protein kinase c

Summary

Summary: An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.

Top Publications

  1. ncbi Biochemistry and molecular cell biology of diabetic complications
    M Brownlee
    Department of Medicine, Diabetes Research and Training Center, Albert Einstein College of Medicine, Bronx, New York, 10461, USA
    Nature 414:813-20. 2001
  2. pmc Protein kinase C-theta mediates negative feedback on regulatory T cell function
    Alexandra Zanin-Zhorov
    Molecular Pathogenesis Program, Helen and Martin Kimmel Center for Biology and Medicine, Skirball Institute of Biomolecular Medicine, Department of Pathology, New York University School of Medicine, New York, NY 10016, USA
    Science 328:372-6. 2010
  3. pmc PKM-ζ is not required for hippocampal synaptic plasticity, learning and memory
    Lenora J Volk
    Department of Neuroscience, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Nature 493:420-3. 2013
  4. ncbi Wnt5a signaling directly affects cell motility and invasion of metastatic melanoma
    Ashani T Weeraratna
    National Human Genome Research Institute, Cancer Genetics Branch, National Institutes of Health, Bethesda, MD 20892, USA
    Cancer Cell 1:279-88. 2002
  5. ncbi PKC-theta is required for TCR-induced NF-kappaB activation in mature but not immature T lymphocytes
    Z Sun
    Molecular Pathogenesis Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York 10016, USA
    Nature 404:402-7. 2000
  6. pmc Structural basis of protein kinase C isoform function
    Susan F Steinberg
    Department of Pharmacology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    Physiol Rev 88:1341-78. 2008
  7. ncbi Lipid-induced insulin resistance in human muscle is associated with changes in diacylglycerol, protein kinase C, and IkappaB-alpha
    Samar I Itani
    Diabetes Unit, Section of Endocrinology, Department of Medicine, Boston University Medical Center, Boston, MA, USA
    Diabetes 51:2005-11. 2002
  8. ncbi Mechanism of hepatic insulin resistance in non-alcoholic fatty liver disease
    Varman T Samuel
    Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    J Biol Chem 279:32345-53. 2004
  9. pmc Prkcz null mice show normal learning and memory
    Anna M Lee
    Ernest Gallo Clinic and Research Center, Department of Neurology, University of California, San Francisco, 5858 Horton Street, Suite 200, Emeryville, California 94608, USA
    Nature 493:416-9. 2013
  10. ncbi High glucose level and free fatty acid stimulate reactive oxygen species production through protein kinase C--dependent activation of NAD(P)H oxidase in cultured vascular cells
    T Inoguchi
    Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushyu University, Fukuoka, Japan
    Diabetes 49:1939-45. 2000

Detail Information

Publications409 found, 100 shown here

  1. ncbi Biochemistry and molecular cell biology of diabetic complications
    M Brownlee
    Department of Medicine, Diabetes Research and Training Center, Albert Einstein College of Medicine, Bronx, New York, 10461, USA
    Nature 414:813-20. 2001
    ..This integrating paradigm provides a new conceptual framework for future research and drug discovery...
  2. pmc Protein kinase C-theta mediates negative feedback on regulatory T cell function
    Alexandra Zanin-Zhorov
    Molecular Pathogenesis Program, Helen and Martin Kimmel Center for Biology and Medicine, Skirball Institute of Biomolecular Medicine, Department of Pathology, New York University School of Medicine, New York, NY 10016, USA
    Science 328:372-6. 2010
    ..b>Protein kinase C-theta (PKC-theta) recruitment to the immunological synapse is required for full Teff activation...
  3. pmc PKM-ζ is not required for hippocampal synaptic plasticity, learning and memory
    Lenora J Volk
    Department of Neuroscience, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Nature 493:420-3. 2013
    ..Sustained activation of the brain-specific protein kinase C (PKC) isoform protein kinase M-ζ (PKM-ζ) has been reported to be necessary for both LTP maintenance and ..
  4. ncbi Wnt5a signaling directly affects cell motility and invasion of metastatic melanoma
    Ashani T Weeraratna
    National Human Genome Research Institute, Cancer Genetics Branch, National Institutes of Health, Bethesda, MD 20892, USA
    Cancer Cell 1:279-88. 2002
    ..Furthermore, Wnt5a expression in human melanoma biopsies directly correlated to increasing tumor grade. These observations support a role for Wnt5a in human melanoma progression...
  5. ncbi PKC-theta is required for TCR-induced NF-kappaB activation in mature but not immature T lymphocytes
    Z Sun
    Molecular Pathogenesis Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York 10016, USA
    Nature 404:402-7. 2000
    ..expression requires the activation of one or more of the multiple isoenzymes of serine/threonine-specific protein kinase C (PKC)...
  6. pmc Structural basis of protein kinase C isoform function
    Susan F Steinberg
    Department of Pharmacology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    Physiol Rev 88:1341-78. 2008
    b>Protein kinase C (PKC) isoforms comprise a family of lipid-activated enzymes that have been implicated in a wide range of cellular functions...
  7. ncbi Lipid-induced insulin resistance in human muscle is associated with changes in diacylglycerol, protein kinase C, and IkappaB-alpha
    Samar I Itani
    Diabetes Unit, Section of Endocrinology, Department of Medicine, Boston University Medical Center, Boston, MA, USA
    Diabetes 51:2005-11. 2002
    ..that lipid-induced insulin resistance in human muscle is related to alterations in diacylglycerol (DAG)/protein kinase C (PKC) signaling was investigated in normal volunteers during euglycemic-hyperinsulinemic clamping in which ..
  8. ncbi Mechanism of hepatic insulin resistance in non-alcoholic fatty liver disease
    Varman T Samuel
    Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    J Biol Chem 279:32345-53. 2004
    ....
  9. pmc Prkcz null mice show normal learning and memory
    Anna M Lee
    Ernest Gallo Clinic and Research Center, Department of Neurology, University of California, San Francisco, 5858 Horton Street, Suite 200, Emeryville, California 94608, USA
    Nature 493:416-9. 2013
    Protein kinase M-ζ (PKM-ζ) is a constitutively active form of atypical protein kinase C that is exclusively expressed in the brain and implicated in the maintenance of long-term memory...
  10. ncbi High glucose level and free fatty acid stimulate reactive oxygen species production through protein kinase C--dependent activation of NAD(P)H oxidase in cultured vascular cells
    T Inoguchi
    Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushyu University, Fukuoka, Japan
    Diabetes 49:1939-45. 2000
    ..Treatment of the cells for 3 h with phorbol myristic acid (PMA), a protein kinase C (PKC) activator, also increased free radical production...
  11. pmc Regulation of the phagocyte NADPH oxidase activity: phosphorylation of gp91phox/NOX2 by protein kinase C enhances its diaphorase activity and binding to Rac2, p67phox, and p47phox
    Houssam Raad
    INSERM U773, Centre de Recherche Biomedicale Bichat Beaujon CRB3, Universite Paris 7, Paris, France
    FASEB J 23:1011-22. 2009
    ..The protein kinase C inhibitor GF109203X inhibited phorbol 12-myristate 13-acetate-induced phosphorylation of gp91(phox), and ..
  12. ncbi Storage of spatial information by the maintenance mechanism of LTP
    Eva Pastalkova
    Departments of Physiology, Pharmacology, and Neurology, Robert F Furchgott Center for Neural and Behavioral Science, SUNY Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, NY 11203, USA
    Science 313:1141-4. 2006
    ..This was not previously possible. Recently however, persistent phosphorylation by the atypical protein kinase C isoform, protein kinase Mzeta (PKMz), has been found to maintain late LTP in hippocampal slices...
  13. pmc Par6B and atypical PKC regulate mitotic spindle orientation during epithelial morphogenesis
    Joanne Durgan
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
    J Biol Chem 286:12461-74. 2011
    ..Here, we report that Par6B and its binding partner, atypical protein kinase C (aPKC), are required to regulate Caco-2 morphogenesis...
  14. ncbi The PAR-aPKC system: lessons in polarity
    Atsushi Suzuki
    Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, 3 9 Fuku ura, Kanazawa Ku, Yokohama 236 0004, Japan
    J Cell Sci 119:979-87. 2006
    ....
  15. ncbi Crosstalk of tight junction components with signaling pathways
    Lorenza Gonzalez-Mariscal
    Center for Research and Advanced Studies CINVESTAV, Department of Physiology, Biophysics and Neuroscience, Ave Instituto Politecnico Nacional 2508, Mexico D F 07360, Mexico
    Biochim Biophys Acta 1778:729-56. 2008
    ..In this review we analyze how the crosstalk of protein kinase C, protein kinase A, myosin light chain kinase, mitogen-activated protein kinases, phosphoinositide 3-kinase ..
