sulfotransferases

Summary

Summary: Enzymes which transfer sulfate groups to various acceptor molecules. They are involved in posttranslational sulfation of proteins and sulfate conjugation of exogenous chemicals and bile acids. EC 2.8.2.

Top Publications

  1. ncbi Sulfotransferases: structure, mechanism, biological activity, inhibition, and synthetic utility
    Eli Chapman
    Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Angew Chem Int Ed Engl 43:3526-48. 2004
  2. ncbi Human sulfotransferases and their role in chemical metabolism
    Niranjali Gamage
    School of Biomedical Sciences, School of Molecular and Microbial Sciences, and Institute for Molecular Bioscience, University of Queensland, Queensland 4072, Australia
    Toxicol Sci 90:5-22. 2006
  3. ncbi Maintenance of chondroitin sulfation balance by chondroitin-4-sulfotransferase 1 is required for chondrocyte development and growth factor signaling during cartilage morphogenesis
    Michael Klüppel
    Programme in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
    Development 132:3989-4003. 2005
  4. ncbi Tyrosine sulfation of the amino terminus of CCR5 facilitates HIV-1 entry
    M Farzan
    Dana Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 96:667-76. 1999
  5. pmc Iduronic acid in chondroitin/dermatan sulfate: biosynthesis and biological function
    Anders Malmstrom
    Department of Experimental Medical Science, Biomedical Center D12, Lund University, Lund, Sweden
    J Histochem Cytochem 60:916-25. 2012
  6. ncbi Multiple isozymes of heparan sulfate/heparin GlcNAc N-deacetylase/GlcN N-sulfotransferase. Structure and activity of the fourth member, NDST4
    J Aikawa
    Department of Cellular and Molecular Medicine, Glycobiology Research and Training Center, University of California, San Diego, La Jolla, California 92093 0687, USA
    J Biol Chem 276:5876-82. 2001
  7. ncbi The biology and enzymology of protein tyrosine O-sulfation
    Kevin L Moore
    Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, The Department of Medicine, University of Oklahoma Health Sciences Center, USA
    J Biol Chem 278:24243-6. 2003
  8. ncbi Secreted peptide signals required for maintenance of root stem cell niche in Arabidopsis
    Yo Matsuzaki
    Graduate School of Bio agricultural Sciences, Nagoya University, Chikusa, Nagoya 464 8601, Japan
    Science 329:1065-7. 2010
  9. ncbi Human N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase cDNA is related to human B cell recombination activating gene-associated gene
    S Ohtake
    Department of Life Science, Aichi University of Education, Kariya, Aichi 448 8542, Japan
    J Biol Chem 276:43894-900. 2001
  10. pmc Mice deficient in N-acetylgalactosamine 4-sulfate 6-o-sulfotransferase are unable to synthesize chondroitin/dermatan sulfate containing N-acetylgalactosamine 4,6-bissulfate residues and exhibit decreased protease activity in bone marrow-derived mast cells
    Shiori Ohtake-Niimi
    Department of Chemistry, Aichi University of Education, Igaya cho, Kariya, Aichi 448 8542, Japan
    J Biol Chem 285:20793-805. 2010

Detail Information

Publications314 found, 100 shown here

  1. ncbi Sulfotransferases: structure, mechanism, biological activity, inhibition, and synthetic utility
    Eli Chapman
    Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Angew Chem Int Ed Engl 43:3526-48. 2004
    ..Early studies of sulfotransferases (STs), the enzymes that catalyze sulfonation, focused primarily on the cytosolic STs, which are involved in ..
  2. ncbi Human sulfotransferases and their role in chemical metabolism
    Niranjali Gamage
    School of Biomedical Sciences, School of Molecular and Microbial Sciences, and Institute for Molecular Bioscience, University of Queensland, Queensland 4072, Australia
    Toxicol Sci 90:5-22. 2006
    ..A supergene family of enzymes called sulfotransferases (SULTs) catalyze this reaction...
  3. ncbi Maintenance of chondroitin sulfation balance by chondroitin-4-sulfotransferase 1 is required for chondrocyte development and growth factor signaling during cartilage morphogenesis
    Michael Klüppel
    Programme in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
    Development 132:3989-4003. 2005
    ..process that includes the attachment of sulfate groups to specific positions of the polysaccharide chains by sulfotransferases. Heparan-sulfate and heparan sulfate-sulfotransferases play important roles in growth factor signaling and ..
  4. ncbi Tyrosine sulfation of the amino terminus of CCR5 facilitates HIV-1 entry
    M Farzan
    Dana Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 96:667-76. 1999
    ..CXCR4, another important HIV-1 coreceptor, is also sulfated. Tyrosine sulfation may contribute to the natural function of many 7TMS receptors and may be a modification common to primate immunodeficiency virus coreceptors...
  5. pmc Iduronic acid in chondroitin/dermatan sulfate: biosynthesis and biological function
    Anders Malmstrom
    Department of Experimental Medical Science, Biomedical Center D12, Lund University, Lund, Sweden
    J Histochem Cytochem 60:916-25. 2012
    ..This short review presents the biosynthesis and functions of iduronic acid-containing structures, also as revealed by the analysis of the DS-epi1- and 2-deficient mouse models...
  6. ncbi Multiple isozymes of heparan sulfate/heparin GlcNAc N-deacetylase/GlcN N-sulfotransferase. Structure and activity of the fourth member, NDST4
    J Aikawa
    Department of Cellular and Molecular Medicine, Glycobiology Research and Training Center, University of California, San Diego, La Jolla, California 92093 0687, USA
    J Biol Chem 276:5876-82. 2001
    ....
  7. ncbi The biology and enzymology of protein tyrosine O-sulfation
    Kevin L Moore
    Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, The Department of Medicine, University of Oklahoma Health Sciences Center, USA
    J Biol Chem 278:24243-6. 2003
  8. ncbi Secreted peptide signals required for maintenance of root stem cell niche in Arabidopsis
    Yo Matsuzaki
    Graduate School of Bio agricultural Sciences, Nagoya University, Chikusa, Nagoya 464 8601, Japan
    Science 329:1065-7. 2010
    ..The RGFs function independently of the auxin pathway. These peptide signals play a crucial role in postembryonic root development...
  9. ncbi Human N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase cDNA is related to human B cell recombination activating gene-associated gene
    S Ohtake
    Department of Life Science, Aichi University of Education, Kariya, Aichi 448 8542, Japan
    J Biol Chem 276:43894-900. 2001
    ..The nucleotide sequence of the human GalNAc4S-6ST cDNA was nearly identical to the sequence of human B cell recombination activating gene-associated gene...
  10. pmc Mice deficient in N-acetylgalactosamine 4-sulfate 6-o-sulfotransferase are unable to synthesize chondroitin/dermatan sulfate containing N-acetylgalactosamine 4,6-bissulfate residues and exhibit decreased protease activity in bone marrow-derived mast cells
    Shiori Ohtake-Niimi
    Department of Chemistry, Aichi University of Education, Igaya cho, Kariya, Aichi 448 8542, Japan
    J Biol Chem 285:20793-805. 2010
    ..These observations suggest that CS containing GalNAc(4,6-SO(4)) residues in BMMCs may contribute to retain the active proteases in the granules of BMMCs but not for the maturation of BMMCs into connective tissue-type mast cells...
  11. ncbi Reassessment of the toxin profile of Cylindrospermopsis raciborskii T3 and function of putative sulfotransferases in synthesis of sulfated and sulfonated PSP toxins
    Katia Soto-Liebe
    Pontificia Universidad Catolica de Chile, Alameda 340, 6513492 Santiago, Chile
    Toxicon 56:1350-61. 2010
    ..This is a significantly revised view of the genetic mechanisms underlying synthesis of sulfated and sulfonated STX analogues in toxigenic cyanobacteria...
  12. pmc Sulf-2, a proangiogenic heparan sulfate endosulfatase, is upregulated in breast cancer
    Megumi Morimoto-Tomita
    Department of Anatomy and the UCSF Comprehensive Cancer Center, University of California, San Francisco, 94143 0452, USA
    Neoplasia 7:1001-10. 2005
    ..In support of the possible mechanistic relevance of Sulf-2 upregulation in tumors, purified recombinant Sulf-2 promoted angiogenesis in the chick chorioallantoic membrane assay...
