creatine kinase


Summary: A transferase that catalyzes formation of PHOSPHOCREATINE from ATP + CREATINE. The reaction stores ATP energy as phosphocreatine. Three cytoplasmic ISOENZYMES have been identified in human tissues: the MM type from SKELETAL MUSCLE, the MB type from myocardial tissue and the BB type from nervous tissue as well as a mitochondrial isoenzyme. Macro-creatine kinase refers to creatine kinase complexed with other serum proteins.

Top Publications

  1. pmc Objective assessment of reduced invasiveness in MED. Compared with conventional one-level laminotomy
    Ryuichi Sasaoka
    Department of Orthopaedic Surgery, Osaka City University Medical School, Osaka 545 8585, Japan
    Eur Spine J 15:577-82. 2006
  2. pmc Conformational change in the C-terminal domain is responsible for the initiation of creatine kinase thermal aggregation
    Hua Wei He
    Department of Biological Sciences and Biotechnology, and State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China
    Biophys J 89:2650-8. 2005
  3. pmc New aspects on patients affected by dysferlin deficient muscular dystrophy
    Lars Klinge
    University of Newcastle, Institute of Human Genetics, The Institute of Human Genetics, International Centre for Life, Central Parkway, Newcastle upon Tyne, UK
    J Neurol Neurosurg Psychiatry 81:946-53. 2010
  4. ncbi Elevations of creatine kinase in patients treated with imatinib mesylate (Gleevec)
    Jessica K Gordon
    Department of Rheumatology, Hospital for Special Surgeryy, New York, NY 10021, USA
    Leuk Res 34:827-9. 2010
  5. ncbi New insights into doxorubicin-induced cardiotoxicity: the critical role of cellular energetics
    Malgorzata Tokarska-Schlattner
    Institute of Cell Biology, ETH Zurich, Hönggerberg HPM D24, CH 8093 Zurich, Switzerland
    J Mol Cell Cardiol 41:389-405. 2006
  6. ncbi The failing heart--an engine out of fuel
    Stefan Neubauer
    Department of Cardiovascular Medicine, University of Oxford and John Radcliffe Hospital, Oxford, United Kingdom
    N Engl J Med 356:1140-51. 2007
  7. pmc The creatine kinase system and pleiotropic effects of creatine
    Theo Wallimann
    Institute of Cell Biology, ETH Zurich, Zurich, Switzerland
    Amino Acids 40:1271-96. 2011
  8. ncbi Mesoangioblast stem cells ameliorate muscle function in dystrophic dogs
    Maurilio Sampaolesi
    San Raffaele Scientific Institute, Universita Vita e Salute, Stem Cell Research Institute, Via Olgettina 58, 20132 Milan, Italy
    Nature 444:574-9. 2006
  9. ncbi Statin-associated myopathy
    Paul D Thompson
    Preventive Cardiology and Cardiovascular Research, Division of Cardiology, Hartford Hospital, Hartford, Conn 06102, USA
    JAMA 289:1681-90. 2003
  10. ncbi Serum creatine kinase levels and renal function measures in exertional muscle damage
    Priscilla M Clarkson
    Department of Exercise Science, University of Massachusetts, Amherst, MA 01003, USA
    Med Sci Sports Exerc 38:623-7. 2006

Detail Information

Publications391 found, 100 shown here

  1. pmc Objective assessment of reduced invasiveness in MED. Compared with conventional one-level laminotomy
    Ryuichi Sasaoka
    Department of Orthopaedic Surgery, Osaka City University Medical School, Osaka 545 8585, Japan
    Eur Spine J 15:577-82. 2006
    ..However, an indicator for muscle damage (CPK) appeared not to be affected by the type of surgical procedure used to correct disc herniation...
  2. pmc Conformational change in the C-terminal domain is responsible for the initiation of creatine kinase thermal aggregation
    Hua Wei He
    Department of Biological Sciences and Biotechnology, and State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China
    Biophys J 89:2650-8. 2005
    ..the conformational change that is responsible for the fast destabilization and aggregation of rabbit muscle creatine kinase (EC induced by heat was studied by intrinsic fluorescence and infrared spectroscopy...
  3. pmc New aspects on patients affected by dysferlin deficient muscular dystrophy
    Lars Klinge
    University of Newcastle, Institute of Human Genetics, The Institute of Human Genetics, International Centre for Life, Central Parkway, Newcastle upon Tyne, UK
    J Neurol Neurosurg Psychiatry 81:946-53. 2010
  4. ncbi Elevations of creatine kinase in patients treated with imatinib mesylate (Gleevec)
    Jessica K Gordon
    Department of Rheumatology, Hospital for Special Surgeryy, New York, NY 10021, USA
    Leuk Res 34:827-9. 2010
    ..Although muscular complaints are common, elevations of creatine kinase (CK) are listed as <1% in the product label...
  5. ncbi New insights into doxorubicin-induced cardiotoxicity: the critical role of cellular energetics
    Malgorzata Tokarska-Schlattner
    Institute of Cell Biology, ETH Zurich, Hönggerberg HPM D24, CH 8093 Zurich, Switzerland
    J Mol Cell Cardiol 41:389-405. 2006
  6. ncbi The failing heart--an engine out of fuel
    Stefan Neubauer
    Department of Cardiovascular Medicine, University of Oxford and John Radcliffe Hospital, Oxford, United Kingdom
    N Engl J Med 356:1140-51. 2007
  7. pmc The creatine kinase system and pleiotropic effects of creatine
    Theo Wallimann
    Institute of Cell Biology, ETH Zurich, Zurich, Switzerland
    Amino Acids 40:1271-96. 2011
    The pleiotropic effects of creatine (Cr) are based mostly on the functions of the enzyme creatine kinase (CK) and its high-energy product phosphocreatine (PCr)...
  8. ncbi Mesoangioblast stem cells ameliorate muscle function in dystrophic dogs
    Maurilio Sampaolesi
    San Raffaele Scientific Institute, Universita Vita e Salute, Stem Cell Research Institute, Via Olgettina 58, 20132 Milan, Italy
    Nature 444:574-9. 2006
    ..The outcome is a remarkable clinical amelioration and preservation of active motility. These data qualify mesoangioblasts as candidates for future stem cell therapy for Duchenne patients...
  9. ncbi Statin-associated myopathy
    Paul D Thompson
    Preventive Cardiology and Cardiovascular Research, Division of Cardiology, Hartford Hospital, Hartford, Conn 06102, USA
    JAMA 289:1681-90. 2003
    ..with skeletal muscle complaints, including clinically important myositis and rhabdomyolysis, mild serum creatine kinase (CK) elevations, myalgia with and without elevated CK levels, muscle weakness, muscle cramps, and persistent ..
  10. ncbi Serum creatine kinase levels and renal function measures in exertional muscle damage
    Priscilla M Clarkson
    Department of Exercise Science, University of Massachusetts, Amherst, MA 01003, USA
    Med Sci Sports Exerc 38:623-7. 2006
    Serum creatine kinase (CK) levels are commonly used to judge the severity of muscle damage and to determine when to hospitalize patients who present with symptoms of exertional rhabdomyolysis in order to prevent renal failure...
  11. pmc Energy metabolism in heart failure and remodelling
    Joanne S Ingwall
    NMR Laboratory for Physiological Chemistry, Division of Cardiovascular Medicine, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, 221 Longwood Avenue, Room 247, Boston, MA 02115, USA
    Cardiovasc Res 81:412-9. 2009
    ..The time line for changes in the pathways for ATP synthesis in compensated hypertrophy is: flux through the creatine kinase (CK) reaction falls as both creatine concentration ([Cr]) and CK activity fall; increases in [ADP] and [AMP] ..
  12. ncbi Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle
    D Yaffe
    Nature 270:725-7. 1977
  13. ncbi Marked reduction of Na(+), K(+)-ATPase and creatine kinase activities induced by acute lysine administration in glutaryl-CoA dehydrogenase deficient mice
    Alexandre Umpierrez Amaral
    Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
    Mol Genet Metab 107:81-6. 2012
    ..activities of the respiratory chain complexes II, II-III and IV, α-ketoglutarate dehydrogenase (α-KGDH), creatine kinase (CK) and synaptic Na(+), K(+)-ATPase...