  16. pmc E-cadherin is essential for in vivo epidermal barrier function by regulating tight junctions
    Judith A Tunggal
    Center for Molecular Medicine, University of Cologne CMMC, Cologne, Germany
    EMBO J 24:1146-56. 2005
    ..Surprisingly, our results indicate that E-cadherin is specifically required for tight junction, but not desmosome, formation and this appears to involve signalling rather than cell contact formation...
  17. pmc Protein kinase C theta affects Ca2+ mobilization and NFAT cell activation in primary mouse T cells
    Christa Pfeifhofer
    Institute of Medical Biology and Human Genetics, University of Innsbruck, Schoepfstrasse 41, A 6020 Innsbruck, Austria
    J Exp Med 197:1525-35. 2003
    b>Protein kinase C (PKC)theta is an established component of the immunological synapse and has been implicated in the control of AP-1 and NF-kappaB...
  18. pmc Inhibition of PKMzeta in nucleus accumbens core abolishes long-term drug reward memory
    Yan qin Li
    National Institute on Drug Dependence, Peking University, Beijing 100191, China
    J Neurosci 31:5436-46. 2011
    ..The mechanisms underlying the maintenance of these memories are unknown. A constitutively active atypical protein kinase C (PKC) isozyme, protein kinase M ζ (PKMζ), is required for maintenance of spatial memory, conditioned taste ..
  19. pmc A cascade of protein kinase C isozymes promotes cytoskeletal polarization in T cells
    Emily J Quann
    Immunology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA
    Nat Immunol 12:647-54. 2011
    ..By single-cell photoactivation of the T cell antigen receptor (TCR), we found that three distinct isoforms of protein kinase C (PKC) were recruited by DAG to the IS in two steps...
  20. pmc Cellular pharmacology of protein kinase Mζ (PKMζ) contrasts with its in vitro profile: implications for PKMζ as a mediator of memory
    Alyssa X Wu-Zhang
    Department of Pharmacology, University of California San Diego, La Jolla, California 92093, USA
    J Biol Chem 287:12879-85. 2012
    ....
  21. ncbi The cell-polarity protein Par6 links Par3 and atypical protein kinase C to Cdc42
    G Joberty
    Markey Center for Cell Signaling, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908 0577, USA
    Nat Cell Biol 2:531-9. 2000
    ..Cdc42-GTP, with a human homologue of the multi-PDZ protein PAR-3 and with the regulatory domains of atypical protein kinase C (PKC) proteins...
  22. pmc The mammalian target of rapamycin complex 2 controls folding and stability of Akt and protein kinase C
    Valeria Facchinetti
    Department of Immunology, The University of Texas, MD Anderson Cancer Center, Houston, TX 77030, USA
    EMBO J 27:1932-43. 2008
    ..SIN1 and rictor, components of mammalian (m)TORC2, are required for phosphorylation of Akt and conventional protein kinase C (PKC) at the turn motif (TM) site...
  23. pmc Crumbs regulates Salvador/Warts/Hippo signaling in Drosophila via the FERM-domain protein Expanded
    Brian S Robinson
    Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA
    Curr Biol 20:582-90. 2010
    ....
  24. ncbi Protein kinase C isotypes controlled by phosphoinositide 3-kinase through the protein kinase PDK1
    J A Le Good
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Science 281:2042-5. 1998
    ..The activation loop phosphorylation of PKCdelta in response to serum stimulation of cells was PI 3-kinase-dependent and was enhanced by PDK1 coexpression...
  25. pmc PKM zeta maintains late long-term potentiation by N-ethylmaleimide-sensitive factor/GluR2-dependent trafficking of postsynaptic AMPA receptors
    Yudong Yao
    The Robert F Furchgott Center for Neural and Behavioral Science, Department of Physiology, State University of New York, Downstate Medical Center, Brooklyn, New York 11203, USA
    J Neurosci 28:7820-7. 2008
    ..The autonomously active protein kinase C isoform, protein kinase Mzeta (PKMzeta), is a core molecule maintaining late-LTP...
  26. ncbi Rapid erasure of long-term memory associations in the cortex by an inhibitor of PKM zeta
    Reut Shema
    Department of Neurobiology, Weizmann Institute of Science, Rehovot 76100, Israel
    Science 317:951-3. 2007
    ..rat cortex, long-term associative memories vanished rapidly after local application of an inhibitor of the protein kinase C isoform, protein kinase M zeta (PKMzeta)...
  27. doi Tumor type-dependent function of the par3 polarity protein in skin tumorigenesis
    Sandra Iden
    Division of Cell Biology I, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    Cancer Cell 22:389-403. 2012
    ..Our results identify a dual function of Par3 in skin cancer, with both pro-oncogenic and tumor-suppressive activity depending on the tumor type...
  28. doi PKMzeta maintains memories by regulating GluR2-dependent AMPA receptor trafficking
    Paola Virginia Migues
    Department of Psychology, McGill University, Montreal, Quebec, Canada
    Nat Neurosci 13:630-4. 2010
    ..of long-term memory in hippocampus, neocortex and amygdala requires the persistent action of the atypical protein kinase C isoform, protein kinase Mzeta (PKMzeta)...
  29. pmc Exacerbated vein graft arteriosclerosis in protein kinase Cdelta-null mice
    M Leitges
    Max Planck Institute for Experimental Endocrinology, Hannover, Germany
    J Clin Invest 108:1505-12. 2001
    ..Because members of the protein kinase C (PKC) family signal cells to undergo proliferation, differentiation, or apoptosis, we generated PKCdelta ..
  30. pmc Protein kinase C, an elusive therapeutic target?
    Daria Mochly-Rosen
    Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, California 94305 5174, USA
    Nat Rev Drug Discov 11:937-57. 2012
    b>Protein kinase C (PKC) has been a tantalizing target for drug discovery ever since it was first identified as the receptor for the tumour promoter phorbol ester in 1982...
  31. doi Oxidized low-density lipoprotein activates p66Shc via lectin-like oxidized low-density lipoprotein receptor-1, protein kinase C-beta, and c-Jun N-terminal kinase kinase in human endothelial cells
    Yi Shi
    Cardiovascular Research, Institute of Physiology, University of Zurich, Zurich, Switzerland
    Arterioscler Thromb Vasc Biol 31:2090-7. 2011
    ..The present study was designed to elucidate the proatherogenic mechanisms by which p66(Shc) mediates oxidized low-density lipoprotein (oxLDL) uptake by the endothelium, a critical step in plaque formation...
  32. pmc TrkB and protein kinase Mζ regulate synaptic localization of PSD-95 in developing cortex
    Akira Yoshii
    Departmens of Brain and Cognitive Science, McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Neurosci 31:11894-904. 2011
    ..These results also indicate that palmitoylation can be regulated by a trophic factor. Our findings have implications for neurodevelopmental disorders as well as aging brains...
  33. pmc Activation of dual oxidases Duox1 and Duox2: differential regulation mediated by camp-dependent protein kinase and protein kinase C-dependent phosphorylation
    Sabrina Rigutto
    Institut de Recherche Interdisciplinaire en Biologie Humaine et Moleculaire, Université Libre Bruxelles, Brussels, Belgium
    J Biol Chem 284:6725-34. 2009
    ..In contrast, phorbol esters induce Duox2 phosphorylation via protein kinase C activation associated with high H(2)O(2) generation (phorbol 12-myristate 13-acetate EC(50) = 0.8 nm)...
  34. pmc Protein kinase C: poised to signal
    Alexandra C Newton
    Dept of Pharmacology, Univ of California at San Diego, La Jolla, 92093, USA
    Am J Physiol Endocrinol Metab 298:E395-402. 2010
    Nestled at the tip of a branch of the kinome, protein kinase C (PKC) family members are poised to transduce signals emanating from the cell surface...
  35. ncbi Par-3 controls tight junction assembly through the Rac exchange factor Tiam1
    Xinyu Chen
    Center for Cell Signaling, Department of Microbiology, HSC, University of Virginia, Charlottesville, Virginia 22908, USA
    Nat Cell Biol 7:262-9. 2005
    ..Par-3 has multiple protein-interaction domains, and associates with Par-6 and atypical protein kinase C (aPKC). In Drosophila, Par-3 is essential for epithelial cell polarization...