  13. ncbi Molecular and biochemical characterization of two brassinosteroid sulfotransferases from Arabidopsis, AtST4a (At2g14920) and AtST1 (At2g03760)
    Frédéric Marsolais
    Agriculture and Agri Food Canada, Southern Crop Protection and Food Research Centre, 1391 Sandford St, London, ON, N5V 4T3, Canada
    Planta 225:1233-44. 2007
    Mammalian sulfotransferases (EC 2.8.2) are involved in many important facets of steroid hormone activity and metabolism...
  14. pmc The oncogenic effect of sulfatase 2 in human hepatocellular carcinoma is mediated in part by glypican 3-dependent Wnt activation
    Jin Ping Lai
    Miles and Shirley Fiterman Center for Digestive Diseases, Mayo Clinic, Rochester, MN, USA
    Hepatology 52:1680-9. 2010
    ..Opposite effects were observed in SULF2-knockdown models. In vivo, nude mouse xenografts established from SULF2-transfected Hep3B cells showed enhanced GPC3, Wnt3a, and β-catenin levels...
  15. pmc Structural and chemical profiling of the human cytosolic sulfotransferases
    Abdellah Allali-Hassani
    Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada
    PLoS Biol 5:e97. 2007
    ..The data help explain substrate promiscuity in this family and, at the same time, reveal new similarities between hSULT family members that were previously unrecognized by sequence or structure comparison alone...
  16. ncbi Loss of HSulf-1 up-regulates heparin-binding growth factor signaling in cancer
    Jinping Lai
    Mayo Clinic Cancer Center, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA
    J Biol Chem 278:23107-17. 2003
    ..Collectively, these observations provide evidence that HSulf-1 modulates signaling by heparin-binding growth factors, and HSulf-1 down-regulation represents a novel mechanism by which cancer cells can enhance growth factor signaling...
  17. ncbi Glucocorticoids antagonize estrogens by glucocorticoid receptor-mediated activation of estrogen sulfotransferase
    Haibiao Gong
    Center for Pharmacogenetics and Department of Pharmaceutical Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    Cancer Res 68:7386-93. 2008
    ..Because glucocorticoids and estrogens are widely prescribed drugs, our results also urge caution in avoiding glucocorticoid-estrogen interactions in patients...
  18. pmc Expression of estrogenicity genes in a lineage cell culture model of human breast cancer progression
    Jiaqi Fu
    Institute of Environmental Health Sciences, Wayne State University, Detroit, MI, 48201, USA
    Breast Cancer Res Treat 120:35-45. 2010
    ..expression of estrogen receptor (ERalpha and ERbeta) and estrogen metabolism enzymes including cytosolic sulfotransferases (SULT1E1, SULT1A1, SULT2A1, and SULT2B1), steroid sulfatase (STS), aromatase (CYP19), 17beta-hydroxysteroid ..
  19. ncbi Crystal structure of the human estrogen sulfotransferase-PAPS complex: evidence for catalytic role of Ser137 in the sulfuryl transfer reaction
    Lars C Pedersen
    Pharmacogenetics Section, Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 277:17928-32. 2002
    ..have now provided a more complete view of the S(N)2-like in-line displacement reaction catalyzed by sulfotransferases. In the PAPS-bound structure, the side chain nitrogen of the catalytic Lys(47) interacts with the side chain ..
  20. pmc Crystallographic analysis of a hydroxylated polychlorinated biphenyl (OH-PCB) bound to the catalytic estrogen binding site of human estrogen sulfotransferase
    Sergei Shevtsov
    Pharmacogenetic Section, Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Environ Health Perspect 111:884-8. 2003
    ..The crystal structure of hEST with the OH-PCB bound gives physical evidence that certain OH-PCBs can mimic binding of estrogenic compounds in biological systems...
  21. pmc Altered heparan sulfate structure in mice with deleted NDST3 gene function
    Srinivas R Pallerla
    Department of General Zoology and Genetics, Westfalische Wilhelms Universitat Munster, Munster, Germany
    J Biol Chem 283:16885-94. 2008
    ....
  22. pmc Chondroitin-4-sulfation negatively regulates axonal guidance and growth
    Hang Wang
    Developmental Neurobiology Section, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Sci 121:3083-91. 2008
    ..Thus, our findings show that specific sulfation along the carbohydrate backbone carries instructions to regulate neuronal function...
  23. ncbi Sulfation of chondroitin sulfate in human articular cartilage. The effect of age, topographical position, and zone of cartilage on tissue composition
    M T Bayliss
    Royal Veterinary College, Royal College Street, London NW1 OTU, United Kingdom
    J Biol Chem 274:15892-900. 1999
    ..Furthermore, these changes in composition are a consequence of both extracellular, post-translational processing of the core protein of aggrecan and changes in the sulfotransferase activity of the chondrocyte...
  24. ncbi IL-6 and IL-8 increase the expression of glycosyltransferases and sulfotransferases involved in the biosynthesis of sialylated and/or sulfated Lewisx epitopes in the human bronchial mucosa
    Sophie Groux-Degroote
    Unite de Glycobiologie Structurale et Fonctionnelle, UMR CNRS no 8576, Batiment C9, Universite des Sciences et Technologies de Lille, F 59655 Villeneuve d Ascq, Lille, France
    Biochem J 410:213-23. 2008
    ..ST3GAL6 (alpha2,3-sialyltransferase 6 gene) and ST6GAL2 (alpha2,6-sialyltransferase 2 gene)] and GlcNAc-6-O-sulfotransferases [CHST4 (carbohydrate sulfotransferase 4 gene) and CHST6] mRNA...
  25. ncbi Sulfation of thyroid hormone and dopamine during human development: ontogeny of phenol sulfotransferases and arylsulfatase in liver, lung, and brain
    K Richard
    Department of Obstetrics and Gynecology, University of Dundee, Ninewells Hospital and Medical School, Dundee, Scotland, United Kingdom DD1 9SY
    J Clin Endocrinol Metab 86:2734-42. 2001
    ..Here we have investigated the ontogeny of sulfotransferases (SULT) and sulfatase (ARS) involved in the metabolism of thyroid hormone and dopamine...
  26. ncbi Inhibition of sulfotransferases by xenobiotics
    Li Quan Wang
    Department of Medicinal Chemistry, College of Pharmacy, University of Florida, Gainesville, FL 32610, USA
    Curr Drug Metab 7:83-104. 2006
    ..This manuscript will review the literature concerning the inhibition of SULTs by xenobiotics including isoform-selective effects, inhibition kinetics and health effects resulting from the inhibition...
  27. ncbi Identification and characterization of TGFbeta-dependent and -independent cis-regulatory modules in the C4ST-1/CHST11 locus
    C M Willis
    Department of Pediatrics, Integrated Graduate Program, Northwestern University, Chicago, IL, USA
    Genet Mol Res 8:1331-43. 2009
    ..Moreover, we identified TGFbeta- responsive regulatory modules that can function in a cell type-specific fashion. Taken together, our results identify TGFbeta-dependent and -independent cis-regulatory modules of the C4ST-1 gene...
  28. ncbi Tyrosine sulfation: an increasingly recognised post-translational modification of secreted proteins
    Martin J Stone
    Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia
    N Biotechnol 25:299-317. 2009
    ..understanding of protein tyrosine sulfation have come about owing to the cloning of two human tyrosylprotein sulfotransferases (TPST-1 and TPST-2), the development of novel analytical and synthetic methodologies and detailed studies of ..
  29. ncbi Structure, function and polymorphism of human cytosolic sulfotransferases
    Julian Lindsay
    Division of Pharmacy, School of Life Sciences, Queensland University of Technology, Brisbane, Australia
    Curr Drug Metab 9:99-105. 2008
    ..Already research in the field is finding links with cancer and sulfonation-related disease, promising to deliver great advances in clinical practice in the future...