  14. pmc Reference intervals for serum creatine kinase in athletes
    Vassilis Mougios
    TEFAA, University of Thessaloniki, 541 24 Thessaloniki, Greece
    Br J Sports Med 41:674-8. 2007
    The serum concentration of creatine kinase (CK) is used widely as an index of skeletal muscle fibre damage in sport and exercise...
  15. ncbi Expression and carbonylation of creatine kinase in the quadriceps femoris muscles of patients with chronic obstructive pulmonary disease
    Esther Barreiro
    Critical Care and Respiratory Divisions, Royal Victoria Hospital and Meakins Christie Laboratories, McGill University, Room L3 05, 687 Pine Avenue West, Montreal, PQ, H3A 1A1 Canada
    Am J Respir Cell Mol Biol 33:636-42. 2005
    ..Both creatine kinase (CK) and carbonic anhydrase III (CAIII) were identified as being strongly carbonylated in this muscle in both ..
  16. pmc Modulation of energy transfer pathways between mitochondria and myofibrils by changes in performance of perfused heart
    Marko Vendelin
    Laboratory of Systems Biology, Institute of Cybernetics, Tallinn University of Technology, Akadeemia 21, 12618 Tallinn, Estonia
    J Biol Chem 285:37240-50. 2010
    ..This process is mediated both by the creatine kinase (CK) shuttle and by direct ATP transfer...
  17. pmc Immunological changes in human skeletal muscle and blood after eccentric exercise and multiple biopsies
    C Malm
    Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden
    J Physiol 529:243-62. 2000
    ..4. Delayed onset muscle soreness, serum creatine kinase activity and C-reactive protein concentration were not related to leukocyte infiltration in human skeletal ..
  18. ncbi Green tea extract supplementation gives protection against exercise-induced oxidative damage in healthy men
    Ewa Jówko
    Department of Biochemistry, Faculty of Physical Education and Sport in Biala Podlaska, Jozef Pilsudski University of Physical Education in Warsaw, Akademicka 2, 21 500 Biala Podlaska, Poland
    Nutr Res 31:813-21. 2011
    ..In term I, the muscular endurance test induced an increase in activity of creatine kinase in plasma after 24 hours of recovery in both the P and GTE groups...
  19. ncbi Daptomycin exposure and the probability of elevations in the creatine phosphokinase level: data from a randomized trial of patients with bacteremia and endocarditis
    Sujata M Bhavnani
    Institute for Clinical Pharmacodynamics, Ordway Research Institute, Albany, New York, USA
    Clin Infect Dis 50:1568-74. 2010
  20. ncbi Postconditioning attenuates myocardial ischemia-reperfusion injury by inhibiting events in the early minutes of reperfusion
    Hajime Kin
    Department of Cardiothoracic Surgery, The Carlyle Fraser Heart Center Crawford Long Hospital, Emory University School of Medicine, Atlanta, GA 30308 2225, USA
    Cardiovasc Res 62:74-85. 2004
    ..This study tested the hypothesis that the early minutes of reperfusion (R) during which postconditioning (Post-con) is applied are critical to its cardioprotection...
  21. ncbi A novel approach for screening the proteome for changes in protein conformation
    Anson Pierce
    Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229, USA
    Biochemistry 45:3077-85. 2006
    ..cytosol that exhibited a marked loss of incorporation of BisANS after exposure to in vitro oxidative stress: creatine kinase (CK) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH)...
  22. ncbi Evidence for a synergistic action of glutaric and 3-hydroxyglutaric acids disturbing rat brain energy metabolism
    Gustavo C Ferreira
    Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
    Int J Dev Neurosci 25:391-8. 2007
    ..production from labeled glucose and acetate, as well as the activities of pyruvate dehydrogenase and creatine kinase. We first observed that glutaric (5 mM), 3-hydroxyglutaric (1 mM) and quinolinic acids (0...
  23. ncbi Palmatine from Coptidis rhizoma reduces ischemia-reperfusion-mediated acute myocardial injury in the rat
    Young Min Kim
    Department of Pharmacology, School of Medicine, and Institute of Health Sciences, Gyeongsang National University, 92 Chilamdong, Jinju, Republic of Korea
    Food Chem Toxicol 47:2097-102. 2009
    ..We concluded that palmatine protects hearts from I/R injury in rats possibly by reducing oxidative stress and modulating inflammatory mediators...
  24. ncbi Glutaric acid moderately compromises energy metabolism in rat brain
    Gustavo da C Ferreira
    Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600 Anexo, Porto Alegre, RS, Brazil
    Int J Dev Neurosci 23:687-93. 2005
    ..activities of energy metabolism, namely the respiratory chain complexes I-IV, succinate dehydrogenase and creatine kinase in midbrain of developing rats...
  25. ncbi Proteomic identification of oxidatively modified proteins in Alzheimer's disease brain. Part I: creatine kinase BB, glutamine synthase, and ubiquitin carboxy-terminal hydrolase L-1
    Alessandra Castegna
    Department of Chemistry, Center of Membrane Sciences, University of Kentucky, Lexington 40506 0055, USA
    Free Radic Biol Med 33:562-71. 2002
    ..death in AD, and was partially achieved previously in our laboratory through immunochemical detection of creatine kinase BB and beta-actin as specifically oxidized proteins in AD brain versus control brain...
  26. ncbi 'Postconditioning' the human heart: multiple balloon inflations during primary angioplasty may confer cardioprotection
    Chad E Darling
    Dept of Emergency Medicine, University of Massachusetts Medical School, 55 Lake Avenue, North Worcester, MA, 01655, USA
    Basic Res Cardiol 102:274-8. 2007
    ..Using a retrospective study design, our aim was to test the hypothesis that creatine kinase release (CK: clinical surrogate of infarct size) would be attenuated in ST-segment elevation myocardial ..
  27. ncbi Myopathy and neuropathy associated with nucleos(t)ide analog therapy for hepatitis B
    Russell D Fleischer
    Division of Antiviral Products, Center for Drug Evaluation and Research, Food and Drug Administration, Silver Spring, MD, USA
    J Hepatol 51:787-91. 2009
  28. pmc Moderate exercise improves leucocyte function and decreases inflammation in diabetes
    M F Belotto
    Cruzeiro do Sul University, Brazil
    Clin Exp Immunol 162:237-43. 2010
    ..Serum activities of creatine kinase (CK) and lactate dehydrogenase (LDH) were not modified in the conditions studied...
  29. pmc Probucol attenuates cyclophosphamide-induced oxidative apoptosis, p53 and Bax signal expression in rat cardiac tissues
    Yosef A Asiri
    Department of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh, Kingdom of Saudi Arabia
    Oxid Med Cell Longev 3:308-16. 2010
  30. pmc Flow of energy in the outer retina in darkness and in light
    Jonathan D Linton
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 107:8599-604. 2010
    ..Consistent with this model, inhibition of creatine kinase in avascular retinas blocks synaptic transmission without influencing outer segment activity...
  31. pmc Oxidative modification and aggregation of creatine kinase from aged mouse skeletal muscle
    Jonathan E Nuss
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555 0643, USA
    Aging (Albany NY) 1:557-72. 2009
    b>Creatine kinase catalyzes the reversible transfer of the gamma phosphate from ATP to creatine forming the high energy compound creatine phosphate...
  32. ncbi Rhabdomyolysis
    W H Bagley
    Department of Emergency Medicine, St Luke s Roosevelt Hospital, University Hospital of Columbia Physicians and Surgeons, New York, NY 10025, USA
    Intern Emerg Med 2:210-8. 2007
    ..Irrespective of the cause of rhabdomyolysis the mortality rate may still be as high as 8%. This is a comprehensive review of the pathophysiology, diagnosis, complications and treatment options for rhabdomyolysis...
  33. ncbi Functional improvement of dystrophic muscle by myostatin blockade
    Sasha Bogdanovich
    Department of Physiology and Pennsylvania Muscle Institute, University of Pennsylvania School of Medicine, 3700 Hamilton Walk, Richards A 601, Philadelphia, Pennsylvania 19104 6085, USA
    Nature 420:418-21. 2002 mdx mouse muscle along with a significant decrease in muscle degeneration and concentrations of serum creatine kinase. The functional improvement of dystrophic muscle by myostatin blockade provides a novel, pharmacological ..