  36. ncbi Intermittent high glucose enhances apoptosis related to oxidative stress in human umbilical vein endothelial cells: the role of protein kinase C and NAD(P)H-oxidase activation
    Lisa Quagliaro
    Morpurgo Hofman Research Laboratory on Aging, Udine, Italy
    Diabetes 52:2795-804. 2003
    ..However, these effects were more pronounced in intermittent high glucose. Protein kinase C (PKC) was elevated in both such conditions, particularly in intermittent glucose...
  37. pmc Protein kinases C and D mediate agonist-dependent cardiac hypertrophy through nuclear export of histone deacetylase 5
    Rick B Vega
    Department of Molecular Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9148, USA
    Mol Cell Biol 24:8374-85. 2004
    ..Here, we demonstrate that signaling by protein kinase C (PKC) is sufficient and, in some cases, necessary to drive nuclear export of class II HDAC5 in cardiomyocytes...
  38. pmc The fission yeast pmk1+ gene encodes a novel mitogen-activated protein kinase homolog which regulates cell integrity and functions coordinately with the protein kinase C pathway
    T Toda
    Cell Regulation Laboratory, Imperial Cancer Research Fund, London, United Kingdom
    Mol Cell Biol 16:6752-64. 1996
    ..In Saccharomyces cerevisiae, Mpk1 is involved in cell wall integrity and functions downstream of the protein kinase C homolog. In contrast, in S...
  39. pmc Extracellular signal-regulated kinase phosphorylates tumor necrosis factor alpha-converting enzyme at threonine 735: a potential role in regulated shedding
    Elena Diaz-Rodriguez
    Instituto de Microbiología Bioquímica and Centro de Investigación del Cáncer, Consejo Superior de Investigaciones Cientificas Universidad de Salamanca, 37007 Salamanca, Spain
    Mol Biol Cell 13:2031-44. 2002
    ..We show that extracellular signal-regulated kinase (Erk) acts as an intermediate in protein kinase C-regulated TrkA cleavage...
  40. pmc Par3 controls epithelial spindle orientation by aPKC-mediated phosphorylation of apical Pins
    Yi Hao
    Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Curr Biol 20:1809-18. 2010
    ..Defects in signaling through Cdc42 and atypical protein kinase C (aPKC) also cause spindle misorientation...
  41. doi How does PKMζ maintain long-term memory?
    Todd C Sacktor
    The Robert F Furchgott Center of Neural and Behavioural Science, Department of Physiology, State University of New York Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, New York 11203, USA
    Nat Rev Neurosci 12:9-15. 2011
    ..Recent research shows how PKMζ might perpetuate information both at synapses and during long-term memory...
  42. ncbi PKC-1 regulates secretion of neuropeptides
    Derek Sieburth
    Department of Molecular Biology, Simches 7, Massachusetts General Hospital, 185 Cambridge St, Boston, Massachusetts 02114, USA
    Nat Neurosci 10:49-57. 2007
    ..Here we show that protein kinase C-1 (PKC-1), which is most similar to the vertebrate PKC eta and epsilon isoforms, regulates exocytosis of DCVs ..
  43. ncbi Immunodeficiency in protein kinase cbeta-deficient mice
    M Leitges
    Max Delbruck Laboratorium in der Max Planck Gesellschaft, Carl von Linne Weg 10, D 50829 Köln, Germany
    Science 273:788-91. 1996
    ..receptor on lymphocytes by antigens or antibodies to the receptor results in activation of enzymes of the protein kinase C (PKC) family...
  44. pmc A motif in the V3 domain of the kinase PKC-θ determines its localization in the immunological synapse and functions in T cells via association with CD28
    Kok Fai Kong
    Division of Cell Biology, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA
    Nat Immunol 12:1105-12. 2011
    b>Protein kinase C-θ (PKC-θ) translocates to the center of the immunological synapse, but the underlying mechanism and its importance in T cell activation are unknown...
  45. pmc Phosphorylation of histone H3 at threonine 11 establishes a novel chromatin mark for transcriptional regulation
    Eric Metzger
    Universitäts Frauenklinik und Zentrale Klinische Forschung, Klinikum der Universität Freiburg, Breisacherstrasse 66, 79106 Freiburg, Germany
    Nat Cell Biol 10:53-60. 2008
    ..Finally, inhibition of PRK1 blocks proliferation of androgen receptor-induced tumour cell proliferation, making PRK1 a promising therapeutic target...
  46. pmc Atypical protein kinase C (aPKCzeta and aPKClambda) is dispensable for mammalian hematopoietic stem cell activity and blood formation
    Amitava Sengupta
    Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    Proc Natl Acad Sci U S A 108:9957-62. 2011
    ..orchestrated by a polarity complex consisting of partitioning-defective proteins Par3 and Par6, and atypical protein kinase C (aPKCζ and aPKCλ), which regulates planar symmetry of dividing stem cells with respect to the signaling ..
  47. ncbi Protein kinase C beta and prolyl isomerase 1 regulate mitochondrial effects of the life-span determinant p66Shc
    Paolo Pinton
    Department of Experimental and Diagnostic Medicine, Section of General Pathology and Interdisciplinary Center for the Study of Inflammation ICSI, University of Ferrara, Ferrera, Italy
    Science 315:659-63. 2007
    ..We demonstrate that protein kinase C beta, activated by oxidative conditions in the cell, induces phosphorylation of p66Shc and triggers ..
  48. ncbi Protein kinase C and the regulation of the actin cytoskeleton
    Christer Larsson
    Lund University, Dept of Laboratory Medicine, Molecular Medicine, Entrance 78, 3rd Floor, UMAS SE 205 02, Malmo University Hospital, Malmo, Sweden
    Cell Signal 18:276-84. 2006
    b>Protein kinase C (PKC) isoforms are central components in intracellular networks that regulate a vast number of cellular processes...
  49. doi Positive feedback and mutual antagonism combine to polarize Crumbs in the Drosophila follicle cell epithelium
    Georgina C Fletcher
    Cancer Research UK, London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3LY, UK
    Curr Biol 22:1116-22. 2012
    ..Once Crb is removed from the plasma membrane, it undergoes recycling via Rab11 endosomes. Our results provide a dynamic model for understanding how epithelial polarity is maintained in Drosophila follicle cells...
  50. pmc Protein kinase cβ phosphorylates occludin regulating tight junction trafficking in vascular endothelial growth factor-induced permeability in vivo
    Tomoaki Murakami
    Department of Cellular and Molecular Physiology and Ophthalmology, Penn State College of Medicine, Hershey, Pennsylvania, USA
    Diabetes 61:1573-83. 2012
    Vascular endothelial growth factor (VEGF)-induced breakdown of the blood-retinal barrier requires protein kinase C (PKC)β activation. However, the molecular mechanisms related to this process remain poorly understood...
  51. doi Osthole regulates inflammatory mediator expression through modulating NF-κB, mitogen-activated protein kinases, protein kinase C, and reactive oxygen species
    Pei Chun Liao
    Institute of Biotechnology, National Ilan University, Ilan, Taiwan
    J Agric Food Chem 58:10445-51. 2010
    ..Osthole also inhibited NF-κB activation and ROS release in LPS-stimulated macrophages. Our current results indicated that osthole is the major anti-inflammatory ingredient of Cnidium monnieri seed ethanol extract...
  52. pmc Emerging roles of protein kinase D1 in cancer
    Vasudha Sundram
    Cancer Biology Research Center, Sanford Research USD, University of South Dakota, Sioux Falls, South Dakota 57105, USA
    Mol Cancer Res 9:985-96. 2011
    ..In this review, we summarize the structural and functional aspects of PKD1 and highlight the pathobiological roles of this kinase in cancer...
  53. pmc Protein kinase D as a potential new target for cancer therapy
    Courtney R LaValle
    Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Biochim Biophys Acta 1806:183-92. 2010
    ..This review focuses on the potential of PKD as a chemotherapeutic target in cancer treatment and highlights important recent advances in the development of PKD inhibitors...
  54. pmc Protein kinase C-theta regulates KIT expression and proliferation in gastrointestinal stromal tumors
    W B Ou
    1Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Oncogene 27:5624-34. 2008
    ..We reported previously that protein kinase C-theta (PKCtheta) is activated in GIST, irrespective of KIT or PDGFRA mutational status, and is expressed at ..