  30. ncbi Nuclear receptors and the regulation of drug-metabolizing enzymes and drug transporters: implications for interindividual variability in response to drugs
    Bradley L Urquhart
    Division of Clinical Pharmacology, London Health Sciences Centre University Hospital, Room ALL 152, 339 Windermere Road, London, Ontario N6A 5A5, Canada
    J Clin Pharmacol 47:566-78. 2007
    ..Finally, they outline how the drug development process has been affected by the current understanding of the involvement of nuclear receptors in the regulation of drug disposition genes...
  31. ncbi Omani-type spondyloepiphyseal dysplasia with cardiac involvement caused by a missense mutation in CHST3
    B Tuysuz
    Department of Pediatric Genetics, Cerrahpasa Medical School, Istanbul University, Istanbul, Turkey
    Clin Genet 75:375-83. 2009
    ..In addition to the previously noted skeletal features, affected members of this family also had cardiac involvement including mitral, tricuspid and/or aortic regurgitations and type E brachydactyly...
  32. ncbi Directed evolution of sulfotransferases and paraoxonases by ancestral libraries
    Uria Alcolombri
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
    J Mol Biol 411:837-53. 2011
    ..We designed and constructed such libraries using serum paraoxonases and cytosolic sulfotransferases (SULTs) as model enzymes...
  33. pmc C4ST-1/CHST11-controlled chondroitin sulfation interferes with oncogenic HRAS signaling in Costello syndrome
    Michael Klüppel
    Department of Pediatrics, Robert H Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60614, USA
    Eur J Hum Genet 20:870-7. 2012
    ....
  34. pmc SULF2 methylation is prognostic for lung cancer survival and increases sensitivity to topoisomerase-I inhibitors via induction of ISG15
    M Tessema
    Lung Cancer Program, Lovelace Respiratory Research Institute, Albuquerque, NM 87108, USA
    Oncogene 31:4107-16. 2012
    ....
  35. pmc WT1-dependent sulfatase expression maintains the normal glomerular filtration barrier
    Valérie A Schumacher
    Department of Medicine, Children s Hospital Boston and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Am Soc Nephrol 22:1286-96. 2011
    ....
  36. ncbi Biosynthesis of the regulatory oxysterol, 5-cholesten-3beta,25-diol 3-sulfate, in hepatocytes
    Xiaobo Li
    Department of Medicine Veterans Affairs McGuire Medical Center, Virginia Commonwealth University, Richmond, VA 23249, USA
    J Lipid Res 48:2587-96. 2007
    ..Specific small interfering RNA decreased SULT2B1b mRNA, protein, and activity levels. These findings demonstrate that mitochondria synthesize 25HC, which is subsequently 3beta-sulfated to form 25HC3S...
  37. ncbi Disturbed expression of phase I and phase II estrogen-metabolizing enzymes in endometrial cancer: lower levels of CYP1B1 and increased expression of S-COMT
    Neli Hevir
    Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia
    Mol Cell Endocrinol 331:158-67. 2011
    ..The importance of the changed ratio between soluble and membrane-bound COMT still needs to be evaluated in further studies...
  38. pmc SULFs in human neoplasia: implication as progression and prognosis factors
    Caroline Bret
    INSERM U847, Institut de Recherche en Biothérapie, CHRU de Montpellier, France
    J Transl Med 9:72. 2011
    ..However, other observations argue for contradictory effects of these sulfatases in cancer, suggesting the complexity of their action in the tumor microenvironment...
  39. pmc Lack of protein-tyrosine sulfation disrupts photoreceptor outer segment morphogenesis, retinal function and retinal anatomy
    David M Sherry
    Department of Cell Biology, University of Oklahoma Health Sciences Center, BMSB 781, 940 Stanton L Young Blvd, Oklahoma City, OK 73104, USA
    Eur J Neurosci 32:1461-72. 2010
    ..sulfation in the retina, we examined retinal function and structure in mice lacking tyrosylprotein sulfotransferases (TPST) 1 and 2...
  40. pmc Human estrogen sulfotransferase (SULT1E1) pharmacogenomics: gene resequencing and functional genomics
    Araba A Adjei
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Medical School Mayo Clinic Mayo Foundation, Rochester, MN 55905, U S A
    Br J Pharmacol 139:1373-82. 2003
    ....
  41. pmc Chondroitin sulfates play a major role in breast cancer metastasis: a role for CSPG4 and CHST11 gene expression in forming surface P-selectin ligands in aggressive breast cancer cells
    Craig A Cooney
    Department of Biochemistry and Molecular Biology, The University of Arkansas for Medical Sciences, Little Rock, 72205, USA
    Breast Cancer Res 13:R58. 2011
    ....
  42. pmc Estrogen sulfotransferase regulates body fat and glucose homeostasis in female mice
    Victor K Khor
    Department of Medicine and Institute for Diabetes, Obesity and Metabolism, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Am J Physiol Endocrinol Metab 299:E657-64. 2010
    ..In contrast, hepatic insulin sensitivity was improved but muscle insulin sensitivity did not change in EST transgenic mice. These results reveal novel effects of EST on adipose tissue and glucose homeostasis in female mice...
  43. ncbi The roles of chondroitin-4-sulfotransferase-1 in development and disease
    Michael Klüppel
    Human Molecular Genetics Program, Children s Memorial Research Center, Department of Pediatrics, Northwestern University, Feinberg School of Medicine, Chicago, Illinois, USA
    Prog Mol Biol Transl Sci 93:113-32. 2010
    ..Proper 4-sulfation of chondroitin by C4ST-1 plays a crucial role in the skeletal development and signaling events, and new evidence is suggestive of a potential role for C4ST-1 in human disease, including cancer...
  44. pmc Distinct functional specificities are associated with protein isoforms encoded by the Drosophila dorsal-ventral patterning gene pipe
    Zhenyu Zhang
    Section of Molecular Cell and Developmental Biology and Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
    Development 136:2779-89. 2009
    ..This suggests the existence of a second mechanism that polarizes the Drosophila embryo, in addition to the ventrally restricted transcription of the pipe gene...
  45. ncbi Expression of multiple chondroitin/dermatan sulfotransferases in the neurogenic regions of the embryonic and adult central nervous system implies that complex chondroitin sulfates have a role in neural stem cell maintenance
    Kaoru Akita
    Department of Cell Morphology and Molecular Neurobiology, Faculty of Biology, Ruhr University Bochum, NDEF 05 594, Universitatsstrasse 150, D 44780 Bochum, Germany
    Stem Cells 26:798-809. 2008
    Chondroitin/dermatan sulfotransferases (C/D-STs) underlie the synthesis of diverse sulfated structures in chondroitin/dermatan sulfate (CS/DS) chains...
  46. ncbi A mutation in Tpst2 encoding tyrosylprotein sulfotransferase causes dwarfism associated with hypothyroidism
    Nobuya Sasaki
    Laboratory of Experimental Animal Science, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060 0818, Japan
    Mol Endocrinol 21:1713-21. 2007
    ..Therefore, we propose that the tyrosine sulfation of TSHR by TPST2 is crucial for TSH signaling and resultant thyroid gland function...
  47. pmc Hsulf-1 regulates growth and invasion of pancreatic cancer cells
    I Abiatari
    Department of General Surgery, University of Heidelberg, Heidelberg, Germany
    J Clin Pathol 59:1052-8. 2006
    ..In vitro overexpression of this enzyme in pancreatic cancer cells decreases responsiveness to fibroblastic growth factor-2, as Hsulf-1 is up regulated in primary pancreatic adenocarcinoma...
  48. pmc Heparin-degrading sulfatases in hepatocellular carcinoma: roles in pathogenesis and therapy targets
    Jin Ping Lai
    Center for Basic Research in Digestive Diseases, Miles and Shirley Fiterman Center for Digestive Diseases, Mayo Clinic College of Medicine and Mayo Clinic Cancer Center, 200 First Street, SW, Rochester, MN 55905, USA
    Future Oncol 4:803-14. 2008
    ..Targeting SULF2 or the interaction between SULF2 and SULF1 may lead to novel therapeutics for the treatment of HCCs...