  34. ncbi Plasma 99th percentile reference limits for cardiac troponin and creatine kinase MB mass for use with European Society of Cardiology/American College of Cardiology consensus recommendations
    Fred S Apple
    Department of Laboratory Medicine and Pathology, Hennepin County Medical Center and the University of Minnesota School of Medicine, 701 Park Avenue, Minneapolis, MN 55415, USA
    Clin Chem 49:1331-6. 2003
    ..document for definition of myocardial infarction (MI) is predicated on increased cardiac troponin or creatine kinase (CK) MB mass above the 99th percentile reference limit...
  35. ncbi Rhabdomyolysis: a review of the literature
    F Y Khan
    Department of Medicine, Hamad General Hospital, Doha, Qatar
    Neth J Med 67:272-83. 2009
    ..An elevated plasma creatine kinase (CK) level is the most sensitive laboratory finding pertaining to muscle injury; whereas hyperkalaemia, acute ..
  36. pmc Eccentric exercise-induced morphological changes in the membrane systems involved in excitation-contraction coupling in rat skeletal muscle
    H Takekura
    Department of Physiological Sciences, National Institute of Fitness and Sports, Kanoya, Kagoshima 891 2393, Japan
    J Physiol 533:571-83. 2001
    ..These observations also support the idea that eccentric exercise- induced E-C coupling failure is due to physical and chemical disruption of the membrane systems involved in the E-C coupling process in skeletal muscle...
  37. ncbi Functions and effects of creatine in the central nervous system
    Robert H Andres
    Department of Neurosurgery, University of Berne, Inselspital, CH 3010 Berne, Switzerland
    Brain Res Bull 76:329-43. 2008
    b>Creatine kinase catalyses the reversible transphosphorylation of creatine by ATP...
  38. ncbi Phenotypic study in 40 patients with dysferlin gene mutations: high frequency of atypical phenotypes
    Karine Nguyen
    Departement de Genetique Medicale, Hopital Timone, Marseille, France
    Arch Neurol 64:1176-82. 2007
    ..To describe the phenotypic spectrum of dysferlin (DYSF) gene mutations (which cause dysferlinopathies, autosomal recessive muscular dystrophies) in patients with a dysferlin protein deficiency...
  39. ncbi Creatine kinase isoenzymes in spermatozoa
    T Wallimann
    J Muscle Res Cell Motil 7:25-34. 1986
    Two isoforms of creatine kinase (CK, E.C., the brain type BB-CK and the mitochondrial-bound MiMi-CK, as well as adenylate kinase (myokinase, E.C. 2.7.4...
  40. ncbi Muscle creatine kinase-deficient mice. I. Alterations in myofibrillar function
    R Ventura-Clapier
    Laboratoire de Cardiologie Cellulaire et Moleculaire, CJF INSERM 92 11, Faculte de Pharmacie, Universite Paris Sud, Chatenay Malabry, France
    J Biol Chem 270:19914-20. 1995
    The regulation of contractile activity in mice bearing a null mutation of the M-isoform of creatine kinase gene, has been investigated in tissue extracts and Triton X-100-treated preparations of ventricular, soleus, and gastrocnemius ..
  41. ncbi Peroxynitrite induced nitration and inactivation of myofibrillar creatine kinase in experimental heart failure
    M J Mihm
    Division of Pharmacology/College of Pharmacy and OSU Heart and Lung Research Institute The Ohio State University, 412 Riffe Building, 500 West Twelfth Street, Columbus, OH 43210, USA
    Cardiovasc Res 49:798-807. 2001
    ..peroxynitrite (ONOO(-), an aggressive biological oxidant and nitrating agent) potently inhibits myofibrillar creatine kinase (MM-CK), a critical controller of contractility known to be impaired during heart failure...
  42. ncbi Preventing renal failure in patients with rhabdomyolysis: do bicarbonate and mannitol make a difference?
    Carlos V R Brown
    Department of Surgery, Division of Trauma and Critical Care, University of Southern California and the Los Angeles County Medical Center, Los Angeles, California, USA
    J Trauma 56:1191-6. 2004
    ..The purpose of this study was to determine whether BIC/ MAN is effective in preventing RF in patients with rhabdomyolysis caused by trauma...
  43. ncbi Structural and behavioural consequences of double deficiency for creatine kinases BCK and UbCKmit
    Femke Streijger
    Department of Cell Biology, NCMLS, UMC Radboud, University of Nijmegen, P O Box 9101, 6500 HB Nijmegen, The Netherlands
    Behav Brain Res 157:219-34. 2005
    The cytosolic brain-type creatine kinase (BCK) isoform and the mitochondrial ubiquitous creatine kinase (UbCKmit) isoform are both important for the maintenance and distribution of cellular energy in neurons and astrocytes...
  44. ncbi Comparative MRI analysis of T2 changes associated with single and repeated bouts of downhill running leading to eccentric-induced muscle damage
    Tanguy Marqueste
    Centre de Resonance Magnetique Biologique et Medicale, Unite Mixte de Recherche UMR Centre National de la Recherche Scientifique CNRS 6612, Faculte de Medecine de Marseille, Marseille, France
    J Appl Physiol (1985) 105:299-307. 2008
    ..To clarify this issue, we measured blood creatine kinase and lactate dehydrogenase activities and proton transverse relaxation time (T2) in various leg muscles 72 h ..
  45. ncbi Taurine supplementation decreases oxidative stress in skeletal muscle after eccentric exercise
    Luciano A Silva
    Exercise Biochemistry and Physiology Laboratory, Postgraduate Program in Health Sciences, Health Sciences Unit, Universidade do Extremo Sul Catarinense, Criciuma, SC, Brazil
    Cell Biochem Funct 29:43-9. 2011
    ..Production of superoxide anion, creatine kinase (CK) levels, lipoperoxidation, carbonylation, total thiol content and antioxidant enzyme were analysed...
  46. ncbi Long-term benefit of postconditioning
    Helene Thibault
    Hospices Civils de Lyon, Universite Claude Bernard Lyon 1, Lyon, France
    Circulation 117:1037-44. 2008
    We previously demonstrated that ischemic postconditioning decreases creatine kinase release, a surrogate marker for infarct size, in patients with acute myocardial infarction...
  47. ncbi Effect of hydrotherapy on the signs and symptoms of delayed onset muscle soreness
    Joanna Vaile
    Department of Physiology, Australian Institute of Sport, PO Box 176, Belconnen, ACT, Australia
    Eur J Appl Physiol 102:447-55. 2008
    ..While HWI was effective in the recovery of isometric force, it was ineffective for recovery of all other markers compared to PAS...
  48. ncbi Relation of temporal creatine kinase-MB release and outcome after thrombolytic therapy for acute myocardial infarction. TAMI Study Group
    R H Christenson
    Department of Pathology, University of Maryland School of Medicine, Baltimore
    Am J Cardiol 85:543-7. 2000
    ..We sought to relate in-hospital outcomes and curve-fitted creatine kinase (CK)-MB variables after thrombolysis...
  49. ncbi Creatine kinase monitoring in sport medicine
    Paola Brancaccio
    Department of Experimental Medicine Sport Medicine, Centre of Excellence of Cardiovascular Disease, Seconda Universita di Napoli, Napoli, Italy
    Br Med Bull 81:209-30. 2007
    AREAS OF GENERAL AGREEMENT: Total creatine kinase (CK) levels depend on age, gender, race, muscle mass, physical activity and climatic condition...
  50. pmc The SLCO1B1*5 genetic variant is associated with statin-induced side effects
    Deepak Voora
    Division of Cardiovascular Medicine, Duke University Medical Center, Durham, North Carolina 27708, USA
    J Am Coll Cardiol 54:1609-16. 2009
    ..We sought to identify single nucleotide polymorphisms associated with mild statin-induced side effects...
  51. pmc High-energy phosphotransfer in the failing mouse heart: role of adenylate kinase and glycolytic enzymes
    Dunja Aksentijevic
    Department of Cardiovascular Medicine, Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK
    Eur J Heart Fail 12:1282-9. 2010
    To measure the activity of the key phosphotransfer enzymes creatine kinase (CK), adenylate kinase (AK), and glycolytic enzymes in two common mouse models of chronic heart failure.