  55. doi Anti-inflammatory bioactivities of honokiol through inhibition of protein kinase C, mitogen-activated protein kinase, and the NF-kappaB pathway to reduce LPS-induced TNFalpha and NO expression
    Louis Kuoping Chao
    Department of Cosmeceutics, China Medical University, Taichung, Taiwan
    J Agric Food Chem 58:3472-8. 2010
    ..By means of confocal microscope analysis we demonstrated that phosphorylation and membrane translocation of protein kinase C-alpha, as well as NF-kappaB activation, were inhibited by honokiol in LPS-stimulated macrophages...
  56. pmc Altered phosphorylation of cytoskeleton proteins in sickle red blood cells: the role of protein kinase C, Rac GTPases, and reactive oxygen species
    Alex George
    Cancer and Blood Diseases Institute, Cincinnati Children s Hospital Medical Center and University of Cincinnati College of Medicine, Cincinnati, OH, USA
    Blood Cells Mol Dis 45:41-5. 2010
    ..phosphorylation of adducin at Ser-724, (corresponding to Ser-726 in human erythrocytes), a domain target of protein kinase C (PKC)...
  57. ncbi Persistent phosphorylation by protein kinase Mzeta maintains late-phase long-term potentiation
    Peter Serrano
    Department of Physiology, State University of New York Downstate Medical Center, Brooklyn, New York 11203, USA
    J Neurosci 25:1979-84. 2005
    ..An inactive scrambled version of the peptide had no effect on LTP. Thus, persistent, increased phosphorylation by PKMzeta specifically maintains the late phase of LTP...
  58. doi PKC inhibitors: potential in T cell-dependent immune diseases
    Gottfried Baier
    Department for Medical Genetics, Molecular and Clinical Pharmacology, Innsbruck Medical University, Innsbruck, Austria
    Curr Opin Cell Biol 21:262-7. 2009
    ..Members of the protein kinase C (PKC) family are crucial in T cell signaling pathways...
  59. doi Chromatin-associated protein kinase C-θ regulates an inducible gene expression program and microRNAs in human T lymphocytes
    Elissa L Sutcliffe
    Department of Immunology, John Curtin School of Medical Research, Australian National University, Canberra, ACT 2601, Australia
    Mol Cell 41:704-19. 2011
    ..Here, we provide evidence for a hitherto unknown function of protein kinase C-theta (PKC-θ), which physically associates with the regulatory regions of inducible immune response genes in ..
  60. pmc PKMζ is essential for spinal plasticity underlying the maintenance of persistent pain
    Andre Laferriere
    Department of Anesthesia, McGill University, 3655 Promenade Sir William Osler, Montreal, Quebec, H3G 1Y6, Canada
    Mol Pain 7:99. 2011
    ..We examined here whether an isoform of protein kinase C (PKC), PKMζ, that underlies long-term memory storage in various brain regions, also sustains nociceptive ..
  61. pmc Spinal protein kinase M ζ underlies the maintenance mechanism of persistent nociceptive sensitization
    Marina N Asiedu
    Department of Pharmacology, The University of Arizona College of Medicine, Tucson, AZ 85721, USA
    J Neurosci 31:6646-53. 2011
    ....
  62. ncbi Regulation of the polarity protein Par6 by TGFbeta receptors controls epithelial cell plasticity
    Barish Ozdamar
    Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Canada
    Science 307:1603-9. 2005
    ..These studies define how an extracellular cue signals to the polarity machinery to control epithelial cell morphology...
  63. pmc Syk kinase-coupled C-type lectin receptors engage protein kinase C-σ to elicit Card9 adaptor-mediated innate immunity
    Dominikus Strasser
    Institut fur Klinische Chemie und Pathobiochemie, Klinikum rechts der Isar, Technische Universitat Munchen, Munich, Germany
    Immunity 36:32-42. 2012
    ..The mechanisms relaying CLR proximal signals to the core Card9 module are unknown. Here we demonstrated that protein kinase C-δ (PKCδ) was activated upon Dectin-1-Syk signaling, mediated phosphorylation of Card9 at Thr231, and was ..
  64. pmc Cell wall integrity signaling in Saccharomyces cerevisiae
    David E Levin
    Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    Microbiol Mol Biol Rev 69:262-91. 2005
    ....
  65. pmc Asymmetric cell divisions promote stratification and differentiation of mammalian skin
    Terry Lechler
    Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA
    Nature 437:275-80. 2005
    ..We further demonstrate that integrins and cadherins are essential for the apical localization of atypical protein kinase C, the Par3-LGN-Inscuteable complex and NuMA-dynactin to align the spindle.
  66. ncbi Role of aPKC isoforms and their binding partners Par3 and Par6 in epidermal barrier formation
    Iris Helfrich
    Center for Molecular Medicine Cologne, University of Cologne, Cologne, Germany
    J Invest Dermatol 127:782-91. 2007
    ..Indeed, inhibition of aPKC function showed that endogenous aPKC is crucial for in vitro barrier function and this required the presence of both the Par3 and Par6 binding sites...
  67. pmc Ezrin is a downstream effector of trafficking PKC-integrin complexes involved in the control of cell motility
    T Ng
    Richard Dimbleby Department of Cancer Research, St Thomas Hospital, Lambeth Palace Road, London SE1 7EH, UK
    EMBO J 20:2723-41. 2001
    b>Protein kinase C (PKC) alpha has been implicated in beta1 integrin-mediated cell migration. Stable expression of PKCalpha is shown here to enhance wound closure...
  68. ncbi Protein kinase C modulates pulmonary endothelial permeability: a paradigm for acute lung injury
    Alma Siflinger-Birnboim
    Research Service, Stratton Veterans Affairs Medical Center and the Center for Cardiovascular Science, The Albany Medical College, Albany, New York 12208, USA
    Am J Physiol Lung Cell Mol Physiol 284:L435-51. 2003
    The intracellular serine/threonine kinase protein kinase C (PKC) has an important role in the genesis of pulmonary edema...
  69. ncbi Phosphorylation of p47phox sites by PKC alpha, beta II, delta, and zeta: effect on binding to p22phox and on NADPH oxidase activation
    Alexandre Fontayne
    Institut National de la Santé et de la Recherche Médicale U 479, Centre Hospitalier Universitaire Xavier Bichat, Paris, France
    Biochemistry 41:7743-50. 2002
    ..p47(phox) is an excellent substrate for protein kinase C (PKC), but the respective contribution of each PKC isoform to this process is not clearly defined...
  70. ncbi Pathogenesis of diabetic nephropathy: the role of oxidative stress and protein kinase C
    H Ha
    Department of Pharmacology, Yonsei University College of Medicine, Seoul, South Korea
    Diabetes Res Clin Pract 45:147-51. 1999
    ..Since the inhibition of protein kinase C (PKC) effectively blocks not only phorbol ester-induced but also high glucose- and H2O2-induced fibronectin ..
  71. ncbi Regulation of highly cytokinergic IgE-induced mast cell adhesion by Src, Syk, Tec, and protein kinase C family kinases
    Jiro Kitaura
    Division of Cell Biology, La Jolla Institute for Allergy and Immunology, San Diego, CA 92121, USA
    J Immunol 174:4495-504. 2005
    ..Importantly, we have identified Lyn and Syk as requisite tyrosine kinases and Hck, Btk, and protein kinase C theta as contributory kinases in HC IgE-induced adhesion and spreading, whereas protein kinase C epsilon ..
  72. ncbi Protein kinase Ctheta: a new essential superstar on the T-cell stage
    A Altman
    Division of Cell Biology, La Jolla Institute for Allergy and Immunology, San Diego, CA 92121, USA
    Immunol Today 21:567-73. 2000
    Recent studies have identified protein kinase Cθ (PKCtheta), a member of the Ca(2+)-independent PKC family, as an essential component of the T-cell synapse that cooperates with calcineurin to activate the interleukin-2 (IL-2) ..
  73. pmc PKC eta regulates occludin phosphorylation and epithelial tight junction integrity
    Takuya Suzuki
    Department of Physiology, University of Tennessee Health Science Center, 894 Union Avenue, Memphis, TN 38163, USA
    Proc Natl Acad Sci U S A 106:61-6. 2009
    ..These results reveal an important mechanism of epithelial TJ regulation by PKC eta...
  74. doi Role of secondary sensory cortices in emotional memory storage and retrieval in rats
    Tiziana Sacco
    Department of Neuroscience, University of Turin, Corso Raffaello 30, I 10125 Turin, Italy
    Science 329:649-56. 2010
    ..We propose that secondary sensory cortices support memory storage and retrieval of sensory stimuli that have acquired a behavioral salience with the experience...