  49. pmc The tumor suppressor function of human sulfatase 1 (SULF1) in carcinogenesis
    Jin Ping Lai
    Division of Gastroenterology and Hepatology, College of Medicine, Mayo Clinic, Rochester, MN 55905, USA
    J Gastrointest Cancer 39:149-58. 2008
    ..Since sulfated HSPGs serve as co-receptors for many growth factors and cytokines, SULF1 was predicted to modulate growth factor and cytokine signaling...
  50. pmc Anticoagulant heparan sulfate: structural specificity and biosynthesis
    Jian Liu
    Division of Medicinal Chemistry and Natural Products, School of Pharmacy, University of North Carolina, Rm 309, Beard Hall, Chapel Hill, NC 27599, USA
    Appl Microbiol Biotechnol 74:263-72. 2007
    ..These advancements provide the foundation for the development of polysaccharide-based therapeutic agents...
  51. pmc Heparan sulfate 6-O-sulfotransferase 1, a gene involved in extracellular sugar modifications, is mutated in patients with idiopathic hypogonadotrophic hypogonadism
    Janne Tornberg
    Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 108:11524-9. 2011
    ..These findings are consistent with a model in which KAL1 can act as a modulatory coligand with FGF to activate the FGF receptor in an HS-dependent manner...
  52. pmc Sulf-2, a heparan sulfate endosulfatase, promotes human lung carcinogenesis
    H Lemjabbar-Alaoui
    Department of Anatomy, University of California, San Francisco, CA 94143, USA
    Oncogene 29:635-46. 2010
    ..Conversely, forced Sulf-2 expression in non-malignant bronchial epithelial cells produced a partially transformed phenotype. Our findings support an essential role for Sulf-2 in lung cancer, the leading cancer killer...
  53. pmc Heparan sulfate sulfatase SULF2 regulates PDGFRα signaling and growth in human and mouse malignant glioma
    Joanna J Phillips
    Department of Neurological Surgery, UCSF, San Francisco, California 94158, USA
    J Clin Invest 122:911-22. 2012
    ..Our results suggest the bioavailability of growth factors from the microenvironment is a significant contributor to tumor growth in a major subset of human GBM...
  54. ncbi Multiple isoforms of heparan sulfate D-glucosaminyl 3-O-sulfotransferase. Isolation, characterization, and expression of human cdnas and identification of distinct genomic loci
    N W Shworak
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 274:5170-84. 1999
    ..Moreover, the extensive number of 3OST genes with diverse expression patterns of multiple transcripts suggests that the novel 3-OST enzymes, like 3-OST-1, regulate important biologic properties of heparan sulfate proteoglycans...
  55. ncbi Heparan sulfation-dependent fibroblast growth factor signaling maintains embryonic stem cells primed for differentiation in a heterogeneous state
    Fredrik Lanner
    Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
    Stem Cells 28:191-200. 2010
    ..We conclude that N-sulfated HS is required for FGF4 signaling to maintain ES cells primed for differentiation in a heterogeneous state. Inhibiting this pathway facilitates a more naïve ground state...
  56. pmc Expression of a testis-specific form of Gal3st1 (CST), a gene essential for spermatogenesis, is regulated by the CTCF paralogous gene BORIS
    Teruhiko Suzuki
    Laboratory of Immunopathology, National Institutes of Allergy and Infectious Diseases, NIH, Rockville, MD 20852, USA
    Mol Cell Biol 30:2473-84. 2010
    ..Mutation of the BORIS binding site in the promoter reduced the ability of BORIS to activate the promoter. These findings define transcriptional regulation of CST expression as a critical role for BORIS in spermatogenesis...
  57. ncbi Molecular cloning and expression of human and mouse tyrosylprotein sulfotransferase-2 and a tyrosylprotein sulfotransferase homologue in Caenorhabditis elegans
    Y B Ouyang
    Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    J Biol Chem 273:24770-4. 1998
    ..Thus, we have identified two new members of a class of membrane-bound sulfotransferases that catalyze tyrosine O-sulfation...
  58. ncbi HSulf-1 modulates HGF-mediated tumor cell invasion and signaling in head and neck squamous carcinoma
    Jin Ping Lai
    Division of Gastroenterology and Hepatology, Mayo Clinic Cancer Center, Mayo Clinic and Foundation, Rochester, MN 55905, USA
    Oncogene 23:1439-47. 2004
    ....
  59. ncbi The three desulfoglucosinolate sulfotransferase proteins in Arabidopsis have different substrate specificities and are differentially expressed
    Marion Klein
    Institute for Botany, University of Hannover, Germany
    FEBS J 273:122-36. 2006
    b>Sulfotransferases (SOTs) catalyse the transfer of a sulfate group from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to an appropriate hydroxy group of various substrates with the parallel formation of 3'-phosphoadenosine 5'-phosphate...
  60. ncbi Association between sulfotransferase 1A1 genotype and survival of breast cancer patients receiving tamoxifen therapy
    Susan Nowell
    S Nowell, Department of Pharmacology and Toxicology, University of Arkansas for Medical Sciences, Little Rock, USA
    J Natl Cancer Inst 94:1635-40. 2002
    ..We investigated the hypothesis that that high sulfation activity would increase the elimination of 4-OH TAM by examining whether the presence of this polymorphism affects the efficacy of tamoxifen therapy...
  61. ncbi HSulf-1 inhibits angiogenesis and tumorigenesis in vivo
    Keishi Narita
    Department of Experimental Pathology, Mayo Clinic Cancer Center, Rochester, Minnesota 55905, USA
    Cancer Res 66:6025-32. 2006
    ..Collectively, these observations provide the first evidence of a novel mechanism by which HSulf-1 modulates the function of heparan sulfate binding VEGF165 in proliferation and angiogenesis...
  62. ncbi An annotated catalog of salivary gland transcripts from Ixodes scapularis ticks
    Jose M C Ribeiro
    Section of Vector Biology, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Insect Biochem Mol Biol 36:111-29. 2006
    ..Results support the hypothesis that gene duplication, most possibly including genome duplications, is a major player in tick evolution...
  63. pmc Heparan sulfate biosynthesis enzymes EXT1 and EXT2 affect NDST1 expression and heparan sulfate sulfation
    Jenny Presto
    Department of Medical Biochemistry and Microbiology, Biomedical Center, University of Uppsala, Box 582, SE 751 23 Uppsala, Sweden
    Proc Natl Acad Sci U S A 105:4751-6. 2008
    ..Increased NDST activity in fibroblasts with a gene trap mutation in EXT1 supports this notion. These results support a model in which the enzymes of HS biosynthesis form a complex, or a GAGosome...
  64. pmc Antibody GD3G7 selected against embryonic glycosaminoglycans defines chondroitin sulfate-E domains highly up-regulated in ovarian cancer and involved in vascular endothelial growth factor binding
    Gerdy B ten Dam
    Department of Biochemistry 280, Nijmegen Center for Molecular Life Sciences, Radboud University Nijmegen Medical Center, PO Box 9101, 6500 HB Nijmegen, The Netherlands
    Am J Pathol 171:1324-33. 2007
    ..In conclusion, antibody GD3G7 identified rare CS-E-like structures that were strongly expressed in ovarian adenocarcinomas. This antibody might therefore be instrumental for identifying tumor-related CS alterations...
  65. ncbi Dissecting human adrenal androgen production
    William E Rainey
    Department Obstetrics and Gynecology, Division of Reproductive Endocrinology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9032, USA
    Trends Endocrinol Metab 13:234-9. 2002
    ..Insight into the mechanisms that regulate the synthesis of these key components of DHEA(S) synthesis should provide important clues to the regulation of adrenal androgen biosynthesis...
  66. pmc Existence of distinct tyrosylprotein sulfotransferase genes: molecular characterization of tyrosylprotein sulfotransferase-2
    R Beisswanger
    Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany
    Proc Natl Acad Sci U S A 95:11134-9. 1998
    ..A predicted 359-residue type II transmembrane protein in Caenorhabditis elegans with 45% amino acid identity to TPST-2 in a 257-residue segment of the luminal domain points to the evolutionary conservation of the TPST protein family...