  52. pmc Impaired ATP kinetics in failing in vivo mouse heart
    Ashish Gupta
    Department of Medicine, Division of Cardiology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Circ Cardiovasc Imaging 4:42-50. 2011
    ..supported in part by observations of reduced rates of adenosine 5'-triphosphate (ATP) synthesis through the creatine kinase (CK) reaction, the primary myocardial energy reservoir, in patients with heart failure (HF)...
  53. ncbi Delayed-onset muscle soreness does not reflect the magnitude of eccentric exercise-induced muscle damage
    Kazunori Nosaka
    Exercise and Sports Science, Graduate School of Integrated Science, Yokohama City University, Yokohama, Japan
    Scand J Med Sci Sports 12:337-46. 2002
    ..Maximal isometric force, relaxed and flexed elbow joint angles, upper arm circumference, and plasma creatine kinase activity were assessed immediately before and after, and for 4 days after exercise...
  54. ncbi MyoD is a sequence-specific DNA binding protein requiring a region of myc homology to bind to the muscle creatine kinase enhancer
    A B Lassar
    Department of Genetics, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
    Cell 58:823-31. 1989 that MyoD is a DNA binding protein capable of specific interaction with two regions of the mouse muscle creatine kinase gene upstream enhancer, both of which are required for full muscle-specific enhancer activity...
  55. pmc Triple repetition time saturation transfer (TRiST) 31P spectroscopy for measuring human creatine kinase reaction kinetics
    Michael Schar
    Russell H Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
    Magn Reson Med 63:1493-501. 2010
    Human cardiac phosphorus MR saturation transfer experiments to quantify creatine kinase forward rate constants (k(f)) have previously been performed at 1.5 T...
  56. ncbi Prognostic value, kinetics and effect of CVVHDF on serum of the myoglobin and creatine kinase in critically ill patients with rhabdomyolysis
    T S Mikkelsen
    Department of Intensive Care, Aarhus University Hospital, Aarhus, Denmark
    Acta Anaesthesiol Scand 49:859-64. 2005
    I) To investigate the kinetics of the myoglobin and creatine kinase (CK) in rhabdomyolysis. Especially to describe those patients in whom an isolated increase in the myoglobin or the CK occurred at a later stage...
  57. ncbi Serum creatine kinase as predictor of clinical course in rhabdomyolysis: a 5-year intensive care survey
    Arthur R de Meijer
    Department of Intensive Care, Meander Medical Centre, P O Box 1502, 3800 BM, Amersfoort, The Netherlands
    Intensive Care Med 29:1121-5. 2003
    ..To evaluate the risk factors for the development of acute renal failure (ARF) in severe rhabdomyolysis...
  58. ncbi Effects of polyphenolic antioxidants on exercise-induced oxidative stress
    J M Morillas-Ruiz
    E U Human and Dietetic Nutrition, San Antonio Catholic University, Campus Los Jerónimos, s n 30107, Murcia
    Clin Nutr 25:444-53. 2006
    ..antioxidant status (TAS); protein oxidation (carbonyl groups, CO) and the lactate dehydrogenase (LDH) and creatine kinase (CK) enzymes for each trial. All values were adjusted for changes in plasma volume...
  59. pmc Targeting of the creatine kinase M gene in embryonic stem cells using isogenic and nonisogenic vectors
    J van Deursen
    Department of Cell Biology and Histology, Faculty of Medical Sciences, University of Nijmegen, The Netherlands
    Nucleic Acids Res 20:3815-20. 1992
    ..genomic DNA originating from different mouse strains were used to introduce site-specific mutations into the creatine kinase M (CKM) gene of mouse embryonic stem (ES) cells...
  60. pmc Four-angle saturation transfer (FAST) method for measuring creatine kinase reaction rates in vivo
    Paul A Bottomley
    Division of MR Research, Department of Radiology, Johns Hopkins University, Baltimore, Maryland 21287 0843, USA
    Magn Reson Med 47:850-63. 2002
    ..Potential errors in the rate measurements due to the effects of exchange are evaluated for creatine kinase (CK) metabolism modeled for skeletal and heart muscle, and are found to be < 5% for forward CK flux rates ..
  61. ncbi active site modifications of the brain isoform of creatine kinase by 4-hydroxy-2-nonenal correlate with reduced enzyme activity: mapping of modified sites by Fourier transform-ion cyclotron resonance mass spectrometry
    Shannon M Eliuk
    Department of Pharmacology Toxicology, UAB Biomedical FT ICR MS Laboratory, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, LA 35294, USA
    Chem Res Toxicol 20:1260-8. 2007
    b>Creatine kinase reversibly catalyzes the transfer of the high-energy phosphoryl group from phosphocreatine to MgADP for rapid regeneration of ATP...
  62. ncbi Causes of acute renal dysfunction after percutaneous coronary intervention and comparison of late mortality rates with postprocedure rise of creatine kinase-MB versus rise of serum creatinine
    Joseph Lindsay
    Division of Cardiology, Washington Hospital Center, 110 Irving Street NW, Washington, DC 20010, USA
    Am J Cardiol 94:786-9. 2004
    Increases in both serum creatinine and creatine kinase-MB (CK-MB) after percutaneous coronary intervention are associated with increased risk for late adverse cardiovascular events...
  63. ncbi Reduced albumin-cobalt binding with transient myocardial ischemia after elective percutaneous transluminal coronary angioplasty: a preliminary comparison to creatine kinase-MB, myoglobin, and troponin I
    D Bar-Or
    Swedish Medical Center
    Am Heart J 141:985-91. 2001
    ..We then compared ACB assay results with creatine kinase isoenzyme (CK-MB), myoglobin, and cardiac troponin I (cTn-I) values after PTCA.
  64. ncbi Ankyrin repeat and suppressors of cytokine signaling box protein asb-9 targets creatine kinase B for degradation
    Marlyse A Debrincat
    Division of Cancer and Haematology, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
    J Biol Chem 282:4728-37. 2007
    ..Using a proteomics approach, we demonstrate that creatine kinase B (CKB) interacts with Asb-9 in a specific, SOCS box-independent manner...
  65. ncbi Tongxinluo reduces myocardial no-reflow and ischemia-reperfusion injury by stimulating the phosphorylation of eNOS via the PKA pathway
    Xiang Dong Li
    Department of Cardiology, Fuwai Hospital and Cardiovascular Institute, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China
    Am J Physiol Heart Circ Physiol 299:H1255-61. 2010
    ..TXL decreased creatine kinase (CK) activity (P < 0.05) and reduced the no-reflow area from 48.6% to 9.5% and infarct size from 78...
  66. ncbi Cocoa-based protein and carbohydrate drink decreases perceived soreness after exhaustive aerobic exercise: a pragmatic preliminary analysis
    Nicole M McBrier
    Department of Kinesiology, The Pennsylvania State University, University Park, Pennsylvania, USA
    J Strength Cond Res 24:2203-10. 2010
    ..Common biomarkers indicative of skeletal muscle damage included creatine kinase (CK), urinary isoprostanes and inflammatory markers (IL-6, IL-8, C-Reactive Protein [CRP])...
  67. ncbi Reference change values of blood analytes from physically active subjects
    Lázaro Alessandro S Nunes
    Departamento de Bioquimica, Laboratório de Bioquímica do Exercício LABEX, Instituto de Biologia, Universidade de Campinas UNICAMP, Cidade Universitaria Zeferino Vaz, S N, Barao Geraldo, Campinas, SP, CEP 13083 970, Brazil
    Eur J Appl Physiol 110:191-8. 2010
    ..Blood samples (10 mL) were collected monthly from 56 male subjects (17-19 years old). Creatine kinase and aspartate aminotransferase activities, total cholesterol, triglycerides, uric acid, C-reactive protein, ..
  68. ncbi Acute effects of chocolate milk and a commercial recovery beverage on postexercise recovery indices and endurance cycling performance
    Kelly Pritchett
    Department of Health, Human Performance, and Nutrition, Central Washington University, Ellensburg, WA 98926, USA
    Appl Physiol Nutr Metab 34:1017-22. 2009
    ..2 min, CRB 13.5 +/- 8.9 min). The change in creatine kinase (CK) was significantly (p < 0.05) greater in the CRB trial than in the CHOC trial (increase CHOC 27...