  75. ncbi Protein kinase D1: a protein of emerging translational interest
    Meena Jaggi
    Sanford Research USD, Cancer Biology Research Institute, Department of OBGYN and Basic Biomedical Science, Sanford School of Medicine of The University of South Dakota and Sioux Valley Hospitals and Health Systems, Sioux Falls, SD 57103, USA
    Front Biosci 12:3757-67. 2007
    ..In this review, while addressing the molecular aspects of PKD1, we will also highlight the potential translational implication of PKD1 function in human diseases...
  76. ncbi Mechanism of diacylglycerol-induced membrane targeting and activation of protein kinase Cdelta
    Robert V Stahelin
    Department of Chemistry, University of Illinois, Chicago, Illinois 60607, USA
    J Biol Chem 279:29501-12. 2004
    ..Collectively, these results provide new insight into the differential roles of C1 domains in the DAG-induced membrane activation of PKCdelta and the origin of its specific subcellular localization in response to DAG...
  77. ncbi Prostratin antagonizes HIV latency by activating NF-kappaB
    Samuel A Williams
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, San Francisco, California 94141, USA
    J Biol Chem 279:42008-17. 2004
    ..transducers demonstrates that prostratin stimulates membrane translocation of classical, novel, and atypical protein kinase C (PKC) isoforms...
  78. pmc Regulation of the antioxidant response element by protein kinase C-mediated phosphorylation of NF-E2-related factor 2
    H C Huang
    Schering Plough Research Institute, Kenilworth, NJ 07033, USA
    Proc Natl Acad Sci U S A 97:12475-80. 2000
    ..13-acetate (PMA), but completely suppressed by staurosporine and Ro-32-0432, selective inhibitors of protein kinase C (PKC)...
  79. ncbi Intermittent high glucose enhances ICAM-1, VCAM-1 and E-selectin expression in human umbilical vein endothelial cells in culture: the distinct role of protein kinase C and mitochondrial superoxide production
    Lisa Quagliaro
    Morpurgo Hofman Research Laboratory on Aging, Udine, Italy
    Atherosclerosis 183:259-67. 2005
    ....
  80. pmc Corticotropin-releasing factor increases mouse ventral tegmental area dopamine neuron firing through a protein kinase C-dependent enhancement of Ih
    M J Wanat
    Ernest Gallo Clinic and Research Center, 5858 Horton St, Suite 200, Emeryville, CA 94608, USA
    J Physiol 586:2157-70. 2008
    ..Inhibition of the phospholipase C (PLC)-protein kinase C (PKC) signalling pathway, but not the cAMP-protein kinase A (PKA) signalling pathway, prevented the increase ..
  81. ncbi Protein kinase C(theta) in T cell activation
    Noah Isakov
    Department of Microbiology and Immunology, Faculty of Health Sciences, and the Cancer Research Center, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    Annu Rev Immunol 20:761-94. 2002
    The novel protein kinase C (PKC) isoform, PKC theta, is selectively expressed in T lymphocytes and is a sine qua non for T cell antigen receptor (TCR)-triggered activation of mature T cells...
  82. ncbi The role of protein kinase C activation and the vascular complications of diabetes
    Net Das Evcimen
    Ankara University, Pharmacy Faculty, Biochemistry Department, 06100 Ankara, Turkey
    Pharmacol Res 55:498-510. 2007
    ..altered cellular redox state, increased formation of diacylglycerol (DAG) and the subsequent activation of protein kinase C (PKC) isoforms and accelerated non-enzymatic formation of advanced glycated end products...
  83. ncbi Coordinated control of endothelial nitric-oxide synthase phosphorylation by protein kinase C and the cAMP-dependent protein kinase
    B J Michell
    St. Vincent's Institute of Medical Research, St. Vincent's Hospital, 41 Victoria Parade, Fitzroy, Victoria 3065, Australia
    J Biol Chem 276:17625-8. 2001
    ..These results show that regulation of eNOS activity involves coordinated signaling through Ser-1177 and Thr-495 by multiple protein kinases and phosphatases...
  84. pmc CD3/CD28 costimulation-induced NF-kappaB activation is mediated by recruitment of protein kinase C-theta, Bcl10, and IkappaB kinase beta to the immunological synapse through CARMA1
    Donghai Wang
    Department of Microbiology and Immunology, School of Medicine and Biomedical Sciences, University at Buffalo, SUNY, Buffalo, New York 14214, USA
    Mol Cell Biol 24:164-71. 2004
    ..induces the formation of the immunological synapse in CARMA1-deficient T cells, the recruitment of protein kinase C-theta (PKC-theta), Bcl10, and IkappaB kinase beta (IKKbeta) into lipid rafts of the immunological synapse is ..
  85. ncbi Synaptic incorporation of AMPA receptors during LTP is controlled by a PKC phosphorylation site on GluR1
    Jannic Boehm
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Neuron 51:213-25. 2006
    ..We conclude that the phosphorylation of GluR1 S818 by PKC is a critical event in the plasticity-driven synaptic incorporation of AMPA receptors...
  86. ncbi Insulin-sensitive protein kinases (atypical protein kinase C and protein kinase B/Akt): actions and defects in obesity and type II diabetes
    Robert V Farese
    James A Haley Veterans Administration Hospital Research Service and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, FL 33612, USA
    Exp Biol Med (Maywood) 230:593-605. 2005
    ..3-kinase (PI3K) which, via increases in PI-3,4,5-triphosphate (PIP(3)), activates atypical protein kinase C (aPKC) and protein kinase B (PKB/Akt)...
  87. ncbi Cortical flows powered by asymmetrical contraction transport PAR proteins to establish and maintain anterior-posterior polarity in the early C. elegans embryo
    Edwin Munro
    Center for Cell Dynamics, Friday Harbor, WA 98250, USA
    Dev Cell 7:413-24. 2004
    ..Similar actomyosin flows accompany the establishment of PAR asymmetries that form after the one-cell stage, suggesting that actomyosin-mediated cortical flows have a general role in PAR asymmetry...
  88. pmc Proteome-wide overexpression of host proteins for identification of factors affecting tombusvirus RNA replication: an inhibitory role of protein kinase C
    Muhammad Shah Nawaz-Ul-Rehman
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky, USA
    J Virol 86:9384-95. 2012
    ....
  89. pmc PKC alpha regulates the hypertrophic growth of cardiomyocytes through extracellular signal-regulated kinase1/2 (ERK1/2)
    Julian C Braz
    Department of Pediatrics, University of Cincinnati, Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    J Cell Biol 156:905-19. 2002
    Members of the protein kinase C (PKC) isozyme family are important signal transducers in virtually every mammalian cell type...
  90. pmc Protein kinase D1 regulates cofilin-mediated F-actin reorganization and cell motility through slingshot
    Tim Eiseler
    Department of Cancer Biology, Mayo Clinic Comprehensive Cancer Center, Mayo Clinic, 4500 San Pablo Road, Jacksonville, Florida 32224, USA
    Nat Cell Biol 11:545-56. 2009
    ..Consequently, expression of constitutively active PKD1 in invasive tumour cells enhanced the phosphorylation of cofilin and effectively blocked the formation of free actin-filament barbed ends and directed cell migration...
  91. pmc Essential function of TORC2 in PKC and Akt turn motif phosphorylation, maturation and signalling
    Tsuneo Ikenoue
    Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA
    EMBO J 27:1919-31. 2008
    b>Protein kinase C (PKC) is involved in a wide array of cellular processes such as cell proliferation, differentiation and apoptosis. Phosphorylation of both turn motif (TM) and hydrophobic motif (HM) are important for PKC function...
  92. pmc Atypical protein kinase C phosphorylates Par6 and facilitates transforming growth factor β-induced epithelial-to-mesenchymal transition
    Adrian Gunaratne
    Department of Physiology and Pharmacology, Western University, London, Ontario, Canada
    Mol Cell Biol 33:874-86. 2013
    ..We show here that atypical protein kinase C (aPKC) is an essential component to this signaling pathway in non-small-cell lung cancer (NSCLC) cells...
  93. pmc N,N'-dinitrosopiperazine-mediated ezrin protein phosphorylation via activation of Rho kinase and protein kinase C is involved in metastasis of nasopharyngeal carcinoma 6-10B cells
    Faqing Tang
    Medical Research Center, Zhuhai Hospital, Guangzhou 510000, Guangdong, China
    J Biol Chem 286:36956-67. 2011
    ..Furthermore, DNP-induced phos-ezrin expression was dependent on increased Rho kinase and protein kinase C (PKC) activity...