  67. ncbi Specificities of three distinct human chondroitin/dermatan N-acetylgalactosamine 4-O-sulfotransferases demonstrated using partially desulfated dermatan sulfate as an acceptor: implication of differential roles in dermatan sulfate biosynthesis
    Tadahisa Mikami
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 278:36115-27. 2003
    ..a high frequency modification of chondroitin sulfate and dermatan sulfate (DS), and three major GalNAc 4-O-sulfotransferases including dermatan 4-O-sulfotransferase-1 (D4ST-1) and chondroitin 4-O-sulfotransferases-1 and -2 (C4ST-1 ..
  68. pmc Tyrosylprotein sulfotransferase: purification and molecular cloning of an enzyme that catalyzes tyrosine O-sulfation, a common posttranslational modification of eukaryotic proteins
    Y B Ouyang
    Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Proc Natl Acad Sci U S A 95:2896-901. 1998
    ..This enzyme defines a new class of Golgi sulfotransferases that may catalyze tyrosine O-sulfation of PSGL-1 and other protein substrates involved in diverse ..
  69. ncbi Epigenetic silencing of HSulf-1 in ovarian cancer:implications in chemoresistance
    J Staub
    Department of Laboratory Medicine and Pathology, Division of Experimental Pathology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Oncogene 26:4969-78. 2007
    ..0146, chi(2) test). Collectively, these data indicate that HSulf-1 is epigenetically silenced in ovarian cancer and that epigenetic therapy targeting HSulf-1 might sensitize ovarian tumors to conventional first-line therapies...
  70. pmc Cholesterol metabolite, 5-cholesten-3β-25-diol-3-sulfate, promotes hepatic proliferation in mice
    Xin Zhang
    Department of Medicine, Virginia Commonwealth University Veterans Affairs McGuire Medical Center, 1201 Broad Rock Boulevard, Richmond, VA 23249, United States
    J Steroid Biochem Mol Biol 132:262-70. 2012
    ..Synthetic LXR agonist T0901317 effectively blocked 25HC3S-induced hepatic proliferation...
  71. ncbi Up-regulated expression of sulfatases (SULF1 and SULF2) as prognostic and metastasis predictive markers in human gastric cancer
    Keun Hur
    Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea
    J Pathol 228:88-98. 2012
    ..We provide novel evidence that hypomethylation of promoter CpG islands within SULF genes imparts them with oncogenic potential in GC. Moreover, our data suggest that SULF1 may serve as a promising biomarker for patients with GC...
  72. pmc Enzyme interactions in heparan sulfate biosynthesis: uronosyl 5-epimerase and 2-O-sulfotransferase interact in vivo
    M A Pinhal
    Department of Cellular and Molecular Medicine, Glycobiology Research and Training Center, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0687, USA
    Proc Natl Acad Sci U S A 98:12984-9. 2001
    ..network by the concerted action of several glycosyltransferases, an epimerase, and multiple sulfotransferases. In this report, we have examined the location and interaction of tagged forms of five of the biosynthetic ..
  73. pmc Specific genes involved in synthesis and editing of heparan sulfate proteoglycans show altered expression patterns in breast cancer
    Iván Fernández-Vega
    Department of Pathology, Hospital Universitario Central de Asturias, Oviedo 33006, Spain
    BMC Cancer 13:24. 2013
    ..The purpose of this study is to increase knowledge of HSPG alterations in breast cancer...
  74. pmc Two dermatan sulfate epimerases form iduronic acid domains in dermatan sulfate
    Benny Pacheco
    Department of Experimental Medical Science, Biomedical Center D12, Lund University, Tornavagen 10, SE 221 84 Lund, Sweden
    J Biol Chem 284:9788-95. 2009
    ..DS-epi2 has been genetically linked to bipolar disorder, which suggests that the dermatan sulfate domains generated by a defective enzyme may be involved in the etiology of the disease...
  75. pmc Congenital joint dislocations caused by carbohydrate sulfotransferase 3 deficiency in recessive Larsen syndrome and humero-spinal dysostosis
    Pia Hermanns
    Center for Pediatrics and Adolescent Medicine, University of Freiburg, 79106 Freiburg, Germany
    Am J Hum Genet 82:1368-74. 2008
    ..These observations provide a molecular basis for recessive Larsen syndrome and indicate that recessive Larsen syndrome, humero-spinal dysostosis, and spondyloepiphyseal dysplasia Omani type form a phenotypic spectrum...
  76. ncbi Inactivating PAPSS2 mutations in a patient with premature pubarche
    Cees Noordam
    Department of Pediatrics Metabolic and Endocrine Disorders, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    N Engl J Med 360:2310-8. 2009
    ..In vitro coincubation of human SULT2A1 and wild-type or mutant PAPSS2 proteins confirmed the inactivating nature of the mutations. These observations indicate that PAPSS2 deficiency is a monogenic adrenocortical cause of androgen excess...
  77. ncbi Structural analysis of glycosaminoglycans in animals bearing mutations in sugarless, sulfateless, and tout-velu. Drosophila homologues of vertebrate genes encoding glycosaminoglycan biosynthetic enzymes
    H Toyoda
    Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, USA
    J Biol Chem 275:21856-61. 2000
    ..Defects in sulfateless, a gene encoding a protein with similarity to vertebrate N-deacetylase/N-sulfotransferases, do not affect chondroitin sulfate levels or composition but dramatically alter the composition of heparin ..
  78. ncbi N-Acetylglucosamine 6-O-sulfotransferase-1 is required for brain keratan sulfate biosynthesis and glial scar formation after brain injury
    Haoqian Zhang
    Department of Biochemistry, Institute for Advanced Research, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8601, Japan
    Glycobiology 16:702-10. 2006
    ..These findings highlight the indispensable role of GlcNAc6ST-1 in brain KS biosynthesis and glial scar formation after brain injury...
  79. pmc Sulfation of 25-hydroxycholesterol by SULT2B1b decreases cellular lipids via the LXR/SREBP-1c signaling pathway in human aortic endothelial cells
    Qianming Bai
    Department of Medicine, Virginia Commonwealth University Veterans Affairs McGuire Medical Center, Richmond, VA 23249, United States
    Atherosclerosis 214:350-6. 2011
    ..We evaluate the effect of 25HC sulfation on lipid metabolism by overexpressing the gene encoding SULT2B1b in human aortic endothelial cells (HAECs) in culture...
  80. pmc Normal levels of anticoagulant heparan sulfate are not essential for normal hemostasis
    Sassan HajMohammadi
    Section of Cardiology, Department of Medicine, Dartmouth Medical School, Hanover, New Hampshire, USA
    J Clin Invest 111:989-99. 2003
    ..Instead, 3-OST-1-deficient mice exhibited unanticipated phenotypes suggesting that HS(act) or additional 3-OST-1-derived structures may serve alternate biologic roles...
  81. ncbi Loss-of-function mutations of CHST14 in a new type of Ehlers-Danlos syndrome
    Noriko Miyake
    Department of Human Genetics, Yokohama City University Graduate School of Medicine, Yokohama 236 0004, Japan
    Hum Mutat 31:966-74. 2010
    ..These findings indicate the important role of decorin DS in the extracellular matrix and a novel pathomechanism in EDS...
  82. ncbi Potential tumor markers for human gastric cancer: an elevation of glycan:sulfotransferases and a concomitant loss of alpha1,2-fucosyltransferase activities
    E V Chandrasekaran
    Department of Cancer Biology, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA
    J Cancer Res Clin Oncol 133:599-611. 2007
    ..The present study was aimed to identify the carbohydrate associated transferases which exhibit the most marked and consistent change of activity in gastric tumorigenesis...
  83. ncbi Differential enzymatic characteristics and tissue-specific expression of human TPST-1 and TPST-2
    Emi Mishiro
    Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki 889 2192
    J Biochem 140:731-7. 2006
    ..These latter findings may imply distinct physiological functions of TPST-1 and TPST-2...
  84. pmc Portable sulphotransferase domain determines sequence specificity of heparan sulphate 3-O-sulphotransferases
    T Yabe
    Angiogenesis Research Center, Department of Medicine, Harvard Medical School, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    Biochem J 359:235-41. 2001
    ..Thus sequence-specific properties of 3-OSTs are defined by a self-contained sulphotransferase domain and are not directly influenced by the divergent N-terminal region...