  69. ncbi Influenza B-associated rhabdomyolysis in Taiwanese children
    C T Wu
    Division of Pediatric Emergency Medicine, Chang Gung Children s Hospital, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Tauyuan, Taiwan
    Acta Paediatr 99:1701-4. 2010
    ..The aim of this study was to describe the clinical and laboratory manifestations, complications and outcomes of IBAR in Taiwanese children...
  70. ncbi Incomplete filling of lithium heparin tubes affects the activity of creatine kinase and gamma-glutamyltransferase
    G Lippi
    U O Diagnostica Ematochimica, Azienda Ospedaliero Universitaria di Parma, Italy
    Br J Biomed Sci 69:67-70. 2012 assess whether or not incomplete filling of primary lithium heparin tubes may influence the activity of creatine kinase (CK), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyltransferase (GGT)...
  71. ncbi Changes in selected biochemical, muscular strength, power, and endurance measures during deliberate overreaching and tapering in rugby league players
    A Coutts
    School of Leisure, Sport and Tourism, University of Technology, Kuring gai Campus, Lindfield, NSW 2070, Sydney, Australia
    Int J Sports Med 28:116-24. 2007
    ..the plasma testosterone to cortisol ratio, plasma glutamate, plasma glutamine to glutamate ratio and plasma creatine kinase activity demonstrated significant changes at the end of the overload training period (p < 0.05)...
  72. ncbi Gross ultrastructural changes and necrotic fiber segments in elbow flexor muscles after maximal voluntary eccentric action in humans
    Fredrik Lauritzen
    Department of Anatomy and Centre for Molecular Biology and Neuroscience, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
    J Appl Physiol (1985) 107:1923-34. 2009
    ..The present study demonstrates that the more severe end of the continuum of ultrastructural changes occurs in humans after voluntary exercise when maximal eccentric muscle actions are involved...
  73. ncbi Cardiac troponin I, cardiac troponin T and creatine kinase MB concentrations in umbilical cord blood of healthy term neonates
    D Trevisanuto
    Paediatric Department, Medical School, University of Padua, Azienda Ospedaliera di Padova, Padua, Italy
    Acta Paediatr 92:1463-7. 2003
    To measure and compare cardiac troponin I, cardiac troponin T and creatine kinase MB concentrations in the umbilical cord blood of healthy term infants and to investigate the relationship between maternal and neonatal troponin values at ..
  74. ncbi Break point of serum creatine kinase release after endurance exercise
    Manabu Totsuka
    Department of Health and Physical Education, Faculty of Education, Hirosaki University, Hirosaki, Aomori 036 8560, Japan
    J Appl Physiol (1985) 93:1280-6. 2002
    We investigated whether there is a break point of creatine kinase (CK) release after daily endurance exercise and whether CK response depends on individual physical characteristics...
  75. ncbi Increased protein oxidation and decreased creatine kinase BB expression and activity after spinal cord contusion injury
    Marina Aksenova
    Department of Pharmacology, University of Kentucky, Lexington 40506 0055, USA
    J Neurotrauma 19:491-502. 2002
    ..spinal cord tissue was associated with changes in activity and expression of an oxidative sensitive enzyme, creatine kinase BB, which plays an important role in the maintenance of ATP level in the CNS tissue...
  76. ncbi Administration of memantine and imipramine alters mitochondrial respiratory chain and creatine kinase activities in rat brain
    Gislaine Z Réus
    Laboratório de Neurociências and Instituto Nacional de Ciência e Tecnologia Translacional em Medicina INCT TM, Programa de Pos Graduacao em Ciencias da Saude, Unidade Academica de Ciencias da Saude, Universidade do Extremo Sul Catarinense, Criciuma, SC, 88806 000, Brazil
    J Neural Transm 119:481-91. 2012
    ..In the present study, we evaluated the creatine kinase and mitochondrial respiratory chain activities after acute and chronic treatments with memantine (N-methyl-D: ..
  77. ncbi The creatine kinase response to eccentric exercise with atorvastatin 10 mg or 80 mg
    Amy K Kearns
    Division of Cardiology, Henry Low Heart Center, Hartford Hospital, 87 Seymour Street, Hartford, CT 06102, USA
    Atherosclerosis 200:121-5. 2008
    ..are well tolerated by most patients, but can produce a variety of skeletal muscle problems including myalgia, creatine kinase (CK) elevations and clinically important rhabdomyolysis...
  78. pmc Effects of creatine and β-guanidinopropionic acid and alterations in creatine transporter and creatine kinases expression in acute seizure and chronic epilepsy models
    Dae Won Kim
    Department of Biomedical Sciences, College of Life Science, Hallym University, Chunchon Kangwon Do 200 702, Republic of Korea
    BMC Neurosci 11:141. 2010
  79. pmc Liver aminotransferases are elevated with rhabdomyolysis in the absence of significant liver injury
    Kathryn Weibrecht
    Division of Medical Toxicology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA, USA
    J Med Toxicol 6:294-300. 2010
    ..Aminotransferase abnormalities, particularly AST, are common in the setting of rhabdomyolysis. AST concentrations decrease in parallel to CPK, suggesting skeletal muscle may be a significant source of AST elevation in these patients...
  80. ncbi IL6 (-174) and TNFA (-308) promoter polymorphisms are associated with systemic creatine kinase response to eccentric exercise
    Chen Yamin
    Department of Genetics and Molecular Biology, Zinman College of Physical Education and Sport Sciences at the Wingate Institute, 42902 Netanya, Israel
    Eur J Appl Physiol 104:579-86. 2008
    ..The inflammatory system has an important role in muscle injury and repair. Serum creatine kinase (CK) is often used as systemic biomarker representing muscle damage...
  81. ncbi Rhabdomyolysis in falciparum malaria--a series of twelve cases (five children and seven adults)
    Saroj K Mishra
    Department of Internal Medicine, Ispat General Hospital, Rourkela 769 005, Orissa, India
    Trop Doct 40:87-8. 2010
    ..Taylor and Prosser reported a single case of rhabdomyolysis with renal failure. We report on 12 patients with evidence of skeletal muscle injury in severe malaria from Rourkela, Orissa State, India...
  82. ncbi Progressive decrease of phosphocreatine, creatine and creatine kinase in skeletal muscle upon transformation to sarcoma
    Subrata Patra
    Department of Biological Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Kolkata, India
    FEBS J 275:3236-47. 2008
    In vertebrates, phosphocreatine and ATP are continuously interconverted by the reversible reaction of creatine kinase in accordance with cellular energy needs...
  83. ncbi L-2-hydroxyglutaric acid inhibits mitochondrial creatine kinase activity from cerebellum of developing rats
    Cleide G da Silva
    Department of Biochemistry, Institute of Basic Sciences and Health, UFRGS, Av Ramiro Barcellos 2600, Porto Alegre, RS, Brazil
    Int J Dev Neurosci 21:217-24. 2003
    ..In the present study, we investigated the effect of LGA, at 0.25-5mM concentrations, on total creatine kinase (tCK) activity from cerebellum, cerebral cortex, cardiac muscle and skeletal muscle homogenates of 30-day-old ..
  84. ncbi Short-term immobilization after eccentric exercise. Part II: creatine kinase and myoglobin
    Stephen P Sayers
    Human Physiology Laboratory, Department of Health Sciences, Boston University, Sargent College of Health and Rehabilitation Sciences, 635 Commonwealth Avenue, Boston, MA 02215, USA
    Med Sci Sports Exerc 35:762-8. 2003
    Although creatine kinase (CK) is commonly used as a marker of muscle damage, there is large variability in the response to exercise...
  85. ncbi In vitro complex formation between the octamer of mitochondrial creatine kinase and porin
    D Brdiczka
    Faculty of Biology, University of Konstanz, Germany
    J Biol Chem 269:27640-4. 1994
    An interaction of mitochondrial creatine kinase with purified outer mitochondrial porin (voltage-dependent anion channel) was shown by co-sedimentation assays as well as by gel permeation chromatography...