  94. pmc GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas
    Scott X Atwood
    Program in Epithelial Biology, Stanford University School of Medicine, Stanford, California 94305, USA
    Nature 494:484-8. 2013
    ..Here we identify atypical protein kinase C ι/λ (aPKC-ι/λ) as a novel GLI regulator in mammals...
  95. ncbi Determination of the specific substrate sequence motifs of protein kinase C isozymes
    K Nishikawa
    Division of Signal Transduction, Beth Israel Hospital, Boston, Massachusetts 02115, USA
    J Biol Chem 272:952-60. 1997
    b>Protein kinase C (PKC) family members play significant roles in a variety of intracellular signal transduction processes, but information about the substrate specificities of each PKC family member is quite limited...
  96. pmc Nerve growth factor enhances the excitability of rat sensory neurons through activation of the atypical protein kinase C isoform, PKMζ
    Y H Zhang
    Dept of Pharmacology and Toxicology, Indiana Univ School of Medicine, Indianapolis, IN 46202, USA
    J Neurophysiol 107:315-35. 2012
    ..These results demonstrate that NGF leads to the activation of PKMζ that ultimately enhances the capacity of small-diameter capsaicin-sensitive sensory neurons to fire APs through a PI3K-dependent signaling cascade...
  97. ncbi Molecular cloning and characterization of PKC iota, an atypical isoform of protein kinase C derived from insulin-secreting cells
    L A Selbie
    Garvan Institute of Medical Research, St Vincent s Hospital, Sydney, Australia
    J Biol Chem 268:24296-302. 1993
    The protein kinase C (PKC) family of serine-threonine kinases comprises at least eight members...
  98. pmc Group I metabotropic glutamate receptors control metaplasticity of spinal cord learning through a protein kinase C-dependent mechanism
    Adam R Ferguson
    Brain and Spinal Injury Center, Department of Neurological Surgery, University of California, San Francisco, San Francisco, California 94110, USA
    J Neurosci 28:11939-49. 2008
    ..has been linked to group I metabotropic glutamate receptors (subtypes mGluR1 and mGluR5) and activation of protein kinase C (PKC)...
  99. pmc Group I mGluR activation reverses cocaine-induced accumulation of calcium-permeable AMPA receptors in nucleus accumbens synapses via a protein kinase C-dependent mechanism
    James E McCutcheon
    Department of Cellular and Molecular Pharmacology, Rosalind Franklin University of Medicine and Science, The Chicago Medical School, North Chicago, Illinois 60064, USA
    J Neurosci 31:14536-41. 2011
    ..Furthermore, they suggest that activation of mGluR1 may represent a potential strategy for reducing cue-induced cocaine craving in abstinent cocaine addicts...
  100. pmc PKC-θ is a drug target for prevention of T cell-mediated autoimmunity and allograft rejection
    Myung Ja Kwon
    Beckman Research Institute of the City of Hope, Duarte, CA 91010, Los Angeles, USA
    Endocr Metab Immune Disord Drug Targets 10:367-72. 2010
    b>Protein kinase C theta (PKC-θ) is a key kinase in mediating T cell receptor (TCR) signals...
  101. pmc Matching biochemical and functional efficacies confirm ZIP as a potent competitive inhibitor of PKMζ in neurons
    Yudong Yao
    Department of Physiology and Pharmacology, State University of New York Downstate Medical Center, 450 Clarkson Ave, Brooklyn, NY 11203, USA
    Neuropharmacology 64:37-44. 2013
    PKMζ is an autonomously active, atypical protein kinase C (aPKC) isoform that is both necessary and sufficient for maintaining long-term potentiation (LTP) and long-term memory...

Research Grants69

  1. Cellular &Inflammatory Pathways in Ashtma &Allergic Diseases: From IgE to Cells
    LAWRENCE BARRY SCHWARTZ; Fiscal Year: 2012
    ..We will study how Th2 cells are regulated by protein kinase C? (PKC?) in Project 2, how different IgEs differentially affect mast cell activation in Project 3, and how ..
  2. Patient-Derived Stem Cells for Phosphoproteomic Profiling Neuropsychopathology
    Dieter A Wolf; Fiscal Year: 2011
    ..Studies in rat brains have also suggested that lithium administration reduces translocation of Protein Kinase C from the cytosol to the cell membrane {Hahn, 1999 #158}, &inhibits GSK3 activity in mice (Catapano &..
  3. TARGETING THE MARCKS-FAMILY PROTEINS TO INHIBIT NEUTROPHIL MIGRATION
    Mary K Sheats; Fiscal Year: 2013
    ..MARCKS and MARCKS-like 1 (MARCKSL1), are known cell membrane and actin binding proteins regulated by protein kinase C (PKC) phosphorylation and calcium/calmodulin binding...
  4. Alzheimer's Disease Therapeutic
    Trevor P Castor; Fiscal Year: 2012
    ..at The Blanchette Rockefeller Neurosciences Institute, Rockville, MD have hypothesized and shown that protein kinase C (PKC) and its activation are important means of ameliorating AD pathophysiology and cognitive impairment...
  5. Regulation of K-Ras by a Farnesyl-electrostatic Switch
    MARK REID PHILIPS; Fiscal Year: 2013
    ..g. farnesylation) and membrane targeting. We discovered a post-translational modification unique to K-Ras: protein kinase C (PKC) mediated phosphorylation...
  6. Atypical PKC Knockout Models: Effect on Glucose and Lipid Homeostasis
    Robert V Farese; Fiscal Year: 2013
    ..stimulates glucose transport in muscle and adipocytes, and lipid synthesis in liver by activating atypical protein kinase C (aPKC) and Akt (PKB)...
  7. The PKCepsilon-regulated oncogenic and tumor suppressor roles of ATF2 in melanoma
    ERIC KIRK LAU; Fiscal Year: 2013
    ..How ATF2 plays both functions has remained unclear. I recently found that Protein Kinase C, isoform epsilon (PKCepsilon) phosphorylates ATF2 on a novel phosphoacceptor site (T52), promoting its ..
  8. Novel CTD inhibitors with synthetic lethality to oncogenic Ras for cancer therapy
    Bingliang Fang; Fiscal Year: 2013
    ..cytotoxic effects correlated with apoptosis induction by the compounds and could be blocked by K-Ras siRNA or protein kinase C iota (PKCiota) siRNA, suggesting that Ras and/or PKCiota activities are required for oncrasin-induced ..
  9. KCNQ Channels and Vasoconstrictor Signal Transduction
    Kenneth L Byron; Fiscal Year: 2013
    ..The roles of specific protein kinase C isoforms and A kinase-anchoring protein 150 (AKAP150) in KCNQ current regulation will be investigated using ..
  10. Innate Immunity and Cardiovascular Function
    Gregory L Stahl; Fiscal Year: 2013
    ..The protein kinase C, polyol, and advanced glycation end products (AGE) pathways are thought to be responsible for hyperglycemia-..
  11. PKC delta and cell renewal in irradiated taste epithelium
    MARY ELAINE REYLAND; Fiscal Year: 2012
    ..In Aim 2 we will investigate the role of the novel protein kinase C delta isoform (PKC4) in irradiation-induced taste epithelial injury...
  12. StAR expression: Integration of Transcription with regulation via the mRNA 3'UTR
    COLIN ROBERT JEFCOATE; Fiscal Year: 2012
    ..This research focuses on the finding that StAR expression is stimulated in very different ways by PKA and by protein kinase C (PKC)...
  13. Zot, Zonulin, and Pathophysiology of Intestinal Tight Junctions
    Alessio Fasano; Fiscal Year: 2013
    ..We have demonstrated that this transactivation is followed by protein kinase C (PKC)a-mediated ZO-1 and myosin 1c serine/threonine phosphorylation, provoking ZO-1 disengagement from three ..
  14. Innate Immunity and Cardiovascular Function
    Gregory L Stahl; Fiscal Year: 2010
    ..The protein kinase C, polyol and advanced glycation end products (AGE) pathways are thought to be responsible for hyperglycemia-..
  15. REGULATION OF PHOSPHOLIPID SYNTHESIS
    George M Carman; Fiscal Year: 2013
    ..In specific ai 1, we will examine the regulation of Pah1p PAP via phosphorylations by kinase A, protein kinase C, and casein kinase II...
  16. MECHANISMS OF RETINAL VASCULAR PERMEABILITY IN DIABETES
    DAVID ANTONETTI; Fiscal Year: 2013
    ..funding period has demonstrated that VEGF induces phosphorylation of the tight junction protein occludin in a protein kinase C dependent manner that is associated with vascular permeability...