  85. pmc Targeted disruption of tyrosylprotein sulfotransferase-2, an enzyme that catalyzes post-translational protein tyrosine O-sulfation, causes male infertility
    Atefeh Borghei
    Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA
    J Biol Chem 281:9423-31. 2006
    Tyrosine O-sulfation is a post-translational modification mediated by one of two Golgi tyrosylprotein sulfotransferases (TPST-1 and -2) expressed in all mammalian cells...
  86. ncbi Chondroitin sulfate/dermatan sulfate hybrid chains in the development of cerebellum. Spatiotemporal regulation of the expression of critical disulfated disaccharides by specific sulfotransferases
    Chie Mitsunaga
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 281:18942-52. 2006
    ..of the brain, in situ hybridization was performed to examine expression of three CS/DS GalNAc 4-O-sulfotransferases, D4ST-1, C4ST-1, and C4ST-2, and a single uronyl 2-O-sulfotransferase (UST) involved in the biosynthesis of ..
  87. pmc Polymorphisms in the SULF1 gene are associated with early age of onset and survival of ovarian cancer
    Chan H Han
    Department of Epidemiology, The University of Texas M, d, Anderson Cancer Center, Houston, TX 77030, USA
    J Exp Clin Cancer Res 30:5. 2011
    ..SULF1 expression has been reported to be decreased in various cancers, including ovarian cancer. We hypothesized that single nucleotide polymorphisms (SNPs) of SULF1 would impact clinicopathologic characteristics...
  88. ncbi Sulfation pharmacogenetics: SULT1A1 and SULT1A2 allele frequencies in Caucasian, Chinese and African-American subjects
    E J Carlini
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Medical School Mayo Graduate School Mayo Clinic, Rochester, MN 55905, USA
    Pharmacogenetics 11:57-68. 2001
    ..001). These observations represent a step towards determining the possible functional implications for individual variations in sulfate conjugation of common genetic polymorphisms for SULT1A1 and SULT1A2...
  89. ncbi Mechanisms of Gurken-dependent pipe regulation and the robustness of dorsoventral patterning in Drosophila
    Francesca Peri
    Institut fur Entwicklungsbiologie, Universitat zu Koln, Gyrhofstr 17, D 50923 Köln, Germany
    Development 129:2965-75. 2002
    ..This indicates that potent inhibitory processes prevent pipe dependent Toll activation at the dorsal side of the egg...
  90. ncbi Reverse geometrical selectivity in glucuronidation and sulfation of cis- and trans-4-hydroxytamoxifens by human liver UDP-glucuronosyltransferases and sulfotransferases
    Takahito Nishiyama
    Department of Drug Metabolism and Molecular Toxicology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji shi, Tokyo 192 0392, Japan
    Biochem Pharmacol 63:1817-30. 2002
    ..UGTs1A3 and 1A6 had no detectable activity toward these substrates. Among the four known major sulfotransferases (SULTs) occurring in the human liver, SULT1A1 was strongly suggested to play the most important role in the ..
  91. pmc Obesity-induced diabetes (diabesity) in C57BL/KsJ mice produces aberrant trans-regulation of sex steroid sulfotransferase genes
    E H Leiter
    Jackson Laboratory, Bar Harbor, Maine 04609
    J Clin Invest 93:2007-13. 1994
    ..These db gene-elicited effects were specific for the sex steroid sulfotransferases since other potential sex steroid metabolizing enzymes (phenol sulfotransferase, sex steroid sulfohydrolase, ..
  92. pmc Sulfolipids and glycolipid sulfotransferase activities in human renal cell carcinoma cells
    T Kobayashi
    Biochemistry Laboratory, Hokkaido University School of Medicine, Sapporo, Japan
    Br J Cancer 67:76-80. 1993
    ..established from human renal cell carcinoma was characterized for the presence of sulfolipids and glycolipid sulfotransferases. Sulfolipids were found to constitute a large part of the acidic glycolipid fraction in SMKT-R3 cells...
  93. ncbi Sulfotransferases and sulfated oligosaccharides
    Koichi Honke
    Department of Biochemistry, Osaka University Medical School, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Med Res Rev 22:637-54. 2002
    ..The sulfation of oligosaccharides is catalyzed by the Golgi-associated sulfotransferases. Recent success in molecular cloning of these sulfotransferases has brought a breakthrough in the ..
  94. ncbi HSulf-1 inhibits cell proliferation and invasion in human gastric cancer
    Jie Li
    School of Life Sciences, Tsinghua University, Beijing, China
    Cancer Sci 102:1815-21. 2011
    ..Together, the results of the present study suggest that extracellular HSulf-1 may function as a negative regulator of proliferation and invasion in gastric cancer by suppressing Wnt ⁄ β-catenin signaling at the cell surface...
  95. ncbi Spatially restricted expression of pipe in the Drosophila egg chamber defines embryonic dorsal-ventral polarity
    J Sen
    Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Cell 95:471-81. 1998
    ..Thus, the localized expression of pipe and the spatially restricted modification of carbohydrate chains play pivotal roles in the mechanisms that establish embryonic pattern and integrate follicular and embryonic polarity...
  96. ncbi Crystal structure of the sulfotransferase domain of human heparan sulfate N-deacetylase/ N-sulfotransferase 1
    Y Kakuta
    Pharmacogenetics Section, Laboratory of Reproductive and Developmental Toxicology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 274:10673-6. 1999
    ..The conserved residue Lys-614 is in position to form a hydrogen bond with the bridge oxygen of the 5'-phosphate...
  97. ncbi Regulated translation of heparan sulfate N-acetylglucosamine N-deacetylase/n-sulfotransferase isozymes by structured 5'-untranslated regions and internal ribosome entry sites
    Kay Grobe
    Department of Cellular and Molecular Medicine, Glycobiology Training and Research Center, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0687, USA
    J Biol Chem 277:30699-706. 2002
    ..region (5'-UTR) sequences of the four vertebrate heparan sulfate/heparin GlcNAc N-deacetylase/N-sulfotransferases (NDSTs) and their role in translational regulation in vivo and in vitro...
  98. ncbi Drosophila heparan sulfate 6-O-sulfotransferase (dHS6ST) gene. Structure, expression, and function in the formation of the tracheal system
    K Kamimura
    Department of Biology, Tokyo Metropolitan University, Hachioji shi, Tokyo 192 0397, Japan
    J Biol Chem 276:17014-21. 2001
    ..Sulfation at each different position of heparan sulfate is catalyzed by distinct enzymes, sulfotransferases. In this study, we identified and characterized Drosophila heparan sulfate 6-O-sulfotransferase (dHS6ST)...
  99. ncbi Reduced body weight and increased postimplantation fetal death in tyrosylprotein sulfotransferase-1-deficient mice
    Ying Bin Ouyang
    Cardiovascular Biology, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA
    J Biol Chem 277:23781-7. 2002
    Tyrosine sulfation is mediated by one of two Golgi isoenzymes, called tyrosylprotein sulfotransferases (TPST-1 and TPST-2)...
  100. pmc Ablation of GalNAc-4-sulfotransferase-1 enhances reproduction by altering the carbohydrate structures of luteinizing hormone in mice
    Yiling Mi
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Clin Invest 118:1815-24. 2008
    ..Thus, sulfate modification of the terminal glycosylation of LH plays a central role in regulating the hypothalamic-pituitary-gonad axis in vivo...
  101. ncbi Heparan sulfate 3-O-sulfotransferase isoform 5 generates both an antithrombin-binding site and an entry receptor for herpes simplex virus, type 1
    Guoqing Xia
    Cell and Molecular Biology, Biomedical Center C13, Lund University, Lund S 22184, Sweden
    J Biol Chem 277:37912-9. 2002
    ..These results provide a new insight regarding the mechanism for the biosynthesis of biologically active HS...

Research Grants64

  1. Novel diagnostic and safety biomarkers of liver injury and hepatotoxicity
    Stanislav I Svetlov; Fiscal Year: 2010
    ..biomarkers in rat models of hepatic injury with the argininosuccinate synthase (ASS) and hepatic estrogen sulfotransferases (EST-1) being the most sensitive markers which rapidly accumulated in blood and correlated with the severity ..