  86. ncbi Retrospective study of a large population of patients with asymptomatic or minimally symptomatic raised serum creatine kinase levels
    Alessandro Prelle
    Istituto di Clinica Neurologica, Ospedale Maggiore IRCCS, Milano, Italy
    J Neurol 249:305-11. 2002
    A retrospective evaluation of asymptomatic subjects with persistent elevation of serum creatine kinase (CK) levels (hyperCKemia) was made in order to verify the presence of subclinical myopathy or idiopathic hyperCKemia and to define the ..
  87. ncbi Antioxidant supplementation and subsequent oxidative stress of horses during an 80-km endurance race
    C A Williams
    Department of Animal Science, Rutgers The State University of New Jersey, Cook College, New Brunswick 08901, USA
    J Anim Sci 82:588-94. 2004
    ..Plasma was assayed for lipid hydroperoxides, alpha-tocopherol, total ascorbate, albumin, creatine kinase (CK), and aspartate aminotransferase (AST)...
  88. ncbi Effect of transportation stress on heat shock protein 70 concentration and mRNA expression in heart and kidney tissues and serum enzyme activities and hormone concentrations of pigs
    Hong Yu
    Key Laboratory of Animal Physiology and Biochemistry, Department of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
    Am J Vet Res 68:1145-50. 2007
    ..To determine the enzymatic and hormonal responses, heat shock protein 70 (Hsp70) production, and Hsp70 mRNA expression in heart and kidney tissues of transport-stressed pigs...
  89. ncbi Serum creatine kinase levels in overt and subclinical hypothyroidism
    Zeliha Hekimsoy
    Celal Bayar University, Medical Faculty, Department of Internal Medicine, Division of Endocrinology and Metabolism, Manisa, Turkey
    Endocr Res 31:171-5. 2005
    The aims of this prospective study were to determine serum levels of creatine kinase (CK) in overt and subclinical hypothyroidism; to investigate the change in CK levels with treatment; and to evaluate the relationship between free ..
  90. ncbi Safety evaluation of telbivudine
    David Yiu Kuen But
    University of Hong Kong, Queen Mary Hospital, Department of Medicine, Pokfulam Road, Hong Kong SAR, China
    Expert Opin Drug Saf 9:821-9. 2010
    ..5- to 6.6-log copies/ml hepatitis B DNA reduction at 12 weeks in an early viral kinetic study, a potency comparable to entecavir. It is also one of the few drugs in the treatment of CHB under FDA pregnancy Category B...
  91. ncbi Alpha lipoic acid protects heart against myocardial ischemia-reperfusion injury through a mechanism involving aldehyde dehydrogenase 2 activation
    Lan He
    Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha 410078, China
    Eur J Pharmacol 678:32-8. 2012
    ..In a Langendorff model of ischemia-reperfusion in rats, cardiac function, activities of creatine kinase (CK) and ALDH2, contents of 4-hydroxy-2-nonenal (4-HNE) and malondialdehyde (MDA) were measured...
  92. pmc Effectiveness of post-match recovery strategies in rugby players
    N D Gill
    Waikato Institute of Technology, School of Sport and Exercise Science, Hamilton, New Zealand
    Br J Sports Med 40:260-3. 2006
    To examine the effectiveness of four interventions on the rate and magnitude of muscle damage recovery, as measured by creatine kinase (CK).
  93. ncbi Serum enzyme monitoring in sports medicine
    Paola Brancaccio
    Seconda Universita di Napoli, Department of Experimental Medicine Sport Medicine, Center of Excellence of Cardiovascular Disease, Napoli, Italy
    Clin Sports Med 27:1-18, vii. 2008
    ..Serum total LDH and specific isozyme activities change with the training status of the athlete. Variation in LDH isozymes profile might have a role in studying muscle response to training...
  94. pmc Expression and distribution of heat shock proteins in the heart of transported pigs
    Endong Bao
    College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China
    Cell Stress Chaperones 13:459-66. 2008
    ..Whether this distinct depletion of Hsps contributes to an increased susceptibility to acute heart failure and the sudden death syndrome in transported pigs should be elucidated in future experiments...
  95. pmc Rhabdomyolysis in community acquired bacterial sepsis--a retrospective cohort study
    Anita A Kumar
    Department of General Medicine, Sri Ramachandra University, Tamilnadu, Chennai, India
    PLoS ONE 4:e7182. 2009
    ..We report the pattern of infecting bacterial pathogens and associated causal factors in a South-Indian cohort. DESIGN, SETTING, PARTICIPANTS #ENTITYSTARTX00026;..
  96. ncbi Protection of skeletal muscles from ischemic injury: low-level laser therapy increases antioxidant activity
    Dorit Avni
    Department of Zoology, The George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
    Photomed Laser Surg 23:273-7. 2005
    ..The aim of this study was to investigate the effect of low-level laser therapy (LLLT) on ischemic-reperfusion (I-R) injury in the gastrocnemius muscle of the rat...
  97. ncbi Structural characterization and tissue-specific expression of the mRNAs encoding isoenzymes from two rat mitochondrial creatine kinase genes
    R M Payne
    Department of Pediatrics, Washington University School of Medicine, St Louis, MO 63110
    Biochim Biophys Acta 1089:352-61. 1991
    b>Creatine kinase (CK; EC isoenzymes play prominent roles in energy transduction...
  98. ncbi The effect of whole-body cryostimulation on lysosomal enzyme activity in kayakers during training
    Alina Wozniak
    The Chair of Medical Biology, Nicolaus Copernicus University in Torun, Collegium Medicum, Karłowicza 24, 85 092 Bydgoszcz, Poland
    Eur J Appl Physiol 100:137-42. 2007
    ..enzyme activity: acid phosphatase (AcP), arylsulphatase (ASA) and cathepsin D (CTS D), as well as on the creatine kinase (CK), and the cortisol concentration in the serum of kayakers during training were studied...
  99. ncbi Clinical heterogeneity in dysferlinopathy
    Hidetsugu Ueyama
    Third Department of Internal Medicine, Oita Medical University
    Intern Med 41:532-6. 2002
    ..To clarify the clinical heterogeneity and genotype-phenotype correlation in dysferlinopathy...
  100. ncbi Unraveling multistate unfolding of rabbit muscle creatine kinase
    Irina M Kuznetsova
    Institute of Cytology, Russian Academy of Sciences, 194064 St Petersburg, Russia
    Biochim Biophys Acta 1596:138-55. 2002
    GdmCl-induced unfolding of rabbit muscle creatine kinase, CK, has been studied by a variety of physico-chemical methods including near and far UV CD, SEC, intrinsic fluorescence (intensity, anisotropy and lifetime) as well as intensity ..
  101. pmc Analyzing the functional properties of the creatine kinase system with multiscale 'sloppy' modeling
    Hannes Hettling
    Centre for Integrative Bioinformatics VU, VU University Amsterdam, Amsterdam, The Netherlands
    PLoS Comput Biol 7:e1002130. 2011
    In this study the function of the two isoforms of creatine kinase (CK; EC in myocardium is investigated...

Research Grants66

  1. Infant Screening and Diagnosis of Duchenne Muscular Dys*
    Paul Fernhoff; Fiscal Year: 2007
    ..A bioluminescence assay will be used to measure creatine kinase (CK) levels and multiplex ligation-dependent probe amplification (MLPA) techniques to detect deletions and ..
  2. MR Monitoring of PTC124 Treatment in DMD
    KRISTA H VANDENBORNE; Fiscal Year: 2010
    ..relatively noninvasive method of monitoring the disease process is unreliable and measures blood levels of creatine kinase. We have evidence in mouse models of both DMD and limb girdle muscular dystrophy, that magnetic resonance ..
  3. PET Imaging of Myocardial Oxidative Stress in T2DM
    KOORESH ISAAC SHOGHI; Fiscal Year: 2013
    ..energetics as defined by the phosphocreatine and adenosine triphosphate concentrations and their ratio and creatine kinase flux using [31]P-MRS;and left ventricular structure/ work by MRI and superoxide levels by optical imaging of ..
  4. Striated Muscle Gene Transcription and Regulatory Gene Cassettes for Gene Therapy
    ..Both components focus on the mouse M-creatine kinase gene...
    Veronique L Roger; Fiscal Year: 2012
    ..As troponin is more sensitive than previous biomarkers (creatine kinase and its MB fraction), it was hypothesized that the new definition would change the number and case mix of MI...