  17. Mechanisms for cell specific modulation of proliferation and migration
    Thomas S Monahan; Fiscal Year: 2013
    ..kinase substrate (MARCKS), a membrane bound protein that translocates to the cytosol upon phosphorylation by protein kinase C. Previous work by the candidate has demonstrated that MARCKS knockdown results in decreased vascular smooth ..
  18. Characterization of the thioredoxins system in the diabetic retina
    Folami Lamoke; Fiscal Year: 2013
    ..Sequence analysis shows that this molecular event is dependent on Protein Kinase C delta (PKC?), a ROS sensitive PKC isoform up-regulated in the diabetic retina and in retinal endothelial ..
  19. Prostate cancer prevention by selenium supplementation
    Rayudu Gopalakrishna; Fiscal Year: 2012
    ..actions, at least in part, through oxidation of unique cysteine- rich regions and zinc fingers present in protein kinase C (PKC), a known target for a variety of tumor promoters...
  20. Human Platelet Defects in Transcription Factor RUNX1 Haplodeficiency
    ANGARA KONETI RAO; Fiscal Year: 2013
    ..deficiencies in three major signaling proteins, phospholipase C-2, the GTP-binding protein Gq and protein kinase C-, and into the platelet dysfunction associated with haplodeficiency of a major transcription factor, RUNX1...
  21. Mechanism and functional role of AKAP79/150 in M current control and excitability
    Mark S Shapiro; Fiscal Year: 2010
    ..One such AKAP, AKAP79/150 interacts with protein kinase A, protein kinase C (PKC), calmodulin (CaM), calcineurin, and phosphatidylinositol 4,5- bisphosphate (PIP2), along with effectors ..
  22. Targeting Tight Junctions To Treat Mucositis
    FREDERICK GARY TOBACK; Fiscal Year: 2010
    ..In cell culture, AMP-18 activates Akt and protein kinase C-zeta (PKC6) which could mediate the observed translocation of cytosolic proteins such as Par6 and Cdc42 into ..
  23. Selective Insulin Resistance- Diabetic Cardiovascular Diseases
    George L King; Fiscal Year: 2013
    ..We have also reported that hyperglycemia and free fatty acids (FFA) can activate protein kinase C (PKC), specifically the 21/2 and 4 isoforms, and induce "selective insulin resistance" in the microvessels or ..
  24. Abnormal Bone Marrow Cells and Diabetic Neuropathy
    Lawrence Chan; Fiscal Year: 2013
    ..and ischemia, activation of the polyol pathway, increase advanced glycation end products, activation of protein kinase C and NFkB and mitogen-activated kinases, and growth factor deficiency...
  25. Protein Kinase C in the Repair of Cellular Functions
    Grazyna Nowak; Fiscal Year: 2013
    ..The goal of this proposal is to determine signaling mechanisms through which two protein kinase C isozymes (PKC-??and PKC-?) regulate mitochondrial dysfunction, oxidative stress, and cell survival during ..
  26. Mechanisms of Ubiquitin Trafficking in Neurons
    Michael C Wooten; Fiscal Year: 2013
    ..as a scaffold to regulate the trafficking of K63-polyubiquitinated proteins and phosphorylation of atypical protein kinase C iota/lambda substrates...
  27. VOLTAGE SENSITIVE SODIUM CHANNELS IN BRAIN
    William A Catterall; Fiscal Year: 2013
    ..We have shown that regulation by protein phosphorylation by cAMP-dependent protein kinase (PKA) and protein kinase C (PKC) requires specific Na channel/kinase signaling complexes and is dependent on regulation of the intrinsic ..
  28. Mediators of Lithium Action in Olfactory Epithelium
    Evaristus A Nwulia; Fiscal Year: 2013
    ..of a large number of genes;notably GSK-32, inositol monophosphatase (IMPase), MIP Synthase, Bcl-2, Akt, Protein Kinase C, MARCKS, insulin-like growth factor binding protein-2 (IGFBP-2), and ERK...
  29. Role of spectrin/ankyrin-G complex in myocyte signaling and cardiac excitability
    Peter J Mohler; Fiscal Year: 2013
    ..Similar to protein kinase A (PKA) and protein kinase C (PKC), CaMKII regulates the phosphorylation status of a host of target substrates throughout the cell...
  30. TNF Alpha in PKC Epsilon Signaling to Carcinogenesis
    Ajit K Verma; Fiscal Year: 2013
    ..by applicant): The objectives of this revised renewal proposal (RO1 CA 102431A2) are to determine whether protein kinase C epsilon (PKC5) signals ultraviolet radiation (UVR)-induced development of squamous cell carcinoma (SCC) via ..
  31. (INTRODUCTION) MECHANISMS OF ACCLIMATIZATION: FETUS AND ADULT
    Lawrence D Longo; Fiscal Year: 2013
    ..g., roles of alphal adrenergic receptor subtypes, protein kinase C isoforms, extracellular signal regulated kinases, and coupling to downstream effectors...
  32. Activation of Atypical PKC by Insulin and Other Agonists
    ROBERT VITO FARESE; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): We have (a) defined upstream activators of atypical protein kinase C (aPKC) and Akt during insulin action in muscle, adipocytes and liver and (b) showed that aPKC is required for insulin-stimulated ..
  33. Spinal Cord-Mediated Acute Neurogenic Inflammation
    Qing Lin; Fiscal Year: 2010
    ..by regulating the functional activity of TRPVi receptors via activating signal transduction cascades, such as protein kinase C (PKC)...
  34. Mammary Gland Morphogenesis and Breast Cancer
    Ian G Macara; Fiscal Year: 2013
    ..self-renewal and differentiation during mammary gland morphogenesis? In the absence of Par3, atypical protein kinase C (aPKC) is mislocalized from the luminal apical surface...
  35. PROTEIN KINASE C IN THE LENS
    DOLORES JEAN TAKEMOTO; Fiscal Year: 2010
    ..In order to prevent hypoxia-driven apoptosis the lens has two stress switch protein kinase C (PKC) isoforms, PKC3 and PKC5...
  36. Regulation of guanylyl cyclase A and B by hormones, ATP and phosphorylation
    Lincoln Ross Potter; Fiscal Year: 2013
    ..In contrast, activated protein kinase C (PKC) is hypothesized to phosphorylate a conserved receptor consensus site that reduces phosphorylation of a ..
  37. Cerebral Artery Alpha1 Adrenergic and PKC Regulatory Mechanisms
    Lawrence D Longo; Fiscal Year: 2013
    ..CA) contractile responses secondary to altered alpha1-adrenergic-receptor (1-AR) subtype and/or specific protein kinase C isoform (PKC)-mediated downstream Ca2+-dependent and Ca2+-independent signal transduction pathways...
  38. Molecular elucidation of the CBM complex in NF-kappaB activation by antigen recep
    Hao Wu; Fiscal Year: 2013
    ..The protein kinase C ? (PKC?) of T-cells and PKC[unreadable] of B-cells are recruited to lipid rafts during antigen-receptor ..
  39. Regulation of cholesterol catabolism by bile acids
    Jongsook Kim Kemper; Fiscal Year: 2013
    ..Surprisingly, preliminary studies have revealed a role for protein kinase C-zeta (PKC?) in regulating SHP activity in response to bile acid signaling by phosphorylating Thr-55 of SHP ..
  40. TGF-BETA, CHLORIDE CHANNELS AND MIGRATION OF EOSINOPHILS
    Devendra K Agrawal; Fiscal Year: 2012
    ..The hypothesis is that TGF-2 activates protein kinase C-4 (PKC-4) to increase voltage-gated chloride current and induces phosphorylation of p38/JNK MAP kinase and ..
  41. Mechanism and functional role of AKAP79/150 in M current control
    Mark S Shapiro; Fiscal Year: 2012
    ..One such AKAP, AKAP79/150 interacts with protein kinase A, protein kinase C (PKC), calmodulin (CaM), calcineurin (CaN), ion channels such as M-type (KCNQ, Kv7) K+ channels, L-type Ca2+ ..
  42. Role of Nox4 in VSMC Migration
    MAZEN KHALIL KHALIL; Fiscal Year: 2011
    ..We will initially focus on integrin- activated, protein kinase C-mediated regulation of the Nox4 binding protein Poldip2...
  43. Regulation of TRPV1 and Nociceptor Sensitization by the Complement System
    Yuriy M Usachev; Fiscal Year: 2013
    ..coupled with TRPV1 in a subset of primary nociceptors;activation of these receptors sensitize nociceptors via protein kinase C-dependent modulation of the TRPV1 channel, which is known to function as a molecular integrator of pain ..