  2. Exploratory Studies on the Anti-Breast Cancer Function of Bamboo Extract
    Jun Panee; Fiscal Year: 2010
    ..BEX supplementation also enhanced the activity of sulfotransferases (SULT) in the liver, an enzyme family that conjugates estrogens...
  3. Regulation of Sulfotransferases by LXR and its Implication in Pathophysiology
    Wen Xie; Fiscal Year: 2011
    ..The most notable is the induction of the Phase II sulfotransferases (SULTs) in the VP-LXRa mice and LXR agonist-treated wild type mice...
  4. Retinal degeneration caused by alterations in protein tyrosine O-sulfation
    Muayyad R Al-Ubaidi; Fiscal Year: 2013
    ..conditional knockout mice for both of the enzymes responsible for protein sulfation, protein tyrosyl sulfotransferases 1 and 2...
  5. GLYCOPROTEIN HORMONE OLIGOSACCHARIDES
    Jacques U Baenziger; Fiscal Year: 2010
    ..We will define the impact of genetic ablation of the GalNAc-4- sulfotransferases and the protein-selective betaGTs on LH function...
  6. Disposition of Flavonoids via Metabolic Interplay
    Ming Hu; Fiscal Year: 2012
    ..Our previous research has shown that key components of flavonoid disposition are conjugating enzymes (i.e., sulfotransferases and UDP-glucuronosyltransferases) and efflux transporters (e.g., BCRP and MRP2)...
  7. Carbohydrate Antigenic Biomarkers for Epithelial Cancers
    Minoru Fukuda; Fiscal Year: 2013
    ..The second is cell transfection of specific glycosyltransferases and other glyco-modifying enzymes such as sulfotransferases, an approach that has recently met with considerable success...
  8. Identification of Tyrosine-Sulfated Proteins in the Male Genital Tract
    Kevin L Moore; Fiscal Year: 2012
    Protein-tyrosine sulfation is mediated by one of only two Golgi tyrosylprotein sulfotransferases in the human and mouse genome (TPST-1 and TPST-2) that sulfate an unknown number of secreted and transmembrane proteins that transit the ..
  9. Acetaminophen Pharmacogenetics
    Michael H Court; Fiscal Year: 2010
    ..that have been identified as critical to these pathways, including the UDP-glucuronosyltransferases (UGTs), sulfotransferases (SULTs), and cytochromes P450 (CYPs)...
  10. Structure and Function of 3-O-sulfation in Heparan Sulfate
    Jeffrey D Esko; Fiscal Year: 2013
    ..A family of seven glucosaminyl 3-O-sulfotransferases (Hs3st) exists in vertebrates...
  11. HUMAN CYTOSOLIC SULFOTRANSFERASES
    Charles N Falany; Fiscal Year: 2013
    ..We propose to investigate the structural changes and interactions as well as the mechanistic features associated with substrate selectivity and kinetics in SULT2A1 both in vitro and in intact cultured cells. ..
  12. Ontogeny of the Phase II cytosolic sulfotransferases and adverse drug reactions
    Ming Cheh Liu; Fiscal Year: 2013
    ....
  13. Functions of Heparan Sulfate Proteoglycans in Axon Guidance and Degeneration.
    FABIENNE POULAIN; Fiscal Year: 2013
    ..It will test te roles of the 6-O and 3-O sulfotransferases that modify HS chains with sulfations at specific positions, and examine how synthetic HS oligosaccharides ..
  14. Nuclear Receptor Regulation of Bile Acid Sulfation
    ANDREW JOSEPH LICKTEIG; Fiscal Year: 2012
    ..The objective here, which is my first step in pursuit of that goal, is to determine which cytosolic sulfotransferases (Sults) are pharmacologically inducible and responsible for BA-sulfation...
  15. Carbohydrate Receptors, Preterm Labor, and Periodontitis
    Wenge Ma; Fiscal Year: 2009
    ..mouse salivary and uterine mucin oligosaccharide sulfation by profiling expression of the relevant glycosyl sulfotransferases. The information obtained from these studies will form a sound foundation to further study the adhesive ..
  16. Targeting L-Selectin in Chronic Murine Ileitis
    Jesus Rivera-Nieves; Fiscal Year: 2013
    ..Unexpectedly, TNF?ARE mice that lack L-selectin or the corresponding sulfotransferases responsible for its functional ligands, develop greatly attenuated disease...
  17. HEPATOBILIARY DISPOSITION IN TOXICOLOGY
    Curtis Klaassen; Fiscal Year: 2003
    ..Both rats and humans are known to have seven major sulfotransferases. Rats have four phenol-sulfotransferases and three hydroxysteroid sulfotransferases, whereas humans have six ..
  18. Synthetic Heparan Sulfate: Probing Biosynthesis to Prepare Defined Drugs
    Jian Liu; Fiscal Year: 2013
    ..These HS polymers will then be modified by biosynthetic enzymes, including O-sulfotransferases, and C5-epimerase and/or catabolic enzymes including endo-6- Oendosulfatases and heparanase...
  19. Uncovering the controlling mechanisms in heparan sulfate biosynthesis
    Jian Liu; Fiscal Year: 2013
    ..HS biosynthesis involves a series of specialized HS sulfotransferases, glycosyltransferases and an epimerase...
  20. MOLECULAR STUDIES OF CORNEAL TRANSPARENCY
    John Hassell; Fiscal Year: 2003
    ..The third aim is to characterize the expression and routing of the KS sulfotransferases that regulate the maturation of the KS containing proteoglycans by preparing antibodies to the ..
  21. Effect of Cirrhosis and Shunts on Drug Disposition
    Stephen Hall; Fiscal Year: 2006
    ..Finally, the expression of other enzymes such as UDP-glucuronosyltransferases (UGTs), sulfotransferases (SULTs) and p-glycoprotein may also be altered in cirrhosis...
  22. FLAVONOIDS BLOCK SULFATION-INDUCED CARCINOGEN ACTIVATION
    Thomas Walle; Fiscal Year: 1999
    ..The hypothesis is that flavonoids in plant food and beverages are potent inhibitors of human sulfotransferases (STs), enzymes known to catalyze the formation of reactive sulfuric acid esters of many common carcinogens, ..
  23. ACTIVE SITE STUDIES OF HUMAN SULFOTRANSFERASES
    Guangping Chen; Fiscal Year: 2003
    ..The characterization of the active sites of STs will also help to find mechanism-based specific inhibitors/activators for use in studying the physiological functions of STs and designing new drugs. ..
  24. RECEPTOR TOPOGRAPHY IN MICROVASCULAR LEUKOCYTE ADHESION
    Ulrich von Andrian; Fiscal Year: 2005
    ..topography of lymphocyte traffic molecules involved in L-selectin-mediated rolling (glycosyltransferases and sulfotransferases, subaim 1.1) and subsequent integrin-dependent arrest (ICAM-l and -2)...
  25. MEMBRANE TOPOLOGY AND BIOSYNTHESIS OF GLYCOSAMINOGLYCANS
    CARLOS HIRSCHBERG; Fiscal Year: 2002
    ..e) Continue with our efforts to clone and express O-sulfotransferases by probable sequence homology with the N-sulfotransferases, using trachea and aorta muscle cells, which have ..
  26. SULFOTRANSFERASE INACTIVATION BY CARCINOGENS
    BONNIE MANGOLD; Fiscal Year: 1990
    The sulfotransferases are enzymes which catalyze the sulfation of the hydroxyl functionalities of a wide variety of exogenous and endogenous compounds...
  27. Tyrosine Sulfation and Chemokine Receptor Function
    Kevin Moore; Fiscal Year: 2006
    Tyrosine sulfation is a post-translational modification mediated by one of two Golgi tyrosylprotein sulfotransferases (TPST-1 and TPST-2), that catalyze the transfer of sulfate from PAPS to lumenally oriented tyrosine residues in ..
  28. BIOCHEMISTRY OF NORMAL AND DYSTROPHIC CORNEAS
    Jules Baum; Fiscal Year: 1991
    ..of keratan sulfate (KS) proteoglycan in corneas with macular dystrophy is due to the deficiency of KS-sulfotransferases in the diseased corneas...