  6. Intravenous Protein Therapy for Treatment of Congenital Muscular Dystrophy
    Bradley L Hodges; Fiscal Year: 2010
    ..myofiber degeneration, was associated with increased expression of the alpha7 integrin, and reduced serum creatine kinase activity. Subsequent preliminary data has demonstrated that multiple systemic doses of 1...
  7. Discovering tissue-specific biomarkers of radiation injury in SILAC-labeled mice
    Amanda G Paulovich; Fiscal Year: 2013
    ..Precedents include the troponins in myocardial infarction, transaminases in liver injury, creatine kinase in muscle injury, and lipase in pancreatic processes...
  8. In vivo Studies of Creatine Kinase Kinetics by 31P Magnetization Transfer MRS
    Adil Bashir; Fiscal Year: 2013
    ..The creatine kinase (CK) system acts as the primary ATP reserve for the heart and instantaneously supplies ATP for its ..
  9. Preventing Anthracycline Cardiovascular Toxicity with Statins
    William Gregory Hundley; Fiscal Year: 2013
    ..of side effects related to the administration of statins including diabetes, cognitive decline, myalgias and creatine kinase elevations...
  10. Intermediate Filaments & Costamere Structure & Function
    Robert J Bloch; Fiscal Year: 2010
    ..The K19-null mutation in mice also leads to mitochondrial mislocalization, increased serum creatine kinase levels and reduced contractile force, suggesting that the absence of IFs containing K19 causes a mild ..
  11. PGC-1 &Mitichondrial Dysfunction in Diabetes
    Lawrence J Mandarino; Fiscal Year: 2012
    ..resistant human muscle have decreased respiration during conditions of increased energy demand simulated by a creatine kinase "energy clamp". 2...
  12. MR Spectroscopy to Evaluate Liver Repopulation by Transplanted Hepatocytes
    Chandan Guha; Fiscal Year: 2009
    ..To address this issue, we hypothesized that the expression of brain isozyme of creatine kinase (CK-B), an ATP buffering enzyme that is expressed in muscle and brain but absent from the liver, as a ..
    Young Hoon Sung; Fiscal Year: 2013
    ..Thus, METH users may have a distinct pattern of bioenergetic dysfunction related to creatine kinase (CK), the enzyme that is directly involved in regeneration of adenosine triphosphate (ATP) from PCr...
  14. Mechanisms Underlying Sex-Specific Effects of Creatine Supplementation on Depress
    PATRICIA JOAN ALLEN; Fiscal Year: 2011 females may be explained by the phosphorylating action of estrogens or estrogenic metabolites upon creatine kinase, an enzyme that stimulates the consumption of brain energy stores...
  15. System for DNA-Guided Optimization and Personalization of Statin Therapy
    GUALBERTO RUANO; Fiscal Year: 2013
    ..effects, including myalgia (muscle aches, cramps, weakness) and myopathy (muscular injury monitored by serum creatine kinase (CK) elevation)...
  16. Bioenergetics in Hypertrophied and Remodeled Left Ventricle
    Jianyi Zhang; Fiscal Year: 2013
    ..We hypothesize that there is transmural gradient of reserves of ATP production rate via both creatine kinase (CK) and ATPase, which is the lowest in ENDO that results in the ENDO vulnerability in LVH hearts...
  17. NT-3 gene therapy to improve peripheral nerve function induced by genetic defect
    Zarife Sahenk; Fiscal Year: 2012
    ..NT-3 under control of the CMV promoter versus the muscle specific truncated creatine kinase (tMCK) promoter both given at high dose (1X 1011 vg)...
  18. Altered Creatine Kinase Energy Metabolism in Human Heart Failure
    Robert G Weiss; Fiscal Year: 2013
    ..Our focus is on the creatine kinase (CK) reaction which generates ATP at the contractile elements, is the major cardiac energy reserve ..
  19. Protein Damage and Repair in the Brain
    DANA WILLIAM ASWAD; Fiscal Year: 2012
    ..We will initially focus on creatine kinase B (brain specific isoform) and the 70 kDa heat shock cognate protein (HSC70) which are among the 22 proteins ..
  20. Vascular Delivery of alpha-Sarcoglycan for LGMD2D
    JERRY ROY MENDELL; Fiscal Year: 2013 LGMD2D to replace the human alpha-sarcoglycan gene (hSGCA) delivered by rAAV1 under control of a muscle creatine kinase promoter was recently completed. Gene expression was observed for as long as 6 months...
  21. Novel 31P MRS Approach for Measuring Abnormal Bioenergetics in Schizophrenia
    Fei Du; Fiscal Year: 2012
    ..anesthetic rats'brains demonstrate that measurements of cerebral energy metabolic rates catalyzed by the creatine kinase (CK) and ATPase enzymes by 31P magnetization transfer (MT) spectroscopy provide more sensitive and ..
  22. Phase 2b Study of PTC124 in Duchenne/Becker Muscular Dystrophy (IND 68,431)
    JAY A BARTH; Fiscal Year: 2012
    ..functional dystrophin protein in muscle, decreasing eccentric contraction injury and reducing muscle-derived creatine kinase (CK) in the serum...
  23. Rapid homogeneous antibody-based detection of proteins
    Tomasz Heyduk; Fiscal Year: 2010
    ..pincer assays for six markers of cardiac disease (troponin I, B-type natriuretic peptide (BNP), myoglobin, creatine kinase (CK-MB), C-reactive protein (CRP), and D-dimer) for research market...
  24. Bioenergetics and fatigability in older individuals
    Robert G Weiss; Fiscal Year: 2013
    ..magnetization transfer techniques, developed in our laboratory, to determine the rate of ATP production via creatine kinase, the primary muscle energy reserve reaction...
  25. 31P magnetization transfer study of bipolar disorder
    Chun S Zuo; Fiscal Year: 2010
    ..Furthermore, molecular genetics studies have reported decreases in the mRNA expression of creatine kinase (CK), the enzyme that facilitates the reversible transfer of a phosphate group from PCr to ADP, resulting in ..
    JAMES BRYANT HURLEY; Fiscal Year: 2013
    ..ATP in the cell body is converted to phosphocreatine by a mitochondrial creatine kinase. This traps the energy in a form that is protected from consumption by ion pumps as it diffuses to the ..
  27. Intravenous Protein Therapy for the Treatment of Duchenne Muscular Dystrophy
    BRADLEY HODGES; Fiscal Year: 2009
    ..localized around myofibers for at least 4 weeks, substantially reduced myofiber degeneration, reduced serum creatine kinase and was associated with increased expression of the alpha7 integrin and utrophin...
    Gabor Huszar; Fiscal Year: 1999
    ..infertility in the physician's office and in the Andrology laboratory we propose to study: 1) Sperm creatine kinase (CK) activity and CK-M isoform ratios, objective biochemical markers of sperm cellular maturity and ..
  29. Dystrophin restoration in two animal models of Duchenne Muscular Dystrophy
    LOUISE RODINO; Fiscal Year: 2009 AAV serotype that crosses the vascular barrier carrying micro-dystrophin driven by a truncated muscle creatine kinase (MCK) promoter delivered through the femoral artery of the mdx mouse...
  30. Noninvasive assessment of skeletal muscle integrity and function with statin use
    Jill Slade; Fiscal Year: 2009
    ..Currently serum creatine kinase (CK) or muscle biopsies are used to verify statin induced myopathy...
    MARC VISNER; Fiscal Year: 1991
    ..g. heavy-chain myosin subtypes), myocardial energetics (e.g. creatine kinase), or the cellular response to injury (e.g. stress proteins, catalase) will also be studied...
  32. Cardiac Troponin Testing for Myocardial Infarction
    Fred Apple; Fiscal Year: 2001
    ..for the sensitive and specific detection of acute myocardial infarction (AMI), supporting the replacement of creatine kinase MB (CKMB) as the new standard biochemical marker for AMI...
    Gabor Huszar; Fiscal Year: 2001
    ..we will develop the preservation and shipping of samples for tests routinely used in our laboratory: sperm creatine kinase (CK)-activity, CK-M isoform ratio, and CK- immunostaining for CK-B in order to highlight immature sperm, and ..