  44. ROLE AND REGULATION OF PROTEIN KINASE C ISOENZYMES
    Yusuf Awni Hannun; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Protein kinase C (PKC) has served as the prototype of lipid-regulated enzymes, especially in the case of the phosphatidylinositol (PI) cycle whereby PI-derived diacylglycerol (DAG) serves as the ..
  45. Role of Endothelin B Receptor in Vascular Protection in Females
    Raouf A Khalil; Fiscal Year: 2011
    ..BP due to downregulation of ETBR expression/activity, and unbalanced activation of ETAR-mediated [Ca2+]I, protein kinase C (PKC) and Rho-kinase pathways of vascular smooth muscle (VSM) contraction...
  46. Chemoprevention of Colorectal Cancer by Aldose Reductase Inhibition
    Satish K Srivastava; Fiscal Year: 2013
    ..that AR-catalyzed reduced products of GS-LDAs, such as GS-DHN, transduce carcinogenic signals downstream to protein kinase C (PKC), the molecular mechanisms that regulate cellular redox homeostasis leading to carcinogenesis are not ..
  47. SHIGA-TOXINS: PRE-CLINICAL ANIMAL MODEL DEVELOPMENT AND THERAPEUTIC TESTING
    Shinichiro Kurosawa; Fiscal Year: 2011
    ..mimetics to neutralize toxins in the circulation and prevent cytotoxicity;and 2) conjugated synthetic protein kinase C peptides that act intracellularly to neutralize toxin-induced signaling pathways that would otherwise lead to ..
  48. Chimaerins-receptors for diacylgly and phorbal esters
    MARCELO GABRIEL KAZANIETZ; Fiscal Year: 2012
    ..ester tumor promoters can trigger responses via direct activation of effectors that do not belong to the protein kinase C (PKC) family, considered until not so long ago the only intracellular receptors for DAG and phorbol esters...
  49. Role of Protein Kinase C in Macrophage Activation
    Michelle R Lennartz; Fiscal Year: 2012
    ..Mice lacking protein kinase C-epsilon (PKC-e) are highly susceptible to bacterial infections and fail to mount an effective inflammatory ..
  50. Glycerolipid synthesis in liver
    ROSALIND ANNE COLEMAN; Fiscal Year: 2013
    ..of phospholipids and TAG, and to regulate nutrient-mediated signaling cascades that involve mTOR, PPAR?, and protein kinase C. To investigate how enhanced TAG synthesis in liver and muscle links to insulin resistance;we will determine ..
  51. Sex-specific fetal programming of adult vascular dysfunction and hypertension
    KUNJU REDDIAR SATHISH KUMAR; Fiscal Year: 2013
    ..postnatal T induces hypertensive responses through sex-specific dysfunctions in vascular smooth muscle (VSM) protein kinase C (PKC) and endothelial EDHF/NO expression/function...
  52. Protein Kinase C Isozymes in Ischemic Heart
    Daria Mochly-Rosen; Fiscal Year: 2013
    ..of the molecular events contributing to cardiac injury by ischemia and reperfusion (I/R) and the role of protein kinase C (PKC) isozymes in this response...
  53. Disease-Modifying Effects of Filgastrim in a Mouse Model of AD
    Juan R Sanchez-Ramos; Fiscal Year: 2012
    ..a novel signaling pathway triggered by G-CSF that involves switching to an alternatively spliced variant of protein kinase C (PKC-delta2) that stimulates hippocampal neurogenesis and inhibits poly- phosphorylation of Tau, both which ..
  54. The role of phosphorylation in regulating the antidiabetic effects of O3FAR-1
    Nader H Moniri; Fiscal Year: 2013
    ..basal phosphorylation of O3FAR1, which regulates receptor expression and agonist sensitivity, is mediated by protein kinase C (PKC), and that there are differential affects of PKC on the two O3FAR1 isoforms...
  55. CAP37 and ocular inflammation
    HELOISE ANNE PEREIRA; Fiscal Year: 2011
    ..in ocular inflammation is dependent on CAP37 and is mediated through the intracellular signaling molecule protein kinase C, we will use techniques that include immunohistochemistry, pharmacological inhibitors, and RNA interference...
  56. Function and regulation of OATP1B1 and OATP1B3
    Wei Yue; Fiscal Year: 2013
    ..results that: 1) OATP1B3 is a phosphorylated protein and the function of OATP1B1 and OATP1B3 is regulated by protein kinase C activation;2) the ubiquitin system is involved in OATP1B1 and OATP1B3 degradation, and proteasome inhibition ..
  57. Central TRPV1 in Cardiovascular Regulation
    MICHAEL CHRISTIAN ANDRESEN; Fiscal Year: 2013
    ..of cannabinoid signaling in afferent activity-dependent generation of asynchronous glutamate release, protein kinase C requirements, G-protein coupled receptor contributions to sensitizing asynchronous release, and NTS TRPV1 ..
  58. Protein Kinase C Signaling Mechanisms in Cancer
    Alan P Fields; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Our long-term goals are to elucidate protein kinase C (PKC) signaling mechanisms that contribute to cancer and translate these mechanistic insights into better prognostic and treatment strategies...
  59. MECHANISMS OF LIVER CELL INJURY
    Gregory J Gores; Fiscal Year: 2013
    ..degradation of Mcl-1 (myeloid cell leukemia-1), a potent antiapoptotic Bcl-2 protein: a) by a PKC8 (protein kinase C 8)-dependent proteasome degradation pathway;and b) is essential for rapid induction of lipoapoptosis...
  60. Cellular Mechanisms of Amniotic Fluid Volume Regulation
    Cecilia Y Cheung; Fiscal Year: 2013
    ..The involvement of other signaling proteins including protein kinase C and phosphatidylinositol 3-kinase will be explored...
  61. Propofol and Protein Kinase C: Molecular Interactions in Cardiomyocytes
    DEREK SCOTT DAMRON; Fiscal Year: 2011
    ..Activation of protein kinase C epsilon (PKCe) has been shown to play a key role in mediating anesthesia-induced myocardial protection, ..
  62. Alcohol increases tight junction permeability &disrupts translocation of ZO-1
    Samantha Simet; Fiscal Year: 2012
    ..Our data link this "leakiness" to alcohol-induced changes in airway protein kinase C (PKC) activation resulting in impaired protein translocation to the cell membrane...
  63. ADENOSINE AND ANTIOXIDANT ENZYMES
    Vickram Ramkumar; Fiscal Year: 1999
    ..A23187 (a Ca2+ ionophore) and phorbol esters (activators of protein kinase C) both mimicked activation of these enzymes via the A3AR...
  64. MOLECULAR BASIS FOR DRUG INDUCED CARDIOTOXICITY IN AIDS
    CRAIG CLARKSON; Fiscal Year: 2001
    ..2) the effects of inflammatory cytokines on human cardiac ion channels are mediated by activation of protein kinase C; 3) cocaine and its major metabolites, as well as selected antimicrobial drugs block HERG-encoded K channels ..
  65. PROTEIN KINASE C AND PHOTORECEPTORS
    ALEXANDRA NEWTON; Fiscal Year: 2002
    The long term goal of the proposed research if to elucidate the role of protein kinase C in rod outer segments of photoreceptors...
  66. PHOSPHOLIPASE C/PROTEIN KINASE C IN PSORIATIC EPIDERMIS
    Gary Fisher; Fiscal Year: 1993
    ..Many of these mediators exert their effects by activation of the phospholipase C/protein kinase C signal transduction system...
  67. HUMAN LYMPHOCYTE PRODUCTION OF TUMOR NECROSIS FACTOR
    Sun Sang Sung; Fiscal Year: 1993
    ..In particular, the relationship between protein kinase C activation, Ca2+ mobilization and cAMP increases and TNF mRNA and cytokine syntheses in B and T lymphocytes ..
  68. MYOCARDIAL PROTEIN KINASE C & B-ADRENOCEPTORS IN CHF
    Chang Seng Liang; Fiscal Year: 1990
    b>Protein kinase C, a Ca2+- and phospholipid-dependent protein phosphorylation enzyme present in myocardium, has been shown recently to induce cardiac beta-adrenergic desensitization by phosphorylation of beta-adrenoceptors...
  69. PROTEIN KINASE C MEDIATION OF EXOCRINE PANCREATIC SECRET
    Dexter Louie; Fiscal Year: 1992
    ..IP3 serves to mobilize intracellular calcium and DAG activates protein kinase C (PKC). The production of these compounds has been proposed to mediate pancreatic amylase secretion...