  29. Genetic Variation in Estrogen Sulfation Genes and Endometrial Cancer Risk
    VERONICA SETIAWAN; Fiscal Year: 2009
    ..Sulfate conjugation plays an important role in the biotransformation of estrogens. Estrogen sulfotransferases catalyze the conversion of biologically active estrogens to inactive estrogen sulfates, thereby "diverting" ..
  30. GLYCOPROTEIN HORMONE OLIGOSACCHARIDES
    JACQUES BAENZIGER; Fiscal Year: 2006
    ..3) define the structure:function relationships of the GalNAc-4-sulfotransferases. And 4) Clone the protein-specific Beta 1,4-GalNAc-transferase and characterize its peptide recognition ..
  31. Pharmacogenetics of Sulfate Conjugation
    VIRGIL JORDAN; Fiscal Year: 2006
    ..Sulfation reactions are catalyzed by cytosolic sulfotransferases (SULTs)...
  32. TYROSYLPROTEIN SULFOTRANSFERASES
    Kevin Moore; Fiscal Year: 2002
    ..We recently identified and cloned a family of two mammalian tyrosylprotein sulfotransferases, designated TPST-1 and TPST-2...
  33. Fluorescent HTS Assays for Human Sulfotransferases
    Robert Lowery; Fiscal Year: 2006
    ..In addition a full panel of 20 human sulfotransferases - including several important genetic variants - will be expressed, and purified in parallel using automated,..
  34. Modulation of steroid sulfation by celecoxib-like drugs
    Margaret James; Fiscal Year: 2009
    ..As well as inhibiting COX-2, celecoxib interacts with sulfotransferases (SULT)...
  35. STRUCTURE AND FUNCTION OF SYNTHETIC GLYCOSAMINOGLYCANS
    PETER SEEBERGER; Fiscal Year: 2004
    ..as part of the overall program will be used as molecular tools to elucidate the substrate specificity of 3-0-sulfotransferases, which are responsible for creating the fine structure of heparin...
  36. MAMMARY CARCINOGENESIS BY N-SUBSTITUTED ARYL COMPOUNDS
    Danuta Malejka Giganti; Fiscal Year: 2000
    ..in Salmonella typhimurium and to investigate the bioactivation of C9-oxidized fluorenes by rat liver sulfotransferases. 4) To identify DNA adducts formed in vivo from the bioactivation of nitrofluorenes, C9-oxidized fluorenes, ..
  37. Mass Spectrometry and Enzyme Processes
    JULIE ANN LEARY; Fiscal Year: 2010
    ..2) Kinetics, substrate determination, and mechanism determination of tyrosylprotein sulfotransferases. This will also include a new procedure for unambiguous determination of sites of sulfation on natural ..
  38. CHARACTERIZATION OF HUMAN BRAIN SULFOTRANSFERASES
    JEROME ROTH; Fiscal Year: 1990
    ..There are a number of substrate specific sulfotransferases that are responsible for conjugation of these endogenous biologically active substances and as previously ..
  39. MECHANISMS OF SULFATE TRANSPORT AND REGULATION
    Michael Jennings; Fiscal Year: 2007
    ..These experiments will provide new insights into the regulation of a basic pathway (SO4= supply to sulfotransferases) that is of importance not only in the skeletal system but also in many branches of human pathophysiology...
  40. Fluorescent HTS Assays for Human Sulfotransferases
    Robert Lowery; Fiscal Year: 2003
    ..hampered a lack of high throughput screening assays for delineating the specificity of the 11 known human sulfotransferases for endogenous hormones and drug candidates...
  41. Oriented Protein Microarray for Functional Studies
    Athena Guo; Fiscal Year: 2005
    ..The latter was demonstrated by the observation that enzymatic activity of oriented sulfotransferases on the surface was the same as that of solution phase enzymes, while randomly oriented enzymes on the ..
  42. BIOCHEMISTRY OF GASTRIC SULFATED GLYCOPROTEINS
    Martin Horowitz; Fiscal Year: 1980
    b>Sulfotransferases which transfer sulfate from 3'-phosphoadenylylsulfate to glycoprotein have been demonstrated in the rat surface and sub-surface or deep mucosa...
  43. Role of 3-0-sulfotransferases in HSV entry
    Deepak Shukla; Fiscal Year: 2003
    ..abstract not supplied ..
  44. Conference: Molecular Mechanism of Leukocyte Trafficking
    Steven Rosen; Fiscal Year: 2002
    ..The resulting interactions are likely to stimulate new approaches to the critical remaining problems in trafficking research. ..
  45. CELL SURFACE LECTINS AND INTERCELLULAR ADHESION
    Steven Rosen; Fiscal Year: 2009
    ..progress has been made towards the molecular characterization of these ligands and the identification of the sulfotransferases involved in their synthesis...
  46. Mechanism of protection of DAF and CD59 in SLE
    Wenchao Song; Fiscal Year: 2009
    ..Such results should facilitate the development of innovative therapeutic strategies for human SLE. ..
  47. Role of Heparan Sulfate-Degrading Sulfatases in Pancreatic Adenocarcinomas
    Steven Rosen; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  48. ARYL AND ALCOHOL SULFOTRANSFERASES IN DRUG METABOLISM
    Michael W Duffel; Fiscal Year: 2010
    The roles of sulfotransferases in detoxication of many drugs, as well as in the metabolic activation of many chemical carcinogens, are increasingly recognized as highly significant...
  49. Molecular Pharmacology of Estrogen Sulfotransferase
    Wenchao Song; Fiscal Year: 2006
    ..has been learned on the biochemistry, structural biology and pharmacogenetics of EST and related steroid sulfotransferases, the molecular pharmacology and in vivo function of EST and other steroid sulfotransferases remain ..
  50. Gel Depot for Narcotic Addiction
    Chinnaswamy Kasinathan; Fiscal Year: 2006
    ..We strongly believe that studies proposed here will lead to the development of simple but effective therapy in the treatment of opiate addiction, which still remains a major health and societal problem around the world. ..
  51. ETIOLOGIC HETEROGENEITY IN LATE ONSET BREAST CANCER
    Timothy Rebbeck; Fiscal Year: 2001
    ..The results of these analyses will be used to attempt to elucidate the complex, multifactorial aetiology of late onset breast cancer. ..
  52. HOST GENES AND COLONIC BACTERIA--PROBING FOR INHERITANCE
    Johanna Lampe; Fiscal Year: 2002
    ..If we can establish that the equol-producer phenotype is an inherited trait, we can then work to identify the associated genotype and determine more effectively whether this is a biomarker of breast cancer risk. ..
  53. Pharmacogenetic Epidemiology of Birth Defects and Cancer
    Timothy Rebbeck; Fiscal Year: 2004
    ....
  54. Heparin like Glycosaminoglycan Oligosaccharides
    Ram Sasisekharan; Fiscal Year: 2004
    ..Similar technologies for DNA and proteins include things like gene chips that have revolutionized the study of these molecules. We hope advancements, like out technology, will do the same thing for HLGAGs. ..
  55. ETHANOL EFFECTS ON BONE FORMATION IN PREGNANCY
    Martin Ronis; Fiscal Year: 2006
    ..The role of CYP2E1 and free radicals in the skeletal toxicity of ethanol during these periods will also be studied. ..
  56. Mechanisms and Functions of Human Sulfotransferases
    Guangping Chen; Fiscal Year: 2009
    Phase II drug metabolizing enzymes sulfotransferases (SULTs) catalyzed sulfation is important in the regulation of different hormones and the detoxification of drugs and other xenobiotics...
  57. Coordinate Regulation of Uptake and Efflux Transporters
    Curtis D Klaassen; Fiscal Year: 2010
    ....
  58. Glycoside Inhibitors of Tumor Metastasis
    Jeffrey Esko; Fiscal Year: 2009
    ..Solid tumor formation and inflammatory reactions will be analyzed to confirm the selective inhibitory action of the analog on tumor metastasis. ..
  59. INTL CONG OF VIROLOGY, SAN FRANCISCO, ASV TRAVEL REQUEST
    PATRICIA SPEAR; Fiscal Year: 2005
    ....