    THOMAS ALBER; Fiscal Year: 1990
    ..series of enzymes: adenylate kinase isoenzymes from rabbit and calf; nucleoside diphosphokinase from yeast; creatine kinase isoenzymes from rabbit and calf; thiamin pyrophosphokinase from porcine brain...
  35. MR Studies of Myocardial Creatine Kinase Metabolism
    PAUL BOTTOMLEY; Fiscal Year: 2007
    ..creatine,Cr) is also reduced, while animal studies show that the rate of generating ATP from PCr via the creatine kinase reaction (CKR) is reduced in dysfunctional myocardium post-MI...
    William Craigen; Fiscal Year: 2001
    ..The kinases known to bind VDACs include hexokinase, glucokinase, glycerol kinase, and mitochondrial creatine kinase. This group of kinases is important in a variety of metabolic functions including glycolysis, the ..
  37. Biomechanical Effects of Industrial Eccentric Exertions
    Robert Radwin; Fiscal Year: 2004
    ..Biochemical measures of blood creatine kinase (CK), and anatomical measures using T2 changes in an MRI of the forearms will be compared with biomechanical ..
  38. Multi-analyte Biosensor for Emerging Cardiac Markers
    CHARLES PENNINGTON; Fiscal Year: 2001
    ..Phase I work will demonstrate the sensor platform with creatine kinase MB and P-selectin...
  39. Implementing Newborn Screening for Duchenne Muscular Dystrophy in the Community
    Jerry Mendell; Fiscal Year: 2009
    ..screening program (NBS) for Duchenne muscular dystrophy (DMD) was established and included: the range of creatine kinase (CK) observed on >30,000 anonymous samples, a successful method to measure CK isoenzymes, with particular ..
  40. Preclinical Trials of NFkappaB Inhibition in the Treatment of Muscular Dystrophy
    C Carlson; Fiscal Year: 2009
    ..the expression of inflammatory cytokines in plasma and muscle extracts, resting membrane potential, plasma creatine kinase levels, skeletal muscle fibrosis, and histological determinations of the total number of fibers, the ..
    Frank LaFerla; Fiscal Year: 2001
    ..The muscle creatine kinase promotor, which has been used by other investigators to direct expression to skeletal muscle of transgenic ..
    PATTI NEMETH; Fiscal Year: 1993
    ..biochemical analyses of the constituent muscle fibers include: activities of enzymes (adenylokinase, creatine kinase, lactate dehydrogenase, malate dehydrogenase and beta-hydroxyacyl CoA dehydrogenase) and concentrations of ..
  43. Diagnosis of Cardiac Viability by Functional Ultrasound
    Marek Belohlavek; Fiscal Year: 2005
    ..measures are a) adenosine triphosphate turnover, b) creatine phosphate/ inorganic phosphate ratio, and c) creatine kinase activity...
    GEORGE KENYON; Fiscal Year: 1999
    DESCRIPTION: This project focuses on investigation of structure-function relationships in creatine kinase (C.K.), a phosphotransferase enzyme. The C.K...
    Dana Abendschein; Fiscal Year: 1991
    ..However, conventional methods including detection of changes in activity of total creatine kinase (CK) or its isoenzymes in plasma are neither rapid nor sufficiently sensitive to permit reliable, early ..
    HENRYK WISNIEWSKI; Fiscal Year: 1991
    ..Medina (selenium binding proteins); B. O'Malley (steroid receptors and transcription factors); B. Perryman (creatine kinase from plasma); S. Rich (immunoregulatory lymphokines and factors); S...
  47. High Content Screening for Muscular Dystrophy
    Herman Vandenburgh; Fiscal Year: 2007 well as myofiber formation; (2) determine the damaging effects of DmBAM eccentric contractions based on creatine kinase release, force generation, and muscle fiber morphology; and (3) test the ability of Prednisone to attenuate ..
    Michael Chapman; Fiscal Year: 2002
    ..Arginine kinase (AK), the "primordial" phosphagen kinase, will be used as a model to understand human creatine kinase (CK) whose active site has eluded detailed characterization...
    STEPHEN KONIECZNY; Fiscal Year: 1993
    ..including alpha-actin, myosin heavy chain, myosin light chain, alpha-tropomyosin, troponins C,I and T, and M-creatine kinase. Although these genes are expressed in a tissue-specific fashion, the molecular regulatory mechanisms ..
    Joseph Billadello; Fiscal Year: 1992
    b>Creatine kinase (EC is essential for maintaining proper intracellular ATP:ADP ratios and phosphocreatine pool size and plays a prominent role in cardiac and skeletal muscle energy metabolism...
    STEPHEN HAUSCHKA; Fiscal Year: 1992
    ..As a model system we will continue delineating the cis- and trans regulatory components of the mouse muscle creatine kinase (MCK) gene which are essential for its expression in cardiac muscle cultures and in the hearts of transgenic ..
    David Holtzman; Fiscal Year: 1999
    ..The first hypothesis is that the phosphocreatine (PCr)/creatine kinase (CK)/ATP system, including the mitochondrial CK (MiCK), closely couples ATP synthesis to the variable ATP ..
  53. The role of creatine in health and disease
    William Craigen; Fiscal Year: 2009
    ..In these organs, creatine and phosphocreatine, together with creatine kinase isozymes and ATP/ADP, provide the major reserve of energy...
  54. Diaphragm Mitochondrial Alterations in Sepsis
    Leigh Callahan; Fiscal Year: 2004
    ..specific performance of complexes within the electron transport chain, assess sarcomeric mitochondrial creatine kinase activity, and perform a metabolic control analysis...
    Arnold Strauss; Fiscal Year: 2001
    ..Our proposed focus is regulation of the two human mitochondrial creatine kinase (MtCK) genes...
  56. Novel ALT Isoenzymes and Their Diagnositc Application
    Da Wei Gong; Fiscal Year: 2003
    ..of an isoenzyme in serum may have more diagnostic value than total enzyme activity, as exemplified by creatine kinase-MB for cardiac infarction and bone-specific alkaline phosphatase for bone disease...
    HOWARD WEINBERGER; Fiscal Year: 1999
    ..It has been thought that creatine kinase plays a crucial role in this process by providing an "energy reserve" function, however the compensatory ..
    Brian Pentecost; Fiscal Year: 1993
    ..One of the earliest biochemical events in this process is a rapid but transient increase in creatine kinase B (CKB) synthesis in both the endometrium and myometrium...
    MOHAMMED MATLIB; Fiscal Year: 2000
    ..The changes in high-energy phosphate metabolism, free energy release from ATP breakdown and creatine kinase (CK) reaction velocity in isolated hearts during increased workload will be determined employing 31P-NMR ..
    Christine Seidman; Fiscal Year: 2004
    ..biochemical and molecular biologic tools to test the hypothesis that decreased energy reserve via the creatine kinase system impairs contractile mutated G/a0 subunits that develop dilated cardiomyopathy with compensatory ..
    Langdon Miller; Fiscal Year: 2007
    ..protein that improves strength, decreases eccentric contraction injury to muscle, and reduces serum creatine kinase levels. Toxicology evaluations and Phase 1 clinical studies of PTCI24 in healthy volunteers are now complete...
  62. Modulating IBM pathology in transgenic mice
    Frank LaFerla; Fiscal Year: 2005
    ..The investigator has derived transgenic mice in which the muscle creatine kinase (MCK) promoter drives betaAPP expression in skeletal muscle...
    RICHARD VAN WAGENEN; Fiscal Year: 1999
    ..Creatine is then phosphorylated by the enzyme creatine kinase with the simultaneous consumption of ATP...
    Peter Gillespie; Fiscal Year: 2004
    ..that are targeted to bundles, and it will be determined whether hair bundles possess unique isozymes of creatine kinase or adenylate kinase, two enzymes that serve to maintain high ATP levels...
    John Loike; Fiscal Year: 1992
    ..Moreover, L6 myoblasts appear to contain an inactive form of creatine kinase which becomes activated during differentiation...
    Paul Okunieff; Fiscal Year: 1992 energy of hydrolysis); Mg++; free nucleotide diphosphates ((NDP)); T, of Pi and PCr; and ATPase and CK (creatine kinase) reaction rates will all be measured concurrently...