ..
  27. NTPDase2 expression and function in the liver
    JONATHAN DRANOFF; Fiscal Year: 2005
    ..The experiments proposed in these specific aims will provide the basis of several new lines of liver disease research and should provide novel therapeutic targets for treatment of cholestatic and fibrotic liver disease. ..
  28. GENETICS OF AGE SENSITIVE TRAITS IN MICE
    Richard Miller; Fiscal Year: 2003
    ..At best, the program may permit the localization of loci that influence multiple age-sensitive domains and are thus plausible candidates for genes that regulate the rate of aging per se. ..
  29. CORTICAL OSTEOPONTIN EXPRESSION IN HYDRONEPHROSIS
    RUSSELL SCADUTO; Fiscal Year: 2002
    ..In these studies the renin angiotensin system will be manipulated with the use of an ACE inhibitor, enalapril, and an AT-1 blocker, losartan. ..
  30. MOLECULAR ANGLE TRANSITIONS IN MUSCLE
    THOMAS BURGHARDT; Fiscal Year: 2001
    ....
  31. Regulation and actions of NO in the cardiac plexus
    JEAN HARDWICK; Fiscal Year: 2005
    ..In particular, it will provide important information concerning the regulation and of NO in the control of cardiac function. ..
  32. Microvascular Permeability and Sex
    VIRGINIA HUXLEY; Fiscal Year: 2008
    ....
  33. PRESERVATION OF LIVER SPHEROIDS
    Lia Campbell; Fiscal Year: 2006
    ..Phase II studies will involve optimization for the best method of cryopreservation by either freezing or vitrification in anticipation of commercialization of mammalian liver spheroids. [unreadable] [unreadable] [unreadable]..
  34. Development of PET Radioligands for NMDA Receptors
    Rikki Waterhouse; Fiscal Year: 2004
    ..The data obtained will be pivotal for the design of studies to non-invasively assess NMDA receptor function and will identify suitable radiotracers and provide preliminary data to support future grant submissions. ..
  35. Auditory thalamocortical integration, in vitro
    Heather Rose; Fiscal Year: 2003
    ..By increasing the understanding of cortical mechanisms in processing, auditory stimuli researchers will be able to advance treatments for the hearing impaired. ..
  36. F.nucleatum in Amniotic Fluid Infection
    Yiping Han; Fiscal Year: 2004
    ..nucleatum for diagnostic purposes, and intervention studies aimed at reducing the incidence of preterm birth. ..
  37. METABOTROPIC GLUTAMATE RECEPTORS IN BASAL GANGLIA
    COLLEEN NISWENDER; Fiscal Year: 2009
    ..Furthermore, we will test the hypothesis that agonists or allosteric potentiators of this receptor can provide palliative relief in rodent models of PD by actions in the GP. ..
  38. Sonoporation as a new genetic tool for F. nucleatum
    Yiping Han; Fiscal Year: 2008
    ..nucleatum will be investigated. Our long term goal is to develop sonoporation into a robust tool for genetic manipulation of microorganisms [unreadable] [unreadable] [unreadable]..
  39. Measurement of GPCR-mediated thallium flux:GIRK (RMI)
    COLLEEN NISWENDER; Fiscal Year: 2005
    ..Identified compounds will provide new pharmacological tools to test the role of the ?2C adrenergic receptor in psychiatric and cardiovascular disorders and the role of GIRK 1/2 channels in the modulation of neuronal excitability. ..
  40. Assay for HTS of Gi/Go-linked GPCRs: mGluR7 as Prot(RMI)
    COLLEEN NISWENDER; Fiscal Year: 2007
    ..abstract_text> ..
  41. A new tumor marker for Ovarian Cancer
    Madhwa Raj; Fiscal Year: 2007
    ..The RCP test will be a valuable application of the use of cancer cell signatures as detection markers, which is a critical component of the 2001 Bypass Budget Document of NCI. ..
  42. Comparative In Vivo Biodistribution of Characterized Manufactured Nanomaterials
    Nancy A Monteiro Riviere; Fiscal Year: 2010
    ..These coordinated studies will provide data to link physical chemical characterizations to in vivo MNM disposition. ..
  43. NEURAL CIRCUITRY MEDIATING SUCROSE INDUCED ANALGESIA
    Matthew Ennis; Fiscal Year: 2003
    ..This work will lead to a better understanding of neural circuits controlling taste and pain and how these circuits regulate analgesia in newborns. ..
  44. MECHANISMS OF WOUND HEALING WITH CULTURED HUMAN SKIN
    Steven Boyce; Fiscal Year: 2005
    ..Accomplishment of these objectives will contribute to reduced mortality and morbidity from burns, improved materials for plastic and reconstructive surgery, and development of other tissue and organ substitutes. ..
  45. Somatic Stem Cells in Engineered Human Skin
    Steven Boyce; Fiscal Year: 2009
    ....